##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578585_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9848382 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.64722672211537 31.0 31.0 34.0 27.0 34.0 2 30.946086473899978 31.0 31.0 34.0 27.0 34.0 3 31.042852724437374 31.0 31.0 34.0 27.0 34.0 4 34.48535617322724 37.0 35.0 37.0 30.0 37.0 5 34.536124208017114 37.0 35.0 37.0 30.0 37.0 6 34.52776750536281 37.0 35.0 37.0 30.0 37.0 7 34.43224440319232 37.0 35.0 37.0 30.0 37.0 8 34.39101194490628 37.0 35.0 37.0 30.0 37.0 9 35.827974381984774 39.0 35.0 39.0 30.0 39.0 10-11 35.80206002366683 39.0 35.0 39.0 30.0 39.0 12-13 35.87203898061631 39.0 35.0 39.0 30.0 39.0 14-15 36.91415209117599 40.0 36.0 41.0 31.0 41.0 16-17 36.8733846331306 40.0 36.0 41.0 30.5 41.0 18-19 36.863072076205 40.0 36.0 41.0 30.0 41.0 20-21 36.74481879358457 39.5 36.0 41.0 30.0 41.0 22-23 36.43197050033193 39.0 36.0 40.5 29.5 41.0 24-25 36.323204918330745 39.0 36.0 40.0 29.0 41.0 26-27 36.28832360483173 39.0 36.0 40.5 29.0 41.0 28-29 35.98759938434557 39.0 35.0 41.0 27.0 41.0 30-31 35.70647523623677 39.0 34.5 40.5 26.5 41.0 32-33 35.401721521362596 38.0 34.0 40.0 25.5 41.0 34-35 35.21024844487145 38.0 34.0 40.0 25.0 41.0 36-37 35.19714553111364 38.0 34.0 40.0 25.0 41.0 38-39 34.45454943766397 37.0 33.5 40.0 25.0 41.0 40-41 34.39647756352262 37.0 33.0 40.0 25.0 41.0 42-43 34.1968035460038 37.0 33.0 40.0 24.0 41.0 44-45 34.02088053651859 37.0 33.0 40.0 23.0 41.0 46-47 33.79313058733912 37.0 33.0 40.0 23.0 41.0 48-49 33.39764135875314 37.0 33.0 40.0 20.5 41.0 50-51 32.066806963824106 36.0 31.5 39.0 14.0 40.5 52-53 31.61127320203461 35.5 31.0 39.0 5.0 40.0 54-55 31.218574837978462 37.0 30.0 40.0 2.0 41.0 56-57 30.67504286490918 36.5 28.5 40.0 2.0 41.0 58-59 30.34507394209526 36.0 28.0 40.0 2.0 41.0 60-61 30.04318211864649 36.0 28.0 39.5 2.0 41.0 62-63 29.706300994417155 35.0 27.5 39.0 2.0 41.0 64-65 29.3747898385745 35.0 27.0 39.0 2.0 41.0 66-67 29.074649825727718 35.0 26.0 39.0 2.0 40.5 68-69 28.74215079187627 35.0 26.0 38.0 2.0 40.0 70-71 28.35016223984813 35.0 26.0 37.5 2.0 40.0 72-73 27.96259329705123 34.0 25.5 37.0 2.0 39.5 74-75 27.567119807091153 34.0 24.5 36.5 2.0 39.0 76-77 27.165635482051773 34.0 23.5 36.0 2.0 39.0 78-79 26.787477729844355 34.0 22.0 35.5 2.0 38.5 80-81 26.394883849956265 34.0 21.0 35.0 2.0 37.0 82-83 26.01655784676102 33.0 20.0 35.0 2.0 37.0 84-85 25.604438932202264 33.0 18.5 35.0 2.0 36.0 86-87 25.22289133382519 33.0 16.0 35.0 2.0 36.0 88-89 24.847169971676564 32.5 9.0 35.0 2.0 35.5 90-91 24.463724751944024 32.0 4.5 35.0 2.0 35.0 92-93 24.082811775578975 32.0 2.0 35.0 2.0 35.0 94-95 23.635921920981538 32.0 2.0 35.0 2.0 35.0 96-97 23.16341770658368 31.0 2.0 35.0 2.0 35.0 98-99 22.58716878569495 31.0 2.0 34.0 2.0 35.0 100-101 21.041141428104638 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5106676846944573 1101 2 0.5121757931262003 1101 3 0.5011543275681944 1101 4 0.5976720407801608 1101 5 0.5996429889132031 1101 6 0.5081284173557918 1101 7 0.48838535918573456 1101 8 0.5899359836035245 1101 9 0.5821396869880999 1101 10-11 0.6198673527391989 1101 12-13 0.6261817302357287 1101 14-15 0.788037727680468 1101 16-17 0.7389178661854459 1101 18-19 0.7085219727296419 1101 20-21 0.7208929573534988 1101 22-23 0.7887057234801986 1101 24-25 0.9227165368819499 1101 26-27 0.8366003003906002 1101 28-29 0.7785618140599979 1101 30-31 0.9633039514647237 1101 32-33 0.9803463315910719 1101 34-35 1.0487886653192362 1101 36-37 0.9205044551424209 1101 38-39 1.0339609339657798 1101 40-41 1.0183294940267658 1101 42-43 1.1848848151151614 1101 44-45 1.0836541369125499 1101 46-47 1.0596383285921362 1101 48-49 1.1271919072560053 1101 50-51 1.1357873556229094 1101 52-53 1.1880224777424218 1101 54-55 1.139043083232682 1101 56-57 1.0890887013761095 1101 58-59 1.135098596706861 1101 60-61 1.1942464892210083 1101 62-63 1.126495713197432 1101 64-65 1.179799157247409 1101 66-67 1.1211925909708889 1101 68-69 1.2083396478444506 1101 70-71 1.0851977611572146 1101 72-73 1.1934016811342545 1101 74-75 1.1870773739430796 1101 76-77 1.1452500306326634 1101 78-79 1.198050654499177 1101 80-81 1.2366745852908885 1101 82-83 1.2128523023382058 1101 84-85 1.3722735224462106 1101 86-87 1.4320594069697314 1101 88-89 1.483983928501619 1101 90-91 1.6384928059792117 1101 92-93 1.5884422638610936 1101 94-95 1.6846439817096943 1101 96-97 1.9465435345337383 1101 98-99 2.124006531410714 1101 100-101 2.512233466342277 1102 1 0.5105930464739927 1102 2 0.4393605048177278 1102 3 0.5266294355058321 1102 4 0.5875209550150302 1102 5 0.5637580589722049 1102 6 0.47442927109287325 1102 7 0.5395006589355802 1102 8 0.6168439562604888 1102 9 0.5229162632410436 1102 10-11 0.6834527031579327 1102 12-13 0.6680003884259378 1102 14-15 0.831412575294955 1102 16-17 0.7885703102593027 1102 18-19 0.756058779896918 1102 20-21 0.7546587456188192 1102 22-23 0.801963627741749 1102 24-25 0.8914381479953164 1102 26-27 0.9456649221248767 1102 28-29 0.9161668188949292 1102 30-31 0.9593863596246592 1102 32-33 0.9440466980480835 1102 34-35 1.0488000326934852 1102 36-37 0.9048093899518648 1102 38-39 1.0065382638694729 1102 40-41 1.0624656268230197 1102 42-43 1.177161136537471 1102 44-45 1.1229255988132039 1102 46-47 1.122432022589578 1102 48-49 1.2336497824598354 1102 50-51 1.1041380984427889 1102 52-53 1.1329259864130137 1102 54-55 1.0801760873082067 1102 56-57 0.986806218960286 1102 58-59 1.1612276678353375 1102 60-61 1.1695749189746856 1102 62-63 1.1017211171287506 1102 64-65 1.1674421328124076 1102 66-67 1.1376933830487062 1102 68-69 1.2242275986703 1102 70-71 1.1020128313272153 1102 72-73 1.1623912683316568 1102 74-75 1.3368233306009465 1102 76-77 1.1954442647304369 1102 78-79 1.1862211132300757 1102 80-81 1.2148689603194356 1102 82-83 1.379842123789853 1102 84-85 1.4590612356836594 1102 86-87 1.3978087580529959 1102 88-89 1.615130240010675 1102 90-91 1.540928092216479 1102 92-93 1.485631465768229 1102 94-95 1.5351800304901637 1102 96-97 1.731798798275527 1102 98-99 1.8405036316613739 1102 100-101 2.2650149271538815 1103 1 0.3599494852668599 1103 2 0.298068754606021 1103 3 0.21404207018907684 1103 4 0.38557450958351325 1103 5 0.2920915822868011 1103 6 0.23303879426480734 1103 7 0.4833303205660755 1103 8 0.2990301696976587 1103 9 0.554644076116837 1103 10-11 0.48513663320441935 1103 12-13 0.35696670901254635 1103 14-15 0.5818649047158075 1103 16-17 0.5606335437978913 1103 18-19 0.5824587719022674 1103 20-21 0.7007449256718274 1103 22-23 0.6761833572057085 1103 24-25 0.5773207598020065 1103 26-27 0.7687413252245534 1103 28-29 0.6717207851797724 1103 30-31 0.5677733406619865 1103 32-33 0.8019007024131213 1103 34-35 0.7679703992555105 1103 36-37 0.7893305693154957 1103 38-39 0.5150941745442026 1103 40-41 0.6188427272365615 1103 42-43 0.47861763747575026 1103 44-45 0.556489213681985 1103 46-47 0.760891176467986 1103 48-49 0.7453604113064927 1103 50-51 0.6422584119955701 1103 52-53 0.49552475263701723 1103 54-55 0.5561648382505595 1103 56-57 0.38576271807051654 1103 58-59 0.4933538787424929 1103 60-61 0.4860335554757391 1103 62-63 0.47996972572246577 1103 64-65 0.5528731707901642 1103 66-67 0.4754480124507694 1103 68-69 0.6606438675882309 1103 70-71 0.7143038258657484 1103 72-73 0.6738340276526316 1103 74-75 0.8422290119697777 1103 76-77 0.873193873914726 1103 78-79 0.8845087570840136 1103 80-81 0.8044762828501888 1103 82-83 0.9603718116060769 1103 84-85 0.9831170382600014 1103 86-87 1.1470400854468359 1103 88-89 1.1826999724117009 1103 90-91 1.0108606316691429 1103 92-93 1.128866732477146 1103 94-95 1.174099577498989 1103 96-97 1.350884493855986 1103 98-99 1.4741198678207397 1103 100-101 1.7833559970444313 1104 1 0.12340363807660992 1104 2 0.22790359853233966 1104 3 0.14436116595895854 1104 4 0.14574417420884345 1104 5 0.0012564658847367127 1104 6 0.009781026589038788 1104 7 0.2354826059395947 1104 8 0.2086377848368599 1104 9 0.31031090689150886 1104 10-11 0.34831125106371275 1104 12-13 0.17149792647897755 1104 14-15 0.36742723356277907 1104 16-17 0.518733720301725 1104 18-19 0.5593078696419198 1104 20-21 0.4165349941484777 1104 22-23 0.30813758246726053 1104 24-25 0.3634099964401969 1104 26-27 0.47224096082880607 1104 28-29 0.6196490910572621 1104 30-31 0.534269369029218 1104 32-33 0.7343644664230169 1104 34-35 0.6443722980545488 1104 36-37 0.7442942773345038 1104 38-39 0.4879360961922714 1104 40-41 0.5074529818213449 1104 42-43 0.6304395331456334 1104 44-45 0.8681528276917163 1104 46-47 0.6743498164618345 1104 48-49 0.6561996773646257 1104 50-51 0.7164352748714151 1104 52-53 0.47682333461561655 1104 54-55 0.5776507736191228 1104 56-57 0.6929594174656621 1104 58-59 0.7408540245561959 1104 60-61 0.6465335821060272 1104 62-63 0.5428250650006667 1104 64-65 0.7288473546825642 1104 66-67 0.5052172936315742 1104 68-69 0.5604233426699956 1104 70-71 0.7239936704342824 1104 72-73 0.5414630363719368 1104 74-75 0.6481754627693874 1104 76-77 0.7434399813220871 1104 78-79 0.8298572783352647 1104 80-81 0.7739708403390466 1104 82-83 0.7975934113117411 1104 84-85 0.7176935200971535 1104 86-87 0.8148711777073778 1104 88-89 0.9710945295602649 1104 90-91 1.0551017740508186 1104 92-93 0.9864979566941408 1104 94-95 1.0823390466437424 1104 96-97 1.3230708770803439 1104 98-99 1.5915799443466199 1104 100-101 1.8560018358696873 1105 1 0.0014143412018192691 1105 2 0.1655657074451078 1105 3 0.17491150293388458 1105 4 0.2071092814563542 1105 5 0.19739075086064872 1105 6 0.20064483008207645 1105 7 0.31494199130411005 1105 8 0.36381549423336423 1105 9 0.2614473955256358 1105 10-11 0.2925069890068741 1105 12-13 0.21344121816051853 1105 14-15 0.10142044827129837 1105 16-17 0.3240318558322315 1105 18-19 0.15399062904031524 1105 20-21 0.20377818330107544 1105 22-23 0.36608817615229583 1105 24-25 0.5377656759973561 1105 26-27 0.5255773304693747 1105 28-29 0.5941543971397323 1105 30-31 0.5686386865599786 1105 32-33 0.6513734113431582 1105 34-35 0.5293356789112877 1105 36-37 0.7752372727741488 1105 38-39 0.6930126324287542 1105 40-41 0.6220465891513527 1105 42-43 0.8406815635170304 1105 44-45 0.9272324086797994 1105 46-47 0.7347904453320169 1105 48-49 0.8167749746584292 1105 50-51 0.7390401454614164 1105 52-53 0.6266595407002313 1105 54-55 0.8098237825670402 1105 56-57 0.8237836127549691 1105 58-59 0.9152066180627223 1105 60-61 0.7844958599295815 1105 62-63 0.8810780497425732 1105 64-65 1.0327421857174528 1105 66-67 0.9785908155294614 1105 68-69 0.9538097977273203 1105 70-71 0.8929361848861497 1105 72-73 0.8007581023865882 1105 74-75 0.7356619502762278 1105 76-77 0.7042372685252438 1105 78-79 0.8594651468219894 1105 80-81 0.8631019226296175 1105 82-83 1.011217990208042 1105 84-85 1.0530150553814615 1105 86-87 1.0704696622868006 1105 88-89 0.9653723374885281 1105 90-91 1.1002048023491753 1105 92-93 1.1734556069966118 1105 94-95 1.2836053816881154 1105 96-97 1.4925735026125864 1105 98-99 1.6948777909734858 1105 100-101 2.00765119862535 1106 1 0.23579370266180177 1106 2 0.3140494835470342 1106 3 0.20510696260192063 1106 4 0.09271407679366206 1106 5 0.2880055097003975 1106 6 0.25520610657414977 1106 7 0.3383792229523408 1106 8 0.33463264537996906 1106 9 0.37583206128257274 1106 10-11 0.2140208874911096 1106 12-13 0.3656490122896656 1106 14-15 0.32966053059928413 1106 16-17 0.44345998776843487 1106 18-19 0.3585408458694914 1106 20-21 0.2962607513606983 1106 22-23 0.4750517316070457 1106 24-25 0.6456109221721107 1106 26-27 0.4938564808161203 1106 28-29 0.5386837139954821 1106 30-31 0.6338957433236843 1106 32-33 0.5217604885007461 1106 34-35 0.4235896599520004 1106 36-37 0.4763820378492767 1106 38-39 0.4808595042796995 1106 40-41 0.41282350528816636 1106 42-43 0.5572236461007734 1106 44-45 0.5090473906587505 1106 46-47 0.6434798949867471 1106 48-49 0.7350957417826152 1106 50-51 0.5545164628741404 1106 52-53 0.5167310708335862 1106 54-55 0.6180855240123968 1106 56-57 0.4381719818852936 1106 58-59 0.4829873524250985 1106 60-61 0.40485792265638665 1106 62-63 0.5712952976224308 1106 64-65 0.6964719789127045 1106 66-67 0.6783380236428975 1106 68-69 0.6547062939819455 1106 70-71 0.7037177720335492 1106 72-73 0.6355408002413583 1106 74-75 0.6567391886693628 1106 76-77 0.6965833525191982 1106 78-79 0.8326475968534837 1106 80-81 0.8284166225428891 1106 82-83 0.9042887101308814 1106 84-85 0.9397775785527749 1106 86-87 1.0050017702865368 1106 88-89 1.0422479571133572 1106 90-91 0.966759759153561 1106 92-93 1.1472063391982736 1106 94-95 1.1482164292336385 1106 96-97 1.3409670354068837 1106 98-99 1.4542674085880911 1106 100-101 1.6364484225385816 1107 1 0.04981412001712471 1107 2 0.16968875663039995 1107 3 0.1826803104641428 1107 4 0.09505606083244089 1107 5 0.18286969351066062 1107 6 0.2074459010375591 1107 7 0.2698346134334102 1107 8 0.2653540908370431 1107 9 0.25360934093059484 1107 10-11 0.24217051278965585 1107 12-13 0.25837872752569524 1107 14-15 0.29644250320075827 1107 16-17 0.24101737041318927 1107 18-19 0.2160171279127212 1107 20-21 0.2542894529629507 1107 22-23 0.4368883703010269 1107 24-25 0.49996809015783583 1107 26-27 0.490055611138672 1107 28-29 0.5114986455905282 1107 30-31 0.5102588071986389 1107 32-33 0.4824231416375824 1107 34-35 0.5346864902857007 1107 36-37 0.4280581620012285 1107 38-39 0.4875253704486653 1107 40-41 0.3662215894620644 1107 42-43 0.4137492871557029 1107 44-45 0.4225068504474976 1107 46-47 0.4450027395348499 1107 48-49 0.4047131609932677 1107 50-51 0.3327977990751805 1107 52-53 0.36148232830981897 1107 54-55 0.5760443605361125 1107 56-57 0.4010561684292391 1107 58-59 0.5113378921756642 1107 60-61 0.4324242364187434 1107 62-63 0.49904703463581157 1107 64-65 0.5415327272647872 1107 66-67 0.5742808281299467 1107 68-69 0.5933215509921546 1107 70-71 0.6187874743473962 1107 72-73 0.6119405956337687 1107 74-75 0.6052552410754082 1107 76-77 0.6260601858683295 1107 78-79 0.6050649161970512 1107 80-81 0.6979715344005726 1107 82-83 0.7276081504030962 1107 84-85 0.8366751407374586 1107 86-87 0.8912474684442735 1107 88-89 0.9199127054410674 1107 90-91 0.9208812562384523 1107 92-93 0.9297425966992527 1107 94-95 0.9386064781620398 1107 96-97 1.0622906616823968 1107 98-99 1.2312844771788463 1107 100-101 1.568559126515865 1108 1 0.1053641924730293 1108 2 0.14118729053639356 1108 3 -0.06783985184484465 1108 4 0.12683873940191148 1108 5 0.2186077979444505 1108 6 0.15982552702179476 1108 7 0.12452555300156831 1108 8 0.09922698057699364 1108 9 0.07197526729565595 1108 10-11 0.09610719587651317 1108 12-13 0.10950474232064522 1108 14-15 0.11249427511729237 1108 16-17 0.22211615586735434 1108 18-19 0.03986503605938907 1108 20-21 0.11237460212844042 1108 22-23 0.23430233818096724 1108 24-25 0.3143261385974867 1108 26-27 0.22620886982517163 1108 28-29 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2315 88-89 -1.970157465757989 2315 90-91 -2.0702114363822233 2315 92-93 -2.038764851095703 2315 94-95 -2.1901476260658974 2315 96-97 -2.13003329931977 2315 98-99 -2.2137138339891393 2315 100-101 -2.202429966100784 2316 1 -1.2695787808803907 2316 2 -1.0933357390139484 2316 3 -2.294398242945853 2316 4 -1.434829296441805 2316 5 -1.273606447394421 2316 6 -2.6562532190116777 2316 7 -6.782331714552601 2316 8 -7.646068393071271 2316 9 -4.305441353239033 2316 10-11 -2.177523989906831 2316 12-13 -2.0863186619833627 2316 14-15 -2.1318741059617636 2316 16-17 -2.10317996706555 2316 18-19 -1.669039830785259 2316 20-21 -2.650125334519288 2316 22-23 -3.197871967195887 2316 24-25 -2.0411359285173774 2316 26-27 -2.335530614906588 2316 28-29 -3.068345769234675 2316 30-31 -3.5204710273758764 2316 32-33 -3.472292681886394 2316 34-35 -2.688510596132211 2316 36-37 -3.73381224690295 2316 38-39 -3.2810597886848214 2316 40-41 -4.022309408812227 2316 42-43 -3.291123357505036 2316 44-45 -2.993757011571958 2316 46-47 -2.757747454756821 2316 48-49 -2.8389231822459386 2316 50-51 -2.4755928159696694 2316 52-53 -2.876326671853043 2316 54-55 -4.350667620867593 2316 56-57 -3.072864608536296 2316 58-59 -2.4993409932999846 2316 60-61 -3.191568686683535 2316 62-63 -2.9539099463443 2316 64-65 -4.399750984613334 2316 66-67 -3.3647853619853727 2316 68-69 -3.2247045287724596 2316 70-71 -3.554217689807306 2316 72-73 -3.1714417748290984 2316 74-75 -2.954516261664793 2316 76-77 -3.4507490139052877 2316 78-79 -4.5129437798301915 2316 80-81 -3.6165444568970138 2316 82-83 -3.1645594773739028 2316 84-85 -3.3330833650599594 2316 86-87 -4.409335812762027 2316 88-89 -4.697434581690114 2316 90-91 -4.228134664653794 2316 92-93 -4.372127235809526 2316 94-95 -4.5155838342558745 2316 96-97 -4.580466939677123 2316 98-99 -4.7343274052318485 2316 100-101 -4.849843302700652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 98151.0 3 17326.0 4 15345.0 5 14640.0 6 16871.0 7 20682.0 8 26383.0 9 31132.0 10 36505.0 11 44141.0 12 46807.0 13 53603.0 14 61756.0 15 73916.0 16 94300.0 17 125530.0 18 182588.0 19 265283.0 20 339220.0 21 244552.0 22 145253.0 23 127475.0 24 131305.0 25 139688.0 26 154435.0 27 174011.0 28 199769.0 29 232272.0 30 272996.0 31 324730.0 32 392280.0 33 484789.0 34 620582.0 35 854845.0 36 1216975.0 37 1348226.0 38 1046885.0 39 172910.0 40 225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.013222378942443 49.00256741471484 24.29599422712928 16.688215979213435 2 15.663374647819175 50.690445697749794 18.56386969586479 15.082309958566245 3 44.42029123244164 20.783917985965005 19.41041383817093 15.385376943422427 4 15.709330083241593 19.261356891620064 20.35327266784507 44.676040357293274 5 15.691224265715906 48.110824526680865 21.396636137751198 14.801315069852034 6 44.66133675020356 20.267121174364856 19.264093065268373 15.80744901016321 7 16.52540400423544 19.737933639208194 48.94556109118902 14.791101265367345 8 44.24031808844472 20.47916940230155 20.060866266989564 15.219646242264167 9 43.815505381491086 20.127035812411805 20.70419507822611 15.353263727870997 10-11 15.433391774482 34.32257502005199 34.517521115688375 15.726512089777636 12-13 15.288767226569059 20.445142851191353 34.46073354554805 29.805356376691538 14-15 15.478449386446103 48.74699708896543 20.103383314908925 15.671170209679545 16-17 29.692964320078453 20.260958049923776 20.220187006235353 29.82589062376242 18-19 15.597116015966817 34.386228811137656 20.225211872748076 29.791443300147453 20-21 29.844231170641333 34.402490090493 20.234530290460604 15.51874844840506 22-23 29.76032091987223 20.171735449772 34.46005843070938 15.607885199646384 24-25 15.572647945506624 34.174443897675786 20.677861517812627 29.57504663900496 26-27 15.767026739350984 25.411343178419493 42.994429822498525 15.827200259730997 28-29 26.423272286643634 20.09296837992165 37.78437890252898 15.699380430905737 30-31 24.01766490556654 21.238309839583184 38.232253933220676 16.511771321629595 32-33 18.458536379211136 23.18434113983102 39.92797628142403 18.42914619953382 34-35 17.97845859780782 22.541031804706485 40.79928001267577 18.681229584809916 36-37 17.963506836055263 22.629415899488414 40.64376938560281 18.763307878853514 38-39 18.504640406115794 22.940675014710806 40.091321802553345 18.463362776620055 40-41 18.505500176900703 23.086367419588917 39.947369183008284 18.460763220502095 42-43 18.89779545885146 22.97175966585209 39.891807270653004 18.238637604643447 44-45 18.51616127209223 22.8717132201013 40.066345895282026 18.54577961252445 46-47 18.701273277726706 22.90348647988459 39.82841966369961 18.56682057868909 48-49 18.846867153559923 23.111358445192405 39.55368727571803 18.48808712552963 50-51 18.998569206071913 23.481442448335883 38.837653652374776 18.682334693217424 52-53 19.09807879461429 24.056321631236656 37.84636452109039 18.99923505305867 54-55 19.912176877887045 24.791962179503916 36.16592049199072 19.129940450618328 56-57 20.187808951439386 25.608495923314013 34.7749797333951 19.428715391851505 58-59 20.467003341383634 26.378233127211416 33.85084431626997 19.303919215134982 60-61 20.499746087414806 26.537740634109987 33.366511109018454 19.596002169456757 62-63 20.04460214665737 26.608992317808024 33.5365686398814 19.809836895653206 64-65 20.19210167834738 26.250638369763884 33.70972809994237 19.847531851946368 66-67 19.978199857831786 25.911363527275196 33.91376804435404 20.19666857053898 68-69 20.052982593312908 25.98631790074061 34.04785560275154 19.91284390319495 70-71 19.718219786473774 25.842517931592813 34.5458638652715 19.893398416661917 72-73 19.702780986459402 25.56228681709472 34.55378139249547 20.181150803950406 74-75 20.06060386570723 25.521407644831704 34.0447195922006 20.373268897260463 76-77 20.114296574907016 25.825133127686318 34.00309897757713 20.05747131982953 78-79 20.26755658501875 26.17086101185326 33.11619215828278 20.445390244845218 80-81 20.291888478490595 26.25580659180617 33.07430933989898 20.37799558980425 82-83 20.678977639508396 26.448336749944207 32.637486463323725 20.23519914722368 84-85 20.70575864116689 26.5823702970982 32.56146008505964 20.150410976675264 86-87 20.72845636820247 26.601701871733813 32.41275773929016 20.257084020773565 88-89 20.703965674859383 26.550209996254477 32.19293933499103 20.55288499389511 90-91 20.947822524917736 26.68122185350324 32.1173987475293 20.253556874049718 92-93 20.89250869291854 26.826396648193658 31.98821335681073 20.292881302077074 94-95 20.68967076038885 26.980649970499844 31.695041942231704 20.6346373268796 96-97 20.572074743369495 26.800331673156187 32.12249032768087 20.50510325579345 98-99 20.591656216487227 26.781820771008924 31.86867796308549 20.75784504941836 100-101 20.984635038101366 26.550214996527004 31.428651084052333 21.036498881319297 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11497.0 1 7132.5 2 2045.0 3 1194.5 4 960.5 5 817.5 6 935.0 7 1168.5 8 1111.5 9 1100.0 10 1580.5 11 10873.5 12 193966.5 13 215850.5 14 54987.0 15 47456.0 16 51062.0 17 55977.0 18 60200.5 19 64692.0 20 69677.5 21 73941.0 22 78920.0 23 85718.5 24 93345.5 25 102077.0 26 114297.5 27 127397.5 28 138974.5 29 150817.0 30 161909.0 31 173696.5 32 188116.5 33 204328.0 34 227846.0 35 240915.0 36 246815.5 37 259225.0 38 266726.0 39 274742.0 40 286024.5 41 292193.5 42 293984.0 43 293725.0 44 298171.5 45 302391.0 46 295382.0 47 291966.0 48 290461.5 49 285874.5 50 280285.5 51 271390.0 52 265225.5 53 258757.5 54 252671.0 55 244785.0 56 230720.5 57 214590.0 58 207000.0 59 175959.5 60 123267.0 61 89817.5 62 66528.0 63 49126.0 64 35796.0 65 25275.0 66 16879.5 67 10736.5 68 6873.0 69 4778.5 70 3566.0 71 2928.5 72 2532.5 73 2190.0 74 1971.0 75 1819.5 76 1666.5 77 1592.5 78 1500.0 79 1336.0 80 1205.5 81 1120.0 82 1036.0 83 942.5 84 817.0 85 675.0 86 547.5 87 447.0 88 378.0 89 322.0 90 241.0 91 175.5 92 127.5 93 84.0 94 62.5 95 40.5 96 26.0 97 23.0 98 16.5 99 9.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12213173696958546 2 0.10916514001995455 3 0.10418970344570305 4 0.09209634638461424 5 0.09659454720582528 6 0.08710060190597806 7 0.09646254582732473 8 0.09246188866353884 9 0.0876996850853267 10-11 0.07831743325959534 12-13 0.07041258147785087 14-15 0.07841389580542266 16-17 0.06943780206738528 18-19 0.0618832616362769 20-21 0.05775060309399046 22-23 0.05003867640390065 24-25 0.04762203578212137 26-27 0.04417984598891473 28-29 0.04675894984577162 30-31 0.03836163138269819 32-33 0.05034329496967116 34-35 0.06078663479950311 36-37 0.05136884414109851 38-39 0.05359255967122315 40-41 0.053709330121435174 42-43 0.04911466675439682 44-45 0.04893189561493451 46-47 0.04844958288579789 48-49 0.04819065710489297 50-51 0.049713749933745464 52-53 0.04632232990150057 54-55 0.04690110517646452 56-57 0.04672848798919457 58-59 0.04929743789385911 60-61 0.045114009590610925 62-63 0.054404875846611155 64-65 0.05025190939994 66-67 0.0447738521921672 68-69 0.04270244594492781 70-71 0.050779914913942216 72-73 0.04497185425991802 74-75 0.04691633610475304 76-77 0.0423165957616185 78-79 0.04703818353106124 80-81 0.04902328118466567 82-83 0.03889979084889274 84-85 0.04892681863883834 86-87 0.051805464085369554 88-89 0.04706356841154212 90-91 0.04304768031946771 92-93 0.040803656884958364 94-95 0.044332155271799976 96-97 0.031959564525421535 98-99 0.03306126833829151 100-101 0.02446087083137108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9848382.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.46096275714101 #Duplication Level Percentage of deduplicated Percentage of total 1 89.61668333525829 35.36360603511127 2 5.488387315879359 4.33154094937361 3 1.7082205533500743 2.0222408291019027 4 0.8450621764875038 1.3338786829536766 5 0.532794729116177 1.0512296481427246 6 0.34508549377686193 0.8170443490775019 7 0.23820310155669924 0.657980660341507 8 0.15918158191525875 0.5025166780464653 9 0.11541150643218792 0.40988242413594966 >10 0.8440606566525579 6.795613194331411 >50 0.0672073132136523 1.730153334041942 >100 0.028368812552421317 2.077835694607407 >500 0.004829584009513178 1.4176105303495892 >1k 0.005602317451034589 4.715153344636585 >5k 4.507611742211738E-4 1.3003148716398287 >10k+ 4.507611742211738E-4 35.473398774108645 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1293659 13.135751639203272 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 33007 0.3351515000128955 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA 22357 0.2270119091643683 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 12921 0.13119921627735398 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCCGCAGCGGCTTCTGA 12764 0.12960504578315504 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 11229 0.11401872916789782 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA 10067 0.10221983672038717 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC 9882 0.10034135556480242 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0153952192349971E-5 0.0 0.0 0.0 0.0 2 1.0153952192349971E-5 0.0 0.0 1.0153952192349971E-5 0.0 3 1.0153952192349971E-5 0.0 0.0 2.0307904384699942E-5 0.0 4 1.0153952192349971E-5 0.0 0.0 2.0307904384699942E-5 0.0 5 1.0153952192349971E-5 0.0 0.0 2.0307904384699942E-5 0.0 6 2.0307904384699942E-5 0.0 0.0 2.0307904384699942E-5 0.0 7 2.0307904384699942E-5 0.0 0.0 2.0307904384699942E-5 0.0 8 2.0307904384699942E-5 0.0 0.0 2.0307904384699942E-5 0.0 9 5.076976096174986E-5 0.0 0.0 2.0307904384699942E-5 0.0 10-11 5.5846737057924846E-5 0.0 0.0 2.0307904384699942E-5 0.0 12-13 7.107766534644981E-5 0.0 0.0 2.538488048087493E-5 0.0 14-15 8.630859363497476E-5 0.0 0.0 3.55388326732249E-5 0.0 16-17 1.0153952192349972E-4 0.0 0.0 4.0615808769399884E-5 0.0 18-19 1.066164980196747E-4 0.0 0.0 5.076976096174986E-5 0.0 20-21 1.1169347411584969E-4 0.0 0.0 5.076976096174986E-5 0.0 22-23 1.3707835459672463E-4 0.0 0.0 5.076976096174986E-5 1.0153952192349971E-5 24-25 1.6754021117377452E-4 0.0 0.0 5.076976096174986E-5 1.5230928288524957E-5 26-27 1.8277113946229949E-4 0.0 0.0 5.076976096174986E-5 2.0307904384699942E-5 28-29 2.0307904384699943E-4 0.0 0.0 5.076976096174986E-5 2.0307904384699942E-5 30-31 2.3354090042404936E-4 0.0 0.0 6.092371315409983E-5 2.0307904384699942E-5 32-33 2.640027570010993E-4 0.0 0.0 6.600068925027482E-5 2.538488048087493E-5 34-35 2.995415896743242E-4 0.0 0.0 7.107766534644981E-5 3.55388326732249E-5 36-37 3.0969554186667417E-4 0.0 0.0 7.107766534644981E-5 4.0615808769399884E-5 38-39 3.249264701551991E-4 0.0 0.0 7.107766534644981E-5 4.0615808769399884E-5 40-41 4.010811115978239E-4 0.0 0.0 7.107766534644981E-5 4.0615808769399884E-5 42-43 4.0615808769399887E-4 0.0 0.0 7.615464144262478E-5 4.0615808769399884E-5 44-45 4.1631203988634884E-4 1.0153952192349971E-5 0.0 8.123161753879977E-5 4.0615808769399884E-5 46-47 4.4677389646339877E-4 1.0153952192349971E-5 0.0 8.123161753879977E-5 4.0615808769399884E-5 48-49 4.721587769442737E-4 1.0153952192349971E-5 0.0 8.630859363497476E-5 4.0615808769399884E-5 50-51 4.975436574251486E-4 1.0153952192349971E-5 0.0 1.0153952192349972E-4 4.569278486557487E-5 52-53 5.127745857136737E-4 1.0153952192349971E-5 0.0 1.1169347411584969E-4 5.076976096174986E-5 54-55 5.432364422907235E-4 1.0153952192349971E-5 0.0 1.2184742630819967E-4 5.5846737057924846E-5 56-57 5.736982988677735E-4 1.0153952192349971E-5 0.0 1.3200137850054964E-4 6.092371315409983E-5 58-59 6.092371315409984E-4 1.0153952192349971E-5 0.0 1.4215533069289961E-4 6.092371315409983E-5 60-61 6.600068925027481E-4 1.0153952192349971E-5 0.0 1.6754021117377452E-4 6.092371315409983E-5 62-63 7.46315486137723E-4 1.0153952192349971E-5 0.0 1.726171872699495E-4 6.092371315409983E-5 64-65 8.173931514841727E-4 1.0153952192349971E-5 0.0 1.726171872699495E-4 6.092371315409983E-5 66-67 8.986247690229725E-4 1.0153952192349971E-5 0.0 2.0307904384699943E-4 7.107766534644981E-5 68-69 0.001015395219234997 1.0153952192349971E-5 0.0 2.0307904384699943E-4 8.123161753879977E-5 70-71 0.0011575505499278969 1.0153952192349971E-5 0.0 2.0815601994317442E-4 8.123161753879977E-5 72-73 0.0013047828567169712 1.0153952192349971E-5 0.0 2.2338694823169938E-4 8.123161753879977E-5 74-75 0.0014063223786404712 1.0153952192349971E-5 0.0 2.3861787652022434E-4 8.123161753879977E-5 76-77 0.0016144783985836456 1.0153952192349971E-5 0.0 2.487718287125743E-4 8.123161753879977E-5 78-79 0.0018378653468153448 1.0153952192349971E-5 0.0 2.6907973309727427E-4 8.123161753879977E-5 80-81 0.0021120220560087942 1.0153952192349971E-5 0.0 2.8431066138579923E-4 1.0153952192349972E-4 82-83 0.002457256430548693 1.0153952192349971E-5 0.0 2.944646135781492E-4 1.0153952192349972E-4 84-85 0.0030157238011279414 1.0153952192349971E-5 0.0 3.046185657704992E-4 1.0153952192349972E-4 86-87 0.0037366544067847897 1.0153952192349971E-5 0.0 3.198494940590241E-4 1.0153952192349972E-4 88-89 0.004929743789385911 1.0153952192349971E-5 0.0 3.249264701551991E-4 1.0153952192349972E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 281630 0.0 91.734535 5 AAGCAGT 283375 0.0 91.681725 1 TGGTATC 282000 0.0 91.57324 7 GTATCAA 283175 0.0 91.565346 9 GTGGTAT 282655 0.0 91.52707 6 GGTATCA 283065 0.0 91.49322 8 GCAGTGG 285070 0.0 90.86318 3 AGCAGTG 286475 0.0 90.63939 2 CAGTGGT 285805 0.0 90.39568 4 TCAACGC 281785 0.0 46.396427 12-13 ATCAACG 281720 0.0 46.34854 10-11 CAACGCA 281935 0.0 46.336353 12-13 AACGCAG 283460 0.0 46.17722 14-15 ACGCAGA 283350 0.0 46.165802 14-15 CGCAGAG 283480 0.0 46.14224 16-17 TATCAAC 283615 0.0 46.029644 10-11 AGAGTAC 276080 0.0 45.91003 18-19 CAGAGTA 282505 0.0 45.74783 18-19 GAGTACT 167620 0.0 45.630283 20-21 GCAGAGT 286405 0.0 45.456207 16-17 >>END_MODULE