FastQCFastQC Report
Sat 18 Jun 2016
SRR578584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9024335
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115423512.790249918692071No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT295230.327148759437676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA215080.23833335087848576No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG124920.138425712254698No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA109840.12171533968984972No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT103380.11455691749031924No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC97540.10808552652356102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2569750.091.7769245
AAGCAGT2585950.091.619441
TGGTATC2578950.091.58347
GTATCAA2585750.091.517979
GTGGTAT2583350.091.490716
GGTATCA2588350.091.381018
GCAGTGG2599750.090.846793
AGCAGTG2615950.090.516642
CAGTGGT2607750.090.397484
TCAACGC2570800.046.3880912-13
CAACGCA2571550.046.3385612-13
ATCAACG2573700.046.3224910-11
AACGCAG2582550.046.19419514-15
ACGCAGA2584250.046.11972814-15
CGCAGAG2584550.046.11454416-17
TATCAAC2591100.046.0095910-11
AGAGTAC2522300.045.83165418-19
CAGAGTA2582200.045.65896218-19
GAGTACT1493050.045.588720-21
GCAGAGT2617100.045.3714416-17
Produced by FastQC (version 0.11.5)