FastQCFastQC Report
Sat 18 Jun 2016
SRR578586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9740116
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1314711.349788852617361No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133640.13720575812444122No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108300.11118964086259343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4900.025.203963
CGCGTAG2700.021.99097654-55
ACTCGCG2850.021.6680150-51
CAACGCA72600.019.43072912-13
ATCAACG74000.019.0631210-11
CTCGCGT3250.019.00098252-53
CGTAGCG3550.018.0628356-57
TCAACGC69650.017.2190610-11
CGTACGC3600.017.15199320-21
GTATCAA95550.016.9509939
ACGCAGA85050.016.7539114-15
AGTGGTA98500.016.3469665
TCGCGTA4150.016.02492552-53
GGTATCA102200.015.84818
TGGTATC102350.015.8248727
GTGGTAT104950.015.297066
ACGTACG4200.015.267158520-21
AACGCAG78700.015.20880512-13
CGCTAGT3750.015.1994690-91
CGCAGAG81850.014.73955214-15
Produced by FastQC (version 0.11.5)