FastQCFastQC Report
Sat 18 Jun 2016
SRR578588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9064141
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1483471.6366360585079158No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291840.32197204346225416No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100000.11032485041880968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4200.040.69343
CTCGCGT3450.025.46110752-53
CGCGTAG3650.024.06392154-55
ACTCGCG3650.023.41664550-51
CGCTAGT3850.022.19725890-91
CGTACGC4000.021.36415320-21
CAACGCA61250.020.73379912-13
ATCAACG61700.020.54416310-11
CGTAGCG4300.020.42556456-57
TCGCGTA4200.020.34922652-53
ACGTACG4550.018.78167320-21
TCAACGC60450.017.19927810-11
AACGCAG64550.016.36420612-13
ACGCAGA79850.016.17168214-15
AGTGGTA82500.015.8826695
ACGCTAG5850.015.82582390-91
GGTATCA84200.015.7873618
GTATCAA88100.015.3579259
AAGCAGT93750.015.0136591
TGGTATC92150.014.6315047
Produced by FastQC (version 0.11.5)