FastQCFastQC Report
Sat 18 Jun 2016
SRR578582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7195919
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83939411.664861708421121No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT214090.29751585586219076No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG92290.12825325021029282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG90480.1257379356271242No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT75400.10478161302260351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1878700.090.950145
AAGCAGT1889650.090.906651
TGGTATC1883050.090.877567
GTGGTAT1884850.090.8638846
GGTATCA1893750.090.61818
GTATCAA1895450.090.614539
GCAGTGG1902000.090.0439763
AGCAGTG1909900.089.9054262
CAGTGGT1912650.089.380484
TCAACGC1884300.046.0882512-13
CAACGCA1884000.046.02372412-13
ATCAACG1884200.046.00894510-11
ACGCAGA1889800.045.9206614-15
CGCAGAG1890050.045.90616616-17
AACGCAG1893000.045.89196814-15
AGAGTAC1837000.045.68666518-19
TATCAAC1899400.045.57322710-11
CAGAGTA1887500.045.3703218-19
GAGTACT1115550.045.3360220-21
GCAGAGT1913250.045.1099216-17
Produced by FastQC (version 0.11.5)