##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578587_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8224518 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.984432887130893 31.0 31.0 34.0 30.0 34.0 2 32.25259960522914 34.0 31.0 34.0 30.0 34.0 3 32.32157203619714 34.0 31.0 34.0 30.0 34.0 4 35.89672403902575 37.0 35.0 37.0 35.0 37.0 5 35.777614201829216 37.0 35.0 37.0 33.0 37.0 6 35.75127405642495 37.0 35.0 37.0 33.0 37.0 7 35.746183302170415 37.0 35.0 37.0 33.0 37.0 8 35.66641534494787 37.0 35.0 37.0 33.0 37.0 9 37.30126203140415 39.0 37.0 39.0 33.0 39.0 10-11 37.29360067058033 39.0 37.0 39.0 33.0 39.0 12-13 37.28782768789611 39.0 37.0 39.0 33.0 39.0 14-15 38.5990907309097 40.0 38.0 41.0 34.0 41.0 16-17 38.54880194802905 40.0 38.0 41.0 34.0 41.0 18-19 38.48623992311768 40.0 38.0 41.0 33.5 41.0 20-21 38.42262227403478 40.0 38.0 41.0 34.0 41.0 22-23 38.29997387080921 40.0 38.0 41.0 33.0 41.0 24-25 38.21432319802814 40.0 37.5 41.0 33.0 41.0 26-27 38.04715802190475 40.0 37.0 41.0 33.0 41.0 28-29 37.95150189956421 40.0 37.0 41.0 33.0 41.0 30-31 37.85458862148518 40.0 37.0 41.0 33.0 41.0 32-33 37.720440213031324 40.0 37.0 41.0 32.0 41.0 34-35 37.59101451294775 40.0 37.0 41.0 32.0 41.0 36-37 37.46726057381113 40.0 36.5 41.0 31.5 41.0 38-39 37.34148347660009 40.0 36.0 41.0 31.0 41.0 40-41 37.1957759713092 39.5 36.0 41.0 31.0 41.0 42-43 37.03903189220328 39.0 36.0 41.0 31.0 41.0 44-45 36.887496446600274 39.0 35.0 41.0 30.0 41.0 46-47 36.96725096838502 39.0 36.0 41.0 31.0 41.0 48-49 36.95104065672906 39.0 36.0 41.0 31.0 41.0 50-51 36.93209091402073 39.0 36.0 41.0 31.0 41.0 52-53 36.94959923487309 39.0 36.0 41.0 31.0 41.0 54-55 36.82800268660121 39.0 35.0 41.0 31.0 41.0 56-57 36.686937945786 39.0 35.0 41.0 30.5 41.0 58-59 36.47834371570468 39.0 35.0 41.0 30.0 41.0 60-61 36.258179324308124 39.0 35.0 41.0 30.0 41.0 62-63 35.99625139126694 38.0 35.0 40.0 29.0 41.0 64-65 35.69439613360929 38.0 34.5 40.0 29.0 41.0 66-67 35.38342046305935 37.0 34.0 40.0 29.0 41.0 68-69 35.05660824135834 37.0 34.0 39.5 28.5 41.0 70-71 34.69563413928938 36.0 34.0 39.0 28.0 41.0 72-73 34.274024447390104 36.0 34.0 39.0 28.0 40.5 74-75 33.88872150319325 35.0 33.0 38.0 27.0 40.0 76-77 32.44353577923958 34.5 31.5 36.5 26.0 39.0 78-79 33.04350966220756 35.0 32.5 37.0 26.0 39.0 80-81 32.98419481846839 35.0 33.0 36.0 26.0 39.0 82-83 32.70016613739553 35.0 33.0 36.0 26.0 37.5 84-85 32.40964023666797 35.0 33.0 36.0 26.0 37.0 86-87 32.11970902124598 35.0 32.5 35.0 26.0 37.0 88-89 31.863739492089383 35.0 32.0 35.0 25.0 36.0 90-91 31.61632055519849 35.0 32.0 35.0 25.0 36.0 92-93 31.374750897256227 35.0 32.0 35.0 24.5 36.0 94-95 31.118899490523333 35.0 32.0 35.0 24.0 36.0 96-97 30.515041793330624 34.0 31.5 35.0 18.5 35.0 98-99 30.488320835336488 34.0 31.5 35.0 19.0 35.0 100-101 29.91167154111645 34.0 30.5 35.0 9.5 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.429129689136694 1101 2 0.40659333679636234 1101 3 0.4093937994192558 1101 4 0.3093981069689562 1101 5 0.2876062206035641 1101 6 0.3097063037691541 1101 7 0.34485828384665496 1101 8 0.37772292244884653 1101 9 0.4938204623251252 1101 10-11 0.4350670545594042 1101 12-13 0.421554814543029 1101 14-15 0.5884286138939245 1101 16-17 0.5933006834953929 1101 18-19 0.5848429994414559 1101 20-21 0.6306229971663697 1101 22-23 0.6753580174744016 1101 24-25 0.6988775049934404 1101 26-27 0.7073206338929552 1101 28-29 0.6979859299834956 1101 30-31 0.7010627475561861 1101 32-33 0.6952832556398363 1101 34-35 0.691375297269083 1101 36-37 0.6765672576551154 1101 38-39 0.7757721119847645 1101 40-41 0.8299464478923824 1101 42-43 0.8444335073702618 1101 44-45 0.7806604599536797 1101 46-47 0.7970323573817453 1101 48-49 0.6998916271510112 1101 50-51 0.7167970721976715 1101 52-53 0.7183832126346843 1101 54-55 0.7883290344014071 1101 56-57 0.8280749218885006 1101 58-59 0.8177646249954407 1101 60-61 0.7426162894814823 1101 62-63 0.7832441016916007 1101 64-65 0.7608484145083168 1101 66-67 0.7159797312753042 1101 68-69 0.7655831547047782 1101 70-71 0.6978423361595105 1101 72-73 0.6986576561790088 1101 74-75 0.749916403702791 1101 76-77 0.7167817798228171 1101 78-79 0.6358584520438626 1101 80-81 0.5780050620091188 1101 82-83 0.5906723028008614 1101 84-85 0.5154684473114273 1101 86-87 0.507288932633017 1101 88-89 0.530086707787472 1101 90-91 0.5631275610539639 1101 92-93 0.5913219381218262 1101 94-95 0.6260999052720422 1101 96-97 0.9477805714145298 1101 98-99 0.6955821982153338 1101 100-101 0.6597062703611947 1102 1 0.39716150348051826 1102 2 0.362640127196336 1102 3 0.335859335305706 1102 4 0.24174514187846086 1102 5 0.28475984126308873 1102 6 0.2935173899120258 1102 7 0.2583436261050167 1102 8 0.2936989871528368 1102 9 0.4475340623583577 1102 10-11 0.39393120330331044 1102 12-13 0.3982909849689875 1102 14-15 0.48834345712555915 1102 16-17 0.48914091771622026 1102 18-19 0.5005552480127022 1102 20-21 0.5598940813785802 1102 22-23 0.559326415747563 1102 24-25 0.5545273125994967 1102 26-27 0.605231568149101 1102 28-29 0.6054172296475784 1102 30-31 0.6569400736654245 1102 32-33 0.6921777646229046 1102 34-35 0.7106813716385929 1102 36-37 0.741408198155348 1102 38-39 0.7460903470682752 1102 40-41 0.7470993109511568 1102 42-43 0.7531629742515022 1102 44-45 0.7577422673935672 1102 46-47 0.8197640762923157 1102 48-49 0.83129480149816 1102 50-51 0.8113863670546593 1102 52-53 0.7428512180552005 1102 54-55 0.7347934399964018 1102 56-57 0.6870000391638769 1102 58-59 0.7381315848047194 1102 60-61 0.7462891929130464 1102 62-63 0.7282116348335848 1102 64-65 0.7081999828029666 1102 66-67 0.7354697682144504 1102 68-69 0.7407416704427092 1102 70-71 0.7132316635200127 1102 72-73 0.7299898176750474 1102 74-75 0.6131610061029136 1102 76-77 0.5513940348703343 1102 78-79 0.6086420299478306 1102 80-81 0.59181245179348 1102 82-83 0.5963731690317857 1102 84-85 0.6024397977836529 1102 86-87 0.6231199229027311 1102 88-89 0.5960935591865244 1102 90-91 0.5812980346644103 1102 92-93 0.6074839266038516 1102 94-95 0.58944002035404 1102 96-97 1.0182990513741608 1102 98-99 0.7870497380779646 1102 100-101 0.7658538766483041 1103 1 0.33807826860039825 1103 2 0.28172515567953127 1103 3 0.2741382561805139 1103 4 0.23660623511852918 1103 5 0.20221081936163898 1103 6 0.2853948244357696 1103 7 0.28550127211795484 1103 8 0.10828714079443813 1103 9 0.2066947341017027 1103 10-11 0.3645173995780766 1103 12-13 0.36715563422210806 1103 14-15 0.4321738914504394 1103 16-17 0.44230588630867373 1103 18-19 0.3756480669718698 1103 20-21 0.4021247192384223 1103 22-23 0.442694919297729 1103 24-25 0.27037633021191 1103 26-27 0.2707293319939623 1103 28-29 0.4545975335463268 1103 30-31 0.3008228928975285 1103 32-33 0.4894686441512661 1103 34-35 0.47167857595727725 1103 36-37 0.5282639144853292 1103 38-39 0.5799422461430055 1103 40-41 0.440520842766162 1103 42-43 0.5592341646970524 1103 44-45 0.6584533530303176 1103 46-47 0.5468466054286196 1103 48-49 0.5566413763011511 1103 50-51 0.49659333325303834 1103 52-53 0.5739449065219375 1103 54-55 0.6519590597447547 1103 56-57 0.5804209276212617 1103 58-59 0.3214899505983695 1103 60-61 0.3200702446662902 1103 62-63 0.2270953703629104 1103 64-65 0.44661036308581004 1103 66-67 0.417701283380147 1103 68-69 0.3824059001486759 1103 70-71 0.379402983806834 1103 72-73 0.5355776515874027 1103 74-75 0.48105716818224664 1103 76-77 0.3741382280803762 1103 78-79 0.49467966831845445 1103 80-81 0.5156685229449423 1103 82-83 0.5163267498043567 1103 84-85 0.5052571388060798 1103 86-87 0.3831099216160112 1103 88-89 0.3526566842669432 1103 90-91 0.45734709750680125 1103 92-93 0.45404443926240035 1103 94-95 0.4771551649925314 1103 96-97 0.552545640603963 1103 98-99 0.35027177054217873 1103 100-101 0.5644064584619493 1104 1 0.327091102577171 1104 2 0.29353745886679405 1104 3 0.29053727727310985 1104 4 0.21523430019965417 1104 5 0.2317032118603919 1104 6 0.24270163795959832 1104 7 0.2220921774007394 1104 8 0.33310574863967446 1104 9 0.31630900221475144 1104 10-11 0.337763829386283 1104 12-13 0.3271893478342207 1104 14-15 0.4091064621085323 1104 16-17 0.4307204322179601 1104 18-19 0.46456603032996924 1104 20-21 0.3995980708133615 1104 22-23 0.5204970818745025 1104 24-25 0.465110099712426 1104 26-27 0.5684666609585136 1104 28-29 0.5543352948910041 1104 30-31 0.5806178678885487 1104 32-33 0.6168679256453302 1104 34-35 0.5655164087423756 1104 36-37 0.5981124246593907 1104 38-39 0.614808380878344 1104 40-41 0.6253716149666673 1104 42-43 0.6731174750435471 1104 44-45 0.6220015427757133 1104 46-47 0.48106746540288015 1104 48-49 0.5722235062142502 1104 50-51 0.4927753272402171 1104 52-53 0.5663964720905525 1104 54-55 0.6720815654644738 1104 56-57 0.671650605096076 1104 58-59 0.6541388220130671 1104 60-61 0.5879753904690048 1104 62-63 0.45882887990780574 1104 64-65 0.6046054831868446 1104 66-67 0.6561239210913428 1104 68-69 0.6849902908993428 1104 70-71 0.6428096877644052 1104 72-73 0.6779053249071652 1104 74-75 0.6478953943002566 1104 76-77 0.5772606530678317 1104 78-79 0.5444418202414028 1104 80-81 0.701472630526375 1104 82-83 0.760547165435419 1104 84-85 0.7329439393347315 1104 86-87 0.6437843142321071 1104 88-89 0.5940864889442956 1104 90-91 0.6748106568125571 1104 92-93 0.6775741768758756 1104 94-95 0.7006255664406424 1104 96-97 1.0720143413618821 1104 98-99 0.8138062027382453 1104 100-101 0.8732863806914821 1105 1 0.14815309488980688 1105 2 0.15541037107701783 1105 3 0.11744405384257561 1105 4 0.054701356065251616 1105 5 0.10934808794844741 1105 6 0.08559826860921049 1105 7 0.11811194109045431 1105 8 0.189885866966236 1105 9 0.1534187921008794 1105 10-11 0.14392038930516549 1105 12-13 0.1653736424274399 1105 14-15 0.1700967064926573 1105 16-17 0.1615778641651744 1105 18-19 0.08271047566694989 1105 20-21 0.17928932974696465 1105 22-23 0.20171542811620924 1105 24-25 0.19141294640967743 1105 26-27 0.1596469812514414 1105 28-29 0.22289943387549016 1105 30-31 0.15984213626693844 1105 32-33 0.19654943760499322 1105 34-35 0.15084089443012516 1105 36-37 0.1807239081530767 1105 38-39 0.16992277252620624 1105 40-41 0.20741039290581398 1105 42-43 0.1400313861712661 1105 44-45 0.24678356402893797 1105 46-47 0.26625591846886465 1105 48-49 0.2359617597293635 1105 50-51 0.1968390645162046 1105 52-53 0.23877914395428235 1105 54-55 0.331971960175828 1105 56-57 0.311590162523963 1105 58-59 0.31225173985613 1105 60-61 0.2852516637701896 1105 62-63 0.3298631684304141 1105 64-65 0.30918277831940344 1105 66-67 0.3684038757169006 1105 68-69 0.33266261293595534 1105 70-71 0.32047102603808497 1105 72-73 0.3079174193717762 1105 74-75 0.3388784942091476 1105 76-77 0.34652250821447694 1105 78-79 0.29869397304052114 1105 80-81 0.2469642938177259 1105 82-83 0.24330047590019177 1105 84-85 0.34246854626463374 1105 86-87 0.2510901387148863 1105 88-89 0.20657620184915615 1105 90-91 0.2201837413657124 1105 92-93 0.21618331486228826 1105 94-95 0.2945193139410307 1105 96-97 0.7020498115634162 1105 98-99 0.5030881865194523 1105 100-101 0.4671528564987959 1106 1 -0.06307802264750251 1106 2 -0.11403656083896863 1106 3 -0.07536239801077471 1106 4 -0.039821686260715694 1106 5 -0.06555863269317541 1106 6 -0.02956034199014823 1106 7 -0.05182953286678327 1106 8 -0.05067240579684551 1106 9 -0.0011790602898287261 1106 10-11 -0.09321349806504031 1106 12-13 -0.09765295086958758 1106 14-15 -0.15406817752926116 1106 16-17 -0.14309943890060595 1106 18-19 -0.14347325995453986 1106 20-21 -0.16911742623612014 1106 22-23 -0.15264145214715796 1106 24-25 -0.047316927580325796 1106 26-27 -0.08021463457445321 1106 28-29 -0.11601665347345858 1106 30-31 -0.20184960710002287 1106 32-33 -0.2622397574575146 1106 34-35 -0.31256809054796264 1106 36-37 -0.26923683366121764 1106 38-39 -0.2991665288754035 1106 40-41 -0.306791272148601 1106 42-43 -0.2303761003496234 1106 44-45 -0.16921479284051344 1106 46-47 -0.2546231812548072 1106 48-49 -0.23738176114156317 1106 50-51 -0.26256570613524133 1106 52-53 -0.1853177031624611 1106 54-55 -0.21064658545272863 1106 56-57 -0.21836445086152168 1106 58-59 -0.15451508434929906 1106 60-61 -0.25324004430148506 1106 62-63 -0.1518335641085713 1106 64-65 -0.1424001841170508 1106 66-67 -0.20968481268163686 1106 68-69 -0.17060498565783888 1106 70-71 -0.14993314636691224 1106 72-73 -0.13433412537891343 1106 74-75 -0.13140589604022068 1106 76-77 -0.2281465393386739 1106 78-79 -0.13267844211516433 1106 80-81 -0.24025131802955713 1106 82-83 -0.20266498000601274 1106 84-85 -0.19215645926085756 1106 86-87 -0.259886030304159 1106 88-89 -0.2617302774867518 1106 90-91 -0.18176271230271723 1106 92-93 -0.18186967456212244 1106 94-95 -0.1557731881181823 1106 96-97 0.10119803289475371 1106 98-99 -0.0763847082953788 1106 100-101 -0.0696495146856293 1107 1 -0.15981810950391306 1107 2 -0.23999784221296494 1107 3 -0.187969682859638 1107 4 -0.13730902049880456 1107 5 -0.14575408622472708 1107 6 -0.12989096210476703 1107 7 -0.15587661225664817 1107 8 -0.12480371804991819 1107 9 -0.16249378312295448 1107 10-11 -0.1795131254008595 1107 12-13 -0.16498164575761365 1107 14-15 -0.21643708169060716 1107 16-17 -0.23597045064572342 1107 18-19 -0.2514914965887627 1107 20-21 -0.24376243857373225 1107 22-23 -0.18090022721043653 1107 24-25 -0.13302577081622502 1107 26-27 -0.12056349968781177 1107 28-29 -0.1612190551982664 1107 30-31 -0.20583934075209953 1107 32-33 -0.2785528435696065 1107 34-35 -0.28753026487004973 1107 36-37 -0.2695056232673565 1107 38-39 -0.2847758806895939 1107 40-41 -0.23795078514491763 1107 42-43 -0.3061873498438601 1107 44-45 -0.17595079212360787 1107 46-47 -0.24696983049788201 1107 48-49 -0.31710582621196437 1107 50-51 -0.2602582049753508 1107 52-53 -0.29100475041281726 1107 54-55 -0.28943533647070296 1107 56-57 -0.21876375845521778 1107 58-59 -0.21497257601276942 1107 60-61 -0.27634632153662864 1107 62-63 -0.4045829840576687 1107 64-65 -0.375441197042484 1107 66-67 -0.27809967526926016 1107 68-69 -0.2894770854488513 1107 70-71 -0.2575263900360838 1107 72-73 -0.21363450297252484 1107 74-75 -0.1878520716433556 1107 76-77 -0.3203105344741388 1107 78-79 -0.1128497377994293 1107 80-81 -0.14327366167990618 1107 82-83 -0.1874813820700254 1107 84-85 -0.17643172102255988 1107 86-87 -0.2151759621237801 1107 88-89 -0.21899589764348448 1107 90-91 -0.18668027217299255 1107 92-93 -0.13394658244084567 1107 94-95 -0.172179113682688 1107 96-97 0.13037735780466164 1107 98-99 -0.20363937315883263 1107 100-101 -0.29197691559836514 1108 1 -0.36480281677051707 1108 2 -0.36201646375247876 1108 3 -0.35885607375789164 1108 4 -0.2812734778247261 1108 5 -0.28382092220369515 1108 6 -0.29529971129711896 1108 7 -0.28977875528914154 1108 8 -0.2599244135001939 1108 9 -0.3509325296350525 1108 10-11 -0.4319200045520688 1108 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-0.15720905429950705 2305 16-17 -0.13773781950791175 2305 18-19 -0.18046091151736476 2305 20-21 -0.1804543294495673 2305 22-23 -0.169249411466474 2305 24-25 -0.1051724600303956 2305 26-27 -0.2060329948656161 2305 28-29 -0.18079243286809543 2305 30-31 -0.21599322159307377 2305 32-33 -0.1850930737786598 2305 34-35 -0.17802979177030664 2305 36-37 -0.152358309494943 2305 38-39 -0.16293526260760416 2305 40-41 -0.21025496841301106 2305 42-43 -0.258955326413691 2305 44-45 -0.23451100403162428 2305 46-47 -0.09407884901036567 2305 48-49 -0.146672374793539 2305 50-51 -0.16033288046968863 2305 52-53 -0.2438493244241684 2305 54-55 -0.1301869344107658 2305 56-57 -0.17136319022237956 2305 58-59 -0.15147861799771078 2305 60-61 -0.13389997879922788 2305 62-63 -0.12059250390776555 2305 64-65 -0.10672844416695426 2305 66-67 -0.12664666351239617 2305 68-69 -0.09066763088409857 2305 70-71 -0.06460743654962897 2305 72-73 -0.08371661248217066 2305 74-75 -0.11792833959116678 2305 76-77 -0.12846659000938132 2305 78-79 -0.0835052949470878 2305 80-81 -0.11015325196969883 2305 82-83 -0.07584304555714283 2305 84-85 -0.0577762351319393 2305 86-87 -0.023104252597889285 2305 88-89 -0.022867994976667916 2305 90-91 -0.05416412211829069 2305 92-93 0.018842562995804002 2305 94-95 -0.10014836424632634 2305 96-97 0.14197280643325527 2305 98-99 -0.13376906747901174 2305 100-101 -0.11338526532453841 2306 1 -0.21617847166711712 2306 2 -0.2847337565909669 2306 3 -0.2580431797370508 2306 4 -0.1844606536489266 2306 5 -0.21037217160134247 2306 6 -0.14488759471516488 2306 7 -0.20787474531667272 2306 8 -0.1952180840792579 2306 9 -0.34540129291745814 2306 10-11 -0.27709590972593645 2306 12-13 -0.2937488822491616 2306 14-15 -0.39338621831021214 2306 16-17 -0.37233748481855145 2306 18-19 -0.3884719983972431 2306 20-21 -0.4680109910348875 2306 22-23 -0.3755028705704362 2306 24-25 -0.37941960596614166 2306 26-27 -0.44660867035736374 2306 28-29 -0.500582758103171 2306 30-31 -0.547271231301373 2306 32-33 -0.6477742795723813 2306 34-35 -0.5600322899473653 2306 36-37 -0.5772101819769162 2306 38-39 -0.7068540454302905 2306 40-41 -0.622386173009815 2306 42-43 -0.7182384417615353 2306 44-45 -0.6914344606690506 2306 46-47 -0.6204773904053837 2306 48-49 -0.6269835191027582 2306 50-51 -0.6624105866935395 2306 52-53 -0.6042253492700738 2306 54-55 -0.6347899698947259 2306 56-57 -0.6178958150101934 2306 58-59 -0.6372186552124788 2306 60-61 -0.5939511713523657 2306 62-63 -0.49699907944727073 2306 64-65 -0.5660873967971298 2306 66-67 -0.5590357092408098 2306 68-69 -0.5781061789296515 2306 70-71 -0.6623619554385556 2306 72-73 -0.671736829474483 2306 74-75 -0.6279550228543016 2306 76-77 -0.5839709450749275 2306 78-79 -0.5557380016007869 2306 80-81 -0.5416966378288137 2306 82-83 -0.5331800872946175 2306 84-85 -0.5042269437395319 2306 86-87 -0.4925746502722319 2306 88-89 -0.5248605143007943 2306 90-91 -0.5770497595863411 2306 92-93 -0.5973194185295903 2306 94-95 -0.6153525590517113 2306 96-97 -0.3452042606863124 2306 98-99 -0.6253945720784309 2306 100-101 -0.6110199588225242 2307 1 -0.49942404865266 2307 2 -0.5144043128272244 2307 3 -0.5269053730939 2307 4 -0.4192242537266324 2307 5 -0.44831692828062586 2307 6 -0.41168923801853907 2307 7 -0.4460723678738674 2307 8 -0.4254645769997083 2307 9 -0.49941124421315664 2307 10-11 -0.617585900785997 2307 12-13 -0.6072076239221502 2307 14-15 -0.9576412705669668 2307 16-17 -0.8181213467287236 2307 18-19 -0.7963594597172374 2307 20-21 -0.6700387641764891 2307 22-23 -0.6963139304273582 2307 24-25 -0.6050993011733397 2307 26-27 -0.7472528882469831 2307 28-29 -0.7096290655144557 2307 30-31 -0.7701608257451724 2307 32-33 -0.7905454865440262 2307 34-35 -0.8318161073134789 2307 36-37 -0.8899030130027867 2307 38-39 -0.8657487145236971 2307 40-41 -0.7570414665867418 2307 42-43 -0.8919090311894564 2307 44-45 -0.825384410242556 2307 46-47 -0.852600134202369 2307 48-49 -0.7453557343817394 2307 50-51 -0.8195203494351659 2307 52-53 -0.8942317375701165 2307 54-55 -0.9545880024774789 2307 56-57 -0.9431377699564507 2307 58-59 -0.9815559881848017 2307 60-61 -1.0134691311184625 2307 62-63 -0.9900611639493846 2307 64-65 -0.9563846935375864 2307 66-67 -0.9005535910463962 2307 68-69 -0.8554451200174569 2307 70-71 -0.9440262889284625 2307 72-73 -1.2409235138093564 2307 74-75 -1.053833282461774 2307 76-77 -0.9541161298446674 2307 78-79 -0.9468310483309139 2307 80-81 -0.834719917416642 2307 82-83 -0.8784488761808049 2307 84-85 -0.924711078319298 2307 86-87 -0.8837533161612114 2307 88-89 -0.8718839135719492 2307 90-91 -0.9633630685468226 2307 92-93 -0.8772050445178614 2307 94-95 -0.8763646912860992 2307 96-97 -0.7204269823371625 2307 98-99 -1.0491887816397814 2307 100-101 -1.0858819354949993 2308 1 -1.408533954487769 2308 2 -0.8642190795988185 2308 3 -0.8740719651667739 2308 4 -0.5428001156363962 2308 5 -0.5918110492904489 2308 6 -0.6127003558362105 2308 7 -0.6193927673058752 2308 8 -0.5962809221416094 2308 9 -0.871676321502143 2308 10-11 -0.8064396733389501 2308 12-13 -0.7485812157646805 2308 14-15 -1.0044840400241455 2308 16-17 -0.9109615216966418 2308 18-19 -0.9200830981179919 2308 20-21 -0.9784486269453012 2308 22-23 -1.0183470845815705 2308 24-25 -1.1911759679415468 2308 26-27 -1.1880200565972459 2308 28-29 -1.2385639508149353 2308 30-31 -1.1479398760523694 2308 32-33 -1.1827864239212573 2308 34-35 -1.2410198766464404 2308 36-37 -1.2972780279674083 2308 38-39 -1.2963874982161627 2308 40-41 -1.2587572154031506 2308 42-43 -1.186675715427235 2308 44-45 -1.2299242672167807 2308 46-47 -1.2149122244372208 2308 48-49 -1.2572569943165632 2308 50-51 -1.2911463168841664 2308 52-53 -1.2243447172311548 2308 54-55 -1.1994791615433087 2308 56-57 -1.0899957753564706 2308 58-59 -1.1048795512727594 2308 60-61 -1.0500469851754843 2308 62-63 -1.0696760235457887 2308 64-65 -1.0368007430027504 2308 66-67 -1.037929593278129 2308 68-69 -1.181737540767763 2308 70-71 -1.117400835842382 2308 72-73 -1.1351056963415687 2308 74-75 -1.2321781367003695 2308 76-77 -1.1526601898733944 2308 78-79 -1.5475605898075955 2308 80-81 -1.2149296936277416 2308 82-83 -1.1718741361624652 2308 84-85 -1.0794427658427992 2308 86-87 -1.044837906776877 2308 88-89 -1.090063838840294 2308 90-91 -1.1489157705636117 2308 92-93 -1.0606578343419564 2308 94-95 -1.1447467193770677 2308 96-97 -1.0124398692519279 2308 98-99 -1.3491244240448559 2308 100-101 -1.384044051882011 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37.0 3 77.0 4 179.0 5 506.0 6 999.0 7 1858.0 8 3567.0 9 5475.0 10 7456.0 11 9258.0 12 10755.0 13 12122.0 14 13523.0 15 15212.0 16 17306.0 17 19808.0 18 22906.0 19 26255.0 20 28201.0 21 29376.0 22 32403.0 23 37645.0 24 46209.0 25 57910.0 26 73866.0 27 94882.0 28 120409.0 29 151266.0 30 187546.0 31 231291.0 32 285994.0 33 359056.0 34 472678.0 35 698634.0 36 950408.0 37 1610456.0 38 1985042.0 39 601801.0 40 2146.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.275856869462732 31.330252281300712 28.29443997627316 22.09945087296339 2 22.670243137422773 29.16508370906788 28.396860806586012 19.76781234692334 3 21.142547180043646 28.359096053661297 28.663533246725326 21.834823519569728 4 21.54951615768395 28.85494117133527 28.34073218272724 21.25481048825354 5 22.157777403554167 28.667075610665048 28.35826412391854 20.816882861862243 6 21.932798979830796 28.497645697899866 28.177906595863732 21.391648726405606 7 22.100966889488234 28.74242599991878 28.118316477634313 21.038290632958674 8 21.35258401165965 28.6180261038595 28.431922594558944 21.59746728992191 9 21.205495489919425 28.440508876247634 28.8392958439941 21.514699789838843 10-11 21.228180180285335 28.90883696771045 28.356190599862508 21.506792252141707 12-13 21.27438957517024 28.91737242231095 28.16709745178988 21.64114055072893 14-15 21.413035997975808 28.93935547347577 28.021009863435157 21.62659866511326 16-17 21.5935815326807 28.757405601154986 27.959456104296933 21.689556761867383 18-19 21.58530141219218 28.76584378561759 27.912644850433786 21.736209951756443 20-21 21.6788692054659 28.678507360552924 27.974089180666876 21.6685342533143 22-23 21.65651155615606 28.66834356443619 27.973372281943764 21.701772597463982 24-25 21.63157666269421 28.639083954948706 27.889345714936415 21.839993667420668 26-27 21.8041417375609 28.511301329719842 28.0328716432952 21.651685289424062 28-29 21.816761784702763 28.598460116446944 27.929831267923543 21.65494683092675 30-31 21.781142676081444 28.604977215686073 27.876350930230803 21.737529178001676 32-33 21.825753193074657 28.63159883655188 27.80030999992948 21.74233797044398 34-35 21.831942005598382 28.624248861757007 27.80595166792753 21.73785746471708 36-37 21.860442156002332 28.63138605812523 27.77055141711648 21.737620368755955 38-39 21.86894721368474 28.625756548894415 27.752155202286627 21.753141035134217 40-41 21.855438823284235 28.677601532393755 27.744568131530627 21.72239151279139 42-43 21.89719202997671 28.672203039740445 27.70691242939708 21.723692500885765 44-45 21.890826915328045 28.682568388810143 27.696103285323225 21.73050141053859 46-47 21.863799304944028 28.718450656831845 27.67975142614749 21.737998612076638 48-49 21.913338872867687 28.69437455179744 27.640896402682806 21.751390172652062 50-51 21.907101425275012 28.69054454011773 27.641638087484278 21.76071594712298 52-53 21.917594441400702 28.754748910513662 27.55895847027145 21.768698177814187 54-55 21.921661549041538 28.773692269869187 27.50229861494619 21.802347566143084 56-57 21.92794763170316 28.770834959568454 27.521752642525676 21.779464766202715 58-59 21.926470341483842 28.856013203448516 27.4780053980063 21.739511057061335 60-61 21.91382563656368 28.832534846144654 27.453869293870483 21.799770223421184 62-63 21.904872607577257 28.842090007086142 27.48885404452157 21.764183340815038 64-65 21.93269809247793 28.918351431310285 27.430156172563606 21.718794303648174 66-67 21.904078696553377 28.92390147610752 27.427348949070172 21.74467087826893 68-69 21.89232136067752 28.960819581418473 27.42088841728929 21.72597064061472 70-71 21.912194107730496 29.006535955659334 27.384748383033386 21.69652155357678 72-73 21.890576148329252 28.983311354324403 27.377149579902227 21.74896291744412 74-75 21.95073019547669 29.005618571805492 27.375369474898807 21.66828175781901 76-77 21.922277450378203 29.06125938659353 27.35359281916427 21.662870343864 78-79 21.925424426579088 29.07200639517778 27.28716444340524 21.715404734837897 80-81 21.901164932622123 29.09781859344812 27.299012342376628 21.702004131553128 82-83 21.934413366854145 29.15501401418849 27.230702365483637 21.679870253473727 84-85 21.90628143343262 29.184951155972083 27.239860073193086 21.66890733740221 86-87 21.88216179440205 29.21897384292731 27.220419888970106 21.67844447370053 88-89 21.882680751101404 29.269137810301967 27.20327353288542 21.644907905711218 90-91 21.86001825899091 29.253430105782208 27.187728789143968 21.698822846082916 92-93 21.863054835246835 29.285805783554103 27.172126536433115 21.67901284476595 94-95 21.850547318887887 29.348841036567574 27.14592618172908 21.65468546281546 96-97 21.85809402669687 29.35894681592227 27.129109919438726 21.653849237942133 98-99 21.859763023665877 29.39557735192812 27.096838526851514 21.64782109755449 100-101 21.87194929837703 29.451063970355673 26.992440662893376 21.684546068373923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 121030.0 1 68200.5 2 11377.0 3 6675.5 4 5519.0 5 4666.0 6 4327.5 7 4290.0 8 3966.5 9 3733.0 10 3764.0 11 4268.5 12 7785.0 13 7734.0 14 4644.5 15 4865.0 16 5467.5 17 6481.0 18 7913.5 19 9567.5 20 11984.0 21 15876.0 22 20886.5 23 27183.5 24 35435.5 25 45977.5 26 58560.0 27 72146.5 28 84275.0 29 96575.0 30 110390.5 31 125037.0 32 139969.5 33 154707.5 34 171762.5 35 187241.5 36 203559.0 37 221390.0 38 234980.0 39 244895.0 40 256943.5 41 268496.5 42 275994.5 43 287891.5 44 300423.0 45 309894.0 46 314730.5 47 313014.5 48 311670.0 49 311736.0 50 307599.5 51 298130.0 52 287130.0 53 271096.0 54 249987.5 55 232316.0 56 213607.0 57 190459.5 58 170866.5 59 149148.5 60 121021.5 61 92545.5 62 66137.5 63 45465.0 64 31210.0 65 20338.0 66 12447.0 67 7694.5 68 4229.0 69 1941.5 70 855.5 71 381.5 72 193.0 73 112.0 74 68.0 75 39.5 76 30.0 77 23.0 78 13.5 79 10.5 80 10.0 81 6.5 82 4.0 83 4.0 84 2.5 85 1.5 86 1.0 87 1.5 88 2.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011186065858205916 2 0.01840837359709104 3 0.0059577959462183685 4 4.98509456724394E-4 5 0.0010091776806859683 6 0.0 7 0.0 8 0.007295260342308206 9 0.010079618039622505 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.687321980449189E-5 24-25 0.0046324903173657105 26-27 0.0064441466357055815 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 6.079383618590172E-6 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 8.51113706602624E-5 62-63 1.2158767237180343E-4 64-65 3.161279481666889E-4 66-67 1.884608921762953E-4 68-69 3.2828671540386924E-4 70-71 1.5806397408334446E-4 72-73 4.863506894872138E-5 74-75 9.727013789744275E-5 76-77 0.12413493410799271 78-79 0.05562028072648148 80-81 9.727013789744275E-5 82-83 0.0021217048828879696 84-85 0.009605426117372471 86-87 0.11798259788597946 88-89 0.13382547159602545 90-91 0.14743113213443026 92-93 0.14578361917379232 94-95 0.11294278886616821 96-97 1.2123324430683962 98-99 0.07573696112039635 100-101 0.06820460481696314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8224518.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.8403836017759 #Duplication Level Percentage of deduplicated Percentage of total 1 92.0526510454679 67.97203064757767 2 4.802743629403415 7.092728638722671 3 1.112157517105391 2.4636641306598213 4 0.5208849406732595 1.5384937532680705 5 0.30066157081331507 1.1100482861583847 6 0.19831441459395058 0.878616746842736 7 0.12923545707546868 0.6679957007782416 8 0.09370343065693608 0.5535277811608454 9 0.0702426405995912 0.4668069174367943 >10 0.696009022168524 10.976320512930108 >50 0.022168589223006966 0.9869477984257318 >100 9.460973762532663E-4 0.13071583631122707 >500 0.0 0.0 >1k 9.38816143188794E-5 0.21389470995816423 >5k 9.38816143188794E-5 0.5054999919024254 >10k+ 9.38816143188794E-5 4.442708547867072 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103299 1.2559884968334922 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24876 0.30246149379209825 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 9213 0.1120187225561425 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.2158767237180343E-5 3 0.0 0.0 0.0 0.0 1.2158767237180343E-5 4 0.0 0.0 0.0 0.0 1.2158767237180343E-5 5 0.0 0.0 0.0 1.2158767237180343E-5 1.2158767237180343E-5 6 0.0 0.0 0.0 1.2158767237180343E-5 1.2158767237180343E-5 7 0.0 0.0 0.0 2.4317534474360685E-5 2.4317534474360685E-5 8 0.0 0.0 0.0 2.4317534474360685E-5 2.4317534474360685E-5 9 0.0 0.0 0.0 2.4317534474360685E-5 2.4317534474360685E-5 10-11 0.0 0.0 0.0 3.0396918092950857E-5 2.4317534474360685E-5 12-13 0.0 0.0 0.0 3.6476301711541026E-5 2.4317534474360685E-5 14-15 6.079383618590171E-6 0.0 0.0 3.6476301711541026E-5 2.4317534474360685E-5 16-17 1.2158767237180343E-5 0.0 0.0 4.25556853301312E-5 2.4317534474360685E-5 18-19 1.8238150855770513E-5 0.0 0.0 4.863506894872137E-5 2.4317534474360685E-5 20-21 2.4317534474360685E-5 0.0 0.0 4.863506894872137E-5 2.4317534474360685E-5 22-23 3.6476301711541026E-5 0.0 0.0 4.863506894872137E-5 2.4317534474360685E-5 24-25 3.6476301711541026E-5 0.0 0.0 4.863506894872137E-5 2.4317534474360685E-5 26-27 3.6476301711541026E-5 0.0 0.0 4.863506894872137E-5 2.4317534474360685E-5 28-29 4.863506894872137E-5 0.0 0.0 6.0793836185901715E-5 3.6476301711541026E-5 30-31 5.471445256731154E-5 0.0 0.0 6.0793836185901715E-5 3.6476301711541026E-5 32-33 8.51113706602624E-5 0.0 0.0 6.0793836185901715E-5 5.471445256731154E-5 34-35 9.119075427885257E-5 0.0 0.0 6.0793836185901715E-5 6.687321980449189E-5 36-37 9.727013789744274E-5 0.0 0.0 6.0793836185901715E-5 9.119075427885257E-5 38-39 1.0334952151603292E-4 0.0 0.0 6.0793836185901715E-5 1.0942890513462309E-4 40-41 1.1550828875321327E-4 0.0 0.0 6.0793836185901715E-5 1.0942890513462309E-4 42-43 1.2158767237180343E-4 0.0 0.0 6.687321980449189E-5 1.0942890513462309E-4 44-45 1.2766705599039362E-4 0.0 0.0 7.295260342308205E-5 1.0942890513462309E-4 46-47 1.5806397408334446E-4 6.079383618590171E-6 0.0 7.295260342308205E-5 1.0942890513462309E-4 48-49 2.310165775064265E-4 1.2158767237180343E-5 0.0 7.295260342308205E-5 1.0942890513462309E-4 50-51 2.6749287921796756E-4 1.2158767237180343E-5 0.0 7.295260342308205E-5 1.1550828875321327E-4 52-53 2.8573103007373805E-4 1.2158767237180343E-5 0.0 7.295260342308205E-5 1.3374643960898378E-4 54-55 3.282867154038693E-4 1.2158767237180343E-5 0.0 7.295260342308205E-5 1.459052068461641E-4 56-57 3.769217843525906E-4 1.2158767237180343E-5 0.0 7.295260342308205E-5 1.5198459046475427E-4 58-59 4.6203315501285307E-4 1.2158767237180343E-5 0.0 7.295260342308205E-5 1.5806397408334446E-4 60-61 5.106682239615745E-4 1.8238150855770513E-5 0.0 7.295260342308205E-5 1.7630212493911497E-4 62-63 5.593032929102958E-4 2.4317534474360685E-5 0.0 7.295260342308205E-5 1.8238150855770513E-4 64-65 6.504940471891484E-4 2.4317534474360685E-5 0.0 7.295260342308205E-5 1.8238150855770513E-4 66-67 7.295260342308205E-4 2.4317534474360685E-5 0.0 7.295260342308205E-5 2.1885781026924618E-4 68-69 8.571930902212142E-4 2.4317534474360685E-5 0.0 7.295260342308205E-5 2.370959611250167E-4 70-71 9.362250772628864E-4 2.4317534474360685E-5 0.0 7.295260342308205E-5 2.553341119807872E-4 72-73 0.001130765353057772 2.4317534474360685E-5 0.0 7.295260342308205E-5 2.553341119807872E-4 74-75 0.001246273641810985 2.4317534474360685E-5 0.0 7.295260342308205E-5 2.553341119807872E-4 76-77 0.0013739406978013787 2.4317534474360685E-5 0.0 7.295260342308205E-5 2.553341119807872E-4 78-79 0.001592798508070625 2.4317534474360685E-5 0.0 7.295260342308205E-5 2.735722628365577E-4 80-81 0.0017994975511026908 2.4317534474360685E-5 0.0 7.295260342308205E-5 2.796516464551479E-4 82-83 0.002243292555259773 2.4317534474360685E-5 0.0 7.903198704167223E-5 2.8573103007373805E-4 84-85 0.002930262904160463 2.4317534474360685E-5 0.0 8.51113706602624E-5 2.918104136923282E-4 86-87 0.003793535378000267 2.4317534474360685E-5 0.0 8.51113706602624E-5 2.918104136923282E-4 88-89 0.005088444088759974 2.4317534474360685E-5 0.0 8.51113706602624E-5 3.039691809295086E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 375 0.0 29.082762 3 CGCGTAG 260 0.0 21.879698 54-55 CTCGCGT 255 0.0 21.379185 52-53 CAACGCA 6070 0.0 20.891449 12-13 ATCAACG 6125 0.0 20.587757 10-11 ACTCGCG 265 0.0 20.572422 50-51 TCGCGTA 325 0.0 18.962406 52-53 CGCTAGT 375 0.0 18.146643 90-91 CGTACGC 320 0.0 17.779202 20-21 ACGTACG 320 0.0 17.779202 20-21 GGTATCA 8150 0.0 17.627783 8 AGTGGTA 8350 0.0 17.376228 5 TCAACGC 6065 0.0 17.117754 10-11 AAGCAGT 8760 0.0 16.998251 1 GTATCAA 8420 0.0 16.779533 9 ACGCAGA 7865 0.0 16.183744 14-15 TGGTATC 8995 0.0 16.129948 7 AACGCAG 6460 0.0 16.107773 12-13 GTGGTAT 10020 0.0 15.189727 6 CGTAGCG 405 0.0 14.631486 56-57 >>END_MODULE