##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578583_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7151159 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50990839387014 31.0 31.0 34.0 30.0 34.0 2 31.824480339480637 31.0 31.0 34.0 30.0 34.0 3 31.897727347413195 31.0 31.0 34.0 30.0 34.0 4 35.56688433301511 37.0 35.0 37.0 33.0 37.0 5 35.388954853332166 37.0 35.0 37.0 33.0 37.0 6 35.389530564206446 37.0 35.0 37.0 33.0 37.0 7 35.36771591849657 37.0 35.0 37.0 33.0 37.0 8 35.355663606416805 37.0 35.0 37.0 32.0 37.0 9 36.93032541997738 39.0 37.0 39.0 33.0 39.0 10-11 36.89765183517805 39.0 37.0 39.0 33.0 39.0 12-13 36.87958972804269 39.0 37.0 39.0 33.0 39.0 14-15 38.069767991454256 40.0 37.0 41.0 33.0 41.0 16-17 38.02559983633422 40.0 37.0 41.0 33.0 41.0 18-19 37.988207715700355 40.0 37.0 41.0 33.0 41.0 20-21 37.94375729864208 40.0 37.0 41.0 33.0 41.0 22-23 37.85870045680707 40.0 37.0 41.0 32.0 41.0 24-25 37.77362166048888 40.0 37.0 41.0 32.0 41.0 26-27 37.592680640998196 39.5 36.5 41.0 32.0 41.0 28-29 37.42472087391708 39.0 36.0 41.0 32.0 41.0 30-31 37.296930119998734 39.0 36.0 41.0 31.0 41.0 32-33 37.165753900871174 39.0 36.0 41.0 31.0 41.0 34-35 37.01148471177889 39.0 36.0 40.5 30.5 41.0 36-37 36.86437366306637 39.0 36.0 40.0 30.0 41.0 38-39 36.702416069898604 39.0 35.0 40.0 30.0 41.0 40-41 36.5348200900022 38.5 35.0 40.0 30.0 41.0 42-43 36.479165055622445 38.5 35.0 40.0 30.0 41.0 44-45 36.5181094421198 39.0 35.0 40.0 30.0 41.0 46-47 36.39416911300671 38.5 35.0 40.0 30.0 41.0 48-49 36.25805264573197 38.5 35.0 40.0 30.0 41.0 50-51 36.069362462784 38.0 35.0 40.0 29.0 41.0 52-53 36.12796478165288 38.5 35.0 40.0 29.0 41.0 54-55 36.12352927686267 39.0 35.0 40.0 29.0 41.0 56-57 35.972846499427575 38.0 35.0 40.0 28.5 41.0 58-59 35.77860462898392 38.0 34.0 40.0 28.0 41.0 60-61 35.53384800981212 38.0 34.0 40.0 28.0 41.0 62-63 35.26695882443671 38.0 34.0 40.0 28.0 41.0 64-65 34.96078300035002 37.0 34.0 40.0 27.5 41.0 66-67 34.627695314843365 37.0 33.0 40.0 26.5 41.0 68-69 34.268199252736515 36.0 33.0 39.0 26.0 41.0 70-71 33.894165477232434 36.0 32.5 39.0 26.0 40.5 72-73 33.50569844692308 35.5 32.0 39.0 26.0 40.0 74-75 33.09582292884272 35.0 32.0 38.0 25.5 40.0 76-77 31.723299398041632 34.0 30.5 36.0 23.5 39.0 78-79 32.36096904012342 35.0 31.0 37.0 25.0 39.0 80-81 32.2287906757492 35.0 31.5 36.0 25.0 39.0 82-83 31.9090573989475 35.0 31.0 36.0 24.5 37.5 84-85 31.5705642959414 34.5 31.0 35.5 24.0 37.0 86-87 31.210093496732487 34.0 31.0 35.0 23.0 37.0 88-89 30.880767369317336 34.0 31.0 35.0 20.5 36.0 90-91 30.552470375781045 34.0 31.0 35.0 20.0 36.0 92-93 30.189973051920674 34.0 30.0 35.0 18.5 36.0 94-95 29.82326899737511 34.0 30.0 35.0 16.0 35.0 96-97 29.44306524299068 34.0 30.0 35.0 4.5 35.0 98-99 28.97661455996154 34.0 29.5 35.0 2.0 35.0 100-101 27.8617652047731 33.0 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3324486919919316 1101 2 0.395919824996799 1101 3 0.35162631885703277 1101 4 0.2540674755223691 1101 5 0.265643971732473 1101 6 0.2612845431948614 1101 7 0.24032319175569228 1101 8 0.22068093417252044 1101 9 0.2860123430934962 1101 10-11 0.3359459783578984 1101 12-13 0.3051510119154415 1101 14-15 0.41489236895962023 1101 16-17 0.3562573667884692 1101 18-19 0.3305834011377229 1101 20-21 0.42751952397158277 1101 22-23 0.5092876364784544 1101 24-25 0.47552841893769227 1101 26-27 0.44881477614070775 1101 28-29 0.4601089494422368 1101 30-31 0.4649045618445342 1101 32-33 0.4348252118389482 1101 34-35 0.37503905768308243 1101 36-37 0.4513835554960224 1101 38-39 0.4101669513170094 1101 40-41 0.45969488185007634 1101 42-43 0.45544090721752895 1101 44-45 0.44703161028738236 1101 46-47 0.4161256544811991 1101 48-49 0.3228812071580407 1101 50-51 0.38621665261074867 1101 52-53 0.4336039635081548 1101 54-55 0.43198291685548185 1101 56-57 0.4474201675504048 1101 58-59 0.40625317308217745 1101 60-61 0.4867675724791525 1101 62-63 0.42505217202479884 1101 64-65 0.460810208648347 1101 66-67 0.4626738020045167 1101 68-69 0.4940970995039322 1101 70-71 0.4998117465943963 1101 72-73 0.42907812251253574 1101 74-75 0.39822762121511346 1101 76-77 0.3945057996368213 1101 78-79 0.5589666491170178 1101 80-81 0.5814132276539965 1101 82-83 0.57028141881813 1101 84-85 0.5569737292233654 1101 86-87 0.42982877413735565 1101 88-89 0.4649085453423041 1101 90-91 0.5938981441916518 1101 92-93 0.5435906790926985 1101 94-95 0.6105938352756901 1101 96-97 0.5268851578361726 1101 98-99 0.6346745294177119 1101 100-101 0.8235931034566804 1102 1 0.2721575366101874 1102 2 0.26524375684741486 1102 3 0.3081210428782519 1102 4 0.2386647823510728 1102 5 0.2818693980052558 1102 6 0.13255537037681364 1102 7 0.20430065695824595 1102 8 0.13698451340358275 1102 9 0.32671293082506025 1102 10-11 0.21690486525046282 1102 12-13 0.2128403908751153 1102 14-15 0.37407349069344065 1102 16-17 0.33063994145992126 1102 18-19 0.34796593695072175 1102 20-21 0.3855753981523904 1102 22-23 0.3519496066675245 1102 24-25 0.2955124840679346 1102 26-27 0.3528538838228883 1102 28-29 0.32741008117265125 1102 30-31 0.28361222879026116 1102 32-33 0.3669468916827654 1102 34-35 0.39905696861622886 1102 36-37 0.3342999293111717 1102 38-39 0.36191941391042803 1102 40-41 0.3115351587160191 1102 42-43 0.35028484695224194 1102 44-45 0.3234976953435833 1102 46-47 0.323738876701988 1102 48-49 0.3603827370276349 1102 50-51 0.30224293177311523 1102 52-53 0.31921353768645133 1102 54-55 0.36284216236013833 1102 56-57 0.396799561352843 1102 58-59 0.38093174057576107 1102 60-61 0.2751545758505358 1102 62-63 0.2946535686806655 1102 64-65 0.33179509193782764 1102 66-67 0.3343722852750872 1102 68-69 0.22132860387715425 1102 70-71 0.2752713010091483 1102 72-73 0.27563541444517625 1102 74-75 0.19565136442300002 1102 76-77 0.21376470415619409 1102 78-79 0.28012163258667044 1102 80-81 0.2509270619873689 1102 82-83 0.2614338449950715 1102 84-85 0.19819498136264002 1102 86-87 0.22382439815311628 1102 88-89 0.3724247086628978 1102 90-91 0.4135923505137207 1102 92-93 0.3614022761001898 1102 94-95 0.37223390199593 1102 96-97 0.31887292430602443 1102 98-99 0.3736114960770678 1102 100-101 0.599179253066346 1103 1 0.2790543085614132 1103 2 0.2929898613773112 1103 3 0.32403650669939665 1103 4 0.13749515787768019 1103 5 0.23515143193839094 1103 6 0.1856658458118332 1103 7 0.17259051908667544 1103 8 0.17101659164943328 1103 9 0.3845386501605432 1103 10-11 0.32637584770632344 1103 12-13 0.32585787207055716 1103 14-15 0.3889074464103359 1103 16-17 0.38496400699369104 1103 18-19 0.3717094109478225 1103 20-21 0.3626527392409571 1103 22-23 0.41704580576979566 1103 24-25 0.40782836644184073 1103 26-27 0.37295385702699235 1103 28-29 0.3854414207960062 1103 30-31 0.4261103741350638 1103 32-33 0.4013147284675469 1103 34-35 0.44454769210067724 1103 36-37 0.47169213832468415 1103 38-39 0.5119464643399283 1103 40-41 0.47374068737027386 1103 42-43 0.49028486546408345 1103 44-45 0.3562086759667764 1103 46-47 0.43130091798432346 1103 48-49 0.3963039950554119 1103 50-51 0.4550997949692146 1103 52-53 0.45012752750216833 1103 54-55 0.48643920768499527 1103 56-57 0.39404615621515404 1103 58-59 0.3874638292345338 1103 60-61 0.49628209513872434 1103 62-63 0.42715411593700026 1103 64-65 0.482509163113086 1103 66-67 0.4922030490875784 1103 68-69 0.47458208329360474 1103 70-71 0.46829390998490084 1103 72-73 0.5453703572597846 1103 74-75 0.5187618722537479 1103 76-77 0.4226435390578622 1103 78-79 0.5179687238440351 1103 80-81 0.5457235219677727 1103 82-83 0.458825017345422 1103 84-85 0.5271542131952316 1103 86-87 0.5974764930688856 1103 88-89 0.5758919304380079 1103 90-91 0.5491853472986712 1103 92-93 0.5223727409186658 1103 94-95 0.5464714339230738 1103 96-97 0.6032578614050976 1103 98-99 0.7264952477240456 1103 100-101 0.9288982109051673 1104 1 0.18958253792826696 1104 2 0.1956055394078966 1104 3 0.23117123439277876 1104 4 0.20233362916627584 1104 5 0.24323137573389175 1104 6 0.22953433383967337 1104 7 0.15778052365192252 1104 8 0.12031891044960474 1104 9 0.2478857156896339 1104 10-11 0.27628745865599313 1104 12-13 0.2953491156315948 1104 14-15 0.3244228046912525 1104 16-17 0.3260435926768537 1104 18-19 0.34521087483452106 1104 20-21 0.36493331342351354 1104 22-23 0.35349763237052656 1104 24-25 0.36046819199648183 1104 26-27 0.3924706605144408 1104 28-29 0.42085326365302933 1104 30-31 0.360232101348231 1104 32-33 0.3318951073741019 1104 34-35 0.3198075057911396 1104 36-37 0.4120815148720567 1104 38-39 0.42987645656715756 1104 40-41 0.460237984214416 1104 42-43 0.3807203956753682 1104 44-45 0.36748720991135997 1104 46-47 0.34281892246938384 1104 48-49 0.27271514446844236 1104 50-51 0.2628386663777249 1104 52-53 0.3655858611772018 1104 54-55 0.35410047897467933 1104 56-57 0.3744253361287875 1104 58-59 0.4428107289202501 1104 60-61 0.42236861981794505 1104 62-63 0.2998911512224538 1104 64-65 0.436523757329887 1104 66-67 0.4324642159506169 1104 68-69 0.35896733744857556 1104 70-71 0.24409503826657186 1104 72-73 0.35113478130489284 1104 74-75 0.4483048188530887 1104 76-77 0.3998044463122419 1104 78-79 0.43545795541514565 1104 80-81 0.4626081997714522 1104 82-83 0.33327274524565453 1104 84-85 0.39497262563676827 1104 86-87 0.5017262294963807 1104 88-89 0.43638630770981024 1104 90-91 0.581841775415139 1104 92-93 0.605431749266419 1104 94-95 0.5430550604437983 1104 96-97 0.5080890975487442 1104 98-99 0.6169767037924068 1104 100-101 0.8295809479769005 1105 1 0.11256441771917025 1105 2 0.10952677092529228 1105 3 0.11153587872536619 1105 4 0.15655441015239546 1105 5 0.07497866063864222 1105 6 0.13005707191277338 1105 7 0.13048939867217513 1105 8 0.10952149293009938 1105 9 0.1548709622312643 1105 10-11 0.11949017815542362 1105 12-13 0.11913304548660619 1105 14-15 0.17206406739676794 1105 16-17 0.15017724702595103 1105 18-19 0.1917196108784438 1105 20-21 0.1654827484379453 1105 22-23 0.2262837708401122 1105 24-25 0.13852955907561437 1105 26-27 0.18701241376945177 1105 28-29 0.13858329504775924 1105 30-31 0.1466320487155599 1105 32-33 0.20949697170143367 1105 34-35 0.20333587360212846 1105 36-37 0.2325623099298042 1105 38-39 0.28479929642574575 1105 40-41 0.27370694453438915 1105 42-43 0.23189696413427185 1105 44-45 0.1414902069284807 1105 46-47 0.20795375017895168 1105 48-49 0.2620700789440278 1105 50-51 0.20996540127111274 1105 52-53 0.12018920770132979 1105 54-55 0.19673119883513834 1105 56-57 0.20965181427481383 1105 58-59 0.13521944549877674 1105 60-61 0.04850737214101741 1105 62-63 0.04508961661049682 1105 64-65 0.09492318054546445 1105 66-67 0.14282741397232712 1105 68-69 0.10104149948541163 1105 70-71 0.10254426677389716 1105 72-73 0.15941251866570383 1105 74-75 0.15409473839252996 1105 76-77 0.08131276368721885 1105 78-79 0.13016059716176898 1105 80-81 0.15419072485624952 1105 82-83 0.1868525277770452 1105 84-85 0.22634604461780583 1105 86-87 0.2007027198762401 1105 88-89 0.17119939105972648 1105 90-91 0.1602207871607071 1105 92-93 0.17688945663531896 1105 94-95 0.1975074686601488 1105 96-97 0.23787451068799115 1105 98-99 0.4024332323635704 1105 100-101 0.513666471709989 1106 1 -0.05264184986211973 1106 2 0.03265859454108977 1106 3 -0.0016023234684254817 1106 4 0.04600907631105855 1106 5 0.011344524280652024 1106 6 0.12675856445172684 1106 7 0.09904446909283848 1106 8 0.06519635822640879 1106 9 -0.08263782052508617 1106 10-11 -0.03307333842015936 1106 12-13 -0.07283514921775947 1106 14-15 0.1789005396907868 1106 16-17 0.10365130533499922 1106 18-19 -0.003003413834242963 1106 20-21 0.046618425105613426 1106 22-23 0.047861382744862624 1106 24-25 0.1586140667270044 1106 26-27 0.19232045869194536 1106 28-29 0.14255319421881296 1106 30-31 0.10773718482646188 1106 32-33 0.08842986557039723 1106 34-35 0.026451682168392665 1106 36-37 0.02372161840018805 1106 38-39 0.0323900138878912 1106 40-41 -0.048912304194040246 1106 42-43 -0.01708867621689336 1106 44-45 -0.004279866135931343 1106 46-47 0.07741729321943325 1106 48-49 0.15746123527781464 1106 50-51 0.05154217674900963 1106 52-53 -0.08334094169965311 1106 54-55 -0.14037406833792687 1106 56-57 -0.1008189107305455 1106 58-59 0.02753429083499981 1106 60-61 0.126651977610436 1106 62-63 0.11711974422267701 1106 64-65 0.12517688790868675 1106 66-67 -0.030680911493433882 1106 68-69 0.02824335749460971 1106 70-71 -0.030382757398285776 1106 72-73 -0.10138677280830422 1106 74-75 -0.06610250576760279 1106 76-77 -0.08560570917298449 1106 78-79 -0.10028745870013012 1106 80-81 0.03349386383888486 1106 82-83 0.09553417197269809 1106 84-85 0.26526335592039985 1106 86-87 0.48282194999911 1106 88-89 0.3658564181813624 1106 90-91 0.37115825371598277 1106 92-93 0.21658595113965262 1106 94-95 0.3432615431401196 1106 96-97 0.3584093277240079 1106 98-99 0.43303588998365683 1106 100-101 0.3597582782284334 1107 1 0.13324831972552786 1107 2 0.10722814056978791 1107 3 0.0865495563496026 1107 4 0.1633149612019622 1107 5 0.015349170852530847 1107 6 0.06458323717357928 1107 7 0.1500925190067548 1107 8 0.07465966680487668 1107 9 -0.04966213180400558 1107 10-11 0.0032037711181303052 1107 12-13 -0.025830756874256622 1107 14-15 0.041616063392389435 1107 16-17 0.16914827837643998 1107 18-19 0.08380764053679002 1107 20-21 0.09954280127059434 1107 22-23 0.035910405797743294 1107 24-25 -2.380257768663796E-8 1107 26-27 -0.008142718491399137 1107 28-29 0.08068559356181026 1107 30-31 0.12522785762293154 1107 32-33 0.10401073326115551 1107 34-35 0.19216211016668439 1107 36-37 0.14948036891571093 1107 38-39 0.1780408970709786 1107 40-41 0.1648734373208498 1107 42-43 0.10365561037882998 1107 44-45 0.12445353334361187 1107 46-47 0.16600127905527984 1107 48-49 0.16467112022333907 1107 50-51 0.2797036008111533 1107 52-53 0.23196693301135696 1107 54-55 0.2166301111208142 1107 56-57 0.22619942225808387 1107 58-59 0.2243627601090168 1107 60-61 0.37043372722324364 1107 62-63 0.318572281439927 1107 64-65 0.31864874898499096 1107 66-67 0.1415643726488227 1107 68-69 0.3550351049515754 1107 70-71 0.36019140066884603 1107 72-73 0.30506873147913893 1107 74-75 0.284101292514805 1107 76-77 0.26211766883237075 1107 78-79 0.3009647737969843 1107 80-81 0.4116450520942436 1107 82-83 0.5093972616176821 1107 84-85 0.4266933345026942 1107 86-87 0.5061881617841273 1107 88-89 0.42799481683501384 1107 90-91 0.45132607912102074 1107 92-93 0.5097609157272309 1107 94-95 0.5148585029169261 1107 96-97 0.6334161955365651 1107 98-99 0.6438813997980652 1107 100-101 0.8675220567924598 1108 1 -0.03805146845226304 1108 2 0.011348645989464501 1108 3 -0.01624961563977223 1108 4 0.0480910571564479 1108 5 -0.052754266736592115 1108 6 -0.020553831605589323 1108 7 0.019036169110421497 1108 8 -0.023474308366104424 1108 9 -0.11928937975811493 1108 10-11 -0.1669063763969234 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-1.7082796620661327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 138.0 3 52.0 4 157.0 5 406.0 6 775.0 7 1550.0 8 3038.0 9 5192.0 10 6829.0 11 8600.0 12 10797.0 13 12521.0 14 14618.0 15 16382.0 16 18824.0 17 21982.0 18 25662.0 19 29172.0 20 31436.0 21 32788.0 22 36679.0 23 43682.0 24 53457.0 25 67518.0 26 86104.0 27 109480.0 28 137777.0 29 171578.0 30 209839.0 31 255670.0 32 311334.0 33 383939.0 34 493583.0 35 669462.0 36 888818.0 37 1298659.0 38 1347139.0 39 344763.0 40 759.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.556959419433355 31.71132438265078 29.030054855257465 21.7016613426584 2 21.95493066228845 30.090339202358667 28.629918590818637 19.32481154453425 3 20.955050782677326 29.064211829159444 28.956788682785543 21.023948705377688 4 21.14567612710225 29.260272547359044 29.022638256645592 20.571413068893108 5 21.519575015476573 29.298568296399548 28.90254702368059 20.279309664443286 6 21.373885360205183 29.20093015359784 28.482842514491175 20.9423419717058 7 21.49201569871621 29.259401064834535 28.813920766398954 20.434662470050306 8 20.899265656161965 29.174163134132623 28.839391616392675 21.087179593312737 9 20.699806912012956 29.059784000993403 29.29538266884292 20.945026418150718 10-11 20.71409203916964 29.518723629245137 28.800825209815873 20.966359121769347 12-13 20.72049729568274 29.469080392294504 28.738324618194707 21.07209769382805 14-15 20.882455618904267 29.520265842240807 28.579229505261278 21.01804903359365 16-17 21.008702366805487 29.314105491725957 28.571890035152347 21.105302106316206 18-19 21.025217174617033 29.32546029608072 28.511235522974044 21.138087006328206 20-21 21.073475843685628 29.27148950665718 28.556485926115695 21.098548723541505 22-23 21.051389231491225 29.225622235758507 28.58791343711527 21.135075095634996 24-25 21.119247942885902 29.177403271273928 28.488438028017555 21.214910757822615 26-27 21.27095211216476 29.10068347667025 28.579418285862353 21.048946125302635 28-29 21.25407030036018 29.09859641632468 28.533241669051623 21.11409161426352 30-31 21.230687693437705 29.1360944925163 28.467450724841868 21.165767089204127 32-33 21.295920158128745 29.14845685335265 28.396240596772397 21.159382391746206 34-35 21.306083973598337 29.16180293199385 28.42524909138619 21.106864003021617 36-37 21.339728488159164 29.136034617123446 28.371289736479888 21.152947158237502 38-39 21.347580608509908 29.11956593938674 28.363987232688793 21.168866219414564 40-41 21.36002780061866 29.150458961923782 28.327173286793865 21.162339950663693 42-43 21.38298365493454 29.122451264092465 28.351341122371277 21.14322395860172 44-45 21.34300798622427 29.157895612670252 28.325795942779088 21.17330045832639 46-47 21.422589004810572 29.152934345391486 28.287754403094855 21.136722246703087 48-49 21.370523531072237 29.192852683243498 28.253201249922473 21.183422535761792 50-51 21.426230483689356 29.192891251128305 28.203310368765592 21.17756789641674 52-53 21.398573985938445 29.21874380505785 28.18398961581726 21.19869259318644 54-55 21.425973823835694 29.197916294477338 28.158909361215084 21.217200520471888 56-57 21.407844518629776 29.21852248006232 28.117945636504516 21.255687364803382 58-59 21.41492378399547 29.253006734366217 28.082063101550514 21.250006380087804 60-61 21.379911554816214 29.24720210720147 28.13484814076052 21.238038197221794 62-63 21.384348970574674 29.27320357570089 28.082582802103985 21.259864651620454 64-65 21.396137459538693 29.296344734690255 28.11313188943414 21.194385916336913 66-67 21.422194801482732 29.30767729120881 28.0343096098567 21.235818297451754 68-69 21.382871121938983 29.346772584274877 28.053040283492326 21.21731601029381 70-71 21.451917087367143 29.384220080646678 27.991113776757448 21.17274905522873 72-73 21.39480078999062 29.34035112154699 28.009825518800174 21.25502256966222 74-75 21.439731040224334 29.407302212601643 28.001177160485586 21.151789586688437 76-77 21.434986972041877 29.394396069224584 27.98034556356529 21.190271395168253 78-79 21.414542733562488 29.404772009684027 27.98472946832814 21.195955788425344 80-81 21.4314520837275 29.431800559098807 27.963729195481957 21.173018161691733 82-83 21.390652922759426 29.476253766092963 27.94287683600856 21.19021647513905 84-85 21.42175084488189 29.501216413170056 27.918043922321555 21.1589888196265 86-87 21.391928478379082 29.547278045950915 27.897288985046963 21.163504490623037 88-89 21.401361102625554 29.586114076685767 27.864022025885603 21.14850279480308 90-91 21.401690015581483 29.589724962567036 27.858736763869423 21.149848257982057 92-93 21.398556660160693 29.605547940201 27.841125641463982 21.15476975817433 94-95 21.413351570061625 29.66143213739522 27.807712802014056 21.117503490529092 96-97 21.357105759880387 29.685190473167296 27.836708997766213 21.120994769186108 98-99 21.410746146184138 29.702409078024978 27.73663681649366 21.150207959297227 100-101 21.43711250162386 29.684083377253952 27.69952395129237 21.179280169829813 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 69708.0 1 39040.5 2 6333.5 3 3828.5 4 3037.0 5 2706.0 6 2702.0 7 2631.5 8 2435.5 9 2258.5 10 2319.0 11 2750.5 12 4784.5 13 4746.0 14 3130.5 15 3551.5 16 4201.5 17 5107.5 18 6532.5 19 8581.5 20 11653.5 21 16057.5 22 21790.0 23 30148.5 24 38963.5 25 49091.0 26 69133.0 27 90244.0 28 99153.0 29 105291.0 30 117344.5 31 133894.5 32 152390.0 33 171545.5 34 191440.5 35 206251.0 36 217974.5 37 228510.0 38 234295.0 39 235804.0 40 239881.5 41 247745.0 42 252464.0 43 255198.5 44 257069.0 45 258880.5 46 259444.0 47 258613.0 48 255146.0 49 248672.5 50 242141.5 51 230533.0 52 218566.5 53 206946.0 54 191459.0 55 174023.5 56 155320.5 57 136678.5 58 117964.0 59 98485.0 60 77512.0 61 58527.0 62 42691.5 63 30580.0 64 22519.5 65 16196.5 66 10736.0 67 7290.5 68 5048.5 69 3447.0 70 2214.5 71 1181.0 72 606.5 73 331.0 74 175.5 75 108.5 76 79.5 77 47.5 78 31.5 79 26.0 80 17.0 81 12.5 82 8.5 83 6.5 84 8.5 85 5.0 86 2.0 87 2.5 88 2.0 89 2.0 90 2.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14751454973941988 2 0.0 3 0.0 4 0.003565855548729933 5 3.915449229977966E-4 6 0.0011466672744935471 7 1.3983747249921306E-5 8 1.81788714248977E-4 9 2.0975620874881962E-4 10-11 1.1186997799937045E-4 12-13 1.3983747249921306E-5 14-15 6.991873624960653E-6 16-17 1.3983747249921306E-5 18-19 1.3983747249921306E-5 20-21 8.390248349952783E-5 22-23 1.5382121974913437E-4 24-25 0.0 26-27 6.991873624960653E-6 28-29 0.005978051949341359 30-31 0.0016291065546158322 32-33 3.3560993399811133E-4 34-35 2.586993241235442E-4 36-37 2.237399559987409E-4 38-39 0.0011746347689933897 40-41 0.003342115592731192 42-43 5.66341763621813E-4 44-45 4.5447178562244245E-4 46-47 0.006292686262464588 48-49 0.006649271817337581 50-51 0.00706878423483522 52-53 0.009348135036572393 54-55 0.004761465938598205 56-57 0.0 58-59 4.8943115374724574E-5 60-61 8.809760767450423E-4 62-63 8.040654668704751E-4 64-65 5.104067746221277E-4 66-67 6.013011317466163E-4 68-69 9.229273184948063E-4 70-71 0.001251545378867957 72-73 0.0026289444829852055 74-75 7.341467306208686E-4 76-77 0.0 78-79 0.0 80-81 1.1186997799937045E-4 82-83 2.0276433512385895E-4 84-85 1.6081309337409504E-4 86-87 0.0028317088181090645 88-89 0.0016570740491156748 90-91 5.803255108717343E-4 92-93 5.104067746221277E-4 94-95 3.146343131232294E-4 96-97 0.0010208135492442553 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7151159.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.1375464172689 #Duplication Level Percentage of deduplicated Percentage of total 1 89.82930581697693 68.39382941271124 2 6.2700354027029865 9.547702230224358 3 1.3710723492028511 3.1317025388659796 4 0.6576923509810922 2.003003276044225 5 0.38466912344285664 1.4643881620710322 6 0.2684953248887576 1.2265545156922484 7 0.19916768854059294 1.0614897391755684 8 0.15864240049858516 0.9662914505366392 9 0.133921972470471 0.9176841355736425 >10 0.7004027138229204 8.742631832157443 >50 0.023636913566447874 1.2466948380582468 >100 0.0026592788833123453 0.27675696530772176 >500 0.0 0.0 >1k 1.9910934814532555E-4 0.3900211106527453 >5k 9.955467407266278E-5 0.6312497929289792 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9796 0.13698478806022912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.7967494499842612E-5 0.0 6 0.0 0.0 0.0 2.7967494499842612E-5 0.0 7 0.0 0.0 0.0 2.7967494499842612E-5 0.0 8 0.0 0.0 0.0 2.7967494499842612E-5 0.0 9 0.0 0.0 0.0 2.7967494499842612E-5 0.0 10-11 6.991873624960653E-6 0.0 0.0 2.7967494499842612E-5 0.0 12-13 1.3983747249921306E-5 0.0 0.0 3.4959368124803266E-5 0.0 14-15 1.3983747249921306E-5 0.0 0.0 4.195124174976392E-5 1.3983747249921306E-5 16-17 1.3983747249921306E-5 0.0 0.0 4.195124174976392E-5 2.7967494499842612E-5 18-19 1.3983747249921306E-5 0.0 0.0 6.991873624960653E-5 2.7967494499842612E-5 20-21 1.3983747249921306E-5 0.0 0.0 6.991873624960653E-5 2.7967494499842612E-5 22-23 1.3983747249921306E-5 2.097562087488196E-5 0.0 8.390248349952783E-5 2.7967494499842612E-5 24-25 1.3983747249921306E-5 4.195124174976392E-5 0.0 8.390248349952783E-5 2.7967494499842612E-5 26-27 1.3983747249921306E-5 4.195124174976392E-5 0.0 1.1186997799937045E-4 2.7967494499842612E-5 28-29 2.7967494499842612E-5 4.195124174976392E-5 0.0 1.1886185162433111E-4 2.7967494499842612E-5 30-31 4.195124174976392E-5 4.195124174976392E-5 0.0 1.3983747249921306E-4 2.7967494499842612E-5 32-33 4.195124174976392E-5 4.894311537472457E-5 0.0 1.4682934612417372E-4 2.7967494499842612E-5 34-35 4.195124174976392E-5 6.991873624960653E-5 0.0 1.5382121974913437E-4 2.7967494499842612E-5 36-37 4.894311537472457E-5 8.390248349952783E-5 0.0 1.5382121974913437E-4 3.4959368124803266E-5 38-39 5.5934988999685224E-5 9.089435712448848E-5 0.0 1.5382121974913437E-4 4.195124174976392E-5 40-41 6.292686262464588E-5 9.788623074944915E-5 0.0 1.6780496699905567E-4 4.195124174976392E-5 42-43 6.991873624960653E-5 9.788623074944915E-5 0.0 1.7479684062401632E-4 4.195124174976392E-5 44-45 6.991873624960653E-5 9.788623074944915E-5 0.0 1.957724614988983E-4 4.195124174976392E-5 46-47 7.691060987456718E-5 9.788623074944915E-5 0.0 2.1674808237378025E-4 4.195124174976392E-5 48-49 9.788623074944913E-5 1.048781043744098E-4 0.0 2.377237032486622E-4 4.195124174976392E-5 50-51 1.3983747249921306E-4 1.1886185162433111E-4 0.0 2.517074504985835E-4 4.195124174976392E-5 52-53 1.3983747249921306E-4 1.2585372524929176E-4 0.0 2.586993241235442E-4 4.195124174976392E-5 54-55 1.6780496699905567E-4 1.2585372524929176E-4 0.0 2.7967494499842613E-4 4.195124174976392E-5 56-57 1.957724614988983E-4 1.2585372524929176E-4 0.0 2.7967494499842613E-4 4.195124174976392E-5 58-59 1.957724614988983E-4 1.2585372524929176E-4 0.0 2.9365869224834743E-4 4.195124174976392E-5 60-61 2.447155768736229E-4 1.3983747249921306E-4 0.0 3.006505658733081E-4 4.195124174976392E-5 62-63 2.586993241235442E-4 1.6081309337409502E-4 0.0 3.0764243949826873E-4 4.195124174976392E-5 64-65 2.9365869224834743E-4 1.6780496699905567E-4 0.0 3.0764243949826873E-4 4.195124174976392E-5 66-67 3.3560993399811133E-4 1.6780496699905567E-4 0.0 3.2162618674819003E-4 4.195124174976392E-5 68-69 3.775611757478753E-4 1.6780496699905567E-4 0.0 3.3560993399811133E-4 4.195124174976392E-5 70-71 4.6845553287236375E-4 1.6780496699905567E-4 0.0 3.3560993399811133E-4 4.195124174976392E-5 72-73 5.663417636218129E-4 1.7479684062401632E-4 0.0 3.3560993399811133E-4 4.195124174976392E-5 74-75 6.921954888711047E-4 1.81788714248977E-4 0.0 3.3560993399811133E-4 4.195124174976392E-5 76-77 7.970735932455145E-4 1.81788714248977E-4 0.0 3.3560993399811133E-4 4.894311537472457E-5 78-79 9.718704338695308E-4 1.81788714248977E-4 0.0 3.495936812480327E-4 5.5934988999685224E-5 80-81 0.0013564234832423667 1.8878058787393765E-4 0.0 3.63577428497954E-4 5.5934988999685224E-5 82-83 0.0017689440271150452 1.957724614988983E-4 0.0 3.775611757478753E-4 5.5934988999685224E-5 84-85 0.0022443914336123696 1.957724614988983E-4 0.0 4.1252054387267854E-4 5.5934988999685224E-5 86-87 0.0029156113016085925 2.1674808237378025E-4 0.0 4.2650429112259984E-4 5.5934988999685224E-5 88-89 0.004055286702477179 2.237399559987409E-4 0.0 4.4048803837252115E-4 5.5934988999685224E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 330 0.0 25.90719 3 CTCGCGT 185 0.0 21.826235 52-53 CGCGTAG 185 0.0 21.824251 54-55 TCGCGTA 195 0.0 20.70694 52-53 ACTCGCG 205 0.0 19.697536 50-51 CGTAGCG 215 0.0 18.777561 56-57 CGTACGC 230 0.0 18.585464 20-21 GCGACGA 255 3.6379788E-12 15.832616 60-61 CAACGCA 5700 0.0 15.623528 12-13 TCAACGC 5425 0.0 15.32119 10-11 ATCAACG 5855 0.0 15.128858 10-11 CGACGAC 305 0.0 14.79441 60-61 GATCGGA 620 0.0 14.576381 1 CGCTAGT 280 1.6370905E-11 14.418788 90-91 ACGCAGA 6290 0.0 14.195998 14-15 AACGCAG 6030 0.0 13.78394 12-13 ACGTACG 295 4.0017767E-11 13.685343 20-21 CGCAGAG 6360 0.0 13.292957 14-15 GTATCAA 7835 0.0 12.790843 9 AGCGACG 320 1.5279511E-10 12.616483 58-59 >>END_MODULE