FastQCFastQC Report
Sat 18 Jun 2016
SRR578584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9024335
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000471.1086357055672247No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122760.1360321840889107No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107410.11902262050333902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4800.024.6980923
CGCGTAG2450.023.21952854-55
ACTCGCG2700.021.06951550-51
CAACGCA69050.020.2876112-13
ATCAACG70900.019.79167410-11
CGTACGC3250.018.2357620-21
CGTAGCG3250.018.23333556-57
CTCGCGT3250.018.23323452-53
TCAACGC66600.017.58165610-11
ACGCAGA80850.017.414614-15
GTATCAA90550.016.440939
TCGCGTA3900.016.4099152-53
AGTGGTA95600.015.7230615
AACGCAG75400.015.59255912-13
ACGTACG4000.015.40921720-21
TGGTATC96650.015.4050647
CGCTAGT3650.015.31401390-91
GGTATCA96950.015.1617598
AAGCAGT104650.014.7724531
GTGGTAT102250.014.60773856
Produced by FastQC (version 0.11.5)