FastQCFastQC Report
Sat 18 Jun 2016
SRR578583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7151159
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82878311.58949199703153No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT212590.29728048278607705No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG90840.12702836001828513No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT73310.1025148510891731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1870100.090.883165
AAGCAGT1880200.090.81141
TGGTATC1873300.090.805297
GTGGTAT1874600.090.786686
GTATCAA1879350.090.554669
GGTATCA1880900.090.503418
AGCAGTG1890400.090.215672
GCAGTGG1891550.090.057093
CAGTGGT1894200.089.618374
TCAACGC1862650.046.09153412-13
ATCAACG1865450.046.0112310-11
CAACGCA1866950.046.00700812-13
ACGCAGA1875350.045.86059614-15
CGCAGAG1879050.045.84745416-17
AACGCAG1875950.045.839614-15
TATCAAC1878500.045.64226510-11
AGAGTAC1825000.045.636518-19
CAGAGTA1876650.045.30683518-19
GAGTACT1111850.045.2236120-21
GCAGAGT1901450.045.05753316-17
Produced by FastQC (version 0.11.5)