FastQCFastQC Report
Sat 18 Jun 2016
SRR578583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7151159
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97960.13698478806022912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3300.025.907193
CTCGCGT1850.021.82623552-53
CGCGTAG1850.021.82425154-55
TCGCGTA1950.020.7069452-53
ACTCGCG2050.019.69753650-51
CGTAGCG2150.018.77756156-57
CGTACGC2300.018.58546420-21
GCGACGA2553.6379788E-1215.83261660-61
CAACGCA57000.015.62352812-13
TCAACGC54250.015.3211910-11
ATCAACG58550.015.12885810-11
CGACGAC3050.014.7944160-61
GATCGGA6200.014.5763811
CGCTAGT2801.6370905E-1114.41878890-91
ACGCAGA62900.014.19599814-15
AACGCAG60300.013.7839412-13
ACGTACG2954.0017767E-1113.68534320-21
CGCAGAG63600.013.29295714-15
GTATCAA78350.012.7908439
AGCGACG3201.5279511E-1012.61648358-59
Produced by FastQC (version 0.11.5)