##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578584_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9024335 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01754123711055 31.0 31.0 34.0 30.0 34.0 2 32.290171962809445 34.0 31.0 34.0 30.0 34.0 3 32.35939246492955 34.0 31.0 34.0 30.0 34.0 4 35.92252404193771 37.0 35.0 37.0 35.0 37.0 5 35.802318841222096 37.0 35.0 37.0 33.0 37.0 6 35.77709094354321 37.0 35.0 37.0 33.0 37.0 7 35.770373329447544 37.0 35.0 37.0 33.0 37.0 8 35.68831786497287 37.0 35.0 37.0 33.0 37.0 9 37.332043856971175 39.0 37.0 39.0 34.0 39.0 10-11 37.32811503562313 39.0 37.0 39.0 34.0 39.0 12-13 37.323911401781956 39.0 37.0 39.0 34.0 39.0 14-15 38.6440219140801 40.0 38.0 41.0 34.0 41.0 16-17 38.60277826565614 40.0 38.0 41.0 34.0 41.0 18-19 38.55391316922521 40.0 38.0 41.0 34.0 41.0 20-21 38.492815204666044 40.0 38.0 41.0 34.0 41.0 22-23 38.3744921924995 40.0 38.0 41.0 34.0 41.0 24-25 38.29427608793335 40.0 38.0 41.0 33.0 41.0 26-27 38.13090632162924 40.0 38.0 41.0 33.0 41.0 28-29 38.03545469001317 40.0 37.0 41.0 33.0 41.0 30-31 37.94066155567141 40.0 37.0 41.0 33.0 41.0 32-33 37.812011632990135 40.0 37.0 41.0 33.0 41.0 34-35 37.68321355534785 40.0 37.0 41.0 32.0 41.0 36-37 37.55906546022505 40.0 37.0 41.0 32.0 41.0 38-39 37.43363893295185 40.0 36.5 41.0 31.0 41.0 40-41 37.290872291420925 40.0 36.0 41.0 31.0 41.0 42-43 37.13567298864681 39.0 36.0 41.0 31.0 41.0 44-45 36.98367868657358 39.0 36.0 41.0 31.0 41.0 46-47 37.06039237240196 39.0 36.0 41.0 31.0 41.0 48-49 37.04210049826386 39.0 36.0 41.0 31.0 41.0 50-51 37.02161511069791 39.0 36.0 41.0 31.0 41.0 52-53 37.03810729544061 39.0 36.0 41.0 31.0 41.0 54-55 36.92120217168356 39.0 36.0 41.0 31.0 41.0 56-57 36.783212447232955 39.0 35.0 41.0 31.0 41.0 58-59 36.579787541131836 39.0 35.0 41.0 30.0 41.0 60-61 36.36246266345387 39.0 35.0 41.0 30.0 41.0 62-63 36.10409453993009 38.0 35.0 40.0 29.5 41.0 64-65 35.80559376397264 38.0 35.0 40.0 29.0 41.0 66-67 35.501221641262205 37.0 34.0 40.0 29.0 41.0 68-69 35.17592515127153 37.0 34.0 39.5 29.0 41.0 70-71 34.81787705132844 36.0 34.0 39.0 28.0 41.0 72-73 34.40355815691683 36.0 34.0 39.0 28.0 41.0 74-75 34.018736006586636 35.0 33.5 38.5 28.0 40.0 76-77 32.57917652658063 34.5 31.5 36.5 26.0 39.0 78-79 33.175114842257074 35.0 33.0 37.0 26.0 39.0 80-81 33.11059922974934 35.0 33.0 36.5 27.0 39.0 82-83 32.828211607835925 35.0 33.0 36.0 26.0 38.0 84-85 32.53187885866382 35.0 33.0 36.0 26.0 37.0 86-87 32.2410505594041 35.0 33.0 35.0 26.0 37.0 88-89 31.982953480782793 35.0 32.5 35.0 25.5 36.5 90-91 31.737948391765155 35.0 32.0 35.0 25.0 36.0 92-93 31.49633446675018 35.0 32.0 35.0 25.0 36.0 94-95 31.24284897446737 35.0 32.0 35.0 24.0 36.0 96-97 30.644082417153175 35.0 31.5 35.0 20.5 35.0 98-99 30.626233733565964 34.0 32.0 35.0 19.5 35.0 100-101 30.054354808415248 34.0 31.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40571460827838024 1101 2 0.39062922536015776 1101 3 0.3806491559141918 1101 4 0.2686989963973474 1101 5 0.26839079998678983 1101 6 0.2685713852263305 1101 7 0.3106526227888011 1101 8 0.3492152221535676 1101 9 0.43121373473739055 1101 10-11 0.4329873830304436 1101 12-13 0.43306474937588746 1101 14-15 0.5841369652247081 1101 16-17 0.5597477850314334 1101 18-19 0.564617815179048 1101 20-21 0.6125925739484828 1101 22-23 0.6285244205785077 1101 24-25 0.6595826163943457 1101 26-27 0.6742072039425651 1101 28-29 0.6726448422713744 1101 30-31 0.6217419469321968 1101 32-33 0.6025418453287585 1101 34-35 0.6164325340626888 1101 36-37 0.6481124998140757 1101 38-39 0.6499151118904862 1101 40-41 0.6615483174073162 1101 42-43 0.6745801150894692 1101 44-45 0.6518392826509327 1101 46-47 0.6883165365755346 1101 48-49 0.6795629637851377 1101 50-51 0.6344097866591056 1101 52-53 0.6135206495240553 1101 54-55 0.7132003238212477 1101 56-57 0.6884054169352041 1101 58-59 0.7311707519769826 1101 60-61 0.6695371798283674 1101 62-63 0.6794550987595827 1101 64-65 0.6981853283226513 1101 66-67 0.6831154258376912 1101 68-69 0.7263421316009016 1101 70-71 0.6863195984633705 1101 72-73 0.6413277256839152 1101 74-75 0.6740535657513718 1101 76-77 0.6564342536847292 1101 78-79 0.5805994793558327 1101 80-81 0.5135060935449118 1101 82-83 0.5145695897076479 1101 84-85 0.43741143925959847 1101 86-87 0.3936418892034581 1101 88-89 0.4475386384316593 1101 90-91 0.42367308863061126 1101 92-93 0.48272561966323124 1101 94-95 0.4822139678019788 1101 96-97 0.8851511407150277 1101 98-99 0.6547704096460869 1101 100-101 0.6103255281004216 1102 1 0.4052318866726523 1102 2 0.3723047040314569 1102 3 0.33279729468888064 1102 4 0.24821637136225405 1102 5 0.29935916057869605 1102 6 0.263299115373556 1102 7 0.25987332276486086 1102 8 0.2805040314773777 1102 9 0.4184504649651615 1102 10-11 0.36054964799969014 1102 12-13 0.36295604412786275 1102 14-15 0.4597021534160106 1102 16-17 0.4855512836302722 1102 18-19 0.49482961523699487 1102 20-21 0.5335788358941969 1102 22-23 0.5394749016568312 1102 24-25 0.6157033836040995 1102 26-27 0.6365931719819997 1102 28-29 0.6853212445210701 1102 30-31 0.6880510647710238 1102 32-33 0.7064714222064978 1102 34-35 0.7337117282667833 1102 36-37 0.7591898791796581 1102 38-39 0.7691441908821375 1102 40-41 0.7380859988611022 1102 42-43 0.7721086870379281 1102 44-45 0.7660708674795131 1102 46-47 0.8137954723229299 1102 48-49 0.8015619161910976 1102 50-51 0.7769991311394762 1102 52-53 0.6987062346017225 1102 54-55 0.7369828161230245 1102 56-57 0.6714361967067717 1102 58-59 0.7090169385591878 1102 60-61 0.7684722491280667 1102 62-63 0.6922782153539444 1102 64-65 0.6611905456893368 1102 66-67 0.6779001938383189 1102 68-69 0.7298889447160093 1102 70-71 0.6788613851953542 1102 72-73 0.6719986513523892 1102 74-75 0.6118120451464719 1102 76-77 0.6216542409326777 1102 78-79 0.656777718065868 1102 80-81 0.6366323600970674 1102 82-83 0.6224917044908764 1102 84-85 0.6025549048049825 1102 86-87 0.6161801182153752 1102 88-89 0.5295501710705182 1102 90-91 0.5262567122876369 1102 92-93 0.5582653508353168 1102 94-95 0.6357988230372023 1102 96-97 1.0267561686085465 1102 98-99 0.7783210376959708 1102 100-101 0.8028673457508013 1103 1 0.33476958595665707 1103 2 0.31016984352103805 1103 3 0.27109862079498726 1103 4 0.21349522060970827 1103 5 0.21122452717529683 1103 6 0.24469410419944637 1103 7 0.2876732042714565 1103 8 0.08405448403943439 1103 9 0.1738252123968138 1103 10-11 0.3649724914373067 1103 12-13 0.3684164587008141 1103 14-15 0.4781301638590847 1103 16-17 0.46627727227556903 1103 18-19 0.39946581417120086 1103 20-21 0.4053316241300564 1103 22-23 0.43725043331340174 1103 24-25 0.2829624633138508 1103 26-27 0.2993213175024323 1103 28-29 0.4752142059035691 1103 30-31 0.31601929897866654 1103 32-33 0.5304375816567912 1103 34-35 0.5205526247236278 1103 36-37 0.5579666191867148 1103 38-39 0.5985900401324784 1103 40-41 0.49593880143549285 1103 42-43 0.5644729737534817 1103 44-45 0.6694323192065994 1103 46-47 0.53565920297914 1103 48-49 0.559520746949218 1103 50-51 0.526400399410285 1103 52-53 0.5862272219526972 1103 54-55 0.6536136963263246 1103 56-57 0.587442473264737 1103 58-59 0.33181329156689543 1103 60-61 0.34302520596201447 1103 62-63 0.21795993737688235 1103 64-65 0.4632834331016795 1103 66-67 0.4524554169308672 1103 68-69 0.4183586668274728 1103 70-71 0.3883830683021543 1103 72-73 0.5938154690120783 1103 74-75 0.515640541816687 1103 76-77 0.367984133765745 1103 78-79 0.4344527259969624 1103 80-81 0.4419158781900805 1103 82-83 0.5113808608684991 1103 84-85 0.5200154233082657 1103 86-87 0.35067773695978843 1103 88-89 0.33709941688595535 1103 90-91 0.43259969905929196 1103 92-93 0.38273350982177945 1103 94-95 0.45468371634662574 1103 96-97 0.6033768129573502 1103 98-99 0.37600191897385216 1103 100-101 0.6029885063831486 1104 1 0.3241698604541696 1104 2 0.3111566130023036 1104 3 0.26011998016657145 1104 4 0.18845371897814545 1104 5 0.24020584652240728 1104 6 0.24024392237147651 1104 7 0.23583219135090872 1104 8 0.30645877521520504 1104 9 0.27660208856723756 1104 10-11 0.3475853845460364 1104 12-13 0.33364745677821617 1104 14-15 0.3915493111686317 1104 16-17 0.41337037411999233 1104 18-19 0.43143034863864216 1104 20-21 0.3841726641908423 1104 22-23 0.5142689943625811 1104 24-25 0.47225233690019763 1104 26-27 0.5358116314295032 1104 28-29 0.5235611994372746 1104 30-31 0.5376004942272132 1104 32-33 0.5372121456962233 1104 34-35 0.5467218851239934 1104 36-37 0.599290881094575 1104 38-39 0.5993236945709199 1104 40-41 0.5413995335081765 1104 42-43 0.674032038116529 1104 44-45 0.5716638102410272 1104 46-47 0.4651835931538173 1104 48-49 0.5732108425412008 1104 50-51 0.4739838313570601 1104 52-53 0.5953521264077679 1104 54-55 0.6670117286820059 1104 56-57 0.6604009565466171 1104 58-59 0.6371989470383781 1104 60-61 0.536274884614528 1104 62-63 0.38114646791513707 1104 64-65 0.5617186993050822 1104 66-67 0.5694890054317412 1104 68-69 0.6229560980850977 1104 70-71 0.5938171562768275 1104 72-73 0.6836156258344701 1104 74-75 0.6761432553355533 1104 76-77 0.5386776525278592 1104 78-79 0.5442338333235668 1104 80-81 0.6745006877569537 1104 82-83 0.7079940602187165 1104 84-85 0.7329942905460527 1104 86-87 0.6667663861712114 1104 88-89 0.6445189668665066 1104 90-91 0.6931206190933246 1104 92-93 0.6832970003774825 1104 94-95 0.721729494627322 1104 96-97 1.0739896964231335 1104 98-99 0.7959989778735448 1104 100-101 0.8349663123518702 1105 1 0.12734177137363645 1105 2 0.11095923013246534 1105 3 0.11392589565758726 1105 4 0.02462122453634663 1105 5 0.06659556905765385 1105 6 0.03174035870875258 1105 7 0.06053407070685779 1105 8 0.1409176617439698 1105 9 0.13516645232516566 1105 10-11 0.11081003785006516 1105 12-13 0.12107361927359506 1105 14-15 0.12868254954361902 1105 16-17 0.13108556864453647 1105 18-19 0.06797425564217008 1105 20-21 0.10795505236334435 1105 22-23 0.15263123901247866 1105 24-25 0.1625770111911038 1105 26-27 0.11699887988471858 1105 28-29 0.14203559761733686 1105 30-31 0.05118827927304892 1105 32-33 0.12404334644180182 1105 34-35 0.10025228702585309 1105 36-37 0.13141558036977585 1105 38-39 0.11882451399790739 1105 40-41 0.21050253740721558 1105 42-43 0.10829847757653255 1105 44-45 0.22336937538441504 1105 46-47 0.20787187178591182 1105 48-49 0.18464999285236416 1105 50-51 0.11433112243513932 1105 52-53 0.15336685419075735 1105 54-55 0.262619640199496 1105 56-57 0.27644457001442646 1105 58-59 0.2249111237375132 1105 60-61 0.22673888413328314 1105 62-63 0.2829554564289367 1105 64-65 0.2115309575361124 1105 66-67 0.26323317157199 1105 68-69 0.21911350420339915 1105 70-71 0.20932771756961444 1105 72-73 0.2605468778701834 1105 74-75 0.29544515316849385 1105 76-77 0.30714044266265716 1105 78-79 0.2716177479022832 1105 80-81 0.23459467040895987 1105 82-83 0.2013940397747831 1105 84-85 0.28804852114942037 1105 86-87 0.19548490120523354 1105 88-89 0.2045225008099294 1105 90-91 0.26663212316714535 1105 92-93 0.24241293017453458 1105 94-95 0.28356561631946775 1105 96-97 0.6696526618946343 1105 98-99 0.43961783019140555 1105 100-101 0.39363269291008507 1106 1 -0.07657754969823927 1106 2 -0.10776499436379083 1106 3 -0.09736795745988047 1106 4 -0.06082496296983919 1106 5 -0.06333347742837958 1106 6 -0.05076082386938907 1106 7 -0.048875904208230736 1106 8 -0.025469869656745914 1106 9 -0.020487620397503292 1106 10-11 -0.07672787767651812 1106 12-13 -0.0863816847932668 1106 14-15 -0.18374170182609362 1106 16-17 -0.13649896270104023 1106 18-19 -0.12467899743266031 1106 20-21 -0.16482536804551984 1106 22-23 -0.13004139810878002 1106 24-25 -0.04616958513096847 1106 26-27 -0.06443426846310274 1106 28-29 -0.08817083636912315 1106 30-31 -0.21913026510218714 1106 32-33 -0.269801066366135 1106 34-35 -0.2953832237168541 1106 36-37 -0.22486774827339673 1106 38-39 -0.27332955033431716 1106 40-41 -0.2785817272139113 1106 42-43 -0.2188429325542245 1106 44-45 -0.2151063977423533 1106 46-47 -0.20692938554385165 1106 48-49 -0.27249194542552857 1106 50-51 -0.20593720845241137 1106 52-53 -0.11575233514759731 1106 54-55 -0.19586714985658915 1106 56-57 -0.16971751922844192 1106 58-59 -0.14944300188689397 1106 60-61 -0.26281181257537867 1106 62-63 -0.21181042363859603 1106 64-65 -0.13465357796010835 1106 66-67 -0.1748533573278266 1106 68-69 -0.12928768890938613 1106 70-71 -0.13826166421905128 1106 72-73 -0.09746091498034559 1106 74-75 -0.10003324020770066 1106 76-77 -0.18641855819337394 1106 78-79 -0.10831917113686984 1106 80-81 -0.1602424822925954 1106 82-83 -0.1622282569422424 1106 84-85 -0.17142623561445447 1106 86-87 -0.24476055325494173 1106 88-89 -0.2626200319271419 1106 90-91 -0.18724423789758404 1106 92-93 -0.14729309315510264 1106 94-95 -0.12743485031550605 1106 96-97 0.14290926925585623 1106 98-99 -0.06631178536088456 1106 100-101 -0.0879472973738693 1107 1 -0.20540802706891625 1107 2 -0.2693930739658583 1107 3 -0.22537401202094998 1107 4 -0.15765819769423928 1107 5 -0.15680322538793945 1107 6 -0.14544961720135774 1107 7 -0.19649721953346244 1107 8 -0.14302124935642269 1107 9 -0.21237920052885073 1107 10-11 -0.25491072250384406 1107 12-13 -0.1923253126003388 1107 14-15 -0.2471693644971822 1107 16-17 -0.27219160043615886 1107 18-19 -0.28862595890607423 1107 20-21 -0.30626533302593373 1107 22-23 -0.2002181694147822 1107 24-25 -0.12130933415265588 1107 26-27 -0.18216010345440736 1107 28-29 -0.20199841171047694 1107 30-31 -0.2580053399451785 1107 32-33 -0.32457088726784633 1107 34-35 -0.3117755467468797 1107 36-37 -0.37431517521849855 1107 38-39 -0.3463005355168889 1107 40-41 -0.3432332901184978 1107 42-43 -0.34945196168139603 1107 44-45 -0.21270384265500297 1107 46-47 -0.24277116712958247 1107 48-49 -0.3687390294290722 1107 50-51 -0.33847660712520167 1107 52-53 -0.36643883818002365 1107 54-55 -0.29825940296080233 1107 56-57 -0.31849519259701253 1107 58-59 -0.2971439342182123 1107 60-61 -0.3173382781648755 1107 62-63 -0.4309916519218575 1107 64-65 -0.3824709901725498 1107 66-67 -0.29525283825738313 1107 68-69 -0.3480825477702467 1107 70-71 -0.30714570094057336 1107 72-73 -0.24847685952915555 1107 74-75 -0.257355482660067 1107 76-77 -0.34990647422070964 1107 78-79 -0.1464642763256876 1107 80-81 -0.18386051491703626 1107 82-83 -0.21231498601738963 1107 84-85 -0.20616337891899406 1107 86-87 -0.2036644134123904 1107 88-89 -0.28013336743929074 1107 90-91 -0.25110111818276337 1107 92-93 -0.24056467810324378 1107 94-95 -0.2299920094600587 1107 96-97 0.04129402599043175 1107 98-99 -0.24802117510956379 1107 100-101 -0.3612012462768881 1108 1 -0.35702842869673646 1108 2 -0.3455297919121776 1108 3 -0.3554971573418797 1108 4 -0.23481528076711555 1108 5 -0.2723125184318249 1108 6 -0.22779155696100162 1108 7 -0.24678035438147816 1108 8 -0.22650336338539034 1108 9 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2305 18-19 -0.12684186894905025 2305 20-21 -0.12216784567325334 2305 22-23 -0.11557239233853522 2305 24-25 -0.030371273843130098 2305 26-27 -0.08763387688593127 2305 28-29 -0.10231471689636606 2305 30-31 -0.11162791754568246 2305 32-33 -0.11132726499094048 2305 34-35 -0.15684374320095884 2305 36-37 -0.058803475268113914 2305 38-39 -0.10055557639506674 2305 40-41 -0.13720395088967763 2305 42-43 -0.17265776420050827 2305 44-45 -0.18045525343890034 2305 46-47 -0.06910629900310994 2305 48-49 -0.11605580205178967 2305 50-51 -0.10746738407107159 2305 52-53 -0.15268854860553915 2305 54-55 -0.03283946592640774 2305 56-57 -0.10018940318689573 2305 58-59 -0.07318528317316719 2305 60-61 -0.06743621481690809 2305 62-63 -0.06780822214505378 2305 64-65 -0.05854653367266138 2305 66-67 -0.11243262890092609 2305 68-69 -0.07802934821870622 2305 70-71 -0.04762228582932693 2305 72-73 -0.05072437726508383 2305 74-75 -0.11366902806266665 2305 76-77 -0.0660060168329224 2305 78-79 -0.05668053523721994 2305 80-81 -0.05046329254806636 2305 82-83 1.2636559864631636E-4 2305 84-85 0.04959126167467076 2305 86-87 0.09724561695974643 2305 88-89 0.08006736820592408 2305 90-91 0.09255552170222359 2305 92-93 0.10182075482852682 2305 94-95 0.029305472312476155 2305 96-97 0.3254306054030174 2305 98-99 0.03986114234401228 2305 100-101 0.051802530484863496 2306 1 -0.2277118733448269 2306 2 -0.24638057667731772 2306 3 -0.23562460941298724 2306 4 -0.1724651551356331 2306 5 -0.17343433118042384 2306 6 -0.14107414169804144 2306 7 -0.18792055814442676 2306 8 -0.17453062932486318 2306 9 -0.34061465434167104 2306 10-11 -0.2627591498047863 2306 12-13 -0.2714203212451096 2306 14-15 -0.3491802698570794 2306 16-17 -0.39046681696910923 2306 18-19 -0.35694593682465126 2306 20-21 -0.43494207758289605 2306 22-23 -0.37028670073297576 2306 24-25 -0.3787456399229683 2306 26-27 -0.381006517636834 2306 28-29 -0.46223223274594005 2306 30-31 -0.5198572307323204 2306 32-33 -0.5400563526433189 2306 34-35 -0.5206371530763292 2306 36-37 -0.562000500763773 2306 38-39 -0.7206983116686487 2306 40-41 -0.5816211548676691 2306 42-43 -0.6783141909991315 2306 44-45 -0.6775561124943295 2306 46-47 -0.5881891832864596 2306 48-49 -0.5664770048881778 2306 50-51 -0.5507660634197649 2306 52-53 -0.4965722747619097 2306 54-55 -0.558221904556838 2306 56-57 -0.5797686446951076 2306 58-59 -0.5783458677735069 2306 60-61 -0.556747117044651 2306 62-63 -0.5039092530852898 2306 64-65 -0.5249819722043298 2306 66-67 -0.5093995405390572 2306 68-69 -0.566086543996775 2306 70-71 -0.5885649018427657 2306 72-73 -0.6140157268518394 2306 74-75 -0.5868127511422472 2306 76-77 -0.5615210400753412 2306 78-79 -0.4897602481881691 2306 80-81 -0.5081502055787794 2306 82-83 -0.45867165703546675 2306 84-85 -0.418676971765116 2306 86-87 -0.47763309660875564 2306 88-89 -0.4899345038539451 2306 90-91 -0.48776222600450225 2306 92-93 -0.5153123229573815 2306 94-95 -0.5625926825678249 2306 96-97 -0.2719802351511511 2306 98-99 -0.5032575470613807 2306 100-101 -0.5000615984137937 2307 1 -0.4680377752300444 2307 2 -0.462276148095242 2307 3 -0.4796819091859703 2307 4 -0.34989342527259737 2307 5 -0.38361547882070113 2307 6 -0.3961549764493384 2307 7 -0.3994047655014583 2307 8 -0.38378042556227143 2307 9 -0.4604184094768442 2307 10-11 -0.572117612865064 2307 12-13 -0.5707706365854719 2307 14-15 -0.8899614549120045 2307 16-17 -0.7471474894197172 2307 18-19 -0.6940921713226302 2307 20-21 -0.6447113672182425 2307 22-23 -0.6599179350899078 2307 24-25 -0.5971807893899097 2307 26-27 -0.681326644618764 2307 28-29 -0.68686099251115 2307 30-31 -0.7198383254245826 2307 32-33 -0.7490084560981245 2307 34-35 -0.7169681739733633 2307 36-37 -0.7883436131000536 2307 38-39 -0.8255428577908148 2307 40-41 -0.7325838684095842 2307 42-43 -0.8464345746155857 2307 44-45 -0.7807417963957519 2307 46-47 -0.8106791407761449 2307 48-49 -0.6807153464226801 2307 50-51 -0.7705709495760971 2307 52-53 -0.8614684084074966 2307 54-55 -0.8651920303501441 2307 56-57 -0.9193202297731418 2307 58-59 -0.8978801566991592 2307 60-61 -0.9806142436141627 2307 62-63 -0.9963796339499069 2307 64-65 -0.9801814300283596 2307 66-67 -0.9265763242231273 2307 68-69 -0.839717512725727 2307 70-71 -0.9344859754478776 2307 72-73 -1.2629346158174926 2307 74-75 -0.9922567934303714 2307 76-77 -0.9125414076184839 2307 78-79 -0.9344695220207058 2307 80-81 -0.8491544173018823 2307 82-83 -0.8662293913936665 2307 84-85 -0.861418958732223 2307 86-87 -0.8172174136261177 2307 88-89 -0.8394190452233801 2307 90-91 -0.8818382482212002 2307 92-93 -0.7991524134417567 2307 94-95 -0.8345354564396068 2307 96-97 -0.6381830269989948 2307 98-99 -1.004661966719599 2307 100-101 -1.020648735563885 2308 1 -1.4074980370529246 2308 2 -0.8816641568814774 2308 3 -0.8627177158039743 2308 4 -0.5835724321277738 2308 5 -0.6487600615440883 2308 6 -0.6661881066498339 2308 7 -0.6462281994299133 2308 8 -0.6104131064441702 2308 9 -0.8760238639261502 2308 10-11 -0.8479044668009337 2308 12-13 -0.7885691925383966 2308 14-15 -1.035009703374051 2308 16-17 -0.9767100279201699 2308 18-19 -0.9668121035902217 2308 20-21 -0.9903921160735081 2308 22-23 -1.0877007507625436 2308 24-25 -1.2480865677211597 2308 26-27 -1.2189340138813947 2308 28-29 -1.243003049748772 2308 30-31 -1.1715475360710244 2308 32-33 -1.2035566091189196 2308 34-35 -1.2709055252416093 2308 36-37 -1.369316672049166 2308 38-39 -1.3854026499167205 2308 40-41 -1.3066034934258042 2308 42-43 -1.1710090136724887 2308 44-45 -1.2097797154325391 2308 46-47 -1.257503137616439 2308 48-49 -1.3386107250191728 2308 50-51 -1.3609973005540184 2308 52-53 -1.2595042511895187 2308 54-55 -1.223169335677646 2308 56-57 -1.2102001208341946 2308 58-59 -1.146556888048707 2308 60-61 -1.0797645565853813 2308 62-63 -1.0879463530012146 2308 64-65 -1.0579175616773142 2308 66-67 -1.0908339449109477 2308 68-69 -1.1669085474576377 2308 70-71 -1.0861631932816422 2308 72-73 -1.1945289935152417 2308 74-75 -1.2513427078506325 2308 76-77 -1.1911383445581762 2308 78-79 -1.591224079479737 2308 80-81 -1.2651010521751793 2308 82-83 -1.1519407703468367 2308 84-85 -1.094712540153992 2308 86-87 -1.1247365765042403 2308 88-89 -1.1569346949893458 2308 90-91 -1.2136190700132978 2308 92-93 -1.1542819607644894 2308 94-95 -1.2485224756277589 2308 96-97 -1.0930569203445053 2308 98-99 -1.3367449926790833 2308 100-101 -1.4187898838784925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40.0 3 93.0 4 192.0 5 520.0 6 899.0 7 1786.0 8 3744.0 9 5680.0 10 7606.0 11 9429.0 12 11165.0 13 12856.0 14 14315.0 15 15963.0 16 18191.0 17 21398.0 18 24993.0 19 28860.0 20 30628.0 21 31260.0 22 34026.0 23 39744.0 24 48446.0 25 61344.0 26 78044.0 27 99636.0 28 127929.0 29 161001.0 30 200020.0 31 248115.0 32 304491.0 33 383412.0 34 506779.0 35 743006.0 36 1028240.0 37 1766930.0 38 2232135.0 39 718537.0 40 2882.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.640580436446662 31.024841412971938 29.697175494628947 22.637402655952453 2 21.890103659147513 29.937497284610902 28.232875758868126 19.939523297373455 3 20.98464951650188 28.763461593870442 28.704262718006348 21.54762617162133 4 21.133065294046663 28.803001762242143 28.780939106738373 21.28299383697282 5 21.76402516816062 28.827497083140013 29.062785099585465 20.345692649113907 6 21.81054892133326 28.875645684695883 28.052006048091076 21.26179934587978 7 22.027384843315325 28.936436867647313 28.52500488955696 20.511173399480406 8 21.262787288014444 29.069407919890157 28.50168613226857 21.166118659826832 9 20.90126813350904 28.763465378256292 29.12058056929419 21.214685918940482 10-11 20.967007541275894 29.196023862146074 28.454545404176596 21.382423192401433 12-13 20.962769001815644 29.27099891570958 28.317571322429853 21.448660760044923 14-15 21.166684304162022 29.34890493316128 28.121379580877704 21.36303118179899 16-17 21.316002785800837 29.104548977847124 28.131635184199165 21.447813052152874 18-19 21.30240067550684 29.121015565135828 28.130105985648807 21.446477773708533 20-21 21.38417955450457 29.050085130926544 28.160313197592952 21.405422116975934 22-23 21.316219145685736 28.99755222703348 28.22655277671445 21.45967585056634 24-25 21.32328858858478 28.96657810359934 28.144731825450652 21.565401482365225 26-27 21.528752064009222 28.858356328335383 28.28555359774815 21.327338009907244 28-29 21.483893273022332 28.90084421733014 28.19519665437952 21.420065855268007 30-31 21.46999751228207 28.901736249817855 28.149032587996786 21.47923364990329 32-33 21.52722056528265 28.953900758338424 28.04697520648336 21.471903469895565 34-35 21.535597913862905 28.95129668834324 28.07116535456629 21.44194004322756 36-37 21.57387220221767 28.951025200194806 28.021422077083795 21.453680520503728 38-39 21.556746286568483 28.943645155016963 28.029472531771038 21.470136026643512 40-41 21.563821600151147 28.9703451833293 27.985929157106863 21.47990405941269 42-43 21.585501868004677 28.940658785384187 27.98703727199843 21.486802074612697 44-45 21.58041008007792 28.95845511054277 27.954985048759823 21.50614976061948 46-47 21.59043417550624 28.97357140296606 27.961978802979875 21.474015618547828 48-49 21.574719910109717 28.980844571926905 27.91907658569856 21.525358932264815 50-51 21.606605916114592 28.992269236458974 27.88138405766187 21.519740789764562 52-53 21.594937466306384 29.017528715412272 27.850174001740847 21.537359816540498 54-55 21.605220772500132 29.046960246932326 27.773669749626983 21.574149230940563 56-57 21.64721832689057 29.065282926664405 27.723233900337256 21.564264846107775 58-59 21.62548265329246 29.134545647961872 27.69483291566636 21.545138783079306 60-61 21.586438347542334 29.116949206633446 27.75021586094832 21.546396584875904 62-63 21.591778395749753 29.121363860983895 27.727857435043727 21.55900030822262 64-65 21.594504619883576 29.145948317918023 27.731823189757222 21.527723872441182 66-67 21.60112340874437 29.17173260699975 27.680506675295202 21.546637308960676 68-69 21.57209607805407 29.172839115595394 27.713353327847777 21.541711478502755 70-71 21.64412747621884 29.224168007811336 27.618467810062334 21.513236705907488 72-73 21.560805241032323 29.242999673438323 27.65456024486696 21.54163484066239 74-75 21.613290834122818 29.238554087898766 27.63239508951612 21.515759988462293 76-77 21.60904759540453 29.275138889620855 27.620046704746837 21.495766810227778 78-79 21.643772919409994 29.26948322743655 27.581918362620083 21.504825490533374 80-81 21.625919313553357 29.308634985971533 27.578666933500923 21.486778766974187 82-83 21.57362397370868 29.362495108940827 27.555138623504412 21.50874229384608 84-85 21.560199800474365 29.411844889543875 27.5493045749264 21.47865073505536 86-87 21.572582143790257 29.452461637201054 27.526625000069338 21.448331218939348 88-89 21.566965636326245 29.4976758239475 27.491765670857525 21.443592868868734 90-91 21.56618073096883 29.477232091976095 27.492599765416475 21.4639874116386 92-93 21.566846521800432 29.543459898146146 27.438926779719363 21.450766800334055 94-95 21.561488448748957 29.580113250474092 27.444463624481113 21.413934676295835 96-97 21.520736301099998 29.624155015787917 27.420183915990844 21.434924767121238 98-99 21.557111474150158 29.617836990736407 27.3625959957063 21.462455539407138 100-101 21.570027573942692 29.68399550881778 27.293839827178974 21.452137090060557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 109431.0 1 62089.5 2 10792.0 3 6114.0 4 4893.5 5 4231.5 6 4145.0 7 4091.0 8 3686.5 9 3501.0 10 3617.5 11 4245.0 12 8024.0 13 7855.0 14 4672.0 15 4919.5 16 5512.0 17 6632.0 18 8240.0 19 10723.5 20 14221.0 21 18663.0 22 25105.0 23 34481.5 24 44159.5 25 55690.5 26 75154.5 27 96216.0 28 108046.0 29 117293.5 30 133658.0 31 154784.5 32 177757.5 33 201836.0 34 225775.5 35 243839.5 36 258272.5 37 273043.0 38 282372.0 39 285870.5 40 292127.0 41 301751.5 42 307484.5 43 311002.5 44 316281.5 45 321951.5 46 321882.0 47 319695.0 48 316643.5 49 311541.5 50 308278.5 51 300082.5 52 290712.0 53 282303.0 54 267738.5 55 248040.0 56 226084.5 57 203239.0 58 179476.5 59 149909.0 60 116147.0 61 87080.5 62 63594.0 63 45385.5 64 32213.0 65 21558.0 66 13213.5 67 8344.0 68 5252.0 69 3039.0 70 1607.5 71 757.5 72 380.0 73 206.5 74 118.5 75 85.0 76 59.0 77 33.0 78 21.0 79 17.0 80 15.5 81 14.5 82 9.0 83 3.5 84 4.5 85 4.5 86 3.0 87 2.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01170169325496006 2 0.018693898220755324 3 0.005706791691576166 4 3.9892136096454753E-4 5 8.421673175918225E-4 6 0.0 7 0.0 8 0.008033832963869359 9 0.010150332406764597 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0527091469897782E-4 24-25 0.004936651841936276 26-27 0.007036529561457992 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 5.540574457840938E-6 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 7.756804240977312E-5 62-63 1.4959551036170532E-4 64-65 3.047315951812516E-4 66-67 1.6621723373522814E-4 68-69 2.936504462655697E-4 70-71 1.8283895710875095E-4 72-73 6.648689349409125E-5 74-75 1.163520636146597E-4 76-77 0.12557712008696487 78-79 0.05647507544877268 80-81 7.202746795193219E-5 82-83 0.002393528165787285 84-85 0.010338711938331191 86-87 0.1187899163761097 88-89 0.1353728557284276 90-91 0.1492852381920662 92-93 0.14722968506820724 94-95 0.11450151174574083 96-97 1.2128262082469234 98-99 0.07689763290037438 100-101 0.06891920568108342 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9024335.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.627840844991844 #Duplication Level Percentage of deduplicated Percentage of total 1 88.22724285338852 53.49027237907683 2 6.454168134218465 7.826045568564299 3 1.766167762675519 3.2123681406314013 4 0.9028348360254388 2.189477069914584 5 0.5686742558963812 1.723874613956498 6 0.36843999203491573 1.340263271881376 7 0.24895142649249274 1.0565371224464375 8 0.18824567418057683 0.9130343019182561 9 0.1400913766943711 0.7644093920983916 >10 0.9695959830785578 12.799171308175012 >50 0.12854970186736628 5.3072295138878856 >100 0.036419022240045194 3.548838810096169 >500 2.0632706912827796E-4 0.08952392210162256 >1k 6.877568970942563E-5 0.12709311344468766 >5k 1.3755137941885126E-4 0.4804353192617097 >10k+ 2.0632706912827688E-4 5.131426152544961 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100047 1.1086357055672247 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12276 0.1360321840889107 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10741 0.11902262050333902 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1081148915681875E-5 0.0 0.0 0.0 1.1081148915681875E-5 2 2.216229783136375E-5 0.0 0.0 1.1081148915681875E-5 3.324344674704563E-5 3 3.324344674704563E-5 0.0 0.0 1.1081148915681875E-5 5.540574457840938E-5 4 4.43245956627275E-5 0.0 0.0 1.1081148915681875E-5 6.648689349409125E-5 5 4.43245956627275E-5 0.0 0.0 2.216229783136375E-5 7.756804240977314E-5 6 5.540574457840938E-5 0.0 0.0 3.324344674704563E-5 9.973034024113688E-5 7 5.540574457840938E-5 0.0 0.0 4.43245956627275E-5 9.973034024113688E-5 8 5.540574457840938E-5 0.0 0.0 4.43245956627275E-5 9.973034024113688E-5 9 7.756804240977314E-5 0.0 0.0 4.43245956627275E-5 1.2189263807250063E-4 10-11 9.973034024113688E-5 0.0 0.0 4.43245956627275E-5 1.2743321253034156E-4 12-13 1.1081148915681876E-4 0.0 0.0 5.540574457840938E-5 1.4959551036170532E-4 14-15 1.2189263807250064E-4 0.0 0.0 5.540574457840938E-5 1.7175780819306908E-4 16-17 1.4405493590386438E-4 0.0 0.0 5.540574457840938E-5 1.9392010602443282E-4 18-19 1.7175780819306908E-4 0.0 0.0 7.20274679519322E-5 1.9946068048227376E-4 20-21 2.050012549401147E-4 0.0 0.0 1.163520636146597E-4 1.9946068048227376E-4 22-23 2.327041272293194E-4 0.0 0.0 1.4405493590386438E-4 2.1054182939795566E-4 24-25 2.6040699951852407E-4 0.0 0.0 1.4405493590386438E-4 2.493258506028422E-4 26-27 2.7148814843420597E-4 0.0 0.0 1.6621723373522814E-4 2.548664250606831E-4 28-29 2.9919102072341065E-4 0.0 0.0 1.7729838265091E-4 2.6040699951852407E-4 30-31 3.5459676530182006E-4 0.0 0.0 1.883795315665919E-4 2.7148814843420597E-4 32-33 3.933807865067066E-4 0.0 0.0 2.050012549401147E-4 3.047315951812516E-4 34-35 4.3216480771159316E-4 0.0 0.0 2.2162297831363753E-4 3.213533185547744E-4 36-37 4.4324595662727505E-4 0.0 0.0 2.2162297831363753E-4 3.324344674704563E-4 38-39 4.875705522900025E-4 0.0 0.0 2.327041272293194E-4 3.379750419282972E-4 40-41 5.263545734948891E-4 0.0 0.0 2.327041272293194E-4 3.490561908439791E-4 42-43 5.540574457840938E-4 0.0 0.0 2.327041272293194E-4 3.8784021204886564E-4 44-45 6.039226159046621E-4 0.0 0.0 2.327041272293194E-4 4.044619354223885E-4 46-47 6.371660626517078E-4 0.0 0.0 2.493258506028422E-4 4.210836587959113E-4 48-49 6.482472115673897E-4 0.0 0.0 2.6040699951852407E-4 4.4324595662727505E-4 50-51 6.759500838565944E-4 0.0 0.0 2.936504462655697E-4 4.543271055429569E-4 52-53 7.14734105061481E-4 0.0 0.0 2.9919102072341065E-4 4.543271055429569E-4 54-55 7.701398496398903E-4 0.0 0.0 3.1027216963909254E-4 4.543271055429569E-4 56-57 8.255455942182998E-4 0.0 0.0 3.1581274409693343E-4 4.654082544586388E-4 58-59 8.80951338796709E-4 0.0 0.0 3.213533185547744E-4 4.7094882891647973E-4 60-61 9.197353600015957E-4 0.0 0.0 3.379750419282972E-4 4.764894033743207E-4 62-63 0.0010527091469897782 0.0 0.0 3.4351561638613817E-4 4.764894033743207E-4 64-65 0.0011191960404838695 0.0 0.0 3.490561908439791E-4 4.820299778321616E-4 66-67 0.001157980061688756 0.0 0.0 3.5459676530182E-4 5.097328501213663E-4 68-69 0.0012244669551828473 0.0 0.0 3.5459676530182E-4 5.097328501213663E-4 70-71 0.0013020349975926204 0.0 0.0 3.8784021204886564E-4 5.208139990370481E-4 72-73 0.001423927635665121 0.0 0.0 3.8784021204886564E-4 5.263545734948891E-4 74-75 0.0015956854438581901 0.0 0.0 3.933807865067066E-4 5.485168713262529E-4 76-77 0.0017674432520512593 0.0 0.0 4.044619354223885E-4 5.595980202419348E-4 78-79 0.002044471974943306 0.0 0.0 4.3216480771159316E-4 5.651385946997756E-4 80-81 0.002293797825546148 0.0 0.0 4.377053821694341E-4 5.651385946997756E-4 82-83 0.002581907697353877 1.1081148915681875E-5 0.0 4.543271055429569E-4 5.706791691576166E-4 84-85 0.003102721696390925 1.1081148915681875E-5 0.0 4.7094882891647973E-4 5.817603180732985E-4 86-87 0.003817455801452406 1.1081148915681875E-5 0.0 4.875705522900025E-4 5.928414669889803E-4 88-89 0.00502530103326173 1.1081148915681875E-5 0.0 4.986517012056844E-4 6.150037648203441E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 480 0.0 24.698092 3 CGCGTAG 245 0.0 23.219528 54-55 ACTCGCG 270 0.0 21.069515 50-51 CAACGCA 6905 0.0 20.28761 12-13 ATCAACG 7090 0.0 19.791674 10-11 CGTACGC 325 0.0 18.23576 20-21 CGTAGCG 325 0.0 18.233335 56-57 CTCGCGT 325 0.0 18.233234 52-53 TCAACGC 6660 0.0 17.581656 10-11 ACGCAGA 8085 0.0 17.4146 14-15 GTATCAA 9055 0.0 16.44093 9 TCGCGTA 390 0.0 16.40991 52-53 AGTGGTA 9560 0.0 15.723061 5 AACGCAG 7540 0.0 15.592559 12-13 ACGTACG 400 0.0 15.409217 20-21 TGGTATC 9665 0.0 15.405064 7 CGCTAGT 365 0.0 15.314013 90-91 GGTATCA 9695 0.0 15.161759 8 AAGCAGT 10465 0.0 14.772453 1 GTGGTAT 10225 0.0 14.6077385 6 >>END_MODULE