##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578594_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9183687 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40666586306785 31.0 31.0 34.0 30.0 34.0 2 31.78109663362874 31.0 31.0 34.0 30.0 34.0 3 31.854485458835867 31.0 31.0 34.0 30.0 34.0 4 35.56170163464848 37.0 35.0 37.0 33.0 37.0 5 35.38614218886162 37.0 35.0 37.0 33.0 37.0 6 35.37789626323284 37.0 35.0 37.0 33.0 37.0 7 35.3735592251783 37.0 35.0 37.0 33.0 37.0 8 35.33091077690256 37.0 35.0 37.0 32.0 37.0 9 36.87455430482333 39.0 37.0 39.0 33.0 39.0 10-11 36.87146001382669 39.0 37.0 39.0 33.0 39.0 12-13 36.86300578406036 39.0 37.0 39.0 32.5 39.0 14-15 38.038334603520354 40.0 37.0 41.0 33.0 41.0 16-17 37.97314264956983 40.0 37.0 41.0 33.0 41.0 18-19 37.91467925681701 40.0 37.0 41.0 33.0 41.0 20-21 37.87350151415221 40.0 37.0 41.0 32.0 41.0 22-23 37.6720458787413 40.0 37.0 41.0 32.0 41.0 24-25 37.68090054680653 39.5 37.0 41.0 32.0 41.0 26-27 37.5129469242582 39.0 36.0 41.0 32.0 41.0 28-29 37.36196682225777 39.0 36.0 41.0 31.5 41.0 30-31 37.219740666248754 39.0 36.0 41.0 31.0 41.0 32-33 37.07867896630188 39.0 36.0 41.0 31.0 41.0 34-35 36.92989226440318 39.0 36.0 40.0 30.0 41.0 36-37 36.77236920204271 39.0 35.0 40.0 30.0 41.0 38-39 36.596544013314045 38.5 35.0 40.0 30.0 41.0 40-41 36.431613522978296 38.0 35.0 40.0 30.0 41.0 42-43 36.51560576922972 39.0 35.0 40.0 30.0 41.0 44-45 36.44451672841202 38.5 35.0 40.0 30.0 41.0 46-47 36.29129498860316 38.0 35.0 40.0 30.0 41.0 48-49 36.13843677381426 38.0 35.0 40.0 29.0 41.0 50-51 35.94791857562218 38.0 34.5 40.0 28.5 41.0 52-53 35.987198333305564 38.0 35.0 40.0 29.0 41.0 54-55 35.97189342363258 38.0 35.0 40.0 28.5 41.0 56-57 35.80951724508904 38.0 34.0 40.0 28.0 41.0 58-59 35.584696647435834 38.0 34.0 40.0 28.0 41.0 60-61 35.32586612544613 38.0 34.0 40.0 28.0 41.0 62-63 35.04413347275446 37.5 34.0 40.0 27.5 41.0 64-65 34.72525849367471 37.0 33.0 40.0 26.5 41.0 66-67 34.387320909347196 36.5 33.0 39.0 26.0 41.0 68-69 34.012732740129316 36.0 33.0 39.0 26.0 41.0 70-71 33.61845051992735 35.5 32.0 39.0 26.0 40.0 72-73 33.22694452674617 35.0 32.0 38.0 26.0 40.0 74-75 32.814070209492115 35.0 31.5 37.5 25.0 39.5 76-77 31.43280933899424 34.0 29.5 36.0 23.0 39.0 78-79 32.08513546901152 34.5 31.0 36.0 24.5 39.0 80-81 31.95503755735578 35.0 31.0 36.0 24.0 38.5 82-83 31.583082589813873 34.0 31.0 36.0 23.0 37.0 84-85 31.29533116710097 34.0 31.0 35.0 23.0 37.0 86-87 30.969678681340078 34.0 31.0 35.0 21.5 36.5 88-89 30.655305597849754 34.0 31.0 35.0 20.0 36.0 90-91 30.3059016493049 34.0 30.0 35.0 19.5 36.0 92-93 29.932046246785198 34.0 30.0 35.0 17.5 35.5 94-95 29.546030532181682 34.0 30.0 35.0 10.0 35.0 96-97 29.076377984136435 34.0 29.0 35.0 2.0 35.0 98-99 28.257440557370913 34.0 29.0 35.0 2.0 35.0 100-101 27.19485436513679 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.31989990151375736 1101 2 0.25048881995714467 1101 3 0.3614211973715449 1101 4 0.31799357053917277 1101 5 0.2795946232635913 1101 6 0.17154825439232013 1101 7 0.21429722264588236 1101 8 0.17161886230750412 1101 9 0.3077197580696236 1101 10-11 0.3249575943403116 1101 12-13 0.2833877972147718 1101 14-15 0.23628825399821096 1101 16-17 0.32017684275209035 1101 18-19 0.3742655679138025 1101 20-21 0.28329173409318287 1101 22-23 0.5174970522317395 1101 24-25 0.4502306502476827 1101 26-27 0.3547721530016119 1101 28-29 0.3245823501948095 1101 30-31 0.36146734555484983 1101 32-33 0.4229999220689322 1101 34-35 0.44157431839254 1101 36-37 0.4047551458041667 1101 38-39 0.3305195162947854 1101 40-41 0.3260722921553807 1101 42-43 0.29595707075225164 1101 44-45 0.2623978672273566 1101 46-47 0.20338525413023945 1101 48-49 0.3126996058047311 1101 50-51 0.3856216386704574 1101 52-53 0.4111940981007507 1101 54-55 0.4447759080682303 1101 56-57 0.5172890186186763 1101 58-59 0.49473619719277195 1101 60-61 0.43634425898677875 1101 62-63 0.32612016986005443 1101 64-65 0.39132302100065885 1101 66-67 0.4029137611077118 1101 68-69 0.23512071287278502 1101 70-71 0.325164269235124 1101 72-73 0.4712937985010015 1101 74-75 0.43344563497358735 1101 76-77 0.33233315795158447 1101 78-79 0.3630985324598086 1101 80-81 0.38923853948946885 1101 82-83 0.5674208379859564 1101 84-85 0.5046167916232847 1101 86-87 0.5060483658564436 1101 88-89 0.5481256087662771 1101 90-91 0.448978162480568 1101 92-93 0.48287795365735775 1101 94-95 0.4406232950012985 1101 96-97 0.4927175548483156 1101 98-99 0.8423699583212496 1101 100-101 1.0177757222152835 1102 1 0.2584686303719046 1102 2 0.318289659518026 1102 3 0.29169099568798273 1102 4 0.30682852930213755 1102 5 0.29269886812192425 1102 6 0.2229207497888126 1102 7 0.2645986040680981 1102 8 0.15671258116205422 1102 9 0.25012104436507343 1102 10-11 0.26779549582714424 1102 12-13 0.28015920096908076 1102 14-15 0.318750495062595 1102 16-17 0.24038774954082953 1102 18-19 0.3250603383961561 1102 20-21 0.290942648264199 1102 22-23 0.5058132192899123 1102 24-25 0.3930454473692393 1102 26-27 0.4614118589793108 1102 28-29 0.42540933711232753 1102 30-31 0.4422982424418862 1102 32-33 0.408515453343135 1102 34-35 0.42541944631813067 1102 36-37 0.46171388422130377 1102 38-39 0.4675091419658557 1102 40-41 0.42954793317270656 1102 42-43 0.42194360572963063 1102 44-45 0.5329420303155743 1102 46-47 0.5021538858238443 1102 48-49 0.3757750845415373 1102 50-51 0.37467350406284083 1102 52-53 0.5184587392403444 1102 54-55 0.44991166517355907 1102 56-57 0.4756695814968239 1102 58-59 0.5552737097435738 1102 60-61 0.6533712615117651 1102 62-63 0.49931345596654353 1102 64-65 0.41917168908696567 1102 66-67 0.4489520716303801 1102 68-69 0.5105443546533479 1102 70-71 0.5159271370524507 1102 72-73 0.5657983056757985 1102 74-75 0.500846614445841 1102 76-77 0.3461311937273983 1102 78-79 0.4007394279852363 1102 80-81 0.5109180757487906 1102 82-83 0.5293108503237249 1102 84-85 0.49537647542635455 1102 86-87 0.49100909711915364 1102 88-89 0.46171209284574033 1102 90-91 0.5722029240152686 1102 92-93 0.6135930855751219 1102 94-95 0.5834743725180367 1102 96-97 0.697466836303132 1102 98-99 1.0016708923605115 1102 100-101 1.084293311404732 1103 1 0.22777422881429743 1103 2 0.25842943810344465 1103 3 0.2562786749328758 1103 4 0.20367768515610152 1103 5 0.23361837969109445 1103 6 0.0400332637916847 1103 7 0.21108888393710146 1103 8 0.06267748852619093 1103 9 0.16618189107981607 1103 10-11 0.14892349170295915 1103 12-13 0.22521078395430294 1103 14-15 0.32454054854842695 1103 16-17 0.2565219076261229 1103 18-19 0.21273784325060774 1103 20-21 0.3714879031619063 1103 22-23 0.2924238788872273 1103 24-25 0.2573561674429854 1103 26-27 0.12946059754536776 1103 28-29 0.25112690962274087 1103 30-31 0.2685619347013599 1103 32-33 0.16181845332642553 1103 34-35 0.18761820627289438 1103 36-37 0.22088881717665032 1103 38-39 0.3098301778900989 1103 40-41 0.23154405144877188 1103 42-43 0.18981557809665617 1103 44-45 0.17951127237673603 1103 46-47 0.2361899501253788 1103 48-49 0.18408255033928356 1103 50-51 0.1331142781404111 1103 52-53 0.0821519979422547 1103 54-55 0.30772456635533274 1103 56-57 0.24181182243924582 1103 58-59 0.1828954196259076 1103 60-61 0.24374087176069992 1103 62-63 0.29896591895236924 1103 64-65 0.20033411247856492 1103 66-67 0.1743961995050327 1103 68-69 0.35811134000376654 1103 70-71 0.27748626305182 1103 72-73 0.23375993315146815 1103 74-75 0.2810556224871732 1103 76-77 0.28966385424159924 1103 78-79 0.39288765730545805 1103 80-81 0.32296721814186213 1103 82-83 0.5972831649731845 1103 84-85 0.37241784190987204 1103 86-87 0.3105348335392222 1103 88-89 0.38944320699882695 1103 90-91 0.39394308189485727 1103 92-93 0.3187131376821277 1103 94-95 0.41421287230644666 1103 96-97 0.47066801583174467 1103 98-99 1.009497992380112 1103 100-101 0.7952266404961215 1104 1 0.2801234372145096 1104 2 0.2472489563724558 1104 3 0.16017608062458422 1104 4 0.18617162093705275 1104 5 0.20029284950368975 1104 6 0.11382803434143085 1104 7 0.14371797635850925 1104 8 0.0845575656402957 1104 9 0.2746845512813394 1104 10-11 0.13923321506605646 1104 12-13 0.21387077123992526 1104 14-15 0.26997401610145744 1104 16-17 0.3339947007971631 1104 18-19 0.20566237564219847 1104 20-21 0.2584121519552909 1104 22-23 0.3775823512717835 1104 24-25 0.2132430816791313 1104 26-27 0.26205679928717274 1104 28-29 0.2489175285632399 1104 30-31 0.27026095086170443 1104 32-33 0.3316732504851885 1104 34-35 0.24927172925342944 1104 36-37 0.19839671052023533 1104 38-39 0.2075459817218004 1104 40-41 0.2608384317013872 1104 42-43 0.19119550432196775 1104 44-45 0.17705179909187763 1104 46-47 0.12671570036708601 1104 48-49 0.21936843215490143 1104 50-51 0.1788095006852899 1104 52-53 0.08729466266345298 1104 54-55 0.2259282430075089 1104 56-57 0.14045382333366518 1104 58-59 0.1633747733128672 1104 60-61 0.13188406144622888 1104 62-63 0.11520762181697108 1104 64-65 0.20307206577771808 1104 66-67 0.22623140713164247 1104 68-69 0.1464708082823023 1104 70-71 0.20868754757828611 1104 72-73 0.2594179075610654 1104 74-75 0.25848682684820545 1104 76-77 0.18684616341901972 1104 78-79 0.22971112245601688 1104 80-81 0.3060831121991896 1104 82-83 0.401992013936443 1104 84-85 0.3746348303374454 1104 86-87 0.4635463584221675 1104 88-89 0.4507869534765767 1104 90-91 0.3925370333100666 1104 92-93 0.37372461725648165 1104 94-95 0.4728833840501814 1104 96-97 0.425283129154959 1104 98-99 0.8652483935149533 1104 100-101 1.0045570494676674 1105 1 0.27706260404803373 1105 2 0.1929898585944514 1105 3 0.10985674992659611 1105 4 0.1849220603605275 1105 5 0.18616919742229499 1105 6 0.09980568064906947 1105 7 0.2366045554252807 1105 8 0.08021877921651566 1105 9 0.2529459213434109 1105 10-11 0.07999628358002298 1105 12-13 0.11993939152304023 1105 14-15 0.138451216191811 1105 16-17 0.14055880162204915 1105 18-19 0.15983824655941703 1105 20-21 0.09827602470389962 1105 22-23 0.25435244795256295 1105 24-25 0.257513445220674 1105 26-27 0.23891800364555849 1105 28-29 0.225519720365142 1105 30-31 0.12269047081451134 1105 32-33 0.22472860403819084 1105 34-35 0.20103176022885094 1105 36-37 0.2182197802038317 1105 38-39 0.23343743798152872 1105 40-41 0.22889673648442255 1105 42-43 0.226178819817477 1105 44-45 0.23638259925893834 1105 46-47 0.2318750754846235 1105 48-49 0.22456019471209032 1105 50-51 0.22659198557531823 1105 52-53 0.22613424662588955 1105 54-55 0.2747639637391899 1105 56-57 0.282131794896209 1105 58-59 0.18571303558080388 1105 60-61 0.13141138092280613 1105 62-63 0.1587905063785442 1105 64-65 0.1879377125253754 1105 66-67 0.26390384212142237 1105 68-69 0.26737234872653914 1105 70-71 0.30303415394637767 1105 72-73 0.3532838275828425 1105 74-75 0.35966668295862547 1105 76-77 0.19826845084730493 1105 78-79 0.32265010080214296 1105 80-81 0.4681944493357548 1105 82-83 0.45410550769929614 1105 84-85 0.32107854380442546 1105 86-87 0.3747983658564422 1105 88-89 0.4050929176570115 1105 90-91 0.6184195054386059 1105 92-93 0.7024069316382473 1105 94-95 0.7453218305226947 1105 96-97 0.7402280710510567 1105 98-99 1.174868919683938 1105 100-101 1.2465031318538387 1106 1 0.13082734292175857 1106 2 0.14335108617192915 1106 3 0.12510463695051044 1106 4 0.08744767197909198 1106 5 0.10166278185199928 1106 6 0.03212288858458123 1106 7 0.06093311394360512 1106 8 0.045421469584205454 1106 9 0.0771507723791629 1106 10-11 -0.04491611009061813 1106 12-13 0.05247443783556349 1106 14-15 -0.0051420497656664566 1106 16-17 -0.014816802683384367 1106 18-19 0.10134024357324023 1106 20-21 0.13495431568264848 1106 22-23 0.18608440563966866 1106 24-25 0.08723649039507819 1106 26-27 0.1127932777259062 1106 28-29 0.13817383350931323 1106 30-31 0.19086087689955633 1106 32-33 0.1754379398482655 1106 34-35 0.13263424742763164 1106 36-37 0.1271565547915614 1106 38-39 0.14828850938209825 1106 40-41 0.18090756099717709 1106 42-43 0.1937459736876761 1106 44-45 0.12102449677529847 1106 46-47 0.12925449579645942 1106 48-49 0.1118312327077362 1106 50-51 0.14309901104121536 1106 52-53 0.2719730702079133 1106 54-55 0.23630147426329273 1106 56-57 0.2337911894813729 1106 58-59 0.24260153422004294 1106 60-61 0.3187196165010846 1106 62-63 0.2636457226063058 1106 64-65 0.1789362432019601 1106 66-67 0.16016541755328007 1106 68-69 0.22796647627228595 1106 70-71 0.26382705928120487 1106 72-73 0.32032369073117195 1106 74-75 0.3830433193119873 1106 76-77 0.11669454102759502 1106 78-79 0.23677769083304412 1106 80-81 0.3605100239695389 1106 82-83 0.5170326155605096 1106 84-85 0.5083344640370768 1106 86-87 0.5506734353419773 1106 88-89 0.5255203101577806 1106 90-91 0.5855691627585067 1106 92-93 0.5483621333693733 1106 94-95 0.5309394205773579 1106 96-97 0.5594305358675591 1106 98-99 1.0462898392544133 1106 100-101 1.0570412712855237 1107 1 0.1791864347737473 1107 2 0.0904249837590072 1107 3 0.017136345855526258 1107 4 -0.021342486577054842 1107 5 0.02339932373798348 1107 6 0.043256744221274346 1107 7 0.03467807634116582 1107 8 0.060275148876677065 1107 9 0.04785827859040381 1107 10-11 0.00874739999377283 1107 12-13 0.020050089449320296 1107 14-15 0.0396707929410951 1107 16-17 0.0941517015556741 1107 18-19 0.024637270556937096 1107 20-21 0.07150990227108167 1107 22-23 0.16623720045294732 1107 24-25 0.14475544589826939 1107 26-27 0.08696715953588097 1107 28-29 0.07262920603431411 1107 30-31 0.062398556533139526 1107 32-33 0.07465719534882709 1107 34-35 0.14488624817622764 1107 36-37 0.21107694838844537 1107 38-39 0.21995743059942185 1107 40-41 0.167191074520197 1107 42-43 0.19600318732513955 1107 44-45 0.18701400040746563 1107 46-47 0.15602769434073593 1107 48-49 0.13210900053456953 1107 50-51 0.1789428158562032 1107 52-53 0.19244095577965936 1107 54-55 0.18017955289791843 1107 56-57 0.26904121846241935 1107 58-59 0.1756201551071257 1107 60-61 0.16542339686526475 1107 62-63 0.2818178658708135 1107 64-65 0.33889994107927635 1107 66-67 0.33047270535789863 1107 68-69 0.32061682077417686 1107 70-71 0.3454107038503196 1107 72-73 0.36004923789165133 1107 74-75 0.4768372023414429 1107 76-77 0.4089533693608125 1107 78-79 0.3861177314942026 1107 80-81 0.3919478334022557 1107 82-83 0.5875684810918571 1107 84-85 0.591945518700765 1107 86-87 0.6239128225480286 1107 88-89 0.6055125706082087 1107 90-91 0.6529238557269643 1107 92-93 0.6015528007237627 1107 94-95 0.623992445685559 1107 96-97 0.6986700416396197 1107 98-99 1.1373226242358 1107 100-101 1.2465157850354274 1108 1 0.084081003189862 1108 2 -0.014924553513015582 1108 3 0.006114212644813932 1108 4 -0.037049972917145624 1108 5 0.004632078135671236 1108 6 0.010500985390038409 1108 7 -0.04337992069183372 1108 8 0.015909768750084652 1108 9 0.0161048700180757 1108 10-11 0.008680472070956569 1108 12-13 -0.13850843913414224 1108 14-15 -0.1591913894522179 1108 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-1.1440923962593708 2316 92-93 -1.1060647569975899 2316 94-95 -1.241717637511396 2316 96-97 -1.4273353053328783 2316 98-99 -1.088800154335388 2316 100-101 -1.4148596624250125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 116.0 3 84.0 4 216.0 5 539.0 6 1031.0 7 2130.0 8 4124.0 9 6493.0 10 8999.0 11 11175.0 12 13911.0 13 16631.0 14 19112.0 15 22153.0 16 25635.0 17 29912.0 18 35021.0 19 40252.0 20 42522.0 21 44803.0 22 49814.0 23 58928.0 24 72707.0 25 91022.0 26 115960.0 27 147836.0 28 185599.0 29 229269.0 30 281057.0 31 341008.0 32 415823.0 33 513789.0 34 660326.0 35 903286.0 36 1189841.0 37 1661413.0 38 1579905.0 39 360422.0 40 823.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.492381779091062 31.79551074597718 27.5273853176939 22.184722157237864 2 22.70388788293852 29.205241859832547 28.50490222499961 19.58596803222932 3 20.729049236978568 28.452777190686053 28.963661326872312 21.85451224546307 4 21.38080776513811 29.12618240539396 28.576845053306926 20.916164776161008 5 22.20728017293383 28.81915513128363 28.308333046591173 20.665231649191366 6 21.85998149759704 28.592724130391417 28.324291198553258 21.223003173458284 7 22.097638754184192 28.93579897097772 28.094577450472137 20.871984824365946 8 21.177344132046315 28.689490397484146 28.584946329290183 21.548219141179352 9 21.000865775976898 28.546303225867568 29.021429234873334 21.43140176328219 10-11 21.137328009170194 29.03173574865069 28.36562372616903 21.465312516010083 12-13 21.200003876438732 29.074444719207005 28.103576482952867 21.621974921401392 14-15 21.379261945665178 29.037493329204274 27.92356163706363 21.659683088066917 16-17 21.57495367328543 28.836482431999528 27.89879793364015 21.68976596107489 18-19 21.60889429364201 28.860361732750878 27.883885620653842 21.64685835295327 20-21 21.659037558557642 28.76958118994922 27.9560734110465 21.615307840446636 22-23 21.704224311529686 28.768330765632815 27.897782561889823 21.62966236094768 24-25 21.59203052107503 28.709313590500194 27.928222074641702 21.770433813783068 26-27 21.810039309954103 28.521570712565193 28.079672645352694 21.588717332128006 28-29 21.814264878856644 28.61874094847537 27.984435257005725 21.582558915662265 30-31 21.735740792545464 28.64358169194739 27.92759546165467 21.69308205385248 32-33 21.77517903641314 28.62857810812478 27.91521456575697 21.68102828970511 34-35 21.784224966520505 28.68397616992845 27.86164128439083 21.67015757916021 36-37 21.823070940456425 28.685433165072393 27.811303835810424 21.680192058660754 38-39 21.83188502440877 28.64218914968274 27.818855906365098 21.707069919543397 40-41 21.791565247379214 28.699537677231255 27.79749267456286 21.711404400826666 42-43 21.8027829982944 28.677473376544853 27.82770714419843 21.692036480962308 44-45 21.794232278815333 28.648642851021716 27.81367862610274 21.743446244060216 46-47 21.846849471195714 28.691412624374014 27.773220992483083 21.68851691194719 48-49 21.84585347218834 28.71161130586899 27.70190980813058 21.74062541381209 50-51 21.855421840252482 28.67319987309137 27.744482199659597 21.726896086996554 52-53 21.840212189343376 28.741016067211444 27.665579619193963 21.753192124251214 54-55 21.864997304846376 28.742223528963372 27.59128948127821 21.80148968491204 56-57 21.88260542399491 28.804533855582086 27.558761287830063 21.754099432592938 58-59 21.859025310471562 28.846356653983328 27.53162835740195 21.762989678143157 60-61 21.831532467925598 28.835773085241968 27.560566227952144 21.772128218880294 62-63 21.81757867312334 28.85250405352234 27.559280569405757 21.770636703948565 64-65 21.83647655884454 28.903025421476276 27.53933555344721 21.721162466231974 66-67 21.797229538407397 28.92121996655776 27.515436219101975 21.766114275932868 68-69 21.800000664239434 28.936817817253687 27.536100188213876 21.727081330293004 70-71 21.845879189982913 28.97759680122306 27.47003052209885 21.706493486695173 72-73 21.80135668712091 28.960255972373833 27.461613644992312 21.776773695512944 74-75 21.82133163333002 29.003758529621265 27.479221864098495 21.695687972950218 76-77 21.781366122946785 29.059851908168277 27.468066980789636 21.6907149880953 78-79 21.823921426493566 29.06076063040304 27.426523948491184 21.688793994612215 80-81 21.794936988664112 29.101546060314696 27.423909735383422 21.679607215637766 82-83 21.815810567027288 29.153058461101555 27.380611577884707 21.650519393986446 84-85 21.789414093388686 29.172798012823183 27.378191013147013 21.659596880641118 86-87 21.769457642737983 29.21258559411486 27.361157306424005 21.656799456723157 88-89 21.775081519661956 29.270796197687332 27.3401963092331 21.61392597341761 90-91 21.766212757565977 29.2539951817336 27.32675395530083 21.65303810539959 92-93 21.76344941688178 29.28893137060821 27.28817632555922 21.65944288695079 94-95 21.76711317585207 29.369983114485144 27.25276037933227 21.61014333033052 96-97 21.73350793447187 29.37794097694607 27.244365443696466 21.64418564488559 98-99 21.772483993867105 29.39026864557058 27.189466079838525 21.647781280723795 100-101 21.797234057091025 29.3845078777079 27.150830536928737 21.667427528272338 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 99400.0 1 57129.5 2 10876.0 3 6144.5 4 4955.0 5 4509.0 6 4516.5 7 4277.5 8 3910.5 9 3606.5 10 3565.5 11 4329.5 12 7739.5 13 7699.0 14 5077.5 15 5537.5 16 6264.0 17 7473.5 18 9301.5 19 11626.0 20 14883.0 21 19755.0 22 25663.0 23 32842.5 24 41149.5 25 51384.5 26 66596.5 27 84228.0 28 97847.5 29 110391.0 30 128058.5 31 150698.5 32 175161.0 33 198825.0 34 221620.5 35 241468.0 36 258667.0 37 273503.0 38 283426.5 39 288672.0 40 294893.5 41 302736.5 42 308095.5 43 313480.0 44 320419.0 45 327341.5 46 330563.0 47 329565.5 48 327093.5 49 322044.0 50 314954.0 51 305622.5 52 300098.0 53 294951.0 54 280169.0 55 257126.5 56 228956.5 57 202093.5 58 179629.5 59 155466.5 60 128954.0 61 104912.0 62 81620.5 63 60526.5 64 44088.0 65 30427.5 66 19942.5 67 13577.5 68 9071.5 69 5796.0 70 3279.0 71 1491.5 72 677.5 73 352.0 74 193.0 75 106.0 76 70.5 77 51.5 78 41.5 79 31.5 80 21.0 81 20.5 82 15.5 83 10.5 84 12.0 85 11.0 86 4.5 87 1.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16130776233989683 2 0.0 3 0.0 4 8.384432091381164E-4 5 6.424434979110242E-4 6 1.6333309268924345E-4 7 1.9599971122709215E-4 8 0.0 9 1.9599971122709215E-4 10-11 2.341107661879156E-4 12-13 0.0 14-15 0.0 16-17 1.0888872845949562E-5 18-19 1.0888872845949562E-5 20-21 1.0888872845949562E-5 22-23 5.281103330285538E-4 24-25 0.0 26-27 8.71109827675965E-5 28-29 0.005406325368013958 30-31 0.003702216767622851 32-33 1.1433316488247041E-4 34-35 8.765542640989397E-4 36-37 3.756661131852599E-4 38-39 0.001121553903132805 40-41 0.0030053289054820793 42-43 0.0011161094667098302 44-45 6.533323707569738E-4 46-47 0.008711098276759649 48-49 0.008438876455610912 50-51 0.007649433174279567 52-53 0.01439508990234532 54-55 0.005847324718274915 56-57 7.45887789947545E-4 58-59 0.001268553686553124 60-61 5.444436422974781E-6 62-63 8.166654634462172E-5 64-65 8.057765906002677E-4 66-67 9.9088742898141E-4 68-69 0.002738551520756315 70-71 0.0019273304937330728 72-73 0.005536991842165353 74-75 0.0019109971844641482 76-77 0.0013992201607045188 78-79 0.0014536645249342665 80-81 2.3955520261089038E-4 82-83 0.002134219077806114 84-85 5.444436422974781E-5 86-87 0.0016932197275451569 88-89 1.4155534699734432E-4 90-91 0.0010181096110962842 92-93 0.0014046645971274936 94-95 8.275543362921667E-4 96-97 9.69109683289511E-4 98-99 0.5265913352665438 100-101 2.1233302049601647E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9183687.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.38344272260397 #Duplication Level Percentage of deduplicated Percentage of total 1 91.28423831798513 64.24898959130437 2 4.712227741539895 6.633256226850773 3 1.3346516231781487 2.8181212822376893 4 0.6814741469281724 1.918579863490177 5 0.41982084794560065 1.4774218302567108 6 0.26637665859302345 1.124910377563242 7 0.17689180022140044 0.871517772228687 8 0.12001711796661722 0.6757774358508326 9 0.08817517238597822 0.5585464975665807 >10 0.807540140161514 12.221373477830575 >50 0.09016724251537071 4.147930124981984 >100 0.01809562308284777 1.7520348672211978 >500 5.3927916062897414E-5 0.027556291161290554 >1k 5.3927916062896546E-5 0.13690846035643722 >5k 1.617837481886896E-4 0.8854451242569912 >10k+ 5.3927916062896546E-5 0.5016307768424381 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13216 0.1439073435320694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.266661853784869E-5 0.0 0.0 0.0 0.0 2 3.266661853784869E-5 0.0 0.0 0.0 1.0888872845949562E-5 3 6.533323707569738E-5 1.0888872845949562E-5 0.0 0.0 1.0888872845949562E-5 4 8.71109827675965E-5 1.0888872845949562E-5 0.0 0.0 2.1777745691899123E-5 5 1.1977760130544519E-4 1.0888872845949562E-5 0.0 0.0 2.1777745691899123E-5 6 1.8511083838114255E-4 1.0888872845949562E-5 0.0 0.0 2.1777745691899123E-5 7 2.1777745691899125E-4 1.0888872845949562E-5 0.0 1.0888872845949562E-5 4.355549138379825E-5 8 2.2866632976494081E-4 1.0888872845949562E-5 0.0 1.0888872845949562E-5 5.444436422974781E-5 9 2.5044407545683994E-4 1.0888872845949562E-5 0.0 2.1777745691899123E-5 5.444436422974781E-5 10-11 3.321106218014617E-4 1.633330926892434E-5 0.0 2.7222182114873906E-5 5.9888800652722595E-5 12-13 3.429994946474112E-4 2.1777745691899123E-5 0.0 4.355549138379825E-5 7.622210992164694E-5 14-15 3.9199942245418424E-4 2.1777745691899123E-5 0.0 4.355549138379825E-5 9.255541919057128E-5 16-17 4.355549138379825E-4 2.1777745691899123E-5 0.0 4.355549138379825E-5 1.0888872845949562E-4 18-19 5.444436422974781E-4 2.1777745691899123E-5 0.0 4.355549138379825E-5 1.0888872845949562E-4 20-21 6.043324429502008E-4 3.266661853784869E-5 0.0 5.9888800652722595E-5 1.3066647415139475E-4 22-23 6.369990614880494E-4 3.266661853784869E-5 0.0 7.622210992164694E-5 1.5244421984329388E-4 24-25 6.587768071799486E-4 3.266661853784869E-5 0.0 1.0344429203652084E-4 1.5244421984329388E-4 26-27 6.96887862140772E-4 3.266661853784869E-5 0.0 1.1977760130544519E-4 1.5788865626626867E-4 28-29 7.513322263705198E-4 3.266661853784869E-5 0.0 1.1977760130544519E-4 1.6333309268924345E-4 30-31 8.438876455610911E-4 3.266661853784869E-5 0.0 1.4155534699734432E-4 1.6333309268924345E-4 32-33 9.418875011746371E-4 3.266661853784869E-5 0.0 1.5244421984329388E-4 2.0688858407304168E-4 34-35 0.0010126651746733093 3.266661853784869E-5 0.0 1.5244421984329388E-4 2.1777745691899125E-4 36-37 0.0011106650302868553 3.266661853784869E-5 0.0 1.5244421984329388E-4 2.2866632976494081E-4 38-39 0.001192331576631477 3.266661853784869E-5 0.0 1.5244421984329388E-4 2.2866632976494081E-4 40-41 0.0013175536143598971 3.266661853784869E-5 0.0 1.5788865626626867E-4 2.3955520261089038E-4 42-43 0.0014101090335504683 3.266661853784869E-5 0.0 1.6333309268924345E-4 2.5044407545683994E-4 44-45 0.0015298866348559135 3.266661853784869E-5 0.0 1.74221965535193E-4 2.7766625757171383E-4 46-47 0.0016442197997383837 3.266661853784869E-5 0.0 1.9055527480411734E-4 2.939995668406382E-4 48-49 0.001763997401043829 3.266661853784869E-5 0.0 2.1233302049601647E-4 3.1577731253253733E-4 50-51 0.0018837750023492743 3.266661853784869E-5 0.0 2.3955520261089038E-4 3.212217489555121E-4 52-53 0.0020035526036547196 3.266661853784869E-5 0.0 2.5044407545683994E-4 3.3755505822443646E-4 54-55 0.002139663514229089 3.266661853784869E-5 0.0 2.5044407545683994E-4 3.5933280391633554E-4 56-57 0.002264885551957509 4.355549138379825E-5 0.0 2.722218211487391E-4 3.6477724033931035E-4 58-59 0.0024227742082237776 4.355549138379825E-5 0.0 2.8311069399468864E-4 3.8111054960823467E-4 60-61 0.002558885118798147 4.355549138379825E-5 0.0 2.8311069399468864E-4 3.9199942245418424E-4 62-63 0.0027385515207563153 4.355549138379825E-5 0.0 3.103328761095626E-4 3.9199942245418424E-4 64-65 0.0029454401048293567 4.355549138379825E-5 0.0 3.1577731253253733E-4 3.97443858877159E-4 66-67 0.00323399523524702 4.355549138379825E-5 0.0 3.266661853784869E-4 4.2466604099203293E-4 68-69 0.0034898837471268344 4.355549138379825E-5 0.0 3.3755505822443646E-4 4.355549138379825E-4 70-71 0.0037729944411215234 4.355549138379825E-5 0.0 3.48443931070386E-4 4.4099935026095725E-4 72-73 0.004039771825847287 4.899992780677303E-5 0.0 3.48443931070386E-4 4.4644378668393206E-4 74-75 0.004252104846343304 6.533323707569738E-5 0.0 3.6477724033931035E-4 4.5188822310690687E-4 76-77 0.004529771103915017 6.533323707569738E-5 0.0 3.8111054960823467E-4 4.5733265952988163E-4 78-79 0.00486188172571648 7.077767349867216E-5 0.0 3.9199942245418424E-4 4.5733265952988163E-4 80-81 0.00523754783890174 8.166654634462171E-5 0.0 4.083327317231086E-4 4.73665968798806E-4 82-83 0.005814658099737066 9.255541919057128E-5 0.0 4.1377716814608337E-4 4.7911040522178076E-4 84-85 0.006734767855219805 1.0344429203652084E-4 0.0 4.2466604099203293E-4 4.7911040522178076E-4 86-87 0.00797065492323508 1.0888872845949562E-4 0.0 4.355549138379825E-4 4.7911040522178076E-4 88-89 0.009778207815662706 1.0888872845949562E-4 0.0 4.5188822310690687E-4 5.008881509136799E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 565 0.0 28.569075 3 CGCGTAG 315 0.0 24.869997 54-55 CTCGCGT 355 0.0 22.739063 52-53 ACTCGCG 360 0.0 22.423914 50-51 CGTACGC 370 0.0 21.81323 20-21 TCAACGC 8305 0.0 20.807634 10-11 ACGTACG 405 0.0 20.514257 20-21 AACGCAG 9295 0.0 18.616974 12-13 CGCTAGT 450 0.0 18.463938 90-91 ATCAACG 8905 0.0 18.339415 9 CAACGCA 8790 0.0 17.310122 12-13 GGTATCA 10570 0.0 17.20223 7 CGCAGAG 10265 0.0 17.042744 14-15 GTATCAA 10840 0.0 16.99274 8 AGTGGTA 10870 0.0 16.989702 4 TCGCGTA 515 0.0 16.596529 52-53 CGTAGCG 490 0.0 16.47108 56-57 TATCAAC 11475 0.0 16.42475 9 TGGTATC 11260 0.0 16.359085 6 ACGTGTA 1110 0.0 16.252552 7 >>END_MODULE