##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578584_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9024335
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.076875248979565	31.0	31.0	34.0	28.0	34.0
2	31.427909868150948	33.0	31.0	34.0	28.0	34.0
3	31.548532274123247	34.0	31.0	34.0	28.0	34.0
4	34.93469812457095	37.0	35.0	37.0	32.0	37.0
5	34.966426002580796	37.0	35.0	37.0	32.0	37.0
6	35.004778856281376	37.0	35.0	37.0	32.0	37.0
7	34.94073757235298	37.0	35.0	37.0	32.0	37.0
8	34.97852938748395	37.0	35.0	37.0	32.0	37.0
9	36.48444666559918	39.0	37.0	39.0	32.0	39.0
10-11	36.37631088606529	39.0	37.0	39.0	32.0	39.0
12-13	36.440831374278545	39.0	37.0	39.0	32.0	39.0
14-15	37.7181334137086	40.0	38.0	41.0	32.0	41.0
16-17	37.677065456900706	40.0	38.0	41.0	32.0	41.0
18-19	37.60980437893762	40.0	38.0	41.0	32.0	41.0
20-21	37.53408389648656	40.0	38.0	41.0	32.0	41.0
22-23	37.269159334178084	39.5	37.5	41.0	31.5	41.0
24-25	37.22457344502393	40.0	37.0	41.0	31.0	41.0
26-27	37.13395385920403	40.0	37.0	41.0	31.0	41.0
28-29	36.982812029916886	40.0	37.0	41.0	30.0	41.0
30-31	36.746986786283976	40.0	36.5	41.0	30.0	41.0
32-33	36.49637895756308	40.0	36.0	41.0	30.0	41.0
34-35	36.33758819901965	39.5	36.0	41.0	29.0	41.0
36-37	36.043558334215206	39.0	35.5	40.5	28.0	41.0
38-39	35.122014142870356	38.0	35.0	40.0	27.0	41.0
40-41	34.885628747159764	38.0	34.5	40.0	26.0	41.0
42-43	34.575803314039206	37.5	34.0	40.0	24.5	41.0
44-45	34.28089704116702	37.0	34.0	40.0	24.0	41.0
46-47	34.117164145612946	37.0	33.5	40.0	23.5	41.0
48-49	34.10366858056577	37.0	33.5	40.0	23.0	41.0
50-51	33.171046786272896	37.0	32.5	39.5	19.0	40.5
52-53	32.704287019486756	37.5	33.0	39.5	10.0	40.5
54-55	32.25981493373196	38.0	32.0	40.0	2.0	41.0
56-57	31.718784375801654	38.0	31.0	40.0	2.0	41.0
58-59	31.425552132096158	37.0	31.0	40.0	2.0	41.0
60-61	31.165420665345422	37.0	31.0	40.0	2.0	41.0
62-63	30.83673694515995	36.5	30.0	40.0	2.0	41.0
64-65	30.546596840653635	36.0	30.0	40.0	2.0	41.0
66-67	30.211458018790303	35.5	29.0	39.0	2.0	41.0
68-69	29.875197396816496	35.0	29.0	39.0	2.0	41.0
70-71	29.57257443346241	35.0	29.0	38.5	2.0	40.5
72-73	29.246179635396956	35.0	28.5	37.5	2.0	40.0
74-75	28.880459557407832	35.0	28.0	37.0	2.0	39.5
76-77	28.50439517150017	35.0	27.5	36.5	2.0	39.0
78-79	28.17343948335251	35.0	27.0	36.0	2.0	39.0
80-81	27.84385813469912	35.0	26.0	36.0	2.0	37.5
82-83	27.56213964796298	34.0	26.0	35.0	2.0	37.0
84-85	27.274349411895727	34.0	26.0	35.0	2.0	37.0
86-87	27.003891754904934	34.0	25.0	35.0	2.0	36.0
88-89	26.761210161191933	34.0	25.0	35.0	2.0	36.0
90-91	26.515230208098437	34.0	24.5	35.0	2.0	36.0
92-93	26.22377964692135	34.0	23.5	35.0	2.0	35.0
94-95	25.93641237830821	34.0	20.0	35.0	2.0	35.0
96-97	25.669908530656276	34.0	18.5	35.0	2.0	35.0
98-99	25.37190690505173	34.0	8.5	35.0	2.0	35.0
100-101	24.54216083511971	33.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6101249825915893
1101	2	0.647584744349043
1101	3	0.5131781584768298
1101	4	0.5683308258154867
1101	5	0.5379734894957693
1101	6	0.5173587980980869
1101	7	0.46845967620401296
1101	8	0.4746362173275216
1101	9	0.6064846644961079
1101	10-11	0.6831165654316962
1101	12-13	0.6691213579878692
1101	14-15	0.8375986062947973
1101	16-17	0.7445331242778579
1101	18-19	0.7890945451187008
1101	20-21	0.8199460844779054
1101	22-23	0.8411455138683266
1101	24-25	0.7324972114533139
1101	26-27	0.8202976320755511
1101	28-29	0.9589572277515117
1101	30-31	1.0357840469879633
1101	32-33	1.0485408239621066
1101	34-35	1.1140380995008385
1101	36-37	1.0836823560219528
1101	38-39	1.041911260051556
1101	40-41	1.037593913057762
1101	42-43	1.0859285652701942
1101	44-45	1.2380614318044465
1101	46-47	1.1260955192718427
1101	48-49	1.0489254599576228
1101	50-51	1.0719582634267724
1101	52-53	0.932708997417393
1101	54-55	0.8637915357652233
1101	56-57	0.9013628385726307
1101	58-59	0.8419493645029874
1101	60-61	0.7335744856590978
1101	62-63	0.7144601166229805
1101	64-65	0.7186187382520615
1101	66-67	0.7119589725400886
1101	68-69	0.6365237368688952
1101	70-71	0.7716377198629125
1101	72-73	0.7049118076213006
1101	74-75	0.7223287189702461
1101	76-77	0.6978290580306918
1101	78-79	0.7237139116973559
1101	80-81	0.7387242208344205
1101	82-83	0.7904493921836142
1101	84-85	0.7451386222634824
1101	86-87	0.74622650839272
1101	88-89	0.7135003189501461
1101	90-91	0.7692920807611792
1101	92-93	0.7964648955753617
1101	94-95	0.8773187691923674
1101	96-97	0.8661402673242762
1101	98-99	0.8297577074492004
1101	100-101	0.9639989068468005
1102	1	0.4592049999406207
1102	2	0.4156479986936539
1102	3	0.4867875091035181
1102	4	0.45087667298743384
1102	5	0.4974961711091481
1102	6	0.5427687029454873
1102	7	0.5392390967438985
1102	8	0.4365937617189033
1102	9	0.5903385806497994
1102	10-11	0.6047958927190749
1102	12-13	0.5501681697215233
1102	14-15	0.69896460884231
1102	16-17	0.73574285790842
1102	18-19	0.8154856484003048
1102	20-21	0.8365572203982765
1102	22-23	0.8297926818976222
1102	24-25	0.885056299722315
1102	26-27	0.9206991508027116
1102	28-29	0.9765466478832678
1102	30-31	0.9803849538414724
1102	32-33	1.067307340139088
1102	34-35	0.9777116007897177
1102	36-37	1.0055376319796423
1102	38-39	1.0242466753372526
1102	40-41	1.0127581397107832
1102	42-43	1.0443566503158195
1102	44-45	1.1846748233201367
1102	46-47	1.2468222423741722
1102	48-49	1.1953101027427167
1102	50-51	1.1949136433728853
1102	52-53	0.9585450366362309
1102	54-55	0.897981737445491
1102	56-57	0.8657845984785126
1102	58-59	0.8845883948380333
1102	60-61	0.9103597155410199
1102	62-63	0.9574239393949746
1102	64-65	0.943214309906935
1102	66-67	0.9320068073698842
1102	68-69	0.8670483244761051
1102	70-71	0.8824369323833281
1102	72-73	0.8446227880261183
1102	74-75	0.7600389169950432
1102	76-77	0.8696001438669612
1102	78-79	0.8978004462485529
1102	80-81	0.8989486755925356
1102	82-83	0.8933878010905723
1102	84-85	0.9076145016423958
1102	86-87	0.900749674306514
1102	88-89	0.9401526938207212
1102	90-91	0.9183682879118784
1102	92-93	0.9086207446074077
1102	94-95	1.0042968785240625
1102	96-97	1.070495926604238
1102	98-99	1.1191069518866072
1102	100-101	1.2074973809649912
1103	1	0.43924199143982534
1103	2	0.48504834313929734
1103	3	0.3916330875576044
1103	4	0.4711391804734646
1103	5	0.4965167752972164
1103	6	0.5309052032455668
1103	7	0.4263117482151344
1103	8	0.4402574502255874
1103	9	0.5646421207285783
1103	10-11	0.5552834542793548
1103	12-13	0.6128913785512395
1103	14-15	0.6636665886188808
1103	16-17	0.7432907612904174
1103	18-19	0.744824879245293
1103	20-21	0.7818140774745075
1103	22-23	0.8707503646105152
1103	24-25	0.7571740454092932
1103	26-27	0.8234663295401035
1103	28-29	0.8584766020480643
1103	30-31	0.8553033380584409
1103	32-33	0.940944311493773
1103	34-35	0.9295179019188708
1103	36-37	1.0222233979830762
1103	38-39	0.8432947793625303
1103	40-41	0.98857472096104
1103	42-43	0.8639796879491826
1103	44-45	0.8586811739765849
1103	46-47	1.096788588514137
1103	48-49	1.1377982590154403
1103	50-51	1.0436833344014786
1103	52-53	0.9509195110903761
1103	54-55	0.8988421266132605
1103	56-57	0.8994810186239306
1103	58-59	0.831778819582695
1103	60-61	0.9114059796145142
1103	62-63	0.7249478377575223
1103	64-65	0.6729051701004316
1103	66-67	0.6927600969733554
1103	68-69	0.8467287210665475
1103	70-71	0.7731996334556399
1103	72-73	0.8475011128744825
1103	74-75	0.8321067697077993
1103	76-77	0.8016376546970463
1103	78-79	0.8116418042972029
1103	80-81	0.8809214512990167
1103	82-83	0.827071823374002
1103	84-85	0.8730871761451091
1103	86-87	0.9261188595408392
1103	88-89	0.9424087534561991
1103	90-91	0.8946711845207638
1103	92-93	0.9307478825168616
1103	94-95	0.8644443248692291
1103	96-97	0.8303620553565949
1103	98-99	0.8910862665430592
1103	100-101	0.9717343350621057
1104	1	0.34507626813639547
1104	2	0.3738885783246708
1104	3	0.3753843746778145
1104	4	0.374965377776185
1104	5	0.43662953717626607
1104	6	0.47204582602316947
1104	7	0.4378961341920089
1104	8	0.37177101338726715
1104	9	0.5065324425722011
1104	10-11	0.5009774789076076
1104	12-13	0.5242293855537312
1104	14-15	0.6259316962276174
1104	16-17	0.5842669406331495
1104	18-19	0.6636784940263212
1104	20-21	0.6737018835964292
1104	22-23	0.6456800880996454
1104	24-25	0.6884686435754332
1104	26-27	0.7324146404410072
1104	28-29	0.7562412320585068
1104	30-31	0.8269377600680698
1104	32-33	0.7969613680091712
1104	34-35	0.892481912011931
1104	36-37	0.8418612926996119
1104	38-39	0.91655480396917
1104	40-41	0.8772578366889832
1104	42-43	1.0420456788678507
1104	44-45	0.9806178861950485
1104	46-47	1.1660585027455284
1104	48-49	1.012674739412681
1104	50-51	0.9944298489604364
1104	52-53	0.9513010313686152
1104	54-55	0.9655105373062653
1104	56-57	0.9497242753038364
1104	58-59	0.952759199830723
1104	60-61	0.9202858784381789
1104	62-63	0.7986917767929178
1104	64-65	0.7363180160520209
1104	66-67	0.822186852260824
1104	68-69	0.8209836525615408
1104	70-71	0.7579702770130226
1104	72-73	0.7769110816492066
1104	74-75	0.8476994103625017
1104	76-77	0.8076005450412076
1104	78-79	0.7562782770671781
1104	80-81	0.8022157920605579
1104	82-83	0.7990321740446156
1104	84-85	0.8226095101935975
1104	86-87	0.857506691053274
1104	88-89	0.8400911881895539
1104	90-91	0.8015835463417353
1104	92-93	0.6926595152840171
1104	94-95	0.6633884496403155
1104	96-97	0.7911054865039375
1104	98-99	1.0555497400913936
1104	100-101	0.9821325306480766
1105	1	0.18656036292205513
1105	2	0.1314249001554586
1105	3	0.10420898382393773
1105	4	0.18319803172897764
1105	5	0.11865549891629001
1105	6	0.09722090712428155
1105	7	0.1100376266647487
1105	8	0.11007248355321764
1105	9	0.04651342508838496
1105	10-11	0.10921088890464858
1105	12-13	0.035780747993619855
1105	14-15	-0.05993593329112201
1105	16-17	-0.038444204547083416
1105	18-19	-0.1213569704933164
1105	20-21	-0.0050361564340448695
1105	22-23	-0.05681998222885909
1105	24-25	-0.1085707342620239
1105	26-27	-0.3717737484116981
1105	28-29	-0.13646674572657957
1105	30-31	-0.2915908315278273
1105	32-33	-0.28713236861086955
1105	34-35	-0.1886175205717322
1105	36-37	-0.14013845131076863
1105	38-39	-0.043085699653012455
1105	40-41	-0.05390818618243998
1105	42-43	0.10123225475102515
1105	44-45	0.04783605948272651
1105	46-47	0.03800805627680859
1105	48-49	0.08130772965559174
1105	50-51	-0.0032148170848813606
1105	52-53	-0.019992849444840033
1105	54-55	-0.10613008507017696
1105	56-57	0.03637041936443808
1105	58-59	0.02048354372005079
1105	60-61	-0.024793632932677667
1105	62-63	0.04073528695516515
1105	64-65	-0.045532658160173156
1105	66-67	-0.059724127237121394
1105	68-69	-0.03951991347791051
1105	70-71	-0.045020511110244854
1105	72-73	-0.04672946729465011
1105	74-75	-0.03713016630168653
1105	76-77	0.00811179896477654
1105	78-79	0.0060344937742122795
1105	80-81	0.10339637667208379
1105	82-83	0.0903162213654447
1105	84-85	0.05294326766980717
1105	86-87	0.11455483162800206
1105	88-89	0.23003831365091543
1105	90-91	0.19310502638871085
1105	92-93	0.17352945463062142
1105	94-95	0.19749958859598493
1105	96-97	0.21333380625377885
1105	98-99	0.20034978349530164
1105	100-101	0.20101666383422412
1106	1	-0.16980600527260492
1106	2	-0.12256055650262354
1106	3	-0.1640840118980087
1106	4	-0.17089425779746392
1106	5	-0.21529917080005134
1106	6	-0.22238415115207033
1106	7	-0.26469713783733795
1106	8	-0.25259573393967116
1106	9	-0.23720267103014692
1106	10-11	-0.38485171108565197
1106	12-13	-0.4207201351445278
1106	14-15	-0.3966885428235756
1106	16-17	-0.43378400067790324
1106	18-19	-0.43801146847266637
1106	20-21	-0.23985366277750586
1106	22-23	-0.2324763464010715
1106	24-25	-0.3415456881013412
1106	26-27	-0.40074792868523446
1106	28-29	-0.4385469441539982
1106	30-31	-0.42602542772599605
1106	32-33	-0.3395582172359255
1106	34-35	-0.3245995846799943
1106	36-37	-0.4255438187347309
1106	38-39	-0.45886056675916365
1106	40-41	-0.4247439145845604
1106	42-43	-0.5073267956788641
1106	44-45	-0.4579671133628054
1106	46-47	-0.5087187539727296
1106	48-49	-0.681631087696644
1106	50-51	-0.6847834793281748
1106	52-53	-0.7642993691078459
1106	54-55	-0.662113757752735
1106	56-57	-0.6540733839247572
1106	58-59	-0.5964455643422113
1106	60-61	-0.7732169706377476
1106	62-63	-0.6541823609335466
1106	64-65	-0.6337262212136991
1106	66-67	-0.7916640912805093
1106	68-69	-0.9388722850633151
1106	70-71	-0.7883155985714296
1106	72-73	-0.7758747884371822
1106	74-75	-0.8507209250209797
1106	76-77	-0.9070247960252438
1106	78-79	-0.8487004526321726
1106	80-81	-0.8356040784944305
1106	82-83	-0.9370396940808483
1106	84-85	-0.971282895463343
1106	86-87	-0.9603315098212271
1106	88-89	-1.0384411913703566
1106	90-91	-0.9358770419214366
1106	92-93	-0.9414119645777248
1106	94-95	-0.9352715109662633
1106	96-97	-0.8723099465635151
1106	98-99	-0.9563303239607528
1106	100-101	-1.0789949956064824
1107	1	-0.42446539431693964
1107	2	-0.47283224381143185
1107	3	-0.4254869795041216
1107	4	-0.30374818431751294
1107	5	-0.3867226837635087
1107	6	-0.39568369698938
1107	7	-0.30516099158325716
1107	8	-0.29851823153479273
1107	9	-0.3909577361019032
1107	10-11	-0.40213078751525444
1107	12-13	-0.42539465042016644
1107	14-15	-0.6896822840596073
1107	16-17	-0.521551843289771
1107	18-19	-0.49426610783988423
1107	20-21	-0.4119625394301991
1107	22-23	-0.442883640879586
1107	24-25	-0.46131599113162025
1107	26-27	-0.3401143280605936
1107	28-29	-0.4092046118544417
1107	30-31	-0.4424942138922745
1107	32-33	-0.35848020186244867
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2108	24-25	-1.2394844544274193
2108	26-27	-1.3404395646062142
2108	28-29	-1.5919997090447637
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2108	36-37	-2.297684523936219
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2208	20-21	-1.2446193216361152
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2308	42-43	-2.700269139740456
2308	44-45	-2.7742323244612344
2308	46-47	-2.855650587531727
2308	48-49	-2.5837896529498536
2308	50-51	-2.519472950738738
2308	52-53	-2.4455936017037203
2308	54-55	-2.082182530412041
2308	56-57	-2.0018850437870483
2308	58-59	-1.923161324360116
2308	60-61	-1.9423868967868358
2308	62-63	-2.0352978678204074
2308	64-65	-2.003873006892892
2308	66-67	-2.151923324461972
2308	68-69	-1.9577984883198774
2308	70-71	-1.9095916618938205
2308	72-73	-1.905318011090234
2308	74-75	-1.7513050675233615
2308	76-77	-1.6870833789438642
2308	78-79	-1.758083516344037
2308	80-81	-2.3623922552067853
2308	82-83	-2.1702911414937773
2308	84-85	-1.9070688325311274
2308	86-87	-1.7986525390004608
2308	88-89	-1.7437060990068254
2308	90-91	-1.970401260168181
2308	92-93	-1.9767622661547009
2308	94-95	-2.037323150149202
2308	96-97	-2.0278030154147046
2308	98-99	-2.0638055470450034
2308	100-101	-2.2358205446774235
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81813.0
3	13773.0
4	12224.0
5	11644.0
6	13770.0
7	16607.0
8	20253.0
9	23971.0
10	27343.0
11	33324.0
12	32944.0
13	36890.0
14	41504.0
15	48544.0
16	62076.0
17	86506.0
18	134763.0
19	220962.0
20	317282.0
21	223327.0
22	116906.0
23	96855.0
24	97499.0
25	102550.0
26	109650.0
27	122055.0
28	137877.0
29	160128.0
30	187639.0
31	224183.0
32	273880.0
33	344573.0
34	454925.0
35	663676.0
36	1099113.0
37	1509673.0
38	1526586.0
39	336551.0
40	496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.064568187633792	48.75230774893643	24.36869789964358	16.8144261637862
2	15.736278206580357	50.38151894162739	18.677624313926966	15.204578537865288
3	44.15497261190082	20.80907738870916	19.524215648979837	15.511734350410183
4	15.785208560577551	19.322202237912528	20.42786478504203	44.46472441646789
5	15.77532367168618	47.85054007389084	21.492126029662806	14.882010224760176
6	44.36937111642077	20.378650151691883	19.36072216372528	15.891256568162056
7	16.593035236484546	19.775435588603386	48.817881998965895	14.813647175946171
8	43.96465286741656	20.551089377154284	20.183353664590985	15.300904090838168
9	43.53335886681012	20.183382228168213	20.81767413077573	15.465584774245942
10-11	15.451278604982496	34.21045194797365	34.52734371017312	15.810925736870733
12-13	15.344123546488753	20.48397139426471	34.43086330350004	29.741041755746494
14-15	15.515172303159583	48.507461404640836	20.20270927355307	15.774657018646515
16-17	29.579321223252236	20.31981376277615	20.3250718527132	29.775793161258417
18-19	15.66676407057007	34.30118904790067	20.313467701962672	29.71857917956659
20-21	29.751966018965625	34.30672067770587	20.335602828265994	15.605710475062521
22-23	29.618007917957488	20.226528448697998	34.46510570297055	15.690357930373967
24-25	15.628264292827259	34.082185505496824	20.759479826341355	29.530070375334567
26-27	15.82864963099222	25.65570355266949	42.62744065955985	15.88820615677844
28-29	26.829873576451025	20.140668596369157	37.24044343486157	15.789014392318245
30-31	24.363863236289028	21.352693435255436	37.67776295908026	16.605680369375275
32-33	18.618120315715615	23.30565702206614	39.447342537790085	18.628880124428157
34-35	18.1253859114025	22.665188918729996	40.376433440616516	18.832991729250985
36-37	18.099336868453996	22.760727338166724	40.14767123843249	18.992264554946793
38-39	18.69595436036403	23.09390668484321	39.562798936091696	18.647340018701065
40-41	18.759843174106443	23.226139791356847	39.364678934665484	18.649338099871233
42-43	19.138789595884628	23.10680122680997	39.30868244254973	18.44572673475567
44-45	18.757712533487467	22.99248626371191	39.48100744303807	18.768793759762563
46-47	18.969142445425643	23.025779112653947	39.236041833844006	18.769036608076405
48-49	19.06309768702411	23.258425304078443	38.985864590199775	18.692612418697667
50-51	19.184948653078322	23.687647594003042	38.22858384004273	18.898819912875904
52-53	19.416917381603454	24.29379054520866	37.03305342803774	19.256238645150148
54-55	20.266038079329373	25.122189302049115	35.22089674122467	19.39087587739684
56-57	20.569786016245587	25.979305846238237	33.76469453899221	19.686213598523963
58-59	20.80396401969695	26.754603380575716	32.89594265234248	19.545489947384866
60-61	20.804228046450426	26.898290920137796	32.482645541965	19.81483549144678
62-63	20.304620392682708	26.89670256110127	32.72745839657473	20.07121864964129
64-65	20.45526959479129	26.533710886744625	32.93241026228158	20.078609256182503
66-67	20.231355702499844	26.16847538763339	33.147313169255696	20.452855740611074
68-69	20.332866838617242	26.24778459395652	33.28678372976507	20.13256483766117
70-71	19.954944404730586	26.10835222160842	33.8165713922433	20.12013198141769
72-73	19.965751509791403	25.83552182269287	33.8011830267895	20.397543640726226
74-75	20.347892297606464	25.762773241996662	33.28585028967449	20.603484170722393
76-77	20.386587685849864	26.09271313752349	33.24051589408493	20.28018328254172
78-79	20.56958248296267	26.4847109731122	32.26390038021771	20.68180616370741
80-81	20.62645002332465	26.5440542996146	32.24153909864309	20.58795657841766
82-83	20.999838026317395	26.745765664019988	31.816662432423136	20.43773387723948
84-85	20.99452034474359	26.901324544494194	31.77932664573415	20.32482846502807
86-87	21.030503048218975	26.885693008823125	31.637836880675884	20.445967062282016
88-89	21.011120908790755	26.85055215126868	31.395365736976828	20.74296120296374
90-91	21.27422634153616	26.98145265733031	31.317422993654148	20.426898007479384
92-93	21.19803522263439	27.145885268725305	31.20064259270799	20.455436915932317
94-95	21.00282338990805	27.343164458605358	30.844205680053623	20.809806471432974
96-97	20.851969249649017	27.152216301730398	31.321441455859784	20.6743729927608
98-99	20.903262132244237	27.11176821213328	31.078267526658447	20.90670212896403
100-101	21.11136439779556	27.027981693900593	30.736662777862318	21.123991130441528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9129.0
1	5501.0
2	1367.0
3	746.0
4	602.5
5	559.5
6	689.0
7	879.0
8	832.5
9	923.5
10	1566.5
11	9127.5
12	155219.0
13	171813.5
14	43610.0
15	39135.0
16	42947.0
17	48003.0
18	52689.0
19	57311.5
20	61843.0
21	66161.0
22	71372.0
23	77396.5
24	84226.0
25	92253.0
26	103018.0
27	115337.0
28	126293.0
29	137301.5
30	148057.0
31	158832.5
32	172020.5
33	186842.0
34	208360.5
35	220140.0
36	225300.5
37	237202.5
38	244387.0
39	252274.5
40	263186.0
41	269178.0
42	270480.0
43	269853.5
44	274709.5
45	279360.5
46	273442.5
47	270431.0
48	269753.5
49	266883.0
50	262872.5
51	254966.5
52	250256.5
53	244478.0
54	239335.0
55	233997.0
56	221655.5
57	206338.0
58	200075.0
59	168612.0
60	114959.5
61	83295.5
62	61953.0
63	45393.0
64	32658.5
65	22923.0
66	14789.0
67	8996.5
68	5454.0
69	3463.5
70	2367.5
71	1844.5
72	1500.5
73	1221.5
74	1077.5
75	946.5
76	835.5
77	726.5
78	601.0
79	500.5
80	413.0
81	364.0
82	313.5
83	257.0
84	211.0
85	178.5
86	138.5
87	95.5
88	73.0
89	62.0
90	45.0
91	24.0
92	23.0
93	22.0
94	14.0
95	10.5
96	7.0
97	4.0
98	2.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0523362663287655
2	0.09453328139968208
3	0.08471538346038794
4	0.055815747088289604
5	0.08633323120207749
6	0.001340819018797507
7	0.0012078452318093245
8	0.005130571947960709
9	0.009828979088209823
10-11	0.01005060206652346
12-13	0.016943076692077588
14-15	0.011829126467490401
16-17	0.001612307167231713
18-19	6.20544339278185E-4
20-21	7.756804240977312E-5
22-23	4.2108365879591126E-4
24-25	8.809513387967092E-4
26-27	0.0014959551036170533
28-29	0.0016178477416895537
30-31	0.0049865170120568444
32-33	1.2189263807250063E-4
34-35	0.002692719186510696
36-37	3.5459676530182E-4
38-39	0.0027592060800047872
40-41	0.003490561908439791
42-43	0.0013020349975926204
44-45	6.981123816879582E-4
46-47	0.001224466955182847
48-49	0.002487717931570581
50-51	0.0047094882891647975
52-53	0.13232553977661513
54-55	0.14189965243976427
56-57	0.1279651076782943
58-59	0.12143831096695767
60-61	0.10930999347874386
62-63	0.11373137189610093
64-65	0.1164739562527322
66-67	0.12459643840792702
68-69	0.13788273595782957
70-71	0.12464076300358973
72-73	0.11642963165706947
74-75	0.12124993143539109
76-77	0.13319540996649615
78-79	0.14752887608893067
80-81	0.13752813919252774
82-83	0.11662909233755175
84-85	0.12284007630479143
86-87	0.12414211130238405
88-89	0.12037452067105221
90-91	0.13110107282143227
92-93	0.1478169859607384
94-95	0.15783988515497263
96-97	0.15836623972846753
98-99	0.1407139695057863
100-101	0.1297657943770926
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9024335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.60922016778793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.49115825271063	35.44674990301967
2	5.679256098383079	4.499018103802154
3	1.6890529454751477	2.0070620997712756
4	0.8392214165511243	1.329636234307854
5	0.5009066920639546	0.9920261724739763
6	0.3271910540087848	0.7775869497098731
7	0.22536351714070274	0.6248531217749203
8	0.16158056281013997	0.5120064069745536
9	0.10867852738462236	0.38742045468196296
>10	0.8748994878637204	7.0367301219578735
>50	0.06014830858483083	1.5506704683558357
>100	0.03137583174084895	2.2877495533875596
>500	0.004867799738540577	1.3961694364161046
>1k	0.005368896770449125	4.599448269483435
>5k	5.010970319085851E-4	1.4195334416071383
>10k+	4.2951174163593006E-4	35.13333926227574
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1154235	12.790249918692071	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	29523	0.327148759437676	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA	21508	0.23833335087848576	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	12492	0.138425712254698	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA	10984	0.12171533968984972	No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT	10338	0.11455691749031924	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC	9754	0.10808552652356102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.216229783136375E-5	0.0	0.0	0.0	0.0
2	4.43245956627275E-5	0.0	0.0	0.0	0.0
3	4.43245956627275E-5	0.0	0.0	2.216229783136375E-5	0.0
4	4.43245956627275E-5	0.0	0.0	2.216229783136375E-5	0.0
5	4.43245956627275E-5	0.0	0.0	2.216229783136375E-5	0.0
6	4.43245956627275E-5	0.0	0.0	3.324344674704563E-5	0.0
7	5.540574457840938E-5	0.0	0.0	3.324344674704563E-5	0.0
8	5.540574457840938E-5	0.0	0.0	3.324344674704563E-5	0.0
9	7.756804240977314E-5	0.0	0.0	3.324344674704563E-5	0.0
10-11	9.418976578329595E-5	0.0	0.0	5.540574457840938E-5	5.540574457840938E-6
12-13	1.2189263807250063E-4	0.0	0.0	5.540574457840938E-5	1.1081148915681875E-5
14-15	1.4959551036170532E-4	0.0	0.0	5.540574457840938E-5	1.1081148915681875E-5
16-17	1.6621723373522814E-4	0.0	0.0	5.540574457840938E-5	1.1081148915681875E-5
18-19	1.9392010602443282E-4	0.0	0.0	7.756804240977314E-5	1.1081148915681875E-5
20-21	2.1054182939795563E-4	0.0	0.0	8.8649191325455E-5	1.1081148915681875E-5
22-23	2.1608240385579658E-4	0.0	0.0	8.8649191325455E-5	1.1081148915681875E-5
24-25	2.4378527614500126E-4	0.0	0.0	9.973034024113688E-5	1.1081148915681875E-5
26-27	2.770287228920469E-4	0.0	0.0	1.0527091469897783E-4	1.1081148915681875E-5
28-29	2.881098718077288E-4	0.0	0.0	1.2189263807250064E-4	1.1081148915681875E-5
30-31	3.213533185547744E-4	0.0	0.0	1.329737869881825E-4	1.1081148915681875E-5
32-33	3.490561908439791E-4	0.0	0.0	1.3851436144602346E-4	1.1081148915681875E-5
34-35	3.6013733975966096E-4	0.0	0.0	1.4959551036170532E-4	1.1081148915681875E-5
36-37	3.7675906313318374E-4	0.0	0.0	1.7175780819306908E-4	1.1081148915681875E-5
38-39	3.9892136096454753E-4	5.540574457840938E-6	0.0	1.883795315665919E-4	1.1081148915681875E-5
40-41	4.2108365879591126E-4	1.1081148915681875E-5	0.0	1.9946068048227376E-4	1.1081148915681875E-5
42-43	4.5986768000079784E-4	1.1081148915681875E-5	0.0	2.050012549401147E-4	1.1081148915681875E-5
44-45	5.208139990370481E-4	1.1081148915681875E-5	0.0	2.1054182939795563E-4	1.1081148915681875E-5
46-47	5.485168713262529E-4	1.1081148915681875E-5	0.0	2.2162297831363753E-4	1.6621723373522814E-5
48-49	5.595980202419348E-4	1.1081148915681875E-5	0.0	2.2162297831363753E-4	2.216229783136375E-5
50-51	5.873008925311394E-4	1.1081148915681875E-5	0.0	2.4378527614500126E-4	2.216229783136375E-5
52-53	6.260849137360259E-4	1.1081148915681875E-5	0.0	2.493258506028422E-4	2.216229783136375E-5
54-55	6.593283604830716E-4	1.1081148915681875E-5	0.0	2.936504462655697E-4	2.216229783136375E-5
56-57	7.258152539771629E-4	1.1081148915681875E-5	0.0	3.047315951812516E-4	2.7702872289204687E-5
58-59	8.03383296386936E-4	1.1081148915681875E-5	0.0	3.4351561638613817E-4	3.324344674704563E-5
60-61	8.80951338796709E-4	1.1081148915681875E-5	0.0	3.8784021204886564E-4	3.324344674704563E-5
62-63	9.474382322908004E-4	1.1081148915681875E-5	0.0	3.8784021204886564E-4	3.324344674704563E-5
64-65	0.0010305468491584144	1.1081148915681875E-5	0.0	4.044619354223885E-4	3.324344674704563E-5
66-67	0.0011081148915681877	1.1081148915681875E-5	0.0	4.266242332537522E-4	3.324344674704563E-5
68-69	0.00124108867855637	1.1081148915681875E-5	0.0	4.543271055429569E-4	4.432459566272751E-5
70-71	0.001340819018797507	1.1081148915681875E-5	0.0	4.654082544586388E-4	5.540574457840938E-5
72-73	0.0014571710824121667	1.1081148915681875E-5	0.0	4.7094882891647973E-4	5.540574457840938E-5
74-75	0.0016233883161473946	1.1081148915681875E-5	0.0	4.986517012056844E-4	5.540574457840938E-5
76-77	0.0017729838265091	1.1081148915681875E-5	0.0	4.986517012056844E-4	5.540574457840938E-5
78-79	0.002055553123858988	1.1081148915681875E-5	0.0	4.986517012056844E-4	5.540574457840938E-5
80-81	0.0023381224212088757	1.1081148915681875E-5	0.0	5.152734245792072E-4	5.540574457840938E-5
82-83	0.0026539351653058095	1.1081148915681875E-5	0.0	5.3189514795273E-4	5.540574457840938E-5
84-85	0.0031802897388006983	1.1081148915681875E-5	0.0	5.485168713262529E-4	5.540574457840938E-5
86-87	0.00390056441832002	1.6621723373522814E-5	0.0	5.651385946997756E-4	5.540574457840938E-5
88-89	0.005202599415912641	2.216229783136375E-5	0.0	5.651385946997756E-4	5.540574457840938E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGTA	256975	0.0	91.776924	5
AAGCAGT	258595	0.0	91.61944	1
TGGTATC	257895	0.0	91.5834	7
GTATCAA	258575	0.0	91.51797	9
GTGGTAT	258335	0.0	91.49071	6
GGTATCA	258835	0.0	91.38101	8
GCAGTGG	259975	0.0	90.84679	3
AGCAGTG	261595	0.0	90.51664	2
CAGTGGT	260775	0.0	90.39748	4
TCAACGC	257080	0.0	46.38809	12-13
CAACGCA	257155	0.0	46.33856	12-13
ATCAACG	257370	0.0	46.32249	10-11
AACGCAG	258255	0.0	46.194195	14-15
ACGCAGA	258425	0.0	46.119728	14-15
CGCAGAG	258455	0.0	46.114544	16-17
TATCAAC	259110	0.0	46.00959	10-11
AGAGTAC	252230	0.0	45.831654	18-19
CAGAGTA	258220	0.0	45.658962	18-19
GAGTACT	149305	0.0	45.5887	20-21
GCAGAGT	261710	0.0	45.37144	16-17
>>END_MODULE