FastQCFastQC Report
Sat 18 Jun 2016
SRR578585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9848382
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1363521.3845116893313034No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131180.13319954485924693No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111190.11290179442673934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4850.024.4718423
CGCGTAG2400.023.73901254-55
ACTCGCG2600.022.8292250-51
CTCGCGT2750.020.71957652-53
CGTACGC3050.020.23571220-21
CAACGCA72450.020.08427212-13
ATCAACG74700.019.38399110-11
TCAACGC68800.018.04459810-11
GTATCAA96300.017.1560369
ACGCAGA86050.016.99275614-15
CGTAGCG3500.016.9556256-57
AGTGGTA101250.016.5986295
TGGTATC101800.016.1825037
AACGCAG79350.015.88479412-13
GGTATCA103150.015.8786598
GTGGTAT106600.015.58744056
AAGCAGT113150.015.3807241
ACGTACG3900.015.21669920-21
CGCAGAG83200.014.97855714-15
AGCGACG4400.014.56656758-59
Produced by FastQC (version 0.11.5)