##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578586_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9740116 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.517705333283505 31.0 31.0 34.0 30.0 34.0 2 31.835478756105164 31.0 31.0 34.0 30.0 34.0 3 31.90799319022484 31.0 31.0 34.0 30.0 34.0 4 35.57233589415157 37.0 35.0 37.0 33.0 37.0 5 35.39487096457578 37.0 35.0 37.0 33.0 37.0 6 35.39270322858578 37.0 35.0 37.0 33.0 37.0 7 35.36944950142278 37.0 35.0 37.0 32.0 37.0 8 35.35547625921498 37.0 35.0 37.0 32.0 37.0 9 36.931693421310385 39.0 37.0 39.0 33.0 39.0 10-11 36.89776035521548 39.0 37.0 39.0 33.0 39.0 12-13 36.88026015295917 39.0 37.0 39.0 33.0 39.0 14-15 38.07252079954694 40.0 37.0 41.0 33.0 41.0 16-17 38.0257453812665 40.0 37.0 41.0 33.0 41.0 18-19 37.967263377561416 40.0 37.0 41.0 33.0 41.0 20-21 37.91442319578124 40.0 37.0 41.0 33.0 41.0 22-23 37.82776170222203 40.0 37.0 41.0 32.0 41.0 24-25 37.74303909727564 40.0 37.0 41.0 32.0 41.0 26-27 37.560925865769974 39.5 36.5 41.0 32.0 41.0 28-29 37.39277791968802 39.0 36.0 41.0 31.5 41.0 30-31 37.2648126059279 39.0 36.0 41.0 31.0 41.0 32-33 37.13907329235093 39.0 36.0 41.0 31.0 41.0 34-35 36.98639461788751 39.0 36.0 40.5 30.5 41.0 36-37 36.83921603192405 39.0 35.5 40.0 30.0 41.0 38-39 36.675692774090166 39.0 35.0 40.0 30.0 41.0 40-41 36.51131942371117 38.0 35.0 40.0 30.0 41.0 42-43 36.45594718789797 38.5 35.0 40.0 30.0 41.0 44-45 36.49000494449963 39.0 35.0 40.0 30.0 41.0 46-47 36.36392697992508 38.0 35.0 40.0 30.0 41.0 48-49 36.22630259228946 38.0 35.0 40.0 29.5 41.0 50-51 36.034956308528564 38.0 35.0 40.0 29.0 41.0 52-53 36.09199105020926 38.0 35.0 40.0 29.0 41.0 54-55 36.08431449892383 38.0 35.0 40.0 29.0 41.0 56-57 35.92942773987497 38.0 34.5 40.0 28.5 41.0 58-59 35.72542031326937 38.0 34.0 40.0 28.0 41.0 60-61 35.47223467359116 38.0 34.0 40.0 28.0 41.0 62-63 35.194265653509675 37.5 34.0 40.0 28.0 41.0 64-65 34.87396941679134 37.0 34.0 40.0 27.0 41.0 66-67 34.53633904360071 36.5 33.0 39.5 26.5 41.0 68-69 34.170499098778706 36.0 33.0 39.0 26.0 41.0 70-71 33.78996584845601 35.5 32.5 39.0 26.0 40.0 72-73 33.401443524902575 35.0 32.0 38.5 26.0 40.0 74-75 32.99432029351601 35.0 32.0 37.5 25.5 40.0 76-77 31.63327587679654 34.0 30.5 36.0 23.5 39.0 78-79 32.277276831199956 35.0 31.0 36.0 25.0 39.0 80-81 32.150005092341814 35.0 31.0 36.0 25.0 39.0 82-83 31.840406777496284 35.0 31.0 36.0 24.5 37.5 84-85 31.51501758295281 34.0 31.0 35.0 24.0 37.0 86-87 31.164721806187934 34.0 31.0 35.0 23.0 36.5 88-89 30.845772576014497 34.0 31.0 35.0 21.0 36.0 90-91 30.524415828312517 34.0 31.0 35.0 20.0 36.0 92-93 30.168070380270628 34.0 30.0 35.0 18.5 36.0 94-95 29.802464929575788 34.0 30.0 35.0 16.5 35.0 96-97 29.420055777569793 34.0 30.0 35.0 4.5 35.0 98-99 28.953060055958268 34.0 29.0 35.0 2.0 35.0 100-101 27.84079907261885 33.0 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37558677523760053 1101 2 0.40346909075378434 1101 3 0.4133418451410158 1101 4 0.31753907302157103 1101 5 0.3602985786317561 1101 6 0.303927143155164 1101 7 0.32531383439757633 1101 8 0.3195880962410058 1101 9 0.42861561520570746 1101 10-11 0.4378002780470638 1101 12-13 0.405096829270299 1101 14-15 0.5260578202838886 1101 16-17 0.5097222753862809 1101 18-19 0.4392968998883404 1101 20-21 0.44098122427926256 1101 22-23 0.5103567126211814 1101 24-25 0.494694368501321 1101 26-27 0.5399118928335795 1101 28-29 0.6042566997595387 1101 30-31 0.6688023505695071 1101 32-33 0.6640290538422704 1101 34-35 0.6811277769674433 1101 36-37 0.5677461213180237 1101 38-39 0.5951827275085719 1101 40-41 0.5816190821486487 1101 42-43 0.6198875792836489 1101 44-45 0.525222234634704 1101 46-47 0.5592761985027508 1101 48-49 0.570011681508241 1101 50-51 0.6244224034216828 1101 52-53 0.6325909756144483 1101 54-55 0.5582992144520205 1101 56-57 0.717009169777377 1101 58-59 0.6257088994925155 1101 60-61 0.6254790402816184 1101 62-63 0.5963428289881065 1101 64-65 0.6272384177420207 1101 66-67 0.5895259380725051 1101 68-69 0.7058209745955466 1101 70-71 0.6785232043713023 1101 72-73 0.6824956817415142 1101 74-75 0.6446534704261708 1101 76-77 0.5763700836035959 1101 78-79 0.7074758688674265 1101 80-81 0.6717283723507208 1101 82-83 0.6663097449989124 1101 84-85 0.7976692458868797 1101 86-87 0.7367410402983836 1101 88-89 0.7027317354436207 1101 90-91 0.7965937542933048 1101 92-93 0.8220740326555216 1101 94-95 0.8817048951251145 1101 96-97 0.8500906807671988 1101 98-99 0.9789814415772682 1101 100-101 0.9927880861739382 1102 1 0.33112811857107616 1102 2 0.2881873481111832 1102 3 0.3395828468845501 1102 4 0.23277945134667988 1102 5 0.28227536196728664 1102 6 0.21672237228877833 1102 7 0.2187201564063912 1102 8 0.21545271288119494 1102 9 0.37505752276418036 1102 10-11 0.2738949002865567 1102 12-13 0.24386550987353672 1102 14-15 0.44144570342761114 1102 16-17 0.43198377921226694 1102 18-19 0.40833942142648993 1102 20-21 0.4263587887395488 1102 22-23 0.4261862825449043 1102 24-25 0.37092946778006564 1102 26-27 0.4175553393627851 1102 28-29 0.37181443899445554 1102 30-31 0.3277992210784504 1102 32-33 0.4171655314544651 1102 34-35 0.4197222983440838 1102 36-37 0.4460148526042289 1102 38-39 0.46205351851318 1102 40-41 0.38965252336871714 1102 42-43 0.4275482255660492 1102 44-45 0.35156386553197905 1102 46-47 0.3220323142725974 1102 48-49 0.3685458059179254 1102 50-51 0.36308481044650165 1102 52-53 0.37374739716345573 1102 54-55 0.3557471198040716 1102 56-57 0.399632014805654 1102 58-59 0.5029086143955155 1102 60-61 0.4876359252317215 1102 62-63 0.3995628645340261 1102 64-65 0.41292235534201893 1102 66-67 0.43646720356549906 1102 68-69 0.31858799458891696 1102 70-71 0.3286793770657681 1102 72-73 0.38447371016118126 1102 74-75 0.2982804287437304 1102 76-77 0.2489415520332976 1102 78-79 0.36114693743881077 1102 80-81 0.42034470120068335 1102 82-83 0.3612922247241812 1102 84-85 0.2132961975181189 1102 86-87 0.2759470063936007 1102 88-89 0.4106956940880018 1102 90-91 0.4416461206948199 1102 92-93 0.3933717176981162 1102 94-95 0.2873428866538035 1102 96-97 0.2797562805759348 1102 98-99 0.4119252219714298 1102 100-101 0.5937554996671253 1103 1 0.24414146093947053 1103 2 0.26485298925348744 1103 3 0.3162728149490128 1103 4 0.16449288944934182 1103 5 0.16414336153783893 1103 6 0.16993153280225215 1103 7 0.21774420838353592 1103 8 0.19266100919293194 1103 9 0.3639065881947303 1103 10-11 0.33510039474218445 1103 12-13 0.31595476772454134 1103 14-15 0.4234884235759395 1103 16-17 0.3963065080598369 1103 18-19 0.3655986413351755 1103 20-21 0.3661469873816472 1103 22-23 0.3597356466171888 1103 24-25 0.3482699244756873 1103 26-27 0.3334803769523873 1103 28-29 0.36860177196297883 1103 30-31 0.37279924875331005 1103 32-33 0.34911610781387736 1103 34-35 0.3411955118736998 1103 36-37 0.4415918823262146 1103 38-39 0.4416586644770959 1103 40-41 0.44820556964768343 1103 42-43 0.3397579917545812 1103 44-45 0.30584536962691544 1103 46-47 0.3812123566522345 1103 48-49 0.38196630654172026 1103 50-51 0.41816979328923054 1103 52-53 0.361999774086172 1103 54-55 0.35861467000548686 1103 56-57 0.41381131950013383 1103 58-59 0.44368897209849933 1103 60-61 0.4900822009292014 1103 62-63 0.4009690736144975 1103 64-65 0.38969664360747913 1103 66-67 0.3349308179839312 1103 68-69 0.3236686883475457 1103 70-71 0.30431987217242806 1103 72-73 0.3422410797753628 1103 74-75 0.3618689198769047 1103 76-77 0.305404815157285 1103 78-79 0.38270912614205344 1103 80-81 0.42693403727720636 1103 82-83 0.3080249926239702 1103 84-85 0.41721801143294 1103 86-87 0.46876478269648914 1103 88-89 0.40382152468344046 1103 90-91 0.38469038510000075 1103 92-93 0.3773628512139027 1103 94-95 0.3445334957279975 1103 96-97 0.42939851727047795 1103 98-99 0.5783887531372649 1103 100-101 0.7504307718658936 1104 1 0.18385021783632283 1104 2 0.19485897043930223 1104 3 0.215420416104525 1104 4 0.18157129566473884 1104 5 0.15753573747384308 1104 6 0.24221105616469174 1104 7 0.17674447454218978 1104 8 0.17791501228673923 1104 9 0.30035108917358144 1104 10-11 0.2950755495846593 1104 12-13 0.27402243884760225 1104 14-15 0.24804164241692206 1104 16-17 0.22868480527415613 1104 18-19 0.2471821474713778 1104 20-21 0.3071571077795099 1104 22-23 0.3292667549215338 1104 24-25 0.28026857441421527 1104 26-27 0.3032916530498042 1104 28-29 0.3211074415721882 1104 30-31 0.25630965249851556 1104 32-33 0.19376850897698006 1104 34-35 0.26589168123651064 1104 36-37 0.30529925472391284 1104 38-39 0.3358066742033188 1104 40-41 0.3349239918185418 1104 42-43 0.24924957448475737 1104 44-45 0.26453081432513414 1104 46-47 0.2693784672963986 1104 48-49 0.19004695307477704 1104 50-51 0.22990004010109288 1104 52-53 0.26132197467311613 1104 54-55 0.2541536786427727 1104 56-57 0.3166078327836388 1104 58-59 0.31835051491814426 1104 60-61 0.26097117443663365 1104 62-63 0.24279734404318987 1104 64-65 0.3417333158371534 1104 66-67 0.3348361746442521 1104 68-69 0.39814464342401124 1104 70-71 0.24797513377250624 1104 72-73 0.29324675642681086 1104 74-75 0.30352972453676585 1104 76-77 0.3045290252129007 1104 78-79 0.3503457000173924 1104 80-81 0.3270819736274575 1104 82-83 0.2646660668448675 1104 84-85 0.2642646148340688 1104 86-87 0.3723385397148036 1104 88-89 0.3069446534281006 1104 90-91 0.5106585454771952 1104 92-93 0.46098805257838293 1104 94-95 0.47384325615567846 1104 96-97 0.5194942751130824 1104 98-99 0.5945037180826631 1104 100-101 0.6791704199429631 1105 1 0.16773421614928452 1105 2 0.14259481258165607 1105 3 0.11247868098232416 1105 4 0.11469121840467977 1105 5 0.13547382883475478 1105 6 0.16124414015435207 1105 7 0.1830487201771831 1105 8 0.13566746276568153 1105 9 0.2189522828602577 1105 10-11 0.1386576531687993 1105 12-13 0.17716797208127133 1105 14-15 0.24084825890421513 1105 16-17 0.15473020953094618 1105 18-19 0.211216226963181 1105 20-21 0.17380468876942956 1105 22-23 0.13540700048513798 1105 24-25 0.14894724230700263 1105 26-27 0.21815024670333827 1105 28-29 0.20016662796962947 1105 30-31 0.16236526960818765 1105 32-33 0.23365634415930714 1105 34-35 0.24171678686751363 1105 36-37 0.24724042667519086 1105 38-39 0.24927095360350648 1105 40-41 0.3545591078430732 1105 42-43 0.20267526072864683 1105 44-45 0.20333168282346747 1105 46-47 0.22206678852866446 1105 48-49 0.2537895226451141 1105 50-51 0.23771362209715363 1105 52-53 0.13097942022795905 1105 54-55 0.2238635031721543 1105 56-57 0.20142294951342166 1105 58-59 0.18382491249605692 1105 60-61 0.10351802393766718 1105 62-63 0.19477257029433304 1105 64-65 0.2485077847952084 1105 66-67 0.17620569218139792 1105 68-69 0.21433742137840994 1105 70-71 0.15942987068171988 1105 72-73 0.21003157582136112 1105 74-75 0.2975321510788689 1105 76-77 0.18036052302079852 1105 78-79 0.20849375499941658 1105 80-81 0.20096236440380721 1105 82-83 0.2946656432717063 1105 84-85 0.3676761634344672 1105 86-87 0.39158752476507175 1105 88-89 0.4182585770634226 1105 90-91 0.30519287649249094 1105 92-93 0.34656611204594867 1105 94-95 0.31904489827275384 1105 96-97 0.4447564347816453 1105 98-99 0.5881472097127443 1105 100-101 0.6826816229719981 1106 1 0.0407132586527581 1106 2 0.0961821782522243 1106 3 -0.019229225850704523 1106 4 0.06594566335907359 1106 5 0.09316629507011953 1106 6 0.18341826975445485 1106 7 0.11803648588121263 1106 8 0.12205861123790385 1106 9 0.0039551280068153005 1106 10-11 0.023208689705036534 1106 12-13 0.010617712006471436 1106 14-15 0.19697034127521107 1106 16-17 0.16185567436070158 1106 18-19 0.0642536046409461 1106 20-21 0.1408286168443098 1106 22-23 0.03664720589267745 1106 24-25 0.1948676535729561 1106 26-27 0.2735349559386506 1106 28-29 0.2571226051699895 1106 30-31 0.2141137831862565 1106 32-33 0.15939387477543931 1106 34-35 0.06070529167972438 1106 36-37 0.07737556257195877 1106 38-39 0.18636254743041292 1106 40-41 0.09994937917689128 1106 42-43 0.08122993953872282 1106 44-45 0.08637085191026728 1106 46-47 0.1249132391351182 1106 48-49 0.2522280350440198 1106 50-51 0.16130162763560207 1106 52-53 -0.026491346706194463 1106 54-55 -0.014614261087480429 1106 56-57 0.058797725214311924 1106 58-59 0.1324433559638365 1106 60-61 0.23914006558477752 1106 62-63 0.16419589240658894 1106 64-65 0.16233957336517335 1106 66-67 0.2072355821893268 1106 68-69 0.269948073598286 1106 70-71 0.13203766825094476 1106 72-73 0.13047743222374208 1106 74-75 0.12579304875271902 1106 76-77 0.0375514057765578 1106 78-79 0.17056605609140263 1106 80-81 0.26565543834789196 1106 82-83 0.3008537955929711 1106 84-85 0.4144660324803695 1106 86-87 0.5489822590392137 1106 88-89 0.4685151154577589 1106 90-91 0.5612500990260543 1106 92-93 0.45789632664039814 1106 94-95 0.620873450592125 1106 96-97 0.5990503516273336 1106 98-99 0.7109265283429664 1106 100-101 0.645685158380175 1107 1 0.0668699447782437 1107 2 0.09104727347663655 1107 3 0.08208909257448127 1107 4 0.11514247948667133 1107 5 0.01797084427841611 1107 6 -0.009348699565016716 1107 7 0.041186710964964846 1107 8 9.644538716599982E-4 1107 9 -0.0696400734727689 1107 10-11 -0.07441987282339113 1107 12-13 -0.08244151921314113 1107 14-15 -0.027227120118091364 1107 16-17 0.11228688380000307 1107 18-19 0.033536189139553585 1107 20-21 -0.05117939248739134 1107 22-23 -0.15732702853143365 1107 24-25 -0.05112688365387186 1107 26-27 -0.01279661419646061 1107 28-29 4.5966674816355635E-4 1107 30-31 0.04679454326753785 1107 32-33 0.012485278093514296 1107 34-35 0.01515167185691979 1107 36-37 0.05128070314116684 1107 38-39 0.007050817206810223 1107 40-41 -0.03867993676688286 1107 42-43 -0.016445235709838357 1107 44-45 -0.03440705280841172 1107 46-47 0.06076180083591254 1107 48-49 -0.04724193712957003 1107 50-51 0.05125683421703542 1107 52-53 -2.459800134744228E-4 1107 54-55 0.02354117480670226 1107 56-57 -0.001943585210618437 1107 58-59 0.04354594853715099 1107 60-61 0.16954184569127762 1107 62-63 0.14387588098963278 1107 64-65 0.15001207617044088 1107 66-67 0.06485269376284464 1107 68-69 0.2346225770363688 1107 70-71 0.16246101452861694 1107 72-73 0.07101258875341898 1107 74-75 0.060373755091482906 1107 76-77 0.05817160098605001 1107 78-79 0.07595442954693254 1107 80-81 0.184497423245503 1107 82-83 0.17551547060772066 1107 84-85 0.22160914132653176 1107 86-87 0.20217997471358018 1107 88-89 0.14006768001997827 1107 90-91 0.15442760320086535 1107 92-93 0.22125080092638427 1107 94-95 0.13648506531315974 1107 96-97 0.2579456621336398 1107 98-99 0.2554616232826099 1107 100-101 0.5190517039648554 1108 1 0.023477937293659323 1108 2 0.05541647869304356 1108 3 -0.013360035385378666 1108 4 0.04636930457102295 1108 5 -0.033356159379280825 1108 6 -0.011990026611691462 1108 7 0.04665274496076677 1108 8 0.04739111223959469 1108 9 -0.037353101523528665 1108 10-11 -0.08234085449945638 1108 12-13 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5 667.0 6 1220.0 7 2262.0 8 4121.0 9 6380.0 10 8862.0 11 11459.0 12 14067.0 13 16890.0 14 19275.0 15 22063.0 16 25220.0 17 29578.0 18 34638.0 19 39497.0 20 42537.0 21 44513.0 22 50477.0 23 59483.0 24 73015.0 25 92711.0 26 118608.0 27 150430.0 28 188993.0 29 234708.0 30 289409.0 31 351352.0 32 427629.0 33 530115.0 34 683378.0 35 953639.0 36 1237740.0 37 1786777.0 38 1766010.0 39 420985.0 40 875.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.58109467305343 31.30184933804149 29.648407994872056 22.468647994033027 2 21.711014530011756 30.20459920600535 28.31771202724896 19.766674236733937 3 20.786662089034667 29.20867677551274 28.757850522519448 21.24681061293315 4 21.051163373300014 29.141068894779963 28.857725739185074 20.95004199273495 5 21.618618982737413 29.172211196190258 29.009430209050624 20.19973961202171 6 21.595260868261764 29.281093288180752 28.04397982261213 21.079666020945353 7 21.869700717085987 29.301604755179994 28.485392626267757 20.34330190146626 8 21.08275446368483 29.397974538872944 28.479206020716617 21.040064976725606 9 20.745144606377597 29.126283765580958 29.065432209292393 21.063139418749056 10-11 20.838872075524872 29.555869663589267 28.40364359615312 21.201614664732737 12-13 20.81526758154293 29.62057429506736 28.32497870918362 21.239179414206095 14-15 20.99776244918694 29.66274316707176 28.129254955332144 21.210239428409153 16-17 21.14990053502465 29.45372188738692 28.125087267990345 21.271290309598083 18-19 21.12624223366539 29.469874896767145 28.14018334073229 21.263699528835183 20-21 21.22368368232505 29.389379397988137 28.129162236102744 21.25777468358407 22-23 21.1534216596178 29.34940695668565 28.19165166510645 21.305519718590098 24-25 21.19166862035671 29.322095821142984 28.10345524136752 21.38278031713279 26-27 21.38904417452977 29.22779659172402 28.20141240632169 21.181746827424522 28-29 21.348099913183567 29.277503588345848 28.12927912071191 21.245117377758678 30-31 21.31883860666311 29.288828409491792 28.091656676888476 21.300676306956625 32-33 21.3769874512857 29.31718795166139 27.994847887053105 21.31097670999981 34-35 21.369770991802127 29.33278426231878 28.01886389527487 21.278580850604225 36-37 21.41117461557957 29.326147016486352 27.9742490940402 21.28842927389388 38-39 21.42655873980999 29.307617978927393 27.96361269925133 21.302210582011288 40-41 21.401056842313203 29.342939242193527 27.941745846333728 21.314258069159546 42-43 21.43688870041181 29.3186688765781 27.923246046212146 21.321196376797943 44-45 21.40820158065737 29.332063252372688 27.926317508364807 21.333417658605132 46-47 21.42754651166432 29.384631014915552 27.875097457863575 21.312725015556552 48-49 21.434676942411475 29.368285034868535 27.847186285864485 21.349851736855506 50-51 21.46699878037079 29.375013572398522 27.83159249835373 21.326395148876955 52-53 21.431935228749595 29.39982478022445 27.821527389705363 21.34671260132059 54-55 21.442765629515215 29.422017227225457 27.748172250487862 21.387044892771463 56-57 21.452871888428838 29.44773088163072 27.72073904051754 21.378658189422907 58-59 21.454053319004952 29.52701101286274 27.647530282125647 21.371405386006657 60-61 21.413557516447103 29.516359011393618 27.70834826315445 21.361735209004834 62-63 21.430059764211116 29.504760587663696 27.682681356154422 21.382498291970766 64-65 21.42056950233035 29.55438030678541 27.690434935986826 21.33461525489741 66-67 21.413062187440502 29.576288940334006 27.642807212758914 21.36784165946658 68-69 21.39520942191616 29.587994966537266 27.662253480218123 21.35454213132845 70-71 21.425877819536908 29.612958375915532 27.61046063105224 21.350703173495322 72-73 21.40248490709448 29.615606661340777 27.61542000531631 21.366488426248438 74-75 21.43480380090744 29.647067761767982 27.580582938934818 21.337545498389765 76-77 21.401931968777372 29.708737555076347 27.59342907209729 21.295901404048987 78-79 21.41467822354477 29.715636856891642 27.5494357562066 21.320249163356987 80-81 21.40537389456408 29.74706485468766 27.523675179904366 21.323886070843894 82-83 21.36046708369199 29.798448598453238 27.530709142907835 21.31037517494694 84-85 21.380154137013268 29.822377089435577 27.53248798613526 21.2649807874159 86-87 21.368966355959447 29.86166175709764 27.509560492013048 21.259811394929866 88-89 21.355103396059356 29.925804037710456 27.47768584655538 21.241406719674806 90-91 21.347397105623877 29.938914140270782 27.434242968293525 21.279445785811813 92-93 21.366190928251253 29.970206009691015 27.42582521252583 21.237777849531906 94-95 21.342109444201036 30.02727134358017 27.408241874872147 21.22237733734665 96-97 21.307922125103303 30.045770503335905 27.40254331452448 21.24376405703631 98-99 21.320229724175242 30.097656439494997 27.33944992746749 21.242663908862273 100-101 21.352008816323917 30.08002267735265 27.273351215396453 21.294617290926983 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 141383.0 1 80459.5 2 14283.0 3 7911.0 4 6184.5 5 5428.0 6 5402.5 7 5314.0 8 4703.5 9 4390.5 10 4471.0 11 5286.0 12 9249.0 13 8996.5 14 5749.5 15 6024.0 16 6615.0 17 7823.0 18 9689.0 19 12262.5 20 16146.5 21 21326.0 22 28027.0 23 37918.0 24 48941.5 25 61837.5 26 83259.0 27 106080.5 28 119316.0 29 129460.0 30 146847.5 31 170033.0 32 195104.5 33 221946.5 34 247302.0 35 266562.0 36 282719.0 37 298461.0 38 308457.0 39 311763.0 40 318221.0 41 327878.5 42 333460.0 43 336226.0 44 340693.5 45 346391.0 46 346014.0 47 342644.5 48 338802.5 49 332796.5 50 326710.0 51 316181.0 52 305328.5 53 295354.5 54 280095.5 55 259255.5 56 235394.0 57 211298.0 58 186716.5 59 156553.0 60 121894.5 61 91856.5 62 67295.5 63 47751.5 64 33573.5 65 22603.5 66 14142.0 67 8993.5 68 5723.5 69 3475.0 70 1973.5 71 1020.5 72 527.5 73 272.5 74 173.0 75 106.0 76 67.5 77 46.0 78 38.5 79 31.0 80 26.0 81 18.0 82 13.0 83 11.5 84 7.0 85 5.0 86 3.5 87 2.0 88 2.5 89 2.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1497107426646664 2 0.0 3 0.0 4 0.0034496509076483274 5 3.593386362133675E-4 6 0.0010677490904625777 7 1.0266818177524786E-5 8 1.3346863630782222E-4 9 2.053363635504957E-4 10-11 1.386020453965846E-4 12-13 1.0266818177524786E-5 14-15 2.0533636355049572E-5 16-17 4.1067272710099144E-5 18-19 0.0 20-21 9.240136359772307E-5 22-23 1.8480272719544614E-4 24-25 5.133409088762393E-6 26-27 1.0266818177524786E-5 28-29 0.006165224315603633 30-31 0.0016426909084039656 32-33 3.542052271246051E-4 34-35 2.8747090897069397E-4 36-37 3.234047725920307E-4 38-39 0.001278218863101836 40-41 0.0035625859076011002 42-43 5.903420452076752E-4 44-45 5.595415906751008E-4 46-47 0.006678565224479873 48-49 0.006940369088006755 50-51 0.006986569769805616 52-53 0.009430072496056515 54-55 0.00487160522523551 56-57 5.133409088762393E-6 58-59 5.646749997638632E-5 60-61 0.001047215454107528 62-63 9.188802268884683E-4 64-65 5.184743179650017E-4 66-67 7.238106815154973E-4 68-69 0.0010420820450187658 70-71 0.001334686363078222 72-73 0.002823374998819316 74-75 7.546111360480717E-4 76-77 0.0 78-79 0.0 80-81 1.0780159086401023E-4 82-83 2.053363635504957E-4 84-85 1.5913568175163415E-4 86-87 0.003008177726014762 88-89 0.001699158408380352 90-91 5.646749997638632E-4 92-93 6.622097724503486E-4 94-95 3.952724998347043E-4 96-97 0.001124216590438964 98-99 5.133409088762393E-6 100-101 2.0533636355049572E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9740116.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.85494674115043 #Duplication Level Percentage of deduplicated Percentage of total 1 89.06986700628853 53.31272145902752 2 6.045169852063602 7.236666390729503 3 1.5109214801553499 2.713083741742759 4 0.7617777581242319 1.823846685644755 5 0.5162779282056589 1.5450893948190598 6 0.36750007622992803 1.319801849406664 7 0.26571858085279393 1.1133200053554637 8 0.1782620045286497 0.8535890229626435 9 0.13892792717346683 0.7483971313643654 >10 0.9612926537793125 12.55753018459961 >50 0.14038137073111415 5.741002113251061 >100 0.04331183652860961 4.237211592291083 >500 1.3145007526504558E-4 0.05542961795520155 >1k 1.3145007526504544E-4 0.17942830907996737 >5k 1.3145007526504544E-4 0.43907023846558124 >10k+ 1.9717511289756817E-4 6.123812263304785 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 131471 1.349788852617361 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13364 0.13720575812444122 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10830 0.11118964086259343 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.0533636355049572E-5 0.0 0.0 2.0533636355049572E-5 1.0266818177524786E-5 2 2.0533636355049572E-5 0.0 0.0 2.0533636355049572E-5 1.0266818177524786E-5 3 4.1067272710099144E-5 0.0 0.0 2.0533636355049572E-5 2.0533636355049572E-5 4 5.1334090887623924E-5 0.0 0.0 2.0533636355049572E-5 2.0533636355049572E-5 5 6.160090906514871E-5 0.0 0.0 3.0800454532574356E-5 2.0533636355049572E-5 6 7.186772724267349E-5 0.0 0.0 3.0800454532574356E-5 4.1067272710099144E-5 7 7.186772724267349E-5 0.0 0.0 3.0800454532574356E-5 4.1067272710099144E-5 8 7.186772724267349E-5 0.0 0.0 4.1067272710099144E-5 4.1067272710099144E-5 9 7.186772724267349E-5 0.0 0.0 4.1067272710099144E-5 4.1067272710099144E-5 10-11 8.213454542019827E-5 0.0 0.0 4.1067272710099144E-5 4.6200681798861534E-5 12-13 1.0266818177524785E-4 0.0 0.0 4.1067272710099144E-5 6.673431815391111E-5 14-15 1.2320181813029742E-4 0.0 0.0 5.1334090887623924E-5 7.186772724267349E-5 16-17 1.4373545448534699E-4 0.0 0.0 6.160090906514871E-5 8.213454542019827E-5 18-19 1.4373545448534699E-4 0.0 0.0 8.213454542019829E-5 9.240136359772307E-5 20-21 1.5913568175163418E-4 5.133409088762393E-6 0.0 9.240136359772307E-5 1.2320181813029742E-4 22-23 1.7966931810668374E-4 1.0266818177524786E-5 0.0 1.1293499995277263E-4 1.386020453965846E-4 24-25 2.104697726392581E-4 1.0266818177524786E-5 0.0 1.2833522721905982E-4 1.5400227266287178E-4 26-27 2.4127022717183245E-4 1.0266818177524786E-5 0.0 1.4886886357410938E-4 1.5400227266287178E-4 28-29 2.6180386352688207E-4 1.0266818177524786E-5 0.0 1.6426909084039657E-4 1.6426909084039657E-4 30-31 2.926043180594564E-4 1.0266818177524786E-5 0.0 1.6426909084039657E-4 1.8480272719544614E-4 32-33 3.1827136350326835E-4 1.0266818177524786E-5 0.0 1.7966931810668374E-4 2.156031817280205E-4 34-35 3.2853818168079315E-4 1.0266818177524786E-5 0.0 2.002029544617333E-4 2.207365908167829E-4 36-37 3.5420522712460505E-4 1.0266818177524786E-5 0.0 2.104697726392581E-4 2.2586999990554526E-4 38-39 3.7473886347965464E-4 1.0266818177524786E-5 0.0 2.3613681808307005E-4 2.3613681808307005E-4 40-41 3.9013909074594186E-4 1.0266818177524786E-5 0.0 2.5667045443811964E-4 2.4127022717183245E-4 42-43 4.0553931801222897E-4 1.0266818177524786E-5 0.0 2.6180386352688207E-4 2.6693727261564444E-4 44-45 4.2607295436727856E-4 1.0266818177524786E-5 0.0 2.720706817044068E-4 2.8233749988193155E-4 46-47 4.722736361661401E-4 1.0266818177524786E-5 0.0 2.772040907931692E-4 2.8747090897069397E-4 48-49 5.23607727053764E-4 1.0266818177524786E-5 0.0 2.772040907931692E-4 2.9773772714821877E-4 50-51 5.852086361189128E-4 1.0266818177524786E-5 0.0 2.772040907931692E-4 3.1827136350326835E-4 52-53 6.211424997402495E-4 1.0266818177524786E-5 0.0 2.8233749988193155E-4 3.2853818168079315E-4 54-55 6.570763633615862E-4 1.0266818177524786E-5 0.0 2.8747090897069397E-4 3.2853818168079315E-4 56-57 7.135438633379726E-4 1.0266818177524786E-5 0.0 2.8747090897069397E-4 3.593386362133675E-4 58-59 7.751447724031212E-4 1.0266818177524786E-5 0.0 2.9773772714821877E-4 3.6960545439089227E-4 60-61 8.316122723795076E-4 1.0266818177524786E-5 0.0 3.0800454532574356E-4 3.850056816571795E-4 62-63 8.932131814446563E-4 1.0266818177524786E-5 0.0 3.1827136350326835E-4 3.9527249983470423E-4 64-65 9.650809086873298E-4 1.0266818177524786E-5 0.0 3.3880499985831794E-4 4.004059089234666E-4 66-67 0.0010728824995513402 1.0266818177524786E-5 0.0 3.593386362133675E-4 4.106727271009914E-4 68-69 0.0011960843176816375 1.0266818177524786E-5 0.0 3.7473886347965464E-4 4.414731816335658E-4 70-71 0.0013295529539894596 1.0266818177524786E-5 0.0 3.9527249983470423E-4 4.620068179886153E-4 72-73 0.0014476213630309947 1.0266818177524786E-5 0.0 4.363397725448034E-4 4.8254045434366485E-4 74-75 0.0015964902266051041 1.0266818177524786E-5 0.0 4.568734088998529E-4 5.082074997874769E-4 76-77 0.0017812929538005501 1.0266818177524786E-5 0.0 4.722736361661401E-4 5.338745452312889E-4 78-79 0.0020328299991499077 1.0266818177524786E-5 0.0 4.825404543436649E-4 5.544081815863384E-4 80-81 0.002258699999055453 1.0266818177524786E-5 0.0 4.825404543436649E-4 5.698084088526256E-4 82-83 0.0026745061352452066 1.0266818177524786E-5 0.0 4.825404543436649E-4 5.852086361189128E-4 84-85 0.0032340477259203072 1.0266818177524786E-5 0.0 4.928072725211897E-4 5.954754542964375E-4 86-87 0.004014325907412191 1.0266818177524786E-5 0.0 4.979406816099521E-4 6.211424997402495E-4 88-89 0.0053387454523128874 1.0266818177524786E-5 0.0 5.133409088762392E-4 6.365427270065367E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 490 0.0 25.20396 3 CGCGTAG 270 0.0 21.990976 54-55 ACTCGCG 285 0.0 21.66801 50-51 CAACGCA 7260 0.0 19.430729 12-13 ATCAACG 7400 0.0 19.06312 10-11 CTCGCGT 325 0.0 19.000982 52-53 CGTAGCG 355 0.0 18.06283 56-57 TCAACGC 6965 0.0 17.21906 10-11 CGTACGC 360 0.0 17.151993 20-21 GTATCAA 9555 0.0 16.950993 9 ACGCAGA 8505 0.0 16.75391 14-15 AGTGGTA 9850 0.0 16.346966 5 TCGCGTA 415 0.0 16.024925 52-53 GGTATCA 10220 0.0 15.8481 8 TGGTATC 10235 0.0 15.824872 7 GTGGTAT 10495 0.0 15.29706 6 ACGTACG 420 0.0 15.2671585 20-21 AACGCAG 7870 0.0 15.208805 12-13 CGCTAGT 375 0.0 15.19946 90-91 CGCAGAG 8185 0.0 14.739552 14-15 >>END_MODULE