##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578592_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8748757 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.5827331814108 31.0 31.0 34.0 27.0 34.0 2 30.879253247061268 31.0 31.0 34.0 27.0 34.0 3 30.95144304499485 31.0 31.0 34.0 27.0 34.0 4 34.35118131638586 37.0 35.0 37.0 30.0 37.0 5 34.45801900772876 37.0 35.0 37.0 30.0 37.0 6 34.423837009074546 37.0 35.0 37.0 30.0 37.0 7 34.42230341978866 37.0 35.0 37.0 30.0 37.0 8 34.37426939621251 37.0 35.0 37.0 30.0 37.0 9 35.763067484901 39.0 35.0 39.0 30.0 39.0 10-11 35.74320352022579 39.0 35.0 39.0 30.0 39.0 12-13 35.76856700900482 39.0 35.0 39.0 30.0 39.0 14-15 36.81396374364952 40.0 36.0 41.0 30.0 41.0 16-17 36.7727110262635 40.0 36.0 41.0 30.0 41.0 18-19 36.782262383101965 40.0 36.0 41.0 30.0 41.0 20-21 36.67543709352083 39.5 36.0 41.0 30.0 41.0 22-23 36.37668665388695 39.0 36.0 40.5 29.0 41.0 24-25 36.27926298558755 39.0 36.0 40.5 29.0 41.0 26-27 36.15080073660749 39.0 36.0 40.5 28.5 41.0 28-29 35.858527159915404 39.0 35.0 41.0 27.0 41.0 30-31 35.627617957613865 39.0 34.5 40.5 26.5 41.0 32-33 35.24937268231361 38.0 34.0 40.0 25.0 41.0 34-35 35.01774686392592 38.0 34.0 40.0 25.0 41.0 36-37 34.698212957566426 38.0 33.5 40.0 24.0 41.0 38-39 34.07911912515115 37.0 33.0 40.0 24.0 41.0 40-41 34.26398350074187 37.0 33.0 40.0 24.0 41.0 42-43 34.20022781521992 37.0 33.0 40.0 24.0 41.0 44-45 34.053912115743984 37.0 33.0 40.0 23.0 41.0 46-47 33.84099204035499 37.0 33.0 40.0 23.0 41.0 48-49 33.501182739445156 37.0 33.0 40.0 20.5 41.0 50-51 32.309359318129424 36.0 31.5 39.0 15.5 40.5 52-53 31.959901503722186 36.5 31.0 39.0 8.0 40.0 54-55 31.629059933885465 37.0 31.0 40.0 2.0 41.0 56-57 31.17461680556449 37.0 30.0 40.0 2.0 41.0 58-59 30.83696678282412 36.0 29.0 40.0 2.0 41.0 60-61 30.5241518309401 36.0 29.0 39.5 2.0 41.0 62-63 30.19460650238657 35.5 28.0 39.0 2.0 41.0 64-65 29.859170108393684 35.0 28.0 39.0 2.0 41.0 66-67 29.541077492494075 35.0 28.0 39.0 2.0 40.5 68-69 29.21511370129494 35.0 27.5 38.0 2.0 40.0 70-71 28.839832504206026 35.0 26.0 37.5 2.0 40.0 72-73 28.443550038022543 34.5 26.0 37.0 2.0 39.5 74-75 28.07673404347612 34.0 26.0 36.5 2.0 39.0 76-77 27.680671208492818 34.0 26.0 36.0 2.0 39.0 78-79 27.315160199328886 34.0 25.0 35.5 2.0 38.0 80-81 26.949987752545876 34.0 24.5 35.0 2.0 37.0 82-83 26.57492018580468 34.0 23.5 35.0 2.0 37.0 84-85 26.23023939286461 33.0 22.5 35.0 2.0 36.0 86-87 25.883875846591692 33.0 20.0 35.0 2.0 36.0 88-89 25.521116085405048 33.0 19.5 35.0 2.0 35.5 90-91 25.154095661818015 33.0 17.5 35.0 2.0 35.0 92-93 24.800134750570855 32.0 12.5 35.0 2.0 35.0 94-95 24.364109381481278 32.0 4.5 35.0 2.0 35.0 96-97 23.85943386014722 32.0 2.0 35.0 2.0 35.0 98-99 23.317616491119825 32.0 2.0 35.0 2.0 35.0 100-101 21.96191870456569 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5526931160769806 1101 2 0.667614846177571 1101 3 0.6249382251190703 1101 4 0.7405177134695222 1101 5 0.6110624194926118 1101 6 0.5475870120507125 1101 7 0.4666757694944579 1101 8 0.4882455138597308 1101 9 0.7053445859952063 1101 10-11 0.7338538491647384 1101 12-13 0.7456816102919959 1101 14-15 0.7819878242610869 1101 16-17 0.7977621053190589 1101 18-19 0.7841644705512607 1101 20-21 0.8197557049501754 1101 22-23 0.8200524150127109 1101 24-25 0.8030622577695041 1101 26-27 1.0418739827233168 1101 28-29 1.0145184888741952 1101 30-31 1.0269519166870893 1101 32-33 1.0181668740259937 1101 34-35 1.150471573739864 1101 36-37 1.118379674111246 1101 38-39 1.0329429121892915 1101 40-41 1.1099281690667269 1101 42-43 1.3088633801776055 1101 44-45 1.2819821380644143 1101 46-47 1.3037861661338823 1101 48-49 1.2301511403849261 1101 50-51 1.1683981661393474 1101 52-53 1.1355687043322682 1101 54-55 0.9677106984698121 1101 56-57 0.9183523832272869 1101 58-59 0.9293535882894304 1101 60-61 1.0219099306145125 1101 62-63 0.9941447526850773 1101 64-65 0.8023827538211705 1101 66-67 0.9111533345377119 1101 68-69 0.966350579206452 1101 70-71 0.9968437510713102 1101 72-73 1.061282847197969 1101 74-75 1.081507279933291 1101 76-77 1.1128521035731573 1101 78-79 1.0496553280191563 1101 80-81 1.086046558413063 1101 82-83 1.0684652521565319 1101 84-85 1.0174350191453954 1101 86-87 0.9662699886566308 1101 88-89 1.0924225638956209 1101 90-91 1.0536699531883578 1101 92-93 1.2256615378723659 1101 94-95 1.3211537255009524 1101 96-97 1.4709889174573796 1101 98-99 1.570544376817839 1101 100-101 1.9208939196903714 1102 1 0.5196030620986143 1102 2 0.5485441436478737 1102 3 0.5289972904547469 1102 4 0.5972384659352912 1102 5 0.5906158706333429 1102 6 0.48972251253114507 1102 7 0.4357751361391635 1102 8 0.56491578338143 1102 9 0.6736078899602091 1102 10-11 0.7404215911914491 1102 12-13 0.6490648024118499 1102 14-15 0.6116449267962736 1102 16-17 0.6210253791332008 1102 18-19 0.6250714584611785 1102 20-21 0.7918121176335831 1102 22-23 0.7490664411948842 1102 24-25 0.7217049063961198 1102 26-27 0.9291288535954862 1102 28-29 0.7815933913153472 1102 30-31 1.0080628939098872 1102 32-33 0.9972230698311151 1102 34-35 0.9327597966666232 1102 36-37 0.833450105491103 1102 38-39 0.9618794298559337 1102 40-41 1.0904952610823813 1102 42-43 1.227320237097615 1102 44-45 1.0953679886587722 1102 46-47 1.2168403260278708 1102 48-49 1.0106504293443592 1102 50-51 1.0808072794772912 1102 52-53 1.0651551290822603 1102 54-55 1.0377509748263982 1102 56-57 1.1979914888154042 1102 58-59 1.056315239924679 1102 60-61 1.2372366443428682 1102 62-63 1.2472385692834003 1102 64-65 1.0996919815036605 1102 66-67 1.2276643742247728 1102 68-69 1.2883771217402753 1102 70-71 1.21687989987851 1102 72-73 1.3390202649822776 1102 74-75 1.3910552893628179 1102 76-77 1.2733322893607024 1102 78-79 1.2499193976081813 1102 80-81 1.1652112921320672 1102 82-83 1.1690638975717569 1102 84-85 1.1422731142012985 1102 86-87 1.229628719068188 1102 88-89 1.2669029917218317 1102 90-91 1.4019196123276352 1102 92-93 1.491035489687956 1102 94-95 1.4327604775479337 1102 96-97 1.5363964874869218 1102 98-99 1.6506829054847643 1102 100-101 1.9673949482507282 1103 1 0.31250538137388517 1103 2 0.44118629227186545 1103 3 0.42065295041118134 1103 4 0.506929064622355 1103 5 0.4524784083274085 1103 6 0.35701661136990026 1103 7 0.25325252556103095 1103 8 0.39474913829754854 1103 9 0.405873441171515 1103 10-11 0.49975863610112015 1103 12-13 0.48637673851702345 1103 14-15 0.6131062112313259 1103 16-17 0.49238332254818573 1103 18-19 0.5254952582124943 1103 20-21 0.5734405792050907 1103 22-23 0.39751260050661585 1103 24-25 0.4887888330012018 1103 26-27 0.6448803770928677 1103 28-29 0.590380461275835 1103 30-31 0.6788741940746306 1103 32-33 0.6476999092105089 1103 34-35 0.6353067715833518 1103 36-37 0.7013553003474584 1103 38-39 0.7278745725318956 1103 40-41 0.703601000263177 1103 42-43 0.8446987050012638 1103 44-45 0.7317369940787231 1103 46-47 0.6231288460099833 1103 48-49 0.8770587039646145 1103 50-51 0.8528718857958353 1103 52-53 0.7276910878606166 1103 54-55 0.7205267563028279 1103 56-57 0.6394691519589593 1103 58-59 0.5980517512325036 1103 60-61 0.6631195483877477 1103 62-63 0.7323681648556608 1103 64-65 0.7499423417985511 1103 66-67 0.7714270999770001 1103 68-69 0.7279771527460994 1103 70-71 0.7466967389959542 1103 72-73 0.7561104714895173 1103 74-75 0.7504951340666572 1103 76-77 0.7148982393413377 1103 78-79 0.7522098100462671 1103 80-81 0.7797985734968087 1103 82-83 0.886944637885204 1103 84-85 0.9527567132984593 1103 86-87 0.9192535944830311 1103 88-89 0.9882190831372313 1103 90-91 1.0394477113542386 1103 92-93 1.0235812514330789 1103 94-95 1.1565904218235836 1103 96-97 1.1541875445800862 1103 98-99 1.3084459220312397 1103 100-101 1.5590180582091229 1104 1 0.309524576037127 1104 2 0.28335259760963183 1104 3 0.39665383667698606 1104 4 0.4973659203042544 1104 5 0.40431906529499884 1104 6 0.31801384789015685 1104 7 0.38538927357710406 1104 8 0.34927258702920483 1104 9 0.44572489790397185 1104 10-11 0.47733978295377 1104 12-13 0.5093665642421428 1104 14-15 0.5006800608249051 1104 16-17 0.6335500194588946 1104 18-19 0.5399786211239785 1104 20-21 0.59223758720983 1104 22-23 0.4946697868745815 1104 24-25 0.5556169909573967 1104 26-27 0.5730270020026609 1104 28-29 0.45891795455276707 1104 30-31 0.5551577896085718 1104 32-33 0.5502765901376279 1104 34-35 0.6535912862683801 1104 36-37 0.822560984174217 1104 38-39 0.6936355993502872 1104 40-41 0.7470800124220318 1104 42-43 0.8122034330046333 1104 44-45 0.7753594701425968 1104 46-47 0.6688213836474475 1104 48-49 0.8060369842771351 1104 50-51 0.542778291454546 1104 52-53 0.6149549081052008 1104 54-55 0.7245112323662433 1104 56-57 0.6510712023935241 1104 58-59 0.6370644286577161 1104 60-61 0.5644599063745517 1104 62-63 0.6656374537292855 1104 64-65 0.7391223584391007 1104 66-67 0.6091855312389107 1104 68-69 0.5844692580834625 1104 70-71 0.6227185728891165 1104 72-73 0.619730666415915 1104 74-75 0.6438158355826502 1104 76-77 0.7181118642942153 1104 78-79 0.716841223690075 1104 80-81 0.6286618140973665 1104 82-83 0.6062497049045916 1104 84-85 0.7148389297784057 1104 86-87 0.7775880754532061 1104 88-89 0.8569864757874299 1104 90-91 0.8850895892883877 1104 92-93 1.0418588468165808 1104 94-95 1.0563593543531766 1104 96-97 0.9082343687583254 1104 98-99 0.9122099253397096 1104 100-101 1.2189721095829285 1105 1 0.0419938692515025 1105 2 0.023834641044420124 1105 3 0.16029256007330162 1105 4 0.21933321446343967 1105 5 0.21977876876313474 1105 6 0.15092357353800878 1105 7 0.19116495171851966 1105 8 0.21373735801636684 1105 9 0.15944698518969602 1105 10-11 0.17066825540422315 1105 12-13 0.17850800296984204 1105 14-15 0.01465900641017015 1105 16-17 0.17918329512281161 1105 18-19 0.08301778137315097 1105 20-21 0.28737126292966053 1105 22-23 0.24881379937098558 1105 24-25 0.31811437199377934 1105 26-27 0.49906786582958773 1105 28-29 0.4619136563914381 1105 30-31 0.4419155317475614 1105 32-33 0.5387289862921634 1105 34-35 0.5546737960281334 1105 36-37 0.5223304685612291 1105 38-39 0.6776083703394633 1105 40-41 0.6351647381365026 1105 42-43 0.616688229475244 1105 44-45 0.6489107574266697 1105 46-47 0.6763731098442705 1105 48-49 0.8101027969870032 1105 50-51 0.7513421818145147 1105 52-53 0.597930546287845 1105 54-55 0.590502942864763 1105 56-57 0.6921362456736766 1105 58-59 0.7545501278019806 1105 60-61 0.6384451871688661 1105 62-63 0.6314161251777541 1105 64-65 0.6694154144349014 1105 66-67 0.7967645277881807 1105 68-69 0.69943232538483 1105 70-71 0.5569894645841948 1105 72-73 0.6711125897078816 1105 74-75 0.57853026753601 1105 76-77 0.4398946876237737 1105 78-79 0.5101215194541595 1105 80-81 0.5237628774632093 1105 82-83 0.5274919863893963 1105 84-85 0.5865231273859344 1105 86-87 0.7118001417504907 1105 88-89 0.7754313368172454 1105 90-91 0.7813652339747748 1105 92-93 0.8994067809629627 1105 94-95 0.9865019347293398 1105 96-97 1.0462405705793891 1105 98-99 1.1518823498010207 1105 100-101 1.4626641795303783 1106 1 0.17423905329463452 1106 2 0.2783576573853601 1106 3 0.2998703944265202 1106 4 0.4731875989942651 1106 5 0.41096454150362405 1106 6 0.36043972527090773 1106 7 0.3389365358355221 1106 8 0.2730023596010298 1106 9 0.13447104008290722 1106 10-11 0.304060818045528 1106 12-13 0.3592037369884622 1106 14-15 0.29552923302647827 1106 16-17 0.3316087988702634 1106 18-19 0.24142502844429004 1106 20-21 0.287129689854531 1106 22-23 0.34120947152424463 1106 24-25 0.43672849738045016 1106 26-27 0.3071145557797479 1106 28-29 0.2274874074953246 1106 30-31 0.43723819041144196 1106 32-33 0.4546823417961505 1106 34-35 0.35821224401365725 1106 36-37 0.45141047001889945 1106 38-39 0.6322430022398393 1106 40-41 0.39085762859976114 1106 42-43 0.3511917254720558 1106 44-45 0.42369267641137043 1106 46-47 0.5561048064581158 1106 48-49 0.4773246738503829 1106 50-51 0.5553399802098156 1106 52-53 0.6841300462851869 1106 54-55 0.38809685508790537 1106 56-57 0.3947510983265694 1106 58-59 0.5879749928047886 1106 60-61 0.46153251279871554 1106 62-63 0.4794038230648958 1106 64-65 0.5431927791357225 1106 66-67 0.705379426536414 1106 68-69 0.7075137400173794 1106 70-71 0.5873960928235924 1106 72-73 0.6316521452996291 1106 74-75 0.5970722611937376 1106 76-77 0.5539782892558627 1106 78-79 0.563826991808341 1106 80-81 0.5394354004824713 1106 82-83 0.5205814789277738 1106 84-85 0.5399561278246985 1106 86-87 0.6979200115108455 1106 88-89 0.6558717938365284 1106 90-91 0.6540587525672095 1106 92-93 0.6804681393841285 1106 94-95 0.6542228453182339 1106 96-97 0.7477143141705263 1106 98-99 0.9093056857493877 1106 100-101 1.1368086932710106 1107 1 0.03292924468440006 1107 2 0.03202341523391539 1107 3 0.1492740015734917 1107 4 0.4201962664928871 1107 5 0.19424246129288036 1107 6 0.27007505888992256 1107 7 0.2565309716245565 1107 8 0.270785667890884 1107 9 0.1573726866594285 1107 10-11 0.19453725502823005 1107 12-13 0.15853447905058715 1107 14-15 0.32287729689049627 1107 16-17 0.23814034770339276 1107 18-19 0.2748390368018292 1107 20-21 0.1950106937843472 1107 22-23 0.2018870104662085 1107 24-25 0.4373375884497648 1107 26-27 0.20513483361658302 1107 28-29 0.20276198263277934 1107 30-31 0.3250106016962562 1107 32-33 0.5050201865546242 1107 34-35 0.39875285952776807 1107 36-37 0.31283236965636263 1107 38-39 0.4430114818182389 1107 40-41 0.2810382025642042 1107 42-43 0.24306015640298284 1107 44-45 0.40456913846524145 1107 46-47 0.23964529061853312 1107 48-49 0.42637712669962724 1107 50-51 0.2873544834206214 1107 52-53 0.4841903331114672 1107 54-55 0.3770939296883178 1107 56-57 0.4359081407856955 1107 58-59 0.6289176915876453 1107 60-61 0.5739840187386527 1107 62-63 0.4532141239631393 1107 64-65 0.5857809229118729 1107 66-67 0.6873760283650121 1107 68-69 0.6395273643072308 1107 70-71 0.4825903225898607 1107 72-73 0.48630181478844037 1107 74-75 0.6167762618389219 1107 76-77 0.6052534363147934 1107 78-79 0.48029031284508505 1107 80-81 0.4777586160745422 1107 82-83 0.42232623045474327 1107 84-85 0.47997492266119934 1107 86-87 0.643055089435375 1107 88-89 0.5809623815204468 1107 90-91 0.5617123904879477 1107 92-93 0.6161040195489527 1107 94-95 0.5836830715843213 1107 96-97 0.6273113879681453 1107 98-99 0.6642201169117499 1107 100-101 0.8173487696674684 1108 1 0.18423059676700504 1108 2 0.14802674608099764 1108 3 0.20666548396497575 1108 4 0.39613327289864486 1108 5 0.16666727043644158 1108 6 0.32143143759005 1108 7 0.3555575854949211 1108 8 0.27454713024609845 1108 9 0.37891981211280523 1108 10-11 0.33900059011656936 1108 12-13 0.22717680238041282 1108 14-15 0.3753464563909077 1108 16-17 0.23048227871719718 1108 18-19 0.3266817429414104 1108 20-21 0.3140909905990199 1108 22-23 0.28766276694508974 1108 24-25 0.4716095277998349 1108 26-27 0.5252436802879288 1108 28-29 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0.12021330190886204 2311 22-23 0.06751175407956111 2311 24-25 0.15908575682355064 2311 26-27 0.04978902386322659 2311 28-29 0.15926259773284102 2311 30-31 0.15552398006551726 2311 32-33 0.0019279698607235218 2311 34-35 0.021694855296139792 2311 36-37 0.2260549868786228 2311 38-39 0.13383851449716389 2311 40-41 0.0778728473057555 2311 42-43 0.11474032534013645 2311 44-45 0.19117319247371256 2311 46-47 0.5207790506096401 2311 48-49 0.28299981602919644 2311 50-51 0.2729839377113237 2311 52-53 0.23409042778081712 2311 54-55 0.2742587149707667 2311 56-57 0.38400043040734744 2311 58-59 0.288896191573059 2311 60-61 0.1672319054289737 2311 62-63 0.06210417716393479 2311 64-65 0.1157101841263426 2311 66-67 0.18344527349890782 2311 68-69 0.18098020260998382 2311 70-71 0.3056044142837564 2311 72-73 0.20618097577282057 2311 74-75 0.1850472859998895 2311 76-77 0.1946588961881197 2311 78-79 0.12473692676370973 2311 80-81 0.20304683454969208 2311 82-83 0.33753003277129423 2311 84-85 0.40650553385961885 2311 86-87 0.41930794273389793 2311 88-89 0.4597383700106228 2311 90-91 0.561359653737096 2311 92-93 0.42814509884330576 2311 94-95 0.45469412834399847 2311 96-97 0.6359201280408548 2311 98-99 0.6541747341959905 2311 100-101 0.6344656209021338 2312 1 -0.02723779409743088 2312 2 -0.23100673602570865 2312 3 -0.04749207235720121 2312 4 0.10524205561779354 2312 5 0.09934807173028304 2312 6 0.02244948243492928 2312 7 0.0033705243821486874 2312 8 0.02525149002314464 2312 9 -0.031085833408610597 2312 10-11 -0.01191700956506736 2312 12-13 -0.05423259972648253 2312 14-15 0.05684901538544551 2312 16-17 -0.07133157641038679 2312 18-19 -0.05449521523313905 2312 20-21 -0.038498342836113864 2312 22-23 0.02557660795888239 2312 24-25 0.0049018212003559825 2312 26-27 -0.0742327496601689 2312 28-29 -0.15376572128874244 2312 30-31 -0.1799800619447396 2312 32-33 -0.1785479683018636 2312 34-35 -0.20520341272690956 2312 36-37 -0.08161097321382726 2312 38-39 -0.13361268126119086 2312 40-41 -0.05282070206295941 2312 42-43 0.027971819301853884 2312 44-45 0.1085754837586208 2312 46-47 0.0793876080127589 2312 48-49 0.056988145296053005 2312 50-51 -0.24145299167038559 2312 52-53 0.06713698755084962 2312 54-55 0.11173252129636069 2312 56-57 0.12455162752136317 2312 58-59 0.09084974573558924 2312 60-61 0.054985769545783825 2312 62-63 0.015697624800960597 2312 64-65 0.15222681265684557 2312 66-67 0.1028040033151747 2312 68-69 0.1832416631447522 2312 70-71 0.23979494357408626 2312 72-73 0.21081716600617284 2312 74-75 0.22421196701898083 2312 76-77 0.21645154295129387 2312 78-79 0.18956167395599266 2312 80-81 0.090301731703196 2312 82-83 -0.037268861823196886 2312 84-85 -0.021707602536700676 2312 86-87 0.007706751720323979 2312 88-89 -0.010294681971757313 2312 90-91 0.0809674488015375 2312 92-93 -0.0235386370373476 2312 94-95 -0.10873239221592712 2312 96-97 0.08366990212266501 2312 98-99 0.08632214967172303 2312 100-101 0.08729341283414982 2313 1 -0.6389906468436592 2313 2 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2313 60-61 -0.3931933205498943 2313 62-63 -0.30878198719604555 2313 64-65 -0.20421141682703237 2313 66-67 -0.202765332186722 2313 68-69 -0.40518909122704017 2313 70-71 -0.3207017224318953 2313 72-73 -0.34307045160451466 2313 74-75 -0.39900736006497084 2313 76-77 -0.48356090584468703 2313 78-79 -0.3995378203109681 2313 80-81 -0.5656647506850128 2313 82-83 -0.41904349351078807 2313 84-85 -0.4347032871971983 2313 86-87 -0.5243978235516131 2313 88-89 -0.5709261865588218 2313 90-91 -0.502136023133442 2313 92-93 -0.478665198767974 2313 94-95 -0.40255840090921424 2313 96-97 -0.3209834398629283 2313 98-99 -0.3137041167828869 2313 100-101 -0.45618265790395895 2314 1 -0.7403483726506757 2314 2 -0.9955230374687858 2314 3 -0.6226600292315077 2314 4 -0.8962344131787177 2314 5 -0.8525765502589877 2314 6 -0.6944671799214674 2314 7 -0.5834537257744543 2314 8 -0.6200995671425389 2314 9 -0.6136245252702466 2314 10-11 -0.7347254956256535 2314 12-13 -0.7115119282438087 2314 14-15 -0.8625054497674469 2314 16-17 -0.8854603270641164 2314 18-19 -2.659769520848563 2314 20-21 -1.2866578573272491 2314 22-23 -0.9915929236009191 2314 24-25 -1.0329258024613068 2314 26-27 -1.1523917756539817 2314 28-29 -0.7484175663718702 2314 30-31 -0.8541174906097595 2314 32-33 -0.8358227279324453 2314 34-35 -0.9370263698023678 2314 36-37 -1.0653488685374697 2314 38-39 -0.9291485915615922 2314 40-41 -0.7713324518603741 2314 42-43 -1.0852240451372666 2314 44-45 -0.9605825479994508 2314 46-47 -0.7916314548769634 2314 48-49 -0.8838871269693698 2314 50-51 -0.954052515816521 2314 52-53 -0.9391828460152389 2314 54-55 -0.8614792049382451 2314 56-57 -0.8322899211875665 2314 58-59 -0.8410893012923353 2314 60-61 -0.7689654554330971 2314 62-63 -0.8468321016980092 2314 64-65 -0.8985353868454631 2314 66-67 -0.9403438159752966 2314 68-69 -0.9644553305852419 2314 70-71 -1.0819137051273735 2314 72-73 -0.9845830569784901 2314 74-75 -0.9723171158129205 2314 76-77 -1.0205645717133969 2314 78-79 -1.0395100949215532 2314 80-81 -0.9289042589981129 2314 82-83 -1.0002848155711206 2314 84-85 -1.0109498857157284 2314 86-87 -1.162306179091214 2314 88-89 -1.0444857304019557 2314 90-91 -1.0198021356309148 2314 92-93 -1.0816369169520605 2314 94-95 -1.0775668375990541 2314 96-97 -1.0438564911004384 2314 98-99 -1.0921832496604686 2314 100-101 -1.0540204725586335 2315 1 -0.9731130051016628 2315 2 -1.811424532631495 2315 3 -1.386414391304875 2315 4 -0.5450445246775715 2315 5 -0.9221495635650854 2315 6 -1.4722518140747312 2315 7 -1.2886450895784662 2315 8 -1.4692837530307017 2315 9 -1.513803405742344 2315 10-11 -0.8681711080181884 2315 12-13 -1.8411793467475093 2315 14-15 -9.047382815216995 2315 16-17 -4.562521353534457 2315 18-19 -1.9298274690427277 2315 20-21 -1.1239351433374054 2315 22-23 -2.0427916071768237 2315 24-25 -1.9374572519463698 2315 26-27 -1.469707575580209 2315 28-29 -1.0676537425003332 2315 30-31 -1.3107154989508913 2315 32-33 -1.621714222244016 2315 34-35 -1.5877750860051592 2315 36-37 -1.4272873313853722 2315 38-39 -1.5447491391259192 2315 40-41 -2.619854666516943 2315 42-43 -2.110641314692124 2315 44-45 -1.7766556889759855 2315 46-47 -1.509193020400268 2315 48-49 -1.9407226732569924 2315 50-51 -1.6238178149909217 2315 52-53 -1.562356662226545 2315 54-55 -1.6740316207466819 2315 56-57 -1.7834183860214523 2315 58-59 -1.9347650751170562 2315 60-61 -1.6534541353207466 2315 62-63 -1.5008837940406217 2315 64-65 -1.4997698890726028 2315 66-67 -1.5563451777933217 2315 68-69 -1.750096165266207 2315 70-71 -1.496478987279044 2315 72-73 -1.4902622637194902 2315 74-75 -1.5666673442011536 2315 76-77 -1.513658026475504 2315 78-79 -1.5959737655885604 2315 80-81 -1.650367433168963 2315 82-83 -1.5283589069831542 2315 84-85 -1.556077130587628 2315 86-87 -1.658555434346983 2315 88-89 -1.6633329372564383 2315 90-91 -1.6028679993923696 2315 92-93 -1.6853116977844742 2315 94-95 -1.7214003553452422 2315 96-97 -1.83273355865229 2315 98-99 -1.8321604108373073 2315 100-101 -2.0334085055827202 2316 1 -0.9715528826103323 2316 2 -1.1154756012817515 2316 3 -1.4433707947927878 2316 4 -1.4638593491167953 2316 5 -0.9147357347354799 2316 6 -0.9401925795343047 2316 7 -1.1170973252896488 2316 8 -1.195378488521854 2316 9 -1.5309821863480408 2316 10-11 -1.384755334517493 2316 12-13 -1.2696649166264393 2316 14-15 -1.3899747304036296 2316 16-17 -2.1062661471660817 2316 18-19 -1.490461644692708 2316 20-21 -1.8509695954153713 2316 22-23 -1.4283599762179264 2316 24-25 -1.29444361471311 2316 26-27 -1.3910281370822588 2316 28-29 -1.7567789290753169 2316 30-31 -1.533557328262873 2316 32-33 -1.7303802937978219 2316 34-35 -2.115827689174452 2316 36-37 -1.7404800527905024 2316 38-39 -1.7042730235053583 2316 40-41 -2.100272897949992 2316 42-43 -2.1928436132323625 2316 44-45 -2.444057157616392 2316 46-47 -2.2839769107914236 2316 48-49 -2.9412492329126145 2316 50-51 -2.38558705096375 2316 52-53 -3.00523502824986 2316 54-55 -4.313875618927671 2316 56-57 -2.91507469011529 2316 58-59 -2.532793603582043 2316 60-61 -2.2656324257107947 2316 62-63 -2.7885762620434953 2316 64-65 -2.8990213466570793 2316 66-67 -2.6766888492632113 2316 68-69 -2.677601105169842 2316 70-71 -2.941373520386133 2316 72-73 -6.093858191721065 2316 74-75 -3.6725249072005717 2316 76-77 -3.0686033537716817 2316 78-79 -2.939289369704106 2316 80-81 -2.8010847448243332 2316 82-83 -2.9599216603481615 2316 84-85 -2.9677003125928323 2316 86-87 -2.756269978941109 2316 88-89 -2.6367046290954583 2316 90-91 -2.522258057381851 2316 92-93 -2.8846375047430115 2316 94-95 -3.172368409575512 2316 96-97 -3.2148715356341064 2316 98-99 -3.511877343332973 2316 100-101 -3.6257367249784096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97841.0 3 16770.0 4 14475.0 5 14253.0 6 15941.0 7 19711.0 8 24430.0 9 28643.0 10 32289.0 11 38369.0 12 39803.0 13 44249.0 14 50819.0 15 59752.0 16 75150.0 17 99317.0 18 143213.0 19 205866.0 20 264077.0 21 193783.0 22 119282.0 23 107476.0 24 113495.0 25 123417.0 26 136872.0 27 156489.0 28 179165.0 29 209761.0 30 247506.0 31 294843.0 32 356888.0 33 442088.0 34 565908.0 35 771883.0 36 1094433.0 37 1246873.0 38 949224.0 39 154250.0 40 153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.664378162949168 45.993226822278054 23.774130689904638 18.56826432486814 2 17.679305200926155 46.72600417318728 19.307854926831947 16.286835699054627 3 42.05295978988238 21.08509440958132 19.756460609982213 17.105485190554088 4 17.368054209882803 20.026382057452324 20.80447867377823 41.80108505888664 5 17.368243087891017 44.65988921225305 21.59221754169068 16.37965015816526 6 41.84722811899751 20.82171663833354 19.887324207928174 17.443731034740768 7 17.79521112247748 20.37238002606573 45.46851328875241 16.36389556270438 8 41.47934955789216 20.94536752592009 20.680060490598937 16.89522242558882 9 41.02055787779346 20.707583683785284 21.315844653170675 16.95601378525058 10-11 16.950639231396355 32.94694693412239 32.946758189760835 17.155655644720426 12-13 16.847059254148032 21.051334836136242 32.8893535383402 29.212252371375524 14-15 16.98729200790805 45.05373440041437 20.72569037285493 17.23328321882265 16-17 29.15215718632615 20.837931854463427 20.808324793971575 29.20158616523885 18-19 17.118925756686185 32.868004636779595 20.79735522172669 29.21571438480753 20-21 29.265614619706948 32.84828636854413 20.812524380912105 17.073574630836823 22-23 29.19614331388165 20.758990622781212 32.93787122279148 17.106994840545656 24-25 17.123360004455257 32.65433172781333 21.135812537990752 29.086495729740662 26-27 17.31507572721564 25.39027445587368 40.00723632070372 17.287413496206963 28-29 26.798030069265703 20.672888900883216 35.311878636129315 17.217202393721763 30-31 24.964794352826676 21.658447823149878 35.64552338299857 17.73123444102488 32-33 20.067255671202577 23.72917820483113 36.816579761312 19.386986362654294 34-35 19.650582259974723 23.003574710745543 37.45387802022917 19.891965009050566 36-37 19.333990155444532 23.193198386141074 37.437619576950674 20.035191881463714 38-39 19.843786818760197 22.992981059536312 37.224068542726016 19.939163578977475 40-41 19.778772146071542 22.951017548979678 37.25387083274415 20.016339472204628 42-43 20.102757733131792 23.049069110401955 37.287581452696884 19.56059170376937 44-45 19.987474149637656 23.23211882826977 37.07839829448076 19.702008727611815 46-47 20.012377527628882 23.094683798137662 37.10533265720441 19.78760601702905 48-49 19.95479138326235 23.03244435212751 37.06813269922805 19.94463156538209 50-51 20.070631087959008 23.291100404013804 36.4533933563026 20.18487515172459 52-53 20.596905731562227 23.868344967575766 35.48803471056547 20.04671459029653 54-55 21.251665463018302 24.518297179952224 34.0538394743795 20.176197882649973 56-57 21.389945486862967 25.129541884732937 32.99287779781198 20.487634830592125 58-59 21.38634820705509 25.766595442251127 32.11889882086607 20.728157529827715 60-61 21.625149086423782 26.015344372985627 31.810449952676013 20.549056587914574 62-63 21.55742236309686 25.92300501526629 31.67727229165442 20.84230032998243 64-65 20.94546915428555 25.731555380103572 32.081476835544855 21.241498630066022 66-67 21.018184579259977 25.431818433912103 32.60513483401882 20.944862152809105 68-69 21.078936792349428 25.25980099633907 32.73086889528766 20.930393316023842 70-71 20.82175771655845 25.27242118702543 33.193519610202706 20.712301486213416 72-73 20.69882014297011 25.15899337177666 33.02311556513307 21.119070920120166 74-75 20.9683154733244 25.38319212230402 32.27930376870175 21.369188635669826 76-77 21.348911824908985 25.594512584732392 31.993878323619885 21.062697266738738 78-79 21.224205942729863 25.67571005896691 31.79102210171405 21.309061896589174 80-81 21.186911316418488 26.05263135325146 31.555461151136104 21.204996179193948 82-83 21.706642829645325 26.26486709658002 31.07220683777094 20.95628323600372 84-85 21.52902684070661 26.648731619120607 30.571068426368818 21.251173113803958 86-87 21.418617313073987 26.662430817222756 30.67522344849149 21.24372842121177 88-89 21.36322309385825 26.50221901077779 30.536020517973867 21.59853737739009 90-91 21.39152067372167 26.391745091329387 30.722158708703944 21.494575526244997 92-93 21.443273458608132 26.635844241858457 30.58597750432583 21.33490479520758 94-95 21.543657710835635 26.38210151895988 30.147588333929775 21.926652436274704 96-97 21.378678261299072 26.230048858258915 30.437419307038688 21.953853573403325 98-99 21.799752079600722 26.379430267899707 30.1466951931777 21.67412245932188 100-101 22.110976334014573 26.268179973556403 29.878647449240077 21.74219624318895 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9762.0 1 6044.0 2 1697.5 3 975.0 4 817.5 5 763.5 6 826.5 7 986.5 8 992.5 9 1001.5 10 1364.5 11 8072.5 12 140342.0 13 158882.5 14 45936.0 15 41235.0 16 43666.5 17 46390.5 18 48634.0 19 50502.5 20 52066.5 21 53657.5 22 56414.0 23 60442.0 24 65883.5 25 72465.0 26 79730.5 27 88178.0 28 96876.0 29 105909.0 30 116460.5 31 127776.0 32 139533.0 33 151464.5 34 167864.5 35 182175.5 36 191015.0 37 200374.0 38 209605.5 39 219323.0 40 229243.5 41 237684.0 42 243098.5 43 251158.0 44 261578.5 45 269187.5 46 274006.5 47 277488.0 48 279976.0 49 281229.0 50 284732.5 51 285103.5 52 282545.0 53 278434.5 54 268435.0 55 255638.0 56 238596.5 57 219386.0 58 211467.5 59 183962.0 60 138068.5 61 109379.0 62 87507.0 63 67727.0 64 50842.0 65 37347.0 66 26700.5 67 18816.0 68 12643.5 69 8179.0 70 5470.0 71 3799.0 72 3074.0 73 2679.0 74 2327.5 75 2112.0 76 1955.0 77 1848.5 78 1695.5 79 1557.5 80 1497.5 81 1423.5 82 1296.5 83 1128.0 84 1036.5 85 942.5 86 774.0 87 644.0 88 539.0 89 421.0 90 325.0 91 258.5 92 193.5 93 134.5 94 91.0 95 66.0 96 40.5 97 23.0 98 13.5 99 4.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.099374116803107 2 0.08272032244123365 3 0.08856115217281724 4 0.19505628056648505 5 0.07583934494923107 6 0.0926303016531377 7 0.09768244791802996 8 0.08645799626164037 9 0.07943985642760451 10-11 0.07742814207778316 12-13 0.07091864592878737 14-15 0.07747386285846092 16-17 0.06755245345138744 18-19 0.05930556763663684 20-21 0.05399624198043219 22-23 0.04389194945064767 24-25 0.0460694016304259 26-27 0.04550932206712336 28-29 0.0443605874525947 30-31 0.03430201570348794 32-33 0.03798825364563217 34-35 0.05204167860645804 36-37 0.04716670036669209 38-39 0.05130443101802919 40-41 0.04901839198414129 42-43 0.046989532341565776 44-45 0.050755781649896095 46-47 0.04430915157433222 48-49 0.0402514322891812 50-51 0.044377732745348855 52-53 0.040274292679520075 54-55 0.04362333986416585 56-57 0.0423945938834511 58-59 0.049498460181257746 60-61 0.048561184177363714 62-63 0.05320755851374087 64-65 0.049807075450832614 66-67 0.04366334554725888 68-69 0.04392052493857127 70-71 0.05283607717073408 72-73 0.040034258580961844 74-75 0.048241138712619404 76-77 0.04678950392610059 78-79 0.045006393479668026 80-81 0.04428629118399334 82-83 0.04362333986416585 84-85 0.04421199491539198 86-87 0.04969848859672294 88-89 0.04165163119743753 90-91 0.04457204606322932 92-93 0.04125157436650715 94-95 0.0441034080612823 96-97 0.030187145442489717 98-99 0.036668066103561915 100-101 0.02533502759306265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8748757.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.480686901818146 #Duplication Level Percentage of deduplicated Percentage of total 1 91.94263281353737 54.688109553108475 2 5.138901269203914 6.113307548257478 3 1.090486693326858 1.9458869272912145 4 0.46991443530973653 1.1180333358921246 5 0.27863829452503847 0.8286798577750202 6 0.1937680255351303 0.6915273155063154 7 0.15791901829517777 0.6575192178140572 8 0.1163576736460143 0.5536827483810012 9 0.0939923565923483 0.503165694027016 >10 0.4956041618596251 4.424740671761807 >50 0.008926946293993147 0.39590544684833234 >100 0.008031260898447403 1.0289710003112764 >500 0.0024705379097158865 1.0340256462964554 >1k 0.0020524468645676456 2.452018474955927 >5k 2.2804965161862733E-4 0.9864366782016594 >10k+ 7.601655053954243E-5 22.577989883571913 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 976134 11.157402131525656 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 22557 0.2578309124370468 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9397 0.10740954400722297 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.7150975847197496E-5 0.0 0.0 0.0 0.0 2 1.257321468638345E-4 0.0 0.0 0.0 0.0 3 2.51464293727669E-4 0.0 0.0 0.0 0.0 4 3.2004546474430595E-4 0.0 0.0 1.1430195169439499E-5 0.0 5 3.4290585508318493E-4 0.0 0.0 1.1430195169439499E-5 0.0 6 3.8862663576094296E-4 0.0 0.0 1.1430195169439499E-5 1.1430195169439499E-5 7 4.2291722126926146E-4 0.0 0.0 2.2860390338878998E-5 1.1430195169439499E-5 8 4.4577761160814045E-4 0.0 0.0 2.2860390338878998E-5 1.1430195169439499E-5 9 5.143587826247774E-4 0.0 0.0 4.5720780677757997E-5 1.1430195169439499E-5 10-11 6.000852463955736E-4 0.0 0.0 4.5720780677757997E-5 1.1430195169439499E-5 12-13 7.486777835982872E-4 0.0 0.0 4.5720780677757997E-5 1.1430195169439499E-5 14-15 8.344042473690834E-4 0.0 0.0 4.5720780677757997E-5 1.1430195169439499E-5 16-17 9.658514918176376E-4 0.0 0.0 4.5720780677757997E-5 2.2860390338878998E-5 18-19 0.0010515779555884338 5.7150975847197496E-6 0.0 6.286607343191725E-5 2.2860390338878998E-5 20-21 0.001120159126605071 1.1430195169439499E-5 0.0 6.8581171016637E-5 2.2860390338878998E-5 22-23 0.0011658799072828288 1.1430195169439499E-5 0.0 6.8581171016637E-5 2.2860390338878998E-5 24-25 0.0012630365662230645 1.1430195169439499E-5 0.0 8.572646377079623E-5 2.2860390338878998E-5 26-27 0.0014173442010104978 1.1430195169439499E-5 0.0 9.144156135551599E-5 2.8575487923598748E-5 28-29 0.0014630649816882559 2.2860390338878998E-5 0.0 9.144156135551599E-5 3.42905855083185E-5 30-31 0.001525931055120173 2.2860390338878998E-5 0.0 9.144156135551599E-5 3.42905855083185E-5 32-33 0.0016745235923228865 2.2860390338878998E-5 0.0 9.144156135551599E-5 3.42905855083185E-5 34-35 0.0017602500560936827 2.2860390338878998E-5 0.0 9.715665894023574E-5 3.42905855083185E-5 36-37 0.0018745520077880777 2.2860390338878998E-5 0.0 1.0287175652495549E-4 3.42905855083185E-5 38-39 0.0020631502280838293 2.2860390338878998E-5 0.0 1.0287175652495549E-4 5.143587826247774E-5 40-41 0.002257463545964301 2.2860390338878998E-5 0.0 1.0858685410967525E-4 6.8581171016637E-5 42-43 0.0023774805952434157 2.2860390338878998E-5 0.0 1.1430195169439499E-4 8.001136618607649E-5 44-45 0.002543218425200288 2.2860390338878998E-5 0.0 1.1430195169439499E-4 8.001136618607649E-5 46-47 0.002640375084140524 2.2860390338878998E-5 0.0 1.2001704927911474E-4 8.001136618607649E-5 48-49 0.0027261015479113204 2.2860390338878998E-5 0.0 1.6002273237215297E-4 8.572646377079623E-5 50-51 0.002937560158545951 2.2860390338878998E-5 0.0 1.7145292754159247E-4 9.144156135551599E-5 52-53 0.0030461470126556265 2.2860390338878998E-5 0.0 1.7145292754159247E-4 9.144156135551599E-5 54-55 0.0031375885740111426 2.2860390338878998E-5 0.0 1.7145292754159247E-4 9.144156135551599E-5 56-57 0.003246175428120818 2.2860390338878998E-5 0.0 1.8288312271103199E-4 9.144156135551599E-5 58-59 0.0034119132580776902 2.2860390338878998E-5 0.0 1.8288312271103199E-4 9.715665894023574E-5 60-61 0.003526215209772085 2.2860390338878998E-5 0.0 1.8859822029575175E-4 1.0287175652495549E-4 62-63 0.003783394601084474 2.2860390338878998E-5 0.0 1.9431331788047148E-4 1.0287175652495549E-4 64-65 0.003989138114134386 2.2860390338878998E-5 0.0 2.1145861063463073E-4 1.0858685410967525E-4 66-67 0.004217742017523176 2.2860390338878998E-5 0.0 2.1717370821935046E-4 1.1430195169439499E-4 68-69 0.004497781799174443 2.2860390338878998E-5 0.0 2.4003409855822948E-4 1.1430195169439499E-4 70-71 0.004709240409809074 2.2860390338878998E-5 0.0 2.4003409855822948E-4 1.1430195169439499E-4 72-73 0.004869263142181226 2.2860390338878998E-5 0.0 2.4003409855822948E-4 1.3144724444855426E-4 74-75 0.0050578613624769776 2.2860390338878998E-5 0.0 2.4003409855822948E-4 1.37162342033274E-4 76-77 0.005337901144128247 2.2860390338878998E-5 0.0 2.4003409855822948E-4 1.37162342033274E-4 78-79 0.005635086218533673 3.42905855083185E-5 0.0 2.4003409855822948E-4 1.37162342033274E-4 80-81 0.006075148732557094 3.42905855083185E-5 0.0 2.4003409855822948E-4 1.37162342033274E-4 82-83 0.0064809206610721955 3.42905855083185E-5 0.0 2.51464293727669E-4 1.37162342033274E-4 84-85 0.007041000224374731 4.5720780677757997E-5 0.0 2.571793913123887E-4 1.37162342033274E-4 86-87 0.007818253495896618 4.5720780677757997E-5 0.0 2.6289448889710846E-4 1.37162342033274E-4 88-89 0.008984133403179446 4.5720780677757997E-5 0.0 2.6289448889710846E-4 1.4287743961799372E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 212970 0.0 91.70896 5 GGTATCA 214035 0.0 91.39173 8 GTATCAA 213965 0.0 91.381 9 TGGTATC 213920 0.0 91.34038 7 AAGCAGT 214820 0.0 91.32944 1 GTGGTAT 215250 0.0 90.82587 6 AGCAGTG 217120 0.0 90.26243 2 CAGTGGT 216395 0.0 90.10799 4 GCAGTGG 217310 0.0 90.080475 3 ATCAACG 213085 0.0 46.33505 10-11 CAACGCA 213350 0.0 46.296932 12-13 TCAACGC 212935 0.0 46.23432 12-13 ACGCAGA 214570 0.0 46.100677 14-15 AACGCAG 214175 0.0 46.04595 14-15 AGAGTAC 210160 0.0 45.79603 18-19 TATCAAC 214415 0.0 45.795017 10-11 CGCAGAG 215655 0.0 45.79369 16-17 CAGAGTA 214650 0.0 45.52294 18-19 GCAGAGT 217485 0.0 45.365776 16-17 GAGTACT 125400 0.0 45.269405 20-21 >>END_MODULE