##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578588_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9064141 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.65657815781992 31.0 31.0 33.0 27.0 34.0 2 30.952966640744005 31.0 31.0 34.0 27.0 34.0 3 31.040316230738245 31.0 31.0 34.0 27.0 34.0 4 34.49048067544404 37.0 35.0 37.0 30.0 37.0 5 34.54488373470801 37.0 35.0 37.0 30.0 37.0 6 34.527778197625125 37.0 35.0 37.0 30.0 37.0 7 34.444643458216284 37.0 35.0 37.0 30.0 37.0 8 34.39345669931657 37.0 35.0 37.0 30.0 37.0 9 35.82709580532783 39.0 35.0 39.0 30.0 39.0 10-11 35.810135621235375 39.0 35.0 39.0 30.0 39.0 12-13 35.85817288146775 39.0 35.0 39.0 30.0 39.0 14-15 36.87955775401111 39.5 36.0 41.0 31.0 41.0 16-17 36.85052334247669 40.0 36.0 41.0 30.5 41.0 18-19 36.843917421408165 40.0 36.0 41.0 30.0 41.0 20-21 36.733625061657804 39.5 36.0 41.0 30.0 41.0 22-23 36.430184503970096 39.0 36.0 40.5 29.5 41.0 24-25 36.31219659976605 39.0 36.0 40.0 29.0 41.0 26-27 36.24987387111476 39.0 36.0 40.0 29.0 41.0 28-29 35.9277171438529 39.0 35.0 41.0 27.0 41.0 30-31 35.66507129577971 38.5 34.5 40.5 26.5 41.0 32-33 35.31930383695487 38.0 34.0 40.0 25.5 41.0 34-35 35.09639705516496 38.0 34.0 40.0 25.0 41.0 36-37 35.113240956865084 38.0 34.0 40.0 25.0 41.0 38-39 34.44482036411393 37.0 33.5 40.0 25.0 41.0 40-41 34.42058701425761 37.0 33.0 40.0 25.0 41.0 42-43 34.24385653312322 37.0 33.0 40.0 24.0 41.0 44-45 34.07360576142847 37.0 33.0 40.0 23.0 41.0 46-47 33.873907742609035 37.0 33.0 40.0 23.0 41.0 48-49 33.521477104118304 37.0 33.0 40.0 21.0 41.0 50-51 32.25449190386602 36.0 31.5 39.0 16.5 40.5 52-53 31.882919738340345 36.0 31.0 39.0 10.0 40.0 54-55 31.610495302312707 37.0 30.5 40.0 2.0 41.0 56-57 31.144379428784262 36.5 30.0 40.0 2.0 41.0 58-59 30.822897613794844 36.0 29.0 40.0 2.0 41.0 60-61 30.515952697558433 36.0 29.0 39.5 2.0 41.0 62-63 30.17337517145861 35.0 28.0 39.0 2.0 41.0 64-65 29.840879571489456 35.0 28.0 39.0 2.0 41.0 66-67 29.537101199109767 35.0 28.0 39.0 2.0 40.0 68-69 29.201369495465705 35.0 27.5 38.0 2.0 40.0 70-71 28.80179837228922 35.0 26.0 37.5 2.0 40.0 72-73 28.41362661944469 34.5 26.0 37.0 2.0 39.5 74-75 28.018862901625205 34.0 26.0 36.5 2.0 39.0 76-77 27.618260847883988 34.0 25.5 36.0 2.0 39.0 78-79 27.219645082749707 34.0 25.0 35.5 2.0 38.0 80-81 26.827773917020927 34.0 24.0 35.0 2.0 37.0 82-83 26.460302305535627 33.5 23.0 35.0 2.0 37.0 84-85 26.059061912209884 33.0 20.5 35.0 2.0 36.0 86-87 25.691362369583615 33.0 20.0 35.0 2.0 36.0 88-89 25.29583018401854 33.0 18.0 35.0 2.0 35.5 90-91 24.917816205639344 32.0 15.5 35.0 2.0 35.0 92-93 24.551310984681287 32.0 8.5 35.0 2.0 35.0 94-95 24.097384131601657 32.0 2.0 35.0 2.0 35.0 96-97 23.60559919577597 32.0 2.0 35.0 2.0 35.0 98-99 22.99537600970682 31.0 2.0 34.0 2.0 35.0 100-101 21.408488460186135 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4508245917557474 1101 2 0.4697688025899218 1101 3 0.4784726565141888 1101 4 0.6075954438849678 1101 5 0.5765114905133046 1101 6 0.44983972430201646 1101 7 0.47272719138331354 1101 8 0.5515791565785122 1101 9 0.5464592325584405 1101 10-11 0.5583625078551506 1101 12-13 0.5275514087138262 1101 14-15 0.6672909695653573 1101 16-17 0.5935912491636017 1101 18-19 0.5645658234812103 1101 20-21 0.5875638473759892 1101 22-23 0.6413331189214446 1101 24-25 0.8444231194759126 1101 26-27 0.7316366494932609 1101 28-29 0.6976607203292033 1101 30-31 0.7927610197334118 1101 32-33 0.9025549416106529 1101 34-35 0.8327809874689578 1101 36-37 0.7870004606295353 1101 38-39 0.961406313732482 1101 40-41 0.858483925368752 1101 42-43 1.1278759370260971 1101 44-45 1.0327897934902452 1101 46-47 0.9688460210865131 1101 48-49 0.9627408870843155 1101 50-51 1.0739363229231316 1101 52-53 1.1360013356189462 1101 54-55 0.9717997310399298 1101 56-57 1.0576835393016317 1101 58-59 1.1539809829867202 1101 60-61 1.2284943028135231 1101 62-63 1.2273253560348287 1101 64-65 1.1501124079095284 1101 66-67 1.1026648249329014 1101 68-69 1.0881979227940448 1101 70-71 1.0774679048531866 1101 72-73 1.242910653405307 1101 74-75 1.1974635053432188 1101 76-77 1.1226608756815608 1101 78-79 1.1625486143337227 1101 80-81 1.2545933080868963 1101 82-83 1.3454212705844917 1101 84-85 1.3964133752439345 1101 86-87 1.3591683218364743 1101 88-89 1.4147509331463617 1101 90-91 1.6240089836407634 1101 92-93 1.6213663144893964 1101 94-95 1.6214435818148125 1101 96-97 1.8433297245657911 1101 98-99 2.030745250587888 1101 100-101 2.3592142289008358 1102 1 0.46289982988156453 1102 2 0.44605917673683493 1102 3 0.43760317849137564 1102 4 0.5317919916595955 1102 5 0.5190433289744476 1102 6 0.4186804658700325 1102 7 0.48152502748207837 1102 8 0.5769168259887536 1102 9 0.5416268813408323 1102 10-11 0.5883550025933388 1102 12-13 0.5853334743693495 1102 14-15 0.7177635985610635 1102 16-17 0.7180896204655838 1102 18-19 0.6074642476431436 1102 20-21 0.6421819830790199 1102 22-23 0.655096341578151 1102 24-25 0.7998576984650683 1102 26-27 0.9152160385347159 1102 28-29 0.858361136317761 1102 30-31 0.8960560575729559 1102 32-33 0.8974420894779556 1102 34-35 0.9819202448389177 1102 36-37 0.770488929569737 1102 38-39 0.9245391885885894 1102 40-41 0.8629443563827195 1102 42-43 1.0339247229987407 1102 44-45 1.0670871370618187 1102 46-47 1.0531990888465614 1102 48-49 1.163395908083423 1102 50-51 0.9657481010280335 1102 52-53 1.031777764487419 1102 54-55 1.0892779954111091 1102 56-57 0.8655130213343689 1102 58-59 0.959055355349804 1102 60-61 1.015130736695422 1102 62-63 1.0396677094131874 1102 64-65 0.9833392693398331 1102 66-67 0.992931849330791 1102 68-69 1.1631350412349732 1102 70-71 1.12822478829316 1102 72-73 1.0942421591741507 1102 74-75 1.2671528627546813 1102 76-77 1.1736973425072534 1102 78-79 1.1075492163099732 1102 80-81 1.248231923070815 1102 82-83 1.385644795108103 1102 84-85 1.4343031606424148 1102 86-87 1.5426177746052865 1102 88-89 1.661537726092785 1102 90-91 1.584555734635952 1102 92-93 1.5820131275147027 1102 94-95 1.6327657529094743 1102 96-97 1.8908187662496267 1102 98-99 2.075027617000554 1102 100-101 2.2836378940643733 1103 1 0.3535628291934714 1103 2 0.29661691619673647 1103 3 0.10783713623578706 1103 4 0.2521917307846948 1103 5 0.284406369603289 1103 6 0.2043294308285155 1103 7 0.36497023352912805 1103 8 0.2519139226499334 1103 9 0.4644624948346703 1103 10-11 0.36981387727584547 1103 12-13 0.2445562689105074 1103 14-15 0.4860044178710581 1103 16-17 0.48032982300563276 1103 18-19 0.4946829625407574 1103 20-21 0.5580102647984759 1103 22-23 0.6168319991088183 1103 24-25 0.5031549741401946 1103 26-27 0.654214304456282 1103 28-29 0.6078646458111194 1103 30-31 0.5100398312598813 1103 32-33 0.7012694898738729 1103 34-35 0.7123562294709345 1103 36-37 0.743406905429417 1103 38-39 0.4752076854104743 1103 40-41 0.5970514258439934 1103 42-43 0.38631497801412706 1103 44-45 0.559391760458908 1103 46-47 0.6956002268575432 1103 48-49 0.7266758654769845 1103 50-51 0.6979790314730394 1103 52-53 0.5621480583268301 1103 54-55 0.6021256732928322 1103 56-57 0.6288288411995815 1103 58-59 0.7945053131865727 1103 60-61 0.7551602137784172 1103 62-63 0.7000040946032335 1103 64-65 0.7568937857267457 1103 66-67 0.7053809650770049 1103 68-69 0.7617055590057049 1103 70-71 0.8132251346392252 1103 72-73 0.7357959967619152 1103 74-75 0.9388814774261931 1103 76-77 0.9023902476277073 1103 78-79 0.9419405439219446 1103 80-81 0.8530883895328216 1103 82-83 0.9940690173777433 1103 84-85 0.8973569266617787 1103 86-87 1.0521677414333546 1103 88-89 1.1504152478516865 1103 90-91 0.9963610453930016 1103 92-93 0.9825719260695571 1103 94-95 1.0305550426172907 1103 96-97 1.2260217079213298 1103 98-99 1.3328752537903732 1103 100-101 1.700273423828559 1104 1 0.16798541177436022 1104 2 0.21606357814264854 1104 3 0.2167668874332982 1104 4 0.14130197446716153 1104 5 0.08925964459655944 1104 6 0.14753409427520836 1104 7 0.30973457751468914 1104 8 0.24857767701104905 1104 9 0.3005148646645921 1104 10-11 0.3650994593670447 1104 12-13 0.25744352628198897 1104 14-15 0.3103785246563717 1104 16-17 0.4987932765067953 1104 18-19 0.6106194507934717 1104 20-21 0.4690477090624654 1104 22-23 0.3046227922306102 1104 24-25 0.40964742504494467 1104 26-27 0.49935733623681955 1104 28-29 0.6873826768577018 1104 30-31 0.5634849419333605 1104 32-33 0.7390709868928624 1104 34-35 0.697561817104905 1104 36-37 0.8042720187098809 1104 38-39 0.6143228729664756 1104 40-41 0.6355224184587129 1104 42-43 0.679563977789833 1104 44-45 0.9848063764749497 1104 46-47 0.7509760375764714 1104 48-49 0.7904990944018166 1104 50-51 0.7905093082947232 1104 52-53 0.5326053725000115 1104 54-55 0.5545294174843747 1104 56-57 0.6288230342032364 1104 58-59 0.7640067452658528 1104 60-61 0.6178437213216377 1104 62-63 0.6328696153716145 1104 64-65 0.7463040203669777 1104 66-67 0.5350363500826241 1104 68-69 0.6147958263858868 1104 70-71 0.8062107469103594 1104 72-73 0.7868438630870891 1104 74-75 0.8329300342496175 1104 76-77 0.8902826719928996 1104 78-79 0.902734898746445 1104 80-81 0.7950592101101392 1104 82-83 0.8392413424896823 1104 84-85 0.8395495345726793 1104 86-87 0.9635263026134986 1104 88-89 1.024189384464652 1104 90-91 1.1535953239338887 1104 92-93 1.0981362674716202 1104 94-95 1.2006363288229487 1104 96-97 1.4129386449744281 1104 98-99 1.5962952458285038 1104 100-101 1.872242893941614 1105 1 -0.025264884699453205 1105 2 0.14276346535922002 1105 3 0.1640146669641922 1105 4 0.15213655732088682 1105 5 0.16714378684012843 1105 6 0.22742499771836577 1105 7 0.32464242750256744 1105 8 0.2876579912950632 1105 9 0.22693989126377545 1105 10-11 0.3474972902093185 1105 12-13 0.2638001280316544 1105 14-15 0.14697107166036005 1105 16-17 0.30888793039812157 1105 18-19 0.15103374482291798 1105 20-21 0.1748308136113934 1105 22-23 0.2834850295228719 1105 24-25 0.5285726584863255 1105 26-27 0.5175222613164081 1105 28-29 0.5923582404482559 1105 30-31 0.5364378791650992 1105 32-33 0.6351978559116773 1105 34-35 0.5344712608663329 1105 36-37 0.7902622847887741 1105 38-39 0.5785502629373127 1105 40-41 0.5650490619106705 1105 42-43 0.8380831908895487 1105 44-45 0.832550083754775 1105 46-47 0.6382057020856848 1105 48-49 0.7057115322221463 1105 50-51 0.760637434333475 1105 52-53 0.6950707863077845 1105 54-55 0.8580979606331027 1105 56-57 0.8777893022329799 1105 58-59 0.9212740477968744 1105 60-61 0.9222121994420895 1105 62-63 0.9130584586665123 1105 64-65 1.0267032766059891 1105 66-67 0.9528020769841561 1105 68-69 0.9934076467278388 1105 70-71 0.9321211210987812 1105 72-73 0.8470825807525735 1105 74-75 0.8398488984029235 1105 76-77 0.7291161110455775 1105 78-79 0.9257307865784661 1105 80-81 0.9111197154437676 1105 82-83 1.0268598412943533 1105 84-85 1.1223022630752446 1105 86-87 1.115023128855892 1105 88-89 1.0033916267791945 1105 90-91 1.1977449412395416 1105 92-93 1.3318879494252585 1105 94-95 1.4847826366534846 1105 96-97 1.6389010417892038 1105 98-99 1.9189228239168763 1105 100-101 2.3326232513358924 1106 1 0.2728425971278199 1106 2 0.32199866676351974 1106 3 0.25006015749126576 1106 4 0.11638082362298974 1106 5 0.2840022568696199 1106 6 0.2826603459207746 1106 7 0.350896786002032 1106 8 0.3228436934813388 1106 9 0.23215025909238562 1106 10-11 0.1567189392080479 1106 12-13 0.41184432758541334 1106 14-15 0.2873429395151845 1106 16-17 0.41273330558874477 1106 18-19 0.38148439834728975 1106 20-21 0.30486385065110255 1106 22-23 0.42952660083921756 1106 24-25 0.5802873103097639 1106 26-27 0.380536909711104 1106 28-29 0.3631286645816303 1106 30-31 0.48255253939669984 1106 32-33 0.4162787390826139 1106 34-35 0.3913237199844062 1106 36-37 0.5133944555186432 1106 38-39 0.5313316131915542 1106 40-41 0.38423331702141894 1106 42-43 0.5118384810917647 1106 44-45 0.40035728822207517 1106 46-47 0.6139680026440075 1106 48-49 0.7770994502901942 1106 50-51 0.631255638711238 1106 52-53 0.5115010435105809 1106 54-55 0.7078143548512585 1106 56-57 0.534091877247473 1106 58-59 0.6997750691521532 1106 60-61 0.5833199829635909 1106 62-63 0.7428003289030762 1106 64-65 0.8346581716823884 1106 66-67 0.810372227209271 1106 68-69 0.8762528983806206 1106 70-71 0.959693302720197 1106 72-73 0.8369893495982268 1106 74-75 0.825796431634803 1106 76-77 0.949434358538106 1106 78-79 1.0846079625688887 1106 80-81 1.0966474210301769 1106 82-83 1.1726053238210419 1106 84-85 1.254080388423251 1106 86-87 1.2324683892729134 1106 88-89 1.219172899671527 1106 90-91 1.0856750299633724 1106 92-93 1.2677282528447655 1106 94-95 1.3180787841968318 1106 96-97 1.5755321424608297 1106 98-99 1.7460430155538766 1106 100-101 1.944309552057554 1107 1 0.04155003795030865 1107 2 0.08759992088765145 1107 3 0.11948011098638744 1107 4 0.03763126177123155 1107 5 0.10249822489277705 1107 6 0.15484283827336753 1107 7 0.12598804977362477 1107 8 0.20314694436789438 1107 9 0.11659293045148189 1107 10-11 0.14812579427007222 1107 12-13 0.2041083544347373 1107 14-15 0.20137391019051165 1107 16-17 0.15381685530864075 1107 18-19 0.11641912364559914 1107 20-21 0.09827780399545816 1107 22-23 0.3249772506639559 1107 24-25 0.3319632624416542 1107 26-27 0.2795937643029731 1107 28-29 0.3866066718575425 1107 30-31 0.4111435861118977 1107 32-33 0.3792832267204247 1107 34-35 0.364965638802083 1107 36-37 0.2421816061239852 1107 38-39 0.3140446414350464 1107 40-41 0.18679779426413035 1107 42-43 0.26604149458273696 1107 44-45 0.21253091774160282 1107 46-47 0.3698024404915259 1107 48-49 0.4049854727946922 1107 50-51 0.320336918395455 1107 52-53 0.3248778313574654 1107 54-55 0.57984802489635 1107 56-57 0.3711368568410016 1107 58-59 0.339162635847746 1107 60-61 0.3434619545561475 1107 62-63 0.3705606793591336 1107 64-65 0.295799991127371 1107 66-67 0.36765399502978013 1107 68-69 0.27788503623861516 1107 70-71 0.29709519269303186 1107 72-73 0.32446281874068816 1107 74-75 0.43901695495772586 1107 76-77 0.4803990604985344 1107 78-79 0.4574913049285314 1107 80-81 0.5030921041110012 1107 82-83 0.569448061209858 1107 84-85 0.6487121907373705 1107 86-87 0.6738022744981471 1107 88-89 0.7511682478623243 1107 90-91 0.7229077011769078 1107 92-93 0.7556088692545195 1107 94-95 0.7456664695054371 1107 96-97 0.9227543613186313 1107 98-99 1.1239111014248344 1107 100-101 1.444239302995559 1108 1 0.1676048524375524 1108 2 0.12254232542356291 1108 3 -0.04408057855702907 1108 4 0.14498208628880604 1108 5 0.23584735887933306 1108 6 0.25643221676327954 1108 7 0.15822647677373425 1108 8 0.045565930330333515 1108 9 0.05707528980539678 1108 10-11 0.10869513068300307 1108 12-13 0.18680433479216418 1108 14-15 0.0759716362736782 1108 16-17 0.21647672413264019 1108 18-19 0.04299268619180907 1108 20-21 0.08676074571198455 1108 22-23 0.2100697596065757 1108 24-25 0.3174135764173087 1108 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2315 84-85 -1.703372211595564 2315 86-87 -1.7484758056074234 2315 88-89 -1.9897075444187955 2315 90-91 -2.0548058330856023 2315 92-93 -1.9408358986818577 2315 94-95 -2.053933658835586 2315 96-97 -2.103952360112821 2315 98-99 -2.193896261783337 2315 100-101 -2.34330118315966 2316 1 -1.1535594117904537 2316 2 -0.9945872472049366 2316 3 -2.2444960334829105 2316 4 -1.2936914358549387 2316 5 -1.1723801486063863 2316 6 -2.6453681462152794 2316 7 -6.795850094386854 2316 8 -7.7379028734418895 2316 9 -4.380292516228433 2316 10-11 -2.1179991781989713 2316 12-13 -2.2066184549776295 2316 14-15 -2.248656605828323 2316 16-17 -2.1480568574563748 2316 18-19 -1.6305937192599202 2316 20-21 -2.7196167857189693 2316 22-23 -3.191362578032148 2316 24-25 -1.8849258908293578 2316 26-27 -2.2319359564722205 2316 28-29 -3.109996772592929 2316 30-31 -3.6486642970417975 2316 32-33 -3.5350355863640175 2316 34-35 -2.6621310673192013 2316 36-37 -3.7084733457558876 2316 38-39 -3.2025076607109355 2316 40-41 -3.8512599724244723 2316 42-43 -3.086705861791856 2316 44-45 -2.736940842516489 2316 46-47 -2.4840739075591785 2316 48-49 -2.6306585845227595 2316 50-51 -2.2454346662552496 2316 52-53 -2.6377686608508206 2316 54-55 -4.027027646687209 2316 56-57 -2.669053061701181 2316 58-59 -2.077851257456235 2316 60-61 -2.7798112155198353 2316 62-63 -2.4966291068236224 2316 64-65 -4.124482858360544 2316 66-67 -3.038677474400803 2316 68-69 -2.914082084906017 2316 70-71 -3.232888110736994 2316 72-73 -2.8963489897145074 2316 74-75 -2.592941412719995 2316 76-77 -3.1680588675952244 2316 78-79 -4.234424355915177 2316 80-81 -3.171935162217011 2316 82-83 -2.737826578413763 2316 84-85 -2.839243024295534 2316 86-87 -4.071577624845876 2316 88-89 -4.4979780480907685 2316 90-91 -3.8380127248039173 2316 92-93 -3.955423670236282 2316 94-95 -4.092618801894314 2316 96-97 -4.380865356138457 2316 98-99 -4.655303432435542 2316 100-101 -4.707977862395158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81613.0 3 15319.0 4 13571.0 5 13283.0 6 15405.0 7 19023.0 8 23978.0 9 28356.0 10 33349.0 11 39430.0 12 41532.0 13 46976.0 14 53266.0 15 63671.0 16 80679.0 17 107186.0 18 150545.0 19 216600.0 20 276744.0 21 207896.0 22 129655.0 23 116360.0 24 121573.0 25 130919.0 26 144584.0 27 163991.0 28 189559.0 29 221350.0 30 261705.0 31 311565.0 32 377439.0 33 466756.0 34 598512.0 35 821520.0 36 1160243.0 37 1246649.0 38 926257.0 39 146949.0 40 133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.647576530978558 46.70898732412693 25.321580221582014 17.321855923312505 2 16.778126331212302 48.63155378906716 18.634967656614627 15.955352223105908 3 42.84301758998866 21.328446587273607 19.646059900851448 16.182475921886276 4 16.723829022000853 19.711532462398345 20.568896015088697 42.9957425005121 5 16.615288114685985 46.04284656503597 21.598372672021522 15.743492648256524 6 42.988949691802915 20.795049324121837 19.48265821339548 16.73334277067977 7 17.485938530287775 20.231775028852887 46.6220834176751 15.660203023184238 8 42.52684492944693 20.967286515713067 20.38014944443935 16.125719110400652 9 42.20535325542951 20.59574817674813 20.95759936635738 16.241299201464983 10-11 16.31200814879422 33.54703804899732 33.5587416448333 16.58221215737516 12-13 16.141991925261387 20.968718019460564 33.52365092253273 29.36563913274532 14-15 16.325015943455057 46.70632398690605 20.404232832741233 16.56442723689766 16-17 29.26667449779054 20.76959229064127 20.55005401929857 29.41367919226962 18-19 16.484021745508052 33.57825943757114 20.532638105287575 29.405080711633236 20-21 29.447545940788107 33.60725581249533 20.58121034488887 16.3639879018277 22-23 29.394247037049404 20.68070942484444 33.477024490891836 16.44801904721432 24-25 16.40753878281244 33.39586016073455 20.99517270964198 29.20142834681103 26-27 16.616811913486004 25.509796882557996 41.187844244343566 16.685546959612434 28-29 26.526081408190354 20.546431386222704 36.354129949282616 16.573357256304323 30-31 24.676769182318477 21.51803386177949 36.70176623968014 17.103430716221894 32-33 19.73375386584731 23.742214702165636 37.838290096901915 18.685741335085133 34-35 19.28628765336678 22.916442548107824 38.44544799269162 19.351821805833783 36-37 18.876221905561437 23.00361174689481 38.720276740357725 19.39988960718603 38-39 19.251377510285312 22.919025895990178 38.55913367940208 19.27046291432243 40-41 19.187911175359794 22.915264553561485 38.456265054375955 19.440559216702763 42-43 19.63268018296452 23.117256900905993 38.32137007046607 18.928692845663424 44-45 19.124279948949674 23.261105860439447 38.29199406712428 19.3226201234866 46-47 19.43555226972499 23.085609401380346 38.140578751615934 19.338259577278734 48-49 19.34417245461394 23.126203465317847 38.3377291378649 19.191894942203312 50-51 19.688006194510045 23.221581222643074 37.60961672296953 19.480795859877357 52-53 19.895304109970972 24.163835480630723 36.54272215163994 19.398138257758358 54-55 20.534284173590343 24.669879683769715 35.24452460461418 19.551311538025764 56-57 20.50715154495987 25.409128615225196 34.05451530870307 20.029204531111866 58-59 20.702755121013695 26.131222524827514 33.18524272967744 19.98077962448135 60-61 20.964536076284123 26.26544918644807 32.766855805238485 20.00315893202932 62-63 20.99721393705923 26.137414238421396 32.77946909106042 20.085902733458955 64-65 20.451918525321634 25.911616940550914 32.96200246910024 20.674462065027218 66-67 20.4374639903047 25.571364869548326 33.549568104405495 20.441603035741483 68-69 20.476808453244956 25.48762820238306 33.655181228314696 20.380382116057287 70-71 20.29953237596489 25.351085043509197 34.18650201337896 20.162880567146953 72-73 20.020930241798762 25.24277888446215 34.02021899105705 20.716071882682037 74-75 20.303797655575753 25.49032939623145 33.41315595936767 20.792716988825127 76-77 20.73875825863967 25.809719330557446 33.058503847202594 20.393018563600293 78-79 20.699807133856343 25.945642619758768 32.73670308332516 20.617847163059725 80-81 20.47220406490012 26.25256824007313 32.70448842379653 20.570739271230217 82-83 21.02009723058334 26.399361015488275 32.17978359443467 20.40075815949372 84-85 20.785191409729304 26.699086378494595 31.71851055571343 20.79721165606267 86-87 20.74843134645773 27.15398073932041 31.453666321483503 20.64392159273836 88-89 20.752740500331022 26.692871685841762 31.643189803972298 20.911198009854918 90-91 20.695960093359492 26.78223489835514 31.64035879156268 20.88144621672269 92-93 20.65435964483825 27.071517036355036 31.59239209042909 20.681731228377622 94-95 20.750399619874788 26.71194174283106 31.223069980179734 21.31458865711442 96-97 20.653759069679207 26.452548857617575 31.60083628135441 21.292855791348806 98-99 21.044270322628847 26.71855226510964 31.193580288387313 21.0435971238742 100-101 21.397751902372693 26.43092183126125 30.810514443157132 21.36081182320892 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15141.0 1 9183.5 2 2423.5 3 1424.5 4 1161.5 5 971.0 6 1003.0 7 1224.0 8 1137.0 9 1148.5 10 1588.0 11 9818.0 12 188812.0 13 210874.5 14 54647.0 15 48575.5 16 51368.0 17 54488.0 18 56574.5 19 57488.0 20 58729.5 21 60018.5 22 61588.0 23 64715.0 24 70089.5 25 77727.0 26 85927.5 27 94453.0 28 104392.5 29 115074.5 30 124541.5 31 134055.0 32 144723.5 33 156556.0 34 175074.0 35 189192.5 36 198194.5 37 210167.5 38 220279.0 39 230745.5 40 245034.0 41 257090.5 42 262992.5 43 272122.5 44 287437.0 45 296937.0 46 296272.0 47 294303.0 48 294770.5 49 294226.5 50 289565.0 51 284314.5 52 279430.5 53 273380.5 54 258025.5 55 237463.5 56 220778.0 57 206234.5 58 204935.5 59 177831.0 60 131426.0 61 102536.5 62 75862.5 63 55067.5 64 39128.0 65 26731.5 66 17385.5 67 10806.0 68 6631.5 69 4328.0 70 3031.0 71 2331.0 72 1990.5 73 1718.5 74 1533.0 75 1389.5 76 1280.0 77 1205.0 78 1133.5 79 1060.5 80 967.0 81 873.5 82 787.5 83 699.5 84 632.5 85 564.5 86 479.5 87 402.5 88 341.5 89 274.5 90 201.5 91 148.0 92 105.5 93 65.5 94 43.5 95 34.5 96 26.0 97 18.0 98 16.0 99 11.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12002240477062304 2 0.1091554070043703 3 0.10420182122056573 4 0.09218744500995736 5 0.0971520632788038 6 0.08741037898682291 7 0.0962253345352858 8 0.091944730339036 9 0.08750967135219985 10-11 0.07828099761466642 12-13 0.0704589657199728 14-15 0.07869471580373694 16-17 0.06914058375746802 18-19 0.06207427708814327 20-21 0.05794261143995884 22-23 0.05018125821299558 24-25 0.04740107198244158 26-27 0.04400306658954224 28-29 0.046661895484635556 30-31 0.037449770474664947 32-33 0.04982821869165539 34-35 0.06073934639807567 36-37 0.05157135132827259 38-39 0.05363994227362527 40-41 0.05403711173513298 42-43 0.049215915771831 44-45 0.04913317213401689 46-47 0.04815679720781043 48-49 0.048167829692852306 50-51 0.04971237759871564 52-53 0.046187498627834674 54-55 0.04660673305942615 56-57 0.04683841524530565 58-59 0.0494751791703152 60-61 0.045420740917423943 62-63 0.0545170248344548 64-65 0.050368810458707565 66-67 0.045023571455916234 68-69 0.04255229480653489 70-71 0.05096456465096914 72-73 0.04462640199440852 74-75 0.047020451248496686 76-77 0.042546778564013954 78-79 0.04667292796967744 80-81 0.0489290711607421 82-83 0.03877366867969066 84-85 0.048835295037886105 86-87 0.05184716345431961 88-89 0.047428653195046286 90-91 0.04296601299560543 92-93 0.04026305416034459 94-95 0.04378241688870462 96-97 0.03129364382129537 98-99 0.032258986262459946 100-101 0.023918427570797936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9064141.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.870713505953525 #Duplication Level Percentage of deduplicated Percentage of total 1 92.5536102326227 48.933754105506836 2 4.485532564311785 4.743066142587068 3 1.113844033421496 1.7666918634403093 4 0.4876051407050961 1.0312012679299716 5 0.2678683172011076 0.7081194528030821 6 0.1682541279014794 0.5337429475483908 7 0.11755453512057475 0.4350634503379822 8 0.09220221784619992 0.3899837635487961 9 0.07362711089424026 0.35034460977244014 >10 0.6108315552999649 6.065217559260359 >50 0.014054212027032588 0.4774297998161997 >100 0.008665058916549277 1.0784404955258997 >500 0.002924152509919702 1.1122166634842274 >1k 0.0029241525091349904 3.199247436035583 >5k 3.198291806866396E-4 1.2102923257694524 >10k+ 1.827595318209369E-4 27.965188116633417 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1098587 12.120144644704887 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 23725 0.261745707618626 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCACTCGGCCAGCTTC 22689 0.2503160531152373 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12663 0.1397043580853387 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9570 0.10558088185080086 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1032485041880969E-5 0.0 0.0 0.0 0.0 2 1.1032485041880969E-5 0.0 0.0 0.0 0.0 3 1.1032485041880969E-5 0.0 0.0 0.0 0.0 4 1.1032485041880969E-5 0.0 0.0 0.0 0.0 5 1.1032485041880969E-5 0.0 0.0 0.0 0.0 6 1.1032485041880969E-5 0.0 0.0 0.0 0.0 7 1.1032485041880969E-5 0.0 0.0 0.0 0.0 8 2.2064970083761937E-5 0.0 0.0 0.0 0.0 9 2.2064970083761937E-5 0.0 0.0 0.0 0.0 10-11 2.2064970083761937E-5 0.0 0.0 5.516242520940484E-6 0.0 12-13 2.7581212604702424E-5 0.0 0.0 1.1032485041880969E-5 0.0 14-15 4.4129940167523874E-5 0.0 0.0 1.1032485041880969E-5 0.0 16-17 4.964618268846436E-5 0.0 0.0 1.1032485041880969E-5 0.0 18-19 6.619491025128581E-5 0.0 0.0 1.1032485041880969E-5 0.0 20-21 6.619491025128581E-5 0.0 0.0 1.6548727562821454E-5 0.0 22-23 6.619491025128581E-5 0.0 0.0 2.2064970083761937E-5 0.0 24-25 6.619491025128581E-5 0.0 0.0 2.2064970083761937E-5 0.0 26-27 6.619491025128581E-5 0.0 0.0 2.2064970083761937E-5 1.1032485041880969E-5 28-29 6.619491025128581E-5 0.0 0.0 2.2064970083761937E-5 1.1032485041880969E-5 30-31 6.619491025128581E-5 0.0 0.0 2.2064970083761937E-5 1.1032485041880969E-5 32-33 6.619491025128581E-5 0.0 0.0 2.2064970083761937E-5 1.1032485041880969E-5 34-35 7.722739529316678E-5 0.0 0.0 2.2064970083761937E-5 1.1032485041880969E-5 36-37 7.722739529316678E-5 0.0 0.0 2.2064970083761937E-5 1.1032485041880969E-5 38-39 8.274363781410727E-5 0.0 0.0 2.2064970083761937E-5 1.1032485041880969E-5 40-41 9.377612285598823E-5 0.0 0.0 2.7581212604702424E-5 1.1032485041880969E-5 42-43 1.1032485041880968E-4 0.0 0.0 3.309745512564291E-5 1.1032485041880969E-5 44-45 1.434223055444526E-4 0.0 0.0 3.309745512564291E-5 1.1032485041880969E-5 46-47 1.5445479058633356E-4 0.0 0.0 4.4129940167523874E-5 1.1032485041880969E-5 48-49 1.7100351814915501E-4 0.0 0.0 5.516242520940484E-5 1.1032485041880969E-5 50-51 1.8755224571197646E-4 0.0 0.0 5.516242520940484E-5 2.2064970083761937E-5 52-53 2.1513345831667888E-4 0.0 0.0 5.516242520940484E-5 2.2064970083761937E-5 54-55 2.482309134423218E-4 0.0 0.0 6.067866773034533E-5 2.2064970083761937E-5 56-57 2.9787709613078616E-4 0.0 0.0 6.619491025128581E-5 2.2064970083761937E-5 58-59 3.6958824890301247E-4 0.0 0.0 6.619491025128581E-5 2.2064970083761937E-5 60-61 4.3578315915429827E-4 0.0 0.0 6.619491025128581E-5 2.7581212604702424E-5 62-63 5.13010554447465E-4 0.0 0.0 6.619491025128581E-5 3.309745512564291E-5 64-65 5.736892221778103E-4 0.0 0.0 6.619491025128581E-5 3.309745512564291E-5 66-67 6.564328599919176E-4 0.0 0.0 6.619491025128581E-5 3.309745512564291E-5 68-69 7.226277702432034E-4 0.0 0.0 6.619491025128581E-5 3.309745512564291E-5 70-71 8.495013482248345E-4 0.0 0.0 6.619491025128581E-5 3.309745512564291E-5 72-73 9.543099561227038E-4 0.0 0.0 6.619491025128581E-5 3.309745512564291E-5 74-75 0.0010977322616671564 5.516242520940484E-6 0.0 7.17111527722263E-5 3.309745512564291E-5 76-77 0.0012687357798163114 1.1032485041880969E-5 0.0 7.722739529316678E-5 3.309745512564291E-5 78-79 0.001550064148384276 1.1032485041880969E-5 0.0 7.722739529316678E-5 3.309745512564291E-5 80-81 0.0018148437893894193 1.1032485041880969E-5 0.0 7.722739529316678E-5 4.4129940167523874E-5 82-83 0.0022009807658552533 1.1032485041880969E-5 0.0 8.825988033504775E-5 4.4129940167523874E-5 84-85 0.002835348655763409 2.2064970083761937E-5 0.0 8.825988033504775E-5 4.4129940167523874E-5 86-87 0.0038337885520536366 2.2064970083761937E-5 0.0 9.377612285598823E-5 4.4129940167523874E-5 88-89 0.0050970080893490075 2.2064970083761937E-5 0.0 9.929236537692872E-5 5.516242520940484E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 236460 0.0 91.30408 5 AAGCAGT 238425 0.0 91.12126 1 GGTATCA 237915 0.0 91.040184 8 GTATCAA 238405 0.0 90.98757 9 TGGTATC 237705 0.0 90.92527 7 GTGGTAT 238690 0.0 90.64465 6 GCAGTGG 240275 0.0 90.08186 3 AGCAGTG 240805 0.0 90.070145 2 CAGTGGT 240675 0.0 89.70651 4 TCAACGC 237360 0.0 46.261612 12-13 CAACGCA 237030 0.0 46.126583 12-13 AACGCAG 238225 0.0 46.11131 14-15 ATCAACG 237160 0.0 46.11073 10-11 CGCAGAG 238430 0.0 46.0397 16-17 ACGCAGA 238155 0.0 45.96228 14-15 TATCAAC 238855 0.0 45.889935 10-11 CAGAGTA 237745 0.0 45.641846 18-19 AGAGTAC 233335 0.0 45.57002 18-19 GAGTACT 141210 0.0 45.31351 20-21 GCAGAGT 240785 0.0 45.28361 16-17 >>END_MODULE