##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578582_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7195919 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.60993196282504 31.0 31.0 34.0 27.0 34.0 2 30.903967095794158 31.0 31.0 34.0 27.0 34.0 3 30.993770357893133 31.0 31.0 34.0 27.0 34.0 4 34.438756467381026 37.0 35.0 37.0 30.0 37.0 5 34.49403432695671 37.0 35.0 37.0 30.0 37.0 6 34.48241718674154 37.0 35.0 37.0 30.0 37.0 7 34.407137017523404 37.0 35.0 37.0 30.0 37.0 8 34.359165382489714 37.0 35.0 37.0 30.0 37.0 9 35.791406212326734 39.0 35.0 39.0 30.0 39.0 10-11 35.776665704547256 39.0 35.0 39.0 30.0 39.0 12-13 35.8309475829286 39.0 35.0 39.0 30.0 39.0 14-15 36.858602007610145 40.0 36.0 41.0 31.0 41.0 16-17 36.82135304747038 40.0 36.0 41.0 30.5 41.0 18-19 36.82277128188908 40.0 36.0 41.0 30.0 41.0 20-21 36.724148437468514 39.5 36.0 41.0 30.0 41.0 22-23 36.42127308548081 39.0 36.0 40.5 29.5 41.0 24-25 36.30592332959834 39.0 36.0 40.0 29.0 41.0 26-27 36.2635108872126 39.0 36.0 40.5 29.0 41.0 28-29 35.94587397384545 39.0 35.0 41.0 27.0 41.0 30-31 35.65778693451108 39.0 34.5 40.5 26.5 41.0 32-33 35.37666662173379 38.0 34.0 40.0 25.5 41.0 34-35 35.183529581141755 38.0 34.0 40.0 25.0 41.0 36-37 35.190631940131624 38.0 34.0 40.0 25.0 41.0 38-39 34.531148766404954 37.0 33.5 40.0 25.0 41.0 40-41 34.48790307395066 37.5 33.0 40.0 25.0 41.0 42-43 34.29883174060186 37.5 33.0 40.0 24.0 41.0 44-45 34.135606237368705 37.0 33.0 40.0 23.0 41.0 46-47 33.924398537559966 37.0 33.0 40.0 23.0 41.0 48-49 33.5481757090373 37.0 33.0 40.0 21.0 41.0 50-51 32.24711847089996 36.0 31.5 39.0 14.5 40.5 52-53 31.818476416980236 36.5 31.0 39.0 5.0 40.0 54-55 31.455286392189794 37.0 30.5 40.0 2.0 41.0 56-57 30.91985318345023 37.0 29.5 40.0 2.0 41.0 58-59 30.57719118294689 37.0 28.5 40.0 2.0 41.0 60-61 30.276416257603792 36.0 28.0 40.0 2.0 41.0 62-63 29.939094367237875 36.0 28.0 39.5 2.0 41.0 64-65 29.622982484933473 35.0 28.0 39.0 2.0 41.0 66-67 29.337373516850313 35.0 27.0 39.0 2.0 41.0 68-69 29.004139221133535 35.0 26.0 39.0 2.0 40.0 70-71 28.615419934549013 35.0 26.0 38.0 2.0 40.0 72-73 28.224029342186867 35.0 26.0 37.5 2.0 40.0 74-75 27.830140806198624 34.0 25.0 37.0 2.0 39.0 76-77 27.433955482267102 34.0 24.5 36.0 2.0 39.0 78-79 27.042168762600024 34.0 23.5 36.0 2.0 39.0 80-81 26.64594946107648 34.0 22.0 35.5 2.0 37.5 82-83 26.27024435655821 33.5 20.5 35.0 2.0 37.0 84-85 25.846590755121063 33.0 19.5 35.0 2.0 36.5 86-87 25.461279233409936 33.0 18.0 35.0 2.0 36.0 88-89 25.107795682525055 33.0 14.5 35.0 2.0 36.0 90-91 24.73496630520716 32.0 7.5 35.0 2.0 35.5 92-93 24.367187776849626 32.0 4.0 35.0 2.0 35.0 94-95 23.924368937449128 32.0 2.0 35.0 2.0 35.0 96-97 23.472724818053123 32.0 2.0 35.0 2.0 35.0 98-99 22.92537478534708 31.0 2.0 34.5 2.0 35.0 100-101 21.398969193510936 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5110284349461907 1101 2 0.549485246242071 1101 3 0.5542445341855142 1101 4 0.7012043926492524 1101 5 0.6213917975858223 1101 6 0.5155471488905334 1101 7 0.5108005503098312 1101 8 0.5694084062764944 1101 9 0.528659416109079 1101 10-11 0.6646561447607269 1101 12-13 0.5310422148164164 1101 14-15 0.7291006456654898 1101 16-17 0.6717260078384584 1101 18-19 0.6653453395467537 1101 20-21 0.734879758410294 1101 22-23 0.7908198119283085 1101 24-25 0.9299141882538322 1101 26-27 0.8487215923285518 1101 28-29 0.7624939393416739 1101 30-31 0.8882535063705248 1101 32-33 1.0402110641939757 1101 34-35 1.036429846547378 1101 36-37 0.8720066628097598 1101 38-39 1.0110003158832725 1101 40-41 0.9026996892995243 1101 42-43 1.1987598660336758 1101 44-45 1.076058053844399 1101 46-47 1.0433934261477233 1101 48-49 1.1655889731633806 1101 50-51 1.1429664299388378 1101 52-53 1.1678141243230264 1101 54-55 1.0708766819037692 1101 56-57 0.982866492286739 1101 58-59 1.0299797339648045 1101 60-61 1.147451075174466 1101 62-63 1.1011293179466008 1101 64-65 1.1736954005558182 1101 66-67 1.0938299349984746 1101 68-69 1.188653555433003 1101 70-71 1.06790942424713 1101 72-73 1.1731182630595036 1101 74-75 1.1943388802468355 1101 76-77 1.1402962955500904 1101 78-79 1.1875448247161238 1101 80-81 1.2290500364754458 1101 82-83 1.2956603055573659 1101 84-85 1.4160749362705047 1101 86-87 1.440860802117733 1101 88-89 1.5893579211604028 1101 90-91 1.7021968931478497 1101 92-93 1.628904485954461 1101 94-95 1.6975104865935648 1101 96-97 1.968004299990664 1101 98-99 2.1177462426063265 1101 100-101 2.3892833593984193 1102 1 0.41400750596881153 1102 2 0.4324002518612886 1102 3 0.3850025274236373 1102 4 0.4772955027615282 1102 5 0.4108925801313532 1102 6 0.34973697321171215 1102 7 0.4604670645045701 1102 8 0.5543277404425453 1102 9 0.48228765891808223 1102 10-11 0.5203149308039059 1102 12-13 0.5360714551798935 1102 14-15 0.6795187559650131 1102 16-17 0.668809164545408 1102 18-19 0.552610838622364 1102 20-21 0.6154894406033264 1102 22-23 0.6065261963327799 1102 24-25 0.7286827575418897 1102 26-27 0.7317682133898487 1102 28-29 0.7073379193265055 1102 30-31 0.8150634883237657 1102 32-33 0.8057035714381087 1102 34-35 0.8522068294492584 1102 36-37 0.7531345664520117 1102 38-39 0.8010800979728501 1102 40-41 0.7819956974354128 1102 42-43 1.0096325821516103 1102 44-45 0.9871416394521546 1102 46-47 1.0154548677306323 1102 48-49 1.1706979801830784 1102 50-51 1.0289089673735887 1102 52-53 0.9811242110762244 1102 54-55 0.9397194915979199 1102 56-57 0.8591135823609051 1102 58-59 0.9429800159545856 1102 60-61 1.0154354034655455 1102 62-63 1.0513204198571344 1102 64-65 0.9650970235293777 1102 66-67 0.9945565235262173 1102 68-69 1.1550403201099186 1102 70-71 1.0919100104213584 1102 72-73 1.1136900188394492 1102 74-75 1.2902647470659367 1102 76-77 1.133950542491121 1102 78-79 1.0925961292918416 1102 80-81 1.2194370841710374 1102 82-83 1.415396395237316 1102 84-85 1.412178744374586 1102 86-87 1.4076008991833646 1102 88-89 1.5577086193662204 1102 90-91 1.5068045047700913 1102 92-93 1.4248561154835322 1102 94-95 1.4025431296484427 1102 96-97 1.7019780552449966 1102 98-99 1.8257328701026019 1102 100-101 2.084627013224619 1103 1 0.29368095278782747 1103 2 0.2856445814966122 1103 3 0.1698865827592897 1103 4 0.29968654934759087 1103 5 0.2960855573660126 1103 6 0.17419521309120967 1103 7 0.388862917416148 1103 8 0.30400175045602396 1103 9 0.5130881237632181 1103 10-11 0.4340393058303036 1103 12-13 0.26794335255507207 1103 14-15 0.53107042224174 1103 16-17 0.5136438006903248 1103 18-19 0.49349542577236605 1103 20-21 0.6138802734451119 1103 22-23 0.6434525149942019 1103 24-25 0.5403244195976526 1103 26-27 0.6768787998430454 1103 28-29 0.6626849396083969 1103 30-31 0.6147995133871191 1103 32-33 0.8164223247801985 1103 34-35 0.7343813989125536 1103 36-37 0.766934600138832 1103 38-39 0.5564235645875542 1103 40-41 0.6329328582642759 1103 42-43 0.45927210024887444 1103 44-45 0.5514780596952775 1103 46-47 0.6205317887105579 1103 48-49 0.637204170753698 1103 50-51 0.5640076095357003 1103 52-53 0.5581413160110351 1103 54-55 0.6813434624574057 1103 56-57 0.601583889580489 1103 58-59 0.7459504117724514 1103 60-61 0.7181057709139829 1103 62-63 0.7117549378660257 1103 64-65 0.8025318966803106 1103 66-67 0.6880750090363925 1103 68-69 0.8122088251222017 1103 70-71 0.794232154059312 1103 72-73 0.8085601148910655 1103 74-75 0.9444097141445233 1103 76-77 0.8635267015857657 1103 78-79 0.9410716079397297 1103 80-81 0.8373726072423899 1103 82-83 1.0183472664811788 1103 84-85 0.8961933832366213 1103 86-87 1.1694960698956933 1103 88-89 1.2512165730650473 1103 90-91 1.029933972692124 1103 92-93 1.1042123291661028 1103 94-95 1.1309113508316884 1103 96-97 1.293837646596483 1103 98-99 1.3898814121928496 1103 100-101 1.6963293984280625 1104 1 0.14783745074246468 1104 2 0.22666436252471556 1104 3 0.21782343463833698 1104 4 0.24761139929057663 1104 5 0.10047129886180528 1104 6 0.10504572307141302 1104 7 0.3306341866187026 1104 8 0.19169339680227893 1104 9 0.31520033326130914 1104 10-11 0.37783708662420423 1104 12-13 0.21920886593241562 1104 14-15 0.3773123506480758 1104 16-17 0.43498644947532483 1104 18-19 0.6715423972376513 1104 20-21 0.5268644414623012 1104 22-23 0.40411146102297124 1104 24-25 0.43386339132777607 1104 26-27 0.477667396676722 1104 28-29 0.6132801200654541 1104 30-31 0.5881112106665043 1104 32-33 0.754628149452472 1104 34-35 0.6999641610844876 1104 36-37 0.7524059411779973 1104 38-39 0.5616950923494315 1104 40-41 0.540309283895219 1104 42-43 0.7026124111354761 1104 44-45 0.9381423592729305 1104 46-47 0.7512092021168755 1104 48-49 0.816668916488382 1104 50-51 0.7562718349288531 1104 52-53 0.6720549559561668 1104 54-55 0.7961204707145271 1104 56-57 0.7404293368728148 1104 58-59 0.919030871264308 1104 60-61 0.9104208094856965 1104 62-63 0.7229356873876647 1104 64-65 0.9082941731077945 1104 66-67 0.6512223388535325 1104 68-69 0.7920920928046051 1104 70-71 0.9016352358735809 1104 72-73 0.7038080591402505 1104 74-75 0.7749578898137877 1104 76-77 0.9303278024535082 1104 78-79 0.9873420245381581 1104 80-81 0.898018350047753 1104 82-83 0.9342048627806747 1104 84-85 0.8765398848180936 1104 86-87 0.9908666837021158 1104 88-89 1.0993990271386842 1104 90-91 1.1680191636884238 1104 92-93 1.1351071336548522 1104 94-95 1.3017126729258806 1104 96-97 1.5018298677961006 1104 98-99 1.6399635609244 1104 100-101 1.9518980306171372 1105 1 -0.03540565070484192 1105 2 0.19133761942939032 1105 3 0.22012466763744953 1105 4 0.0973042344096342 1105 5 0.15637575227967915 1105 6 0.20418331357684139 1105 7 0.3153902740024037 1105 8 0.35927726488773004 1105 9 0.28757719191205666 1105 10-11 0.2903037190934441 1105 12-13 0.2844282710084016 1105 14-15 0.07595750928224021 1105 16-17 0.3465204184283621 1105 18-19 0.18057284166701493 1105 20-21 0.18037921497479203 1105 22-23 0.386095011205839 1105 24-25 0.5466285373214959 1105 26-27 0.5553887072765491 1105 28-29 0.7158642051302664 1105 30-31 0.5928530441754631 1105 32-33 0.6208401377289121 1105 34-35 0.459026943783563 1105 36-37 0.7758319228426842 1105 38-39 0.6180707108796923 1105 40-41 0.5551229932074193 1105 42-43 0.8454540256621854 1105 44-45 0.8611832582128542 1105 46-47 0.6224649868422034 1105 48-49 0.6807277323596992 1105 50-51 0.7188274567527486 1105 52-53 0.6391340410376607 1105 54-55 0.8957576005560881 1105 56-57 1.0098559438831387 1105 58-59 0.9283470051817453 1105 60-61 0.8449061873023922 1105 62-63 0.9345356264109022 1105 64-65 1.0437080000339307 1105 66-67 0.8913257851533665 1105 68-69 0.9461768543999263 1105 70-71 0.940207767622109 1105 72-73 0.829511016624533 1105 74-75 0.8359362525209306 1105 76-77 0.7578207283700813 1105 78-79 0.900301937323583 1105 80-81 0.9400964893217996 1105 82-83 1.0902057190146017 1105 84-85 1.2090702090742695 1105 86-87 1.199630313065768 1105 88-89 1.084169927536255 1105 90-91 1.2880388615196345 1105 92-93 1.3548417122642498 1105 94-95 1.3986070306836709 1105 96-97 1.577182417526103 1105 98-99 1.8366918599286315 1105 100-101 2.1719901383546194 1106 1 0.23784258448007023 1106 2 0.27492880068533765 1106 3 0.21728255367089844 1106 4 0.10365955126502513 1106 5 0.2838481202835368 1106 6 0.22167455130180258 1106 7 0.330053712211658 1106 8 0.3575369586760999 1106 9 0.33821626083397405 1106 10-11 0.23462900947782828 1106 12-13 0.4069608065402832 1106 14-15 0.2978541046473353 1106 16-17 0.3960272547847197 1106 18-19 0.3730059570873365 1106 20-21 0.31504036005479463 1106 22-23 0.40302273847671444 1106 24-25 0.5366677131387334 1106 26-27 0.3859540724468147 1106 28-29 0.4939800138618722 1106 30-31 0.5876099407827979 1106 32-33 0.5445857551828368 1106 34-35 0.47997818499610645 1106 36-37 0.5586421143513789 1106 38-39 0.5501838379318755 1106 40-41 0.47703866746417845 1106 42-43 0.6142252798926151 1106 44-45 0.5521727533426954 1106 46-47 0.6665111155033756 1106 48-49 0.6979032070325246 1106 50-51 0.5535113693805798 1106 52-53 0.44770226811210634 1106 54-55 0.4995793705363525 1106 56-57 0.3457524383956141 1106 58-59 0.48581949889923237 1106 60-61 0.37849214105471063 1106 62-63 0.43340771635171293 1106 64-65 0.5855568340805313 1106 66-67 0.5960600142203596 1106 68-69 0.46022976279349237 1106 70-71 0.5564185803603579 1106 72-73 0.5277035856744199 1106 74-75 0.4336554710286826 1106 76-77 0.48255178748168603 1106 78-79 0.5792188834360346 1106 80-81 0.6484157136187143 1106 82-83 0.794872240553044 1106 84-85 0.8372221877645067 1106 86-87 0.8734970135189997 1106 88-89 0.9133304517247574 1106 90-91 0.7641250747983968 1106 92-93 0.8999332976349343 1106 94-95 0.9268372669872562 1106 96-97 1.0834468561059474 1106 98-99 1.2805295082090886 1106 100-101 1.5496144807526235 1107 1 0.09417013382869399 1107 2 0.11975820096736811 1107 3 0.14718061109654101 1107 4 0.12234452932574413 1107 5 0.23149581635762218 1107 6 0.1694565092782767 1107 7 0.15217438954404372 1107 8 0.1433255115528027 1107 9 0.09236555743208186 1107 10-11 0.13342812889464994 1107 12-13 0.20040899400807177 1107 14-15 0.18480357011908666 1107 16-17 0.16089859100330983 1107 18-19 0.1968504285021524 1107 20-21 0.25289939525966076 1107 22-23 0.3852579957980211 1107 24-25 0.44228809369991495 1107 26-27 0.3734334299829669 1107 28-29 0.47199598072177196 1107 30-31 0.48413924804967934 1107 32-33 0.519393377899739 1107 34-35 0.4765006577109929 1107 36-37 0.3730316450184006 1107 38-39 0.49128096553918255 1107 40-41 0.41313190185804416 1107 42-43 0.3591711208829338 1107 44-45 0.4483074732416199 1107 46-47 0.4707174239597052 1107 48-49 0.5135765282173921 1107 50-51 0.4416013062549311 1107 52-53 0.44516182401302373 1107 54-55 0.5981792347406198 1107 56-57 0.5017084650372858 1107 58-59 0.5235641928646864 1107 60-61 0.5694166437373802 1107 62-63 0.6611150612863277 1107 64-65 0.6836049457175868 1107 66-67 0.7589485738570012 1107 68-69 0.7187525393587855 1107 70-71 0.7988628174258601 1107 72-73 0.7884647083829286 1107 74-75 0.781199042121834 1107 76-77 0.795022811340953 1107 78-79 0.8438494574218041 1107 80-81 0.9120575331926482 1107 82-83 0.960018466651892 1107 84-85 1.0921501877806072 1107 86-87 1.0105925135347533 1107 88-89 1.0850617226925507 1107 90-91 1.0912429912424528 1107 92-93 1.164416501051047 1107 94-95 1.1863149201710996 1107 96-97 1.2772857946394645 1107 98-99 1.3924934114333603 1107 100-101 1.636933629727185 1108 1 0.1962698368936202 1108 2 0.22690774583715267 1108 3 0.004804680590535071 1108 4 0.21527566276170518 1108 5 0.3060636676031194 1108 6 0.29249518136321484 1108 7 0.2747330652281974 1108 8 0.24714031258684344 1108 9 0.2246734190374795 1108 10-11 0.3563624420407763 1108 12-13 0.3401595357139513 1108 14-15 0.2573909422315026 1108 16-17 0.3571927999181739 1108 18-19 0.14667986106675812 1108 20-21 0.2149194900551521 1108 22-23 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96-97 -4.438586084309744 2316 98-99 -4.593925830981753 2316 100-101 -4.824578723472403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80001.0 3 13100.0 4 11191.0 5 10650.0 6 12212.0 7 14894.0 8 18625.0 9 21868.0 10 25607.0 11 30933.0 12 32937.0 13 37484.0 14 43394.0 15 52172.0 16 66639.0 17 90476.0 18 130589.0 19 190805.0 20 245416.0 21 175863.0 22 101985.0 23 89584.0 24 92546.0 25 98806.0 26 108069.0 27 121747.0 28 141374.0 29 165211.0 30 194252.0 31 231555.0 32 281415.0 33 348857.0 34 444734.0 35 602514.0 36 849270.0 37 1022760.0 38 842884.0 39 153326.0 40 174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.653850590137527 47.64205869220977 24.65781289936322 17.046277818289486 2 16.332251262022357 48.83438744162895 19.59369877794667 15.239662518402028 3 42.401851539620246 21.554433005638533 20.120023994226248 15.923691460514974 4 16.291180536294565 20.34534022843526 21.16817896923438 42.1953002660358 5 16.08590503724575 46.49943357970947 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37.78817601871099 18.71446310516912 52-53 19.060147454184552 24.964327955079433 36.842642730236754 19.13288186049926 54-55 19.99797568964542 25.509897501214102 35.2475671779765 19.24455963116398 56-57 20.226198003822844 26.10924529109851 33.84703167778651 19.817525027292135 58-59 20.581082476143933 26.7678899219692 33.267835523912375 19.38319207797449 60-61 20.606076784148083 26.54518603975895 32.86683059745759 19.98190657863537 62-63 20.02458136663166 26.81395510838173 33.27020329300491 19.891260231981693 64-65 20.308505950983136 26.683544342516573 33.05367473538866 19.954274971111634 66-67 19.989871661367307 26.260800896465717 33.05105600269162 20.698271439475356 68-69 20.165502289710407 26.5901840318506 33.14334091820036 20.10097276023863 70-71 19.739330801212276 26.583412787987637 33.40155020867927 20.275706202120823 72-73 19.901103848883377 26.283786616048655 33.39625827393106 20.418851261136904 74-75 20.154197447727547 26.093997641859477 33.10953174136255 20.642273169050423 76-77 20.35837984582234 26.34537029789635 33.084271913120645 20.211977943160665 78-79 20.52547354488527 26.698108879175148 32.196700479384255 20.57971709655533 80-81 20.299333665489105 26.559774795883744 32.357491930554275 20.783399608072873 82-83 20.89393162980142 26.669432266936777 31.893504277829386 20.543131825432422 84-85 21.040557438058443 26.82479829937262 32.00805794501686 20.12658631755208 86-87 20.905614672766045 27.21647132334447 31.628954330999004 20.248959672890486 88-89 20.84443480793079 26.899255000017032 31.30710726327191 20.94920292878027 90-91 21.093027164754595 27.208256352556365 31.47711472549057 20.22160175719847 92-93 21.071702348973602 27.450148157642957 31.27358017098257 20.20456932240087 94-95 21.02320676572039 27.336498102945612 31.084539528106852 20.555755603227148 96-97 20.52674716461386 26.87119081978097 31.791272319538884 20.81078969606629 98-99 20.48317045405417 27.18665102121075 31.34599112068015 20.98418740405493 100-101 21.195115016403214 26.577056601182385 30.933848583912294 21.293979798502104 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6391.0 1 3882.5 2 1072.5 3 718.0 4 598.0 5 503.5 6 601.0 7 736.0 8 717.5 9 757.5 10 1058.5 11 5427.5 12 92734.5 13 104683.5 14 30348.5 15 28474.0 16 31686.5 17 35822.0 18 40476.0 19 44722.0 20 48787.5 21 53599.5 22 58847.5 23 64929.5 24 71431.5 25 79062.0 26 90522.0 27 102832.5 28 112944.0 29 121502.0 30 128819.5 31 138412.0 32 151262.0 33 164382.0 34 180676.5 35 191230.0 36 196963.5 37 204504.5 38 208168.5 39 212568.5 40 218058.0 41 223941.0 42 226728.0 43 225261.0 44 226478.5 45 227816.5 46 225485.5 47 225404.5 48 222189.0 49 214066.0 50 207068.0 51 196329.5 52 185797.0 53 177134.0 54 169798.0 55 164836.5 56 157998.0 57 142743.0 58 138825.5 59 117679.5 60 76349.0 61 54968.0 62 40041.0 63 28884.5 64 21350.5 65 15566.0 66 10817.0 67 7654.5 68 5420.5 69 3991.0 70 3118.5 71 2634.0 72 2243.5 73 1991.5 74 1837.0 75 1699.0 76 1600.0 77 1477.0 78 1409.0 79 1312.0 80 1216.5 81 1115.0 82 995.0 83 909.5 84 814.5 85 708.0 86 586.0 87 477.0 88 374.0 89 289.5 90 233.0 91 180.5 92 119.5 93 74.5 94 56.5 95 41.0 96 21.5 97 15.5 98 17.5 99 10.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12084627411731567 2 0.1091313006719503 3 0.1045453680065048 4 0.09256635601373502 5 0.0960405474269513 6 0.08752183008174495 7 0.09676317924090029 8 0.09338626518725406 9 0.08804990717655382 10-11 0.0788988869941421 12-13 0.07085960806395958 14-15 0.07871128065782841 16-17 0.06928232516235938 18-19 0.061889245835035106 20-21 0.057845287030051346 22-23 0.05012563370988472 24-25 0.04755473206410467 26-27 0.04469894672244087 28-29 0.04685294539863498 30-31 0.03845929894430441 32-33 0.05036187872598343 34-35 0.060958162536293145 36-37 0.051313807173204704 38-39 0.0531690253878622 40-41 0.05346085746657238 42-43 0.04895135701221762 44-45 0.04835379608914442 46-47 0.04818703490131004 48-49 0.04825651872957436 50-51 0.04961145338072872 52-53 0.046026087842289495 54-55 0.04649857787448691 56-57 0.04684599701580854 58-59 0.04956281470094369 60-61 0.045157539988985425 62-63 0.05400283132703411 64-65 0.04972262750595164 66-67 0.04487265629310169 68-69 0.042913212336047696 70-71 0.05088300743796589 72-73 0.04478232731635806 74-75 0.04707529364908082 76-77 0.04235734170993309 78-79 0.04667923582797416 80-81 0.048784595824383235 82-83 0.03870249234322955 84-85 0.0488401828869947 86-87 0.05155700057212985 88-89 0.04681125510167638 90-91 0.043163354117799264 92-93 0.04037010422157337 94-95 0.04431678566698708 96-97 0.031656832157226894 98-99 0.03265739928423319 100-101 0.024312391509687642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7195919.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.37137504719006 #Duplication Level Percentage of deduplicated Percentage of total 1 91.26019953555841 51.444629349003634 2 5.297392355309254 5.972425824665109 3 1.3110642886096415 2.2171949017257457 4 0.5982131564012857 1.3488839279064095 5 0.38118969585855916 1.0744093654683573 6 0.24969303239938248 0.8445323745633461 7 0.17810103033036487 0.7027859983930778 8 0.1395480061241843 0.6293210392251175 9 0.11108774159656724 0.5635951870216885 >10 0.44462883603979475 3.795237809681942 >50 0.011158269864808331 0.4656937583082124 >100 0.011302259356906446 1.3760132322903535 >500 0.003359091488183271 1.3662079129844231 >1k 0.002667513828751051 2.9572767956274117 >5k 2.9639042541678345E-4 1.2713903771029929 >10k+ 9.879680847226116E-5 23.9704021460321 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 839394 11.664861708421121 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 21409 0.29751585586219076 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9229 0.12825325021029282 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG 9048 0.1257379356271242 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 7540 0.10478161302260351 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.169029695859556E-5 0.0 2 0.0 1.3896765652865186E-5 0.0 5.558706261146074E-5 0.0 3 0.0 1.3896765652865186E-5 0.0 5.558706261146074E-5 1.3896765652865186E-5 4 0.0 1.3896765652865186E-5 0.0 5.558706261146074E-5 1.3896765652865186E-5 5 0.0 1.3896765652865186E-5 0.0 5.558706261146074E-5 1.3896765652865186E-5 6 0.0 2.779353130573037E-5 0.0 5.558706261146074E-5 1.3896765652865186E-5 7 0.0 2.779353130573037E-5 0.0 5.558706261146074E-5 1.3896765652865186E-5 8 0.0 2.779353130573037E-5 0.0 6.948382826432594E-5 1.3896765652865186E-5 9 0.0 2.779353130573037E-5 0.0 6.948382826432594E-5 2.779353130573037E-5 10-11 0.0 2.779353130573037E-5 0.0 6.948382826432594E-5 2.779353130573037E-5 12-13 0.0 2.779353130573037E-5 0.0 6.948382826432594E-5 4.169029695859556E-5 14-15 0.0 4.169029695859556E-5 0.0 6.948382826432594E-5 4.169029695859556E-5 16-17 0.0 4.169029695859556E-5 0.0 6.948382826432594E-5 4.169029695859556E-5 18-19 0.0 5.558706261146074E-5 0.0 8.338059391719112E-5 4.169029695859556E-5 20-21 1.3896765652865186E-5 6.253544543789334E-5 0.0 9.72773595700563E-5 4.169029695859556E-5 22-23 1.3896765652865186E-5 6.948382826432594E-5 0.0 1.042257423964889E-4 4.169029695859556E-5 24-25 1.3896765652865186E-5 6.948382826432594E-5 0.0 1.1117412522292149E-4 5.558706261146074E-5 26-27 1.3896765652865186E-5 8.338059391719112E-5 0.0 1.1117412522292149E-4 5.558706261146074E-5 28-29 1.3896765652865186E-5 9.72773595700563E-5 0.0 1.1117412522292149E-4 5.558706261146074E-5 30-31 1.3896765652865186E-5 1.1117412522292149E-4 0.0 1.1812250804935408E-4 5.558706261146074E-5 32-33 1.3896765652865186E-5 1.2507089087578668E-4 0.0 1.2507089087578668E-4 5.558706261146074E-5 34-35 2.779353130573037E-5 1.2507089087578668E-4 0.0 1.4591603935508446E-4 5.558706261146074E-5 36-37 2.779353130573037E-5 1.2507089087578668E-4 0.0 1.5286442218151705E-4 5.558706261146074E-5 38-39 2.779353130573037E-5 1.2507089087578668E-4 0.0 1.5981280500794963E-4 5.558706261146074E-5 40-41 2.779353130573037E-5 1.2507089087578668E-4 0.0 1.7370957066081485E-4 5.558706261146074E-5 42-43 3.474191413216296E-5 1.3896765652865188E-4 0.0 1.8065795348724744E-4 5.558706261146074E-5 44-45 4.169029695859556E-5 1.3896765652865188E-4 0.0 1.945547191401126E-4 5.558706261146074E-5 46-47 6.253544543789334E-5 1.3896765652865188E-4 0.0 2.015031019665452E-4 5.558706261146074E-5 48-49 6.948382826432594E-5 1.3896765652865188E-4 0.0 2.084514847929778E-4 5.558706261146074E-5 50-51 9.03289767436237E-5 1.3896765652865188E-4 0.0 2.153998676194104E-4 5.558706261146074E-5 52-53 9.72773595700563E-5 1.3896765652865188E-4 0.0 2.2234825044584297E-4 5.558706261146074E-5 54-55 9.72773595700563E-5 1.4591603935508446E-4 0.0 2.2234825044584297E-4 6.948382826432594E-5 56-57 1.2507089087578668E-4 1.5286442218151705E-4 0.0 2.3624501609870817E-4 6.948382826432594E-5 58-59 1.3896765652865188E-4 1.5286442218151705E-4 0.0 2.3624501609870817E-4 6.948382826432594E-5 60-61 1.6676118783438224E-4 1.6676118783438224E-4 0.0 2.570901645780059E-4 6.948382826432594E-5 62-63 2.2234825044584297E-4 1.6676118783438224E-4 0.0 2.6403854740443853E-4 6.948382826432594E-5 64-65 2.8488369588373637E-4 1.6676118783438224E-4 0.0 2.6403854740443853E-4 8.338059391719112E-5 66-67 3.265739928423319E-4 1.6676118783438224E-4 0.0 2.7793531305730375E-4 9.03289767436237E-5 68-69 3.891094382802252E-4 1.6676118783438224E-4 0.0 2.918320787101689E-4 9.72773595700563E-5 70-71 5.836641574203378E-4 1.6676118783438224E-4 0.0 2.918320787101689E-4 9.72773595700563E-5 72-73 7.226318139489896E-4 1.6676118783438224E-4 0.0 2.918320787101689E-4 9.72773595700563E-5 74-75 8.754962361305068E-4 1.6676118783438224E-4 0.0 2.918320787101689E-4 9.72773595700563E-5 76-77 0.0010214122754855911 1.6676118783438224E-4 0.0 2.918320787101689E-4 9.72773595700563E-5 78-79 0.0012020702289728387 1.6676118783438224E-4 0.0 2.918320787101689E-4 9.72773595700563E-5 80-81 0.0015216958389887379 1.6676118783438224E-4 0.0 2.918320787101689E-4 9.72773595700563E-5 82-83 0.001862166597483935 1.6676118783438224E-4 0.0 3.057288443630341E-4 9.72773595700563E-5 84-85 0.0023207598640284863 1.6676118783438224E-4 0.0 3.057288443630341E-4 9.72773595700563E-5 86-87 0.0030155981466717455 1.6676118783438224E-4 0.0 3.057288443630341E-4 9.72773595700563E-5 88-89 0.003981423359545876 1.6676118783438224E-4 0.0 3.196256100158993E-4 9.72773595700563E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 187870 0.0 90.95014 5 AAGCAGT 188965 0.0 90.90665 1 TGGTATC 188305 0.0 90.87756 7 GTGGTAT 188485 0.0 90.863884 6 GGTATCA 189375 0.0 90.6181 8 GTATCAA 189545 0.0 90.61453 9 GCAGTGG 190200 0.0 90.043976 3 AGCAGTG 190990 0.0 89.905426 2 CAGTGGT 191265 0.0 89.38048 4 TCAACGC 188430 0.0 46.08825 12-13 CAACGCA 188400 0.0 46.023724 12-13 ATCAACG 188420 0.0 46.008945 10-11 ACGCAGA 188980 0.0 45.92066 14-15 CGCAGAG 189005 0.0 45.906166 16-17 AACGCAG 189300 0.0 45.891968 14-15 AGAGTAC 183700 0.0 45.686665 18-19 TATCAAC 189940 0.0 45.573227 10-11 CAGAGTA 188750 0.0 45.37032 18-19 GAGTACT 111555 0.0 45.33602 20-21 GCAGAGT 191325 0.0 45.10992 16-17 >>END_MODULE