FastQCFastQC Report
Sat 18 Jun 2016
SRR578590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8168995
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA765480.9370552926033129No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91330.11180077843112891No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87000.10650024880661574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4550.022.927353
ATCAACG62950.018.97805410-11
CAACGCA65000.018.4524512-13
TCAACGC60750.017.24617810-11
CGTACGC2950.016.07259620-21
ACGTACG3150.015.80471920-21
AACGCAG67200.015.76721912-13
AGTGGTA78800.015.6451255
ACGCAGA79750.015.18822714-15
GGTATCA81350.015.0380468
GTATCAA82100.014.8994869
TGGTATC86100.014.3736357
TATCAAC82650.014.0545829
AAGCAGT97250.013.9464671
TCGCGTA3400.013.94337752-53
CGCGTAG3600.013.82718254-55
AGAGTAC89050.013.25664218-19
ACTCGCG4000.012.44446350-51
GTGGTAT101250.012.4102376
CGCAGAG85900.012.33477514-15
Produced by FastQC (version 0.11.5)