FastQCFastQC Report
Sat 18 Jun 2016
SRR578587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8224518
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95671211.63243852101728No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT203100.24694456258713277No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG94360.11473012765003372No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA91690.11148373679770657No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA84160.10232818506810976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2119550.091.371825
AAGCAGT2135100.091.0812451
GGTATCA2130900.091.076128
TGGTATC2131050.091.009577
GTATCAA2132250.091.0073249
GTGGTAT2143300.090.538146
GCAGTGG2156250.090.052373
AGCAGTG2165850.089.816612
CAGTGGT2159600.089.678984
TCAACGC2122700.046.3076912-13
AACGCAG2130950.046.16708414-15
CAACGCA2122100.046.13065312-13
ATCAACG2121350.046.12346310-11
CGCAGAG2137300.046.00045816-17
ACGCAGA2133900.045.8863914-15
TATCAAC2139350.045.87517510-11
CAGAGTA2127950.045.59329218-19
AGAGTAC2091450.045.4699718-19
GAGTACT1230450.045.2262420-21
GCAGAGT2156750.045.2170316-17
Produced by FastQC (version 0.11.5)