FastQCFastQC Report
Sat 18 Jun 2016
SRR578591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8821823
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99239911.249364218710804No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT227020.2573391009998727No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG94560.10718872958571035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2143250.091.616125
GGTATCA2151900.091.523428
GTATCAA2155300.091.4217159
TGGTATC2150350.091.394397
AAGCAGT2167250.091.246841
GTGGTAT2165200.090.897446
AGCAGTG2189500.090.255852
GCAGTGG2188950.090.079413
CAGTGGT2186050.089.9265064
ATCAACG2146150.046.34861410-11
CAACGCA2150050.046.30377212-13
TCAACGC2147750.046.26487412-13
ACGCAGA2159400.046.16054514-15
AACGCAG2156650.046.12467214-15
TATCAAC2161150.045.87191410-11
CGCAGAG2170100.045.83962616-17
AGAGTAC2117600.045.77793518-19
CAGAGTA2160250.045.5876818-19
GCAGAGT2187300.045.3727416-17
GAGTACT1269700.045.24538420-21
Produced by FastQC (version 0.11.5)