##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578589_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9062530 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48141490290239 31.0 31.0 34.0 30.0 34.0 2 31.794617728162002 31.0 31.0 34.0 30.0 34.0 3 31.86618184988077 31.0 31.0 34.0 30.0 34.0 4 35.54163594492928 37.0 35.0 37.0 33.0 37.0 5 35.36435851798559 37.0 35.0 37.0 33.0 37.0 6 35.36081888832368 37.0 35.0 37.0 32.0 37.0 7 35.33821559763113 37.0 35.0 37.0 32.0 37.0 8 35.32697756586737 37.0 35.0 37.0 32.0 37.0 9 36.89439748061524 39.0 37.0 39.0 33.0 39.0 10-11 36.859944132598734 39.0 37.0 39.0 32.5 39.0 12-13 36.83904902935494 39.0 37.0 39.0 32.0 39.0 14-15 38.02141774978952 40.0 37.0 41.0 33.0 41.0 16-17 37.96595062306001 40.0 37.0 41.0 33.0 41.0 18-19 37.889052008655426 40.0 37.0 41.0 33.0 41.0 20-21 37.833755695153556 40.0 37.0 41.0 32.0 41.0 22-23 37.73992588162467 40.0 37.0 41.0 32.0 41.0 24-25 37.649231561164484 39.0 36.0 41.0 32.0 41.0 26-27 37.465513327955875 39.0 36.0 41.0 32.0 41.0 28-29 37.29488487210525 39.0 36.0 41.0 31.5 41.0 30-31 37.165023398543234 39.0 36.0 41.0 31.0 41.0 32-33 37.03443696186385 39.0 36.0 40.5 31.0 41.0 34-35 36.88308562840619 39.0 35.5 40.0 30.0 41.0 36-37 36.735608378675714 39.0 35.0 40.0 30.0 41.0 38-39 36.570894496349254 38.0 35.0 40.0 30.0 41.0 40-41 36.403181948087344 38.0 35.0 40.0 30.0 41.0 42-43 36.3466153491354 38.0 35.0 40.0 30.0 41.0 44-45 36.37903515905603 38.0 35.0 40.0 30.0 41.0 46-47 36.251821897417166 38.0 35.0 40.0 30.0 41.0 48-49 36.1126082892967 38.0 35.0 40.0 29.0 41.0 50-51 35.91639200090924 38.0 34.5 40.0 28.5 41.0 52-53 35.97603809311528 38.0 35.0 40.0 29.0 41.0 54-55 35.96713941912468 38.0 35.0 40.0 28.5 41.0 56-57 35.806679039959036 38.0 34.0 40.0 28.0 41.0 58-59 35.59993920020126 38.0 34.0 40.0 28.0 41.0 60-61 35.33944323494653 38.0 34.0 40.0 28.0 41.0 62-63 35.0569364735896 37.0 34.0 40.0 27.5 41.0 64-65 34.7351934283252 37.0 33.0 40.0 26.5 41.0 66-67 34.39045702469399 36.0 33.0 39.0 26.0 41.0 68-69 34.024209685374835 36.0 33.0 39.0 26.0 41.0 70-71 33.63889151263499 35.5 32.0 39.0 26.0 40.0 72-73 33.24432388085888 35.0 32.0 38.0 26.0 40.0 74-75 32.83172712255849 35.0 32.0 37.0 25.0 39.5 76-77 31.47328240568583 34.0 30.0 36.0 23.0 39.0 78-79 32.11727221868507 34.5 31.0 36.0 25.0 39.0 80-81 31.99252394198971 35.0 31.0 36.0 24.5 38.5 82-83 31.677959024687368 34.5 31.0 36.0 24.0 37.0 84-85 31.35168915302901 34.0 31.0 35.0 23.0 37.0 86-87 31.002316792330618 34.0 31.0 35.0 21.5 36.5 88-89 30.68007394182419 34.0 31.0 35.0 20.0 36.0 90-91 30.35802292516549 34.0 30.0 35.0 19.5 36.0 92-93 29.995073009413485 34.0 30.0 35.0 18.0 35.5 94-95 29.621699072996172 34.0 30.0 35.0 12.5 35.0 96-97 29.232416003036683 34.0 29.5 35.0 4.5 35.0 98-99 28.755268451525126 34.0 29.0 35.0 2.0 35.0 100-101 27.641073795066056 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3667578940895737 1101 2 0.3552698296074901 1101 3 0.3556723798084569 1101 4 0.2529006782356049 1101 5 0.32765284843367937 1101 6 0.24785333331320203 1101 7 0.3169164415802257 1101 8 0.22967019956755053 1101 9 0.3655704304258762 1101 10-11 0.4187588674460798 1101 12-13 0.404250206611394 1101 14-15 0.4506695095666515 1101 16-17 0.45005092252213785 1101 18-19 0.36549998275864226 1101 20-21 0.43817873299932586 1101 22-23 0.4857991424427368 1101 24-25 0.45174893630636603 1101 26-27 0.43766260048186467 1101 28-29 0.4844622452235967 1101 30-31 0.5204975038741608 1101 32-33 0.4943434381026748 1101 34-35 0.4898624776794662 1101 36-37 0.5015195881867314 1101 38-39 0.48541345329596197 1101 40-41 0.5291753017636438 1101 42-43 0.44110570980983965 1101 44-45 0.4552144598274239 1101 46-47 0.41326593083577023 1101 48-49 0.46226043319420995 1101 50-51 0.4679900424694452 1101 52-53 0.48703438872288274 1101 54-55 0.4101480065133103 1101 56-57 0.48663782562112345 1101 58-59 0.4347254401051259 1101 60-61 0.49745499183388375 1101 62-63 0.400807210125123 1101 64-65 0.452612355198454 1101 66-67 0.43340380576916715 1101 68-69 0.5041933462150823 1101 70-71 0.47745994300284167 1101 72-73 0.4597074706236626 1101 74-75 0.39295492966304835 1101 76-77 0.3266471934924233 1101 78-79 0.46734074704773576 1101 80-81 0.4622100306516401 1101 82-83 0.398399462854929 1101 84-85 0.4973296019837754 1101 86-87 0.3489278308258541 1101 88-89 0.3322036034057696 1101 90-91 0.4508109052211644 1101 92-93 0.4718406006617393 1101 94-95 0.530408432798275 1101 96-97 0.43022267939566916 1101 98-99 0.5049039859774744 1101 100-101 0.725690272626597 1102 1 0.24892564010028195 1102 2 0.2606031620536591 1102 3 0.32154678820970517 1102 4 0.23392693447429735 1102 5 0.27659642645307514 1102 6 0.15238555804831577 1102 7 0.17160898766332622 1102 8 0.15635376224346942 1102 9 0.3184141964571836 1102 10-11 0.2975770206532715 1102 12-13 0.14237792118993298 1102 14-15 0.39794311513022507 1102 16-17 0.3359445291192529 1102 18-19 0.3771387781073443 1102 20-21 0.3905862368856532 1102 22-23 0.3002334754529201 1102 24-25 0.27273252446602925 1102 26-27 0.31218444763397457 1102 28-29 0.3612920455432871 1102 30-31 0.2703275568133634 1102 32-33 0.33264820813130314 1102 34-35 0.3835007908553578 1102 36-37 0.3569654461027767 1102 38-39 0.3842354567882751 1102 40-41 0.29851860414165543 1102 42-43 0.26413520552229386 1102 44-45 0.3226645131347965 1102 46-47 0.3188874801896162 1102 48-49 0.24176673615088617 1102 50-51 0.20083448274423432 1102 52-53 0.29113394432102524 1102 54-55 0.33533603505421183 1102 56-57 0.3852329373115495 1102 58-59 0.4432102619751035 1102 60-61 0.3506872684901694 1102 62-63 0.28917622180616576 1102 64-65 0.33420788141555136 1102 66-67 0.35942787404341203 1102 68-69 0.327545658171573 1102 70-71 0.3730922647269921 1102 72-73 0.43329187537201364 1102 74-75 0.28803199857583195 1102 76-77 0.21539247295636343 1102 78-79 0.3434897610664578 1102 80-81 0.32919166217448037 1102 82-83 0.26495891593700804 1102 84-85 0.15282456434258052 1102 86-87 0.28793164052329345 1102 88-89 0.38152384843117204 1102 90-91 0.5446154726440682 1102 92-93 0.40115127320408206 1102 94-95 0.26263651694652523 1102 96-97 0.3567282551009008 1102 98-99 0.5836908888866326 1102 100-101 0.667459145954659 1103 1 0.23744169260301362 1103 2 0.24671570149513045 1103 3 0.30178511608848524 1103 4 0.15278503902841578 1103 5 0.23459967121264924 1103 6 0.2177652429631678 1103 7 0.18102822387156436 1103 8 0.13992705716722753 1103 9 0.345314264360546 1103 10-11 0.27124772735879077 1103 12-13 0.27544753574646563 1103 14-15 0.41330264731080746 1103 16-17 0.3991437609444475 1103 18-19 0.310214112954462 1103 20-21 0.348762482801952 1103 22-23 0.33118407359334867 1103 24-25 0.3344481060985274 1103 26-27 0.2957787357732897 1103 28-29 0.33353973588187813 1103 30-31 0.33979506048230945 1103 32-33 0.2607110003632016 1103 34-35 0.2924004340020545 1103 36-37 0.4096033844498379 1103 38-39 0.3880231547263264 1103 40-41 0.4580935865511364 1103 42-43 0.35216767394184245 1103 44-45 0.27847628446417616 1103 46-47 0.30289677791982683 1103 48-49 0.276944804657127 1103 50-51 0.3214451225098074 1103 52-53 0.3683336249883027 1103 54-55 0.2907850069887914 1103 56-57 0.34214499273614507 1103 58-59 0.36095608362271747 1103 60-61 0.41626686250760514 1103 62-63 0.35170295569712096 1103 64-65 0.24174221915329497 1103 66-67 0.2310473171605807 1103 68-69 0.25488935593550366 1103 70-71 0.1507123491188409 1103 72-73 0.25862979589334856 1103 74-75 0.31147995264992545 1103 76-77 0.18850935894056065 1103 78-79 0.34077014550008755 1103 80-81 0.4094895952302693 1103 82-83 0.35806719514188146 1103 84-85 0.426547098637041 1103 86-87 0.47397754986948115 1103 88-89 0.44572911754638156 1103 90-91 0.5247237266345017 1103 92-93 0.36404391125282487 1103 94-95 0.396319595034889 1103 96-97 0.4473420924587437 1103 98-99 0.5874107524577035 1103 100-101 0.8897056320215491 1104 1 0.14194994746991085 1104 2 0.1848269913740488 1104 3 0.18763827677246425 1104 4 0.1509792396970724 1104 5 0.18472424908032536 1104 6 0.17318588161099768 1104 7 0.19450917721125904 1104 8 0.1158300906213583 1104 9 0.23750683364244907 1104 10-11 0.2411874583333926 1104 12-13 0.19124486085729586 1104 14-15 0.22267875664012848 1104 16-17 0.254592281107314 1104 18-19 0.2547503884763884 1104 20-21 0.3261599987605379 1104 22-23 0.32390253260716406 1104 24-25 0.3003416637620049 1104 26-27 0.2775267610853476 1104 28-29 0.32605305853671496 1104 30-31 0.2667365421235033 1104 32-33 0.22696689879909115 1104 34-35 0.3017726783314174 1104 36-37 0.33272553854631326 1104 38-39 0.4175779887887927 1104 40-41 0.3593336331356838 1104 42-43 0.3278008293147181 1104 44-45 0.3495167198558278 1104 46-47 0.35032819409030225 1104 48-49 0.17385238846490125 1104 50-51 0.2463761263145159 1104 52-53 0.3207093631586986 1104 54-55 0.25692618033379944 1104 56-57 0.31428799369098215 1104 58-59 0.31664566680566253 1104 60-61 0.2962205348343403 1104 62-63 0.24550221735131572 1104 64-65 0.33482998188559066 1104 66-67 0.38002268188873245 1104 68-69 0.3713358404920939 1104 70-71 0.2329258512404877 1104 72-73 0.26640281621956063 1104 74-75 0.3026984646439672 1104 76-77 0.2623690263816947 1104 78-79 0.2982881769140917 1104 80-81 0.3050451485394916 1104 82-83 0.37202859770636465 1104 84-85 0.3353204166277095 1104 86-87 0.39671356668615587 1104 88-89 0.35557439030061744 1104 90-91 0.5289777834276457 1104 92-93 0.5184745152976085 1104 94-95 0.4954559043071889 1104 96-97 0.6033378133686433 1104 98-99 0.6590975644951449 1104 100-101 0.7471957343512905 1105 1 0.19505462237379945 1105 2 0.1526370373149568 1105 3 0.12033324798192879 1105 4 0.11416208669037786 1105 5 0.13541603457805707 1105 6 0.19401352192075905 1105 7 0.14393587862882384 1105 8 0.18084811690781066 1105 9 0.20261109823623968 1105 10-11 0.15647635956720762 1105 12-13 0.1912946626520835 1105 14-15 0.20694540313979104 1105 16-17 0.15596980548878747 1105 18-19 0.24864398793508968 1105 20-21 0.24410256196841118 1105 22-23 0.24467609609054364 1105 24-25 0.1845633469197807 1105 26-27 0.23982183619666841 1105 28-29 0.18858308032368853 1105 30-31 0.17301828866210656 1105 32-33 0.27469978738973566 1105 34-35 0.21946991513337366 1105 36-37 0.2625980130757526 1105 38-39 0.3410646871383278 1105 40-41 0.3674813496502054 1105 42-43 0.26957654849439905 1105 44-45 0.16482585778815206 1105 46-47 0.2454183551457234 1105 48-49 0.23566412943259962 1105 50-51 0.18807138661137657 1105 52-53 0.16477882266401167 1105 54-55 0.2333596329904708 1105 56-57 0.2343782897932627 1105 58-59 0.1646744851654347 1105 60-61 0.1487663771424721 1105 62-63 0.14157764540482276 1105 64-65 0.20530248962784015 1105 66-67 0.1558524141178239 1105 68-69 0.17588411399211878 1105 70-71 0.08011101883246852 1105 72-73 0.1728311045683526 1105 74-75 0.24011741547570864 1105 76-77 0.2257520041003609 1105 78-79 0.21859222459649885 1105 80-81 0.256223904281093 1105 82-83 0.21431973700054385 1105 84-85 0.3607112523888354 1105 86-87 0.36307482416578907 1105 88-89 0.36826787105748693 1105 90-91 0.3792167234242676 1105 92-93 0.3579144176177813 1105 94-95 0.338561501014766 1105 96-97 0.45026517796790344 1105 98-99 0.5179046552623703 1105 100-101 0.6486376862470742 1106 1 -0.04673095903723734 1106 2 -0.008528923718007064 1106 3 -0.1363228316776599 1106 4 0.04001759214300904 1106 5 0.006475776505119768 1106 6 0.12644442576345938 1106 7 0.04771651786698072 1106 8 0.034615362731500454 1106 9 -0.04192384421386919 1106 10-11 -0.055353995460393435 1106 12-13 -0.01761023633579839 1106 14-15 0.18745453888462293 1106 16-17 0.10173077950992848 1106 18-19 -0.008376359636820041 1106 20-21 0.09383330165864834 1106 22-23 0.05078392864525938 1106 24-25 0.1470087778054321 1106 26-27 0.2591407373680994 1106 28-29 0.15233280199317534 1106 30-31 0.12320193235714072 1106 32-33 0.10111876388330643 1106 34-35 0.08178845732245321 1106 36-37 0.06104367657302845 1106 38-39 -0.055495003492936235 1106 40-41 -0.0462804764169249 1106 42-43 -0.05390584756772654 1106 44-45 -0.02902373090070398 1106 46-47 0.021695230778497887 1106 48-49 0.04520846316481908 1106 50-51 -0.022171099818486084 1106 52-53 -0.13495762342714812 1106 54-55 -0.13739109216977852 1106 56-57 -0.06475955807720624 1106 58-59 -0.030087032891422893 1106 60-61 0.12362279744389326 1106 62-63 -0.01663699096323512 1106 64-65 0.0012014620224576333 1106 66-67 0.006666922655448104 1106 68-69 0.09341204107120404 1106 70-71 -0.03366801766924965 1106 72-73 -0.08240792182252932 1106 74-75 -0.060948342451759174 1106 76-77 -0.13047121900320136 1106 78-79 -0.06737095908198398 1106 80-81 0.09928683661306081 1106 82-83 0.1421351366078234 1106 84-85 0.27683645253224043 1106 86-87 0.3542886206717064 1106 88-89 0.25655658822265437 1106 90-91 0.2556913660208089 1106 92-93 0.20266062344975566 1106 94-95 0.3155867009403899 1106 96-97 0.24342407730764037 1106 98-99 0.3756871354990672 1106 100-101 0.2371837123583198 1107 1 0.06915445134471909 1107 2 0.11170433601848373 1107 3 0.051152398179397096 1107 4 0.11677526802184701 1107 5 0.06214475473039727 1107 6 0.04493262825207722 1107 7 0.059880541504554685 1107 8 0.04998106979799388 1107 9 -0.027150424049061428 1107 10-11 -0.08592770451953413 1107 12-13 -0.0291671850117865 1107 14-15 -0.01130726690225714 1107 16-17 0.12560041404436362 1107 18-19 0.02295641848245822 1107 20-21 -0.019530847207313684 1107 22-23 -0.07750514953612253 1107 24-25 -0.042652049711342954 1107 26-27 0.005057295899931091 1107 28-29 0.05801960519357152 1107 30-31 0.07545064228433063 1107 32-33 0.031111084247548604 1107 34-35 0.0881819654737015 1107 36-37 0.10817708590283814 1107 38-39 0.10226614745447193 1107 40-41 0.05458721413803147 1107 42-43 0.03424473335827116 1107 44-45 -1.8600428695236815E-4 1107 46-47 0.056202210041014666 1107 48-49 0.012591748259531244 1107 50-51 0.07220249867480533 1107 52-53 0.008105593783056975 1107 54-55 0.07298854322945658 1107 56-57 0.12442942649324351 1107 58-59 0.10115832223430488 1107 60-61 0.2534226653577605 1107 62-63 0.18013293960738963 1107 64-65 0.16632796252130788 1107 66-67 0.139469325842974 1107 68-69 0.2819816101810275 1107 70-71 0.30464671813835764 1107 72-73 0.07657156671621834 1107 74-75 0.1838519343588132 1107 76-77 0.18597700827868735 1107 78-79 0.24181128163109378 1107 80-81 0.3217780471025975 1107 82-83 0.3180888361051579 1107 84-85 0.32749976947513204 1107 86-87 0.2722344003185917 1107 88-89 0.2634872532514052 1107 90-91 0.2986623301558744 1107 92-93 0.3643758460150188 1107 94-95 0.2562013098251299 1107 96-97 0.2673792338396517 1107 98-99 0.32211295556117037 1107 100-101 0.5219684156074287 1108 1 0.01757446597112633 1108 2 0.06723759401400997 1108 3 0.01269681557451463 1108 4 0.04220324334055192 1108 5 -0.030702315133410707 1108 6 -0.0013676889409808268 1108 7 0.06807702478209876 1108 8 -0.019108155197770316 1108 9 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-1.0070990655677612 2316 90-91 -1.2846726202937546 2316 92-93 -1.2776619948918366 2316 94-95 -1.162281089288335 2316 96-97 -1.2433837353366535 2316 98-99 -1.3774859792496805 2316 100-101 -1.7817528731353072 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 154.0 3 99.0 4 282.0 5 789.0 6 1435.0 7 2462.0 8 4256.0 9 6501.0 10 8865.0 11 11212.0 12 13607.0 13 16247.0 14 18762.0 15 21482.0 16 24619.0 17 28741.0 18 33056.0 19 37381.0 20 40875.0 21 43399.0 22 48948.0 23 58339.0 24 71389.0 25 89911.0 26 114499.0 27 144777.0 28 182266.0 29 225261.0 30 275345.0 31 334373.0 32 408357.0 33 505192.0 34 650800.0 35 909282.0 36 1156218.0 37 1642986.0 38 1572683.0 39 357044.0 40 636.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.201722784391595 31.625132389265993 28.262688151890046 21.910456674452362 2 22.472124230209445 29.513369886775546 28.46721610852598 19.54728977448902 3 20.956289248145936 28.791352966555696 28.753493781537827 21.49886400376054 4 21.42195054019828 29.21993355276429 28.418993651450837 20.939122255586597 5 21.955774872151654 29.081042251609517 28.31772045115611 20.645462425082716 6 21.683801295610337 28.910524938794126 28.17745582401751 21.22821794157803 7 21.87336448799226 29.145517768825897 28.10478178883621 20.876335954345635 8 21.11714782675478 29.028612060658272 28.429252523611844 21.424987588975103 9 21.00362460209708 28.84290801490801 28.839564571059327 21.313902811935588 10-11 21.04791928470672 29.30744267006072 28.332537289157028 21.31210075607553 12-13 21.06788292760222 29.313164128909268 28.17391811932409 21.445034824164424 14-15 21.210967777922022 29.33261396088299 28.00894915806334 21.44746910313165 16-17 21.395441680542028 29.134164929798565 27.988905083167463 21.481488306491947 18-19 21.37464726317826 29.134082171438138 27.937561796357386 21.553708769026215 20-21 21.486469219054033 29.068530812945887 27.956809373814483 21.488190594185593 22-23 21.44756687454321 29.07190010979849 27.97458827771841 21.50594473793989 24-25 21.45276208740826 29.052146585997505 27.857805712091437 21.637285614502794 26-27 21.623074440750788 28.951602309266967 27.96838344084839 21.456939809133864 28-29 21.632584823896686 28.98606379820812 27.928748699240014 21.45260267865518 30-31 21.57914604983967 29.011035623206666 27.87184799929467 21.537970327658996 32-33 21.61696044605805 29.045751460896955 27.760741451239017 21.576546641805972 34-35 21.65595761923975 29.04060172743562 27.747030526923094 21.556410126401538 36-37 21.657935533967557 29.05040980167324 27.73781299815553 21.553841666203677 38-39 21.6519476369331 29.053857370074965 27.719108741177024 21.575086251814913 40-41 21.66203976815409 29.11939627195349 27.698536761276976 21.52002719861544 42-43 21.692506797113573 29.094702651419123 27.669619149225767 21.543171402241537 44-45 21.66191594039332 29.106097297492937 27.679980102563604 21.55200665955014 46-47 21.690441700396505 29.144896626633738 27.627078786624125 21.537582886345632 48-49 21.727309840580862 29.109098674846233 27.603012139964235 21.560579344608673 50-51 21.726362425547325 29.139956105137337 27.59506153275802 21.538619936557318 52-53 21.708224188030123 29.194912387823162 27.527211514576134 21.56965190957058 54-55 21.720109500035726 29.223344816142177 27.481055020650576 21.57549066317152 56-57 21.723889465745216 29.212559848077746 27.482231782956855 21.581318903220183 58-59 21.70628541887758 29.29075210571891 27.459578721045936 21.543383754357574 60-61 21.711018478513164 29.27595860035336 27.434678358581948 21.57834456255153 62-63 21.70089094555606 29.314085632798765 27.43647537716206 21.548548044483113 64-65 21.729372569476723 29.363752903110658 27.400597684242527 21.506276843170095 66-67 21.70120568874549 29.360283720029507 27.40876506421273 21.529745527012274 68-69 21.648398429961166 29.4349398413299 27.402250230097795 21.51441149861114 70-71 21.701764879482578 29.467298603510645 27.35542508121604 21.475511435790736 72-73 21.68281482790121 29.456150689663602 27.30663655798339 21.5543979244518 74-75 21.704364005578835 29.495665390395143 27.303044629521622 21.4969259745044 76-77 21.709053652787908 29.5281505275017 27.304571681417883 21.458224138292508 78-79 21.68775165433935 29.54465254183986 27.26839524945021 21.499200554370578 80-81 21.649024046722527 29.56168722973865 27.28898152435238 21.50030719918645 82-83 21.682316849386385 29.619424587923394 27.24628618504755 21.451972377642672 84-85 21.669019857784537 29.65288945956678 27.22770215094229 21.450388531706395 86-87 21.66969524509968 29.686969038981076 27.197894128769434 21.445441587149816 88-89 21.664854783404916 29.746118961501782 27.179242041341396 21.409784213751905 90-91 21.602317951806477 29.741174149448106 27.186195704521836 21.47031219422358 92-93 21.61090426902426 29.780041178623616 27.148975626840766 21.460078925511358 94-95 21.61153550068753 29.84844071888162 27.11256565315054 21.427458127280314 96-97 21.606944283906586 29.841468901647932 27.10323156045283 21.448355253992656 98-99 21.609186397176064 29.87891350428633 27.07599864497 21.435901453567602 100-101 21.668723947326185 29.859400733536923 27.004987956177136 21.466887362959756 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 163786.0 1 92367.5 2 15478.5 3 9051.5 4 7475.0 5 6368.0 6 5857.0 7 5623.5 8 5224.5 9 4943.0 10 4883.0 11 5473.0 12 9585.0 13 9456.0 14 5767.5 15 6013.5 16 6664.5 17 7827.5 18 9471.0 19 11297.5 20 14104.5 21 18445.0 22 23970.5 23 30908.0 24 40080.0 25 51839.5 26 66505.0 27 81738.5 28 95114.5 29 108855.0 30 123668.5 31 140133.5 32 157616.0 33 173848.0 34 191907.5 35 209145.5 36 226875.5 37 246416.0 38 261391.0 39 271873.0 40 285780.5 41 298228.5 42 304846.0 43 316522.5 44 329830.5 45 340433.5 46 345582.0 47 342998.0 48 340443.0 49 338966.5 50 333513.5 51 321352.0 52 307614.5 53 290609.0 54 268259.5 55 248591.5 56 226775.5 57 201706.5 58 181098.0 59 157847.0 60 128508.5 61 99082.5 62 71392.5 63 49169.5 64 33742.0 65 22268.5 66 13791.5 67 8665.0 68 4908.5 69 2310.5 70 1134.5 71 556.5 72 277.5 73 162.5 74 102.5 75 67.0 76 51.0 77 43.5 78 32.0 79 21.0 80 15.0 81 11.5 82 12.0 83 12.5 84 9.5 85 6.5 86 6.0 87 6.0 88 4.5 89 1.5 90 0.5 91 1.5 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1485015773740887 2 0.0 3 0.0 4 0.0033324027617012025 5 3.862056180779539E-4 6 9.158590371562908E-4 7 1.1034446230798683E-5 8 2.5379226330836976E-4 9 1.9862003215437632E-4 10-11 1.0482723919258751E-4 12-13 1.1034446230798683E-5 14-15 1.6551669346198027E-5 16-17 1.6551669346198027E-5 18-19 1.6551669346198027E-5 20-21 8.827556984638947E-5 22-23 2.1517170150057435E-4 24-25 0.0 26-27 5.517223115399342E-6 28-29 0.005737912040015316 30-31 0.0016662013808506012 32-33 3.5861950250095726E-4 34-35 2.372405939621717E-4 36-37 4.027572874241519E-4 38-39 0.0012358579778494526 40-41 0.0033544716541628002 42-43 4.799984110397428E-4 44-45 6.179289889247263E-4 46-47 0.006322737690247646 48-49 0.00662618496159461 50-51 0.007166872826903745 52-53 0.009191693710255305 54-55 0.004772397994820431 56-57 0.0 58-59 8.275834673099013E-5 60-61 9.434451527332876E-4 62-63 8.772384753484954E-4 64-65 5.793084271169309E-4 66-67 8.275834673099013E-4 68-69 8.717212522330961E-4 70-71 0.0013958574481960336 72-73 0.002753094334584272 74-75 7.117217818865152E-4 76-77 0.0 78-79 0.0 80-81 6.620667738479211E-5 82-83 1.7655113969277893E-4 84-85 1.4896502411578221E-4 86-87 0.002731025442122674 88-89 0.001500684687388621 90-91 5.131017497321388E-4 92-93 6.731012200787197E-4 94-95 4.027572874241519E-4 96-97 0.001241375200964852 98-99 0.0 100-101 5.517223115399342E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9062530.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.95091988269994 #Duplication Level Percentage of deduplicated Percentage of total 1 91.93941474420802 66.15125464322833 2 4.791555647003238 6.895136729420568 3 1.1656461454956906 2.5160793727841524 4 0.5419660731388501 1.5597983003021958 5 0.3085429007600637 1.109997276648159 6 0.2151301142770983 0.9287285770024547 7 0.13661065343402987 0.6880483526248619 8 0.1001397944982261 0.5764120264809521 9 0.07550789241702659 0.4889576086028105 >10 0.6911252122383815 10.995557647469841 >50 0.03250673837676861 1.4412512347560378 >100 0.0014862149866308146 0.17288967218290308 >500 9.19672915384564E-5 0.03943810210284197 >1k 9.196729152686376E-5 0.22031738411900445 >5k 9.196729152686376E-5 0.5011815189419851 >10k+ 9.196729152686376E-5 5.714951553332914 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 143120 1.5792499445519077 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29612 0.32675202178641066 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 9945 0.10973756776529292 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.1034446230798683E-5 3 0.0 0.0 0.0 0.0 3.3103338692396054E-5 4 1.1034446230798683E-5 1.1034446230798683E-5 0.0 0.0 3.3103338692396054E-5 5 1.1034446230798683E-5 1.1034446230798683E-5 0.0 0.0 3.3103338692396054E-5 6 1.1034446230798683E-5 1.1034446230798683E-5 0.0 0.0 4.4137784923194734E-5 7 1.1034446230798683E-5 1.1034446230798683E-5 0.0 0.0 4.4137784923194734E-5 8 1.1034446230798683E-5 1.1034446230798683E-5 0.0 1.1034446230798683E-5 4.4137784923194734E-5 9 1.1034446230798683E-5 1.1034446230798683E-5 0.0 2.2068892461597367E-5 4.4137784923194734E-5 10-11 1.1034446230798683E-5 1.6551669346198027E-5 0.0 2.758611557699671E-5 4.4137784923194734E-5 12-13 2.2068892461597367E-5 2.2068892461597367E-5 0.0 3.3103338692396054E-5 4.965500803859408E-5 14-15 2.2068892461597367E-5 2.2068892461597367E-5 0.0 4.4137784923194734E-5 5.517223115399342E-5 16-17 2.2068892461597367E-5 2.2068892461597367E-5 0.0 4.4137784923194734E-5 6.068945426939276E-5 18-19 2.758611557699671E-5 2.2068892461597367E-5 0.0 4.4137784923194734E-5 7.172390050019144E-5 20-21 6.068945426939276E-5 2.2068892461597367E-5 0.0 4.4137784923194734E-5 7.724112361559079E-5 22-23 6.620667738479211E-5 2.2068892461597367E-5 0.0 4.4137784923194734E-5 7.724112361559079E-5 24-25 6.620667738479211E-5 2.2068892461597367E-5 0.0 4.4137784923194734E-5 7.724112361559079E-5 26-27 6.620667738479211E-5 2.2068892461597367E-5 0.0 4.4137784923194734E-5 8.275834673099013E-5 28-29 6.620667738479211E-5 3.3103338692396054E-5 0.0 4.4137784923194734E-5 8.827556984638947E-5 30-31 6.620667738479211E-5 4.4137784923194734E-5 0.0 4.4137784923194734E-5 8.827556984638947E-5 32-33 6.620667738479211E-5 4.4137784923194734E-5 0.0 4.4137784923194734E-5 9.931001607718816E-5 34-35 6.620667738479211E-5 4.4137784923194734E-5 0.0 4.4137784923194734E-5 9.931001607718816E-5 36-37 6.620667738479211E-5 4.4137784923194734E-5 0.0 4.4137784923194734E-5 9.931001607718816E-5 38-39 7.172390050019144E-5 4.4137784923194734E-5 0.0 5.517223115399342E-5 1.048272391925875E-4 40-41 7.724112361559079E-5 4.4137784923194734E-5 0.0 5.517223115399342E-5 1.1034446230798684E-4 42-43 8.827556984638947E-5 4.4137784923194734E-5 0.0 5.517223115399342E-5 1.1034446230798684E-4 44-45 1.048272391925875E-4 4.4137784923194734E-5 0.0 5.517223115399342E-5 1.1034446230798684E-4 46-47 1.4896502411578224E-4 4.4137784923194734E-5 0.0 6.068945426939276E-5 1.1034446230798684E-4 48-49 1.8758558592357763E-4 4.4137784923194734E-5 0.0 6.620667738479211E-5 1.2689613165418488E-4 50-51 2.0413725526977566E-4 4.4137784923194734E-5 0.0 6.620667738479211E-5 1.3241335476958421E-4 52-53 2.5379226330836976E-4 4.965500803859408E-5 0.0 6.620667738479211E-5 1.3241335476958421E-4 54-55 2.8689560200076576E-4 5.517223115399342E-5 0.0 6.620667738479211E-5 1.3241335476958421E-4 56-57 3.3655061003935987E-4 5.517223115399342E-5 0.0 6.620667738479211E-5 1.3241335476958421E-4 58-59 4.248261798857493E-4 5.517223115399342E-5 0.0 6.620667738479211E-5 1.3793057788498355E-4 60-61 4.744811879243434E-4 5.517223115399342E-5 0.0 6.620667738479211E-5 1.434478010003829E-4 62-63 5.517223115399342E-4 5.517223115399342E-5 0.0 6.620667738479211E-5 1.434478010003829E-4 64-65 6.951701125403171E-4 5.517223115399342E-5 0.0 6.620667738479211E-5 1.5448224723118157E-4 66-67 7.613767899251092E-4 5.517223115399342E-5 0.0 7.172390050019144E-5 1.5448224723118157E-4 68-69 8.386179135407E-4 5.517223115399342E-5 0.0 7.724112361559079E-5 1.710339165773796E-4 70-71 8.772384753484954E-4 5.517223115399342E-5 0.0 8.827556984638947E-5 1.8206836280817827E-4 72-73 0.0010372379456950764 5.517223115399342E-5 0.0 8.827556984638947E-5 1.8758558592357763E-4 74-75 0.001208271862272456 5.517223115399342E-5 0.0 8.827556984638947E-5 1.8758558592357763E-4 76-77 0.0013627541095036374 5.517223115399342E-5 0.0 8.827556984638947E-5 1.8758558592357763E-4 78-79 0.0015006846873886212 5.517223115399342E-5 0.0 8.827556984638947E-5 1.8758558592357763E-4 80-81 0.0018206836280817828 5.517223115399342E-5 0.0 8.827556984638947E-5 1.9862003215437632E-4 82-83 0.002355854270275519 5.517223115399342E-5 0.0 9.931001607718816E-5 2.1517170150057432E-4 84-85 0.0030124038210080405 5.517223115399342E-5 0.0 9.931001607718816E-5 2.4275781707757104E-4 86-87 0.0038013667265101468 5.517223115399342E-5 0.0 9.931001607718816E-5 2.4827504019297043E-4 88-89 0.005070328043051995 5.517223115399342E-5 0.0 9.931001607718816E-5 2.5379226330836976E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 405 0.0 30.49318 3 CGCGTAG 285 0.0 23.333094 54-55 ATCAACG 6230 0.0 20.737007 10-11 CAACGCA 6235 0.0 20.6442 12-13 CGTACGC 290 0.0 20.47297 20-21 TCGCGTA 355 0.0 18.063993 52-53 CTCGCGT 350 0.0 17.643454 52-53 ACTCGCG 370 0.0 17.332338 50-51 GGTATCA 8405 0.0 17.179127 8 TCAACGC 6270 0.0 17.157969 10-11 AGTGGTA 8555 0.0 16.71154 5 CGCTAGT 375 0.0 16.466316 90-91 GTATCAA 8950 0.0 16.186092 9 AACGCAG 6805 0.0 15.774133 12-13 ACGCAGA 8255 0.0 15.678952 14-15 TGGTATC 9385 0.0 15.638294 7 AAGCAGT 9785 0.0 15.459338 1 ACGTACG 405 0.0 14.659657 20-21 GTGGTAT 10440 0.0 13.921658 6 CGTAGCG 430 0.0 13.807465 56-57 >>END_MODULE