FastQCFastQC Report
Sat 18 Jun 2016
SRR578590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8168995
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89531010.959854914833466No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT201150.2462359200856409No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG95870.11735837762172703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1979900.091.5665
GGTATCA1983700.091.411698
GTATCAA1985150.091.3651669
TGGTATC1987700.091.239687
AAGCAGT1996700.091.159161
GTGGTAT2000000.090.8139956
AGCAGTG2024300.089.944832
CAGTGGT2017950.089.851224
GCAGTGG2022600.089.7879263
ATCAACG1976050.046.3000110-11
TCAACGC1975800.046.24672312-13
CAACGCA1980000.046.20136612-13
ACGCAGA1987900.046.0506214-15
AACGCAG1984800.046.04960614-15
TATCAAC1991900.045.82077410-11
CGCAGAG1995700.045.82025516-17
AGAGTAC1956000.045.6453518-19
CAGAGTA1991400.045.48220418-19
GCAGAGT2018350.045.24374416-17
GAGTACT1139900.045.1228220-21
Produced by FastQC (version 0.11.5)