FastQCFastQC Report
Sat 18 Jun 2016
SRR578586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9740116
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127071513.046199860453408No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT327750.33649496576837484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA221290.22719441945044597No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG125020.12835576085541486No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT110280.11322247086174332No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA98160.10077908723058329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2799850.091.7131351
AGTGGTA2787200.091.69275
TGGTATC2788950.091.65367
GTGGTAT2791950.091.627266
GTATCAA2794600.091.559149
GGTATCA2795650.091.5186848
GCAGTGG2811850.091.0643543
AGCAGTG2825400.090.828582
CAGTGGT2820850.090.494464
TCAACGC2785150.046.39103312-13
CAACGCA2789850.046.3043612-13
ATCAACG2788350.046.30000710-11
AACGCAG2800050.046.1932914-15
CGCAGAG2805950.046.11807316-17
ACGCAGA2802350.046.1121514-15
TATCAAC2800700.046.0110210-11
AGAGTAC2730900.045.86886218-19
CAGAGTA2797050.045.70786718-19
GAGTACT1656250.045.69714420-21
GCAGAGT2831250.045.42913416-17
Produced by FastQC (version 0.11.5)