##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578594_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9183687 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.644682903500524 31.0 31.0 34.0 27.0 34.0 2 30.938815641256067 31.0 31.0 34.0 27.0 34.0 3 31.037677024489184 31.0 31.0 34.0 27.0 34.0 4 34.484258773192074 37.0 35.0 37.0 30.0 37.0 5 34.53721822183182 37.0 35.0 37.0 30.0 37.0 6 34.531160088535245 37.0 35.0 37.0 30.0 37.0 7 34.436410997021135 37.0 35.0 37.0 30.0 37.0 8 34.399418011524126 37.0 35.0 37.0 30.0 37.0 9 35.832698239824595 39.0 35.0 39.0 30.0 39.0 10-11 35.80813887711983 39.0 35.0 39.0 30.0 39.0 12-13 35.87402494226992 39.0 35.0 39.0 30.0 39.0 14-15 36.90854827696109 40.0 36.0 41.0 31.0 41.0 16-17 36.87359325290594 40.0 36.0 41.0 30.5 41.0 18-19 36.86815600313905 40.0 36.0 41.0 30.5 41.0 20-21 36.75868248776335 39.5 36.0 41.0 30.0 41.0 22-23 36.45066327935611 39.0 36.0 40.5 29.5 41.0 24-25 36.344366919299404 39.0 36.0 40.0 29.5 41.0 26-27 36.28597740754884 39.0 36.0 40.5 29.0 41.0 28-29 35.97475741496852 39.0 35.0 41.0 27.0 41.0 30-31 35.676439321157176 38.5 34.5 40.5 26.5 41.0 32-33 35.366072635097424 38.0 34.0 40.0 25.5 41.0 34-35 35.17757965836597 38.0 34.0 40.0 25.0 41.0 36-37 35.193954236462986 38.0 34.0 40.0 25.0 41.0 38-39 34.51812866662377 37.0 33.5 40.0 25.0 41.0 40-41 34.47402905826385 37.0 33.0 40.0 25.0 41.0 42-43 34.29673882613813 37.0 33.0 40.0 24.0 41.0 44-45 34.13505621435051 37.0 33.0 40.0 23.5 41.0 46-47 33.92363998250376 37.0 33.0 40.0 23.0 41.0 48-49 33.548040236998496 37.0 33.0 40.0 21.0 41.0 50-51 32.23554902295778 36.0 31.5 39.0 15.5 40.5 52-53 31.795329098215127 36.0 31.0 39.0 7.5 40.0 54-55 31.415238999325652 37.0 30.5 40.0 2.0 41.0 56-57 30.864517758499392 37.0 29.0 40.0 2.0 41.0 58-59 30.513294224857617 36.0 28.0 40.0 2.0 41.0 60-61 30.208295861999652 36.0 28.0 40.0 2.0 41.0 62-63 29.87147427825012 35.0 28.0 39.0 2.0 41.0 64-65 29.538667694140706 35.0 27.5 39.0 2.0 41.0 66-67 29.23298393118145 35.0 26.5 39.0 2.0 40.5 68-69 28.902460743707838 35.0 26.0 38.0 2.0 40.0 70-71 28.510087669581946 35.0 26.0 37.5 2.0 40.0 72-73 28.122207943280294 34.0 26.0 37.0 2.0 39.5 74-75 27.720580198345175 34.0 25.0 36.5 2.0 39.0 76-77 27.316839576523023 34.0 24.5 36.0 2.0 39.0 78-79 26.93044928469361 34.0 23.5 35.5 2.0 38.5 80-81 26.53892603264898 34.0 22.0 35.0 2.0 37.0 82-83 26.1623205364033 33.0 20.5 35.0 2.0 37.0 84-85 25.756089411583822 33.0 19.5 35.0 2.0 36.0 86-87 25.37201104523706 33.0 18.0 35.0 2.0 36.0 88-89 24.990659797094565 32.5 14.0 35.0 2.0 35.5 90-91 24.60731240078195 32.0 7.5 35.0 2.0 35.0 92-93 24.23665968798806 32.0 3.5 35.0 2.0 35.0 94-95 23.803351910839297 32.0 2.0 35.0 2.0 35.0 96-97 23.323767785204353 31.0 2.0 35.0 2.0 35.0 98-99 22.733736570072566 31.0 2.0 34.0 2.0 35.0 100-101 21.165365827472126 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5623914524053504 1101 2 0.5372975549512766 1101 3 0.5285787918670266 1101 4 0.6847256509667403 1101 5 0.620264202761966 1101 6 0.47871940835243265 1101 7 0.5623363528370575 1101 8 0.6663177224405388 1101 9 0.6425314796077615 1101 10-11 0.6543744177526136 1101 12-13 0.6170703773006778 1101 14-15 0.7626945184584528 1101 16-17 0.7040930340907039 1101 18-19 0.649934368886683 1101 20-21 0.6713572242178145 1101 22-23 0.7874743548931917 1101 24-25 0.9280668898120297 1101 26-27 0.8243628597024681 1101 28-29 0.8432649407108741 1101 30-31 0.996061858187069 1101 32-33 1.1146505599593013 1101 34-35 1.0594465584000972 1101 36-37 0.9508367549459251 1101 38-39 1.0084220664652008 1101 40-41 0.9772582931258 1101 42-43 1.1847393371730632 1101 44-45 1.098832325753527 1101 46-47 1.0553019892512765 1101 48-49 1.1211751262653067 1101 50-51 1.0704576080396535 1101 52-53 1.1769309293557129 1101 54-55 1.1432012449105073 1101 56-57 1.1533746633971198 1101 58-59 1.165071777439092 1101 60-61 1.269707692162907 1101 62-63 1.313590150562451 1101 64-65 1.2574739110660893 1101 66-67 1.1207432892042348 1101 68-69 1.3180180165006128 1101 70-71 1.2220162517630904 1101 72-73 1.33144063867611 1101 74-75 1.329007815030721 1101 76-77 1.2727271720152267 1101 78-79 1.2512681384568936 1101 80-81 1.3561431563898303 1101 82-83 1.3681310815580012 1101 84-85 1.4710817943744736 1101 86-87 1.5167392777557929 1101 88-89 1.5468750878573303 1101 90-91 1.6958416823757254 1101 92-93 1.7246672296087766 1101 94-95 1.7657943104220024 1101 96-97 1.9598891579551392 1101 98-99 2.1858260522136703 1101 100-101 2.608255922693651 1102 1 0.48732208720748105 1102 2 0.4250037054016218 1102 3 0.41871787655671255 1102 4 0.548618292489806 1102 5 0.5349057475947419 1102 6 0.45968114506895574 1102 7 0.5397773802617252 1102 8 0.6029712840904651 1102 9 0.5085826164976837 1102 10-11 0.6037683782680574 1102 12-13 0.578196023597684 1102 14-15 0.8202560891894208 1102 16-17 0.7462355526529265 1102 18-19 0.6979550293183863 1102 20-21 0.7345104971065979 1102 22-23 0.7097006616589567 1102 24-25 0.855507517787629 1102 26-27 0.9352528021076978 1102 28-29 0.7824743627069282 1102 30-31 0.9112369917072556 1102 32-33 0.9373507811961872 1102 34-35 0.9809052687979118 1102 36-37 0.8166371015056129 1102 38-39 0.9010938839792715 1102 40-41 0.8871350995618172 1102 42-43 1.0729443126642693 1102 44-45 1.0359289632492121 1102 46-47 0.9999095542838958 1102 48-49 1.0686573235732268 1102 50-51 0.9241344266559679 1102 52-53 0.9973175126838001 1102 54-55 0.9882645871112246 1102 56-57 0.7641987661766336 1102 58-59 0.9406300451315595 1102 60-61 0.9614266963051357 1102 62-63 0.9621030017071242 1102 64-65 1.0053387632074156 1102 66-67 1.0074830623820041 1102 68-69 1.1470732060038813 1102 70-71 1.0537324261850145 1102 72-73 1.0655321803596038 1102 74-75 1.2014806744931974 1102 76-77 1.1143281244870415 1102 78-79 1.095618579865473 1102 80-81 1.2120445820872092 1102 82-83 1.3304469818536617 1102 84-85 1.361542533973516 1102 86-87 1.3969539810473464 1102 88-89 1.4964267497043657 1102 90-91 1.4744346861585704 1102 92-93 1.4291623977803027 1102 94-95 1.49630254509335 1102 96-97 1.7564884802116545 1102 98-99 1.862733901978217 1102 100-101 2.244426631476067 1103 1 0.42922719624507977 1103 2 0.33222633404783153 1103 3 0.2864636012995625 1103 4 0.38016148880009126 1103 5 0.37640437075253175 1103 6 0.26647725728071947 1103 7 0.4830334376524732 1103 8 0.3803371708269836 1103 9 0.6512784155999469 1103 10-11 0.5103663384477954 1103 12-13 0.357748680669701 1103 14-15 0.6666215743385919 1103 16-17 0.6074781579871953 1103 18-19 0.6078084370136239 1103 20-21 0.7500220828127055 1103 22-23 0.7583871873265196 1103 24-25 0.6722886145489326 1103 26-27 0.8553957874195248 1103 28-29 0.7627322756230583 1103 30-31 0.6636415742249611 1103 32-33 0.8713080096450696 1103 34-35 0.8631333027650925 1103 36-37 0.8492760403640176 1103 38-39 0.5618149655694964 1103 40-41 0.7120951116720349 1103 42-43 0.5090530454979572 1103 44-45 0.6747389725770958 1103 46-47 0.7257226222338247 1103 48-49 0.7789210762459575 1103 50-51 0.676535804744347 1103 52-53 0.6667575019058916 1103 54-55 0.7283798558322019 1103 56-57 0.6378155222868855 1103 58-59 0.7110842477205175 1103 60-61 0.6536642132008907 1103 62-63 0.7144242575769546 1103 64-65 0.8741964536396729 1103 66-67 0.7697863051094274 1103 68-69 0.8945417556498789 1103 70-71 0.8903998954904004 1103 72-73 0.9246320331913047 1103 74-75 1.041260592219757 1103 76-77 0.9241257303189165 1103 78-79 1.0099680488404275 1103 80-81 0.9683275405472997 1103 82-83 1.095280580949808 1103 84-85 1.0915396907603245 1103 86-87 1.2767363807709593 1103 88-89 1.3143961154748673 1103 90-91 1.053340668719006 1103 92-93 1.1471615969026807 1103 94-95 1.1588877661159884 1103 96-97 1.380843458163401 1103 98-99 1.5079536600759589 1103 100-101 1.8180607764967078 1104 1 0.2013494925179451 1104 2 0.24052958052012485 1104 3 0.20669273970080226 1104 4 0.2365423225956036 1104 5 0.10554604427841241 1104 6 0.08270410899661584 1104 7 0.26473766150375866 1104 8 0.2169254788056847 1104 9 0.37157747466361 1104 10-11 0.40416854296237403 1104 12-13 0.28399871528043263 1104 14-15 0.42406251724948874 1104 16-17 0.6273041338489094 1104 18-19 0.6709366706694553 1104 20-21 0.5319016956536018 1104 22-23 0.44450505510430816 1104 24-25 0.4966744803946739 1104 26-27 0.5413676888224472 1104 28-29 0.6761372972131525 1104 30-31 0.5868447180816858 1104 32-33 0.7446639860857189 1104 34-35 0.7154864826589318 1104 36-37 0.8634268357395172 1104 38-39 0.6461299430508518 1104 40-41 0.6222044927595007 1104 42-43 0.7410275686460253 1104 44-45 0.9366022016083733 1104 46-47 0.8134089223428163 1104 48-49 0.7447525545841174 1104 50-51 0.7944407523583834 1104 52-53 0.5632244388019316 1104 54-55 0.7123031097943908 1104 56-57 0.7439341116459133 1104 58-59 0.7069142073818888 1104 60-61 0.7276196334646237 1104 62-63 0.619410272046057 1104 64-65 0.8115061191084578 1104 66-67 0.5987224118877883 1104 68-69 0.7098085407341799 1104 70-71 0.8371049961467882 1104 72-73 0.6213014603257214 1104 74-75 0.7835123340133805 1104 76-77 0.881611478390294 1104 78-79 0.95957144485903 1104 80-81 0.824147135169671 1104 82-83 0.84742008329928 1104 84-85 0.7646530425023705 1104 86-87 0.9149776132093379 1104 88-89 1.006329172873297 1104 90-91 1.0690122105339412 1104 92-93 1.0351428223657706 1104 94-95 1.1182374312379721 1104 96-97 1.2939656366898724 1104 98-99 1.5316324881832841 1104 100-101 1.7729330020797818 1105 1 -0.08053412923154468 1105 2 0.1027226172695137 1105 3 0.10071604778725884 1105 4 0.04365403692436587 1105 5 0.14133685544165075 1105 6 0.14942594138110366 1105 7 0.28632497975853255 1105 8 0.34619152800763686 1105 9 0.20887963083335137 1105 10-11 0.26312104218136056 1105 12-13 0.18551982162972536 1105 14-15 0.06810192394246428 1105 16-17 0.28002975511272865 1105 18-19 0.16568335621530395 1105 20-21 0.19288622816463885 1105 22-23 0.29659229215950234 1105 24-25 0.42144882271605866 1105 26-27 0.4577771201927021 1105 28-29 0.5825007633116215 1105 30-31 0.490505797738372 1105 32-33 0.6211712548076562 1105 34-35 0.4501747729825638 1105 36-37 0.6489561722703954 1105 38-39 0.5119904347659912 1105 40-41 0.45973323600075133 1105 42-43 0.706368959109092 1105 44-45 0.7375397416238982 1105 46-47 0.45766618742327836 1105 48-49 0.6201728412632335 1105 50-51 0.640943430640398 1105 52-53 0.43547891439934006 1105 54-55 0.5922275224343991 1105 56-57 0.7310939456987349 1105 58-59 0.7482783104677573 1105 60-61 0.7939326090719199 1105 62-63 0.7858903427103527 1105 64-65 0.9088884052497228 1105 66-67 0.820379766146484 1105 68-69 0.849995711764425 1105 70-71 0.84289935313409 1105 72-73 0.6996206864534251 1105 74-75 0.6636587030656216 1105 76-77 0.592304186971603 1105 78-79 0.7255949716517449 1105 80-81 0.7603859897924039 1105 82-83 0.8719370381429599 1105 84-85 0.8577041458493362 1105 86-87 0.9291516427329434 1105 88-89 0.8615633668353091 1105 90-91 1.0942785332274134 1105 92-93 1.15019044315261 1105 94-95 1.2331921344768446 1105 96-97 1.4074055164927373 1105 98-99 1.6828698307761947 1105 100-101 2.0488231484934047 1106 1 0.15424197556420793 1106 2 0.2583822129927711 1106 3 0.22072530100325594 1106 4 0.10563569857554 1106 5 0.27247647659774543 1106 6 0.19600006940646608 1106 7 0.29739006964089754 1106 8 0.27750635102016474 1106 9 0.2896346051120915 1106 10-11 0.16846972725464582 1106 12-13 0.22590414659973135 1106 14-15 0.11779189907782239 1106 16-17 0.23469275592440653 1106 18-19 0.22428762103400857 1106 20-21 0.1821664001193355 1106 22-23 0.26468080090965174 1106 24-25 0.46460364654091535 1106 26-27 0.34240046482736375 1106 28-29 0.3464983157355306 1106 30-31 0.35304540692556685 1106 32-33 0.3373341418715441 1106 34-35 0.29236939471010714 1106 36-37 0.4153107886486538 1106 38-39 0.42093285353461596 1106 40-41 0.35351203077531324 1106 42-43 0.5264447812528985 1106 44-45 0.5215651919647186 1106 46-47 0.5689514705753567 1106 48-49 0.6462560432950895 1106 50-51 0.5652728101304945 1106 52-53 0.5539352509078839 1106 54-55 0.7093421750304714 1106 56-57 0.5023659183059337 1106 58-59 0.5813964519777954 1106 60-61 0.5376306461500313 1106 62-63 0.5955822168830345 1106 64-65 0.6993074270791446 1106 66-67 0.6498362115418175 1106 68-69 0.6693193293289887 1106 70-71 0.70619265761243 1106 72-73 0.6915825572838905 1106 74-75 0.6462403125202769 1106 76-77 0.7168723990312031 1106 78-79 0.8499731017416323 1106 80-81 0.8816934370217453 1106 82-83 0.9066639224406003 1106 84-85 0.9451618955095533 1106 86-87 1.0056301372694811 1106 88-89 1.0322948645621928 1106 90-91 0.9524788961401391 1106 92-93 1.0909014608745764 1106 94-95 1.1821651312031136 1106 96-97 1.3681910546861822 1106 98-99 1.4842746755034284 1106 100-101 1.6752313797291762 1107 1 0.07234551539366763 1107 2 0.0894143406830814 1107 3 0.14023084692312082 1107 4 0.07479931047664934 1107 5 0.15692002422382245 1107 6 0.23924202342927003 1107 7 0.20914679839409445 1107 8 0.21259068875660603 1107 9 0.195817537249539 1107 10-11 0.1867371935761426 1107 12-13 0.2120803166703098 1107 14-15 0.2471611384684067 1107 16-17 0.26175925740177775 1107 18-19 0.23163523174395806 1107 20-21 0.2423580786663777 1107 22-23 0.353216989380158 1107 24-25 0.4045800104806574 1107 26-27 0.3967721637757933 1107 28-29 0.5026623639637862 1107 30-31 0.5607492968734817 1107 32-33 0.5487311781228357 1107 34-35 0.4595218755665016 1107 36-37 0.356376316658924 1107 38-39 0.44397337848826623 1107 40-41 0.3971389703255568 1107 42-43 0.35964289966737084 1107 44-45 0.3507038352588765 1107 46-47 0.5094402837877112 1107 48-49 0.5029002627318206 1107 50-51 0.37106883876091246 1107 52-53 0.41910136009238386 1107 54-55 0.510085209832134 1107 56-57 0.2860552297068786 1107 58-59 0.2608795707457503 1107 60-61 0.32115488978183393 1107 62-63 0.3914575568752987 1107 64-65 0.44909612173605495 1107 66-67 0.5051579021578654 1107 68-69 0.47311430291212275 1107 70-71 0.5108378879247901 1107 72-73 0.49820239248981224 1107 74-75 0.5464393649855239 1107 76-77 0.5565914736710447 1107 78-79 0.5516256075898838 1107 80-81 0.6322388301034678 1107 82-83 0.633259124025539 1107 84-85 0.8048033886677537 1107 86-87 0.7198726098336579 1107 88-89 0.7600139863325488 1107 90-91 0.8568021859179034 1107 92-93 0.9008751290755228 1107 94-95 0.8526618498729164 1107 96-97 0.956723734786312 1107 98-99 1.1286975818649196 1107 100-101 1.4244594272904187 1108 1 0.09429889595426744 1108 2 0.09144209477655707 1108 3 -0.06983320175524099 1108 4 0.06633401909611081 1108 5 0.14215808192491863 1108 6 0.1574236366780255 1108 7 0.1113730278328049 1108 8 0.07802074432042616 1108 9 0.07540645316392869 1108 10-11 0.06700552064253174 1108 12-13 0.10744777523615312 1108 14-15 0.09315418380958107 1108 16-17 0.13319125927598208 1108 18-19 -0.03829239760473513 1108 20-21 -0.02768710756819104 1108 22-23 0.16350006133369277 1108 24-25 0.23290416594980456 1108 26-27 0.12926351975788464 1108 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2315 86-87 -1.65900641758585 2315 88-89 -1.845619481563105 2315 90-91 -1.9045015529986706 2315 92-93 -1.8343902378646106 2315 94-95 -1.9889801357157424 2315 96-97 -2.0067629323915064 2315 98-99 -2.0598404590227943 2315 100-101 -2.089508356034397 2316 1 -1.2989393469157413 2316 2 -1.121053196442169 2316 3 -2.3068807710884833 2316 4 -1.384294668277846 2316 5 -1.3047625731896346 2316 6 -2.7429573138805807 2316 7 -6.850255642617821 2316 8 -7.797688898089103 2316 9 -4.385488285782994 2316 10-11 -2.2919367990494948 2316 12-13 -2.150542668647759 2316 14-15 -2.1714570112829676 2316 16-17 -2.161906942335982 2316 18-19 -1.7270572368703583 2316 20-21 -2.7284871145197584 2316 22-23 -3.2565792765401795 2316 24-25 -2.033760502807951 2316 26-27 -2.4132907121070843 2316 28-29 -3.1678443647002226 2316 30-31 -3.738872311546512 2316 32-33 -3.68654779186123 2316 34-35 -2.843327490904798 2316 36-37 -3.852622146766212 2316 38-39 -3.324009474486644 2316 40-41 -4.075484670358897 2316 42-43 -3.4844620560812487 2316 44-45 -3.1165986468195186 2316 46-47 -2.8000646662716413 2316 48-49 -2.8696470442702378 2316 50-51 -2.4126216692549107 2316 52-53 -2.7745227107450177 2316 54-55 -4.283637354558401 2316 56-57 -2.9966363553415256 2316 58-59 -2.5338142167737807 2316 60-61 -3.325286062099341 2316 62-63 -3.001085065121469 2316 64-65 -4.483009452603003 2316 66-67 -3.4496873928693788 2316 68-69 -3.3543112638894357 2316 70-71 -3.751772925180589 2316 72-73 -3.3705362456651144 2316 74-75 -3.1561504559642906 2316 76-77 -3.6648125097307407 2316 78-79 -4.679759040145967 2316 80-81 -3.694390552973587 2316 82-83 -3.360224145193474 2316 84-85 -3.416639413798368 2316 86-87 -4.52030487029986 2316 88-89 -4.962386444731944 2316 90-91 -4.3006784678077175 2316 92-93 -4.472547178496253 2316 94-95 -4.627413283090238 2316 96-97 -4.74400697516408 2316 98-99 -4.816527814667111 2316 100-101 -4.99588047369425 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87768.0 3 15821.0 4 14025.0 5 13509.0 6 15360.0 7 18896.0 8 23738.0 9 28494.0 10 33470.0 11 40424.0 12 42657.0 13 48456.0 14 55656.0 15 66987.0 16 84323.0 17 112668.0 18 162725.0 19 240689.0 20 311379.0 21 224528.0 22 131762.0 23 117530.0 24 124259.0 25 131793.0 26 144283.0 27 162538.0 28 187857.0 29 219395.0 30 257258.0 31 307482.0 32 372335.0 33 459665.0 34 587730.0 35 798921.0 36 1118086.0 37 1272099.0 38 984240.0 39 164692.0 40 189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.843094771439207 48.48549896878309 23.628483695783707 17.042922563993994 2 16.018474562321945 49.95514893399007 18.59087314326452 15.435503360423471 3 44.009586545089725 20.789694213322974 19.35493892158235 15.845780320004954 4 16.15358458116219 19.3458067174045 20.265430881053092 44.235177820380216 5 16.18008149257141 47.39519798962901 21.2837434265769 15.140977091222677 6 44.27302673953448 20.290981551057346 19.287690467715713 16.14830124169246 7 16.97022483232047 19.767955527715994 48.078261820184935 15.183557819778603 8 43.81035784058614 20.517014661845685 20.072881443678188 15.599746053889978 9 43.34439741410897 20.173256617142048 20.740367045866456 15.741978922882529 10-11 15.768855857745715 34.013872353641496 34.108172756026924 16.109099032585863 12-13 15.644448250929916 20.532978066198957 34.00850324541932 29.814070437451807 14-15 15.858045760130315 47.99622212649556 20.071919953717018 16.073812159657113 16-17 29.696669983311807 20.284280600273725 20.20974461687137 29.809304799543096 18-19 15.981742199601237 34.06505123143891 20.211825873196364 29.741380695763485 20-21 29.834379677730354 34.03561685654777 20.269226993669857 15.860776472052022 22-23 29.709140602777406 20.21419646692507 34.08534979790597 15.991313132391552 24-25 15.926229484533577 33.80049241858292 20.627459212819858 29.645818884063647 26-27 16.163170816026746 25.960823095082354 41.77546825356493 16.10053783532597 28-29 27.853840364144656 20.119933385834784 35.95976394610166 16.066462303918904 30-31 24.99326540680483 21.52556264217399 36.531815765059044 16.949356185962134 32-33 18.837692096008183 23.47402815995496 38.56161158558095 19.126668158455907 34-35 18.374885993354027 22.791049392838005 39.43869452298508 19.39537009082289 36-37 18.592838881952506 22.772261263699733 39.21256931064422 19.42233054370354 38-39 19.125533214941896 23.167445329756422 38.48696683748828 19.220054617813396 40-41 19.17704705427086 23.090413960444707 38.457380490449346 19.275158494835082 42-43 19.521164693543813 23.050322176930568 38.477796662110386 18.95071646741523 44-45 19.226888844804197 22.94258026836684 38.61348483942296 19.217046047406004 46-47 19.401599176586487 22.894493934155896 38.42693528702308 19.27697160223454 48-49 19.32393071800319 23.227719727337266 38.15585360819812 19.292495946461425 50-51 19.588387585467068 23.456832019065725 37.44748381216716 19.507296583300047 52-53 19.99598998334661 24.116824062452984 36.34833351181048 19.538852442389917 54-55 20.52383312725708 24.861859915354405 34.80339236010872 19.810914597279794 56-57 20.850441742007938 25.698143451467097 33.4979382758428 19.953476530682167 58-59 21.10846055563835 26.400790787179023 32.54169096070162 19.949057696481002 60-61 20.857607070295145 26.793385128320296 32.1824229920546 20.16658480932996 62-63 20.596235191682045 26.561904537785686 32.29617093487445 20.545689335657823 64-65 20.538902549341724 26.179225177517345 32.77436688608184 20.507505387059087 66-67 20.314337801748767 25.927928349936245 33.11482017492019 20.6429136733948 68-69 20.460729604465104 25.765262340138978 33.2860120899023 20.487995965493617 70-71 20.200383632674818 25.778859632427736 33.77752573157283 20.24323100332462 72-73 20.341480291890015 25.535125520419033 33.51670145023401 20.606692737456946 74-75 20.597031623906997 25.76401660075232 32.88384446433062 20.75510731101006 76-77 20.65171396216422 26.031150490331793 32.67199031618744 20.64514523131655 78-79 20.699002892004277 26.211529953819657 32.095495512964995 20.99397164121107 80-81 20.80902176218569 26.337750568645372 31.94234596625375 20.910881702915187 82-83 21.186703679682587 26.614769796803863 31.458066136728704 20.740460386784854 84-85 21.072409275425727 26.827575320292496 31.261562743687787 20.838452660593994 86-87 21.326204547343732 26.75955158219894 31.139475646062344 20.774768224394975 88-89 21.115174725145017 26.65803432380317 31.150703613834906 21.076087337216904 90-91 21.179007750568275 26.587177457495297 31.178600878005934 21.055213913930494 92-93 21.182391120974565 26.8049136948719 31.13241136331291 20.880283820840628 94-95 21.05063403936114 26.863587471243655 30.644598513635614 21.441179975759596 96-97 21.044847626274375 26.80952155586343 30.918005629446526 21.22762518841567 98-99 21.017956880938552 26.987468606161215 30.599099146937192 21.395475365963044 100-101 21.533269335201435 26.822193236456105 30.174972156020385 21.469565272322072 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8650.0 1 5250.5 2 1415.5 3 915.0 4 765.0 5 677.0 6 778.5 7 906.0 8 870.5 9 894.5 10 1193.0 11 6747.5 12 134103.5 13 153345.5 14 45655.5 15 41962.0 16 45740.0 17 50472.0 18 54876.5 19 58694.0 20 62202.5 21 65890.5 22 70036.0 23 75485.5 24 81286.0 25 88041.5 26 97611.5 27 108815.5 28 119531.0 29 129576.5 30 139810.5 31 152458.5 32 167647.5 33 183818.5 34 206898.5 35 222173.0 36 228788.0 37 238613.5 38 246094.0 39 253914.5 40 263563.5 41 268588.5 42 268491.0 43 270795.0 44 278260.5 45 283238.0 46 280970.0 47 280958.0 48 279461.0 49 274788.0 50 268723.0 51 261196.0 52 259879.0 53 258580.0 54 252295.5 55 241148.0 56 225922.5 57 209235.5 58 202096.5 59 176276.5 60 131080.0 61 103266.0 62 80911.5 63 59797.0 64 43466.0 65 31331.0 66 21641.5 67 13978.0 68 9096.5 69 6152.5 70 4288.0 71 3144.0 72 2505.5 73 2172.0 74 1925.5 75 1705.0 76 1577.0 77 1512.0 78 1392.5 79 1295.0 80 1229.5 81 1137.0 82 1004.5 83 912.0 84 838.5 85 742.5 86 632.5 87 524.0 88 433.0 89 323.0 90 243.0 91 197.0 92 156.5 93 118.0 94 75.5 95 50.0 96 32.5 97 17.5 98 17.5 99 13.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12009337861797773 2 0.10880161747672804 3 0.10430451299135086 4 0.09165164274435747 5 0.09650808003365097 6 0.08694764967490726 7 0.09684563509187541 8 0.09268608566472267 9 0.08764453753704803 10-11 0.07798610732269076 12-13 0.06933489784658384 14-15 0.0772674417148581 16-17 0.06795201099514825 18-19 0.060787132662513434 20-21 0.05688347174724051 22-23 0.049479038211994814 24-25 0.04683848654685204 26-27 0.04375693553144832 28-29 0.04636482057805324 30-31 0.03782794426682878 32-33 0.04957703806760836 34-35 0.0598234674156469 36-37 0.050431814586015394 38-39 0.052413589443978226 40-41 0.05269125570154993 42-43 0.048063484742021365 44-45 0.04775859630233478 46-47 0.0472359304057292 48-49 0.047491818917609015 50-51 0.04892915013327436 52-53 0.04545015525899348 54-55 0.0461361542482883 56-57 0.046103487629750445 58-59 0.04877126147700809 60-61 0.044628045359124284 62-63 0.0532792548352312 64-65 0.049146927590193346 66-67 0.04433949022870662 68-69 0.042221604460169426 70-71 0.05002348185429229 72-73 0.04426326811878497 74-75 0.045989154464867975 76-77 0.04172071630925575 78-79 0.046168820866826145 80-81 0.04808526248771327 82-83 0.038034832850901826 84-85 0.048139706851943014 86-87 0.05072036971643306 88-89 0.04633759839593836 90-91 0.04255915951839387 92-93 0.039967607781057866 94-95 0.04326149181695761 96-97 0.031093176411608976 98-99 0.03206228609489849 100-101 0.0236179652028646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9183687.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.79685698484835 #Duplication Level Percentage of deduplicated Percentage of total 1 91.68425498606257 41.98850713359077 2 4.35133875442452 3.985552772580158 3 1.3613588014353881 1.8703786300320306 4 0.6705459329967992 1.2283558478090448 5 0.38051197208483983 0.8713126183296008 6 0.24518194581709413 0.6737137504711378 7 0.16667631402224634 0.5343275921226942 8 0.1254536301481232 0.4596305566498925 9 0.10658390472616087 0.43930870574676223 >10 0.8514667831623252 8.024657172650663 >50 0.03504808175714219 1.0119348308495328 >100 0.013143328857068075 1.3543038239730663 >500 0.0038663003984440026 1.2476870225477017 >1k 0.0038077200893726405 3.3923125753104486 >5k 5.858030906727139E-4 1.946367073467722 >10k+ 1.7574092720181418E-4 30.97164989386877 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1120002 12.195559365209203 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 22935 0.2497362987218532 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 11518 0.12541803743964705 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC 9703 0.1056547332242486 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0888872845949562E-5 0.0 0.0 0.0 0.0 2 7.622210992164694E-5 0.0 0.0 1.0888872845949562E-5 0.0 3 1.1977760130544519E-4 0.0 0.0 2.1777745691899123E-5 0.0 4 1.4155534699734432E-4 0.0 0.0 2.1777745691899123E-5 0.0 5 1.5244421984329388E-4 0.0 0.0 3.266661853784869E-5 0.0 6 1.6333309268924345E-4 0.0 0.0 3.266661853784869E-5 0.0 7 1.9599971122709212E-4 1.0888872845949562E-5 0.0 3.266661853784869E-5 0.0 8 1.9599971122709212E-4 1.0888872845949562E-5 0.0 4.355549138379825E-5 0.0 9 2.0688858407304168E-4 2.1777745691899123E-5 0.0 4.355549138379825E-5 0.0 10-11 2.667773847257643E-4 2.1777745691899123E-5 0.0 4.355549138379825E-5 0.0 12-13 2.8855513041766345E-4 3.266661853784869E-5 0.0 4.355549138379825E-5 0.0 14-15 3.429994946474112E-4 3.266661853784869E-5 0.0 5.9888800652722595E-5 1.0888872845949562E-5 16-17 3.5933280391633554E-4 3.266661853784869E-5 0.0 6.533323707569738E-5 2.1777745691899123E-5 18-19 4.192216045690581E-4 3.266661853784869E-5 0.0 7.077767349867216E-5 2.1777745691899123E-5 20-21 4.7911040522178076E-4 3.266661853784869E-5 0.0 8.166654634462171E-5 2.1777745691899123E-5 22-23 5.22665896605579E-4 3.266661853784869E-5 0.0 9.255541919057128E-5 2.1777745691899123E-5 24-25 5.771102608353269E-4 3.8111054960823464E-5 0.0 1.0888872845949562E-4 2.1777745691899123E-5 26-27 6.859989892948224E-4 4.355549138379825E-5 0.0 1.1433316488247041E-4 2.1777745691899123E-5 28-29 7.023322985637468E-4 5.444436422974781E-5 0.0 1.1977760130544519E-4 2.1777745691899123E-5 30-31 7.567766627934946E-4 5.9888800652722595E-5 0.0 1.1977760130544519E-4 2.1777745691899123E-5 32-33 8.547765184070406E-4 6.533323707569738E-5 0.0 1.1977760130544519E-4 2.1777745691899123E-5 34-35 9.47331937597612E-4 6.533323707569738E-5 0.0 1.3066647415139475E-4 2.7222182114873906E-5 36-37 9.745541197124858E-4 6.533323707569738E-5 0.0 1.4155534699734432E-4 3.266661853784869E-5 38-39 0.0010235540475192588 6.533323707569738E-5 0.0 1.5788865626626867E-4 3.266661853784869E-5 40-41 0.0011161094667098302 6.533323707569738E-5 0.0 1.74221965535193E-4 3.266661853784869E-5 42-43 0.0012086648859004016 6.533323707569738E-5 0.0 1.7966640195816777E-4 3.266661853784869E-5 44-45 0.0013338869236288215 8.166654634462171E-5 0.0 1.9055527480411734E-4 3.266661853784869E-5 46-47 0.0014318867792423675 9.255541919057128E-5 0.0 2.1233302049601647E-4 4.355549138379825E-5 48-49 0.0015353310712788882 9.799985561354606E-5 0.0 2.2866632976494081E-4 4.355549138379825E-5 50-51 0.0016061087447775606 9.799985561354606E-5 0.0 2.5044407545683994E-4 4.355549138379825E-5 52-53 0.0017095530368140812 9.799985561354606E-5 0.0 2.613329483027895E-4 4.355549138379825E-5 54-55 0.0018347750745425014 1.0344429203652084E-4 0.0 2.613329483027895E-4 4.355549138379825E-5 56-57 0.0019545526758479466 1.0888872845949562E-4 0.0 2.8311069399468864E-4 4.355549138379825E-5 58-59 0.002155996823498013 1.0888872845949562E-4 0.0 2.9944400326361296E-4 4.355549138379825E-5 60-61 0.0022757744248034584 1.0888872845949562E-4 0.0 3.103328761095626E-4 4.355549138379825E-5 62-63 0.002439107517492702 1.1433316488247041E-4 0.0 3.266661853784869E-4 4.355549138379825E-5 64-65 0.0026568849744116932 1.1977760130544519E-4 0.0 3.429994946474112E-4 4.355549138379825E-5 66-67 0.002961773414098281 1.1977760130544519E-4 0.0 3.5388836749336073E-4 4.899992780677303E-5 68-69 0.0031958841802861965 1.1977760130544519E-4 0.0 3.6477724033931035E-4 5.444436422974781E-5 70-71 0.003440883819320062 1.1977760130544519E-4 0.0 3.9199942245418424E-4 3.702216767622851E-4 72-73 0.0037239945133147505 1.1977760130544519E-4 0.0 4.355549138379825E-4 3.702216767622851E-4 74-75 0.0039689941523486155 1.1977760130544519E-4 0.0 4.355549138379825E-4 3.8111054960823467E-4 76-77 0.004306549210573052 1.1977760130544519E-4 0.0 4.5733265952988163E-4 3.8111054960823467E-4 78-79 0.004627770959528564 1.3066647415139475E-4 0.0 4.627770959528564E-4 3.8111054960823467E-4 80-81 0.0050088815091367984 1.3066647415139475E-4 0.0 4.7911040522178076E-4 3.8111054960823467E-4 82-83 0.005596880642818075 1.3066647415139475E-4 0.0 5.008881509136799E-4 3.8111054960823467E-4 84-85 0.00647887934333999 1.3066647415139475E-4 1.0888872845949562E-5 5.22665896605579E-4 3.9199942245418424E-4 86-87 0.007682099792817416 1.3066647415139475E-4 1.0888872845949562E-5 5.335547694515286E-4 3.9199942245418424E-4 88-89 0.009451541630284221 1.3066647415139475E-4 1.0888872845949562E-5 5.607769515664025E-4 3.9199942245418424E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 258290 0.0 91.36763 1 AGTGGTA 257165 0.0 91.25787 5 TGGTATC 257570 0.0 91.07159 7 GGTATCA 258660 0.0 91.00134 8 GTATCAA 259250 0.0 90.95208 9 GTGGTAT 258280 0.0 90.93128 6 AGCAGTG 260380 0.0 90.474945 2 GCAGTGG 260010 0.0 90.45998 3 CAGTGGT 261205 0.0 89.77831 4 CAACGCA 257960 0.0 46.086494 12-13 ATCAACG 258100 0.0 46.08543 10-11 TCAACGC 257870 0.0 45.985573 12-13 ACGCAGA 259155 0.0 45.87415 14-15 AACGCAG 259285 0.0 45.746696 14-15 CGCAGAG 259475 0.0 45.645103 16-17 TATCAAC 259650 0.0 45.616333 10-11 AGAGTAC 253490 0.0 45.58579 18-19 CAGAGTA 257915 0.0 45.320305 18-19 GAGTACT 141995 0.0 45.231766 20-21 GCAGAGT 261300 0.0 45.228134 16-17 >>END_MODULE