FastQCFastQC Report
Sat 18 Jun 2016
SRR578591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8821823
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103290.11708464338946722No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91440.10365204561460822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG5000.025.6367243
ATCAACG65750.017.43764110-11
CAACGCA66300.017.40039412-13
TCAACGC62650.016.67124210-11
CGTACGC3900.016.43389320-21
TCGCGTA3850.016.03288752-53
AGTGGTA80400.015.4707575
CGCGTAG3900.015.21717154-55
AACGCAG70000.015.19204812-13
GTATCAA83500.015.123829
GGTATCA83200.014.9501088
ACGTACG4250.014.52197520-21
CGCTAGT4500.014.24278790-91
ACGCAGA81950.014.19330314-15
TGGTATC87400.014.17736157
AAGCAGT103450.013.1925781
CTCGCGT5100.012.56876852-53
AGAGTAC91700.012.21838918-19
TATCAAC87150.012.0390228
CGCAGAG89250.011.99512214-15
Produced by FastQC (version 0.11.5)