FastQCFastQC Report
Sat 18 Jun 2016
SRR578589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9062530
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1431201.5792499445519077No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296120.32675202178641066No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99450.10973756776529292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4050.030.493183
CGCGTAG2850.023.33309454-55
ATCAACG62300.020.73700710-11
CAACGCA62350.020.644212-13
CGTACGC2900.020.4729720-21
TCGCGTA3550.018.06399352-53
CTCGCGT3500.017.64345452-53
ACTCGCG3700.017.33233850-51
GGTATCA84050.017.1791278
TCAACGC62700.017.15796910-11
AGTGGTA85550.016.711545
CGCTAGT3750.016.46631690-91
GTATCAA89500.016.1860929
AACGCAG68050.015.77413312-13
ACGCAGA82550.015.67895214-15
TGGTATC93850.015.6382947
AAGCAGT97850.015.4593381
ACGTACG4050.014.65965720-21
GTGGTAT104400.013.9216586
CGTAGCG4300.013.80746556-57
Produced by FastQC (version 0.11.5)