##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578593_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8525597
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99423559429328	31.0	31.0	34.0	30.0	34.0
2	32.263105797752345	34.0	31.0	34.0	30.0	34.0
3	32.33394318309908	34.0	31.0	34.0	30.0	34.0
4	35.90733329290606	37.0	35.0	37.0	35.0	37.0
5	35.78928314345611	37.0	35.0	37.0	33.0	37.0
6	35.76171205371307	37.0	35.0	37.0	33.0	37.0
7	35.75716292946993	37.0	35.0	37.0	33.0	37.0
8	35.67500633679964	37.0	35.0	37.0	33.0	37.0
9	37.31345194946466	39.0	37.0	39.0	34.0	39.0
10-11	37.3121747368542	39.0	37.0	39.0	33.5	39.0
12-13	37.3056578325248	39.0	37.0	39.0	33.5	39.0
14-15	38.62128223982438	40.0	38.0	41.0	34.0	41.0
16-17	38.57886996066082	40.0	38.0	41.0	34.0	41.0
18-19	38.538195624306425	40.0	38.0	41.0	34.0	41.0
20-21	38.47736615981262	40.0	38.0	41.0	34.0	41.0
22-23	38.361778829095485	40.0	38.0	41.0	33.5	41.0
24-25	38.28355527478017	40.0	38.0	41.0	33.0	41.0
26-27	38.11832408921041	40.0	38.0	41.0	33.0	41.0
28-29	38.023491551383444	40.0	37.0	41.0	33.0	41.0
30-31	37.92285566629528	40.0	37.0	41.0	33.0	41.0
32-33	37.79262214716459	40.0	37.0	41.0	32.5	41.0
34-35	37.65914281428034	40.0	37.0	41.0	32.0	41.0
36-37	37.532728793068685	40.0	37.0	41.0	32.0	41.0
38-39	37.4018313321636	40.0	36.5	41.0	31.0	41.0
40-41	37.252670575444746	40.0	36.0	41.0	31.0	41.0
42-43	37.0943804287254	39.0	36.0	41.0	31.0	41.0
44-45	36.939926670237874	39.0	36.0	41.0	30.5	41.0
46-47	37.01888653662611	39.0	36.0	41.0	31.0	41.0
48-49	36.99640400549076	39.0	36.0	41.0	31.0	41.0
50-51	36.97051930791474	39.0	36.0	41.0	31.0	41.0
52-53	36.98124066854204	39.0	36.0	41.0	31.0	41.0
54-55	36.85303293130088	39.0	35.5	41.0	31.0	41.0
56-57	36.70569310278213	39.0	35.0	41.0	30.0	41.0
58-59	36.49742158818907	39.0	35.0	41.0	30.0	41.0
60-61	36.27557812080491	39.0	35.0	41.0	30.0	41.0
62-63	36.01151092410303	38.0	35.0	40.0	29.0	41.0
64-65	35.71010176765334	38.0	34.5	40.0	29.0	41.0
66-67	35.39682740106059	37.0	34.0	40.0	29.0	41.0
68-69	35.07239340541196	37.0	34.0	39.5	28.5	41.0
70-71	34.712459315165844	36.0	34.0	39.0	28.0	41.0
72-73	34.29654943812146	36.0	34.0	39.0	28.0	40.5
74-75	33.912300921565965	35.0	33.0	38.5	27.5	40.0
76-77	32.46725496173464	34.5	31.5	36.5	26.0	39.0
78-79	33.06473071621846	35.0	32.5	37.0	26.0	39.0
80-81	33.00332832996915	35.0	33.0	36.5	26.0	39.0
82-83	32.7209207167545	35.0	33.0	36.0	26.0	38.0
84-85	32.42575769180739	35.0	33.0	36.0	26.0	37.0
86-87	32.133884348509554	35.0	32.5	35.0	26.0	37.0
88-89	31.876217348767483	35.0	32.0	35.0	25.0	36.0
90-91	31.628293654978062	35.0	32.0	35.0	25.0	36.0
92-93	31.386197177746027	35.0	32.0	35.0	24.5	36.0
94-95	31.13270255443695	35.0	32.0	35.0	24.0	36.0
96-97	30.53304390296656	34.0	31.5	35.0	18.5	35.0
98-99	30.504746177892294	34.0	31.5	35.0	19.0	35.0
100-101	29.92345685586593	34.0	30.5	35.0	9.5	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.43378180802677946
1101	2	0.38356998723800473
1101	3	0.3939976451956113
1101	4	0.29168999036066623
1101	5	0.29497860316882196
1101	6	0.2652889587124818
1101	7	0.29659547793121277
1101	8	0.3707666594541337
1101	9	0.47068076609932064
1101	10-11	0.41815798339071364
1101	12-13	0.40937305677766744
1101	14-15	0.5759171488628496
1101	16-17	0.5664582473566071
1101	18-19	0.5632975558457574
1101	20-21	0.6173272760733397
1101	22-23	0.629296952837791
1101	24-25	0.6803212984721512
1101	26-27	0.720290875595154
1101	28-29	0.7255067994721358
1101	30-31	0.7375023790447344
1101	32-33	0.6613536920963412
1101	34-35	0.6878315996861346
1101	36-37	0.7061050339074768
1101	38-39	0.6873137032076002
1101	40-41	0.7267264086436995
1101	42-43	0.7161303040866116
1101	44-45	0.7088742058499875
1101	46-47	0.6941603290167748
1101	48-49	0.6844149685809455
1101	50-51	0.6603997789282161
1101	52-53	0.5718772830882983
1101	54-55	0.703090546514126
1101	56-57	0.6878042161330811
1101	58-59	0.7183645966106198
1101	60-61	0.6562003881430059
1101	62-63	0.6941898938731725
1101	64-65	0.6737836498255731
1101	66-67	0.6765370719812083
1101	68-69	0.6929693312060081
1101	70-71	0.709962916118414
1101	72-73	0.6668884448783032
1101	74-75	0.6902743035981658
1101	76-77	0.6743253868306454
1101	78-79	0.5791887524655976
1101	80-81	0.5125449376580917
1101	82-83	0.5088075785956221
1101	84-85	0.4554272564812507
1101	86-87	0.45203719918134055
1101	88-89	0.465339302326246
1101	90-91	0.5007384463266575
1101	92-93	0.553093302154803
1101	94-95	0.5409567571897504
1101	96-97	0.8969454813580313
1101	98-99	0.6318909566996176
1101	100-101	0.618757464710967
1102	1	0.39842324779098703
1102	2	0.3741353427912486
1102	3	0.35883467598139873
1102	4	0.268704461364095
1102	5	0.28355475892505666
1102	6	0.29378293302507785
1102	7	0.25834881349206285
1102	8	0.2783765430632812
1102	9	0.42850780610001493
1102	10-11	0.3779954483767938
1102	12-13	0.3886473460512647
1102	14-15	0.46853957981549854
1102	16-17	0.5138886683015969
1102	18-19	0.5182834474169695
1102	20-21	0.5715207802549784
1102	22-23	0.5650679149479387
1102	24-25	0.6244255577545914
1102	26-27	0.6180847417300015
1102	28-29	0.6712092425813196
1102	30-31	0.6933324298935801
1102	32-33	0.7331706812578318
1102	34-35	0.7510236408726456
1102	36-37	0.7653478293301461
1102	38-39	0.7553915823987793
1102	40-41	0.7627489926298381
1102	42-43	0.7685803642237445
1102	44-45	0.8114333678482595
1102	46-47	0.810259179036926
1102	48-49	0.8181349196312269
1102	50-51	0.8358731630402119
1102	52-53	0.7712374524098067
1102	54-55	0.7492667366849517
1102	56-57	0.6981535258403468
1102	58-59	0.8200065593973278
1102	60-61	0.8215031784195546
1102	62-63	0.7755581374449037
1102	64-65	0.7376866973222604
1102	66-67	0.7479754738541686
1102	68-69	0.8279506755940105
1102	70-71	0.7684927337848322
1102	72-73	0.7859205334309465
1102	74-75	0.6676820459490216
1102	76-77	0.6759522155544602
1102	78-79	0.7068746213919681
1102	80-81	0.6773992293113622
1102	82-83	0.6876283709872979
1102	84-85	0.6619708074124091
1102	86-87	0.6035138899655408
1102	88-89	0.5968454265859471
1102	90-91	0.6252799819899337
1102	92-93	0.6839490420709211
1102	94-95	0.6995122679413335
1102	96-97	1.1369146164412456
1102	98-99	0.928096287307639
1102	100-101	0.8912204024623982
1103	1	0.3342341602546455
1103	2	0.2999429624534997
1103	3	0.2732965503675828
1103	4	0.23641932924358855
1103	5	0.2213825013976063
1103	6	0.2894305145522935
1103	7	0.2796588553556987
1103	8	0.0899458654745473
1103	9	0.20244237909971474
1103	10-11	0.40305744341510774
1103	12-13	0.3374489373607048
1103	14-15	0.455253842350821
1103	16-17	0.4575537932635356
1103	18-19	0.40018604421582893
1103	20-21	0.4280651687892245
1103	22-23	0.45081186452301836
1103	24-25	0.26511403538911793
1103	26-27	0.2447745074896801
1103	28-29	0.5020566454135107
1103	30-31	0.3290102152558845
1103	32-33	0.501803479382886
1103	34-35	0.4658260636730347
1103	36-37	0.5610594438211436
1103	38-39	0.6278676865832509
1103	40-41	0.4804127409177852
1103	42-43	0.6005739970414083
1103	44-45	0.6725181935550637
1103	46-47	0.5427907373653937
1103	48-49	0.5691740532693856
1103	50-51	0.5390346170017253
1103	52-53	0.6270489864515767
1103	54-55	0.7308854389635329
1103	56-57	0.6120966168910371
1103	58-59	0.3536839483733587
1103	60-61	0.3365410716794841
1103	62-63	0.1962551695916872
1103	64-65	0.47169741190531056
1103	66-67	0.43583233760885065
1103	68-69	0.3928680165557452
1103	70-71	0.4361952482332683
1103	72-73	0.6002801512715266
1103	74-75	0.513791891477311
1103	76-77	0.3591733566416693
1103	78-79	0.4371965997258087
1103	80-81	0.4288846664565753
1103	82-83	0.49425939414221176
1103	84-85	0.5315057532548124
1103	86-87	0.38761217674518633
1103	88-89	0.3660870587424405
1103	90-91	0.4494194109905578
1103	92-93	0.4265644813248137
1103	94-95	0.4019580649837522
1103	96-97	0.5751178928003498
1103	98-99	0.3487378896179649
1103	100-101	0.635254524323134
1104	1	0.30616577906226894
1104	2	0.2852724639571562
1104	3	0.26923290546701395
1104	4	0.17845005682595882
1104	5	0.21857092358893482
1104	6	0.22699229848944213
1104	7	0.20337436692977917
1104	8	0.28208833684312395
1104	9	0.2994287465034162
1104	10-11	0.31172318252964715
1104	12-13	0.30556526565494124
1104	14-15	0.3309847412221245
1104	16-17	0.38264289602725654
1104	18-19	0.40464436160978323
1104	20-21	0.34121553610388133
1104	22-23	0.4427714898299584
1104	24-25	0.4114732788860067
1104	26-27	0.5036486372725051
1104	28-29	0.48974656321937005
1104	30-31	0.5059952163282091
1104	32-33	0.5457787208068083
1104	34-35	0.5742335848816751
1104	36-37	0.6029073790798449
1104	38-39	0.6043069369114562
1104	40-41	0.536971153993079
1104	42-43	0.6387198867692803
1104	44-45	0.5914319414002591
1104	46-47	0.4270488388505598
1104	48-49	0.5588476321651612
1104	50-51	0.4806777637699824
1104	52-53	0.5625948439782675
1104	54-55	0.5969655018903595
1104	56-57	0.6019315587926641
1104	58-59	0.5577803907737717
1104	60-61	0.5163650257252641
1104	62-63	0.398301598370395
1104	64-65	0.5111859241476182
1104	66-67	0.5485628119848158
1104	68-69	0.6212198638350444
1104	70-71	0.570137063596377
1104	72-73	0.7016897798408834
1104	74-75	0.662099985990416
1104	76-77	0.5180121511054239
1104	78-79	0.5363808555252234
1104	80-81	0.6909059573037624
1104	82-83	0.7180704587705549
1104	84-85	0.7179702580045841
1104	86-87	0.6840803124486214
1104	88-89	0.6321746813889781
1104	90-91	0.6383055274621796
1104	92-93	0.6858499753095941
1104	94-95	0.7247050899627574
1104	96-97	1.0869350174775114
1104	98-99	0.8572986177945765
1104	100-101	0.8771034251319172
1105	1	0.1320559089948219
1105	2	0.14001771877830294
1105	3	0.1294026564223003
1105	4	0.060155392947670805
1105	5	0.12378218750423287
1105	6	0.06278076698934854
1105	7	0.054120169927934114
1105	8	0.17507000393933225
1105	9	0.113863886829769
1105	10-11	0.11694103077950757
1105	12-13	0.1393122206084385
1105	14-15	0.16505261001862692
1105	16-17	0.15676196457201286
1105	18-19	0.07357055919568722
1105	20-21	0.16611751941127295
1105	22-23	0.15379792328443642
1105	24-25	0.14402899371124533
1105	26-27	0.15667950089597582
1105	28-29	0.1818017236171201
1105	30-31	0.14292974086836097
1105	32-33	0.20777301545859217
1105	34-35	0.14636783872377634
1105	36-37	0.1583076609088252
1105	38-39	0.20063806386299632
1105	40-41	0.24702806234140695
1105	42-43	0.1420528254823381
1105	44-45	0.27986920953086525
1105	46-47	0.2476448332775263
1105	48-49	0.1867268175356429
1105	50-51	0.14401993454504947
1105	52-53	0.27689291073341593
1105	54-55	0.3572172661601698
1105	56-57	0.33209861151016895
1105	58-59	0.3019663653801672
1105	60-61	0.26221499248306657
1105	62-63	0.33579286734685354
1105	64-65	0.2823569953282927
1105	66-67	0.2602639481526907
1105	68-69	0.23654158837548778
1105	70-71	0.240766588752102
1105	72-73	0.26831050809640544
1105	74-75	0.34283765416015655
1105	76-77	0.3075009729830569
1105	78-79	0.3139266933821645
1105	80-81	0.29499558072566856
1105	82-83	0.25306624695544144
1105	84-85	0.308965585092821
1105	86-87	0.2443818687078405
1105	88-89	0.22190037338647883
1105	90-91	0.3049435605030766
1105	92-93	0.29065398670397613
1105	94-95	0.3592436357274771
1105	96-97	0.7398435601441733
1105	98-99	0.5107388982865615
1105	100-101	0.4124467450369629
1106	1	-0.056614396823103874
1106	2	-0.08538531002481164
1106	3	-0.07743251043258681
1106	4	-0.06395899824008922
1106	5	-0.03789127135441106
1106	6	-0.06975851031961611
1106	7	-0.07597959486854933
1106	8	-0.058978753835098985
1106	9	-0.03012405069519275
1106	10-11	-0.0897121386955746
1106	12-13	-0.08149507042408999
1106	14-15	-0.21461687138886276
1106	16-17	-0.14642980822356577
1106	18-19	-0.1462849829496804
1106	20-21	-0.17568144842520184
1106	22-23	-0.15507712099084614
1106	24-25	-0.06054705422937445
1106	26-27	-0.0745663711096185
1106	28-29	-0.13401377198620423
1106	30-31	-0.2158678568232304
1106	32-33	-0.2959623647229037
1106	34-35	-0.37511369422513496
1106	36-37	-0.21232251018029302
1106	38-39	-0.32676616181596074
1106	40-41	-0.2817176038708098
1106	42-43	-0.21242505768406517
1106	44-45	-0.22307339926847192
1106	46-47	-0.267385180419069
1106	48-49	-0.2891621298102578
1106	50-51	-0.25751649066418025
1106	52-53	-0.14876909211611888
1106	54-55	-0.19779596900968244
1106	56-57	-0.15851461236712083
1106	58-59	-0.1272771031237241
1106	60-61	-0.24975737064286818
1106	62-63	-0.19457467380139803
1106	64-65	-0.15407866970906525
1106	66-67	-0.1847977457181571
1106	68-69	-0.1292421214283479
1106	70-71	-0.14306188946638798
1106	72-73	-0.14701310920251842
1106	74-75	-0.13932010563500086
1106	76-77	-0.20915338347231227
1106	78-79	-0.12728503026262672
1106	80-81	-0.19878773730675903
1106	82-83	-0.17008502213846555
1106	84-85	-0.17502058275463384
1106	86-87	-0.1972735385428166
1106	88-89	-0.23788916232232182
1106	90-91	-0.15594482633782647
1106	92-93	-0.13591082998736326
1106	94-95	-0.08005295135734514
1106	96-97	0.1696175317133033
1106	98-99	-0.04357992673100597
1106	100-101	-0.007372214516355058
1107	1	-0.17558492474227805
1107	2	-0.22290510988810297
1107	3	-0.19067057821583688
1107	4	-0.15286707709487501
1107	5	-0.15625721429972117
1107	6	-0.14328913854944147
1107	7	-0.16267792433514217
1107	8	-0.14846566574926356
1107	9	-0.23915209417177152
1107	10-11	-0.22048167179141842
1107	12-13	-0.20720029919804972
1107	14-15	-0.27168443085350447
1107	16-17	-0.30086313521819363
1107	18-19	-0.2965540924153345
1107	20-21	-0.2965628887606826
1107	22-23	-0.19903481186056382
1107	24-25	-0.18724406900474833
1107	26-27	-0.18562722788835373
1107	28-29	-0.1990962005614776
1107	30-31	-0.29975661603194226
1107	32-33	-0.3793979266254226
1107	34-35	-0.3383738954989681
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2308	16-17	-0.9230409654239082
2308	18-19	-0.9429285510786585
2308	20-21	-0.9678061522898105
2308	22-23	-1.0412156335571368
2308	24-25	-1.185011778200888
2308	26-27	-1.1812095464480308
2308	28-29	-1.1908111034985964
2308	30-31	-1.084845753607361
2308	32-33	-1.0996340293896836
2308	34-35	-1.21839300094436
2308	36-37	-1.2845615947495475
2308	38-39	-1.2768947346224948
2308	40-41	-1.2723399747673483
2308	42-43	-1.1793780055094985
2308	44-45	-1.1760221202334336
2308	46-47	-1.155392581142614
2308	48-49	-1.2173114118329451
2308	50-51	-1.2750829387150944
2308	52-53	-1.1974266328769474
2308	54-55	-1.1761816522521613
2308	56-57	-1.1080642357718133
2308	58-59	-1.1395045699174347
2308	60-61	-1.1103339322260837
2308	62-63	-1.187910938260913
2308	64-65	-1.1646153728239028
2308	66-67	-1.0703606028541657
2308	68-69	-1.1724346469695632
2308	70-71	-1.0771829371129726
2308	72-73	-1.172452982661575
2308	74-75	-1.2277889341436747
2308	76-77	-1.194647508190961
2308	78-79	-1.5794169041936712
2308	80-81	-1.2560654166006238
2308	82-83	-1.1643725762618686
2308	84-85	-1.048207605950843
2308	86-87	-1.0316390545319933
2308	88-89	-1.0839583414768121
2308	90-91	-1.1423301836522661
2308	92-93	-1.0547196997001755
2308	94-95	-1.1773134874220084
2308	96-97	-1.0078004137601368
2308	98-99	-1.288844462648946
2308	100-101	-1.271502115149378
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32.0
3	72.0
4	139.0
5	386.0
6	834.0
7	1631.0
8	3440.0
9	5515.0
10	7331.0
11	9037.0
12	10792.0
13	12583.0
14	13888.0
15	15584.0
16	18015.0
17	21218.0
18	24638.0
19	28918.0
20	30589.0
21	30797.0
22	33355.0
23	38739.0
24	47193.0
25	59548.0
26	76044.0
27	97206.0
28	123151.0
29	154729.0
30	193158.0
31	237368.0
32	293544.0
33	367340.0
34	482394.0
35	694419.0
36	988157.0
37	1673437.0
38	2073669.0
39	653913.0
40	2794.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	18.4772567333093	31.574904543045985	27.54474355925839	22.403095164386325
2	22.891225343517256	28.95321999252466	28.4016144483839	19.75394021557419
3	20.895071919766572	28.084338939311742	28.889453233823552	22.131135907098137
4	21.452100696822914	28.817453696839806	28.48213230064165	21.248313305695632
5	22.374224680723287	28.479717366213443	28.338869652525595	20.80718830053768
6	22.05904173045008	28.262114664814675	28.309689045822832	21.369154558912413
7	22.281771000904687	28.59338765367399	28.107439279618777	21.01740206580255
8	21.387728988925527	28.347148319614927	28.58304493378883	21.68207775767072
9	21.15221470476312	28.224752215549366	29.033949156033707	21.589083923653813
10-11	21.279219508029758	28.72985903509162	28.364365568768967	21.626555888109653
12-13	21.356785923613327	28.785233456378478	28.07557054362293	21.78241007638527
14-15	21.546403143381042	28.714129931311554	27.924601643732398	21.814865281575003
16-17	21.76716774203613	28.538892936177955	27.847709667721805	21.84622965406411
18-19	21.773806573310935	28.5698409155394	27.839663310381667	21.816689200767993
20-21	21.82323419697178	28.45695732509993	27.957825123566128	21.76198335436216
22-23	21.69769736730213	28.47382157408714	27.9830578274416	21.845423231169132
24-25	21.732224057564853	28.354465970425103	27.954059035785896	21.95925093622415
26-27	21.97134074769586	28.1889665966591	28.116362750842942	21.7233299048021
28-29	21.983457580741852	28.259974052256986	28.00286595765669	21.753702409344474
30-31	21.882232997876862	28.28533884489262	27.980814715966517	21.851613441263996
32-33	21.929349932913787	28.2831630441833	27.917165214353908	21.870321808549008
34-35	21.927848571777435	28.35610221782709	27.903564993747654	21.81248421664782
36-37	21.978765827190752	28.327054398653846	27.826555723898277	21.867624050257124
38-39	21.967787123881177	28.3316347230581	27.84931072862112	21.8512674244396
40-41	21.967787123881177	28.361667810477087	27.833446736926458	21.83709832871528
42-43	21.954702996165548	28.31214635174522	27.835135768204854	21.89801488388438
44-45	21.936528315846974	28.30417623540029	27.850313590942665	21.908981857810076
46-47	21.98577084816123	28.33668754653634	27.83448805221359	21.84305355308884
48-49	21.965716887626755	28.354008522804914	27.75813822773936	21.922136361828972
50-51	21.992213565806594	28.35059527209649	27.773621014457987	21.883570147638927
52-53	21.98220840135887	28.377918871839707	27.689826296035342	21.95004643076608
54-55	22.031518731180938	28.411066110678227	27.593686401081357	21.963728757059474
56-57	22.015942109391283	28.45359099192702	27.59976222193003	21.93070467675167
58-59	22.04536526884862	28.495224440001092	27.535684597805876	21.923725693344405
60-61	21.989828509309735	28.474784278960502	27.620925885046837	21.914461326682925
62-63	21.98510073352716	28.479386317951693	27.58652116349753	21.948991785023615
64-65	22.002361483365313	28.55524616613768	27.573224252890437	21.869168097606572
66-67	21.985063423533045	28.561682273519995	27.518164010278944	21.935090292668015
68-69	21.962063785969132	28.608941755710525	27.54610422711714	21.8828902312032
70-71	21.99703082028252	28.621407534753914	27.501789612844252	21.879772032119313
72-73	21.94748742184637	28.60254747014446	27.49457794473125	21.95538716327792
74-75	21.996743569053244	28.64293931981142	27.494156857034497	21.866160254100844
76-77	21.97510557061214	28.67616912766149	27.483744116354863	21.86498118537151
78-79	22.000733271710967	28.682718682326698	27.423282268767085	21.89326577719525
80-81	21.98368218408827	28.71961522426103	27.44307593040927	21.85362666124143
82-83	21.96322394094689	28.786566934225633	27.3977247195311	21.85248440529638
84-85	21.965051981311117	28.777137243905145	27.399307416355427	21.85850335842831
86-87	21.94600280999026	28.83704358209404	27.370077878349313	21.84687572956639
88-89	21.954226651771	28.899364326589115	27.338079345567817	21.80832967607207
90-91	21.941783327219966	28.897564746129856	27.30694219006921	21.853709736580964
92-93	21.94296205889159	28.925099818746308	27.28538822708515	21.846549895276947
94-95	21.965822594693147	28.993999701494044	27.257404315747902	21.782773388064903
96-97	21.90255674081257	28.99244263116768	27.267210649695333	21.837789978324416
98-99	21.91709747854001	29.026276932027063	27.232714007395003	21.823911582037926
100-101	21.94561494526751	29.085493249698928	27.15885802418418	21.81003378084938
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	76103.0
1	43134.5
2	7637.5
3	4571.5
4	3726.5
5	3475.5
6	3490.5
7	3293.0
8	3043.5
9	2858.0
10	2886.5
11	3547.5
12	6831.0
13	6805.0
14	4222.5
15	4579.0
16	5289.0
17	6441.0
18	8123.0
19	10223.0
20	13246.0
21	17526.0
22	22882.0
23	29624.0
24	36972.0
25	46577.5
26	60500.5
27	76240.0
28	88936.5
29	100194.5
30	116743.5
31	137765.0
32	159349.0
33	181334.0
34	202447.5
35	220239.0
36	236046.0
37	249836.0
38	259655.0
39	265022.0
40	270846.0
41	277812.5
42	282922.0
43	289721.5
44	297368.0
45	304584.5
46	308122.0
47	308345.5
48	306795.0
49	302115.0
50	296399.0
51	288445.5
52	285341.0
53	281677.0
54	267510.0
55	245760.5
56	219764.0
57	194262.5
58	172844.0
59	150033.5
60	124159.5
61	101130.0
62	78841.5
63	57833.0
64	41789.0
65	28717.5
66	18348.0
67	12435.5
68	8232.5
69	5127.0
70	2780.0
71	1174.5
72	466.5
73	221.5
74	114.0
75	59.5
76	40.5
77	29.0
78	17.0
79	10.0
80	6.5
81	7.0
82	5.5
83	4.5
84	4.0
85	4.5
86	2.5
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012597358284704286
2	0.0184268620719464
3	0.005536269190298345
4	5.278222744987829E-4
5	9.031625585868063E-4
6	0.0
7	0.0
8	0.007929063501359494
9	0.00995824691221037
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.383507102200585E-5
24-25	0.004633106631711539
26-27	0.006644695966745789
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.1729383877750731E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	3.5188151633252195E-5
62-63	1.1142914683863194E-4
64-65	2.756405211271422E-4
66-67	1.8180545010513632E-4
68-69	2.697758291882668E-4
70-71	1.172938387775073E-4
72-73	1.7594075816626097E-5
74-75	6.451161132762901E-5
76-77	0.12526981981437782
78-79	0.05725112270730132
80-81	8.797037908313049E-5
82-83	0.0021875300932005116
84-85	0.009975840988026997
86-87	0.12040212550511126
88-89	0.13738040866815546
90-91	0.150482130459603
92-93	0.14857610557946851
94-95	0.1155813487313557
96-97	1.199857323774511
98-99	0.07746085112866583
100-101	0.07010652743731612
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8525597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.47831378530016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.4114930616457	60.76881919334871
2	4.476495486093059	5.951797431659483
3	1.2620064016806798	2.516881727099574
4	0.7013927101465021	1.86509618687365
5	0.4118520375375034	1.3689614492266684
6	0.27447886450208153	1.0948135249081357
7	0.17583328756248892	0.8182370325136039
8	0.12753923282843949	0.6782874511924364
9	0.08886437435322708	0.531679838632939
>10	0.960128545486105	14.109116310720033
>50	0.09195685932489056	3.981466021833223
>100	0.01743562432878454	1.5447886073029737
>500	1.30878627616715E-4	0.06351431046380204
>1k	6.54393138083575E-5	0.15778520825493836
>5k	1.963179414250725E-4	1.0435053185583696
>10k+	1.30878627616715E-4	3.5052503874115413
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68372	0.8019614344895729	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12203	0.14313367146019218	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9306	0.10915364636634829	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8543	0.10020412646762449	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3458767755501462E-5	0.0	0.0	0.0	2.3458767755501462E-5
2	3.5188151633252195E-5	0.0	0.0	1.1729383877750731E-5	2.3458767755501462E-5
3	5.864691938875365E-5	0.0	0.0	1.1729383877750731E-5	3.5188151633252195E-5
4	9.383507102200585E-5	0.0	0.0	1.1729383877750731E-5	4.6917535511002924E-5
5	1.2902322265525806E-4	0.0	0.0	1.1729383877750731E-5	5.864691938875365E-5
6	1.524819904107595E-4	0.0	0.0	1.1729383877750731E-5	7.037630326650439E-5
7	1.7594075816626097E-4	0.0	0.0	1.1729383877750731E-5	7.037630326650439E-5
8	1.7594075816626097E-4	0.0	0.0	1.1729383877750731E-5	8.210568714425511E-5
9	2.345876775550146E-4	0.0	0.0	2.3458767755501462E-5	8.210568714425511E-5
10-11	2.6391113724939146E-4	0.0	0.0	4.6917535511002924E-5	8.797037908313049E-5
12-13	3.2842274857702045E-4	5.8646919388753655E-6	0.0	4.6917535511002924E-5	9.383507102200585E-5
14-15	3.6361090021027264E-4	1.1729383877750731E-5	0.0	4.6917535511002924E-5	1.0556445489975658E-4
16-17	3.8706966796577414E-4	1.1729383877750731E-5	0.0	4.6917535511002924E-5	1.1142914683863194E-4
18-19	4.105284357212756E-4	1.1729383877750731E-5	0.0	4.6917535511002924E-5	1.172938387775073E-4
20-21	4.8090473898778003E-4	1.1729383877750731E-5	0.0	4.6917535511002924E-5	1.2902322265525806E-4
22-23	5.336869664376582E-4	1.1729383877750731E-5	0.0	5.278222744987829E-5	1.4075260653300878E-4
24-25	5.864691938875365E-4	2.9323459694376827E-5	0.0	7.037630326650439E-5	1.524819904107595E-4
26-27	6.509808052151656E-4	4.6917535511002924E-5	0.0	7.624099520537975E-5	1.524819904107595E-4
28-29	6.861689568484178E-4	4.6917535511002924E-5	0.0	9.383507102200585E-5	1.524819904107595E-4
30-31	7.448158762371714E-4	4.6917535511002924E-5	0.0	9.969976296088121E-5	1.700760662273856E-4
32-33	7.741393359315483E-4	4.6917535511002924E-5	0.0	1.1142914683863194E-4	1.9939952592176242E-4
34-35	8.679744069535541E-4	4.6917535511002924E-5	0.0	1.4075260653300878E-4	1.9939952592176242E-4
36-37	0.0010321857812420642	4.6917535511002924E-5	0.0	1.524819904107595E-4	2.052642178606378E-4
38-39	0.0011670736958361977	4.6917535511002924E-5	0.0	1.524819904107595E-4	2.345876775550146E-4
40-41	0.001249179382980453	5.864691938875365E-5	0.0	1.524819904107595E-4	2.4631706143276536E-4
42-43	0.0013606085298190848	7.037630326650439E-5	0.0	1.5834668234963488E-4	2.4631706143276536E-4
44-45	0.0014485789089022153	9.383507102200585E-5	0.0	1.6421137428851022E-4	2.873699050048929E-4
46-47	0.0015600080557408471	9.383507102200585E-5	0.0	1.700760662273856E-4	2.9909928888264365E-4
48-49	0.0016714372025794792	1.0556445489975658E-4	0.0	1.876701420440117E-4	3.1082867276039435E-4
50-51	0.0018004604252347372	1.0556445489975658E-4	0.0	1.9939952592176242E-4	3.2842274857702045E-4
52-53	0.0019060248801344938	1.0556445489975658E-4	0.0	2.1112890979951317E-4	3.3428744051589585E-4
54-55	0.0020585068705452534	1.0556445489975658E-4	0.0	2.4045236949389E-4	3.401521324547712E-4
56-57	0.0022403123206503894	1.1142914683863194E-4	0.0	2.4631706143276536E-4	3.401521324547712E-4
58-59	0.0023927943110611492	1.2315853071638268E-4	0.0	2.4631706143276536E-4	3.5188151633252195E-4
60-61	0.002539411609533033	1.2902322265525806E-4	0.0	2.4631706143276536E-4	3.577462082713973E-4
62-63	0.0026860289080049174	1.2902322265525806E-4	0.0	2.4631706143276536E-4	3.6947559214914804E-4
64-65	0.0028326462064768017	1.4075260653300878E-4	0.0	2.5218175337164076E-4	3.8706966796577414E-4
66-67	0.0030379104243374393	1.4075260653300878E-4	0.0	2.580464453105161E-4	4.0466374378240024E-4
68-69	0.0032138511825037003	1.4075260653300878E-4	0.0	2.580464453105161E-4	4.105284357212756E-4
70-71	0.003436709476180964	1.4075260653300878E-4	0.0	2.580464453105161E-4	4.105284357212756E-4
72-73	0.0037182146892469816	1.4075260653300878E-4	0.0	2.697758291882668E-4	4.281225115379017E-4
74-75	0.003946937674863121	1.4075260653300878E-4	0.0	2.7564052112714215E-4	4.3398720347677703E-4
76-77	0.004210848812112512	1.4075260653300878E-4	0.0	3.1669336469926975E-4	4.457165873545278E-4
78-79	0.004580324404261661	1.4075260653300878E-4	0.0	3.2842274857702045E-4	4.5744597123227853E-4
80-81	0.005067093835188316	1.4075260653300878E-4	0.0	3.3428744051589585E-4	4.691753551100292E-4
82-83	0.0056770217968313535	1.4075260653300878E-4	0.0	3.6361090021027264E-4	4.750400470489046E-4
84-85	0.006421837673068525	1.4075260653300878E-4	0.0	3.6947559214914804E-4	4.926341228655307E-4
86-87	0.007495076297882717	1.4075260653300878E-4	0.0	3.753402840880234E-4	4.926341228655307E-4
88-89	0.00888500828739618	1.4075260653300878E-4	0.0	3.753402840880234E-4	4.98498814804406E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCACG	375	0.0	25.289417	3
CGTACGC	200	0.0	22.521603	20-21
TCAACGC	8640	0.0	20.329319	10-11
CGCGTAG	235	0.0	19.164793	54-55
ATCAACG	8925	0.0	19.095852	10-11
ACTCGCG	250	0.0	18.963058	50-51
CAACGCA	8980	0.0	18.9525	12-13
AACGCAG	9325	0.0	18.886799	12-13
GTATCAA	10795	0.0	18.535612	8
GGTATCA	10780	0.0	18.473436	7
CTCGCGT	260	0.0	18.233707	52-53
AGTGGTA	11220	0.0	17.791456	4
ACGTACG	255	0.0	17.664003	20-21
TATCAAC	11450	0.0	17.639368	9
TGGTATC	11355	0.0	17.621485	6
CGCAGAG	10400	0.0	17.094103	14-15
ACGCAGA	10260	0.0	16.772879	14-15
GTGGTAT	12055	0.0	16.559116	5
AGCAGTG	14130	0.0	14.80166	1
AGAGTAC	11320	0.0	14.658486	18-19
>>END_MODULE