FastQCFastQC Report
Sat 18 Jun 2016
SRR578592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8748757
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103010.11774244044039628No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90190.10308893023317484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4900.025.204383
ATCAACG64350.019.15381410-11
CGTACGC3200.018.55368820-21
CAACGCA67100.018.4041112-13
ACGTACG3800.016.87409220-21
AGTGGTA81000.016.7711325
TCAACGC61850.016.74107210-11
GTATCAA84600.016.3380059
GGTATCA84050.016.2188688
TCGCGTA3950.015.633409552-53
ACGCAGA81050.015.32445914-15
AACGCAG69250.015.05493112-13
TGGTATC91300.014.9309547
CGCGTAG4550.014.61526654-55
ACTCGCG4300.014.36162150-51
AAGCAGT104250.014.1460511
CGCTAGT4750.013.99948690-91
CTCGCGT4450.013.87684752-53
TATCAAC87000.013.3759079
AGAGTAC90350.013.35294418-19
Produced by FastQC (version 0.11.5)