FastQCFastQC Report
Sat 18 Jun 2016
SRR578582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7195919
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA646230.898050686785107No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99260.13793929587033985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3800.031.2340183
CGCGTAG2000.029.67458554-55
CTCGCGT2150.026.50267452-53
ACTCGCG2200.025.9014250-51
TCGCGTA2350.025.25742352-53
CGTAGCG2800.022.89039256-57
CGTACGC2900.020.4638120-21
GCGACGA3100.019.90972160-61
AGCGACG3650.016.90968558-59
CGCTAGT3700.016.68169890-91
CAACGCA52000.016.47931912-13
ACGTACG3850.016.03086920-21
TCAACGC50700.016.01229310-11
ATCAACG53650.015.97249910-11
GTCACGT8800.015.1059074
CGACGAC4000.014.83657160-61
ACGCAGA59050.014.55204814-15
ACGTCAC9600.014.34161952
AACGCAG57550.014.18889212-13
GTGTACG9900.013.9068339
Produced by FastQC (version 0.11.5)