FastQCFastQC Report
Sat 18 Jun 2016
SRR578589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9062530
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108668911.99101134010039No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT231960.25595501476960625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126580.13967402038944976No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG93590.10327138227404489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2369250.091.2217251
AGTGGTA2355800.091.217015
GGTATCA2362000.091.04858
TGGTATC2363000.090.964727
GTATCAA2365200.090.904239
GTGGTAT2375900.090.549336
GCAGTGG2387850.090.2859953
AGCAGTG2394800.090.1968152
CAGTGGT2389200.089.8501364
TCAACGC2352750.046.28859712-13
AACGCAG2363850.046.1374114-15
CAACGCA2355150.046.08710512-13
ATCAACG2354700.046.06313710-11
CGCAGAG2373050.045.98945216-17
ACGCAGA2366050.045.92283214-15
TATCAAC2369600.045.87675510-11
CAGAGTA2360850.045.6285418-19
AGAGTAC2318850.045.55679718-19
GAGTACT1395850.045.4061520-21
GCAGAGT2392400.045.18763716-17
Produced by FastQC (version 0.11.5)