##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578589_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9062530 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.671089750875307 31.0 31.0 34.0 27.0 34.0 2 30.97241145684483 31.0 31.0 34.0 27.0 34.0 3 31.038571017144218 31.0 31.0 34.0 27.0 34.0 4 34.441946674935146 37.0 35.0 37.0 30.0 37.0 5 34.54975674563284 37.0 35.0 37.0 30.0 37.0 6 34.52410684433596 37.0 35.0 37.0 30.0 37.0 7 34.51762984508741 37.0 35.0 37.0 31.0 37.0 8 34.46687757171563 37.0 35.0 37.0 30.0 37.0 9 35.86607779505282 39.0 35.0 39.0 30.0 39.0 10-11 35.842890782154655 39.0 35.0 39.0 30.0 39.0 12-13 35.876469650307364 39.0 35.0 39.0 30.0 39.0 14-15 36.935155911208014 40.0 36.0 41.0 31.0 41.0 16-17 36.89432503947573 40.0 36.0 41.0 30.5 41.0 18-19 36.904135379413916 40.0 36.5 41.0 30.5 41.0 20-21 36.792043171167435 39.5 36.0 41.0 30.0 41.0 22-23 36.49138463541638 39.0 36.0 40.5 29.5 41.0 24-25 36.38953222775538 39.0 36.0 40.5 30.0 41.0 26-27 36.27988630106604 39.0 36.0 40.5 29.0 41.0 28-29 36.00555965056115 39.0 35.0 41.0 27.0 41.0 30-31 35.78663325804163 39.0 35.0 40.5 27.0 41.0 32-33 35.394710914060425 38.0 34.0 40.0 25.5 41.0 34-35 35.165222018575385 38.0 34.0 40.0 25.0 41.0 36-37 34.825013158577136 38.0 34.0 40.0 25.0 41.0 38-39 34.16396933306703 37.0 33.0 40.0 24.5 41.0 40-41 34.3474995944841 37.0 33.0 40.0 25.0 41.0 42-43 34.28171928810167 37.0 33.0 40.0 24.0 41.0 44-45 34.136217424935424 37.0 33.0 40.0 23.5 41.0 46-47 33.92565547369223 37.0 33.0 40.0 23.0 41.0 48-49 33.58948240723065 37.0 33.0 40.0 21.5 41.0 50-51 32.40260848791673 36.0 31.5 39.0 17.0 40.5 52-53 32.051134451416985 36.5 31.0 39.0 10.0 40.0 54-55 31.720024540608417 37.0 31.0 40.0 2.0 41.0 56-57 31.276849345602166 37.0 30.0 40.0 2.0 41.0 58-59 30.951604684343117 36.0 29.5 40.0 2.0 41.0 60-61 30.646782520995792 36.0 29.0 40.0 2.0 41.0 62-63 30.311942470811132 35.5 28.5 39.0 2.0 41.0 64-65 29.980129169227578 35.0 28.0 39.0 2.0 41.0 66-67 29.660552792652823 35.0 28.0 39.0 2.0 40.5 68-69 29.333465268528766 35.0 28.0 38.0 2.0 40.0 70-71 28.957130624671038 35.0 27.0 37.5 2.0 40.0 72-73 28.558717378039024 34.5 26.0 37.0 2.0 39.5 74-75 28.193538724837325 34.0 26.0 36.5 2.0 39.0 76-77 27.80463325362785 34.0 26.0 36.0 2.0 39.0 78-79 27.43576208851171 34.0 25.5 35.5 2.0 38.0 80-81 27.066866206236007 34.0 25.0 35.0 2.0 37.0 82-83 26.6888440645162 34.0 24.0 35.0 2.0 37.0 84-85 26.342572603897587 33.5 23.0 35.0 2.0 36.0 86-87 26.006428502857368 33.0 20.5 35.0 2.0 36.0 88-89 25.641221325612165 33.0 20.0 35.0 2.0 35.5 90-91 25.281688005446604 33.0 18.5 35.0 2.0 35.0 92-93 24.935675578453257 32.5 14.5 35.0 2.0 35.0 94-95 24.494916927171552 32.0 4.5 35.0 2.0 35.0 96-97 24.001123803176377 32.0 2.0 35.0 2.0 35.0 98-99 23.468341070319216 32.0 2.0 35.0 2.0 35.0 100-101 22.12950053682581 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5145397771355711 1101 2 0.5643660089208709 1101 3 0.6255726087283584 1101 4 0.7041764362516147 1101 5 0.5969584936389154 1101 6 0.5090474634549125 1101 7 0.4661781923932793 1101 8 0.5487530120916233 1101 9 0.7158583045167859 1101 10-11 0.772363326649824 1101 12-13 0.7307522043808063 1101 14-15 0.8089478468781905 1101 16-17 0.7521085198440005 1101 18-19 0.7684332343531608 1101 20-21 0.8028684509063808 1101 22-23 0.776757822411767 1101 24-25 0.8193025398586187 1101 26-27 1.0430995095669289 1101 28-29 1.0892670933083437 1101 30-31 1.1905214089514473 1101 32-33 1.1957816276770146 1101 34-35 1.2384700909213606 1101 36-37 1.1422669425870566 1101 38-39 1.0323792682150597 1101 40-41 1.1060652085424678 1101 42-43 1.3746302117429963 1101 44-45 1.337123171405615 1101 46-47 1.3474081195088417 1101 48-49 1.2765609333685006 1101 50-51 1.1756284555857874 1101 52-53 1.0262064805806759 1101 54-55 0.9934594915572923 1101 56-57 0.9894376532800493 1101 58-59 0.8910338928952974 1101 60-61 0.9610621087789752 1101 62-63 1.0349957662631653 1101 64-65 0.8831716163699532 1101 66-67 0.9431387274554694 1101 68-69 0.96281272076207 1101 70-71 1.0702590733040402 1101 72-73 1.1519995951253925 1101 74-75 1.0900494877897735 1101 76-77 1.153391525805059 1101 78-79 1.0150075622565353 1101 80-81 1.1154472161212183 1101 82-83 1.079309825553885 1101 84-85 1.1423039242761455 1101 86-87 1.2013050144670494 1101 88-89 1.2662073825425466 1101 90-91 1.2139512616809647 1101 92-93 1.3799702425243012 1101 94-95 1.5046740134458823 1101 96-97 1.6115246608503604 1101 98-99 1.7107385038387655 1101 100-101 2.0105886847697185 1102 1 0.573992970110929 1102 2 0.5628436381211124 1102 3 0.5053170704502925 1102 4 0.6421775305665278 1102 5 0.5382325520989326 1102 6 0.47945593745340176 1102 7 0.3660592460850154 1102 8 0.521930985533082 1102 9 0.6559788299742522 1102 10-11 0.738210454805106 1102 12-13 0.6353902232405488 1102 14-15 0.5972618988441738 1102 16-17 0.6201794759855801 1102 18-19 0.6208472145255328 1102 20-21 0.7775011490092041 1102 22-23 0.6692076705628196 1102 24-25 0.6430261293090993 1102 26-27 0.94087687924997 1102 28-29 0.8066976401308921 1102 30-31 0.9661808645397585 1102 32-33 0.9757555761510659 1102 34-35 0.9102684380046 1102 36-37 0.9254052886988617 1102 38-39 0.9764484429343838 1102 40-41 1.0479947691730302 1102 42-43 1.0843412252218698 1102 44-45 1.088924055773525 1102 46-47 1.166140643794364 1102 48-49 0.997656015671673 1102 50-51 1.1034841816761514 1102 52-53 1.011435669024408 1102 54-55 0.9395011611349595 1102 56-57 1.0003436524279294 1102 58-59 0.8102286953652502 1102 60-61 0.9612802398514617 1102 62-63 0.9638893615489579 1102 64-65 0.856996774833803 1102 66-67 0.9636544704861976 1102 68-69 0.998080866727797 1102 70-71 0.9717236011107069 1102 72-73 1.010921688287656 1102 74-75 1.1437051849142819 1102 76-77 1.116046976076607 1102 78-79 1.042911816134044 1102 80-81 1.0856211465415129 1102 82-83 1.0601567640781866 1102 84-85 1.0148879868015825 1102 86-87 1.1197550440428259 1102 88-89 1.1486290788104974 1102 90-91 1.2183263034053233 1102 92-93 1.356430384421504 1102 94-95 1.3540005573248273 1102 96-97 1.4671773385173985 1102 98-99 1.5244900544324231 1102 100-101 1.7227056828691971 1103 1 0.3537777391598418 1103 2 0.43009390823549154 1103 3 0.421966427801987 1103 4 0.48631693605521775 1103 5 0.43111657333275133 1103 6 0.30984049026027805 1103 7 0.23280224183380938 1103 8 0.3483203669677053 1103 9 0.405854470104245 1103 10-11 0.5199309588981791 1103 12-13 0.48518890288207217 1103 14-15 0.6034437265598172 1103 16-17 0.5389721233548812 1103 18-19 0.5137505354678282 1103 20-21 0.5780813821687474 1103 22-23 0.45190594919441907 1103 24-25 0.5503326213496749 1103 26-27 0.7454947531760183 1103 28-29 0.6526474674119882 1103 30-31 0.7902742517960348 1103 32-33 0.7834245853352613 1103 34-35 0.7784735764061566 1103 36-37 0.7994423762530474 1103 38-39 0.8144216636950787 1103 40-41 0.7038868880050444 1103 42-43 0.8470171759034386 1103 44-45 0.7435620008946131 1103 46-47 0.7916785235962607 1103 48-49 0.9155330339401004 1103 50-51 0.905306884531015 1103 52-53 0.8073378585785278 1103 54-55 0.736295151518096 1103 56-57 0.7090662550899012 1103 58-59 0.6584738833275985 1103 60-61 0.7577895015162461 1103 62-63 0.812047597331059 1103 64-65 0.7762034470509924 1103 66-67 0.8320561184316659 1103 68-69 0.7423065846274035 1103 70-71 0.675429051054838 1103 72-73 0.7251222532165293 1103 74-75 0.6855435094311311 1103 76-77 0.7303554738567932 1103 78-79 0.7697467153760158 1103 80-81 0.8376619793806981 1103 82-83 0.8871591448982521 1103 84-85 0.9317371278108872 1103 86-87 0.9086041278492232 1103 88-89 1.0295073641985546 1103 90-91 1.0928439141854618 1103 92-93 1.1091813737746143 1103 94-95 1.1892776144561061 1103 96-97 1.2437051735642903 1103 98-99 1.371594866087463 1103 100-101 1.6594787026168696 1104 1 0.3146783531240054 1104 2 0.24893734089298292 1104 3 0.32631042611708594 1104 4 0.4970542745829505 1104 5 0.34206457219632114 1104 6 0.29796870883898663 1104 7 0.350164975171289 1104 8 0.36688873055170035 1104 9 0.44233874960344366 1104 10-11 0.492062840545465 1104 12-13 0.40259887246511994 1104 14-15 0.43130236079726814 1104 16-17 0.5054564016648442 1104 18-19 0.4609398480413134 1104 20-21 0.522173004311604 1104 22-23 0.4246588411954946 1104 24-25 0.5015654552496969 1104 26-27 0.47239027104855325 1104 28-29 0.4854192182343695 1104 30-31 0.5279826659722815 1104 32-33 0.5415738009721665 1104 34-35 0.6385802064032902 1104 36-37 0.6697254440925988 1104 38-39 0.6163035024408572 1104 40-41 0.7022306701179915 1104 42-43 0.7613271122712675 1104 44-45 0.7994390663059932 1104 46-47 0.7708729493863018 1104 48-49 0.8420633747248729 1104 50-51 0.6500397874658788 1104 52-53 0.6787514745809133 1104 54-55 0.7050936966607573 1104 56-57 0.6441136108531573 1104 58-59 0.6693427805075416 1104 60-61 0.6051125549803764 1104 62-63 0.723715485139504 1104 64-65 0.7275568986802803 1104 66-67 0.5610634960624985 1104 68-69 0.5892148700813138 1104 70-71 0.5790998198937096 1104 72-73 0.616957452710583 1104 74-75 0.5895418280966886 1104 76-77 0.6889694408725298 1104 78-79 0.6471570988113662 1104 80-81 0.6101797961385529 1104 82-83 0.5413484064537393 1104 84-85 0.6292631472878689 1104 86-87 0.6568297063713366 1104 88-89 0.7388900024047089 1104 90-91 0.768312319475779 1104 92-93 0.8300081579150813 1104 94-95 0.9511897880999705 1104 96-97 0.9333128130399437 1104 98-99 0.9213165682524718 1104 100-101 1.2007340510335318 1105 1 0.07364719226763583 1105 2 0.1356061717943966 1105 3 0.237751926695001 1105 4 0.2826928170597185 1105 5 0.33800151478316565 1105 6 0.23650624259997244 1105 7 0.24296362054388965 1105 8 0.32189014288041307 1105 9 0.3236027440152327 1105 10-11 0.24812491377198853 1105 12-13 0.1941895019443578 1105 14-15 0.14434269996061744 1105 16-17 0.23648283849512097 1105 18-19 0.20471992391964022 1105 20-21 0.42968967737049013 1105 22-23 0.4143046681867091 1105 24-25 0.4453130850321969 1105 26-27 0.5702001944308108 1105 28-29 0.5664319557955864 1105 30-31 0.5426434570262231 1105 32-33 0.6273089847204076 1105 34-35 0.6379786583423197 1105 36-37 0.6653871699031058 1105 38-39 0.7107807424572243 1105 40-41 0.5774470456730114 1105 42-43 0.6988543793928415 1105 44-45 0.6836656285759162 1105 46-47 0.6531476867317849 1105 48-49 0.724838418554107 1105 50-51 0.6837394549950488 1105 52-53 0.5773248976968759 1105 54-55 0.623293778246488 1105 56-57 0.5469228351864288 1105 58-59 0.6473089713968783 1105 60-61 0.5298309561510841 1105 62-63 0.495402868574466 1105 64-65 0.5133205206822176 1105 66-67 0.6345867558066232 1105 68-69 0.4953320569455606 1105 70-71 0.404048642944403 1105 72-73 0.5153645736121355 1105 74-75 0.4743775881784309 1105 76-77 0.36993499625750914 1105 78-79 0.4530278905117875 1105 80-81 0.4039617278220362 1105 82-83 0.5112404392527594 1105 84-85 0.5577078135383005 1105 86-87 0.7042052058050992 1105 88-89 0.735317615228805 1105 90-91 0.7972751939047988 1105 92-93 0.8257952885249047 1105 94-95 0.8738910946042253 1105 96-97 0.982209352454138 1105 98-99 1.0967266271346432 1105 100-101 1.4715394768618246 1106 1 0.117583183341754 1106 2 0.21500817158332808 1106 3 0.2899006029541873 1106 4 0.49173150893013684 1106 5 0.34948544212664956 1106 6 0.3414460434545248 1106 7 0.3086028878337643 1106 8 0.20271036723644187 1106 9 0.18437601402075643 1106 10-11 0.34333124857744934 1106 12-13 0.32849930377538783 1106 14-15 0.30989404924044806 1106 16-17 0.2686638025655128 1106 18-19 0.12308281017073597 1106 20-21 0.2890354112810414 1106 22-23 0.34120629024662463 1106 24-25 0.35494519551235726 1106 26-27 0.2706652822931943 1106 28-29 0.3559608954886073 1106 30-31 0.4638202728756866 1106 32-33 0.4384146415442203 1106 34-35 0.36625079957524065 1106 36-37 0.47734896933055637 1106 38-39 0.6222371472315729 1106 40-41 0.33642290238802275 1106 42-43 0.31906361448614007 1106 44-45 0.4353296168539913 1106 46-47 0.4452452131555944 1106 48-49 0.42486644142656615 1106 50-51 0.5500368801456972 1106 52-53 0.5000589047210227 1106 54-55 0.3817522977735912 1106 56-57 0.272424237713242 1106 58-59 0.46917444006662024 1106 60-61 0.35057838813822784 1106 62-63 0.33822981665410623 1106 64-65 0.43274358500715593 1106 66-67 0.5197011071960205 1106 68-69 0.4900286532878262 1106 70-71 0.43065831740997496 1106 72-73 0.4932075163892655 1106 74-75 0.5106229458785414 1106 76-77 0.4226612403397283 1106 78-79 0.40822187783103914 1106 80-81 0.42549639004342055 1106 82-83 0.3474427649804781 1106 84-85 0.4165133964290568 1106 86-87 0.5493664044212245 1106 88-89 0.4615817331266392 1106 90-91 0.4563010515232939 1106 92-93 0.5326939476119179 1106 94-95 0.4609226319015711 1106 96-97 0.5497304312635087 1106 98-99 0.6078611367458535 1106 100-101 0.8330103168587542 1107 1 0.12651307197453932 1107 2 0.15799278386532833 1107 3 0.260870956762286 1107 4 0.46775947050414146 1107 5 0.2643987680638986 1107 6 0.3321123586309156 1107 7 0.3211409946159023 1107 8 0.21302491349214847 1107 9 0.22621975372666725 1107 10-11 0.28460900381751486 1107 12-13 0.25552902005082245 1107 14-15 0.4086747463174092 1107 16-17 0.29105722130377387 1107 18-19 0.32167229398198316 1107 20-21 0.2792385441109886 1107 22-23 0.28930578550024677 1107 24-25 0.527385304468865 1107 26-27 0.2590207177855248 1107 28-29 0.18100037510134115 1107 30-31 0.3431783845846681 1107 32-33 0.5396556450323047 1107 34-35 0.33616051516057155 1107 36-37 0.37040617603880577 1107 38-39 0.4868396563340198 1107 40-41 0.2773897717595162 1107 42-43 0.3595309806211944 1107 44-45 0.5281643899179187 1107 46-47 0.47556661235343967 1107 48-49 0.5380084841307209 1107 50-51 0.33380960542002214 1107 52-53 0.45008388296368906 1107 54-55 0.38749589579596133 1107 56-57 0.5402238785801856 1107 58-59 0.7727099183053454 1107 60-61 0.6929325578137089 1107 62-63 0.5557524215900216 1107 64-65 0.6657482616968657 1107 66-67 0.7945530323327006 1107 68-69 0.7305195742006738 1107 70-71 0.5231749931440284 1107 72-73 0.5838248278604148 1107 74-75 0.6779540539968174 1107 76-77 0.7155206464774899 1107 78-79 0.6351231308235832 1107 80-81 0.6011846176215272 1107 82-83 0.6323647484367712 1107 84-85 0.6124964675692901 1107 86-87 0.8330895737201693 1107 88-89 0.8167967224775836 1107 90-91 0.769956161172825 1107 92-93 0.9406603313850539 1107 94-95 0.808136847252765 1107 96-97 0.7646204762759226 1107 98-99 0.8695379617028394 1107 100-101 1.0026365809369189 1108 1 0.158197444264772 1108 2 0.14924625685781834 1108 3 0.16158426799991688 1108 4 0.3867739844028435 1108 5 0.21137890424549255 1108 6 0.3236440404301675 1108 7 0.22633859450816374 1108 8 0.21033738737871488 1108 9 0.3175019978102753 1108 10-11 0.2716951740895439 1108 12-13 0.11948466798533275 1108 14-15 0.3580974358182516 1108 16-17 0.26416250754655834 1108 18-19 0.2459929431589174 1108 20-21 0.3091963573295544 1108 22-23 0.2884549894875974 1108 24-25 0.4760836773733388 1108 26-27 0.41640982860015896 1108 28-29 0.36348774675113305 1108 30-31 0.3291149496883605 1108 32-33 0.5755746835576971 1108 34-35 0.40243211528571265 1108 36-37 0.46971810943711745 1108 38-39 0.4198466970090706 1108 40-41 0.5185618789788649 1108 42-43 0.5077105557045911 1108 44-45 0.46508512855130135 1108 46-47 0.4040795259589629 1108 48-49 0.5399062358922322 1108 50-51 0.29543920010658553 1108 52-53 0.3120967135378976 1108 54-55 0.22617815056890933 1108 56-57 0.07857223153771287 1108 58-59 0.2742243936084243 1108 60-61 0.26741751012094284 1108 62-63 0.1719191718645341 1108 64-65 0.12622388985590405 1108 66-67 0.3169102776798809 1108 68-69 0.37724942708610243 1108 70-71 0.30754238605547 1108 72-73 0.2654506415635218 1108 74-75 0.3411044935994063 1108 76-77 0.32365917545904566 1108 78-79 0.2569496548100645 1108 80-81 0.3005523123582421 1108 82-83 0.3542293267501364 1108 84-85 0.3189683466463187 1108 86-87 0.3526441199742898 1108 88-89 0.32491771206901987 1108 90-91 0.25996695641761036 1108 92-93 0.35398281246096985 1108 94-95 0.48787408566489887 1108 96-97 0.5007872805736326 1108 98-99 0.5294490751741705 1108 100-101 0.7280744950298264 1109 1 -0.37333740350296196 1109 2 0.01506811671177033 1109 3 0.05179945737237546 1109 4 0.13742903141423568 1109 5 0.020420609305027426 1109 6 -0.02779523610168866 1109 7 0.03637550592146255 1109 8 0.06584361728354082 1109 9 0.09901486315042263 1109 10-11 0.03022356510971491 1109 12-13 0.022088969183293727 1109 14-15 -0.017329220736534978 1109 16-17 -0.061687851003227934 1109 18-19 0.017289756799492295 1109 20-21 0.1090375234220744 1109 22-23 0.03904742036321096 1109 24-25 0.0661518479348544 1109 26-27 0.19309415446619482 1109 28-29 -0.05452014479894984 1109 30-31 -0.04794745717177307 1109 32-33 0.20349842237339288 1109 34-35 0.16688675823106536 1109 36-37 0.2150628629340261 1109 38-39 0.2534160511857735 1109 40-41 0.22003075453260124 1109 42-43 0.17644257890270154 1109 44-45 0.13966284334130563 1109 46-47 0.24724219066453657 1109 48-49 0.36330692341164195 1109 50-51 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0.1302735607568195 2311 8 0.048074101321020635 2311 9 0.20312735079738786 2311 10-11 0.12636168532603875 2311 12-13 -0.04449952199482965 2311 14-15 0.0790890835311231 2311 16-17 0.12198391600161074 2311 18-19 -0.1393000679889127 2311 20-21 0.06558300773154713 2311 22-23 -0.021861373155765307 2311 24-25 0.036386044118764005 2311 26-27 -0.04693224225782444 2311 28-29 0.12033853281780438 2311 30-31 0.09097553837631978 2311 32-33 -0.1472114600934944 2311 34-35 -0.07192521315024436 2311 36-37 0.08319934282874186 2311 38-39 0.07936752647548673 2311 40-41 -0.09933524506585911 2311 42-43 -0.06371923649825817 2311 44-45 -0.024826638772943 2311 46-47 0.28436267110785707 2311 48-49 0.14397665919452152 2311 50-51 0.23992293400004883 2311 52-53 0.14859603165775326 2311 54-55 0.12456479429512157 2311 56-57 0.20929996034593046 2311 58-59 -0.026141403180265144 2311 60-61 -0.08453055409109922 2311 62-63 -0.13126405727664547 2311 64-65 -0.1495341712848557 2311 66-67 -0.0064278367226151545 2311 68-69 0.09550792415947384 2311 70-71 0.16137013633180786 2311 72-73 0.09574252015272933 2311 74-75 0.060597935892719335 2311 76-77 0.056621414336259335 2311 78-79 0.03589565405147965 2311 80-81 0.06134847339646399 2311 82-83 0.16009551143117662 2311 84-85 0.07603983481990184 2311 86-87 0.14088555029126226 2311 88-89 0.23719162444620423 2311 90-91 0.22470591295698128 2311 92-93 0.04113721677041582 2311 94-95 0.06384914343257364 2311 96-97 0.2430945541013081 2311 98-99 0.2962609794744253 2311 100-101 0.3033586835861115 2312 1 0.05010081495576202 2312 2 -0.2623406858857926 2312 3 0.0024248834802591546 2312 4 0.10542084580492883 2312 5 0.158723805976706 2312 6 0.03469328443872399 2312 7 0.02349996124005571 2312 8 0.0458204445132111 2312 9 0.0867903948396389 2312 10-11 -0.021100111666669363 2312 12-13 -0.045652538634882944 2312 14-15 0.019170154478239 2312 16-17 -0.15019996751310316 2312 18-19 -0.1011129694398889 2312 20-21 -0.09880373230786432 2312 22-23 -0.015468996037334648 2312 24-25 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42-43 -0.10269793300845009 2313 44-45 -0.17576578836629864 2313 46-47 -0.3426611413034024 2313 48-49 -0.27580364016888126 2313 50-51 -0.29486596699670997 2313 52-53 -0.3254970983855259 2313 54-55 -0.2021632706565093 2313 56-57 -0.12202500024459795 2313 58-59 -0.08585720333521962 2313 60-61 -0.20008545967202807 2313 62-63 -0.2530039802469659 2313 64-65 -0.19280319911354482 2313 66-67 -0.17041857477128985 2313 68-69 -0.2633779643296137 2313 70-71 -0.11197228341397647 2313 72-73 -0.17936181958345188 2313 74-75 -0.2967314272193704 2313 76-77 -0.35235304479940055 2313 78-79 -0.2118407809202978 2313 80-81 -0.4135637722355838 2313 82-83 -0.34304678714165604 2313 84-85 -0.36246763147433825 2313 86-87 -0.4185238777922464 2313 88-89 -0.4465433034185651 2313 90-91 -0.31409211111462554 2313 92-93 -0.33654939205571566 2313 94-95 -0.2813851372599032 2313 96-97 -0.22117299228820642 2313 98-99 -0.26865050942421576 2313 100-101 -0.39459634258998477 2314 1 -0.6411292496170446 2314 2 -0.8825406106173297 2314 3 -0.5354106204901647 2314 4 -0.7268060576410917 2314 5 -0.6223288375988787 2314 6 -0.5227811340702786 2314 7 -0.44034101468320586 2314 8 -0.49942187261576976 2314 9 -0.390706430778593 2314 10-11 -0.523662955754169 2314 12-13 -0.4653634215180986 2314 14-15 -0.6224574678088501 2314 16-17 -0.7515689318829857 2314 18-19 -2.4097011060102815 2314 20-21 -1.0700119893424827 2314 22-23 -0.733461206073514 2314 24-25 -0.8798325423105169 2314 26-27 -0.9583834369314985 2314 28-29 -0.6214761520479044 2314 30-31 -0.6919443103563765 2314 32-33 -0.6954197566513116 2314 34-35 -0.8653592661587695 2314 36-37 -0.7632118094551643 2314 38-39 -0.5527176215257938 2314 40-41 -0.45020752915441875 2314 42-43 -0.8421030709345629 2314 44-45 -0.7213370507078523 2314 46-47 -0.6015417478930303 2314 48-49 -0.5886918454446501 2314 50-51 -0.661256264766422 2314 52-53 -0.6384838556886123 2314 54-55 -0.5915652517337087 2314 56-57 -0.5668262436030389 2314 58-59 -0.6402455768361826 2314 60-61 -0.5148498121484266 2314 62-63 -0.6282740614029478 2314 64-65 -0.6155531878037692 2314 66-67 -0.6586362307281561 2314 68-69 -0.6299464893346034 2314 70-71 -0.7180890947345304 2314 72-73 -0.7263493476817509 2314 74-75 -0.6702768503693797 2314 76-77 -0.6666730701728518 2314 78-79 -0.6850976428641502 2314 80-81 -0.5798042469940192 2314 82-83 -0.6979035262724693 2314 84-85 -0.7074424446652721 2314 86-87 -0.8290257722724093 2314 88-89 -0.7961562025454967 2314 90-91 -0.6405052212584934 2314 92-93 -0.5279582094289168 2314 94-95 -0.6111998621183936 2314 96-97 -0.7224053400765449 2314 98-99 -0.784398513279676 2314 100-101 -0.7604435239095473 2315 1 -0.8667403991602356 2315 2 -1.6555479040979222 2315 3 -1.1868434445322116 2315 4 -0.45785907005333115 2315 5 -0.8107297683177705 2315 6 -1.2884704266284999 2315 7 -1.237408305937386 2315 8 -1.3540423691051942 2315 9 -1.4429491313344016 2315 10-11 -0.7945729958693022 2315 12-13 -1.6836021523053688 2315 14-15 -8.758208866272632 2315 16-17 -4.231030483571473 2315 18-19 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-1.3627335786328594 2315 84-85 -1.4637494218763756 2315 86-87 -1.5878001981766872 2315 88-89 -1.4326176866465445 2315 90-91 -1.487460779530796 2315 92-93 -1.5156552417436657 2315 94-95 -1.479644366861443 2315 96-97 -1.5926552049016536 2315 98-99 -1.6528025748876196 2315 100-101 -1.988082048356322 2316 1 -0.867423138163705 2316 2 -1.0943896032664853 2316 3 -1.4836412359304632 2316 4 -1.3177510004202588 2316 5 -0.8008955794442443 2316 6 -0.7794748879203723 2316 7 -1.0281976455148012 2316 8 -1.0451391570281672 2316 9 -1.3193650304384263 2316 10-11 -1.350122084521459 2316 12-13 -1.2254320754295946 2316 14-15 -1.26270487192577 2316 16-17 -2.017285389564975 2316 18-19 -1.3843577356320296 2316 20-21 -1.7278677984847093 2316 22-23 -1.2485712922853622 2316 24-25 -1.1574133375646838 2316 26-27 -1.285781580215982 2316 28-29 -1.689961380291031 2316 30-31 -1.5571715251116984 2316 32-33 -1.6563868547973115 2316 34-35 -2.0085505110837047 2316 36-37 -1.7530863984844487 2316 38-39 -1.683479604831895 2316 40-41 -2.0437707775596863 2316 42-43 -2.2251924515824157 2316 44-45 -2.520470425512137 2316 46-47 -2.3923305898554474 2316 48-49 -2.8926725675029274 2316 50-51 -2.4002710894542076 2316 52-53 -3.079775773188981 2316 54-55 -4.1523520772804865 2316 56-57 -2.906318711786522 2316 58-59 -2.3617407288688774 2316 60-61 -2.104718477956773 2316 62-63 -2.7475168670839984 2316 64-65 -2.779112126120605 2316 66-67 -2.4714232145635577 2316 68-69 -2.4037896716820946 2316 70-71 -2.7176243058885383 2316 72-73 -5.806365821797531 2316 74-75 -3.497810043091331 2316 76-77 -2.8800704230223992 2316 78-79 -2.852151084936054 2316 80-81 -2.732883122116391 2316 82-83 -2.767282893702717 2316 84-85 -2.8817950744241365 2316 86-87 -2.6662843291842258 2316 88-89 -2.3918723980934544 2316 90-91 -2.537831227273461 2316 92-93 -2.8902682857603317 2316 94-95 -3.153693719612253 2316 96-97 -3.147915689833919 2316 98-99 -3.436573671240634 2316 100-101 -3.509698589397683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81963.0 3 15081.0 4 13339.0 5 13197.0 6 15262.0 7 19198.0 8 24104.0 9 28351.0 10 32242.0 11 38266.0 12 40112.0 13 45585.0 14 51171.0 15 61770.0 16 78101.0 17 103747.0 18 146639.0 19 212643.0 20 274618.0 21 205268.0 22 126511.0 23 113389.0 24 118215.0 25 127336.0 26 140735.0 27 159399.0 28 185706.0 29 216093.0 30 254108.0 31 304695.0 32 369217.0 33 456826.0 34 587920.0 35 809991.0 36 1159151.0 37 1282298.0 38 986910.0 39 163202.0 40 171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.6903455975014 46.62533704214542 25.34698316973537 17.337334190617813 2 16.83246103364862 48.52785071483222 18.673511804317442 15.966176447201722 3 42.73228421592522 21.365805257691562 19.687782871395648 16.21412765498757 4 16.79913221302043 19.736780422739894 20.60856978339379 42.855517580845884 5 16.626177957511644 45.92516175882555 21.673921284825507 15.774738998837295 6 42.85056651161712 20.852520323676746 19.5096472921921 16.78726587251404 7 17.512937389353457 20.268900649951114 46.56252913204509 15.655632828650337 8 42.40323779113482 21.042472705489647 20.426728254732858 16.127561248642674 9 42.069244645530205 20.649366640586134 20.981482084771493 16.299906629112172 10-11 16.36140888478849 33.50702725755767 33.51936783973609 16.612196017917753 12-13 16.17588762153118 21.013946161443233 33.48609756593116 29.32406865109442 14-15 16.334483258792197 46.5909419061722 20.45928478166536 16.61529005337024 16-17 29.21724196110093 20.8008445680345 20.61334865351422 29.368564817350347 18-19 16.511452857452397 33.53964164018002 20.57207840690939 29.37682709545819 20-21 29.399873907990592 33.60581138320152 20.60944140003311 16.38487330877478 22-23 29.34351153465087 20.726118622410258 33.47262124561534 16.45774859732353 24-25 16.44517108876778 33.383421377933644 21.011825234803233 29.159582298495344 26-27 16.673336313239425 25.52300608569832 41.070443500770715 16.73321410029154 28-29 26.491160468951925 20.61183034949173 36.30396640606729 16.593042775489053 30-31 24.691642838342382 21.56439017656922 36.64040248044535 17.10356450464304 32-33 19.77335967526459 23.788149276953728 37.721818498427126 18.716672549354556 34-35 19.30326499244801 22.95984478482099 38.36880319748215 19.368087025248855 36-37 18.877643561730974 23.03262064844312 38.62596556675599 19.46377022306992 38-39 19.289732191181976 22.95248183209077 38.46415113024375 19.293634846483506 40-41 19.22057868427935 22.952707108909095 38.37338428991555 19.45332991689601 42-43 19.648515725908837 23.149012507865717 38.252287956901405 18.950183809324045 44-45 19.156910911883624 23.298719682419506 38.199756745069294 19.34461266062758 46-47 19.435409061720105 23.101939466290474 38.08155038078503 19.381101091204382 48-49 19.367296446020354 23.16688021387175 38.24334600928348 19.222477330824418 50-51 19.6976517931333 23.273541432610084 37.52597138012143 19.502835394135186 52-53 19.917361707863186 24.202010503400782 36.46623137717258 19.414396411563445 54-55 20.54504112536371 24.682001847419286 35.19974565557028 19.573211371646725 56-57 20.517281024977883 25.403499158211893 34.04235577913944 20.036864037670775 58-59 20.70517251334255 26.187029375959714 33.1130597270738 19.994738383623943 60-61 20.96428843480939 26.285513676663946 32.72804651732753 20.022151371199133 62-63 21.011543246213964 26.16767780657566 32.71823487562714 20.10254407158323 64-65 20.4619732795412 25.929557020816112 32.9177448019326 20.69072489771009 66-67 20.45738128589755 25.5835537778447 33.518811038052164 20.440253898205583 68-69 20.505817227081742 25.495165157717803 33.58849690213194 20.410520713068518 70-71 20.305765105539223 25.368539938638317 34.139876592141334 20.185818363681125 72-73 20.04754661686812 25.283756889220204 33.96684850995689 20.70184798395478 74-75 20.31932564189806 25.523160525905 33.363729317942806 20.793784514254135 76-77 20.766555157732107 25.842475713619002 33.0009518875884 20.390017241060495 78-79 20.715478755817752 25.94974543190469 32.72179769674383 20.612978115533732 80-81 20.4795277912718 26.28253471187095 32.65075434646646 20.587183150390796 82-83 21.024507380971503 26.40994922227199 32.156175390285306 20.4093680064712 84-85 20.815195519674614 26.734919766582653 31.676953186212103 20.772931527530627 86-87 20.77585126706826 27.158581007196307 31.415851973617027 20.649715752118407 88-89 20.754406220653298 26.714222649606285 31.60476632091904 20.926604808821374 90-91 20.697478649747676 26.79808063134518 31.60267878802665 20.901761930880493 92-93 20.650712750076018 27.08986230602538 31.555708604668787 20.703716339229814 94-95 20.751475910548923 26.7356110278228 31.202998533817816 21.309914527810463 96-97 20.669691891331652 26.47654926591833 31.562520592289715 21.2912382504603 98-99 21.058459019652506 26.738237530544133 31.16657365835814 21.036729791445218 100-101 21.330841580724325 26.45989954294507 30.899514855457983 21.30974402087262 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15026.0 1 9124.5 2 2368.5 3 1337.5 4 1114.0 5 975.5 6 1038.5 7 1249.0 8 1167.0 9 1230.0 10 1670.0 11 9898.0 12 185022.0 13 207490.5 14 55348.5 15 49156.0 16 52056.5 17 54944.0 18 56837.5 19 57423.0 20 58281.0 21 59492.0 22 61220.0 23 64718.0 24 70212.0 25 77362.5 26 85290.5 27 94227.5 28 104284.5 29 115299.5 30 124741.5 31 133663.0 32 144135.5 33 156064.0 34 175005.5 35 189398.0 36 198305.0 37 210278.0 38 220223.0 39 231086.0 40 245689.5 41 256638.5 42 262602.0 43 273644.0 44 288909.0 45 297668.0 46 297201.0 47 295238.5 48 296006.0 49 294934.0 50 290101.5 51 284994.5 52 279157.0 53 273429.5 54 259429.5 55 238275.5 56 220358.0 57 206264.5 58 205159.0 59 177708.5 60 131136.5 61 101539.0 62 74663.5 63 54137.0 64 38626.0 65 26453.5 66 17035.0 67 10611.5 68 6565.5 69 4184.0 70 2901.0 71 2333.0 72 1955.0 73 1654.5 74 1577.0 75 1477.5 76 1322.0 77 1234.5 78 1187.5 79 1111.0 80 986.0 81 898.5 82 854.5 83 778.5 84 681.5 85 578.5 86 505.5 87 438.5 88 359.0 89 305.5 90 238.0 91 158.5 92 115.0 93 91.0 94 69.5 95 43.5 96 25.0 97 19.5 98 13.5 99 5.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10082173521080759 2 0.08317765568776048 3 0.088959705512699 4 0.1976710697785276 5 0.07634733347089609 6 0.0930755539567869 7 0.09849346705610906 8 0.08785626088961912 9 0.07994456294213648 10-11 0.07769905313416894 12-13 0.07081907590926595 14-15 0.07741215753216817 16-17 0.06693495083602481 18-19 0.058399806676502033 20-21 0.05361085701233541 22-23 0.043641234842808796 24-25 0.045765365742237545 26-27 0.04500950617542783 28-29 0.044021923237771356 30-31 0.03430057610843771 32-33 0.038206770074140445 34-35 0.05134327831190627 36-37 0.046885362034663605 38-39 0.05069776320740456 40-41 0.04812122001251306 42-43 0.04667570755627843 44-45 0.05012948922651843 46-47 0.04421502604681033 48-49 0.04019848761879961 50-51 0.04414881936942554 52-53 0.04032538375045379 54-55 0.04355847649607781 56-57 0.042339170187574555 58-59 0.04940121577528571 60-61 0.048551563415514205 62-63 0.05259017073598653 64-65 0.04912535461951575 66-67 0.043464683703116014 68-69 0.04371295874330899 70-71 0.052523964058601734 72-73 0.04006607426403002 74-75 0.04762466993212712 76-77 0.046482604747239455 78-79 0.04468950723473467 80-81 0.044066061022694544 82-83 0.04339295980261583 84-85 0.04404399213023295 86-87 0.04871708010897619 88-89 0.04196399901572739 90-91 0.0444743355332341 92-93 0.04093227829314772 94-95 0.04380123431315538 96-97 0.02987024594677204 98-99 0.036452293123443454 100-101 0.02472267678010445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9062530.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.49034936924546 #Duplication Level Percentage of deduplicated Percentage of total 1 92.69063708943645 49.58054561171897 2 4.3437864525240055 4.647013098618089 3 1.1035191869879974 1.7708288054296109 4 0.47534611570261714 1.0170571920098708 5 0.2888442979797027 0.7725191206124369 6 0.18547245663942188 0.5952591902408945 7 0.11833003054322133 0.4430660272241277 8 0.09086577656387279 0.3888353707287482 9 0.06550648457670012 0.31535682713629015 >10 0.6095192937930544 6.12172274607253 >50 0.013243706138167185 0.45235625727931994 >100 0.008525917723757501 1.0532530074990756 >500 0.0029518258188916424 1.1083443698951871 >1k 0.003088063932986687 3.4995912138313265 >5k 2.2706352448431522E-4 0.9660938463720746 >10k+ 1.3623811469058912E-4 27.26815731533147 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1086689 11.99101134010039 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 23196 0.25595501476960625 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12658 0.13967402038944976 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9359 0.10327138227404489 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 1.1034446230798683E-5 0.0 0.0 0.0 0.0 5 1.1034446230798683E-5 0.0 0.0 1.1034446230798683E-5 0.0 6 1.1034446230798683E-5 0.0 0.0 1.1034446230798683E-5 1.1034446230798683E-5 7 1.1034446230798683E-5 0.0 0.0 1.1034446230798683E-5 1.1034446230798683E-5 8 1.1034446230798683E-5 0.0 0.0 1.1034446230798683E-5 1.1034446230798683E-5 9 1.1034446230798683E-5 0.0 0.0 1.1034446230798683E-5 1.1034446230798683E-5 10-11 1.1034446230798683E-5 0.0 0.0 1.6551669346198027E-5 1.1034446230798683E-5 12-13 1.1034446230798683E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 14-15 1.1034446230798683E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 16-17 1.1034446230798683E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 18-19 1.1034446230798683E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 20-21 1.6551669346198027E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 22-23 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 24-25 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 26-27 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 28-29 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 30-31 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 32-33 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 34-35 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 36-37 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 38-39 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 40-41 2.2068892461597367E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 42-43 3.3103338692396054E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 44-45 4.4137784923194734E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 46-47 8.275834673099013E-5 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 48-49 1.3793057788498355E-4 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 50-51 1.5999947034658093E-4 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 52-53 2.09654478385175E-4 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 54-55 2.4275781707757107E-4 0.0 0.0 2.2068892461597367E-5 1.1034446230798683E-5 56-57 2.979300482315645E-4 0.0 0.0 2.2068892461597367E-5 2.2068892461597367E-5 58-59 3.917228411933533E-4 5.517223115399342E-6 0.0 2.2068892461597367E-5 2.2068892461597367E-5 60-61 4.4137784923194736E-4 2.2068892461597367E-5 0.0 2.2068892461597367E-5 2.2068892461597367E-5 62-63 5.075845266167395E-4 2.2068892461597367E-5 0.0 2.2068892461597367E-5 2.2068892461597367E-5 64-65 6.565495507325216E-4 2.2068892461597367E-5 0.0 2.2068892461597367E-5 2.2068892461597367E-5 66-67 7.282734512327132E-4 2.2068892461597367E-5 0.0 3.3103338692396054E-5 2.2068892461597367E-5 68-69 8.05514574848304E-4 2.2068892461597367E-5 0.0 3.8620561807795394E-5 4.4137784923194734E-5 70-71 8.441351366560994E-4 2.2068892461597367E-5 0.0 4.4137784923194734E-5 4.4137784923194734E-5 72-73 9.931001607718817E-4 2.2068892461597367E-5 0.0 4.4137784923194734E-5 5.517223115399342E-5 74-75 0.0011972374160416572 2.2068892461597367E-5 0.0 4.4137784923194734E-5 5.517223115399342E-5 76-77 0.0013572368863882381 2.2068892461597367E-5 0.0 4.4137784923194734E-5 5.517223115399342E-5 78-79 0.0015172363567348191 2.2068892461597367E-5 0.0 4.4137784923194734E-5 5.517223115399342E-5 80-81 0.0018482697436587796 2.2068892461597367E-5 0.0 4.4137784923194734E-5 6.620667738479211E-5 82-83 0.002405509278314113 2.2068892461597367E-5 0.0 4.4137784923194734E-5 7.724112361559079E-5 84-85 0.003062058829046635 2.2068892461597367E-5 0.0 4.4137784923194734E-5 7.724112361559079E-5 86-87 0.0038344700652025426 2.2068892461597367E-5 0.0 4.965500803859408E-5 7.724112361559079E-5 88-89 0.0051144658279751905 2.2068892461597367E-5 0.0 5.517223115399342E-5 7.724112361559079E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 236925 0.0 91.221725 1 AGTGGTA 235580 0.0 91.21701 5 GGTATCA 236200 0.0 91.0485 8 TGGTATC 236300 0.0 90.96472 7 GTATCAA 236520 0.0 90.90423 9 GTGGTAT 237590 0.0 90.54933 6 GCAGTGG 238785 0.0 90.285995 3 AGCAGTG 239480 0.0 90.196815 2 CAGTGGT 238920 0.0 89.850136 4 TCAACGC 235275 0.0 46.288597 12-13 AACGCAG 236385 0.0 46.13741 14-15 CAACGCA 235515 0.0 46.087105 12-13 ATCAACG 235470 0.0 46.063137 10-11 CGCAGAG 237305 0.0 45.989452 16-17 ACGCAGA 236605 0.0 45.922832 14-15 TATCAAC 236960 0.0 45.876755 10-11 CAGAGTA 236085 0.0 45.62854 18-19 AGAGTAC 231885 0.0 45.556797 18-19 GAGTACT 139585 0.0 45.40615 20-21 GCAGAGT 239240 0.0 45.187637 16-17 >>END_MODULE