##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578593_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8525597
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.072835368596476	31.0	31.0	34.0	28.0	34.0
2	31.424395617104587	33.0	31.0	34.0	28.0	34.0
3	31.549893573435384	34.0	31.0	34.0	28.0	34.0
4	34.940955571791626	37.0	35.0	37.0	32.0	37.0
5	34.97724886597384	37.0	35.0	37.0	32.0	37.0
6	35.017619997755	37.0	35.0	37.0	32.0	37.0
7	34.94921294074773	37.0	35.0	37.0	32.0	37.0
8	34.989139998055265	37.0	35.0	37.0	32.0	37.0
9	36.49946531603593	39.0	37.0	39.0	32.0	39.0
10-11	36.3892010143102	39.0	37.0	39.0	32.0	39.0
12-13	36.45112441979136	39.0	37.0	39.0	32.0	39.0
14-15	37.72298749284067	40.0	38.0	41.0	32.0	41.0
16-17	37.69231052089373	40.0	38.0	41.0	32.0	41.0
18-19	37.62426214844544	40.0	38.0	41.0	32.0	41.0
20-21	37.55180335171836	40.0	38.0	41.0	32.0	41.0
22-23	37.286925126768246	39.5	37.5	41.0	31.5	41.0
24-25	37.246556751392305	40.0	37.0	41.0	31.0	41.0
26-27	37.140921392367005	40.0	37.0	41.0	31.0	41.0
28-29	36.982518057093245	40.0	37.0	41.0	30.0	41.0
30-31	36.73260805079104	40.0	36.5	41.0	30.0	41.0
32-33	36.48260755229224	40.0	36.0	41.0	30.0	41.0
34-35	36.32994076543848	39.0	36.0	41.0	29.0	41.0
36-37	36.04904700515401	39.0	35.5	40.5	28.5	41.0
38-39	35.184744540470305	38.0	35.0	40.0	27.0	41.0
40-41	34.94298334767642	38.0	34.5	40.0	26.0	41.0
42-43	34.644676789203146	38.0	34.0	40.0	25.0	41.0
44-45	34.360730104882975	37.0	33.5	40.0	24.0	41.0
46-47	34.2139053136103	37.0	33.5	40.0	24.0	41.0
48-49	34.23121160899348	38.0	33.5	40.0	23.5	41.0
50-51	33.32748662644974	37.0	32.5	39.5	20.5	40.5
52-53	32.88655005625999	37.5	33.0	39.5	11.5	40.5
54-55	32.45947732457915	38.0	32.5	40.0	2.0	41.0
56-57	31.907872140801402	38.0	31.0	40.0	2.0	41.0
58-59	31.59709226227794	37.5	31.0	40.0	2.0	41.0
60-61	31.33594374681327	37.0	31.0	40.0	2.0	41.0
62-63	31.008395071922823	36.5	30.5	40.0	2.0	41.0
64-65	30.718157684441337	36.0	30.0	40.0	2.0	41.0
66-67	30.384348920081493	35.5	29.5	39.0	2.0	41.0
68-69	30.0523309980521	35.0	29.0	39.0	2.0	41.0
70-71	29.74920248986669	35.0	29.0	38.5	2.0	40.5
72-73	29.42363320715253	35.0	29.0	37.5	2.0	40.0
74-75	29.054219839384857	35.0	28.5	37.0	2.0	39.5
76-77	28.676923915122895	35.0	28.0	36.5	2.0	39.0
78-79	28.331205368961257	35.0	27.0	36.0	2.0	39.0
80-81	28.004803534579455	35.0	27.0	36.0	2.0	37.5
82-83	27.731453058360607	34.0	26.0	35.0	2.0	37.0
84-85	27.45061929387467	34.0	26.0	35.0	2.0	37.0
86-87	27.189861249599296	34.0	26.0	35.0	2.0	36.0
88-89	26.93474885101888	34.0	25.0	35.0	2.0	36.0
90-91	26.69103430528091	34.0	25.0	35.0	2.0	36.0
92-93	26.4058246595517	34.0	24.0	35.0	2.0	35.0
94-95	26.129539432839717	34.0	23.0	35.0	2.0	35.0
96-97	25.860515398511097	34.0	20.0	35.0	2.0	35.0
98-99	25.56055575932102	34.0	18.0	35.0	2.0	35.0
100-101	24.713338256546727	33.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.617503650833342
1101	2	0.6214015316055743
1101	3	0.49139875342293493
1101	4	0.5950591436330797
1101	5	0.5039152579230901
1101	6	0.5217167055566136
1101	7	0.4864623209258738
1101	8	0.5096824087908516
1101	9	0.6435967363910322
1101	10-11	0.7179511395954208
1101	12-13	0.7075397035005082
1101	14-15	0.8802589783260473
1101	16-17	0.7936272288759909
1101	18-19	0.7776110367595805
1101	20-21	0.8363889713835917
1101	22-23	0.8561834227381766
1101	24-25	0.8194516531103062
1101	26-27	0.8789478333536209
1101	28-29	0.9427510482481125
1101	30-31	1.0454304058514836
1101	32-33	1.069796095749048
1101	34-35	1.1511716200049875
1101	36-37	1.0915506903554473
1101	38-39	1.1203465852161543
1101	40-41	1.0996227658967612
1101	42-43	1.1562118993773822
1101	44-45	1.3150337718825327
1101	46-47	1.2236308068653372
1101	48-49	1.1869745827653233
1101	50-51	1.3433234834812566
1101	52-53	1.1280349640528655
1101	54-55	0.9960984898924039
1101	56-57	0.9841521863470177
1101	58-59	1.055673902254231
1101	60-61	0.944377720204951
1101	62-63	0.929703286851332
1101	64-65	0.9803548785380052
1101	66-67	0.9502597389857392
1101	68-69	0.8843418893242152
1101	70-71	1.007093433933882
1101	72-73	0.9420277790358718
1101	74-75	0.9262263835095226
1101	76-77	0.8491974384918706
1101	78-79	0.8812464511325473
1101	80-81	0.8106333870110269
1101	82-83	0.8505062428637657
1101	84-85	0.8337472990639334
1101	86-87	0.8348183286789705
1101	88-89	0.8514301040351384
1101	90-91	0.907472034852173
1101	92-93	0.9773045094265385
1101	94-95	1.0472411360510527
1101	96-97	1.074593824541651
1101	98-99	1.0396400310910145
1101	100-101	1.1927473392638497
1102	1	0.5182336805266416
1102	2	0.46609990330362194
1102	3	0.5028886411099762
1102	4	0.5012335359308153
1102	5	0.559851740073654
1102	6	0.5995000994556605
1102	7	0.5632932599433147
1102	8	0.48862608977316313
1102	9	0.6563142564018776
1102	10-11	0.6286779062240484
1102	12-13	0.6017599754197036
1102	14-15	0.7600879403795133
1102	16-17	0.79026915443022
1102	18-19	0.8503202016962845
1102	20-21	0.8900751121338075
1102	22-23	0.8708834835583588
1102	24-25	0.8947731930175422
1102	26-27	0.9380603419057039
1102	28-29	1.0343509736974497
1102	30-31	1.0082884315735967
1102	32-33	1.0981655100275987
1102	34-35	1.0564517043657347
1102	36-37	1.0905651548230963
1102	38-39	1.15889447911443
1102	40-41	1.1981508564049932
1102	42-43	1.1724118449376135
1102	44-45	1.2513559888850665
1102	46-47	1.281637152028793
1102	48-49	1.2062958845990224
1102	50-51	1.205915615772149
1102	52-53	1.06065368568688
1102	54-55	1.015801523685404
1102	56-57	0.9549610112039062
1102	58-59	1.0020934052987478
1102	60-61	1.0343538973149542
1102	62-63	1.0506938302852653
1102	64-65	1.0584742807762701
1102	66-67	1.027061770603769
1102	68-69	0.9667267937045274
1102	70-71	0.9394952107853634
1102	72-73	0.9866931825841867
1102	74-75	0.8983442324840034
1102	76-77	0.9298175343523631
1102	78-79	0.9266937741203982
1102	80-81	0.9953754873114526
1102	82-83	0.9980327932333282
1102	84-85	1.0133745628987576
1102	86-87	1.014879166004615
1102	88-89	1.053085727657372
1102	90-91	0.9697892147028604
1102	92-93	0.9586649207859033
1102	94-95	1.085693870176211
1102	96-97	1.1526349153706157
1102	98-99	1.2309873060837973
1102	100-101	1.332020237810827
1103	1	0.47671422413316833
1103	2	0.46470890523059794
1103	3	0.36911897035452057
1103	4	0.47015332778548213
1103	5	0.47274862660101036
1103	6	0.5400271577668931
1103	7	0.4220562830249577
1103	8	0.4678144042434198
1103	9	0.6051065153582655
1103	10-11	0.6100543579800757
1103	12-13	0.6106150068211136
1103	14-15	0.6983661880882863
1103	16-17	0.7716119055401833
1103	18-19	0.7541298071096278
1103	20-21	0.766865150771487
1103	22-23	0.8552629128992919
1103	24-25	0.7755200191276757
1103	26-27	0.7937994655691014
1103	28-29	0.8704620493600927
1103	30-31	0.9367738635968763
1103	32-33	0.9777910040020643
1103	34-35	0.9370975066689908
1103	36-37	1.0041383374428392
1103	38-39	0.8767278939213767
1103	40-41	1.010401640257534
1103	42-43	0.8688108241257737
1103	44-45	0.8469432703897155
1103	46-47	1.0805591654175473
1103	48-49	1.1451839039151608
1103	50-51	1.0744773751576915
1103	52-53	0.9120359113480419
1103	54-55	0.8570036540315513
1103	56-57	0.8735923899431768
1103	58-59	0.8174465584664468
1103	60-61	0.8764934560135877
1103	62-63	0.7308060911133332
1103	64-65	0.658301419554089
1103	66-67	0.650106579701589
1103	68-69	0.8262731614789338
1103	70-71	0.7554929213638353
1103	72-73	0.8590796006855861
1103	74-75	0.8319181148131882
1103	76-77	0.8258270201900615
1103	78-79	0.8533330670422998
1103	80-81	0.8919186969307695
1103	82-83	0.9153642750278159
1103	84-85	0.9204900523737294
1103	86-87	0.9259400184156661
1103	88-89	0.9402143727116972
1103	90-91	0.8894535330022499
1103	92-93	0.9430744567318285
1103	94-95	0.8761598428109743
1103	96-97	0.8667912354961977
1103	98-99	0.8977418618130066
1103	100-101	0.9666196285427304
1104	1	0.33924275466441856
1104	2	0.3518397938119442
1104	3	0.36456898637537094
1104	4	0.3495943155491048
1104	5	0.4408392005371482
1104	6	0.4332312765781623
1104	7	0.39394971320036376
1104	8	0.3066982741209685
1104	9	0.4874125207469078
1104	10-11	0.46690170281444665
1104	12-13	0.5016115661345637
1104	14-15	0.5972303510368064
1104	16-17	0.5700953331992622
1104	18-19	0.6393095114065446
1104	20-21	0.6570635156670406
1104	22-23	0.6313835819738358
1104	24-25	0.6469423215385177
1104	26-27	0.6567815758157209
1104	28-29	0.6975315733338547
1104	30-31	0.7550618292511615
1104	32-33	0.7456115602129998
1104	34-35	0.808656284469123
1104	36-37	0.7815730404288885
1104	38-39	0.9014388148162311
1104	40-41	0.7789590151844834
1104	42-43	0.9485433976110755
1104	44-45	0.8946410713832265
1104	46-47	1.0508421100727716
1104	48-49	0.9651961235840858
1104	50-51	0.99909427539734
1104	52-53	0.9421182306457823
1104	54-55	1.0253420449751118
1104	56-57	1.0219083978065342
1104	58-59	1.1015230723986953
1104	60-61	1.086759846598806
1104	62-63	0.9715667944525954
1104	64-65	0.8652527491044104
1104	66-67	0.9608343472090581
1104	68-69	1.0077020313637597
1104	70-71	0.898706066421493
1104	72-73	0.927004550714468
1104	74-75	1.0119325855832315
1104	76-77	0.9846925016258083
1104	78-79	0.9164558130375902
1104	80-81	0.9888535018802962
1104	82-83	1.0091219591249327
1104	84-85	1.0388541890079743
1104	86-87	1.0147269207167682
1104	88-89	1.0207622639607372
1104	90-91	0.9927914066744599
1104	92-93	0.8962951902850094
1104	94-95	0.7822940136385164
1104	96-97	0.9318077736181571
1104	98-99	1.1858522064703543
1104	100-101	1.1328331349969396
1105	1	0.2289961786983099
1105	2	0.18179387285951876
1105	3	0.1759858879950329
1105	4	0.20873813375520456
1105	5	0.13500858803130455
1105	6	0.1783704237383148
1105	7	0.17445290749221698
1105	8	0.18159047988145005
1105	9	0.14450678706758424
1105	10-11	0.16930596648327167
1105	12-13	0.14241847037143174
1105	14-15	0.016612440991089272
1105	16-17	0.028242128156307444
1105	18-19	-0.03691470237087202
1105	20-21	0.009063790661798521
1105	22-23	-0.011464116137197777
1105	24-25	-0.050934454129439644
1105	26-27	-0.3129906511212468
1105	28-29	-0.08024929414786186
1105	30-31	-0.19444171552107292
1105	32-33	-0.23302636892855588
1105	34-35	-0.10920421181311468
1105	36-37	0.0025128089540444876
1105	38-39	0.019971173771999418
1105	40-41	0.004264525376250106
1105	42-43	0.12128315834777936
1105	44-45	0.09234093429100199
1105	46-47	0.1527480646914725
1105	48-49	0.1546794119818813
1105	50-51	0.15142247570845768
1105	52-53	0.06650458345858823
1105	54-55	0.028258486710889485
1105	56-57	0.10544227980198428
1105	58-59	0.07701035980185011
1105	60-61	0.003883098651556338
1105	62-63	0.09945243355045719
1105	64-65	-0.04744513843635545
1105	66-67	-0.04027560727327284
1105	68-69	0.00491264842699124
1105	70-71	0.0161645278065059
1105	72-73	0.04829251132568757
1105	74-75	0.10537060017386679
1105	76-77	0.08961180717729178
1105	78-79	0.08107850727772359
1105	80-81	0.15525417302529476
1105	82-83	0.11298823093676447
1105	84-85	0.055763990941116504
1105	86-87	0.09977039518341968
1105	88-89	0.1872075481205151
1105	90-91	0.22341315897026348
1105	92-93	0.21816895276528925
1105	94-95	0.23653926884615473
1105	96-97	0.24875428016788348
1105	98-99	0.2610116625143597
1105	100-101	0.24746318122202027
1106	1	-0.18521212824286337
1106	2	-0.12014864804409342
1106	3	-0.21824952910072426
1106	4	-0.18239246521920904
1106	5	-0.2081888955607667
1106	6	-0.20971596126299374
1106	7	-0.27153756086682534
1106	8	-0.2913773778369162
1106	9	-0.2284419065216241
1106	10-11	-0.40801884199185423
1106	12-13	-0.40509591855102656
1106	14-15	-0.413564986247799
1106	16-17	-0.41595793480166776
1106	18-19	-0.4747552588778703
1106	20-21	-0.22243833169002158
1106	22-23	-0.2374174148413033
1106	24-25	-0.3583979917149023
1106	26-27	-0.3412706325118009
1106	28-29	-0.37078426500305994
1106	30-31	-0.36396724973739225
1106	32-33	-0.2691600597756363
1106	34-35	-0.2764268278090327
1106	36-37	-0.39918274534635145
1106	38-39	-0.41405113039783004
1106	40-41	-0.39697131962905985
1106	42-43	-0.5191347158340633
1106	44-45	-0.44766247845925733
1106	46-47	-0.44836402355078775
1106	48-49	-0.6268918809554478
1106	50-51	-0.7354972506380193
1106	52-53	-0.777758265882035
1106	54-55	-0.8357163393385321
1106	56-57	-0.8430290719653257
1106	58-59	-0.7820377946596473
1106	60-61	-0.9589607678909182
1106	62-63	-0.8027733903724865
1106	64-65	-0.7977797739878092
1106	66-67	-0.9620453321984712
1106	68-69	-1.0754175467412814
1106	70-71	-0.9755372969465022
1106	72-73	-0.9861010734411515
1106	74-75	-1.0803478773742583
1106	76-77	-1.0968009713810822
1106	78-79	-0.954143250303936
1106	80-81	-0.9742457592581317
1106	82-83	-1.050767858097526
1106	84-85	-1.1044520730642375
1106	86-87	-1.1302963911336406
1106	88-89	-1.2251307524933246
1106	90-91	-1.1792939874029855
1106	92-93	-1.1353078099004499
1106	94-95	-1.1579405475895506
1106	96-97	-1.0902980615885305
1106	98-99	-1.1795379236723917
1106	100-101	-1.3339729835646068
1107	1	-0.47148988583959905
1107	2	-0.5105406373042456
1107	3	-0.43702168554409937
1107	4	-0.34119230642618703
1107	5	-0.3958577833337884
1107	6	-0.4180933098431865
1107	7	-0.3479046561095416
1107	8	-0.31467900324849296
1107	9	-0.39757274682629884
1107	10-11	-0.4241114694673058
1107	12-13	-0.4568895195205016
1107	14-15	-0.6899553452662062
1107	16-17	-0.513707568206371
1107	18-19	-0.5416946608051134
1107	20-21	-0.44608686785938545
1107	22-23	-0.5059775669692854
1107	24-25	-0.4775883933986407
1107	26-27	-0.3625496101894612
1107	28-29	-0.40880548599134414
1107	30-31	-0.39444128857181937
1107	32-33	-0.381732824049287
1107	34-35	-0.44306347483453123
1107	36-37	-0.6037122848524064
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2308	60-61	-2.0840215283886074
2308	62-63	-2.16702771922742
2308	64-65	-2.093471281222861
2308	66-67	-2.2608008135253463
2308	68-69	-2.0807761519894292
2308	70-71	-2.0324866907291756
2308	72-73	-1.980302488513253
2308	74-75	-1.7854551201046824
2308	76-77	-1.6786189210084288
2308	78-79	-1.839375982841716
2308	80-81	-2.4524611178982987
2308	82-83	-2.234686661257875
2308	84-85	-1.9550014575175716
2308	86-87	-1.8412011283348235
2308	88-89	-1.7057350357569838
2308	90-91	-1.9482981368014443
2308	92-93	-1.9343111809498126
2308	94-95	-2.026138343767766
2308	96-97	-1.9665855948406943
2308	98-99	-2.0286493892517576
2308	100-101	-2.2247270537156467
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72688.0
3	12617.0
4	11126.0
5	10751.0
6	12272.0
7	15363.0
8	18866.0
9	21965.0
10	25170.0
11	30418.0
12	30461.0
13	33473.0
14	38194.0
15	44563.0
16	57061.0
17	77217.0
18	121284.0
19	202024.0
20	292382.0
21	206141.0
22	106512.0
23	90279.0
24	93411.0
25	96910.0
26	103854.0
27	114159.0
28	131577.0
29	152152.0
30	179970.0
31	214756.0
32	264452.0
33	333316.0
34	437065.0
35	633977.0
36	1033504.0
37	1452623.0
38	1437426.0
39	315116.0
40	502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.885289719882817	48.2802746753595	23.67943071744403	17.155004887313652
2	16.066761748535853	49.80015431627699	18.629992209047366	15.503091726139798
3	43.909169092989295	20.766054288739006	19.40722752617928	15.917549092092415
4	16.19291515731772	19.3412412147148	20.325880887458453	44.139962740509034
5	16.246179658703262	47.2169531232941	21.351648901213846	15.185218316788793
6	44.14765952126086	20.26939721151119	19.354070235123473	16.228873032104477
7	16.98761539639001	19.752272739268818	48.0667948702159	15.193316994125276
8	43.67074071363152	20.530313480623995	20.173558717325047	15.62538708841944
9	43.17984292122002	20.211965910622542	20.8136715056259	15.79451966253154
10-11	15.766602467208804	33.95344453249541	34.12820070818628	16.15175229210951
12-13	15.680210806805048	20.52423552090442	34.00164214765885	29.79391152463169
14-15	15.865079446077562	47.83809467836976	20.152583345093433	16.144242530459245
16-17	29.63126351465873	20.294312392344583	20.277257604130682	29.797166488866004
18-19	16.032887987388957	34.00128285061441	20.259011022697727	29.706818139298903
20-21	29.76093373522946	33.95181281380541	20.341540159233627	15.9457132917315
22-23	29.612875442192177	20.220035895574526	34.10992129728434	16.057167364948956
24-25	15.951801432005992	33.722758122874104	20.684766201440073	29.640674243679833
26-27	16.17773220457551	26.124552781673945	41.52235217778991	16.175362835960634
28-29	28.14765967713917	20.134593181911754	35.5929509567157	16.124796184233382
30-31	25.290650481842746	21.565448175453724	36.137009529877815	17.00689181282571
32-33	18.963027370464193	23.546523595998252	38.217782796401934	19.27266623713562
34-35	18.468677224771078	22.81999518145437	39.1891096176904	19.522217976084157
36-37	18.722214067006394	22.82162244014601	38.85368461741426	19.602478875433338
38-39	19.259851390832026	23.258850849214095	38.08160513619184	19.399692623762043
40-41	19.379922977743497	23.143954109448927	38.03897046541787	19.437152447389707
42-43	19.75450569251538	23.142655797148624	38.00197830201725	19.100860208318746
44-45	19.43571430288957	23.005734710395746	38.1515328150201	19.407018171694588
46-47	19.61437222281717	22.976268491435285	37.97618462539621	19.433174660351337
48-49	19.506659011895216	23.30288491398323	37.712499522749745	19.477956551371804
50-51	19.760497093278655	23.57340396594067	36.93794697416765	19.728151966613027
52-53	20.274396984766742	24.289825972003236	35.70595638468219	19.72982065854783
54-55	20.861487255346532	25.0958974232778	34.003585056887125	20.039030264488538
56-57	21.19728888246852	25.988341161092105	32.63139536112804	20.182974595311332
58-59	21.411408260330344	26.71784582706091	31.724305219674253	20.14644069293449
60-61	21.112037978713126	27.089024732921807	31.434098245695612	20.364839042669452
62-63	20.81282109259098	26.82270971248153	31.61399141837033	20.75047777655716
64-65	20.76629938055478	26.417931112393212	32.131144499286776	20.68462500776523
66-67	20.562927698971095	26.125791098312256	32.49734419039925	20.813937012317403
68-69	20.690670366723545	25.912310249440228	32.70558554562899	20.69143383820724
70-71	20.400713131050132	25.962253332988055	33.19634903491191	20.4406845010499
72-73	20.578122817410303	25.72493464451212	32.88919844047535	20.807744097602228
74-75	20.833695435168114	25.943412291106387	32.24688494556729	20.976007328158214
76-77	20.901949460569906	26.281915421838146	31.999079881906823	20.817055235685125
78-79	20.941677244683326	26.469551405167053	31.385451180309314	21.203320169840307
80-81	21.069149683081594	26.555787264758695	31.281817151937542	21.09324590022216
82-83	21.475439223107788	26.827873135382184	30.77840180841601	20.918285833094018
84-85	21.309677034075108	27.102751219484695	30.57586407091652	21.011707675523677
86-87	21.630545420039045	26.961144639969977	30.49619448854694	20.91211545144404
88-89	21.36479762974348	26.885674335289856	30.502737077177255	21.246790957789408
90-91	21.42631010646385	26.830660212347503	30.54014373585798	21.202885945330667
92-93	21.461601144710198	27.06477007768331	30.459771802712986	21.013856974893503
94-95	21.31147107657053	27.14759543253244	29.92947814485076	21.611455346046277
96-97	21.305226887310862	27.10687032317146	30.22365365784603	21.36424913167165
98-99	21.2895556392005	27.267057132857897	29.95410171270953	21.489285515232076
100-101	21.613183301363012	27.283472161744665	29.58342668001267	21.519917856879648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6651.0
1	3937.0
2	909.5
3	517.5
4	436.5
5	450.5
6	557.5
7	672.0
8	654.0
9	769.0
10	1290.0
11	5884.0
12	109927.5
13	124848.5
14	36818.5
15	35122.0
16	38855.5
17	43407.5
18	48024.0
19	52226.0
20	55984.5
21	59811.5
22	63860.0
23	68700.5
24	74224.5
25	80973.5
26	90034.5
27	100232.5
28	110092.0
29	119496.0
30	129332.5
31	141069.5
32	154635.5
33	169869.0
34	192064.5
35	205712.5
36	210891.0
37	220338.0
38	228206.5
39	235311.5
40	243804.5
41	248678.0
42	249074.5
43	251563.5
44	258607.5
45	264161.5
46	262485.0
47	262306.5
48	261592.5
49	258096.5
50	253956.0
51	248168.5
52	247076.5
53	246722.0
54	242012.0
55	232606.5
56	217919.5
57	202781.5
58	198671.5
59	171647.0
60	124915.5
61	98148.0
62	76667.0
63	56009.5
64	40412.0
65	28897.0
66	19346.5
67	12094.5
68	7448.5
69	4789.0
70	3159.0
71	2113.5
72	1537.0
73	1265.5
74	1099.0
75	959.0
76	810.5
77	695.5
78	639.5
79	584.5
80	484.5
81	410.5
82	369.5
83	294.0
84	221.0
85	174.0
86	155.5
87	125.0
88	94.0
89	69.0
90	56.0
91	50.0
92	30.5
93	19.5
94	18.5
95	13.5
96	7.5
97	4.0
98	2.5
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.053439072947032325
2	0.09412830561894962
3	0.08467442221348252
4	0.05647698337136977
5	0.08655112363392264
6	0.001395796681452337
7	0.0010791033167530672
8	0.004844235541511052
9	0.009735388618533106
10-11	0.010116593594560006
12-13	0.01635076112558452
14-15	0.011846677716528238
16-17	0.0015482786718630963
18-19	6.157926535819134E-4
20-21	1.2902322265525803E-4
22-23	3.9293435990464943E-4
24-25	8.679744069535542E-4
26-27	0.001395796681452337
28-29	0.0015013611363520936
30-31	0.004797318006000049
32-33	2.345876775550146E-4
34-35	0.002381064927183398
36-37	4.3985189541565243E-4
38-39	0.0025159528417775315
40-41	0.0031552042631149465
42-43	0.001219855923286076
44-45	6.744395729706671E-4
46-47	0.0011260208522640702
48-49	0.002293094548100268
50-51	0.004175660660479261
52-53	0.13328685369482043
54-55	0.14313953615213104
56-57	0.1293399160199573
58-59	0.1228946195791333
60-61	0.11093651271576642
62-63	0.11551683712002808
64-65	0.11816181318446087
66-67	0.1263665172069475
68-69	0.13904011648685716
70-71	0.12612020014551473
72-73	0.11792136081496696
74-75	0.12312334256474942
76-77	0.13456535653749527
78-79	0.1484822705084465
80-81	0.1385533470559305
82-83	0.11821459541191073
84-85	0.12465402716079589
86-87	0.12562170133071032
88-89	0.12199145702054648
90-91	0.13252444374276662
92-93	0.14896317524743427
94-95	0.15815901220759085
96-97	0.15895661031127792
98-99	0.14190795084496724
100-101	0.13124594090009178
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8525597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.95031884457863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.87441562143674	42.21658691464354
2	4.270021591684459	3.9241770722227205
3	1.2980018877129844	1.7893080180382974
4	0.6416587058996558	1.1793768850195556
5	0.36920610566584355	0.8482569137355348
6	0.23510724079334194	0.648195160627391
7	0.17611569508591712	0.5664800639912737
8	0.12303026109649952	0.4522623779932728
9	0.10691408018508242	0.44214624661314444
>10	0.8535074667873892	7.973831993772245
>50	0.0296916007956007	0.8780111797251765
>100	0.014266478910576409	1.460005388453901
>500	0.0037413544708688306	1.156049075154298
>1k	0.0035478361361681392	3.222099244003697
>5k	5.805550041002409E-4	1.9766536248091122
>10k+	1.9351833470008029E-4	31.266559841196816
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1022639	11.99492539935913	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	20478	0.24019432304857946	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	11733	0.13762086103764934	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGACACACAGCAGCAGCAG	9225	0.1082035662722505	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA	8876	0.10411001129891548	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC	8863	0.10395752930850474	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC	8634	0.10127150040049981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1729383877750731E-5	0.0	0.0	0.0	0.0
2	5.864691938875365E-5	0.0	0.0	0.0	0.0
3	5.864691938875365E-5	0.0	0.0	1.1729383877750731E-5	0.0
4	8.210568714425511E-5	0.0	0.0	1.1729383877750731E-5	0.0
5	9.383507102200585E-5	0.0	0.0	1.1729383877750731E-5	0.0
6	1.2902322265525806E-4	0.0	0.0	1.1729383877750731E-5	0.0
7	1.876701420440117E-4	0.0	0.0	2.3458767755501462E-5	0.0
8	1.876701420440117E-4	0.0	0.0	2.3458767755501462E-5	0.0
9	2.345876775550146E-4	0.0	0.0	2.3458767755501462E-5	0.0
10-11	2.8150521306601756E-4	5.8646919388753655E-6	0.0	2.3458767755501462E-5	0.0
12-13	3.1082867276039435E-4	2.3458767755501462E-5	0.0	3.5188151633252195E-5	5.8646919388753655E-6
14-15	3.6947559214914804E-4	2.3458767755501462E-5	0.0	3.5188151633252195E-5	2.3458767755501462E-5
16-17	4.105284357212756E-4	2.3458767755501462E-5	0.0	3.5188151633252195E-5	3.5188151633252195E-5
18-19	4.5744597123227853E-4	2.3458767755501462E-5	0.0	3.5188151633252195E-5	3.5188151633252195E-5
20-21	4.867694309266553E-4	2.3458767755501462E-5	0.0	5.278222744987829E-5	3.5188151633252195E-5
22-23	5.102281986821568E-4	4.1052843572127556E-5	0.0	7.037630326650439E-5	3.5188151633252195E-5
24-25	5.806045019486612E-4	4.6917535511002924E-5	0.0	7.037630326650439E-5	3.5188151633252195E-5
26-27	6.216573455207887E-4	4.6917535511002924E-5	0.0	7.624099520537975E-5	3.5188151633252195E-5
28-29	6.392514213374148E-4	4.6917535511002924E-5	0.0	8.797037908313049E-5	3.5188151633252195E-5
30-31	6.803042649095424E-4	4.6917535511002924E-5	0.0	9.969976296088121E-5	3.5188151633252195E-5
32-33	7.624099520537975E-4	4.6917535511002924E-5	0.0	1.0556445489975658E-4	3.5188151633252195E-5
34-35	8.386509472591773E-4	5.864691938875365E-5	0.0	1.0556445489975658E-4	3.5188151633252195E-5
36-37	9.383507102200584E-4	6.451161132762901E-5	0.0	1.172938387775073E-4	3.5188151633252195E-5
38-39	9.911329376699367E-4	7.037630326650439E-5	0.0	1.2902322265525806E-4	3.5188151633252195E-5
40-41	0.0010380504731809397	7.037630326650439E-5	0.0	1.4661729847188413E-4	4.1052843572127556E-5
42-43	0.0010791033167530672	7.037630326650439E-5	0.0	1.524819904107595E-4	4.6917535511002924E-5
44-45	0.0011260208522640702	8.210568714425511E-5	0.0	1.7594075816626097E-4	4.6917535511002924E-5
46-47	0.0011846677716528238	8.210568714425511E-5	0.0	1.7594075816626097E-4	4.6917535511002924E-5
48-49	0.0012785028426748296	8.210568714425511E-5	0.0	1.8180545010513632E-4	4.6917535511002924E-5
50-51	0.0013782026056357109	8.210568714425511E-5	0.0	1.9353483398288707E-4	4.6917535511002924E-5
52-53	0.0014661729847188414	8.210568714425511E-5	0.0	2.052642178606378E-4	4.6917535511002924E-5
54-55	0.0016127902831907254	8.210568714425511E-5	0.0	2.228582936772639E-4	4.6917535511002924E-5
56-57	0.0017359488139071081	8.210568714425511E-5	0.0	2.345876775550146E-4	4.6917535511002924E-5
58-59	0.0018356485768679894	8.210568714425511E-5	0.0	2.345876775550146E-4	4.6917535511002924E-5
60-61	0.0019177542640122446	8.797037908313049E-5	0.0	2.4045236949389E-4	4.6917535511002924E-5
62-63	0.0020526421786063783	9.383507102200585E-5	0.0	2.580464453105161E-4	5.278222744987829E-5
64-65	0.002181665401261636	9.383507102200585E-5	0.0	2.7564052112714215E-4	5.864691938875365E-5
66-67	0.0024279824626944014	9.383507102200585E-5	0.0	2.8150521306601756E-4	5.864691938875365E-5
68-69	0.0026918935999437925	9.383507102200585E-5	0.0	2.873699050048929E-4	5.864691938875365E-5
70-71	0.002908887201682181	1.0556445489975658E-4	0.0	3.04963980821519E-4	4.8090473898778E-4
72-73	0.003178663030870448	1.0556445489975658E-4	0.0	3.1669336469926975E-4	4.8090473898778E-4
74-75	0.0033897919406699613	1.0556445489975658E-4	0.0	3.1669336469926975E-4	4.8090473898778E-4
76-77	0.0036595677698582282	1.172938387775073E-4	0.0	3.2842274857702045E-4	4.8090473898778E-4
78-79	0.004029043362007377	1.172938387775073E-4	0.0	3.2842274857702045E-4	4.926341228655307E-4
80-81	0.004515812792934032	1.172938387775073E-4	0.0	3.2842274857702045E-4	4.926341228655307E-4
82-83	0.0050846879110049426	1.172938387775073E-4	0.0	3.2842274857702045E-4	4.926341228655307E-4
84-85	0.0058529625549976145	1.172938387775073E-4	0.0	3.2842274857702045E-4	5.160928906210322E-4
86-87	0.006961389331445059	1.172938387775073E-4	0.0	3.2842274857702045E-4	5.160928906210322E-4
88-89	0.008404103548408398	1.2902322265525806E-4	0.0	3.3428744051589585E-4	5.160928906210322E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	239480	0.0	91.313545	1
AGTGGTA	238990	0.0	91.177826	5
TGGTATC	240060	0.0	90.79948	7
GGTATCA	240350	0.0	90.78669	8
GTATCAA	240515	0.0	90.74917	9
GTGGTAT	240480	0.0	90.67393	6
AGCAGTG	242300	0.0	90.200134	2
GCAGTGG	242000	0.0	90.170074	3
CAGTGGT	243040	0.0	89.604614	4
CAACGCA	239170	0.0	46.00176	12-13
ATCAACG	239455	0.0	45.999676	10-11
TCAACGC	239115	0.0	45.916058	12-13
ACGCAGA	240290	0.0	45.80356	14-15
AACGCAG	240420	0.0	45.639603	14-15
CGCAGAG	240655	0.0	45.55226	16-17
TATCAAC	241205	0.0	45.47012	10-11
AGAGTAC	235905	0.0	45.420506	18-19
CAGAGTA	239445	0.0	45.23064	18-19
GCAGAGT	242545	0.0	45.166977	16-17
GAGTACT	129395	0.0	45.03008	20-21
>>END_MODULE