FastQCFastQC Report
Sat 18 Jun 2016
SRR578593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8525597
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA683720.8019614344895729No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122030.14313367146019218No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93060.10915364636634829No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85430.10020412646762449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3750.025.2894173
CGTACGC2000.022.52160320-21
TCAACGC86400.020.32931910-11
CGCGTAG2350.019.16479354-55
ATCAACG89250.019.09585210-11
ACTCGCG2500.018.96305850-51
CAACGCA89800.018.952512-13
AACGCAG93250.018.88679912-13
GTATCAA107950.018.5356128
GGTATCA107800.018.4734367
CTCGCGT2600.018.23370752-53
AGTGGTA112200.017.7914564
ACGTACG2550.017.66400320-21
TATCAAC114500.017.6393689
TGGTATC113550.017.6214856
CGCAGAG104000.017.09410314-15
ACGCAGA102600.016.77287914-15
GTGGTAT120550.016.5591165
AGCAGTG141300.014.801661
AGAGTAC113200.014.65848618-19
Produced by FastQC (version 0.11.5)