##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578585_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9848382 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.427884296120926 31.0 31.0 34.0 30.0 34.0 2 31.805114078637487 31.0 31.0 34.0 30.0 34.0 3 31.882840551879486 31.0 31.0 34.0 30.0 34.0 4 35.579565861681644 37.0 35.0 37.0 33.0 37.0 5 35.40402179769225 37.0 35.0 37.0 33.0 37.0 6 35.39767293754446 37.0 35.0 37.0 33.0 37.0 7 35.38776511715326 37.0 35.0 37.0 33.0 37.0 8 35.345023883110954 37.0 35.0 37.0 32.0 37.0 9 36.894395952553424 39.0 37.0 39.0 33.0 39.0 10-11 36.89063233940357 39.0 37.0 39.0 33.0 39.0 12-13 36.88270636740127 39.0 37.0 39.0 33.0 39.0 14-15 38.064571622018725 40.0 37.0 41.0 33.0 41.0 16-17 37.998164571601706 40.0 37.0 41.0 33.0 41.0 18-19 37.93211331566952 40.0 37.0 41.0 33.0 41.0 20-21 37.88657502318655 40.0 37.0 41.0 32.0 41.0 22-23 37.686271866789895 40.0 37.0 41.0 32.0 41.0 24-25 37.69130685629376 39.5 37.0 41.0 32.0 41.0 26-27 37.52380294549907 39.0 36.0 41.0 32.0 41.0 28-29 37.37458919647918 39.0 36.0 41.0 31.5 41.0 30-31 37.235670336508065 39.0 36.0 41.0 31.0 41.0 32-33 37.09891081601018 39.0 36.0 41.0 31.0 41.0 34-35 36.95042683153436 39.0 36.0 40.0 30.5 41.0 36-37 36.80043686363912 39.0 35.0 40.0 30.0 41.0 38-39 36.63068669554045 39.0 35.0 40.0 30.0 41.0 40-41 36.47041260178575 38.0 35.0 40.0 30.0 41.0 42-43 36.55417905194986 39.0 35.0 40.0 30.0 41.0 44-45 36.48759872433868 39.0 35.0 40.0 30.0 41.0 46-47 36.33827373877252 38.0 35.0 40.0 30.0 41.0 48-49 36.19537458031177 38.0 35.0 40.0 29.5 41.0 50-51 36.01045364609131 38.0 35.0 40.0 29.0 41.0 52-53 36.05006954441856 38.0 35.0 40.0 29.0 41.0 54-55 36.03998575603586 38.0 35.0 40.0 29.0 41.0 56-57 35.88623237806982 38.0 34.5 40.0 28.5 41.0 58-59 35.668286932818 38.0 34.0 40.0 28.0 41.0 60-61 35.41517606648483 38.0 34.0 40.0 28.0 41.0 62-63 35.14117400198327 37.5 34.0 40.0 28.0 41.0 64-65 34.8265463809182 37.0 33.5 40.0 27.0 41.0 66-67 34.493034439565804 36.5 33.0 39.5 26.0 41.0 68-69 34.11917470301213 36.0 33.0 39.0 26.0 41.0 70-71 33.73029056955752 35.5 32.0 39.0 26.0 40.0 72-73 33.342083806253655 35.0 32.0 38.5 26.0 40.0 74-75 32.930905147667914 35.0 32.0 37.5 25.5 40.0 76-77 31.55010102167036 34.0 30.5 36.0 23.0 39.0 78-79 32.20271934009058 35.0 31.0 36.0 25.0 39.0 80-81 32.07407059352491 35.0 31.0 36.0 25.0 39.0 82-83 31.704216946499436 34.5 31.0 36.0 24.0 37.0 84-85 31.417204978442143 34.0 31.0 35.0 23.5 37.0 86-87 31.095894838360252 34.0 31.0 35.0 22.5 36.5 88-89 30.78448840631893 34.0 31.0 35.0 20.5 36.0 90-91 30.442077744344196 34.0 30.5 35.0 20.0 36.0 92-93 30.072498863265054 34.0 30.0 35.0 18.0 35.5 94-95 29.691994177317653 34.0 30.0 35.0 13.5 35.0 96-97 29.230243556758865 34.0 29.5 35.0 4.5 35.0 98-99 28.415949696102366 34.0 29.0 35.0 2.0 35.0 100-101 27.36974637052056 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3444452684076005 1101 2 0.20752240712876002 1101 3 0.3278029531349631 1101 4 0.30537066614741804 1101 5 0.2982445874957449 1101 6 0.23252550647890757 1101 7 0.19453793999103652 1101 8 0.21257507473349335 1101 9 0.30911101404443997 1101 10-11 0.33532224326968674 1101 12-13 0.28893684396061303 1101 14-15 0.2735709384613543 1101 16-17 0.32862245064150386 1101 18-19 0.36547594280531115 1101 20-21 0.3262582461876633 1101 22-23 0.43105122649636485 1101 24-25 0.4010368195035099 1101 26-27 0.35887567316239455 1101 28-29 0.38146022933021584 1101 30-31 0.4319764752949169 1101 32-33 0.4351381619473784 1101 34-35 0.44212084166549204 1101 36-37 0.4396282404967877 1101 38-39 0.3357539746414915 1101 40-41 0.35222606160699854 1101 42-43 0.33826021775240633 1101 44-45 0.3621541240247268 1101 46-47 0.38805991675739193 1101 48-49 0.3560240086874913 1101 50-51 0.4167070743951484 1101 52-53 0.41955462211723216 1101 54-55 0.4712265116812233 1101 56-57 0.5743884805170083 1101 58-59 0.5330220867971818 1101 60-61 0.4794673909014975 1101 62-63 0.36637606404971024 1101 64-65 0.39579678676469854 1101 66-67 0.519585360189545 1101 68-69 0.3613069019824948 1101 70-71 0.31251435263754956 1101 72-73 0.4532161232898346 1101 74-75 0.44756201771325266 1101 76-77 0.3147300965960014 1101 78-79 0.3783772665614933 1101 80-81 0.3668768322241718 1101 82-83 0.41993842856355457 1101 84-85 0.3505109472338539 1101 86-87 0.34221240560057353 1101 88-89 0.4191035679186008 1101 90-91 0.41007971595184145 1101 92-93 0.4444321088045875 1101 94-95 0.25003955120315524 1101 96-97 0.30109667578343746 1101 98-99 0.7953914992406261 1101 100-101 0.9249128208457478 1102 1 0.24978936910419947 1102 2 0.2862600974238454 1102 3 0.2833204854288951 1102 4 0.27932266721435184 1102 5 0.25072053522128357 1102 6 0.18622010661435695 1102 7 0.26616438184379376 1102 8 0.09041927707867359 1102 9 0.1938781734247712 1102 10-11 0.23631855970597826 1102 12-13 0.2960001562062686 1102 14-15 0.33488327653536487 1102 16-17 0.21696133973618004 1102 18-19 0.3251971424320672 1102 20-21 0.2989521687618151 1102 22-23 0.472737052910027 1102 24-25 0.39318711312915866 1102 26-27 0.41649980190865676 1102 28-29 0.362684780441235 1102 30-31 0.40206518274800374 1102 32-33 0.35414128310355864 1102 34-35 0.4025027879071459 1102 36-37 0.39689552619270074 1102 38-39 0.3511847946312372 1102 40-41 0.3478405060081897 1102 42-43 0.2686777757370109 1102 44-45 0.35755280415129675 1102 46-47 0.32706784880014084 1102 48-49 0.26302565960846636 1102 50-51 0.3518623093546651 1102 52-53 0.3554819135136853 1102 54-55 0.4088933505098211 1102 56-57 0.4549810179529814 1102 58-59 0.47784495494481405 1102 60-61 0.4894894168849788 1102 62-63 0.34935331832439687 1102 64-65 0.325361647789407 1102 66-67 0.4033307611822039 1102 68-69 0.3478209102259626 1102 70-71 0.4108758206675347 1102 72-73 0.48901715460874584 1102 74-75 0.399819734464522 1102 76-77 0.3088115488276024 1102 78-79 0.4264207646721445 1102 80-81 0.45678198104447887 1102 82-83 0.5593644538647453 1102 84-85 0.4339848550398848 1102 86-87 0.45876410830373615 1102 88-89 0.4524654546708611 1102 90-91 0.45293558023633196 1102 92-93 0.5127902147229442 1102 94-95 0.5013105387574655 1102 96-97 0.5975462028533904 1102 98-99 0.9544834211733644 1102 100-101 1.0355241043768828 1103 1 0.24239795355471827 1103 2 0.28536920985437675 1103 3 0.2756524257377819 1103 4 0.2432177224826475 1103 5 0.24881234061557223 1103 6 0.05858222753978026 1103 7 0.2571137920392914 1103 8 0.10502956104230776 1103 9 0.23820146001241937 1103 10-11 0.22127103784845303 1103 12-13 0.2655065479046357 1103 14-15 0.39012486649252764 1103 16-17 0.31215650831889263 1103 18-19 0.26088649941602426 1103 20-21 0.42615749176884066 1103 22-23 0.40381426254315045 1103 24-25 0.39145365931275933 1103 26-27 0.2797100491813964 1103 28-29 0.38704571419361855 1103 30-31 0.433508104968098 1103 32-33 0.3758446050512845 1103 34-35 0.37636242500690287 1103 36-37 0.5025793567935395 1103 38-39 0.48596820704850785 1103 40-41 0.3522346691499081 1103 42-43 0.40178200696243493 1103 44-45 0.4107315875199191 1103 46-47 0.4950437138939989 1103 48-49 0.39462116468144615 1103 50-51 0.3761545390828829 1103 52-53 0.2537878215682241 1103 54-55 0.4638346739217667 1103 56-57 0.32688238013898285 1103 58-59 0.32447287409907233 1103 60-61 0.3390760166977387 1103 62-63 0.47214620789560513 1103 64-65 0.3407326625193079 1103 66-67 0.2828337742472016 1103 68-69 0.5060857839515265 1103 70-71 0.35899981387176183 1103 72-73 0.2927176417055506 1103 74-75 0.3407553266710721 1103 76-77 0.25946748589930735 1103 78-79 0.3449739060618455 1103 80-81 0.29606402328109027 1103 82-83 0.571498854007217 1103 84-85 0.3613880293871965 1103 86-87 0.32467338275592894 1103 88-89 0.3500766548506391 1103 90-91 0.46015726180860383 1103 92-93 0.4375139321279846 1103 94-95 0.5406082859316932 1103 96-97 0.5685433855517026 1103 98-99 1.0665403142394752 1103 100-101 0.9234567508311109 1104 1 0.25980745627104795 1104 2 0.2169824071455757 1104 3 0.1599824547256219 1104 4 0.1268647616890064 1104 5 0.19537579466154398 1104 6 0.1388704551234241 1104 7 0.16600783015439902 1104 8 0.06981309951856929 1104 9 0.1962241099249269 1104 10-11 0.10387972326416417 1104 12-13 0.16775129340809514 1104 14-15 0.28092757941079327 1104 16-17 0.32258294948253763 1104 18-19 0.12138285399441173 1104 20-21 0.1538596095377187 1104 22-23 0.22955532851607785 1104 24-25 0.18414037226997237 1104 26-27 0.258095522749862 1104 28-29 0.2161243211912378 1104 30-31 0.15395843464951042 1104 32-33 0.1739167896776337 1104 34-35 0.18063379890116238 1104 36-37 0.12130698368964232 1104 38-39 0.13822316148934277 1104 40-41 0.20283115401493035 1104 42-43 0.15715009771783173 1104 44-45 0.1308564578604816 1104 46-47 0.17508307290885483 1104 48-49 0.17989827639421208 1104 50-51 0.14413972892203475 1104 52-53 0.17009409230117 1104 54-55 0.17701406386574803 1104 56-57 0.13590623201537966 1104 58-59 0.13750872954906868 1104 60-61 0.2270980109432159 1104 62-63 0.12820558418334116 1104 64-65 0.1959569319016694 1104 66-67 0.19526279542299108 1104 68-69 0.16892249983292373 1104 70-71 0.15634873683282535 1104 72-73 0.2138498809204208 1104 74-75 0.21246731679894992 1104 76-77 0.12423860548023313 1104 78-79 0.2486213021853061 1104 80-81 0.32645076711043686 1104 82-83 0.4163543647006094 1104 84-85 0.27532914059600344 1104 86-87 0.3864113281178554 1104 88-89 0.37166291874914137 1104 90-91 0.4033493033167197 1104 92-93 0.46571686356499953 1104 94-95 0.4178766583983631 1104 96-97 0.3250151494906781 1104 98-99 0.7881036060503952 1104 100-101 0.8333943461293671 1105 1 0.18536797076603406 1105 2 0.1476800819434203 1105 3 0.07278263809486774 1105 4 0.11469935096751982 1105 5 0.14417409981580676 1105 6 -0.0038687636235650302 1105 7 0.16352992585034087 1105 8 0.0395818630240683 1105 9 0.2010740636777797 1105 10-11 0.040220571119739645 1105 12-13 0.09814469593811026 1105 14-15 0.1334352975238957 1105 16-17 0.10499412758838389 1105 18-19 0.20664366376613685 1105 20-21 0.1363333828003519 1105 22-23 0.20617718654914086 1105 24-25 0.1894506263563187 1105 26-27 0.15866463504830364 1105 28-29 0.24636445556138398 1105 30-31 0.16974134616602043 1105 32-33 0.21720254657074634 1105 34-35 0.14058210930692638 1105 36-37 0.13722254797888667 1105 38-39 0.18542313512470798 1105 40-41 0.22923547913528353 1105 42-43 0.21489165532145194 1105 44-45 0.2302386561210028 1105 46-47 0.21914379826974084 1105 48-49 0.2399276925720244 1105 50-51 0.2706246857667125 1105 52-53 0.23257108321009667 1105 54-55 0.3240137769910305 1105 56-57 0.2265198463965561 1105 58-59 0.15664138548844164 1105 60-61 0.09001669391654588 1105 62-63 0.12137590761302164 1105 64-65 0.1542992587102745 1105 66-67 0.2587300056466759 1105 68-69 0.18605758505825776 1105 70-71 0.21159369369040348 1105 72-73 0.29941688759370066 1105 74-75 0.3626826093621389 1105 76-77 0.16182081459834308 1105 78-79 0.35977790238865026 1105 80-81 0.45217644172632987 1105 82-83 0.4136294625739154 1105 84-85 0.3966955624726438 1105 86-87 0.3754720560386069 1105 88-89 0.39800312417525063 1105 90-91 0.551560269985913 1105 92-93 0.6926117304189354 1105 94-95 0.6623002028163611 1105 96-97 0.7076433665515189 1105 98-99 1.2712949880070035 1105 100-101 1.2807808565933918 1106 1 0.19571718573846297 1106 2 0.1854515296924184 1106 3 0.11925546150332167 1106 4 0.12410070227801384 1106 5 0.13692709315065343 1106 6 0.015052047095707621 1106 7 0.14317217769632862 1106 8 0.0647367459056909 1106 9 0.10972593218483695 1106 10-11 0.00725964355589781 1106 12-13 0.12621216774544308 1106 14-15 0.08463335690292695 1106 16-17 0.06955609544029073 1106 18-19 0.14436866511992008 1106 20-21 0.20721384068734494 1106 22-23 0.22421618027195223 1106 24-25 0.14884297532780977 1106 26-27 0.12345042374420956 1106 28-29 0.25326467320169144 1106 30-31 0.29355001664468716 1106 32-33 0.23342053733398416 1106 34-35 0.21577018069745435 1106 36-37 0.19701775733314975 1106 38-39 0.1719802276997484 1106 40-41 0.24876410006148575 1106 42-43 0.2449891183983368 1106 44-45 0.1097184193655778 1106 46-47 0.1119416585919808 1106 48-49 0.16967004879031578 1106 50-51 0.1749682600730651 1106 52-53 0.19749876156398471 1106 54-55 0.19321186184622263 1106 56-57 0.2344897058496329 1106 58-59 0.19124979059613167 1106 60-61 0.28982097586919764 1106 62-63 0.3014521814915909 1106 64-65 0.22961717189497932 1106 66-67 0.18566716124723115 1106 68-69 0.28357166187563365 1106 70-71 0.27303043519859216 1106 72-73 0.32516454346307455 1106 74-75 0.3538869262045665 1106 76-77 0.21841909877689858 1106 78-79 0.34971669371876857 1106 80-81 0.42636965564589246 1106 82-83 0.5703220275939174 1106 84-85 0.5508398971948374 1106 86-87 0.5735629708658898 1106 88-89 0.621014990545202 1106 90-91 0.537168427872011 1106 92-93 0.5929227642781605 1106 94-95 0.6075586748589537 1106 96-97 0.6508521975490531 1106 98-99 1.1194438030303395 1106 100-101 1.2022414369731216 1107 1 0.14356862516848423 1107 2 0.0660661691486375 1107 3 0.038250082082644354 1107 4 -0.00593774064831365 1107 5 -0.00904489951941656 1107 6 -0.008288314626447857 1107 7 -0.04236644588927874 1107 8 0.008440998080935458 1107 9 0.07138643282998913 1107 10-11 0.015244184527098525 1107 12-13 -9.366260254140002E-4 1107 14-15 0.02128742310760856 1107 16-17 0.03164893623053189 1107 18-19 -0.030287239849869252 1107 20-21 -0.025709492192916628 1107 22-23 0.12944734706445615 1107 24-25 0.11995875299466263 1107 26-27 0.11207670782437873 1107 28-29 0.0843506972923933 1107 30-31 0.05128149659265091 1107 32-33 0.07642452535450417 1107 34-35 0.10293499013331342 1107 36-37 0.13038142581707035 1107 38-39 0.15675456635158724 1107 40-41 0.15886272594674722 1107 42-43 0.05212657384745256 1107 44-45 0.056148109206517915 1107 46-47 0.044938716263224876 1107 48-49 0.013357743703416247 1107 50-51 0.09082871324043396 1107 52-53 0.07057490669450317 1107 54-55 0.07570166954021573 1107 56-57 0.1783538671964635 1107 58-59 0.11644189100690028 1107 60-61 0.08943551385923598 1107 62-63 0.1790184286251204 1107 64-65 0.23037519847873256 1107 66-67 0.08709283717758609 1107 68-69 0.18454928753663324 1107 70-71 0.18063324537384773 1107 72-73 0.09060803346551438 1107 74-75 0.21231053207262107 1107 76-77 0.17219826649534653 1107 78-79 0.15928231758727662 1107 80-81 0.19794834656849503 1107 82-83 0.3326104572896611 1107 84-85 0.38495883668482733 1107 86-87 0.39959394241691015 1107 88-89 0.35869942864150417 1107 90-91 0.41074931963977335 1107 92-93 0.4075618207226732 1107 94-95 0.4099864054317983 1107 96-97 0.4896604354223193 1107 98-99 0.959822044379731 1107 100-101 1.030966121352396 1108 1 0.07706492712495461 1108 2 -0.00123577027498456 1108 3 -0.060531325581010265 1108 4 -0.037568980347579384 1108 5 0.010821890260622524 1108 6 -0.024968624372156967 1108 7 -0.05169900484450096 1108 8 0.02043002874051325 1108 9 -0.01213016398605049 1108 10-11 -0.019791866044720052 1108 12-13 -0.10405927668234938 1108 14-15 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-1.165441882429814 2316 90-91 -1.175628551650746 2316 92-93 -1.2083568181640594 2316 94-95 -1.3717574692064538 2316 96-97 -1.4787729121855016 2316 98-99 -1.0638893298745806 2316 100-101 -1.4744012901022465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 129.0 3 114.0 4 230.0 5 653.0 6 1314.0 7 2319.0 8 4292.0 9 6685.0 10 9187.0 11 11658.0 12 14521.0 13 17460.0 14 20072.0 15 22879.0 16 26609.0 17 30638.0 18 36206.0 19 40900.0 20 44007.0 21 46009.0 22 51347.0 23 61381.0 24 75887.0 25 96062.0 26 121549.0 27 155304.0 28 193802.0 29 241685.0 30 295626.0 31 359697.0 32 439487.0 33 543927.0 34 702806.0 35 978443.0 36 1266315.0 37 1796298.0 38 1728648.0 39 403425.0 40 811.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.614923935897394 31.3014585907121 29.647776171168104 22.4358413022224 2 21.671163852092658 30.246684176141827 28.330887246250196 19.75126472551532 3 20.82251683576043 29.18899774602569 28.749392539810092 21.23909287840378 4 21.020707831592915 29.173513446935985 28.832845618174012 20.972933103297088 5 21.580968766639902 29.193275276707205 29.013599888549475 20.212156068103422 6 21.569075419868845 29.29503177834552 28.03577809248289 21.100114709302744 7 21.855642404732645 29.346816318610514 28.47325997224107 20.32428130441577 8 21.057268194640315 29.462324822729748 28.454788660186885 21.025618322443048 9 20.71521095832436 29.13852621454907 29.083583095552797 21.062679731573773 10-11 20.78284245352866 29.562821890702658 28.43267877897775 21.221656876790938 12-13 20.81694739298293 29.642376788390212 28.29362224170427 21.247053576922585 14-15 20.97712598881725 29.698416450539796 28.126848653921023 21.19760890672194 16-17 21.14100784687351 29.47084906646077 28.1337257362403 21.254417350425413 18-19 21.115342658080415 29.508183654339547 28.105597858896914 21.27087582868312 20-21 21.204692364933265 29.44272112123195 28.121052174918287 21.231534338916504 22-23 21.244410083229486 29.385596294512574 28.11368302368131 21.25631059857663 24-25 21.169370765675012 29.366473599419677 28.084247747498015 21.379907887407292 26-27 21.333576757661064 29.274645596246767 28.207261057969784 21.18451658812238 28-29 21.346408119816296 29.316358593063697 28.098052064169803 21.2391812229502 30-31 21.301498246188736 29.32991036436809 28.073957494434648 21.29463389500853 32-33 21.349074557139733 29.365653144897337 27.96498424205599 21.32028805590694 34-35 21.341655722300736 29.36517735965234 28.018837577350958 21.274329340695964 36-37 21.402992770451608 29.381231321077383 27.94091142125271 21.27486448721829 38-39 21.404191856177675 29.33524568420064 27.95643901652703 21.304123443094657 40-41 21.391106498361555 29.374226434487095 27.922753774928623 21.311913292222727 42-43 21.401250789670073 29.37054810248939 27.92146956743672 21.30673154040382 44-45 21.385156438008146 29.389016998855645 27.890643166187907 21.33518339694831 46-47 21.420954524507746 29.421008954798282 27.866744900054407 21.29129162063956 48-49 21.42371630756554 29.400324336735235 27.84699316266804 21.32896619303118 50-51 21.458446442827114 29.40361101101596 27.812549171372098 21.325393374784827 52-53 21.426837739466713 29.45490855986871 27.767521285930165 21.350732414734413 54-55 21.42167282661798 29.490388211480976 27.711002237972913 21.376936723928132 56-57 21.487468869606698 29.489525266483984 27.659126934021614 21.3638789298877 58-59 21.44034908962709 29.54788033542425 27.648516443717458 21.363254131231205 60-61 21.391452931049994 29.557758827795265 27.6980218679576 21.35276637319714 62-63 21.416132462495707 29.56247847042747 27.66049923477204 21.360889832304782 64-65 21.386225747733324 29.606818164856293 27.660270237022594 21.346685850387793 66-67 21.40323701687556 29.615480493264553 27.62734349654482 21.353938993315065 68-69 21.373194162628874 29.64423839223769 27.62271088400577 21.359856561127668 70-71 21.431343879419646 29.68526187830908 27.559080383602176 21.324313858669093 72-73 21.39853866060644 29.671429652161418 27.566917128317296 21.363114558914845 74-75 21.430883317662897 29.732209407816878 27.526528682536327 21.310378591983895 76-77 21.390558788850136 29.73932467851069 27.580159598254628 21.28995693438454 78-79 21.381033848389 29.75906263703296 27.527980046350493 21.331923468227544 80-81 21.38481391067751 29.8279757260824 27.497747464968363 21.289462898271726 82-83 21.382557756982017 29.846225081639616 27.498111322318074 21.273105839060293 84-85 21.35648049993217 29.878179915145402 27.49120768744004 21.274131897482388 86-87 21.342291658522196 29.91228849550603 27.473085169414297 21.27233467655748 88-89 21.341511615055115 29.979751452031028 27.44250172401792 21.236235208895938 90-91 21.353080630679457 29.962747254783643 27.424615547861592 21.259556566675304 92-93 21.360187546248756 30.03040899450282 27.3732330277372 21.236170431511223 94-95 21.33609449076187 30.08245445362038 27.376409832960263 21.205041222657485 96-97 21.31988712954641 30.099936725001207 27.345176922491504 21.23499922296088 98-99 21.386624687672597 30.125886451428364 27.255524072784524 21.231964788114507 100-101 21.376184144458644 30.110328070017783 27.24648938832932 21.266998397194254 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 145538.0 1 83676.5 2 15579.5 3 8224.5 4 6524.5 5 5684.0 6 5582.0 7 5483.0 8 4894.0 9 4560.5 10 4603.5 11 5451.0 12 9475.0 13 9321.5 14 5998.5 15 6127.0 16 6774.0 17 8111.5 18 9826.0 19 12342.5 20 16454.0 21 21736.5 22 28679.0 23 38703.0 24 49366.0 25 62465.5 26 83860.5 27 106825.5 28 120364.0 29 130596.0 30 148066.5 31 171586.0 32 197334.0 33 224090.5 34 250110.0 35 269783.0 36 285489.0 37 300751.5 38 311279.5 39 315035.0 40 320542.0 41 330623.5 42 337119.5 43 340036.5 44 344038.5 45 349746.0 46 349529.5 47 346005.5 48 341789.0 49 335675.5 50 330106.5 51 319440.0 52 309083.5 53 298905.5 54 282876.5 55 262569.5 56 238379.5 57 213896.5 58 189102.5 59 158114.5 60 123423.0 61 92921.5 62 67786.5 63 48289.5 64 34261.5 65 23147.0 66 14494.0 67 9196.0 68 5717.5 69 3461.5 70 2018.5 71 1071.5 72 559.0 73 311.5 74 174.5 75 100.5 76 72.5 77 59.5 78 39.5 79 29.5 80 20.0 81 9.5 82 8.0 83 8.5 84 9.5 85 10.0 86 6.5 87 3.0 88 2.5 89 2.5 90 0.5 91 1.5 92 2.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1633060131095646 2 0.0 3 0.0 4 8.021622231956478E-4 5 6.701608446950982E-4 6 9.138556973114974E-5 7 1.929250916546495E-4 8 1.0153952192349973E-5 9 1.523092828852496E-4 10-11 2.7923368528962424E-4 12-13 0.0 14-15 0.0 16-17 1.0153952192349973E-5 18-19 5.076976096174986E-6 20-21 5.076976096174986E-6 22-23 7.006227012721481E-4 24-25 0.0 26-27 1.0153952192349972E-4 28-29 0.005818214606216534 30-31 0.004071734829132339 32-33 1.6246323507759956E-4 34-35 8.935477929267976E-4 36-37 4.975436574251486E-4 38-39 0.0012133972869858216 40-41 0.003198494940590241 42-43 0.0012540130957552215 44-45 7.310845578491979E-4 46-47 0.009382251825731375 48-49 0.009143633949211149 50-51 0.008199316395322603 52-53 0.015251236192909658 54-55 0.006407143833372833 56-57 8.986247690229725E-4 58-59 0.001396168426448121 60-61 0.0 62-63 9.646254582732474E-5 64-65 0.001030626147523522 66-67 0.0011880124065049467 68-69 0.002888799398723567 70-71 0.0021374069364896693 72-73 0.005757290893062434 74-75 0.002238946458413169 76-77 0.0015637086376218954 78-79 0.0016094014224874705 80-81 3.4015739844372404E-4 82-83 0.002254177386701694 84-85 6.0923713154099826E-5 86-87 0.001898789059969445 88-89 2.183099721355244E-4 90-91 0.0011626275260240718 92-93 0.001467246091794571 94-95 9.798563865617723E-4 96-97 0.0010204721953311722 98-99 0.5317777072416566 100-101 2.7415670919344926E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9848382.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.20927028681453 #Duplication Level Percentage of deduplicated Percentage of total 1 89.56511685151398 53.92650328782932 2 5.757484314220544 6.933078584939993 3 1.3945698329200429 2.518980960123619 4 0.732599121430744 1.764370340564261 5 0.48891140422541635 1.4718499441657065 6 0.3591225682804466 1.2973504667815448 7 0.24224618938738582 1.020982640494318 8 0.1861795786221219 0.8967789256915657 9 0.13055808054724727 0.7074726083817221 >10 0.9606701115069196 12.552015897484303 >50 0.13841241905410126 5.703320452807771 >100 0.04348348226501588 4.257358514190701 >500 1.2920920523025627E-4 0.05476832740722103 >1k 1.9381380784538402E-4 0.20895984007925486 >5k 1.29209205230256E-4 0.4396247417304055 >10k+ 1.9381380784538402E-4 6.246584467328269 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 136352 1.3845116893313034 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13118 0.13319954485924693 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 11119 0.11290179442673934 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0153952192349971E-5 0.0 0.0 0.0 0.0 2 2.0307904384699942E-5 0.0 0.0 2.0307904384699942E-5 1.0153952192349971E-5 3 6.092371315409983E-5 0.0 0.0 2.0307904384699942E-5 1.0153952192349971E-5 4 7.107766534644981E-5 0.0 0.0 4.0615808769399884E-5 1.0153952192349971E-5 5 8.123161753879977E-5 0.0 0.0 5.076976096174986E-5 1.0153952192349971E-5 6 8.123161753879977E-5 0.0 0.0 5.076976096174986E-5 1.0153952192349971E-5 7 8.123161753879977E-5 0.0 0.0 7.107766534644981E-5 1.0153952192349971E-5 8 9.138556973114974E-5 0.0 0.0 7.107766534644981E-5 2.0307904384699942E-5 9 1.1169347411584969E-4 0.0 0.0 7.107766534644981E-5 2.0307904384699942E-5 10-11 1.1169347411584969E-4 0.0 0.0 8.123161753879977E-5 3.0461856577049913E-5 12-13 1.2692440240437465E-4 0.0 0.0 8.123161753879977E-5 4.0615808769399884E-5 14-15 1.3707835459672463E-4 0.0 0.0 9.138556973114974E-5 5.076976096174986E-5 16-17 1.4215533069289961E-4 0.0 0.0 1.0153952192349972E-4 5.076976096174986E-5 18-19 1.5738625898142455E-4 0.0 0.0 1.2692440240437465E-4 5.076976096174986E-5 20-21 1.6754021117377452E-4 0.0 0.0 1.6246323507759954E-4 5.076976096174986E-5 22-23 1.776941633661245E-4 0.0 0.0 1.776941633661245E-4 5.076976096174986E-5 24-25 1.9800206775082445E-4 0.0 0.0 1.9800206775082445E-4 6.092371315409983E-5 26-27 2.3861787652022434E-4 0.0 0.0 2.132329960393494E-4 7.107766534644981E-5 28-29 2.7415670919344926E-4 0.0 0.0 2.3861787652022434E-4 8.123161753879977E-5 30-31 2.893876374819742E-4 0.0 0.0 2.4369485261639933E-4 8.630859363497476E-5 32-33 3.0969554186667417E-4 0.0 0.0 2.7415670919344926E-4 9.138556973114974E-5 34-35 3.55388326732249E-4 0.0 0.0 3.0969554186667417E-4 1.0153952192349972E-4 36-37 3.6554227892459897E-4 0.0 0.0 3.198494940590241E-4 1.1169347411584969E-4 38-39 4.1123506379017386E-4 0.0 0.0 3.249264701551991E-4 1.2184742630819967E-4 40-41 4.5692784865574874E-4 0.0 0.0 3.3000344625137406E-4 1.2184742630819967E-4 42-43 4.823127291366237E-4 0.0 0.0 3.4015739844372404E-4 1.5230928288524956E-4 44-45 5.076976096174986E-4 0.0 0.0 3.50311350636074E-4 1.726171872699495E-4 46-47 5.330824900983736E-4 0.0 0.0 3.6554227892459897E-4 1.8277113946229949E-4 48-49 5.736982988677735E-4 0.0 0.0 3.6554227892459897E-4 2.0307904384699943E-4 50-51 5.990831793486484E-4 0.0 0.0 3.858501833092989E-4 2.3354090042404936E-4 52-53 6.396989881180482E-4 0.0 0.0 3.858501833092989E-4 2.4369485261639933E-4 54-55 7.056996773683231E-4 0.0 0.0 3.909271594054739E-4 2.538488048087493E-4 56-57 7.564694383300728E-4 0.0 0.0 4.0615808769399887E-4 2.640027570010993E-4 58-59 8.173931514841727E-4 0.0 0.0 4.1631203988634884E-4 2.7923368528962424E-4 60-61 9.037017451191475E-4 0.0 0.0 4.264659920786988E-4 2.8431066138579923E-4 62-63 9.646254582732474E-4 0.0 0.0 4.315429681748738E-4 2.8431066138579923E-4 64-65 0.0010204721953311722 0.0 0.0 4.5185087255957375E-4 2.8431066138579923E-4 66-67 0.001086472884581447 0.0 0.0 4.8738970523279866E-4 2.944646135781492E-4 68-69 0.0011778584543125966 0.0 0.0 5.280055140021986E-4 3.046185657704991E-4 70-71 0.0013250907611016714 0.0 0.0 5.483134183868985E-4 3.147725179628491E-4 72-73 0.001441861211313696 0.0 0.0 5.483134183868985E-4 3.249264701551991E-4 74-75 0.0015281698049486709 0.0 0.0 5.584673705792485E-4 3.3000344625137406E-4 76-77 0.0016906330400262703 0.0 0.0 5.787752749639484E-4 3.50311350636074E-4 78-79 0.0018886351077770948 0.0 0.0 5.940062032524735E-4 3.55388326732249E-4 80-81 0.002157714840874369 0.0 0.0 6.193910837333483E-4 3.55388326732249E-4 82-83 0.002497872239318093 0.0 0.0 6.396989881180483E-4 3.60465302828424E-4 84-85 0.0030715705381858666 0.0 0.0 6.75237820791273E-4 3.6554227892459897E-4 86-87 0.0037721932394580143 0.0 0.0 6.803147968874481E-4 3.858501833092989E-4 88-89 0.004929743789385911 0.0 0.0 6.853917729836231E-4 3.960041355016489E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 485 0.0 24.471842 3 CGCGTAG 240 0.0 23.739012 54-55 ACTCGCG 260 0.0 22.82922 50-51 CTCGCGT 275 0.0 20.719576 52-53 CGTACGC 305 0.0 20.235712 20-21 CAACGCA 7245 0.0 20.084272 12-13 ATCAACG 7470 0.0 19.383991 10-11 TCAACGC 6880 0.0 18.044598 10-11 GTATCAA 9630 0.0 17.156036 9 ACGCAGA 8605 0.0 16.992756 14-15 CGTAGCG 350 0.0 16.95562 56-57 AGTGGTA 10125 0.0 16.598629 5 TGGTATC 10180 0.0 16.182503 7 AACGCAG 7935 0.0 15.884794 12-13 GGTATCA 10315 0.0 15.878659 8 GTGGTAT 10660 0.0 15.5874405 6 AAGCAGT 11315 0.0 15.380724 1 ACGTACG 390 0.0 15.216699 20-21 CGCAGAG 8320 0.0 14.978557 14-15 AGCGACG 440 0.0 14.566567 58-59 >>END_MODULE