##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578591_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8821823 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.561139687341267 31.0 31.0 33.0 27.0 34.0 2 30.85376106503157 31.0 31.0 34.0 27.0 34.0 3 30.946852141558498 31.0 31.0 34.0 27.0 34.0 4 34.386608754222344 37.0 35.0 37.0 30.0 37.0 5 34.437735488458564 37.0 35.0 37.0 30.0 37.0 6 34.41773032626023 37.0 35.0 37.0 30.0 37.0 7 34.340130378947755 37.0 35.0 37.0 30.0 37.0 8 34.292839359846596 37.0 35.0 37.0 30.0 37.0 9 35.716859202457364 39.0 35.0 39.0 30.0 39.0 10-11 35.69849978853577 39.0 35.0 39.0 30.0 39.0 12-13 35.735460856559925 39.0 35.0 39.0 30.0 39.0 14-15 36.74942253998975 39.0 36.0 41.0 30.0 41.0 16-17 36.71586059933418 39.5 36.0 41.0 30.0 41.0 18-19 36.70985214733962 40.0 36.0 41.0 30.0 41.0 20-21 36.60633029023593 39.5 36.0 41.0 30.0 41.0 22-23 36.30188731966171 39.0 36.0 40.5 28.5 41.0 24-25 36.18748636194582 39.0 36.0 40.0 29.0 41.0 26-27 36.103457641351454 39.0 36.0 40.0 28.0 41.0 28-29 35.76658435563715 39.0 35.0 40.0 27.0 41.0 30-31 35.49241471972403 38.5 34.0 40.0 25.5 41.0 32-33 35.157257235834365 38.0 34.0 40.0 25.0 41.0 34-35 34.93325007767669 38.0 34.0 40.0 25.0 41.0 36-37 34.97150039169908 38.0 34.0 40.0 25.0 41.0 38-39 34.34553844483164 37.0 33.5 40.0 24.5 41.0 40-41 34.32073200743202 37.0 33.0 40.0 24.0 41.0 42-43 34.148714160327174 37.0 33.0 40.0 23.5 41.0 44-45 33.98267721988981 37.0 33.0 40.0 23.0 41.0 46-47 33.77232971008374 37.0 33.0 40.0 23.0 41.0 48-49 33.42130708131415 37.0 33.0 40.0 20.5 41.0 50-51 32.1572068494233 36.0 31.5 39.0 15.5 40.5 52-53 31.788535884249775 36.0 31.0 39.0 8.0 40.0 54-55 31.517075155554583 37.0 30.5 40.0 2.0 41.0 56-57 31.035721925048826 36.5 29.5 40.0 2.0 41.0 58-59 30.70147712099869 36.0 29.0 40.0 2.0 41.0 60-61 30.38552490794703 36.0 28.5 39.5 2.0 41.0 62-63 30.04588479047925 35.0 28.0 39.0 2.0 41.0 64-65 29.712888367857754 35.0 28.0 39.0 2.0 41.0 66-67 29.407719130161645 35.0 28.0 38.5 2.0 40.0 68-69 29.07528664993619 35.0 26.5 38.0 2.0 40.0 70-71 28.68364101161404 35.0 26.0 37.5 2.0 40.0 72-73 28.28939942458605 34.0 26.0 37.0 2.0 39.0 74-75 27.89224551433417 34.0 26.0 36.0 2.0 39.0 76-77 27.48336857359301 34.0 25.0 36.0 2.0 39.0 78-79 27.091053232421462 34.0 24.5 35.5 2.0 37.5 80-81 26.699489946692424 34.0 23.5 35.0 2.0 37.0 82-83 26.33231470411501 33.0 22.5 35.0 2.0 37.0 84-85 25.931947852501686 33.0 20.0 35.0 2.0 36.0 86-87 25.55564796527883 33.0 19.5 35.0 2.0 36.0 88-89 25.165999816591196 32.5 18.0 35.0 2.0 35.5 90-91 24.77644201204218 32.0 13.0 35.0 2.0 35.0 92-93 24.39691954826117 32.0 6.5 35.0 2.0 35.0 94-95 23.94396322619486 32.0 2.0 35.0 2.0 35.0 96-97 23.44599234194565 31.5 2.0 34.5 2.0 35.0 98-99 22.821891915083764 31.0 2.0 34.0 2.0 35.0 100-101 21.219350014163737 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4346753344888228 1101 2 0.5190913251884517 1101 3 0.5191567453666757 1101 4 0.6486748829202611 1101 5 0.5956833642644312 1101 6 0.392395811561876 1101 7 0.47977929160300903 1101 8 0.6211241350359771 1101 9 0.5952561639722873 1101 10-11 0.59445170480884 1101 12-13 0.6354391193920321 1101 14-15 0.7510221841196412 1101 16-17 0.6762458312426105 1101 18-19 0.6146431014448908 1101 20-21 0.6132953895049127 1101 22-23 0.7401439336151086 1101 24-25 0.8900310961676396 1101 26-27 0.7991953320244676 1101 28-29 0.8203702739903278 1101 30-31 0.9506474972726622 1101 32-33 0.9925701200294625 1101 34-35 1.0115318831669242 1101 36-37 0.8616930040577344 1101 38-39 1.0128143301245416 1101 40-41 0.943218395413318 1101 42-43 1.1342467441566981 1101 44-45 1.0535228851886842 1101 46-47 0.997537246927692 1101 48-49 1.0299464299959027 1101 50-51 1.0662357120716308 1101 52-53 1.0783348955396868 1101 54-55 0.9252090091942158 1101 56-57 0.9750465593413047 1101 58-59 0.938756178347866 1101 60-61 1.031916009313612 1101 62-63 1.009202678319138 1101 64-65 1.121468853212832 1101 66-67 0.9694815639854149 1101 68-69 1.1161888102602475 1101 70-71 1.0424187284994133 1101 72-73 1.1613799959208357 1101 74-75 1.1281297264354642 1101 76-77 0.983719938360494 1101 78-79 1.1091730756361677 1101 80-81 1.1892566943403082 1101 82-83 1.2392747004832465 1101 84-85 1.3409603104324503 1101 86-87 1.384077384184689 1101 88-89 1.5032152728586574 1101 90-91 1.5839534814446559 1101 92-93 1.6396477169266745 1101 94-95 1.5983228848692619 1101 96-97 1.8268465049046725 1101 98-99 2.0648610778674694 1101 100-101 2.409661747385613 1102 1 0.5889265797716554 1102 2 0.5211555335358788 1102 3 0.49959509475359454 1102 4 0.6247775756472862 1102 5 0.576173318860036 1102 6 0.4833815283869001 1102 7 0.531461498536622 1102 8 0.6237232354670823 1102 9 0.572291026976842 1102 10-11 0.6691232130816402 1102 12-13 0.6818633289517777 1102 14-15 0.866884478487215 1102 16-17 0.7862980344164185 1102 18-19 0.6764702371018814 1102 20-21 0.7377958224152792 1102 22-23 0.7671879299224713 1102 24-25 0.8559888601712942 1102 26-27 0.9276366007917716 1102 28-29 0.8345711263773126 1102 30-31 1.0104313124047835 1102 32-33 0.9878740460390603 1102 34-35 1.0964953540513491 1102 36-37 0.8669608309951613 1102 38-39 1.0095083202474697 1102 40-41 1.0060067804733634 1102 42-43 1.075460629725832 1102 44-45 1.0699431711143035 1102 46-47 1.0474341593798613 1102 48-49 1.224961284923836 1102 50-51 1.1515268350746908 1102 52-53 1.1968253694639053 1102 54-55 1.0559012480165642 1102 56-57 0.85699145316973 1102 58-59 0.9771369379601573 1102 60-61 1.1307461274240076 1102 62-63 1.0206111155250142 1102 64-65 1.0545123236385443 1102 66-67 1.0360096884145875 1102 68-69 1.243347320983073 1102 70-71 1.1003234587311361 1102 72-73 1.1483455850762105 1102 74-75 1.2782632037172945 1102 76-77 1.198691674269611 1102 78-79 1.168277831752409 1102 80-81 1.3036916541660624 1102 82-83 1.4045645456430478 1102 84-85 1.4184500364907038 1102 86-87 1.438353367202481 1102 88-89 1.6239505733607054 1102 90-91 1.5604493567140487 1102 92-93 1.5622104152073994 1102 94-95 1.6112254157361399 1102 96-97 1.8258823885455726 1102 98-99 1.9482320775422721 1102 100-101 2.355427130447797 1103 1 0.43955452251942617 1103 2 0.3784894057062296 1103 3 0.2860995168171456 1103 4 0.46613555019283126 1103 5 0.40808816130709147 1103 6 0.2764160608127497 1103 7 0.5519154305678171 1103 8 0.4500211669597931 1103 9 0.7165547964921828 1103 10-11 0.5635885154455806 1103 12-13 0.4442215302129213 1103 14-15 0.7138259620238827 1103 16-17 0.6407866741378996 1103 18-19 0.7003031839534799 1103 20-21 0.7819351390832097 1103 22-23 0.7740059249672981 1103 24-25 0.6378408507560138 1103 26-27 0.833629799229243 1103 28-29 0.7898864803245971 1103 30-31 0.7247452413904512 1103 32-33 0.9198841876327037 1103 34-35 0.8843248386634812 1103 36-37 0.8861110066938522 1103 38-39 0.6658911433518995 1103 40-41 0.7541004435196896 1103 42-43 0.5943052137482496 1103 44-45 0.7501207421107949 1103 46-47 0.825869874342601 1103 48-49 0.8099417797349986 1103 50-51 0.7507895986958886 1103 52-53 0.7271946869644985 1103 54-55 0.7125480357259839 1103 56-57 0.6001281905287428 1103 58-59 0.7675516377599969 1103 60-61 0.6942473838712182 1103 62-63 0.6730240849131697 1103 64-65 0.7139506858824056 1103 66-67 0.6676212238064814 1103 68-69 0.8037152183839495 1103 70-71 0.8398136646453622 1103 72-73 0.885492242930404 1103 74-75 1.027773137146081 1103 76-77 1.0417741778386187 1103 78-79 1.0431148164830226 1103 80-81 1.0322714666496502 1103 82-83 1.1542859009312707 1103 84-85 1.1301290279173344 1103 86-87 1.295950047506384 1103 88-89 1.4243902874016676 1103 90-91 1.138614936470642 1103 92-93 1.2540082819849125 1103 94-95 1.3827203077670234 1103 96-97 1.5489819838183365 1103 98-99 1.6367084049496299 1103 100-101 2.0424396600369477 1104 1 0.20927960141616353 1104 2 0.3169053001159021 1104 3 0.26467245273055795 1104 4 0.2782370082904251 1104 5 0.1851535489156504 1104 6 0.16609339584576333 1104 7 0.3438688110720918 1104 8 0.26586101733052203 1104 9 0.34140858090953685 1104 10-11 0.3914202517466734 1104 12-13 0.3247253174949165 1104 14-15 0.4917688639436548 1104 16-17 0.639378820988469 1104 18-19 0.7266252771876935 1104 20-21 0.46477249722425285 1104 22-23 0.4584782299455483 1104 24-25 0.5110624336083234 1104 26-27 0.6064984176650583 1104 28-29 0.7516343413995017 1104 30-31 0.6998428119439453 1104 32-33 0.8775792630918815 1104 34-35 0.8590242850733816 1104 36-37 0.961310599916132 1104 38-39 0.7937309364299949 1104 40-41 0.8268370263003888 1104 42-43 0.8238992954189044 1104 44-45 1.0880240882394858 1104 46-47 0.9253759939123327 1104 48-49 0.8779242195419243 1104 50-51 0.8839329711314505 1104 52-53 0.7289217734907005 1104 54-55 0.9286317080407507 1104 56-57 0.9829348195255001 1104 58-59 1.0179555150391693 1104 60-61 0.9867223541506682 1104 62-63 0.8685118183209859 1104 64-65 0.9671382691357664 1104 66-67 0.7598135862206021 1104 68-69 0.8386882251969965 1104 70-71 0.9573997050557672 1104 72-73 0.7972636238261757 1104 74-75 0.8672647664531752 1104 76-77 0.9444152191800335 1104 78-79 1.0742812945614268 1104 80-81 0.9349305372676007 1104 82-83 0.985407696721218 1104 84-85 0.996062330933313 1104 86-87 1.075689849417092 1104 88-89 1.16654721193294 1104 90-91 1.268828250085761 1104 92-93 1.2571623311419948 1104 94-95 1.306593474999456 1104 96-97 1.442754017611442 1104 98-99 1.6542274010394067 1104 100-101 1.9413568232397935 1105 1 -0.022411572423770565 1105 2 0.18260026630673565 1105 3 0.20041428665420824 1105 4 0.14355846369452507 1105 5 0.21908578747598284 1105 6 0.2655846483948707 1105 7 0.35842142099659924 1105 8 0.32302677168636507 1105 9 0.3587603751208377 1105 10-11 0.35710806255844574 1105 12-13 0.30455859716290234 1105 14-15 0.1824883373077668 1105 16-17 0.42647838808804295 1105 18-19 0.21928314273812077 1105 20-21 0.15851225608798103 1105 22-23 0.3999493758795083 1105 24-25 0.5737242346172309 1105 26-27 0.583977577623557 1105 28-29 0.706536849359864 1105 30-31 0.6229267803171652 1105 32-33 0.7951614605087869 1105 34-35 0.5509251115196676 1105 36-37 0.9305646516495258 1105 38-39 0.7635939109210668 1105 40-41 0.6570398812966332 1105 42-43 0.9850756397357472 1105 44-45 0.9666295048614302 1105 46-47 0.7122172518279655 1105 48-49 0.7893678172665162 1105 50-51 0.7073668375405191 1105 52-53 0.5534811810253153 1105 54-55 0.7694903535274982 1105 56-57 0.723062185272699 1105 58-59 0.7862006583256367 1105 60-61 0.7460762234218912 1105 62-63 0.7749616770022492 1105 64-65 0.9422525763161893 1105 66-67 0.8279607008139962 1105 68-69 0.9764435827870628 1105 70-71 0.9616600177458317 1105 72-73 0.814588943134968 1105 74-75 0.7575162780269977 1105 76-77 0.71366855537552 1105 78-79 0.8324437932197917 1105 80-81 0.8120469195079458 1105 82-83 0.9745937723535754 1105 84-85 0.9752065475981908 1105 86-87 0.9378772487945746 1105 88-89 0.9082645029064302 1105 90-91 1.0685370261581717 1105 92-93 1.2378642738274088 1105 94-95 1.2743606389649642 1105 96-97 1.39956987393143 1105 98-99 1.638902725714729 1105 100-101 2.0272614420369734 1106 1 0.2559733826269728 1106 2 0.2457096492098252 1106 3 0.2546178677377071 1106 4 0.08419000556013145 1106 5 0.2800642991176048 1106 6 0.1783328759499554 1106 7 0.35429552269638265 1106 8 0.29331894058852725 1106 9 0.35731422492781206 1106 10-11 0.20940629849339842 1106 12-13 0.37706146008164865 1106 14-15 0.27884339723763674 1106 16-17 0.31629283534368824 1106 18-19 0.3205736255662117 1106 20-21 0.27734925522081966 1106 22-23 0.38569307936053576 1106 24-25 0.6095471415258231 1106 26-27 0.4147032021588615 1106 28-29 0.44885426021139807 1106 30-31 0.5182510811322132 1106 32-33 0.35079463948488154 1106 34-35 0.3786513030746761 1106 36-37 0.4299298846197459 1106 38-39 0.4616701921946742 1106 40-41 0.38222803830454666 1106 42-43 0.5999896329689633 1106 44-45 0.5801622462030878 1106 46-47 0.6162917243855048 1106 48-49 0.7219209858947693 1106 50-51 0.5089770180594115 1106 52-53 0.477492302260206 1106 54-55 0.5594495498055814 1106 56-57 0.42879736918390776 1106 58-59 0.5958894800881431 1106 60-61 0.5231077934071777 1106 62-63 0.5884247203996757 1106 64-65 0.6760741533776589 1106 66-67 0.7231264176567223 1106 68-69 0.7231133630651527 1106 70-71 0.7879931534808584 1106 72-73 0.6739360178645661 1106 74-75 0.6593021226744611 1106 76-77 0.6824463431834644 1106 78-79 0.8065412317009049 1106 80-81 0.8356478315076572 1106 82-83 1.0105364489347792 1106 84-85 1.1139075890134649 1106 86-87 1.1608480908657377 1106 88-89 1.1135428002117749 1106 90-91 1.0766369098119917 1106 92-93 1.1614082426509604 1106 94-95 1.2454181405613198 1106 96-97 1.3553835691148706 1106 98-99 1.507178068181009 1106 100-101 1.643721662501033 1107 1 0.1587224347372036 1107 2 0.16070534093229583 1107 3 0.20868695684277938 1107 4 0.1302713969619944 1107 5 0.25374120441382786 1107 6 0.23819113568067962 1107 7 0.25642066475960235 1107 8 0.3188834486272114 1107 9 0.24833747863245037 1107 10-11 0.21047580949358746 1107 12-13 0.35320892548143235 1107 14-15 0.3553565508830374 1107 16-17 0.2542244361127217 1107 18-19 0.3137243575611208 1107 20-21 0.3189571153880095 1107 22-23 0.5369919528868223 1107 24-25 0.5124370543845558 1107 26-27 0.47747040812751607 1107 28-29 0.5625872899268245 1107 30-31 0.5427409247286832 1107 32-33 0.5493461100023822 1107 34-35 0.47403979701903864 1107 36-37 0.4311684285181414 1107 38-39 0.5522642672480274 1107 40-41 0.49973819745657266 1107 42-43 0.4851424290891728 1107 44-45 0.4003174266491456 1107 46-47 0.5158854957293499 1107 48-49 0.5149481039937953 1107 50-51 0.44647229407089384 1107 52-53 0.5651279580322672 1107 54-55 0.7872014534870679 1107 56-57 0.6320827915393998 1107 58-59 0.619714873227057 1107 60-61 0.7087134740119581 1107 62-63 0.7563468800629316 1107 64-65 0.788260119799201 1107 66-67 0.8349801588494898 1107 68-69 0.8292244520355503 1107 70-71 0.7719913724512999 1107 72-73 0.7807462571354407 1107 74-75 0.8794788101518698 1107 76-77 0.9133098951555816 1107 78-79 1.0031203081766087 1107 80-81 0.990952990858954 1107 82-83 0.9771198130742462 1107 84-85 1.0514684855199832 1107 86-87 1.0835131349998157 1107 88-89 1.2059161870126438 1107 90-91 1.2465390218986663 1107 92-93 1.2734265504255156 1107 94-95 1.3223009677808406 1107 96-97 1.464667460554523 1107 98-99 1.6366747899047596 1107 100-101 2.0144534100400904 1108 1 0.18615287075315834 1108 2 0.21051946735957827 1108 3 -0.030496980920265315 1108 4 0.2366473407379246 1108 5 0.23452581404535522 1108 6 0.302861337633189 1108 7 0.3322142749921255 1108 8 0.31313177475498577 1108 9 0.20974528731136388 1108 10-11 0.2992151608755549 1108 12-13 0.3325481248965545 1108 14-15 0.28318904741248474 1108 16-17 0.3661248096476015 1108 18-19 0.17185638726245145 1108 20-21 0.20889631524112673 1108 22-23 0.33221172134140886 1108 24-25 0.4985765266266924 1108 26-27 0.4289528960735609 1108 28-29 0.3537223627640529 1108 30-31 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2315 36-37 -1.6667586146271205 2315 38-39 -1.899192412814628 2315 40-41 -2.0829008057356972 2315 42-43 -2.0640402328154224 2315 44-45 -2.209111850474695 2315 46-47 -2.7439793815381393 2315 48-49 -4.091785339565444 2315 50-51 -3.248664875594251 2315 52-53 -2.6657930879000666 2315 54-55 -2.3574672401104593 2315 56-57 -2.2019846582387785 2315 58-59 -2.585114165395094 2315 60-61 -2.5063109335401386 2315 62-63 -2.2636066132443595 2315 64-65 -1.9563139211287996 2315 66-67 -1.8049743061742518 2315 68-69 -1.7175079625425838 2315 70-71 -1.839519285223826 2315 72-73 -1.8581318998929994 2315 74-75 -2.0279303206004116 2315 76-77 -2.0347662265847823 2315 78-79 -1.964752207882949 2315 80-81 -1.8896010588690864 2315 82-83 -1.9758768905096993 2315 84-85 -1.8014301874486733 2315 86-87 -1.7764456618605742 2315 88-89 -1.9762119809841003 2315 90-91 -2.0442132254546657 2315 92-93 -1.918356737998895 2315 94-95 -2.095878930202076 2315 96-97 -2.1100991018912296 2315 98-99 -2.1739509473636573 2315 100-101 -2.2396166079572737 2316 1 -1.2133667659160707 2316 2 -1.1092672380152315 2316 3 -2.226011096919887 2316 4 -1.3304197136900626 2316 5 -1.1665049650960455 2316 6 -2.676355754880518 2316 7 -6.827143267280359 2316 8 -7.683779675753851 2316 9 -4.2338682211377865 2316 10-11 -2.122148902954997 2316 12-13 -2.1827759946943175 2316 14-15 -2.148379623078867 2316 16-17 -2.0929461609203983 2316 18-19 -1.6283455751296856 2316 20-21 -2.6454744738703155 2316 22-23 -3.2235506475348004 2316 24-25 -2.034795831320352 2316 26-27 -2.3116072469549067 2316 28-29 -3.0845683227328564 2316 30-31 -3.692981236680044 2316 32-33 -3.7020031035695027 2316 34-35 -2.775911565178653 2316 36-37 -3.890407607058812 2316 38-39 -3.2311489788445087 2316 40-41 -4.01303411516448 2316 42-43 -3.4396990535519976 2316 44-45 -3.110205931544293 2316 46-47 -2.772407415667736 2316 48-49 -2.811116096501568 2316 50-51 -2.4089785671270185 2316 52-53 -2.728558450497669 2316 54-55 -4.344677151798805 2316 56-57 -3.1510550742881094 2316 58-59 -2.586137676795129 2316 60-61 -3.348745421898233 2316 62-63 -3.0985123849705154 2316 64-65 -4.550683061936564 2316 66-67 -3.526086730878852 2316 68-69 -3.3964290643325086 2316 70-71 -3.83980935386386 2316 72-73 -3.490949314204329 2316 74-75 -3.1704934553052553 2316 76-77 -3.6834111121873434 2316 78-79 -4.839991442028623 2316 80-81 -3.797947085946195 2316 82-83 -3.361363882968263 2316 84-85 -3.473838191147184 2316 86-87 -4.616666221282731 2316 88-89 -5.056966163079874 2316 90-91 -4.5017212233303745 2316 92-93 -4.60902604643308 2316 94-95 -4.712994916862176 2316 96-97 -4.974516535619962 2316 98-99 -5.0569681042854455 2316 100-101 -5.157126099665593 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99929.0 3 17519.0 4 14935.0 5 14492.0 6 16299.0 7 20057.0 8 24822.0 9 29229.0 10 33628.0 11 40136.0 12 41590.0 13 46520.0 14 52563.0 15 62160.0 16 78080.0 17 103340.0 18 146805.0 19 211817.0 20 268879.0 21 198271.0 22 123106.0 23 111255.0 24 118088.0 25 127757.0 26 141828.0 27 160875.0 28 186573.0 29 217422.0 30 256015.0 31 304008.0 32 368112.0 33 455057.0 34 579968.0 35 789406.0 36 1099495.0 37 1222190.0 38 898630.0 39 140825.0 40 142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.637171034114576 46.052999529692045 23.76151482741151 18.548314608781865 2 17.64300699840314 46.80520263958451 19.29518708858622 16.256603273426133 3 42.09842612026919 21.082937190169485 19.72464452077637 17.093992168784958 4 17.335575117781048 20.03786783743515 20.787214431167808 41.83934261361599 5 17.386603973966796 44.6914116750025 21.548297130719725 16.37368722031098 6 41.91490677275287 20.795431016829067 19.889326502700467 17.40033570771759 7 17.795048610951948 20.37514043882495 45.44393872725833 16.385872222964775 8 41.52823173925159 20.932284580215864 20.620976181810093 16.918507498722455 9 41.122531568938406 20.669993721496287 21.273542403498354 16.93393230606696 10-11 16.95371534050408 32.945539074669824 32.95392815892272 17.14681742590337 12-13 16.83858341581783 21.042145021925414 32.89772519742399 29.22154636483277 14-15 16.991553332394034 45.12071594634373 20.6912897157755 17.196441005486736 16-17 29.16825116595043 20.81212231137766 20.78274349691718 29.23688302575473 18-19 17.103950875749916 32.894654012969546 20.77308220120431 29.22831291007623 20-21 29.277183413802803 32.87179783234305 20.799834316324027 17.05118443753012 22-23 29.202475570520203 20.730839034953465 32.974044978391326 17.09264041613501 24-25 17.113138593004432 32.6654947846525 21.116058847378433 29.10530777496463 26-27 17.275113500459174 25.3605058547117 40.09858328836862 17.265797356460507 28-29 26.771407084046313 20.649618989408864 35.37072372761481 17.208250198930017 30-31 24.932294773573254 21.619842970934535 35.71788972086537 17.729972534626835 32-33 20.017629648657376 23.70829934565027 36.90247243205511 19.37159857363724 34-35 19.623559245839086 22.964289037964893 37.50932402393143 19.90282769226459 36-37 19.334198368198415 23.150545667183565 37.52611180815688 19.98914415646115 38-39 19.810935090553578 22.97943555331473 37.329522517025985 19.880106839105707 40-41 19.739504084315737 22.956883770980717 37.30751136649237 19.996100778211176 42-43 20.089510913647093 23.02307525508121 37.340273707627844 19.547140123643846 44-45 19.965848989660056 23.197952413703433 37.14895144671711 19.687247149919397 46-47 19.988060275647953 23.082374571653315 37.13954575518303 19.7900193975157 48-49 19.929244166315335 23.0311656255254 37.11374263940581 19.925847568753458 50-51 20.06194023452367 23.261713141500366 36.52618442439449 20.15016219958147 52-53 20.596013185387747 23.83096152817857 35.52667354405012 20.046351742383568 54-55 21.25912175943425 24.482129250314337 34.10267585218925 20.156073138062162 56-57 21.406262598838687 25.137656577162247 32.990854724495755 20.46522609950331 58-59 21.39307195225604 25.757784106203385 32.13888190627437 20.710262035266208 60-61 21.628697216531304 26.007744133651766 31.82222344196331 20.541335207853614 62-63 21.533306877205725 25.918341978831393 31.71035689346388 20.837994250499 64-65 20.972233649952177 25.707935348264378 32.08948533479966 21.230345666983784 66-67 21.012337434017173 25.415297792183296 32.638985874162266 20.93337889963727 68-69 21.082905430380904 25.260550697902488 32.74234822540883 20.914195646307768 70-71 20.807069277566008 25.241755767261438 33.23344018429878 20.717734770873776 72-73 20.689458473310605 25.14699137254075 33.04956554809029 21.11398460605836 74-75 20.960571936150505 25.359179633620414 32.32368623917562 21.35656219105346 76-77 21.345012362014586 25.575790228825984 32.02252243159614 21.0566749775633 78-79 21.23297217642457 25.639719276089405 31.81248464748586 21.31482390000016 80-81 21.17805818627538 26.006219144140157 31.583812034369394 21.23191063521507 82-83 21.71363619920832 26.254560468526943 31.091528927939354 20.940274404325386 84-85 21.53169053518384 26.611986115234032 30.578540421107725 21.277782928474405 86-87 21.401770018292876 26.655827451427815 30.692049604606996 21.25035292567231 88-89 21.361462002486693 26.469901618231834 30.576219545560505 21.59241683372097 90-91 21.371753449719705 26.380461986501242 30.76150493903344 21.486279624745613 92-93 21.439030573321432 26.641220036615792 30.56992509518956 21.349824294873216 94-95 21.540537276330955 26.371051469183186 30.154825445013294 21.93358580947256 96-97 21.40987490842277 26.211929186549266 30.42771910245753 21.95047680257043 98-99 21.777705584999627 26.368548290165077 30.15241558547587 21.701330539359425 100-101 22.152830476840965 26.21790251473599 29.8100690803225 21.819197928100547 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10157.0 1 6257.5 2 1768.0 3 1102.0 4 922.0 5 760.5 6 829.0 7 1011.5 8 929.5 9 930.0 10 1312.5 11 7984.0 12 143430.0 13 161903.5 14 45636.5 15 41024.0 16 43793.0 17 46642.0 18 49191.0 19 50846.0 20 52281.5 21 54306.5 22 56997.0 23 61014.0 24 66428.5 25 72899.5 26 80696.5 27 89311.0 28 97649.0 29 106668.5 30 117367.0 31 129164.0 32 141045.0 33 153475.5 34 169579.5 35 183223.0 36 192247.0 37 202143.5 38 211407.5 39 221307.5 40 231225.5 41 239266.0 42 245119.0 43 253589.0 44 264524.5 45 271315.5 46 275425.5 47 278964.0 48 281356.5 49 283089.0 50 286488.0 51 286512.5 52 284581.5 53 280539.0 54 270003.0 55 256963.5 56 240232.5 57 221760.0 58 213071.0 59 184606.5 60 138960.0 61 110854.0 62 89160.5 63 68953.5 64 51803.5 65 37879.5 66 26918.5 67 19100.5 68 12910.5 69 8348.0 70 5636.0 71 4016.0 72 3153.5 73 2704.0 74 2412.0 75 2174.0 76 2010.5 77 1855.5 78 1712.0 79 1617.5 80 1512.0 81 1375.0 82 1267.0 83 1121.5 84 972.0 85 874.5 86 746.5 87 615.0 88 515.5 89 409.0 90 304.0 91 241.5 92 167.0 93 102.0 94 73.0 95 48.0 96 35.5 97 29.0 98 23.0 99 14.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11987318267437468 2 0.10799355189964706 3 0.10362937456351141 4 0.09080889516826623 5 0.09593255271614495 6 0.08624067837225934 7 0.09495769751898218 8 0.09130765829239602 9 0.08638804020438859 10-11 0.0770418993897293 12-13 0.06829654142913545 14-15 0.0765034619261801 16-17 0.06740670267358573 18-19 0.06032766696860728 20-21 0.056927009304086014 22-23 0.04874275985813816 24-25 0.04645298369736051 26-27 0.04304099050729084 28-29 0.0456084870440044 30-31 0.03700482315276559 32-33 0.04863507236542832 34-35 0.05913176902325064 36-37 0.05046575974149561 38-39 0.0525911707818214 40-41 0.05303892404098337 42-43 0.04805129279968551 44-45 0.04785292110258843 46-47 0.04759787177774934 48-49 0.04733148692736184 50-51 0.04864074012820253 52-53 0.04569917124839163 54-55 0.045727510062262645 56-57 0.045863536368843494 58-59 0.04861240131433152 60-61 0.04446359896361557 62-63 0.05339032533298389 64-65 0.04912249996400971 66-67 0.04424822397819589 68-69 0.04218515832838632 70-71 0.0498933157013012 72-73 0.043908158211743764 74-75 0.045971223861553334 76-77 0.04172606954367595 78-79 0.04589754294548871 80-81 0.047733898084330185 82-83 0.03826873425141266 84-85 0.048124973715750134 86-87 0.050516769606463424 88-89 0.04626594752581184 90-91 0.04252522409483845 92-93 0.039646000605543776 94-95 0.04339239179929137 96-97 0.03133139261578927 98-99 0.03222689913411321 100-101 0.02369124839616483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8821823.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.22349497504944 #Duplication Level Percentage of deduplicated Percentage of total 1 91.853302570398 54.39873603219662 2 5.208882996912423 6.169765119865266 3 1.0933322136682502 1.9425286458672384 4 0.4805496547547905 1.138393202545283 5 0.2835791453379604 0.8397274044475757 6 0.19766903629333063 0.7023990706584559 7 0.14357942574950902 0.5952292779577572 8 0.1150117438403826 0.5449117946722057 9 0.10203510691170056 0.5438588077317357 >10 0.5001034367874748 4.465668894201434 >50 0.009346250871554721 0.41411981487606425 >100 0.007788265401666624 0.9931039029408956 >500 0.002353145604851996 0.9782503434200304 >1k 0.0021633757797414636 2.4693959708466573 >5k 2.2772376628857503E-4 0.9868143265256686 >10k+ 7.590792209619167E-5 22.817097391247156 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 992399 11.249364218710804 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 22702 0.2573391009998727 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9456 0.10718872958571035 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.6677627742021124E-5 0.0 0.0 0.0 0.0 2 1.7003288322606336E-4 0.0 0.0 0.0 0.0 3 3.060591898069141E-4 0.0 0.0 1.1335525548404225E-5 1.1335525548404225E-5 4 3.740723430973394E-4 0.0 0.0 2.267105109680845E-5 1.1335525548404225E-5 5 3.8540786864574365E-4 0.0 0.0 3.400657664521267E-5 1.1335525548404225E-5 6 4.760920730329774E-4 0.0 0.0 3.400657664521267E-5 1.1335525548404225E-5 7 5.441052263234027E-4 0.0 0.0 3.400657664521267E-5 1.1335525548404225E-5 8 5.55440751871807E-4 0.0 0.0 3.400657664521267E-5 3.400657664521267E-5 9 5.894473285170197E-4 0.0 0.0 3.400657664521267E-5 3.400657664521267E-5 10-11 6.744637701300513E-4 0.0 0.0 3.967433941941479E-5 3.400657664521267E-5 12-13 7.538124489688809E-4 0.0 0.0 4.53421021936169E-5 3.967433941941479E-5 14-15 8.614999416787211E-4 0.0 0.0 4.53421021936169E-5 4.53421021936169E-5 16-17 9.691874343885612E-4 0.0 0.0 4.53421021936169E-5 5.6677627742021124E-5 18-19 0.0010768749270984013 0.0 0.0 5.100986496781901E-5 5.6677627742021124E-5 20-21 0.0011958979453566458 0.0 0.0 5.6677627742021124E-5 5.6677627742021124E-5 22-23 0.0012242367592276563 1.1335525548404225E-5 0.0 5.6677627742021124E-5 5.6677627742021124E-5 24-25 0.0013545953030343048 1.1335525548404225E-5 0.0 6.234539051622323E-5 5.6677627742021124E-5 26-27 0.0015302959490345702 1.1335525548404225E-5 0.0 7.934867883882958E-5 6.234539051622323E-5 28-29 0.0016436512045186124 1.1335525548404225E-5 0.0 1.0201972993563802E-4 6.801315329042534E-5 30-31 0.0018080163249704737 1.1335525548404225E-5 0.0 1.1335525548404225E-4 6.801315329042534E-5 32-33 0.0019667136826481326 1.1335525548404225E-5 0.0 1.1902301825824437E-4 6.801315329042534E-5 34-35 0.0020347268359385585 1.1335525548404225E-5 0.0 1.2469078103244648E-4 6.801315329042534E-5 36-37 0.0021820886680678133 1.1335525548404225E-5 0.0 1.2469078103244648E-4 6.801315329042534E-5 38-39 0.0023521215512938764 1.1335525548404225E-5 0.0 1.2469078103244648E-4 6.801315329042534E-5 40-41 0.0025561610111651525 1.1335525548404225E-5 0.0 1.2469078103244648E-4 6.801315329042534E-5 42-43 0.002669516266649195 1.1335525548404225E-5 0.0 1.360263065808507E-4 7.368091606462746E-5 44-45 0.0028395491498752583 1.1335525548404225E-5 0.0 1.360263065808507E-4 7.934867883882958E-5 46-47 0.0029132300659398857 1.1335525548404225E-5 0.0 1.4736183212925492E-4 7.934867883882958E-5 48-49 0.003037920846972332 1.1335525548404225E-5 0.0 1.5869735767765915E-4 7.934867883882958E-5 50-51 0.0031456083396821724 1.1335525548404225E-5 0.0 1.6436512045186124E-4 1.0201972993563802E-4 52-53 0.003292970171811427 1.1335525548404225E-5 0.0 1.7570064600026548E-4 1.1335525548404225E-4 54-55 0.003428996478392278 1.1335525548404225E-5 0.0 1.813684087744676E-4 1.1335525548404225E-4 56-57 0.0035310162083279162 1.1335525548404225E-5 0.0 1.813684087744676E-4 1.1335525548404225E-4 58-59 0.003678378040457171 1.1335525548404225E-5 0.0 1.870361715486697E-4 1.1335525548404225E-4 60-61 0.0038710819747800424 1.1335525548404225E-5 0.0 1.9270393432287183E-4 1.1335525548404225E-4 62-63 0.004103460248522329 1.1335525548404225E-5 0.0 2.0403945987127603E-4 1.2469078103244645E-4 64-65 0.004284828657296797 1.1335525548404225E-5 0.0 2.0403945987127603E-4 1.360263065808507E-4 66-67 0.004483200354393871 1.1335525548404225E-5 0.0 2.1537498541968026E-4 1.5302959490345704E-4 68-69 0.004681572051490945 1.1335525548404225E-5 0.0 2.267105109680845E-4 1.5869735767765915E-4 70-71 0.004834601646394402 1.1335525548404225E-5 0.0 2.4371379929069082E-4 1.7570064600026548E-4 72-73 0.004987631241297859 1.1335525548404225E-5 0.0 2.4938156206489296E-4 1.813684087744676E-4 74-75 0.005078315445685093 1.1335525548404225E-5 0.0 2.4938156206489296E-4 2.0970722264547815E-4 76-77 0.00525968385445956 1.1335525548404225E-5 0.0 2.4938156206489296E-4 2.1537498541968026E-4 78-79 0.0054750588398792405 1.1335525548404225E-5 0.0 2.6071708761329714E-4 2.1537498541968026E-4 80-81 0.005866134471299186 1.7003288322606336E-5 0.0 2.663848503874993E-4 2.1537498541968026E-4 82-83 0.006296884442138547 2.267105109680845E-5 0.0 2.833881387101056E-4 2.1537498541968026E-4 84-85 0.006852325194010354 2.267105109680845E-5 0.0 3.0039142703271193E-4 2.2104274819388238E-4 86-87 0.00759480211743083 2.267105109680845E-5 0.0 3.060591898069141E-4 2.267105109680845E-4 88-89 0.008717019146722849 2.267105109680845E-5 0.0 3.287302409037225E-4 2.267105109680845E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 214325 0.0 91.61612 5 GGTATCA 215190 0.0 91.52342 8 GTATCAA 215530 0.0 91.421715 9 TGGTATC 215035 0.0 91.39439 7 AAGCAGT 216725 0.0 91.24684 1 GTGGTAT 216520 0.0 90.89744 6 AGCAGTG 218950 0.0 90.25585 2 GCAGTGG 218895 0.0 90.07941 3 CAGTGGT 218605 0.0 89.926506 4 ATCAACG 214615 0.0 46.348614 10-11 CAACGCA 215005 0.0 46.303772 12-13 TCAACGC 214775 0.0 46.264874 12-13 ACGCAGA 215940 0.0 46.160545 14-15 AACGCAG 215665 0.0 46.124672 14-15 TATCAAC 216115 0.0 45.871914 10-11 CGCAGAG 217010 0.0 45.839626 16-17 AGAGTAC 211760 0.0 45.777935 18-19 CAGAGTA 216025 0.0 45.58768 18-19 GCAGAGT 218730 0.0 45.37274 16-17 GAGTACT 126970 0.0 45.245384 20-21 >>END_MODULE