##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578586_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9740116 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.66886431332029 31.0 31.0 34.0 27.0 34.0 2 30.97082950552129 31.0 31.0 34.0 27.0 34.0 3 31.04718362697118 31.0 31.0 34.0 27.0 34.0 4 34.451025531934114 37.0 35.0 37.0 30.0 37.0 5 34.551674333242026 37.0 35.0 37.0 30.0 37.0 6 34.53426920172203 37.0 35.0 37.0 30.0 37.0 7 34.515376408248116 37.0 35.0 37.0 31.0 37.0 8 34.471137920739345 37.0 35.0 37.0 30.0 37.0 9 35.873390932921126 39.0 35.0 39.0 30.0 39.0 10-11 35.84285110156799 39.0 35.0 39.0 30.0 39.0 12-13 35.90164583255476 39.0 35.0 39.0 30.0 39.0 14-15 36.97743491966625 40.0 37.0 41.0 31.0 41.0 16-17 36.92569349276744 40.0 36.5 41.0 30.5 41.0 18-19 36.9331782598893 40.0 36.5 41.0 30.5 41.0 20-21 36.814134503120904 39.5 36.0 41.0 30.0 41.0 22-23 36.50664899678813 39.5 36.0 40.5 29.5 41.0 24-25 36.41223944355488 39.0 36.0 40.5 30.0 41.0 26-27 36.33533579066204 39.0 36.0 41.0 29.0 41.0 28-29 36.07737628586764 39.0 35.5 41.0 27.0 41.0 30-31 35.8406929137189 39.0 35.0 41.0 27.0 41.0 32-33 35.48807462867999 38.5 34.0 40.0 25.5 41.0 34-35 35.28664607279831 38.0 34.0 40.0 25.0 41.0 36-37 34.94285601937389 38.0 34.0 40.0 25.0 41.0 38-39 34.196558439345075 37.0 33.0 40.0 25.0 41.0 40-41 34.33798817180411 37.0 33.0 40.0 25.0 41.0 42-43 34.24463302079769 37.0 33.0 40.0 24.0 41.0 44-45 34.090651795112095 37.0 33.0 40.0 23.0 41.0 46-47 33.858564056115966 37.0 33.0 40.0 23.0 41.0 48-49 33.47939957799271 37.0 33.0 40.0 20.5 41.0 50-51 32.21961047486498 36.0 31.5 39.0 15.0 40.5 52-53 31.781518156457274 36.5 31.0 39.0 5.0 40.0 54-55 31.327983516828752 37.0 30.5 40.0 2.0 41.0 56-57 30.80958661067281 37.0 29.0 40.0 2.0 41.0 58-59 30.472999602879472 36.0 28.0 40.0 2.0 41.0 60-61 30.176401595217143 36.0 28.0 40.0 2.0 41.0 62-63 29.84692666904583 35.0 28.0 39.0 2.0 41.0 64-65 29.515290064307244 35.0 27.0 39.0 2.0 41.0 66-67 29.20311749880597 35.0 26.5 39.0 2.0 40.5 68-69 28.8771294407582 35.0 26.0 38.5 2.0 40.0 70-71 28.50389117542337 35.0 26.0 37.5 2.0 40.0 72-73 28.107402622309632 34.5 26.0 37.0 2.0 39.5 74-75 27.738384635254857 34.0 25.0 36.5 2.0 39.0 76-77 27.350838275437376 34.0 24.5 36.0 2.0 39.0 78-79 26.998515777430164 34.0 23.5 36.0 2.0 38.5 80-81 26.632651654251347 34.0 22.5 35.0 2.0 37.0 82-83 26.246854144242224 34.0 20.5 35.0 2.0 37.0 84-85 25.891718332718007 33.0 20.0 35.0 2.0 36.5 86-87 25.536414607382497 33.0 18.0 35.0 2.0 36.0 88-89 25.18914148455727 33.0 16.0 35.0 2.0 36.0 90-91 24.823849787825935 32.5 9.0 35.0 2.0 35.0 92-93 24.466694749836655 32.0 4.5 35.0 2.0 35.0 94-95 24.03499691379446 32.0 2.0 35.0 2.0 35.0 96-97 23.560060526999884 32.0 2.0 35.0 2.0 35.0 98-99 23.061344187276617 32.0 2.0 35.0 2.0 35.0 100-101 21.762386454124368 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.605068083294455 1101 2 0.6903798715334553 1101 3 0.6965597414015008 1101 4 0.6881224288524947 1101 5 0.6564351251065332 1101 6 0.5055919459480052 1101 7 0.5866569463975964 1101 8 0.6386272975441187 1101 9 0.8646581297243827 1101 10-11 0.8429487527122177 1101 12-13 0.8766119877260152 1101 14-15 0.9133951472581927 1101 16-17 0.9133994044494997 1101 18-19 0.9526261613200475 1101 20-21 0.9040323673884529 1101 22-23 0.888284085103983 1101 24-25 0.9006088073292986 1101 26-27 1.1107279802803802 1101 28-29 1.1503505529718367 1101 30-31 1.2263284176701532 1101 32-33 1.0940394504963749 1101 34-35 1.2723531527502132 1101 36-37 1.198623807057139 1101 38-39 1.1674582820668675 1101 40-41 1.2578513919183791 1101 42-43 1.459448935302575 1101 44-45 1.453527919234439 1101 46-47 1.5315302565670308 1101 48-49 1.4241280265682406 1101 50-51 1.3235440191791454 1101 52-53 1.3077371866979846 1101 54-55 1.3149103755481555 1101 56-57 1.2311389685281533 1101 58-59 1.1930182712646449 1101 60-61 1.2446195720205075 1101 62-63 1.289076267119004 1101 64-65 1.1132434341414168 1101 66-67 1.2188417844192578 1101 68-69 1.231756788035355 1101 70-71 1.2136912313961474 1101 72-73 1.283342172746142 1101 74-75 1.2186705345568143 1101 76-77 1.2822486772256845 1101 78-79 1.2855193836659744 1101 80-81 1.2953747575725707 1101 82-83 1.2495385695853862 1101 84-85 1.2446463541575028 1101 86-87 1.2885158757274233 1101 88-89 1.2714249988542505 1101 90-91 1.3390076766055437 1101 92-93 1.473171718385057 1101 94-95 1.5924508503463635 1101 96-97 1.70344015168007 1101 98-99 1.8145403445369936 1101 100-101 2.1316070654064063 1102 1 0.4921914692819307 1102 2 0.4883611733948534 1102 3 0.5574989691241115 1102 4 0.6071332763721173 1102 5 0.4749847883998868 1102 6 0.43332581525175584 1102 7 0.3482103517919555 1102 8 0.5613209130766208 1102 9 0.6118849531210486 1102 10-11 0.6990715724071208 1102 12-13 0.5858437510166894 1102 14-15 0.6546946755835847 1102 16-17 0.6141016111274311 1102 18-19 0.6219756662059837 1102 20-21 0.7457122273703689 1102 22-23 0.7325401916395649 1102 24-25 0.6799522773222932 1102 26-27 0.8587660882938977 1102 28-29 0.7869229421665622 1102 30-31 0.9441923369204019 1102 32-33 0.9506068944090842 1102 34-35 0.951576587025265 1102 36-37 0.9519559569763061 1102 38-39 0.9807455011970987 1102 40-41 1.0228944571908727 1102 42-43 1.1381225006960918 1102 44-45 0.9692209129064864 1102 46-47 1.0998586626324638 1102 48-49 1.0857219815873975 1102 50-51 1.1189296484479385 1102 52-53 1.103707220028653 1102 54-55 1.0813872025882212 1102 56-57 1.1301299602665544 1102 58-59 0.9608260822071237 1102 60-61 1.1477959095276304 1102 62-63 1.2351388881645633 1102 64-65 1.1061569709356505 1102 66-67 1.1487671608405527 1102 68-69 1.1420299590397072 1102 70-71 1.0998956537623137 1102 72-73 1.1984732654019226 1102 74-75 1.204174441300399 1102 76-77 1.1229967202672917 1102 78-79 1.0818789439695102 1102 80-81 1.1309111530273874 1102 82-83 1.0705808517142472 1102 84-85 1.0589094517950493 1102 86-87 1.137248475181682 1102 88-89 1.2076858443695926 1102 90-91 1.2678191311718372 1102 92-93 1.4475208267980335 1102 94-95 1.4564174079578116 1102 96-97 1.6209898592135055 1102 98-99 1.7266754291545183 1102 100-101 2.030898779368254 1103 1 0.44813790088108263 1103 2 0.553099705002758 1103 3 0.48426877655087 1103 4 0.5912356793848659 1103 5 0.5220250228902685 1103 6 0.5062706044157039 1103 7 0.41303419865180047 1103 8 0.4815425391113166 1103 9 0.5466910924277215 1103 10-11 0.5780350131373453 1103 12-13 0.5913358827862325 1103 14-15 0.7200486254600094 1103 16-17 0.6048457562322227 1103 18-19 0.6628959643472925 1103 20-21 0.7231265717799218 1103 22-23 0.6050329975768349 1103 24-25 0.6497213863269522 1103 26-27 0.8246848373689417 1103 28-29 0.7451792134698536 1103 30-31 0.8724225733380777 1103 32-33 0.8431976664270096 1103 34-35 0.9038944669482021 1103 36-37 0.9250104857464052 1103 38-39 0.9391196445748591 1103 40-41 0.892636356735224 1103 42-43 1.0490693088598277 1103 44-45 0.9228351006079549 1103 46-47 0.9134543367895205 1103 48-49 1.071258646861203 1103 50-51 1.071446274246 1103 52-53 0.9427243723641254 1103 54-55 0.9305407676991813 1103 56-57 0.810997983407372 1103 58-59 0.8188830313349591 1103 60-61 0.7838422730549333 1103 62-63 0.8366136531444184 1103 64-65 0.8904465813788107 1103 66-67 0.8783702441468186 1103 68-69 0.8002350753967136 1103 70-71 0.7735321391039562 1103 72-73 0.85356711145349 1103 74-75 0.8475651202479035 1103 76-77 0.917508157206445 1103 78-79 0.9310850031994597 1103 80-81 0.9479371875191269 1103 82-83 0.9628692992745549 1103 84-85 1.0406892401008676 1103 86-87 1.055429277676577 1103 88-89 1.1309694234981293 1103 90-91 1.1250216943318172 1103 92-93 1.1237275597811127 1103 94-95 1.2637621670048809 1103 96-97 1.3620367122851356 1103 98-99 1.427686428882808 1103 100-101 1.671122454793256 1104 1 0.3412376107988955 1104 2 0.27266099148271294 1104 3 0.40766069418847906 1104 4 0.4587760517863657 1104 5 0.3604204203608532 1104 6 0.35335398118959915 1104 7 0.35180996369617645 1104 8 0.3452788540269651 1104 9 0.44155861656578566 1104 10-11 0.5470235371326169 1104 12-13 0.5127817501211567 1104 14-15 0.49175880398601635 1104 16-17 0.6316767612818026 1104 18-19 0.5303760507195605 1104 20-21 0.6295817518547224 1104 22-23 0.5647618798208072 1104 24-25 0.575473259398116 1104 26-27 0.5534061846528928 1104 28-29 0.5726969006032263 1104 30-31 0.7026136208798235 1104 32-33 0.7361721128056686 1104 34-35 0.7911563323608846 1104 36-37 0.9424192344879216 1104 38-39 0.8509137045655777 1104 40-41 0.8630084656956285 1104 42-43 0.9012711413019616 1104 44-45 0.888382443605451 1104 46-47 0.8750704249075412 1104 48-49 0.8798889199473692 1104 50-51 0.6748904499555479 1104 52-53 0.7122709695869673 1104 54-55 0.7749828609337932 1104 56-57 0.6746362493903 1104 58-59 0.5880797716821178 1104 60-61 0.5721942135508051 1104 62-63 0.6979718394175798 1104 64-65 0.7266347275963696 1104 66-67 0.5371854097931568 1104 68-69 0.5538716309481551 1104 70-71 0.6205924452452756 1104 72-73 0.658686695287205 1104 74-75 0.6972135565572941 1104 76-77 0.7462779071095405 1104 78-79 0.7224032956121995 1104 80-81 0.7092681330662671 1104 82-83 0.6408954555150892 1104 84-85 0.7384503409428476 1104 86-87 0.790577104625914 1104 88-89 0.8084631217947695 1104 90-91 0.7727816670083101 1104 92-93 0.9118764003514777 1104 94-95 0.9045432055146669 1104 96-97 0.8055014917321976 1104 98-99 0.9390278122210418 1104 100-101 1.261048063581189 1105 1 -0.012696572666243355 1105 2 0.0585355511587764 1105 3 0.19946287311814714 1105 4 0.17275083654741508 1105 5 0.25390381596739076 1105 6 0.1941325004369645 1105 7 0.22014774387962177 1105 8 0.2861715095559987 1105 9 0.2278664093128384 1105 10-11 0.15275363521411123 1105 12-13 0.15556019002496413 1105 14-15 0.041252004765034656 1105 16-17 0.16444175336413736 1105 18-19 0.1267256467402902 1105 20-21 0.34651898844360574 1105 22-23 0.31067179097075126 1105 24-25 0.3999717788911781 1105 26-27 0.5515392962304304 1105 28-29 0.524753705020423 1105 30-31 0.4529911866977727 1105 32-33 0.5227558393654377 1105 34-35 0.5480997480874947 1105 36-37 0.5221315392300383 1105 38-39 0.6004069374106962 1105 40-41 0.5131556556143977 1105 42-43 0.5897731061372866 1105 44-45 0.5848049960192157 1105 46-47 0.656688415048599 1105 48-49 0.7567697809229941 1105 50-51 0.6787967674775572 1105 52-53 0.4280228276246447 1105 54-55 0.49304607362105557 1105 56-57 0.5107130665018609 1105 58-59 0.6614904612116206 1105 60-61 0.5738563999985722 1105 62-63 0.5996287439772097 1105 64-65 0.6693437724485207 1105 66-67 0.7664524531690411 1105 68-69 0.6684182785529948 1105 70-71 0.5201192538037063 1105 72-73 0.6777833613455861 1105 74-75 0.5411091112927835 1105 76-77 0.541839054815032 1105 78-79 0.6398997920649521 1105 80-81 0.5962889566622387 1105 82-83 0.6524125920555228 1105 84-85 0.6032886773378756 1105 86-87 0.7352248609572918 1105 88-89 0.7776759868869974 1105 90-91 0.8044568525525442 1105 92-93 0.8584437287078224 1105 94-95 0.9545407559651373 1105 96-97 1.016466973085116 1105 98-99 1.0749817339949246 1105 100-101 1.2705249092456121 1106 1 0.190056119061218 1106 2 0.3006286241966549 1106 3 0.3407750973685211 1106 4 0.4942458773503233 1106 5 0.3707003699196889 1106 6 0.37283908984943537 1106 7 0.36456050927698413 1106 8 0.24130118889377883 1106 9 0.1391164028202212 1106 10-11 0.2921291816297753 1106 12-13 0.3453964468398638 1106 14-15 0.3200810089703694 1106 16-17 0.32311312904581513 1106 18-19 0.23680419577190293 1106 20-21 0.3357639587118513 1106 22-23 0.3449573817981175 1106 24-25 0.338435386488392 1106 26-27 0.2965883046949216 1106 28-29 0.2727863323146167 1106 30-31 0.45615427796912655 1106 32-33 0.4264013512337712 1106 34-35 0.4056700758114715 1106 36-37 0.4341331840993874 1106 38-39 0.6585257280537675 1106 40-41 0.43520046338494467 1106 42-43 0.36443606208597856 1106 44-45 0.4581692854017305 1106 46-47 0.4637492410660897 1106 48-49 0.43126794050161976 1106 50-51 0.5765195230028226 1106 52-53 0.5626606441699948 1106 54-55 0.4516477821677576 1106 56-57 0.379278623065904 1106 58-59 0.5704788146215911 1106 60-61 0.4935477869680085 1106 62-63 0.46426309153244105 1106 64-65 0.5583110024825686 1106 66-67 0.614647832350439 1106 68-69 0.6823420356980456 1106 70-71 0.5664155736350907 1106 72-73 0.6436626552983462 1106 74-75 0.6962664077261067 1106 76-77 0.5806993694465739 1106 78-79 0.5717479504224166 1106 80-81 0.5518039933916477 1106 82-83 0.5247252436580716 1106 84-85 0.6051915383208879 1106 86-87 0.7182164212215199 1106 88-89 0.7296983838099749 1106 90-91 0.7256288871655272 1106 92-93 0.7453274649350554 1106 94-95 0.685453436323062 1106 96-97 0.8542846677638138 1106 98-99 0.9794517250201977 1106 100-101 1.247320415538585 1107 1 0.1346708569842292 1107 2 0.09918085078646399 1107 3 0.22500927959719874 1107 4 0.44328619142703474 1107 5 0.1701957759948698 1107 6 0.23473193200187126 1107 7 0.23753784758373087 1107 8 0.2426445973364295 1107 9 0.16111582934514956 1107 10-11 0.17246963927617998 1107 12-13 0.12621341486300253 1107 14-15 0.2726058786508432 1107 16-17 0.2691173954886281 1107 18-19 0.34039206247361165 1107 20-21 0.28164471666027424 1107 22-23 0.30151854790685917 1107 24-25 0.4587250820083639 1107 26-27 0.3004400435372574 1107 28-29 0.25131074466784753 1107 30-31 0.3851294122877462 1107 32-33 0.4926724197872261 1107 34-35 0.24783596659441542 1107 36-37 0.364506695192226 1107 38-39 0.549498952385882 1107 40-41 0.38184077056078536 1107 42-43 0.3656710600928079 1107 44-45 0.5809615783093989 1107 46-47 0.4933589729635983 1107 48-49 0.563492091050847 1107 50-51 0.36690887266424 1107 52-53 0.6087823538026669 1107 54-55 0.5446921713099648 1107 56-57 0.5066547609311378 1107 58-59 0.7370386550377184 1107 60-61 0.7443167205613186 1107 62-63 0.548512615243272 1107 64-65 0.7598056252747227 1107 66-67 0.8414215847262803 1107 68-69 0.7773087757279491 1107 70-71 0.6536866023520815 1107 72-73 0.582921634663407 1107 74-75 0.7192163309336941 1107 76-77 0.6842922784941337 1107 78-79 0.5918775649851149 1107 80-81 0.5225071139391453 1107 82-83 0.5478317693770656 1107 84-85 0.5969640382243853 1107 86-87 0.7487823973031844 1107 88-89 0.7279550362000435 1107 90-91 0.6692539101603607 1107 92-93 0.7805970320286058 1107 94-95 0.6855409142618356 1107 96-97 0.7672428001422773 1107 98-99 0.733800400569816 1107 100-101 0.8561185758821672 1108 1 0.20663286543049608 1108 2 0.15670549803559197 1108 3 0.20285264256319735 1108 4 0.47750122067194667 1108 5 0.25640126065799507 1108 6 0.3722798487865262 1108 7 0.3480488371932182 1108 8 0.24543835463875752 1108 9 0.3331851472078853 1108 10-11 0.3197397784375866 1108 12-13 0.15743410286790294 1108 14-15 0.3676477517242134 1108 16-17 0.3157774974551941 1108 18-19 0.3106670499926949 1108 20-21 0.3139749838089472 1108 22-23 0.2612699044897724 1108 24-25 0.49074654808256213 1108 26-27 0.452782211372174 1108 28-29 0.31959889188772905 1108 30-31 0.37957906414779785 1108 32-33 0.6348073653653543 1108 34-35 0.4645466637232474 1108 36-37 0.45752896473334204 1108 38-39 0.48118250728641954 1108 40-41 0.47571129500357046 1108 42-43 0.4646676962797258 1108 44-45 0.48299244165322364 1108 46-47 0.564161904156613 1108 48-49 0.6441027270258459 1108 50-51 0.46647858263619213 1108 52-53 0.5162446066380255 1108 54-55 0.387026594686521 1108 56-57 0.2837459937028548 1108 58-59 0.42145954301183153 1108 60-61 0.3487487702501717 1108 62-63 0.24134063449072585 1108 64-65 0.1484589488421335 1108 66-67 0.3098226397439241 1108 68-69 0.4211440696815387 1108 70-71 0.3297460881295393 1108 72-73 0.29011412396111425 1108 74-75 0.3055322950804644 1108 76-77 0.34993868235843095 1108 78-79 0.30813199125747204 1108 80-81 0.3930787322192195 1108 82-83 0.35429501898020277 1108 84-85 0.3163561958768817 1108 86-87 0.3101553521926199 1108 88-89 0.334589985876363 1108 90-91 0.291829796227983 1108 92-93 0.3271105867685584 1108 94-95 0.5106221705289542 1108 96-97 0.5529882467705285 1108 98-99 0.4725449004244524 1108 100-101 0.756102089493023 1109 1 -0.27071347689449254 1109 2 0.10732171822672498 1109 3 0.11347156538883496 1109 4 0.18041081009903337 1109 5 0.0712912370716623 1109 6 0.1423084334398368 1109 7 0.14641376922618576 1109 8 0.08992760117825327 1109 9 0.19712755126105463 1109 10-11 0.22071316082806902 1109 12-13 0.13181612019968014 1109 14-15 0.08398466838928442 1109 16-17 0.00320794391907242 1109 18-19 0.08966750123678935 1109 20-21 0.21203125958433588 1109 22-23 0.11316595924947137 1109 24-25 0.11649500170843652 1109 26-27 0.14104484126816175 1109 28-29 -0.05044973767494554 1109 30-31 0.03235626862124974 1109 32-33 0.3215896347986771 1109 34-35 0.2009137866408537 1109 36-37 0.2206812192415626 1109 38-39 0.28440431169893543 1109 40-41 0.22942899740854727 1109 42-43 0.2241820722401826 1109 44-45 0.0958379067092352 1109 46-47 0.22302148437089642 1109 48-49 0.32794826409844546 1109 50-51 0.1873691210623747 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90-91 0.24454742098785331 2311 92-93 0.1292396207276596 2311 94-95 0.09046200624777256 2311 96-97 0.18921690623603382 2311 98-99 0.2644249568965691 2311 100-101 0.2427859533300385 2312 1 0.05216819429806563 2312 2 -0.2094113236655133 2312 3 0.041029957912041226 2312 4 0.13443936382740418 2312 5 0.1481442566531399 2312 6 0.061737221960740385 2312 7 0.03116936225659117 2312 8 -0.03325470609762249 2312 9 0.09241258137837605 2312 10-11 -1.3922684224354498E-4 2312 12-13 -0.009695339538389192 2312 14-15 0.03001775958888686 2312 16-17 -0.04960536555110906 2312 18-19 -0.02085618738620809 2312 20-21 0.03740716444599457 2312 22-23 0.07529250407955601 2312 24-25 0.0023263187210389447 2312 26-27 0.01838003722064485 2312 28-29 -0.0724503951612192 2312 30-31 -0.10043423830445875 2312 32-33 -0.0983169616987567 2312 34-35 -0.07030344639601083 2312 36-37 -0.05749866958124272 2312 38-39 -0.047147020637183346 2312 40-41 -0.04140892645840211 2312 42-43 0.0019625167527124177 2312 44-45 0.07470839068636081 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0.06708991139351639 2313 5 -0.42053392386428357 2313 6 -0.3037241204811352 2313 7 -0.3229313589617817 2313 8 -0.200262577738755 2313 9 -0.022153809149450865 2313 10-11 -0.1908519415800356 2313 12-13 -0.22613991689878077 2313 14-15 -0.5401889653199348 2313 16-17 -0.3505587402069281 2313 18-19 -0.24337780719859126 2313 20-21 -0.24770357531589582 2313 22-23 -0.2631360017224438 2313 24-25 -0.5418016869059912 2313 26-27 -0.38071346223406266 2313 28-29 -0.4586640884330322 2313 30-31 -0.5778881145047947 2313 32-33 -0.9834132252509491 2313 34-35 -0.6305021760586769 2313 36-37 -0.33507585770318116 2313 38-39 -0.3516115008275591 2313 40-41 -0.1897217757921723 2313 42-43 -0.2683561832765662 2313 44-45 -0.2592109785461929 2313 46-47 -0.37350852924497957 2313 48-49 -0.3647587229818612 2313 50-51 -0.26982903129513147 2313 52-53 -0.2354899933418224 2313 54-55 -0.16038399914764412 2313 56-57 -0.15149849515698932 2313 58-59 -0.1924349518538797 2313 60-61 -0.33222173150534573 2313 62-63 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2315 40-41 -2.5731611488048713 2315 42-43 -2.100962641102022 2315 44-45 -1.9077813731559274 2315 46-47 -1.475415833652697 2315 48-49 -1.9225613930153145 2315 50-51 -1.640366197371165 2315 52-53 -1.4460522924512489 2315 54-55 -1.4092722496763166 2315 56-57 -1.507745305281901 2315 58-59 -1.5607333518548465 2315 60-61 -1.2324231714283833 2315 62-63 -1.1325768261806992 2315 64-65 -1.0773301903253554 2315 66-67 -1.0454782566580647 2315 68-69 -1.2939397191610205 2315 70-71 -1.0210878891091113 2315 72-73 -1.0292188693342545 2315 74-75 -1.1098858928336526 2315 76-77 -0.9749122758976547 2315 78-79 -1.070336675489397 2315 80-81 -1.17557740201568 2315 82-83 -1.0688593012171097 2315 84-85 -1.235610759206935 2315 86-87 -1.3547202419092201 2315 88-89 -1.2817483388707487 2315 90-91 -1.2151854251907288 2315 92-93 -1.239813351141546 2315 94-95 -1.187507998644886 2315 96-97 -1.3198722392554991 2315 98-99 -1.4249658366035227 2315 100-101 -1.7203494849593532 2316 1 -0.8698718894479178 2316 2 -1.0915815433052671 2316 3 -1.3306810422463649 2316 4 -1.253352267884054 2316 5 -0.848205523337505 2316 6 -0.7937999460525305 2316 7 -1.0126150003424712 2316 8 -1.082523739937237 2316 9 -1.3985138021364207 2316 10-11 -1.3129744273226294 2316 12-13 -1.2528921334674763 2316 14-15 -1.2493503755529431 2316 16-17 -2.0499518928438576 2316 18-19 -1.3900356185244718 2316 20-21 -1.7694356208158268 2316 22-23 -1.31456748225483 2316 24-25 -1.2474362576713176 2316 26-27 -1.3497609808430937 2316 28-29 -1.6288138466580904 2316 30-31 -1.475556502535305 2316 32-33 -1.6377602538643075 2316 34-35 -2.049554428752522 2316 36-37 -1.7027466385593115 2316 38-39 -1.7736895212667747 2316 40-41 -2.143341370376895 2316 42-43 -2.141312612793996 2316 44-45 -2.38109094091989 2316 46-47 -2.208288826170211 2316 48-49 -2.903184523042192 2316 50-51 -2.369325438547321 2316 52-53 -3.0067469651948606 2316 54-55 -4.164088474225732 2316 56-57 -2.9835054312716274 2316 58-59 -2.4395982610206985 2316 60-61 -2.1502434577317153 2316 62-63 -2.762545509849442 2316 64-65 -2.7831546613869236 2316 66-67 -2.5004281433748723 2316 68-69 -2.5577488602513974 2316 70-71 -2.7463307582934178 2316 72-73 -5.719802679801333 2316 74-75 -3.566715280775149 2316 76-77 -2.953822641781752 2316 78-79 -2.869995874053071 2316 80-81 -2.816018105476232 2316 82-83 -2.8699118027899573 2316 84-85 -2.913018522338618 2316 86-87 -2.720451076597211 2316 88-89 -2.4543248661954777 2316 90-91 -2.54293338798378 2316 92-93 -2.9004061450869507 2316 94-95 -3.220455868620771 2316 96-97 -3.2247794661829836 2316 98-99 -3.6154995559281886 2316 100-101 -3.6392831448331897 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96517.0 3 17069.0 4 14715.0 5 14326.0 6 16226.0 7 20256.0 8 25555.0 9 30407.0 10 35335.0 11 42312.0 12 45154.0 13 50980.0 14 58732.0 15 70293.0 16 90106.0 17 121899.0 18 176619.0 19 257923.0 20 333870.0 21 240819.0 22 140040.0 23 122234.0 24 125737.0 25 134849.0 26 148721.0 27 167690.0 28 191989.0 29 223886.0 30 263490.0 31 313400.0 32 379339.0 33 469505.0 34 603907.0 35 831709.0 36 1202449.0 37 1369181.0 38 1102563.0 39 190073.0 40 241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.027438576651198 48.96444269187246 24.286115529367006 16.722003202109335 2 15.71175904061615 50.59882371472817 18.579908700666028 15.109508543989655 3 44.356592222055525 20.77729093408908 19.447954194676733 15.418162649178658 4 15.733895298700634 19.296828009856064 20.378195066186297 44.591081625257004 5 15.707520868182993 48.07116167785503 21.399464639393955 14.821852814568024 6 44.609504329477886 20.319284157638805 19.253092230965912 15.818119281917395 7 16.555895355641475 19.74883660320405 48.93948527926148 14.755782761892997 8 44.18616551925826 20.519417023295457 20.07780599387092 15.216611463575362 9 43.76498611228703 20.141078038343075 20.703491368574724 15.390444480795171 10-11 15.419305553272288 34.338642958128524 34.51724814644331 15.72480334215588 12-13 15.30942199125247 20.440883070946107 34.463336593974006 29.78635834382742 14-15 15.473301404179635 48.70290017866072 20.12497362626286 15.698824790896785 16-17 29.660755187032763 20.282598105906715 20.261932524178807 29.794714182881716 18-19 15.612444977354025 34.38140158803814 20.23654049180838 29.769612942799462 20-21 29.84244798086879 34.39995665072399 20.2426532222277 15.51494214617951 22-23 29.744873554297683 20.19089982897211 34.44269158518336 15.621535031546847 24-25 15.59752695859695 34.15851075177671 20.690426247481735 29.553536042144607 26-27 15.818579895155633 25.441283256511866 42.94109849775596 15.799038350576541 28-29 26.439624835516568 20.114735507149245 37.72141901683717 15.72422064049702 30-31 24.01262515337505 21.29777247096645 38.18838271855104 16.50121965710746 32-33 18.487830948295507 23.207403466090533 39.8648375570805 18.439928028533462 34-35 18.024519611009865 22.56718738179057 40.737314043489306 18.670978963710255 36-37 17.963684363870854 22.637540517064327 40.59480511570035 18.80397000336447 38-39 18.51251033173677 22.961922558442332 40.02869594465882 18.49687116516208 40-41 18.54096373315014 23.11876090865165 39.88695575894633 18.453319599251884 42-43 18.909489298363415 22.987397652340125 39.85084279118001 18.25227025811645 44-45 18.524090313618828 22.888218276144954 40.031357311827755 18.55633409840846 46-47 18.722373907307595 22.914745495343276 39.77859478416494 18.584285813184188 48-49 18.857404882854567 23.128225901788895 39.511798764840314 18.50257045051622 50-51 18.972811458169208 23.517825738357534 38.814400280365405 18.694962523107858 52-53 19.12632016542333 24.05649207740263 37.80251755491665 19.014670202257392 54-55 19.90419777707043 24.813645715630958 36.151817024245645 19.130339483052964 56-57 20.209028849506286 25.627369775004162 34.745326493375195 19.418274882114357 58-59 20.42950385979275 26.397582406915447 33.85743278136452 19.315480951927277 60-61 20.508772248790162 26.571676385305327 33.329882017380456 19.589669348524055 62-63 20.03945358510366 26.604986027968103 33.52616365502828 19.829396731899955 64-65 20.194791457858997 26.277212149207614 33.69576053087852 19.832235862054866 66-67 19.96667998508611 25.931902476301598 33.87722129153061 20.224196247081675 68-69 20.051499405726705 26.028112313466927 34.01263095877546 19.907757322030903 70-71 19.70356268487784 25.88334711268662 34.5090442189608 19.90404598347474 72-73 19.71395246773167 25.572209585026016 34.544232588957 20.16960535828531 74-75 20.072308620868878 25.550619321550467 34.033308229311885 20.34376382826877 76-77 20.10968702625588 25.836914773385004 34.01403034982198 20.039367850537136 78-79 20.25476176756467 26.206487181280735 33.10128585211391 20.437465199040687 80-81 20.30160096575416 26.265598212619366 33.07581155796882 20.356989263657645 82-83 20.66376439135287 26.469751280425395 32.61716953536359 20.249314792858144 84-85 20.68742044184748 26.603994554943235 32.57150437411275 20.137080629096545 86-87 20.751478348457074 26.613533535544715 32.39174485332176 20.24324326267645 88-89 20.72223686424218 26.571354648873292 32.17622060180825 20.53018788507628 90-91 20.95191150137336 26.697742386480318 32.09831108201343 20.25203503013289 92-93 20.871309771726164 26.851536731385877 31.998065351493928 20.279088145394034 94-95 20.680422832016813 26.991426564262095 31.69705074406354 20.631099859657553 96-97 20.554755500120798 26.779432119539837 32.16400346199064 20.50180891834873 98-99 20.604316599962697 26.787465418969674 31.887284122757215 20.720933858310417 100-101 20.91782901474457 26.58786601801615 31.539608750671682 20.954696216567598 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10966.0 1 6861.5 2 1996.5 3 1127.5 4 946.5 5 825.5 6 905.0 7 1108.0 8 1110.0 9 1170.5 10 1646.0 11 11012.0 12 189259.5 13 211406.5 14 55380.5 15 47685.0 16 51301.5 17 56319.5 18 60804.0 19 64648.0 20 68754.0 21 73207.5 22 78293.0 23 84368.5 24 91743.5 25 100588.5 26 112634.5 27 125974.5 28 137447.5 29 148850.5 30 159774.5 31 171251.5 32 185970.0 33 202095.0 34 225044.5 35 238306.0 36 243754.5 37 255719.5 38 263831.5 39 272367.0 40 283303.0 41 288724.5 42 290518.0 43 290918.5 44 295496.5 45 299615.5 46 292716.5 47 289056.0 48 288084.0 49 283637.0 50 277996.5 51 268523.5 52 262334.5 53 256596.0 54 250150.5 55 242372.5 56 228860.0 57 212727.5 58 204715.5 59 173617.0 60 120947.5 61 88062.5 62 65568.5 63 48060.0 64 34813.5 65 24756.0 66 16341.5 67 10443.0 68 6736.5 69 4581.5 70 3411.5 71 2773.0 72 2419.5 73 2154.0 74 1898.0 75 1730.5 76 1648.5 77 1548.5 78 1463.0 79 1371.5 80 1241.5 81 1125.5 82 1015.0 83 927.0 84 821.5 85 684.5 86 599.5 87 504.5 88 385.0 89 305.0 90 259.5 91 186.5 92 125.0 93 95.0 94 60.5 95 42.5 96 30.5 97 21.0 98 14.5 99 5.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0991261295040018 2 0.08165200496585462 3 0.08722688723625058 4 0.1950182112820833 5 0.07430096315074687 6 0.09276070223393643 7 0.09813024814078189 8 0.08701128405452256 9 0.07979371087572262 10-11 0.0767547326951753 12-13 0.0703790386069324 14-15 0.07620545792267772 16-17 0.06715012429010085 18-19 0.05867486588455415 20-21 0.053757059977519776 22-23 0.043911181345273505 24-25 0.046123680662530094 26-27 0.04566167384454148 28-29 0.044486123163214895 30-31 0.034527309531015855 32-33 0.03857756930204938 34-35 0.05144189247848793 36-37 0.04679102384406921 38-39 0.050743748842416246 40-41 0.04850044907062708 42-43 0.04670375588956025 44-45 0.05037414338802536 46-47 0.044167851799711624 48-49 0.03988658861968379 50-51 0.04363397725448034 52-53 0.0398711883924175 54-55 0.04337730680004222 56-57 0.04200668657334266 58-59 0.04941932929751555 60-61 0.04824377861618897 62-63 0.05269444429614596 64-65 0.04909079111583476 66-67 0.04338757361821974 68-69 0.04389578111800722 70-71 0.052776578841566155 72-73 0.039701785892488343 74-75 0.0474737672528746 76-77 0.046272549526104205 78-79 0.04510213225386638 80-81 0.0441986522542442 82-83 0.04303336839109514 84-85 0.0442807867996644 86-87 0.048859787706840456 88-89 0.04166274816439558 90-91 0.044419388845060984 92-93 0.040867069755637406 94-95 0.04401898293613751 96-97 0.030220379305544203 98-99 0.03659093998469833 100-101 0.025292306580332307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9740116.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.9777270400493 #Duplication Level Percentage of deduplicated Percentage of total 1 89.79791269938787 35.899164426623045 2 5.408041979743965 4.324024521746642 3 1.6473848002726394 1.9757609962567715 4 0.8478958788177562 1.355878000070359 5 0.524409957442875 1.0482359067867562 6 0.3274670227319987 0.7854832349638476 7 0.22678003410934564 0.6346305211229543 8 0.1588253171970345 0.5079580142361835 9 0.12141905648148323 0.43686521077293583 >10 0.8363468468612767 6.812490268848849 >50 0.06435162077914627 1.681199559562813 >100 0.02759876524809419 2.0330118846394614 >500 0.005140453745117076 1.5087821594188466 >1k 0.005590243447813268 4.796609474752501 >5k 5.14045374511565E-4 1.5430242062135604 >10k+ 3.212783590697281E-4 34.65688161398447 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1270715 13.046199860453408 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 32775 0.33649496576837484 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA 22129 0.22719441945044597 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 12502 0.12835576085541486 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 11028 0.11322247086174332 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA 9816 0.10077908723058329 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0266818177524786E-5 0.0 0.0 0.0 0.0 2 1.0266818177524786E-5 0.0 0.0 0.0 0.0 3 3.0800454532574356E-5 0.0 0.0 0.0 0.0 4 4.1067272710099144E-5 0.0 0.0 3.0800454532574356E-5 0.0 5 4.1067272710099144E-5 0.0 0.0 3.0800454532574356E-5 0.0 6 5.1334090887623924E-5 0.0 0.0 3.0800454532574356E-5 1.0266818177524786E-5 7 6.160090906514871E-5 0.0 0.0 4.1067272710099144E-5 1.0266818177524786E-5 8 8.213454542019829E-5 0.0 0.0 4.1067272710099144E-5 1.0266818177524786E-5 9 9.240136359772307E-5 0.0 0.0 6.160090906514871E-5 1.0266818177524786E-5 10-11 1.0780159086401023E-4 0.0 0.0 6.160090906514871E-5 1.0266818177524786E-5 12-13 1.1293499995277263E-4 0.0 0.0 7.186772724267349E-5 1.0266818177524786E-5 14-15 1.1293499995277263E-4 0.0 0.0 7.186772724267349E-5 1.0266818177524786E-5 16-17 1.2320181813029742E-4 0.0 0.0 7.186772724267349E-5 1.0266818177524786E-5 18-19 1.386020453965846E-4 0.0 0.0 8.213454542019829E-5 1.0266818177524786E-5 20-21 1.5400227266287178E-4 0.0 0.0 8.213454542019829E-5 1.0266818177524786E-5 22-23 1.5913568175163418E-4 0.0 0.0 8.213454542019829E-5 1.0266818177524786E-5 24-25 1.8480272719544614E-4 0.0 0.0 8.213454542019829E-5 2.0533636355049572E-5 26-27 2.156031817280205E-4 0.0 0.0 8.213454542019829E-5 3.0800454532574356E-5 28-29 2.4127022717183245E-4 0.0 0.0 9.753477268648547E-5 3.0800454532574356E-5 30-31 2.6693727261564444E-4 0.0 0.0 1.1293499995277263E-4 3.0800454532574356E-5 32-33 2.9773772714821877E-4 0.0 0.0 1.1806840904153503E-4 3.0800454532574356E-5 34-35 3.439384089470803E-4 0.0 0.0 1.2320181813029742E-4 3.0800454532574356E-5 36-37 3.6960545439089227E-4 0.0 0.0 1.3346863630782222E-4 3.0800454532574356E-5 38-39 4.05539318012229E-4 0.0 0.0 1.3346863630782222E-4 3.0800454532574356E-5 40-41 4.774070452549025E-4 0.0 0.0 1.4373545448534699E-4 3.0800454532574356E-5 42-43 5.082074997874769E-4 0.0 0.0 1.5400227266287178E-4 3.0800454532574356E-5 44-45 5.492747724975761E-4 0.0 0.0 1.6426909084039657E-4 4.6200681798861534E-5 46-47 5.954754542964375E-4 0.0 0.0 1.7453590901792134E-4 6.160090906514871E-5 48-49 6.468095451840614E-4 0.0 0.0 1.7453590901792134E-4 6.160090906514871E-5 50-51 6.827434088053981E-4 0.0 0.0 1.7453590901792134E-4 6.160090906514871E-5 52-53 7.340774996930221E-4 5.133409088762393E-6 0.0 1.8480272719544614E-4 6.160090906514871E-5 54-55 7.70011363314359E-4 1.0266818177524786E-5 0.0 1.8993613628420853E-4 6.160090906514871E-5 56-57 8.470124996457949E-4 1.0266818177524786E-5 0.0 2.002029544617333E-4 6.160090906514871E-5 58-59 8.829463632671316E-4 1.0266818177524786E-5 0.0 2.053363635504957E-4 6.160090906514871E-5 60-61 9.342804541547555E-4 1.0266818177524786E-5 0.0 2.104697726392581E-4 6.160090906514871E-5 62-63 9.907479541311419E-4 1.0266818177524786E-5 0.0 2.3100340899430766E-4 6.673431815391111E-5 64-65 0.00107288249955134 1.0266818177524786E-5 0.0 2.5667045443811964E-4 7.186772724267349E-5 66-67 0.001206351135859162 1.0266818177524786E-5 0.0 2.6180386352688207E-4 7.186772724267349E-5 68-69 0.001334686363078222 1.0266818177524786E-5 0.0 2.8233749988193155E-4 9.753477268648547E-5 70-71 0.0014219543175871827 1.0266818177524786E-5 0.0 3.1827136350326835E-4 1.0266818177524785E-4 72-73 0.001529755908451193 1.0266818177524786E-5 0.0 3.1827136350326835E-4 1.0266818177524785E-4 74-75 0.0016632245447590153 1.0266818177524786E-5 0.0 3.336715907695555E-4 1.0266818177524785E-4 76-77 0.001858294090131986 1.0266818177524786E-5 0.0 3.5420522712460505E-4 1.0266818177524785E-4 78-79 0.002130364771836393 1.0266818177524786E-5 0.0 3.7473886347965464E-4 1.0266818177524785E-4 80-81 0.002356234771741938 1.0266818177524786E-5 0.0 3.9013909074594186E-4 1.0266818177524785E-4 82-83 0.0027412404533991176 2.0533636355049572E-5 0.0 4.209395452785162E-4 1.0266818177524785E-4 84-85 0.0032905152258966936 2.0533636355049572E-5 0.0 4.31206363456041E-4 1.0266818177524785E-4 86-87 0.004091327043743627 2.0533636355049572E-5 0.0 4.31206363456041E-4 1.0266818177524785E-4 88-89 0.005441413634088136 2.0533636355049572E-5 0.0 4.414731816335658E-4 1.1293499995277263E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 279985 0.0 91.713135 1 AGTGGTA 278720 0.0 91.6927 5 TGGTATC 278895 0.0 91.6536 7 GTGGTAT 279195 0.0 91.62726 6 GTATCAA 279460 0.0 91.55914 9 GGTATCA 279565 0.0 91.518684 8 GCAGTGG 281185 0.0 91.064354 3 AGCAGTG 282540 0.0 90.82858 2 CAGTGGT 282085 0.0 90.49446 4 TCAACGC 278515 0.0 46.391033 12-13 CAACGCA 278985 0.0 46.30436 12-13 ATCAACG 278835 0.0 46.300007 10-11 AACGCAG 280005 0.0 46.19329 14-15 CGCAGAG 280595 0.0 46.118073 16-17 ACGCAGA 280235 0.0 46.11215 14-15 TATCAAC 280070 0.0 46.01102 10-11 AGAGTAC 273090 0.0 45.868862 18-19 CAGAGTA 279705 0.0 45.707867 18-19 GAGTACT 165625 0.0 45.697144 20-21 GCAGAGT 283125 0.0 45.429134 16-17 >>END_MODULE