##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578587_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8224518
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07950192339539	31.0	31.0	34.0	28.0	34.0
2	31.4370607493351	33.0	31.0	34.0	28.0	34.0
3	31.553435715016978	34.0	31.0	34.0	28.0	34.0
4	34.94525101653373	37.0	35.0	37.0	32.0	37.0
5	34.980606644669024	37.0	35.0	37.0	32.0	37.0
6	35.01707638064626	37.0	35.0	37.0	32.0	37.0
7	34.95329999885708	37.0	35.0	37.0	32.0	37.0
8	34.991958191349326	37.0	35.0	37.0	32.0	37.0
9	36.50018493484968	39.0	37.0	39.0	32.0	39.0
10-11	36.38632336873723	39.0	37.0	39.0	32.0	39.0
12-13	36.437887484713386	39.0	37.0	39.0	32.0	39.0
14-15	37.69686466732762	40.0	38.0	41.0	32.0	41.0
16-17	37.672935666260315	40.0	38.0	41.0	32.0	41.0
18-19	37.606991011509734	40.0	38.0	41.0	32.0	41.0
20-21	37.539530827216865	40.0	38.0	41.0	32.0	41.0
22-23	37.26990134862614	39.5	37.5	41.0	31.5	41.0
24-25	37.22574459682622	40.0	37.0	41.0	31.0	41.0
26-27	37.114296485216514	40.0	37.0	41.0	31.0	41.0
28-29	36.949909101056136	40.0	37.0	41.0	30.0	41.0
30-31	36.72633302036667	40.0	36.0	41.0	30.0	41.0
32-33	36.44552434562122	39.5	36.0	41.0	30.0	41.0
34-35	36.26244607890699	39.0	36.0	41.0	29.0	41.0
36-37	35.97887456748225	39.0	35.0	40.5	27.5	41.0
38-39	35.139839015490026	38.0	35.0	40.0	27.0	41.0
40-41	34.913147068314515	38.0	34.5	40.0	26.0	41.0
42-43	34.61599536896873	37.5	34.0	40.0	25.0	41.0
44-45	34.32326921772194	37.0	33.5	40.0	24.0	41.0
46-47	34.19133905476285	37.0	33.5	40.0	24.0	41.0
48-49	34.22872610893428	38.0	33.5	40.0	23.5	41.0
50-51	33.384646553147554	37.0	32.5	39.5	21.5	40.5
52-53	33.02360083593956	37.5	33.0	39.5	14.5	40.5
54-55	32.711240950533515	38.0	32.5	40.0	4.5	41.0
56-57	32.24165008818754	38.0	32.0	40.0	2.0	41.0
58-59	31.957320842388576	37.5	31.5	40.0	2.0	41.0
60-61	31.69266448197937	37.0	31.0	40.0	2.0	41.0
62-63	31.35523534631452	36.5	31.0	40.0	2.0	41.0
64-65	31.05893773957331	36.0	31.0	39.5	2.0	41.0
66-67	30.72042507536612	35.5	30.5	39.0	2.0	41.0
68-69	30.38449791221808	35.0	30.0	39.0	2.0	41.0
70-71	30.081453952681482	35.0	30.0	38.5	2.0	40.5
72-73	29.755754501358012	35.0	29.0	37.5	2.0	40.0
74-75	29.388624293849197	35.0	29.0	37.0	2.0	39.0
76-77	29.014411604424723	35.0	29.0	36.5	2.0	39.0
78-79	28.667952152332816	35.0	29.0	36.0	2.0	39.0
80-81	28.335717485207034	35.0	28.0	36.0	2.0	37.5
82-83	28.063069714237358	34.5	27.5	35.0	2.0	37.0
84-85	27.790369052630197	34.0	27.0	35.0	2.0	37.0
86-87	27.546836240129814	34.0	26.5	35.0	2.0	36.0
88-89	27.289509731755707	34.0	26.0	35.0	2.0	36.0
90-91	27.055321904578484	34.0	26.0	35.0	2.0	36.0
92-93	26.778565382190177	34.0	25.0	35.0	2.0	35.0
94-95	26.48838381775078	34.0	25.0	35.0	2.0	35.0
96-97	26.211902326677382	34.0	24.0	35.0	2.0	35.0
98-99	25.899694608243305	34.0	21.5	35.0	2.0	35.0
100-101	25.04050991194864	33.0	10.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6085152301208296
1101	2	0.6076528855537262
1101	3	0.5193182399838676
1101	4	0.5924493667591335
1101	5	0.5386353518378968
1101	6	0.5327972352316266
1101	7	0.501476796995064
1101	8	0.5167574435255773
1101	9	0.6237109700423531
1101	10-11	0.6886921313532497
1101	12-13	0.7403072597962677
1101	14-15	0.8877620254358618
1101	16-17	0.8404245056220603
1101	18-19	0.8034199290914898
1101	20-21	0.8791328194771069
1101	22-23	0.8859235394893048
1101	24-25	0.8434998824223428
1101	26-27	0.9263834658418659
1101	28-29	1.0377555029512848
1101	30-31	1.109292274719472
1101	32-33	1.0837989885519619
1101	34-35	1.159746422857836
1101	36-37	1.1099259963397472
1101	38-39	1.1582503264260495
1101	40-41	1.1219808039396995
1101	42-43	1.1555692317969388
1101	44-45	1.2721062015298656
1101	46-47	1.1913006798951642
1101	48-49	1.1452685818729407
1101	50-51	1.2440853037324473
1101	52-53	1.0538435154364478
1101	54-55	1.08181508812077
1101	56-57	1.0489851571943873
1101	58-59	1.095336138674007
1101	60-61	1.0108008802076753
1101	62-63	0.969988211848893
1101	64-65	1.010337582566848
1101	66-67	1.0249749916041182
1101	68-69	0.9170543815141166
1101	70-71	1.061177848823256
1101	72-73	1.0112753265214742
1101	74-75	0.9824884618118688
1101	76-77	0.8895317118303545
1101	78-79	0.9951108388336998
1101	80-81	0.9524575134636422
1101	82-83	0.9760510233110828
1101	84-85	0.9748877332449304
1101	86-87	0.9946113958137239
1101	88-89	0.9763347934461493
1101	90-91	1.0268056760534279
1101	92-93	1.1363959940536255
1101	94-95	1.2191654651259292
1101	96-97	1.1800841959121264
1101	98-99	1.1449580318248422
1101	100-101	1.275508402187711
1102	1	0.5292842559806736
1102	2	0.506527726243732
1102	3	0.5503451433641118
1102	4	0.5291610862317953
1102	5	0.5670349324692268
1102	6	0.5756803337698244
1102	7	0.5935070936935176
1102	8	0.5253354513532997
1102	9	0.6659777359302765
1102	10-11	0.7004934982116424
1102	12-13	0.6807469370583448
1102	14-15	0.865588200053061
1102	16-17	0.8652312196754295
1102	18-19	0.8731650880837805
1102	20-21	0.8826466695185928
1102	22-23	0.8913988850835537
1102	24-25	0.9092682301073225
1102	26-27	0.9678276606039091
1102	28-29	1.0252582370346346
1102	30-31	1.062537469758901
1102	32-33	1.138065178907702
1102	34-35	1.079076129483056
1102	36-37	1.108283664542391
1102	38-39	1.1006379968403976
1102	40-41	1.179365779983634
1102	42-43	1.1735573680135474
1102	44-45	1.248053862315352
1102	46-47	1.2747365948141507
1102	48-49	1.1683388383452282
1102	50-51	1.150550448599148
1102	52-53	0.958118405981466
1102	54-55	0.9067118650183446
1102	56-57	0.8851174342856467
1102	58-59	0.9033098926957024
1102	60-61	0.8835861321649077
1102	62-63	0.9868705442327794
1102	64-65	0.9486464099597356
1102	66-67	0.8756310921841717
1102	68-69	0.8231130308097327
1102	70-71	0.8363012949780071
1102	72-73	0.8066247148810568
1102	74-75	0.7583072754824087
1102	76-77	0.8436581492861386
1102	78-79	0.8260547116154271
1102	80-81	0.8405911823407912
1102	82-83	0.8896611436351876
1102	84-85	0.9050294840113935
1102	86-87	0.916300546115604
1102	88-89	0.9625243427103598
1102	90-91	0.9680940066801753
1102	92-93	0.8725267559433796
1102	94-95	0.9964642946327693
1102	96-97	1.0848286095230364
1102	98-99	1.1025771981463102
1102	100-101	1.1824094729708392
1103	1	0.4474476454225922
1103	2	0.4755775455187248
1103	3	0.3655945906614271
1103	4	0.4676376933586397
1103	5	0.4803619881917953
1103	6	0.5273086914142766
1103	7	0.42182266645725974
1103	8	0.42973410693082315
1103	9	0.549066320426526
1103	10-11	0.564476661737551
1103	12-13	0.6002809901723936
1103	14-15	0.6596985144404854
1103	16-17	0.801621457015905
1103	18-19	0.7485184668810589
1103	20-21	0.7586162484507781
1103	22-23	0.8196948256918972
1103	24-25	0.7577442133722272
1103	26-27	0.8073748928014197
1103	28-29	0.8301246525968793
1103	30-31	0.8875747260140869
1103	32-33	0.8999149880449409
1103	34-35	0.9015022505824533
1103	36-37	1.0065010180420586
1103	38-39	0.8203714323311218
1103	40-41	0.9211176038050226
1103	42-43	0.8501814755985464
1103	44-45	0.7466732363478741
1103	46-47	0.9906943983522325
1103	48-49	1.0896736494515338
1103	50-51	1.0422928560043943
1103	52-53	0.8999768322117419
1103	54-55	0.7418339275059225
1103	56-57	0.7489741098033491
1103	58-59	0.7177702521181644
1103	60-61	0.7711879873743541
1103	62-63	0.6327251571069539
1103	64-65	0.574478817248
1103	66-67	0.5870020872829542
1103	68-69	0.7502731917661158
1103	70-71	0.6899499125389426
1103	72-73	0.7947171985144301
1103	74-75	0.7969684677605571
1103	76-77	0.7949521364267831
1103	78-79	0.7803183400744444
1103	80-81	0.8021514025640535
1103	82-83	0.8022317862519799
1103	84-85	0.7997151355767009
1103	86-87	0.8279642884294276
1103	88-89	0.8623826090760112
1103	90-91	0.8192978847619514
1103	92-93	0.825266055736332
1103	94-95	0.7523758739002773
1103	96-97	0.7372552375102615
1103	98-99	0.7843043599254074
1103	100-101	0.9170544760316588
1104	1	0.3822535541915748
1104	2	0.36506035417350446
1104	3	0.41918999038659877
1104	4	0.36863987305526535
1104	5	0.44478089371725815
1104	6	0.4792260193541651
1104	7	0.40941081944998103
1104	8	0.358575046217382
1104	9	0.5499549139617557
1104	10-11	0.5175201897988444
1104	12-13	0.5695935856564915
1104	14-15	0.6865459372740261
1104	16-17	0.640868321656292
1104	18-19	0.6929322721192577
1104	20-21	0.7313150902716927
1104	22-23	0.7082693314408814
1104	24-25	0.6487331244456627
1104	26-27	0.7146976075704998
1104	28-29	0.7286314142113142
1104	30-31	0.7957374073444754
1104	32-33	0.7870386444872466
1104	34-35	0.8744303972763419
1104	36-37	0.8466303683561875
1104	38-39	0.9250272890736895
1104	40-41	0.8632583687611728
1104	42-43	1.0253769763932183
1104	44-45	0.9593530527746879
1104	46-47	1.0965009479636905
1104	48-49	0.9812836211818663
1104	50-51	1.0792641725728842
1104	52-53	0.9924637669052885
1104	54-55	0.9384010344203588
1104	56-57	0.882176049190555
1104	58-59	0.9055497996318778
1104	60-61	0.973647545919377
1104	62-63	0.8585435390281901
1104	64-65	0.732456271362782
1104	66-67	0.8711333021295466
1104	68-69	0.8321376302323991
1104	70-71	0.7348638788144086
1104	72-73	0.7976378430156785
1104	74-75	0.8506615579747461
1104	76-77	0.8425886559547955
1104	78-79	0.7398215651107662
1104	80-81	0.885817359235169
1104	82-83	0.8890843171747917
1104	84-85	0.8728878307905852
1104	86-87	0.8841505935859892
1104	88-89	0.9026934119176246
1104	90-91	0.8935715071484687
1104	92-93	0.7582283358649313
1104	94-95	0.640881172080018
1104	96-97	0.7509461725112843
1104	98-99	0.9980098382499136
1104	100-101	0.9416078358737572
1105	1	0.2584145668341655
1105	2	0.23356163367634153
1105	3	0.22681801752681707
1105	4	0.2500061664609987
1105	5	0.16478645855935525
1105	6	0.2025663809924012
1105	7	0.17258088671967897
1105	8	0.17931119492446612
1105	9	0.12653814908157557
1105	10-11	0.1862066618065441
1105	12-13	0.1845175628015383
1105	14-15	0.07957870679874901
1105	16-17	0.10337271877408227
1105	18-19	0.012010443558615691
1105	20-21	0.10747369450501765
1105	22-23	0.062462818223892214
1105	24-25	0.03475366874332764
1105	26-27	-0.29728352013455606
1105	28-29	-0.11750799783365551
1105	30-31	-0.20298153143674114
1105	32-33	-0.20960176626837068
1105	34-35	-0.13005781504787706
1105	36-37	-0.0446833636425481
1105	38-39	-0.029418145651980865
1105	40-41	0.04104969029925343
1105	42-43	0.13650678809386108
1105	44-45	0.10849514197905563
1105	46-47	0.13702555050178233
1105	48-49	0.18052664398483387
1105	50-51	0.15054359465666067
1105	52-53	-0.025128671029655436
1105	54-55	-0.011933741550116395
1105	56-57	0.11073090021556453
1105	58-59	0.11473735676962349
1105	60-61	0.068716172124482
1105	62-63	0.08353557356874575
1105	64-65	-0.0019127629543582714
1105	66-67	0.011154037116551052
1105	68-69	0.07698359900156504
1105	70-71	0.07158811071248294
1105	72-73	0.044480241388953345
1105	74-75	0.10674821027312831
1105	76-77	0.16742724649136065
1105	78-79	0.1899118167059477
1105	80-81	0.23721942885208236
1105	82-83	0.2089263085269053
1105	84-85	0.14791244260814196
1105	86-87	0.13578027204493637
1105	88-89	0.2596642904245705
1105	90-91	0.29087339459047
1105	92-93	0.3312949392896023
1105	94-95	0.318214329174463
1105	96-97	0.3605418275143073
1105	98-99	0.4328579051685537
1105	100-101	0.4365644852154631
1106	1	-0.11800495866265237
1106	2	-0.05919682898737122
1106	3	-0.11959687659433982
1106	4	-0.12229844645379728
1106	5	-0.15320920862752985
1106	6	-0.15988657248463767
1106	7	-0.17996728911865745
1106	8	-0.20611424497353426
1106	9	-0.10510384212179247
1106	10-11	-0.32639649593998854
1106	12-13	-0.3334904779013499
1106	14-15	-0.31293202923585994
1106	16-17	-0.35597087689767903
1106	18-19	-0.35214378591523143
1106	20-21	-0.19142695002502563
1106	22-23	-0.1448027952173092
1106	24-25	-0.2570310686024371
1106	26-27	-0.2684837870881438
1106	28-29	-0.2907625652709953
1106	30-31	-0.2689473167756731
1106	32-33	-0.22000433033301903
1106	34-35	-0.21064931585114977
1106	36-37	-0.29537287077754115
1106	38-39	-0.29282264898798616
1106	40-41	-0.3381610849930681
1106	42-43	-0.4150041750099831
1106	44-45	-0.3400458446495591
1106	46-47	-0.4090099786486405
1106	48-49	-0.6399079514763457
1106	50-51	-0.762834810841241
1106	52-53	-0.7983226976641546
1106	54-55	-0.7247188986304991
1106	56-57	-0.7298062439986168
1106	58-59	-0.6579635149088183
1106	60-61	-0.8592091667339758
1106	62-63	-0.7168972488357213
1106	64-65	-0.6754869677759743
1106	66-67	-0.8278230885766504
1106	68-69	-0.9738386178852814
1106	70-71	-0.8950450322160819
1106	72-73	-0.8864092570447184
1106	74-75	-0.9460639838490295
1106	76-77	-0.9844499447934432
1106	78-79	-0.888340198146004
1106	80-81	-0.8652105785421575
1106	82-83	-0.9695081362960707
1106	84-85	-1.0160071828916735
1106	86-87	-0.9573075423108968
1106	88-89	-1.002170940873814
1106	90-91	-0.9079961743314371
1106	92-93	-0.9032002352511803
1106	94-95	-0.917081486576599
1106	96-97	-0.9241616373726522
1106	98-99	-0.9255515913443055
1106	100-101	-1.079299288583286
1107	1	-0.3675894139669573
1107	2	-0.4227391224425965
1107	3	-0.42128098934205127
1107	4	-0.316122108959199
1107	5	-0.366254862629809
1107	6	-0.3173312642190069
1107	7	-0.29512462574122367
1107	8	-0.2553270757902766
1107	9	-0.3582617080824022
1107	10-11	-0.3354175020419916
1107	12-13	-0.4329587092131675
1107	14-15	-0.663045770891614
1107	16-17	-0.5170238574116581
1107	18-19	-0.45345665010901826
1107	20-21	-0.34660765344830224
1107	22-23	-0.36491704109404566
1107	24-25	-0.4122639563122732
1107	26-27	-0.31685933882507555
1107	28-29	-0.398543342548777
1107	30-31	-0.39272917140873886
1107	32-33	-0.3339597219783812
1107	34-35	-0.3669302683316289
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2108	2	-1.1401094523421769
2108	3	-1.086914825539985
2108	4	-1.1609738850241698
2108	5	-1.4201263779372937
2108	6	-1.1815326491958515
2108	7	-1.0874226531776543
2108	8	-0.9749467892619137
2108	9	-1.2719303207812587
2108	10-11	-1.7910596718425609
2108	12-13	-1.3013498981043057
2108	14-15	-1.3993463668535853
2108	16-17	-1.4520745464074665
2108	18-19	-1.3533769298656324
2108	20-21	-1.3249777098034912
2108	22-23	-1.30820142655962
2108	24-25	-1.3963713482336502
2108	26-27	-1.5285585272064282
2108	28-29	-1.8035581945312913
2108	30-31	-2.002192852848232
2108	32-33	-1.9927080194372664
2108	34-35	-2.6971699044214006
2108	36-37	-2.5324067396871968
2108	38-39	-2.2931538983845527
2108	40-41	-2.2001791132246993
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2108	54-55	-2.066069729903681
2108	56-57	-1.8480788581526468
2108	58-59	-1.7211056138612904
2108	60-61	-1.5903571716974483
2108	62-63	-1.4722838371589404
2108	64-65	-1.6205548119825472
2108	66-67	-1.4786838230718047
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2108	74-75	-1.5138969072410688
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2108	78-79	-1.3919120304818904
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2201	28-29	1.0518880465546374
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2201	34-35	1.1910861153437793
2201	36-37	1.184664352764777
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2201	54-55	1.2799911423148131
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2202	32-33	1.1595669882172004
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2202	40-41	1.2960925371569871
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2202	48-49	1.373140102801166
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2202	66-67	1.357422061298152
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2207	64-65	-0.5194311800448617
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2207	88-89	-0.4537548827553799
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2208	2	-0.6748699095921857
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2208	5	-0.5960373793127118
2208	6	-0.6628424949988201
2208	7	-0.5634221207335415
2208	8	-0.5838175159287573
2208	9	-0.7612526327089171
2208	10-11	-0.901311827859594
2208	12-13	-0.8326523882245596
2208	14-15	-1.227739121343994
2208	16-17	-1.0268538472322462
2208	18-19	-1.2219741818043843
2208	20-21	-1.1393799170420067
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2308	40-41	-2.5712389623503
2308	42-43	-2.5966900258902825
2308	44-45	-2.7097440179287204
2308	46-47	-2.788452756809484
2308	48-49	-2.5094430136891894
2308	50-51	-2.4542782763718947
2308	52-53	-2.3632771722488
2308	54-55	-2.014632216288021
2308	56-57	-1.998219840575974
2308	58-59	-1.81486880369674
2308	60-61	-1.799099918554795
2308	62-63	-1.8676343050566828
2308	64-65	-1.7699542466928335
2308	66-67	-1.9879541713387496
2308	68-69	-1.8248526416039041
2308	70-71	-1.7859644083831796
2308	72-73	-1.7369465163979427
2308	74-75	-1.5599386637858004
2308	76-77	-1.513616344388769
2308	78-79	-1.5995548492620344
2308	80-81	-2.229694790153186
2308	82-83	-2.036355196066083
2308	84-85	-1.771277913864214
2308	86-87	-1.6086803309198032
2308	88-89	-1.5705989101895845
2308	90-91	-1.777134914460028
2308	92-93	-1.8144488418634772
2308	94-95	-1.9464691046279192
2308	96-97	-1.8485316391057474
2308	98-99	-1.8658959082316073
2308	100-101	-2.03356404923327
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66025.0
3	12114.0
4	10574.0
5	10254.0
6	12209.0
7	14885.0
8	18118.0
9	21041.0
10	24200.0
11	29290.0
12	29043.0
13	31845.0
14	35053.0
15	41452.0
16	52212.0
17	71256.0
18	109368.0
19	175098.0
20	250964.0
21	183256.0
22	102589.0
23	86603.0
24	89054.0
25	94115.0
26	100010.0
27	111766.0
28	128309.0
29	150215.0
30	177096.0
31	212010.0
32	261011.0
33	330013.0
34	436026.0
35	639923.0
36	1049562.0
37	1409874.0
38	1365272.0
39	282483.0
40	330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.744769436734773	46.28252736096893	25.50708346943108	17.465619732865218
2	16.945475292288112	48.21075875336977	18.731327144473518	16.1124388098686
3	42.42948469731592	21.444238192810584	19.785111866294425	16.34116524357907
4	16.85398603076259	19.81327309916314	20.697977680801962	42.6347631892723
5	16.73277804320223	45.60059171669641	21.766093747376008	15.900536492725356
6	42.51302339810136	20.958223477669208	19.62934078819828	16.89941233603115
7	17.621764728626655	20.320019901692834	46.32195608544596	15.736259284234547
8	42.079317715894916	21.084575408492558	20.588012849975804	16.24809402563672
9	41.75406903933207	20.692222170745282	21.13188793380925	16.4218208561134
10-11	16.40706001047818	33.39552106090703	33.47440229710137	16.723016631513424
12-13	16.24939104785008	21.06184594647642	33.42677896113189	29.261984044541613
14-15	16.425041732065395	46.2949374011419	20.555554035538602	16.724466831254105
16-17	29.0967841795671	20.876162181761057	20.70174220569471	29.325311432977134
18-19	16.602431524462975	33.43371670150174	20.678426694972444	29.285425079062843
20-21	29.26965861796022	33.473988116009345	20.71553198913978	16.54082127689066
22-23	29.191469886904187	20.775880658324382	33.438142308337426	16.594507146434008
24-25	16.52560152647271	33.26115627657092	21.104363075419865	29.108879121536503
26-27	16.727896016313885	25.758001596589565	40.642822146840885	16.87128024025566
28-29	26.876811682311168	20.670121832595694	35.72802271290307	16.72504377219007
30-31	25.063997689723834	21.648813703585486	36.064134482391744	17.223054124298937
32-33	19.93354855610398	23.918756656558454	37.228894663099034	18.918800124238533
34-35	19.46866764141738	23.07020940928283	37.88197207673578	19.57915087256401
36-37	19.055550073952997	23.17959170795043	38.10262544850097	19.6622327695956
38-39	19.465163675423003	23.077569846128743	37.93210993189034	19.525156546557916
40-41	19.480277007747762	23.051424832657922	37.775752465590614	19.692545694003698
42-43	19.90390687827394	23.271640619433498	37.618096502390806	19.206355999901756
44-45	19.406621657868122	23.45175358497439	37.54322773052986	19.598397026627627
46-47	19.751963373026253	23.234678680791333	37.42683459967228	19.58652334651013
48-49	19.572151887806417	23.299450696261168	37.68145344043924	19.446943975493173
50-51	19.93871716420973	23.399957138492823	36.89665665044921	19.764669046848233
52-53	20.22362727699561	24.430198289905476	35.697095476351166	19.649078956747744
54-55	20.913501183283646	24.99707017593738	34.23559535638258	19.853833284396394
56-57	20.901491283190413	25.759192987716673	33.01005387017118	20.32926185892174
58-59	21.037446488582162	26.50027353729371	32.19416473250926	20.268115241614872
60-61	21.264367221485852	26.614207242679626	31.83888932538806	20.282536210446455
62-63	21.31430900897929	26.430570414462785	31.901009064011177	20.354111512546748
64-65	20.705780144386445	26.205664076741225	32.11452973370662	20.97402604516571
66-67	20.72024122636958	25.80192806279833	32.79661261456919	20.6812180962629
68-69	20.769504668436113	25.746988435155117	32.83892770704187	20.6445791893669
70-71	20.583270349085815	25.611972844369834	33.37978250393473	20.42497430260962
72-73	20.283498487833587	25.531723236723884	33.1973874730905	20.987390802352028
74-75	20.59535299656204	25.769314180699244	32.56338359766638	21.07194922507234
76-77	21.039889647246174	26.13424466072372	32.17789633366855	20.64796935836156
78-79	21.00573885305374	26.235540462898793	31.850540080251765	20.9081806037957
80-81	20.7910084999201	26.559996347393334	31.816620882256704	20.832374270429867
82-83	21.348463689871025	26.714289261799866	31.303475297893808	20.6337717504353
84-85	21.09603135768484	27.047451828986258	30.796610558427133	21.05990625490177
86-87	21.050294540666723	27.493765369730877	30.56795806340341	20.88798202619899
88-89	21.032906404756435	27.015554876287368	30.769035060585733	21.182503658370464
90-91	20.985702780735185	27.10356976978476	30.800862203830487	21.109865245649566
92-93	20.965147635895082	27.42797870729619	30.71585705884742	20.89101659796131
94-95	21.052072046784197	27.08234148699774	30.304737207275306	21.560849258942753
96-97	20.96257159229272	26.8122806138395	30.688242573194884	21.536905220672896
98-99	21.391486782521117	27.05827569345618	30.31726040366908	21.23297712035362
100-101	21.516298254983013	26.911899618349626	30.063374645868425	21.508427480798932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11477.0
1	6778.5
2	1536.0
3	854.5
4	705.5
5	658.0
6	762.5
7	896.5
8	852.5
9	954.5
10	1475.5
11	7720.0
12	145474.5
13	162164.5
14	42602.5
15	40094.5
16	43681.5
17	46852.0
18	49242.0
19	50724.5
20	52076.5
21	53526.5
22	55279.5
23	58416.0
24	63230.0
25	69740.0
26	76991.0
27	85125.5
28	94073.0
29	103917.5
30	112728.5
31	120813.5
32	130506.5
33	141138.0
34	158220.0
35	171508.5
36	179635.0
37	190509.5
38	199690.5
39	209596.5
40	223028.0
41	233568.0
42	239163.0
43	248502.5
44	262510.0
45	271382.0
46	271642.0
47	270985.5
48	272574.5
49	271947.0
50	267511.0
51	263847.5
52	260384.0
53	256097.5
54	242232.0
55	223328.5
56	208238.5
57	195260.5
58	196723.0
59	169675.0
60	123064.0
61	95450.5
62	69583.0
63	49949.0
64	35278.5
65	23409.5
66	14381.5
67	8741.0
68	5103.0
69	2957.0
70	1970.0
71	1404.5
72	1162.0
73	1018.0
74	878.0
75	759.5
76	674.5
77	596.0
78	501.5
79	434.0
80	376.0
81	330.5
82	262.0
83	232.5
84	223.5
85	177.5
86	140.0
87	101.5
88	75.0
89	58.5
90	36.5
91	26.5
92	23.5
93	18.5
94	11.0
95	5.5
96	3.5
97	4.5
98	4.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052562350766330625
2	0.09375625416589763
3	0.08438184462603159
4	0.0559181705237924
5	0.0859868019013394
6	0.001094289051346231
7	0.0011186065858205915
8	0.00459601401565417
9	0.009374409539866044
10-11	0.009271060018350012
12-13	0.01673046371836015
14-15	0.011252939078010407
16-17	0.0014104169995129198
18-19	5.836208273846564E-4
20-21	9.727013789744275E-5
22-23	3.830011679711808E-4
24-25	8.146374048910829E-4
26-27	0.0013678613141827887
28-29	0.0014347345339872804
30-31	0.004778395524211875
32-33	2.0061965941347567E-4
34-35	0.002346642076775806
36-37	4.5595377139426285E-4
38-39	0.0025229442017149213
40-41	0.0033011053048944633
42-43	0.0012158767237180344
44-45	6.322558963333778E-4
46-47	0.00106389213325328
48-49	0.002401356529343118
50-51	0.004322441752817612
52-53	0.13084657362291627
54-55	0.14064654001608362
56-57	0.12666395769332622
58-59	0.12056633592388027
60-61	0.10819479026004929
62-63	0.11284551872827076
64-65	0.11527727217570682
66-67	0.12352091636251511
68-69	0.13643960655201923
70-71	0.12356347204784524
72-73	0.1153015897101812
74-75	0.12052378023855015
76-77	0.13201989466130418
78-79	0.14576538102293654
80-81	0.13624506627622435
82-83	0.11562987642558507
84-85	0.12169102189331946
86-87	0.1230163275221721
88-89	0.11909512508818147
90-91	0.12989211039479762
92-93	0.14631252554860966
94-95	0.15527353700241156
96-97	0.15596658673493086
98-99	0.13926651993466363
100-101	0.1287187893564097
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8224518.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.379365465286845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.4403490170006	51.192878719511455
2	4.690198173969362	5.194803975617406
3	1.1049837958880995	1.8357990439712097
4	0.4701729323398832	1.041515146077439
5	0.25455799242250676	0.704863004723786
6	0.16137429418244156	0.5362083608539286
7	0.11130578437753713	0.43148305980108304
8	0.08722071341602122	0.3864182211527109
9	0.07348485089653801	0.36625899725653416
>10	0.5803769858658279	5.894883964565258
>50	0.011348334911247863	0.4016288538076217
>100	0.009098666363636109	1.1661290833042517
>500	0.0025515961692526745	0.9699071511215881
>1k	0.002598847949798592	3.0455843406319136
>5k	2.8351068543257366E-4	1.2651838889670872
>10k+	9.450356181085789E-5	25.56645418863682
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	956712	11.63243852101728	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	20310	0.24694456258713277	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	9436	0.11473012765003372	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA	9169	0.11148373679770657	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA	8416	0.10232818506810976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.2158767237180343E-5	0.0	0.0	0.0
6	1.2158767237180343E-5	1.2158767237180343E-5	0.0	0.0	0.0
7	1.2158767237180343E-5	1.2158767237180343E-5	0.0	0.0	0.0
8	1.2158767237180343E-5	1.2158767237180343E-5	0.0	0.0	0.0
9	1.2158767237180343E-5	1.2158767237180343E-5	0.0	0.0	0.0
10-11	1.2158767237180343E-5	1.2158767237180343E-5	0.0	0.0	0.0
12-13	2.4317534474360685E-5	1.2158767237180343E-5	0.0	6.079383618590171E-6	0.0
14-15	2.4317534474360685E-5	1.2158767237180343E-5	0.0	1.2158767237180343E-5	0.0
16-17	2.4317534474360685E-5	1.2158767237180343E-5	0.0	1.2158767237180343E-5	0.0
18-19	3.0396918092950857E-5	1.2158767237180343E-5	0.0	1.2158767237180343E-5	0.0
20-21	3.6476301711541026E-5	2.4317534474360685E-5	0.0	1.2158767237180343E-5	0.0
22-23	3.6476301711541026E-5	2.4317534474360685E-5	0.0	1.2158767237180343E-5	0.0
24-25	3.6476301711541026E-5	2.4317534474360685E-5	0.0	1.2158767237180343E-5	0.0
26-27	3.6476301711541026E-5	2.4317534474360685E-5	0.0	1.2158767237180343E-5	0.0
28-29	3.6476301711541026E-5	2.4317534474360685E-5	0.0	1.2158767237180343E-5	0.0
30-31	3.6476301711541026E-5	2.4317534474360685E-5	0.0	2.4317534474360685E-5	0.0
32-33	3.6476301711541026E-5	2.4317534474360685E-5	0.0	3.0396918092950857E-5	0.0
34-35	4.25556853301312E-5	2.4317534474360685E-5	0.0	3.6476301711541026E-5	0.0
36-37	4.863506894872137E-5	2.4317534474360685E-5	0.0	3.6476301711541026E-5	0.0
38-39	5.471445256731154E-5	2.4317534474360685E-5	0.0	3.6476301711541026E-5	0.0
40-41	6.0793836185901715E-5	2.4317534474360685E-5	0.0	3.6476301711541026E-5	0.0
42-43	6.0793836185901715E-5	2.4317534474360685E-5	0.0	3.6476301711541026E-5	0.0
44-45	6.687321980449189E-5	2.4317534474360685E-5	0.0	3.6476301711541026E-5	6.079383618590171E-6
46-47	9.727013789744275E-5	2.4317534474360685E-5	0.0	3.6476301711541026E-5	1.2158767237180343E-5
48-49	1.702227413205248E-4	2.4317534474360685E-5	0.0	3.6476301711541026E-5	1.2158767237180343E-5
50-51	2.1885781026924618E-4	2.4317534474360685E-5	0.0	3.6476301711541026E-5	1.8238150855770513E-5
52-53	2.370959611250167E-4	2.4317534474360685E-5	0.0	3.6476301711541026E-5	2.4317534474360685E-5
54-55	2.7357226283655767E-4	2.4317534474360685E-5	0.0	5.471445256731154E-5	2.4317534474360685E-5
56-57	3.0396918092950853E-4	2.4317534474360685E-5	0.0	6.0793836185901715E-5	3.0396918092950857E-5
58-59	3.830011679711808E-4	2.4317534474360685E-5	0.0	6.0793836185901715E-5	3.6476301711541026E-5
60-61	4.25556853301312E-4	2.4317534474360685E-5	0.0	6.0793836185901715E-5	3.6476301711541026E-5
62-63	4.741919222500334E-4	2.4317534474360685E-5	0.0	7.295260342308205E-5	3.6476301711541026E-5
64-65	5.471445256731154E-4	2.4317534474360685E-5	0.0	7.295260342308205E-5	3.6476301711541026E-5
66-67	6.079383618590172E-4	2.4317534474360685E-5	0.0	7.295260342308205E-5	3.6476301711541026E-5
68-69	7.295260342308205E-4	2.4317534474360685E-5	0.0	7.295260342308205E-5	3.6476301711541026E-5
70-71	8.024786376539027E-4	2.4317534474360685E-5	0.0	7.295260342308205E-5	6.0793836185901715E-5
72-73	9.909395298301979E-4	2.4317534474360685E-5	0.0	7.295260342308205E-5	6.0793836185901715E-5
74-75	0.0011246859694391815	2.4317534474360685E-5	0.0	7.295260342308205E-5	7.295260342308205E-5
76-77	0.0012462736418109852	2.4317534474360685E-5	0.0	7.295260342308205E-5	7.295260342308205E-5
78-79	0.001489448986554592	2.4317534474360685E-5	0.0	7.295260342308205E-5	7.903198704167223E-5
80-81	0.0017083067968238382	2.4317534474360685E-5	0.0	7.295260342308205E-5	8.51113706602624E-5
82-83	0.0021521018009809205	2.4317534474360685E-5	0.0	7.295260342308205E-5	8.51113706602624E-5
84-85	0.0028451515335002004	2.4317534474360685E-5	0.0	7.295260342308205E-5	9.727013789744274E-5
86-87	0.003690185856484234	2.4317534474360685E-5	0.0	8.51113706602624E-5	9.727013789744274E-5
88-89	0.005027650252574073	2.4317534474360685E-5	0.0	8.51113706602624E-5	9.727013789744274E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGTA	211955	0.0	91.37182	5
AAGCAGT	213510	0.0	91.081245	1
GGTATCA	213090	0.0	91.07612	8
TGGTATC	213105	0.0	91.00957	7
GTATCAA	213225	0.0	91.007324	9
GTGGTAT	214330	0.0	90.53814	6
GCAGTGG	215625	0.0	90.05237	3
AGCAGTG	216585	0.0	89.81661	2
CAGTGGT	215960	0.0	89.67898	4
TCAACGC	212270	0.0	46.30769	12-13
AACGCAG	213095	0.0	46.167084	14-15
CAACGCA	212210	0.0	46.130653	12-13
ATCAACG	212135	0.0	46.123463	10-11
CGCAGAG	213730	0.0	46.000458	16-17
ACGCAGA	213390	0.0	45.88639	14-15
TATCAAC	213935	0.0	45.875175	10-11
CAGAGTA	212795	0.0	45.593292	18-19
AGAGTAC	209145	0.0	45.46997	18-19
GAGTACT	123045	0.0	45.22624	20-21
GCAGAGT	215675	0.0	45.21703	16-17
>>END_MODULE