FastQCFastQC Report
Sat 18 Jun 2016
SRR578587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8224518
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1032991.2559884968334922No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248760.30246149379209825No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92130.1120187225561425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3750.029.0827623
CGCGTAG2600.021.87969854-55
CTCGCGT2550.021.37918552-53
CAACGCA60700.020.89144912-13
ATCAACG61250.020.58775710-11
ACTCGCG2650.020.57242250-51
TCGCGTA3250.018.96240652-53
CGCTAGT3750.018.14664390-91
CGTACGC3200.017.77920220-21
ACGTACG3200.017.77920220-21
GGTATCA81500.017.6277838
AGTGGTA83500.017.3762285
TCAACGC60650.017.11775410-11
AAGCAGT87600.016.9982511
GTATCAA84200.016.7795339
ACGCAGA78650.016.18374414-15
TGGTATC89950.016.1299487
AACGCAG64600.016.10777312-13
GTGGTAT100200.015.1897276
CGTAGCG4050.014.63148656-57
Produced by FastQC (version 0.11.5)