FastQCFastQC Report
Sat 18 Jun 2016
SRR578592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8748757
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97613411.157402131525656No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT225570.2578309124370468No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG93970.10740954400722297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2129700.091.708965
GGTATCA2140350.091.391738
GTATCAA2139650.091.3819
TGGTATC2139200.091.340387
AAGCAGT2148200.091.329441
GTGGTAT2152500.090.825876
AGCAGTG2171200.090.262432
CAGTGGT2163950.090.107994
GCAGTGG2173100.090.0804753
ATCAACG2130850.046.3350510-11
CAACGCA2133500.046.29693212-13
TCAACGC2129350.046.2343212-13
ACGCAGA2145700.046.10067714-15
AACGCAG2141750.046.0459514-15
AGAGTAC2101600.045.7960318-19
TATCAAC2144150.045.79501710-11
CGCAGAG2156550.045.7936916-17
CAGAGTA2146500.045.5229418-19
GCAGAGT2174850.045.36577616-17
GAGTACT1254000.045.26940520-21
Produced by FastQC (version 0.11.5)