FastQCFastQC Report
Sat 18 Jun 2016
SRR578585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9848382
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT129365913.135751639203272No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT330070.3351515000128955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA223570.2270119091643683No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG129210.13119921627735398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCCGCAGCGGCTTCTGA127640.12960504578315504No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT112290.11401872916789782No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA100670.10221983672038717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC98820.10034135556480242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2816300.091.7345355
AAGCAGT2833750.091.6817251
TGGTATC2820000.091.573247
GTATCAA2831750.091.5653469
GTGGTAT2826550.091.527076
GGTATCA2830650.091.493228
GCAGTGG2850700.090.863183
AGCAGTG2864750.090.639392
CAGTGGT2858050.090.395684
TCAACGC2817850.046.39642712-13
ATCAACG2817200.046.3485410-11
CAACGCA2819350.046.33635312-13
AACGCAG2834600.046.1772214-15
ACGCAGA2833500.046.16580214-15
CGCAGAG2834800.046.1422416-17
TATCAAC2836150.046.02964410-11
AGAGTAC2760800.045.9100318-19
CAGAGTA2825050.045.7478318-19
GAGTACT1676200.045.63028320-21
GCAGAGT2864050.045.45620716-17
Produced by FastQC (version 0.11.5)