FastQCFastQC Report
Sat 18 Jun 2016
SRR578594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9183687
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132160.1439073435320694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG5650.028.5690753
CGCGTAG3150.024.86999754-55
CTCGCGT3550.022.73906352-53
ACTCGCG3600.022.42391450-51
CGTACGC3700.021.8132320-21
TCAACGC83050.020.80763410-11
ACGTACG4050.020.51425720-21
AACGCAG92950.018.61697412-13
CGCTAGT4500.018.46393890-91
ATCAACG89050.018.3394159
CAACGCA87900.017.31012212-13
GGTATCA105700.017.202237
CGCAGAG102650.017.04274414-15
GTATCAA108400.016.992748
AGTGGTA108700.016.9897024
TCGCGTA5150.016.59652952-53
CGTAGCG4900.016.4710856-57
TATCAAC114750.016.424759
TGGTATC112600.016.3590856
ACGTGTA11100.016.2525527
Produced by FastQC (version 0.11.5)