##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578590_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8168995 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96661976167203 31.0 31.0 34.0 27.0 34.0 2 31.318675553112715 33.0 31.0 34.0 28.0 34.0 3 31.447459693634283 34.0 31.0 34.0 28.0 34.0 4 34.828976391832775 37.0 35.0 37.0 32.0 37.0 5 34.86257648584679 37.0 35.0 37.0 32.0 37.0 6 34.90154162170499 37.0 35.0 37.0 32.0 37.0 7 34.84181824079951 37.0 35.0 37.0 32.0 37.0 8 34.88169719775811 37.0 35.0 37.0 32.0 37.0 9 36.37675209741223 39.0 37.0 39.0 32.0 39.0 10-11 36.263887418219745 39.0 37.0 39.0 32.0 39.0 12-13 36.30691321270242 39.0 37.0 39.0 32.0 39.0 14-15 37.55759380437863 40.0 38.0 41.0 32.0 41.0 16-17 37.52967145897384 40.0 38.0 41.0 32.0 41.0 18-19 37.46072852290888 40.0 38.0 41.0 31.5 41.0 20-21 37.39687378924825 40.0 37.5 41.0 31.0 41.0 22-23 37.131635531665765 39.5 37.0 41.0 31.0 41.0 24-25 37.084447107630744 40.0 37.0 41.0 31.0 41.0 26-27 36.960345917704686 40.0 37.0 41.0 30.0 41.0 28-29 36.786336446527386 40.0 36.5 41.0 30.0 41.0 30-31 36.55929695146098 40.0 36.0 41.0 29.5 41.0 32-33 36.28165037437286 39.0 36.0 41.0 29.0 41.0 34-35 36.09523521559261 39.0 35.5 41.0 27.0 41.0 36-37 35.82957071218675 39.0 35.0 40.5 27.0 41.0 38-39 35.01981498336087 38.0 35.0 40.0 26.5 41.0 40-41 34.78902863816173 38.0 34.0 40.0 25.5 41.0 42-43 34.48122467941283 37.5 34.0 40.0 24.0 41.0 44-45 34.19112332667605 37.0 33.0 40.0 24.0 41.0 46-47 34.04991225970882 37.0 33.0 40.0 23.0 41.0 48-49 34.08841118154682 38.0 33.0 40.0 23.0 41.0 50-51 33.23938813770849 37.0 32.5 39.5 20.5 40.5 52-53 32.88407613176406 37.5 33.0 39.5 12.0 40.5 54-55 32.57715514576762 38.0 32.5 40.0 3.5 41.0 56-57 32.09696480411605 38.0 31.5 40.0 2.0 41.0 58-59 31.80246627645139 37.0 31.0 40.0 2.0 41.0 60-61 31.53070236914088 37.0 31.0 40.0 2.0 41.0 62-63 31.198296302054292 36.5 31.0 40.0 2.0 41.0 64-65 30.903423799378015 36.0 30.5 39.5 2.0 41.0 66-67 30.57137995555145 35.5 30.0 39.0 2.0 41.0 68-69 30.2388984201851 35.0 30.0 39.0 2.0 41.0 70-71 29.940332378707538 35.0 29.0 38.5 2.0 40.0 72-73 29.613983054219034 35.0 29.0 37.5 2.0 40.0 74-75 29.247618525901903 35.0 29.0 37.0 2.0 39.0 76-77 28.873548582169533 35.0 29.0 36.5 2.0 39.0 78-79 28.530880420418914 35.0 28.0 36.0 2.0 39.0 80-81 28.209251444027082 35.0 27.5 35.5 2.0 37.0 82-83 27.946800310197276 34.0 27.0 35.0 2.0 37.0 84-85 27.676183300393745 34.0 27.0 35.0 2.0 36.5 86-87 27.426513359354487 34.0 26.0 35.0 2.0 36.0 88-89 27.17296931874729 34.0 26.0 35.0 2.0 36.0 90-91 26.931079955367828 34.0 25.0 35.0 2.0 36.0 92-93 26.64647517840322 34.0 25.0 35.0 2.0 35.0 94-95 26.362925916835547 34.0 24.0 35.0 2.0 35.0 96-97 26.080639111665512 34.0 23.5 35.0 2.0 35.0 98-99 25.760397147996784 34.0 19.5 35.0 2.0 35.0 100-101 24.889528638467766 33.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5896514734881215 1101 2 0.6268630435304878 1101 3 0.5248408996023208 1101 4 0.6143092439836622 1101 5 0.5517887403564856 1101 6 0.542384545871748 1101 7 0.5307355548807848 1101 8 0.49867728870415107 1101 9 0.6600913307689567 1101 10-11 0.6749170193979595 1101 12-13 0.726519811564522 1101 14-15 0.8442951267111596 1101 16-17 0.7912021811090568 1101 18-19 0.8152555713326066 1101 20-21 0.8540095412712532 1101 22-23 0.8820497150124638 1101 24-25 0.8223592790732894 1101 26-27 0.9017427413791239 1101 28-29 1.0026539095887799 1101 30-31 1.061081667977497 1101 32-33 1.0780800528846513 1101 34-35 1.1478432376726815 1101 36-37 1.100795791222204 1101 38-39 1.17130627302015 1101 40-41 1.117705798307334 1101 42-43 1.177667263966626 1101 44-45 1.3172311977440287 1101 46-47 1.1849052730624976 1101 48-49 1.053143347275821 1101 50-51 1.1832243117261356 1101 52-53 0.9638816170227571 1101 54-55 0.9053163046093786 1101 56-57 0.8931123606970388 1101 58-59 0.9397083440708762 1101 60-61 0.8301045207783808 1101 62-63 0.8163563779651781 1101 64-65 0.8762095437464623 1101 66-67 0.9000047328950309 1101 68-69 0.8040825305087864 1101 70-71 0.9213136384006155 1101 72-73 0.832174410536318 1101 74-75 0.8332730598638598 1101 76-77 0.8251711479316661 1101 78-79 0.8340750359275297 1101 80-81 0.8218186180921663 1101 82-83 0.8619714311346378 1101 84-85 0.8547739652183957 1101 86-87 0.8727972747924717 1101 88-89 0.8762614051416726 1101 90-91 0.9543552220291716 1101 92-93 1.0476677378545496 1101 94-95 1.149906685590306 1101 96-97 1.180898437365176 1101 98-99 1.1467866587209912 1101 100-101 1.2454769641481143 1102 1 0.5451690988577376 1102 2 0.474901902068666 1102 3 0.5357995206462256 1102 4 0.5345073592703073 1102 5 0.5838315277776829 1102 6 0.5961246039005346 1102 7 0.5848303323978428 1102 8 0.5446287959929208 1102 9 0.6808800086854419 1102 10-11 0.6756541016370718 1102 12-13 0.6760850282035662 1102 14-15 0.8742629908430075 1102 16-17 0.8156863298924506 1102 18-19 0.9370746595998725 1102 20-21 0.9465444778154009 1102 22-23 0.9074286546972345 1102 24-25 0.9234720596797885 1102 26-27 0.971060512008016 1102 28-29 1.0654881962376805 1102 30-31 1.088519149758973 1102 32-33 1.1733534780059998 1102 34-35 1.1834773277663189 1102 36-37 1.2031292921135517 1102 38-39 1.2053385697795491 1102 40-41 1.217874521416853 1102 42-43 1.258085050570351 1102 44-45 1.3270653459464583 1102 46-47 1.3597544446598775 1102 48-49 1.2848147014715536 1102 50-51 1.297444494891991 1102 52-53 1.1620812104580622 1102 54-55 1.0636836590766094 1102 56-57 0.9837531027930098 1102 58-59 1.099490659767067 1102 60-61 1.153604832909945 1102 62-63 1.125427268147753 1102 64-65 1.105427504816241 1102 66-67 1.091396730453848 1102 68-69 1.0787450747657843 1102 70-71 1.0959055879714334 1102 72-73 1.105183894834152 1102 74-75 1.0211082393830004 1102 76-77 1.1063636292309162 1102 78-79 1.1396723989745716 1102 80-81 1.0978034198375113 1102 82-83 1.1127575775854162 1102 84-85 1.1596324602858523 1102 86-87 1.1400226504471895 1102 88-89 1.1354302261256493 1102 90-91 1.1718982618667724 1102 92-93 1.1428082443597525 1102 94-95 1.2553450375750295 1102 96-97 1.3205145922116337 1102 98-99 1.3889126940298269 1102 100-101 1.4762636267969462 1103 1 0.5060732523217943 1103 2 0.5324992190977049 1103 3 0.4367773008226443 1103 4 0.5485506115907341 1103 5 0.6003807360477822 1103 6 0.6007969814058569 1103 7 0.49915314158338475 1103 8 0.5324230402028292 1103 9 0.6627828358630907 1103 10-11 0.6418329160885534 1103 12-13 0.692258295800336 1103 14-15 0.7515668354360514 1103 16-17 0.8711751848082869 1103 18-19 0.8387780802333182 1103 20-21 0.8455721753021734 1103 22-23 0.95855901950614 1103 24-25 0.8647853968901558 1103 26-27 0.9099429141904949 1103 28-29 0.9562643592003539 1103 30-31 1.0227054705694414 1103 32-33 1.0786852623715006 1103 34-35 1.1259115217154942 1103 36-37 1.1703144167177086 1103 38-39 0.9810833907066652 1103 40-41 1.0252159969793908 1103 42-43 1.002703857682917 1103 44-45 0.9448566056948238 1103 46-47 1.2392407805425663 1103 48-49 1.2148821885883834 1103 50-51 1.1715269891914772 1103 52-53 1.1184351729015916 1103 54-55 1.079080176735843 1103 56-57 1.1038027800087278 1103 58-59 1.094941229853422 1103 60-61 1.1065959714347393 1103 62-63 0.8975857476683871 1103 64-65 0.8493556766225154 1103 66-67 0.839921007630064 1103 68-69 1.0500339147131932 1103 70-71 0.9547730713057838 1103 72-73 1.0442887535675247 1103 74-75 1.08925967566665 1103 76-77 1.1217164812656257 1103 78-79 1.0727839273106383 1103 80-81 1.1729240812255561 1103 82-83 1.1561286768117292 1103 84-85 1.1240460833610406 1103 86-87 1.1646595649844578 1103 88-89 1.1553947112639413 1103 90-91 1.0952492055989467 1103 92-93 1.1539875033668459 1103 94-95 1.090024572944646 1103 96-97 1.107284501874016 1103 98-99 1.1883221442788638 1103 100-101 1.1900744532194878 1104 1 0.32924838343102536 1104 2 0.3212873039466544 1104 3 0.35859501983369313 1104 4 0.3182531964901756 1104 5 0.3979590073676249 1104 6 0.41900212320061314 1104 7 0.39147021837076323 1104 8 0.34577674004756886 1104 9 0.4706970180474883 1104 10-11 0.4790277577438573 1104 12-13 0.5596960786202843 1104 14-15 0.6413108060638564 1104 16-17 0.5905945275349254 1104 18-19 0.6471648069976013 1104 20-21 0.663568342661641 1104 22-23 0.6674247186136384 1104 24-25 0.6468450448476872 1104 26-27 0.7113763822220989 1104 28-29 0.7204103441029588 1104 30-31 0.7908938610247205 1104 32-33 0.7477415657376483 1104 34-35 0.8034659089580956 1104 36-37 0.8075146519989502 1104 38-39 0.8352546709468243 1104 40-41 0.756284149798887 1104 42-43 0.9007317895462279 1104 44-45 0.8923953652856298 1104 46-47 1.04304862050666 1104 48-49 0.9577867968525524 1104 50-51 0.9257567206748973 1104 52-53 0.816810138413949 1104 54-55 0.8257982490228315 1104 56-57 0.7955979078016 1104 58-59 0.895366794246538 1104 60-61 0.9016979745682185 1104 62-63 0.8101070399856596 1104 64-65 0.7147686465524785 1104 66-67 0.7904098276963616 1104 68-69 0.772102663079675 1104 70-71 0.661096888832919 1104 72-73 0.7241015432278566 1104 74-75 0.7848848455288682 1104 76-77 0.787529638007264 1104 78-79 0.7240238645872061 1104 80-81 0.875307138413298 1104 82-83 0.8403319716596052 1104 84-85 0.8243682714848326 1104 86-87 0.853402741668269 1104 88-89 0.8537142736155019 1104 90-91 0.8424047423526844 1104 92-93 0.7131223339175357 1104 94-95 0.5887146431144252 1104 96-97 0.8109724249909966 1104 98-99 1.0496725860662899 1104 100-101 1.005444447434872 1105 1 0.20880495459162773 1105 2 0.16185104890458035 1105 3 0.17835733975602963 1105 4 0.22447591604390738 1105 5 0.16757331311709578 1105 6 0.12045014363525297 1105 7 0.12732084460476045 1105 8 0.14067687844409704 1105 9 0.04291779807498841 1105 10-11 0.10563310065241893 1105 12-13 0.09261804274867558 1105 14-15 -0.03571269484973527 1105 16-17 -0.030148579229127392 1105 18-19 -0.08783802955013442 1105 20-21 -0.008865812747671953 1105 22-23 -0.08129084832781786 1105 24-25 -0.09805007537782018 1105 26-27 -0.39253379164158986 1105 28-29 -0.14070290495593696 1105 30-31 -0.339586909756342 1105 32-33 -0.307554229509968 1105 34-35 -0.1946271985496253 1105 36-37 -0.141190253672562 1105 38-39 -0.12865044350059662 1105 40-41 -0.10385827376899215 1105 42-43 -0.011900478005905768 1105 44-45 -0.02662444030430322 1105 46-47 0.05674406539471022 1105 48-49 0.1032824102961527 1105 50-51 0.09243590256428291 1105 52-53 -0.11409194625803565 1105 54-55 -0.18805637749962756 1105 56-57 -0.10700565431241671 1105 58-59 -0.14229866010166248 1105 60-61 -0.17009722956010975 1105 62-63 -0.12034318431971514 1105 64-65 -0.21726728230223102 1105 66-67 -0.21018782834907412 1105 68-69 -0.14978307435336902 1105 70-71 -0.17476026164834124 1105 72-73 -0.15055754298007074 1105 74-75 -0.12360246274794306 1105 76-77 -0.1317240655966856 1105 78-79 -0.11234471438799076 1105 80-81 -0.018887556373790915 1105 82-83 -0.03185047529057883 1105 84-85 -0.06967176614582726 1105 86-87 -0.05274789870160035 1105 88-89 0.020394542879436983 1105 90-91 0.007528291223231065 1105 92-93 0.06651856082785557 1105 94-95 0.11902432113532413 1105 96-97 0.1312667710308233 1105 98-99 0.10354308325767292 1105 100-101 0.03073884159414675 1106 1 -0.159585747761259 1106 2 -0.11897079976035485 1106 3 -0.17917858548181087 1106 4 -0.12203527075646292 1106 5 -0.2067859990159988 1106 6 -0.24918365976822798 1106 7 -0.260751645970295 1106 8 -0.25277020607074974 1106 9 -0.20294898882533374 1106 10-11 -0.41094521283512364 1106 12-13 -0.4324842531890951 1106 14-15 -0.4338803198547083 1106 16-17 -0.42031299287275914 1106 18-19 -0.4242009591047946 1106 20-21 -0.24731087601520585 1106 22-23 -0.18425986371556036 1106 24-25 -0.3174651857066877 1106 26-27 -0.3235550149647466 1106 28-29 -0.330269104087499 1106 30-31 -0.36078911506618994 1106 32-33 -0.30062880923538415 1106 34-35 -0.30237941762115383 1106 36-37 -0.4238953706649795 1106 38-39 -0.46994568050939023 1106 40-41 -0.3912494478216999 1106 42-43 -0.45397454031011364 1106 44-45 -0.46537077806672755 1106 46-47 -0.5231230664530884 1106 48-49 -0.7062084888850322 1106 50-51 -0.7821050502150371 1106 52-53 -0.8204390136613071 1106 54-55 -0.7707715328255844 1106 56-57 -0.8130750322118772 1106 58-59 -0.7750851702110673 1106 60-61 -0.9604390625439692 1106 62-63 -0.7746682914254492 1106 64-65 -0.7310435426732909 1106 66-67 -0.893693624100333 1106 68-69 -0.9722793306321371 1106 70-71 -0.8721070859587208 1106 72-73 -0.9561322245600543 1106 74-75 -1.0437128323577944 1106 76-77 -1.0803436306369392 1106 78-79 -0.9510146499072611 1106 80-81 -0.9419314054443326 1106 82-83 -1.0515748839908632 1106 84-85 -1.1113211175359403 1106 86-87 -1.0226865415098523 1106 88-89 -1.1171695575953393 1106 90-91 -1.0658819513626554 1106 92-93 -1.04554602221123 1106 94-95 -1.0562287308802212 1106 96-97 -1.0585554452159336 1106 98-99 -1.0215874829812925 1106 100-101 -1.1325234493097405 1107 1 -0.4543933114434573 1107 2 -0.5676511286797137 1107 3 -0.4886864973772269 1107 4 -0.4143300592172494 1107 5 -0.5068464541299207 1107 6 -0.4817911517603335 1107 7 -0.3916785257539246 1107 8 -0.3868908360492682 1107 9 -0.45476913857535806 1107 10-11 -0.43793530725208285 1107 12-13 -0.5182190814093985 1107 14-15 -0.7717145481865231 1107 16-17 -0.6351270888009779 1107 18-19 -0.6286501445235544 1107 20-21 -0.5817757325487278 1107 22-23 -0.636622828610264 1107 24-25 -0.5950644736890496 1107 26-27 -0.476601835895039 1107 28-29 -0.5484842659486446 1107 30-31 -0.6185471270786636 1107 32-33 -0.5505392854856765 1107 34-35 -0.5601145269978076 1107 36-37 -0.7371900008113741 1107 38-39 -0.6855480787885924 1107 40-41 -0.8157157704426012 1107 42-43 -0.7216366154671405 1107 44-45 -0.6533422156790891 1107 46-47 -0.7266386523065549 1107 48-49 -0.7504894145158048 1107 50-51 -0.7876782108312383 1107 52-53 -0.8246872197175179 1107 54-55 -0.6761460693061423 1107 56-57 -0.7131681638774836 1107 58-59 -0.6850068604792803 1107 60-61 -0.6068235422617825 1107 62-63 -0.622796580408199 1107 64-65 -0.6143654669761602 1107 66-67 -0.7556171536640868 1107 68-69 -0.6797117268560484 1107 70-71 -0.6536813755149105 1107 72-73 -0.765272437916277 1107 74-75 -0.8123369407913401 1107 76-77 -0.7588650588994845 1107 78-79 -0.7232882476996636 1107 80-81 -0.6163712401894088 1107 82-83 -0.6454894005431235 1107 84-85 -0.7733330946645331 1107 86-87 -0.7807367842912711 1107 88-89 -0.92864833415112 1107 90-91 -0.8239565584546753 1107 92-93 -0.7916825001518397 1107 94-95 -0.8054030838111998 1107 96-97 -0.8641716924499647 1107 98-99 -0.7967367129281193 1107 100-101 -0.9127828903927622 1108 1 -0.7932316791456167 1108 2 -0.782105182151156 1108 3 -0.7013277765537467 1108 4 -0.709148385982239 1108 5 -0.8977653901944507 1108 6 -0.7607189466022177 1108 7 -0.6203420441753948 1108 8 -0.7127515020566477 1108 9 -0.7930895694270035 1108 10-11 -1.0539505383578316 1108 12-13 -0.9725809509296184 1108 14-15 -0.9852159638506208 1108 16-17 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-0.3027426150902457 2305 72-73 -0.2605299360470035 2305 74-75 -0.25167561561314855 2305 76-77 -0.34350909031054755 2305 78-79 -0.3160701077860111 2305 80-81 -0.24360708479813553 2305 82-83 -0.255988128316158 2305 84-85 -0.22125548514375382 2305 86-87 -0.11120990069450798 2305 88-89 -0.12740704249178236 2305 90-91 -0.12520297942351277 2305 92-93 -0.1541467957682272 2305 94-95 -0.07886940491389183 2305 96-97 -0.11684712291598132 2305 98-99 -0.13694393572923858 2305 100-101 -0.08482024139485489 2306 1 -0.3462530490257585 2306 2 -0.3564792916331001 2306 3 -0.36715581772554273 2306 4 -0.463744378445881 2306 5 -0.43320159236864697 2306 6 -0.35701074543086264 2306 7 -0.42060652848532243 2306 8 -0.3890681750642244 2306 9 -0.5363332367131903 2306 10-11 -0.7559891963341059 2306 12-13 -0.7328746133912247 2306 14-15 -0.7906470493942521 2306 16-17 -0.6454492571677122 2306 18-19 -0.6473401110033095 2306 20-21 -0.6641186856769394 2306 22-23 -0.6238408127217738 2306 24-25 -0.6687758319840569 2306 26-27 -0.7086985569597246 2306 28-29 -0.5943603306440579 2306 30-31 -0.6203133464324893 2306 32-33 -0.7060292459707469 2306 34-35 -0.6313768337441843 2306 36-37 -0.6580664648436994 2306 38-39 -0.6730052857528506 2306 40-41 -0.7434088045082348 2306 42-43 -0.8075962781837944 2306 44-45 -0.8812045809290936 2306 46-47 -0.765163467225463 2306 48-49 -0.951403482825171 2306 50-51 -0.8576448435342172 2306 52-53 -0.9100353048954943 2306 54-55 -0.7846238222931667 2306 56-57 -0.9485396788766067 2306 58-59 -0.8941206291903718 2306 60-61 -0.7935401257337737 2306 62-63 -0.9662275900077617 2306 64-65 -0.9976287020343797 2306 66-67 -0.9402571759554128 2306 68-69 -0.9144562983256073 2306 70-71 -0.9096784507035487 2306 72-73 -0.9606139708852588 2306 74-75 -0.9957070285387637 2306 76-77 -0.9749398558436013 2306 78-79 -1.0752467223350486 2306 80-81 -1.1707455951986248 2306 82-83 -1.2222744565727162 2306 84-85 -1.1747681172454172 2306 86-87 -1.183883158259711 2306 88-89 -1.2332002691959332 2306 90-91 -1.2073017392969376 2306 92-93 -1.1735040734603217 2306 94-95 -1.2520419991837848 2306 96-97 -1.1624511239348223 2306 98-99 -1.1690284855578348 2306 100-101 -1.2928269796545209 2307 1 -0.6520037905023663 2307 2 -0.6806852302088124 2307 3 -0.6517511299256888 2307 4 -0.8048156656040675 2307 5 -0.7698714997962384 2307 6 -0.6334732330916069 2307 7 -0.6360841137465059 2307 8 -0.6388840470105066 2307 9 -0.7697607230782992 2307 10-11 -0.8370401829371303 2307 12-13 -0.7968294254697597 2307 14-15 -1.0178579862327837 2307 16-17 -0.9143926639638309 2307 18-19 -0.9707395320351182 2307 20-21 -0.8509722653488794 2307 22-23 -0.8693336648299166 2307 24-25 -0.926310851613998 2307 26-27 -1.0541858228546417 2307 28-29 -0.9589915114009031 2307 30-31 -0.9107955269219872 2307 32-33 -1.1530657147104293 2307 34-35 -0.971353536206017 2307 36-37 -1.0413611796190239 2307 38-39 -1.0579362716999725 2307 40-41 -1.1356165598556274 2307 42-43 -1.3321912260277031 2307 44-45 -1.238949163649302 2307 46-47 -1.2295450537618606 2307 48-49 -1.2660040020284455 2307 50-51 -1.2150003427169835 2307 52-53 -1.1918801949185962 2307 54-55 -1.2059453249122924 2307 56-57 -1.1639596442997515 2307 58-59 -1.2067155784322843 2307 60-61 -1.1628021170661746 2307 62-63 -1.1857391624542686 2307 64-65 -1.1866505435747108 2307 66-67 -1.1623449887808057 2307 68-69 -1.0904036383925124 2307 70-71 -1.0950986700984302 2307 72-73 -1.1266182751819613 2307 74-75 -1.0991566681129648 2307 76-77 -1.1046653750141147 2307 78-79 -1.1016085343756323 2307 80-81 -1.0577427883157924 2307 82-83 -1.0946729828492785 2307 84-85 -1.0789801758992574 2307 86-87 -1.203134518894366 2307 88-89 -1.2145031436288427 2307 90-91 -1.2350689528795087 2307 92-93 -1.109923772125768 2307 94-95 -1.073598058682954 2307 96-97 -1.2034450167009076 2307 98-99 -1.334448778639473 2307 100-101 -1.4032650054681284 2308 1 -1.6377713379094523 2308 2 -1.2373092092133113 2308 3 -1.180024255865174 2308 4 -1.13030467170843 2308 5 -1.4630696190952648 2308 6 -1.5136397185852957 2308 7 -1.3786054361076552 2308 8 -1.343682807407376 2308 9 -1.4096477888692434 2308 10-11 -1.4664203855106805 2308 12-13 -1.338578306776526 2308 14-15 -1.928826010887711 2308 16-17 -2.018099186606385 2308 18-19 -1.7793888075899034 2308 20-21 -2.9700095275087577 2308 22-23 -3.16108409982278 2308 24-25 -2.3942347745787487 2308 26-27 -2.744464752257734 2308 28-29 -2.3171818957411645 2308 30-31 -2.160308819231055 2308 32-33 -2.4883860952737393 2308 34-35 -2.6265196652154543 2308 36-37 -2.991596685458461 2308 38-39 -3.0548851733552382 2308 40-41 -2.658895006795291 2308 42-43 -2.694178884835722 2308 44-45 -2.722092498849104 2308 46-47 -2.8161724768537937 2308 48-49 -2.4860500739239058 2308 50-51 -2.4617161664795653 2308 52-53 -2.419368116311702 2308 54-55 -2.0869286924493196 2308 56-57 -2.036827757532624 2308 58-59 -1.910059853131866 2308 60-61 -1.9036705115469168 2308 62-63 -1.9584034537677937 2308 64-65 -1.9716242037780987 2308 66-67 -2.1829928945796695 2308 68-69 -1.9222916658844618 2308 70-71 -1.8668513965820068 2308 72-73 -1.79984915877316 2308 74-75 -1.5926311622611102 2308 76-77 -1.5523914530049865 2308 78-79 -1.7051457444866749 2308 80-81 -2.3248792029681056 2308 82-83 -2.1453679929562313 2308 84-85 -1.848925824862377 2308 86-87 -1.748469136417885 2308 88-89 -1.6918611613063774 2308 90-91 -1.9450682324887225 2308 92-93 -1.9611781306363305 2308 94-95 -2.0553072752473405 2308 96-97 -2.0480687040092285 2308 98-99 -2.0813521595046574 2308 100-101 -2.253946158721998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84467.0 3 13777.0 4 12132.0 5 11701.0 6 13154.0 7 16301.0 8 19597.0 9 22519.0 10 25730.0 11 30195.0 12 29588.0 13 32370.0 14 35911.0 15 41764.0 16 52742.0 17 70778.0 18 109526.0 19 176164.0 20 248629.0 21 178061.0 22 97289.0 23 83478.0 24 86982.0 25 92499.0 26 100282.0 27 111736.0 28 129165.0 29 151441.0 30 177753.0 31 213135.0 32 259801.0 33 329353.0 34 434958.0 35 632000.0 36 1024516.0 37 1413690.0 38 1334466.0 39 270996.0 40 349.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.710542769662492 45.83895861868018 23.819761754493786 18.630736857163537 2 17.693263780435416 46.612334897840604 19.353934380874406 16.34046694084957 3 41.94402438646385 21.077455152284923 19.797462287587233 17.181058173663995 4 17.386600513419292 20.03632320845536 20.861103534249654 41.715972743875696 5 17.409112037835445 44.496036672995935 21.612782130833775 16.48206915833485 6 41.68726617825228 20.820753584057943 19.98125080258831 17.510729435101467 7 17.847139311110727 20.380396893628987 45.36887451892173 16.403589276338558 8 41.314081321005425 20.964475282442475 20.74428975661089 16.977153639941207 9 40.876257258049904 20.69658915115891 21.42451211850093 17.00264147229025 10-11 16.967415128247097 32.84458086547341 32.974303813445765 17.213700192833727 12-13 16.84237210700647 21.06538157263955 32.88421524586263 29.20803107449135 14-15 16.997386088304197 44.918004367395234 20.80312178223222 17.281487762068355 16-17 29.088521866507005 20.824171001521265 20.875071598091434 29.2122355338803 18-19 17.17001331690607 32.80109982452434 20.83783335645955 29.191053502110037 20-21 29.18274219735147 32.7952332901905 20.870734432375905 17.151290080082124 22-23 29.089190502002456 20.73778266130197 32.98541283815181 17.187613998543757 24-25 17.114911327635028 32.60184133264609 21.19097621283008 29.092271126888797 26-27 17.29760922664513 25.542012395115453 39.80788391236332 17.352494465876095 28-29 27.108075298920394 20.688785595485193 34.91798820532448 17.285150900269933 30-31 25.257873909594153 21.672873073157003 35.27367164568657 17.795581371562275 32-33 20.14821060661954 23.84042029219425 36.49805388883451 19.513315212351696 34-35 19.755292652095576 23.094378375810923 37.11449252262701 20.035836449466494 36-37 19.468226358432577 23.25846195071603 37.0940466199847 20.17926507086669 38-39 19.965199109558473 23.060976294249926 36.899520753250506 20.074303842941088 40-41 19.974902992555858 22.99268929229034 36.821218741824744 20.211188973329058 42-43 20.337070460835815 23.11255311388561 36.81916584581826 19.73121057946032 44-45 20.1968799491978 23.32041345646236 36.60854915342418 19.874157440915663 46-47 20.27220815837343 23.139701077091757 36.623139566419965 19.96495119811485 48-49 20.11866397928063 23.111393432593765 36.64933829474302 20.120604293382595 50-51 20.236324013536933 23.369296792094083 36.003053393809516 20.39132580055947 52-53 20.862760374037332 24.00633654939119 34.86368347357047 20.26721960300101 54-55 21.58540023757191 24.74434663433662 33.289997094708816 20.380256033382654 56-57 21.744813124287987 25.41211349507875 32.132604870821375 20.710468509811893 58-59 21.690314191903965 26.034071237274826 31.347093598190355 20.928520972630853 60-61 21.890722761755185 26.256117539102252 31.111430141428876 20.741729557713686 62-63 21.782693725526336 26.123858304231284 31.030748087696352 21.062699882546028 64-65 21.145310985002816 25.931239843592163 31.459126101751373 21.46432306965365 66-67 21.223512725584445 25.60090688495705 32.04801175842189 21.127568631036617 68-69 21.329812809765773 25.412167564418564 32.12031560905395 21.137704016761713 70-71 21.01330720244787 25.44452074123713 32.6399211170831 20.902250939231905 72-73 20.89497070427396 25.33884213250924 32.41615471045469 21.350032452762115 74-75 21.17462754881586 25.563426807660818 31.66771589685772 21.5942297466656 76-77 21.601444379894858 25.81990248756426 31.299790524710502 21.278862607830376 78-79 21.45535577080267 25.901858684925966 31.11612365913336 21.526661885138004 80-81 21.443109904565624 26.248673143530215 30.903254075078916 21.404962876825245 82-83 21.99104610776535 26.48026614114942 30.421014316913368 21.107673434171854 84-85 21.773242200163278 26.881515235415304 29.896125148899095 21.449117415522323 86-87 21.662468745398105 26.92012383627721 30.0019665403787 21.415440877945986 88-89 21.593986873601516 26.730516639758665 29.902063698489194 21.773432788150625 90-91 21.616455863847236 26.618751948515733 30.096445361856876 21.668346825780155 92-93 21.64268053378925 26.919282418578454 29.924036778294376 21.514000269337913 94-95 21.795996459170112 26.63688593329312 29.437948427469387 22.129169180067386 96-97 21.63938249103081 26.479091994928062 29.71344443172074 22.16808108232039 98-99 22.057097612987278 26.655802827384967 29.481257088443673 21.805842471184082 100-101 22.240216392546845 26.630830042238735 29.25353433253991 21.87541923267451 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7945.0 1 4728.5 2 1110.0 3 611.0 4 512.0 5 525.5 6 598.0 7 734.5 8 740.5 9 741.5 10 1242.5 11 6771.0 12 116377.0 13 130735.5 14 37156.5 15 34738.0 16 37346.5 17 40472.5 18 43605.5 19 45746.0 20 47448.0 21 49480.5 22 52008.0 23 55638.0 24 60392.0 25 66525.0 26 73608.5 27 81435.5 28 89489.5 29 98064.5 30 108051.0 31 118944.5 32 130151.5 33 141580.0 34 156277.0 35 168128.5 36 176580.5 37 185911.5 38 195034.5 39 204421.0 40 213432.5 41 221706.5 42 226963.0 43 234323.5 44 244179.0 45 251722.0 46 256325.0 47 260702.0 48 263646.5 49 265227.5 50 269612.5 51 271259.0 52 270278.5 53 266718.5 54 257683.0 55 246191.5 56 230541.0 57 213283.5 58 207762.0 59 179555.0 60 132692.0 61 105387.5 62 84465.5 63 65114.5 64 48578.5 65 34901.0 66 24183.0 67 16998.5 68 11097.0 69 6724.5 70 4243.5 71 2783.5 72 2032.5 73 1612.0 74 1375.0 75 1202.0 76 1058.0 77 926.5 78 827.0 79 724.5 80 625.5 81 532.5 82 429.0 83 374.5 84 300.0 85 235.0 86 196.5 87 158.5 88 123.5 89 93.5 90 70.5 91 55.5 92 40.5 93 26.0 94 19.0 95 14.0 96 12.5 97 10.0 98 3.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05170770700679827 2 0.09221452577703868 3 0.08277640027934893 4 0.05530668093198735 5 0.0846248528735787 6 0.0013710376857863176 7 0.0012975892383334793 8 0.005251563992877949 9 0.010074678708947673 10-11 0.010013471669403641 12-13 0.017082884736739343 14-15 0.011855803559679006 16-17 0.001615865843962446 18-19 6.793981389387557E-4 20-21 7.344844745283845E-5 22-23 3.7948364517299864E-4 24-25 9.119848892060773E-4 26-27 0.001615865843962446 28-29 0.001707676403278494 30-31 0.005135270617744288 32-33 1.4077619095127368E-4 34-35 0.0026563855162109904 36-37 3.7336294121859543E-4 38-39 0.002919575786250328 40-41 0.003574491109371471 42-43 0.0013404341660143016 44-45 7.038809547563684E-4 46-47 0.0012180200869262375 48-49 0.0026257819964389745 50-51 0.004645614301392032 52-53 0.12989970002429918 54-55 0.14034774167446545 56-57 0.12670469256010072 58-59 0.12027795340797737 60-61 0.10834870140084552 62-63 0.11265767698474535 64-65 0.11543035587609002 66-67 0.12335666749704217 68-69 0.13609385242615524 70-71 0.1232403741219085 72-73 0.11559561488285891 74-75 0.12043709171079184 76-77 0.13163797994734971 78-79 0.14580128889783872 80-81 0.13570212737307344 82-83 0.11525285546141231 84-85 0.12175304306098853 86-87 0.12237123416038326 88-89 0.11888855361032782 90-91 0.12904280147068276 92-93 0.14635215225373502 94-95 0.15564950156047347 96-97 0.15596777816610244 98-99 0.13895222117286155 100-101 0.128394006851516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8168995.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.64611064106219 #Duplication Level Percentage of deduplicated Percentage of total 1 91.72264430413132 55.62621634759141 2 5.404691828853432 6.5554707726698 3 1.1027055753225752 2.0062441297658706 4 0.48031610622621923 1.1651721488351787 5 0.26926123213619063 0.8164823237740074 6 0.18747768624069788 0.6821875501490223 7 0.15290680474086324 0.649124209865998 8 0.1063324565277389 0.5158919938653751 9 0.09156458275543998 0.4997732234930196 >10 0.4598770494034137 4.101631596778889 >50 0.009927669564041083 0.4467844578700535 >100 0.00832654804694591 1.1107613633842561 >500 0.0016108356302147735 0.6521226031023594 >1k 0.002043011031458739 2.3170378943340024 >5k 2.3573204209139292E-4 1.0432198400685455 >10k+ 7.857734736379764E-5 21.811879544452147 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 895310 10.959854914833466 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 20115 0.2462359200856409 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9587 0.11735837762172703 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.344844745283845E-5 0.0 0.0 0.0 0.0 2 1.1017267117925767E-4 0.0 0.0 0.0 1.2241407908806408E-5 3 2.4482815817612817E-4 0.0 0.0 0.0 2.4482815817612816E-5 4 3.3051801353777303E-4 0.0 0.0 0.0 2.4482815817612816E-5 5 3.550008293553858E-4 0.0 0.0 0.0 2.4482815817612816E-5 6 3.9172505308180506E-4 0.0 0.0 0.0 3.672422372641922E-5 7 3.9172505308180506E-4 0.0 0.0 0.0 3.672422372641922E-5 8 4.1620786889941784E-4 0.0 0.0 0.0 3.672422372641922E-5 9 5.263805400786755E-4 0.0 0.0 2.4482815817612816E-5 3.672422372641922E-5 10-11 6.365532112579332E-4 0.0 0.0 2.4482815817612816E-5 3.672422372641922E-5 12-13 7.22243066619578E-4 0.0 0.0 2.4482815817612816E-5 3.672422372641922E-5 14-15 7.956915140724165E-4 0.0 0.0 3.672422372641922E-5 3.672422372641922E-5 16-17 8.813813694340613E-4 0.0 0.0 4.896563163522563E-5 3.672422372641922E-5 18-19 9.670712247957063E-4 6.120703954403204E-6 0.0 7.344844745283845E-5 3.672422372641922E-5 20-21 0.0010466403762029479 1.2241407908806408E-5 0.0 8.568985536164485E-5 3.672422372641922E-5 22-23 0.0010833645999293669 1.2241407908806408E-5 0.0 8.568985536164485E-5 3.672422372641922E-5 24-25 0.0011935372711086249 1.2241407908806408E-5 0.0 9.793126327045126E-5 3.672422372641922E-5 26-27 0.0013098306462422857 1.2241407908806408E-5 0.0 9.793126327045126E-5 4.284492768082243E-5 28-29 0.0013649169818319143 1.2241407908806408E-5 0.0 1.1017267117925767E-4 4.896563163522563E-5 30-31 0.0014873310609199784 1.2241407908806408E-5 0.0 1.1629337513366088E-4 6.120703954403204E-5 32-33 0.001560779508372817 1.2241407908806408E-5 0.0 1.2241407908806408E-4 6.120703954403204E-5 34-35 0.0016403486597800585 1.2241407908806408E-5 0.0 1.2241407908806408E-4 6.732774349843524E-5 36-37 0.0017505213309593163 1.2241407908806408E-5 0.0 1.2241407908806408E-4 7.344844745283845E-5 38-39 0.0018117283705033484 1.2241407908806408E-5 0.0 1.2241407908806408E-4 7.344844745283845E-5 40-41 0.0019647459693634284 1.2241407908806408E-5 0.0 1.2241407908806408E-4 8.568985536164485E-5 42-43 0.002019832304953057 1.2241407908806408E-5 0.0 1.2241407908806408E-4 8.568985536164485E-5 44-45 0.0021728499038131373 1.2241407908806408E-5 0.0 1.2241407908806408E-4 8.568985536164485E-5 46-47 0.002252419055220379 1.2241407908806408E-5 0.0 1.2241407908806408E-4 8.568985536164485E-5 48-49 0.002374833134308443 1.2241407908806408E-5 0.0 1.3465548699687047E-4 8.568985536164485E-5 50-51 0.0025829370687581524 1.2241407908806408E-5 0.0 1.468968949056769E-4 9.181055931604806E-5 52-53 0.0027175925557550223 1.2241407908806408E-5 0.0 1.591383028144833E-4 9.793126327045126E-5 54-55 0.0028155238190254736 2.4482815817612816E-5 0.0 1.591383028144833E-4 9.793126327045126E-5 56-57 0.0029501793060223444 2.4482815817612816E-5 0.0 1.713797107232897E-4 9.793126327045126E-5 58-59 0.0031154383127912307 2.4482815817612816E-5 0.0 1.713797107232897E-4 9.793126327045126E-5 60-61 0.0032317316879248914 2.4482815817612816E-5 0.0 1.775004146776929E-4 9.793126327045126E-5 62-63 0.003384749286784972 2.4482815817612816E-5 0.0 1.836211186320961E-4 9.793126327045126E-5 64-65 0.0035194047737818423 3.672422372641922E-5 0.0 1.836211186320961E-4 9.793126327045126E-5 66-67 0.00376423293195797 3.672422372641922E-5 0.0 1.836211186320961E-4 1.1017267117925767E-4 68-69 0.003984578274316485 4.284492768082243E-5 0.0 1.836211186320961E-4 1.1017267117925767E-4 70-71 0.004162078688994179 4.896563163522563E-5 0.0 1.836211186320961E-4 1.3465548699687047E-4 72-73 0.004382424031352694 5.5086335589628834E-5 0.0 1.8974182258649932E-4 1.468968949056769E-4 74-75 0.00456604514998479 6.120703954403204E-5 0.0 1.9586252654090253E-4 1.468968949056769E-4 76-77 0.004725183452799273 6.120703954403204E-5 0.0 1.9586252654090253E-4 1.468968949056769E-4 78-79 0.005000615130747418 6.120703954403204E-5 0.0 1.9586252654090253E-4 1.468968949056769E-4 80-81 0.005373978071966013 6.120703954403204E-5 0.0 2.2034534235851534E-4 1.468968949056769E-4 82-83 0.00582691016459185 6.120703954403204E-5 0.0 2.2034534235851534E-4 1.775004146776929E-4 84-85 0.006353290704670526 6.120703954403204E-5 0.0 2.2034534235851534E-4 1.836211186320961E-4 86-87 0.007112257995016523 6.732774349843524E-5 0.0 2.2034534235851534E-4 1.836211186320961E-4 88-89 0.008128294851447455 7.344844745283845E-5 0.0 2.2034534235851534E-4 1.836211186320961E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 197990 0.0 91.566 5 GGTATCA 198370 0.0 91.41169 8 GTATCAA 198515 0.0 91.365166 9 TGGTATC 198770 0.0 91.23968 7 AAGCAGT 199670 0.0 91.15916 1 GTGGTAT 200000 0.0 90.813995 6 AGCAGTG 202430 0.0 89.94483 2 CAGTGGT 201795 0.0 89.85122 4 GCAGTGG 202260 0.0 89.787926 3 ATCAACG 197605 0.0 46.30001 10-11 TCAACGC 197580 0.0 46.246723 12-13 CAACGCA 198000 0.0 46.201366 12-13 ACGCAGA 198790 0.0 46.05062 14-15 AACGCAG 198480 0.0 46.049606 14-15 TATCAAC 199190 0.0 45.820774 10-11 CGCAGAG 199570 0.0 45.820255 16-17 AGAGTAC 195600 0.0 45.64535 18-19 CAGAGTA 199140 0.0 45.482204 18-19 GCAGAGT 201835 0.0 45.243744 16-17 GAGTACT 113990 0.0 45.12282 20-21 >>END_MODULE