FastQCFastQC Report
Sat 18 Jun 2016
SRR578588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9064141
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109858712.120144644704887No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT237250.261745707618626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCACTCGGCCAGCTTC226890.2503160531152373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126630.1397043580853387No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG95700.10558088185080086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2364600.091.304085
AAGCAGT2384250.091.121261
GGTATCA2379150.091.0401848
GTATCAA2384050.090.987579
TGGTATC2377050.090.925277
GTGGTAT2386900.090.644656
GCAGTGG2402750.090.081863
AGCAGTG2408050.090.0701452
CAGTGGT2406750.089.706514
TCAACGC2373600.046.26161212-13
CAACGCA2370300.046.12658312-13
AACGCAG2382250.046.1113114-15
ATCAACG2371600.046.1107310-11
CGCAGAG2384300.046.039716-17
ACGCAGA2381550.045.9622814-15
TATCAAC2388550.045.88993510-11
CAGAGTA2377450.045.64184618-19
AGAGTAC2333350.045.5700218-19
GAGTACT1412100.045.3135120-21
GCAGAGT2407850.045.2836116-17
Produced by FastQC (version 0.11.5)