##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578581_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6814517
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03354617796096	31.0	31.0	34.0	28.0	34.0
2	31.386668783715706	33.0	31.0	34.0	28.0	34.0
3	31.507348943439425	34.0	31.0	34.0	28.0	34.0
4	34.89086357844584	37.0	35.0	37.0	32.0	37.0
5	34.93325205586838	37.0	35.0	37.0	32.0	37.0
6	34.97028828308741	37.0	35.0	37.0	32.0	37.0
7	34.915336626205494	37.0	35.0	37.0	32.0	37.0
8	34.94985132475273	37.0	35.0	37.0	32.0	37.0
9	36.458279581663675	39.0	37.0	39.0	32.0	39.0
10-11	36.352134494638435	39.0	37.0	39.0	32.0	39.0
12-13	36.40715460831633	39.0	37.0	39.0	32.0	39.0
14-15	37.6702589486533	40.0	38.0	41.0	32.0	41.0
16-17	37.63438788104865	40.0	38.0	41.0	32.0	41.0
18-19	37.574643147856264	40.0	38.0	41.0	32.0	41.0
20-21	37.51071220454803	40.0	38.0	41.0	32.0	41.0
22-23	37.249864957413706	39.5	37.5	41.0	31.5	41.0
24-25	37.200797943566656	40.0	37.0	41.0	31.0	41.0
26-27	37.10961481202557	40.0	37.0	41.0	31.0	41.0
28-29	36.94927153311086	40.0	37.0	41.0	30.0	41.0
30-31	36.70739481609628	40.0	36.5	41.0	30.0	41.0
32-33	36.46943781929079	40.0	36.0	41.0	30.0	41.0
34-35	36.31229036481969	39.0	36.0	41.0	29.0	41.0
36-37	36.030969693083165	39.0	35.5	40.5	28.0	41.0
38-39	35.179819714295235	38.0	35.0	40.0	27.0	41.0
40-41	34.95080839918661	38.0	35.0	40.0	26.0	41.0
42-43	34.64419569281286	38.0	34.0	40.0	25.0	41.0
44-45	34.34883572819614	37.0	34.0	40.0	24.0	41.0
46-47	34.20547479740677	37.5	33.5	40.0	24.0	41.0
48-49	34.21524555592128	38.0	34.0	40.0	23.0	41.0
50-51	33.30984015448197	37.5	32.5	39.5	20.0	40.5
52-53	32.86516865391927	37.5	33.0	39.5	10.0	40.5
54-55	32.446572882568205	38.0	32.5	40.0	2.0	41.0
56-57	31.90597851615896	38.0	31.0	40.5	2.0	41.0
58-59	31.605337986536682	38.0	31.0	40.0	2.0	41.0
60-61	31.34408902347738	37.0	31.0	40.0	2.0	41.0
62-63	31.014074438437824	37.0	30.5	40.0	2.0	41.0
64-65	30.737613318742913	36.5	30.0	40.0	2.0	41.0
66-67	30.413968810995705	36.0	29.5	39.5	2.0	41.0
68-69	30.081216907962812	35.5	29.0	39.0	2.0	41.0
70-71	29.77481162641461	35.0	29.0	39.0	2.0	41.0
72-73	29.448134035031387	35.0	29.0	38.5	2.0	40.0
74-75	29.080940952968493	35.0	28.5	37.0	2.0	40.0
76-77	28.7052559998016	35.0	28.0	37.0	2.0	39.0
78-79	28.35552644450076	35.0	27.0	36.5	2.0	39.0
80-81	28.02051766838354	35.0	26.0	36.0	2.0	38.5
82-83	27.737379919956176	34.5	26.0	36.0	2.0	37.0
84-85	27.42932595222816	34.0	26.0	35.0	2.0	37.0
86-87	27.14523934711734	34.0	25.5	35.0	2.0	36.5
88-89	26.920692765165896	34.0	25.0	35.0	2.0	36.0
90-91	26.684517772866368	34.0	25.0	35.0	2.0	36.0
92-93	26.400086829338015	34.0	24.0	35.0	2.0	36.0
94-95	26.11266990749308	34.0	22.5	35.0	2.0	35.0
96-97	25.848991865454295	34.0	20.0	35.0	2.0	35.0
98-99	25.56721782336151	34.0	17.0	35.0	2.0	35.0
100-101	24.740215117226946	33.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5341371071833052
1101	2	0.5549829793850662
1101	3	0.4306408392043508
1101	4	0.5368827511910794
1101	5	0.47073942235488175
1101	6	0.4591081135997612
1101	7	0.43028208526549605
1101	8	0.4594859925892365
1101	9	0.5801497416193939
1101	10-11	0.6327537043706783
1101	12-13	0.6104912835159624
1101	14-15	0.7577167744179505
1101	16-17	0.7280644367522981
1101	18-19	0.7260973298518323
1101	20-21	0.7992513573656481
1101	22-23	0.795734907073566
1101	24-25	0.7280760830902935
1101	26-27	0.7998561527159396
1101	28-29	0.9094780227462493
1101	30-31	0.9394146851863852
1101	32-33	0.9697144698313238
1101	34-35	1.056035651511202
1101	36-37	0.9991400242118544
1101	38-39	1.078907225164592
1101	40-41	1.0025432177061475
1101	42-43	1.0692134742507662
1101	44-45	1.214785916085475
1101	46-47	1.162676922442543
1101	48-49	1.1013499604315768
1101	50-51	1.12352926957022
1101	52-53	0.9452570890003571
1101	54-55	0.8468473872397055
1101	56-57	0.8513482175934683
1101	58-59	0.9095787827549096
1101	60-61	0.8338423962363315
1101	62-63	0.8242122108607397
1101	64-65	0.8592241985957934
1101	66-67	0.8368695132522781
1101	68-69	0.8069364844424882
1101	70-71	0.9001949972474392
1101	72-73	0.8194834946112053
1101	74-75	0.8225188490276061
1101	76-77	0.778076807721952
1101	78-79	0.7827071492893367
1101	80-81	0.8069228388880703
1101	82-83	0.8174978469863383
1101	84-85	0.7831424936980049
1101	86-87	0.7936202928320348
1101	88-89	0.7610029109091165
1101	90-91	0.8441863279515722
1101	92-93	0.9559492221603598
1101	94-95	1.0041457062892327
1101	96-97	1.001423331738149
1101	98-99	0.9815601241129741
1101	100-101	1.1805681235994214
1102	1	0.489536457246853
1102	2	0.42121438505127884
1102	3	0.46122308784915944
1102	4	0.47696861142730285
1102	5	0.48842510789982185
1102	6	0.5363322704727125
1102	7	0.5245588547162043
1102	8	0.49275407748652356
1102	9	0.636165945648635
1102	10-11	0.5977288874409368
1102	12-13	0.5608002170787643
1102	14-15	0.7610826942572473
1102	16-17	0.7237161514395964
1102	18-19	0.7984426236410513
1102	20-21	0.7911766051894773
1102	22-23	0.8375992231792324
1102	24-25	0.8931867143826295
1102	26-27	0.930111463102115
1102	28-29	0.989909311972248
1102	30-31	0.9856639461073442
1102	32-33	1.061742598414945
1102	34-35	1.038074971853142
1102	36-37	1.0553828355257053
1102	38-39	1.0289871705170057
1102	40-41	1.0909318057318913
1102	42-43	1.0956015223185602
1102	44-45	1.168255798795606
1102	46-47	1.1877357215998217
1102	48-49	1.1122039031025466
1102	50-51	1.1579909131232853
1102	52-53	1.0015947911823915
1102	54-55	1.0064013233279425
1102	56-57	0.9339816830475414
1102	58-59	0.9890682891904028
1102	60-61	1.017770086225834
1102	62-63	1.0752121898376679
1102	64-65	1.0428080024129152
1102	66-67	0.9635110117135319
1102	68-69	0.8962181504105899
1102	70-71	0.9666725107121259
1102	72-73	0.9789361906958511
1102	74-75	0.8704049176201671
1102	76-77	0.9369901396877047
1102	78-79	0.9404268719413125
1102	80-81	0.9483389527517083
1102	82-83	0.9704015759410716
1102	84-85	0.9282382101507913
1102	86-87	0.9203418027825201
1102	88-89	0.9352205551620507
1102	90-91	0.9645721127889999
1102	92-93	0.925011464147584
1102	94-95	1.0323935894815754
1102	96-97	1.0556377490767836
1102	98-99	1.0720904871504011
1102	100-101	1.1059683717166529
1103	1	0.4343826967710811
1103	2	0.4276300606132537
1103	3	0.3479019277997395
1103	4	0.4439009325769021
1103	5	0.4419207793183162
1103	6	0.4960809387653242
1103	7	0.36188596242770643
1103	8	0.4347547661730573
1103	9	0.5251604189240666
1103	10-11	0.5539167171660182
1103	12-13	0.5536412354006899
1103	14-15	0.6117839962299101
1103	16-17	0.718434194852982
1103	18-19	0.7365231176910569
1103	20-21	0.7403363141544901
1103	22-23	0.8221291560219584
1103	24-25	0.7513135807643465
1103	26-27	0.7611367940448375
1103	28-29	0.8245291052003054
1103	30-31	0.9276133265720787
1103	32-33	1.011880568702047
1103	34-35	0.9675791459502818
1103	36-37	0.9873028937373434
1103	38-39	0.9030054303325841
1103	40-41	1.0469601523319554
1103	42-43	0.9352562901410693
1103	44-45	0.867922969482585
1103	46-47	1.0974979868882855
1103	48-49	1.1194285964711952
1103	50-51	1.0595846608046813
1103	52-53	0.980469741308589
1103	54-55	0.9427435359604743
1103	56-57	0.9571489402132372
1103	58-59	0.9730201229320237
1103	60-61	1.0368102064209062
1103	62-63	0.8146229809859662
1103	64-65	0.7348762376882831
1103	66-67	0.7095618102444305
1103	68-69	0.8848396085847945
1103	70-71	0.8777303823553275
1103	72-73	0.9376704456214249
1103	74-75	0.937241523528769
1103	76-77	0.9488600758131192
1103	78-79	0.9457996249732972
1103	80-81	0.9777886561502633
1103	82-83	1.0064237994125982
1103	84-85	1.0030985352418256
1103	86-87	1.0544820189520046
1103	88-89	1.0476867904837945
1103	90-91	1.0115306193566234
1103	92-93	1.0539131795251677
1103	94-95	0.9864088610736736
1103	96-97	0.9048369986516498
1103	98-99	1.0032027296825383
1103	100-101	1.0822549242064206
1104	1	0.3353704100052468
1104	2	0.3681824753807348
1104	3	0.39060761613800565
1104	4	0.3742310635167172
1104	5	0.4370904686081758
1104	6	0.4207755312286281
1104	7	0.4218058097506088
1104	8	0.3288697310480728
1104	9	0.530829363328003
1104	10-11	0.49967710039735636
1104	12-13	0.5291116469933996
1104	14-15	0.6346169745657804
1104	16-17	0.6268732992573618
1104	18-19	0.6657499129620135
1104	20-21	0.724868176492123
1104	22-23	0.6546597253277042
1104	24-25	0.6727154887109563
1104	26-27	0.6949243711340927
1104	28-29	0.7201635953308241
1104	30-31	0.8236493262130793
1104	32-33	0.8316288994023111
1104	34-35	0.8628511971216284
1104	36-37	0.8658034804555328
1104	38-39	0.9032539815302769
1104	40-41	0.8165310480493204
1104	42-43	0.9658105871906102
1104	44-45	0.910812837557998
1104	46-47	1.0621373844201116
1104	48-49	0.87167965281715
1104	50-51	0.8750163891845872
1104	52-53	0.7437925999810986
1104	54-55	0.692825162571097
1104	56-57	0.6790100220868993
1104	58-59	0.6964079138709316
1104	60-61	0.6718301637824275
1104	62-63	0.5921154585917101
1104	64-65	0.5346251733692924
1104	66-67	0.6220742317437349
1104	68-69	0.6187134560371987
1104	70-71	0.5088183015199874
1104	72-73	0.5451800803304891
1104	74-75	0.6463675315920874
1104	76-77	0.5751035967817124
1104	78-79	0.4967950095573528
1104	80-81	0.5641776423975351
1104	82-83	0.5920256453634885
1104	84-85	0.5707475234694286
1104	86-87	0.5740791987396783
1104	88-89	0.5933207149600008
1104	90-91	0.5272384867030375
1104	92-93	0.40924936835329007
1104	94-95	0.31915965307051763
1104	96-97	0.5027056239834558
1104	98-99	0.7455066606998635
1104	100-101	0.6827318542916565
1105	1	0.1722040726384897
1105	2	0.14566071065873842
1105	3	0.17719750178044436
1105	4	0.18708762371767307
1105	5	0.15373978723381754
1105	6	0.14100338467705598
1105	7	0.14629866549324078
1105	8	0.09991742176514151
1105	9	0.09203142613608861
1105	10-11	0.1161359325060971
1105	12-13	0.10479392149441935
1105	14-15	0.002798117349477991
1105	16-17	0.034528668172590926
1105	18-19	-0.07163696787730345
1105	20-21	0.0078110831607460796
1105	22-23	0.003286154386898943
1105	24-25	-0.008311415130975774
1105	26-27	-0.3175183646870252
1105	28-29	-0.1293248468937378
1105	30-31	-0.24899058282280606
1105	32-33	-0.24289246084550342
1105	34-35	-0.1424550723102911
1105	36-37	-0.10982028479802608
1105	38-39	0.0604111922237891
1105	40-41	0.06762212305923043
1105	42-43	0.10287528633946152
1105	44-45	0.12003831873404636
1105	46-47	0.15418468133135121
1105	48-49	0.19532991897716556
1105	50-51	0.13979907945388703
1105	52-53	-0.03948339523004307
1105	54-55	0.031035596650980324
1105	56-57	0.08275071843696225
1105	58-59	0.08915728071957929
1105	60-61	0.021185679869635266
1105	62-63	0.09961623127793828
1105	64-65	0.0439979573139162
1105	66-67	-0.021820046828910478
1105	68-69	0.020060212483528517
1105	70-71	0.04650428860779243
1105	72-73	0.05442967813871391
1105	74-75	0.10519856324539845
1105	76-77	0.05296312822149929
1105	78-79	0.0789371614605443
1105	80-81	0.13232332829517546
1105	82-83	0.07329605948740081
1105	84-85	0.03309485703261217
1105	86-87	0.08318496099400008
1105	88-89	0.17482858712728344
1105	90-91	0.1394255180071653
1105	92-93	0.17510907500025752
1105	94-95	0.20288038267506536
1105	96-97	0.2250442931002894
1105	98-99	0.2317059128072998
1105	100-101	0.22579262676861944
1106	1	-0.15431417185172336
1106	2	-0.09967667837408811
1106	3	-0.17453989110719093
1106	4	-0.15734616002580282
1106	5	-0.18941090805969907
1106	6	-0.1667599182987729
1106	7	-0.19454444940439686
1106	8	-0.19998212374464686
1106	9	-0.1963290302928442
1106	10-11	-0.35910927440459517
1106	12-13	-0.3314959474314847
1106	14-15	-0.36994995055757585
1106	16-17	-0.3661686899185739
1106	18-19	-0.41505683609474175
1106	20-21	-0.2587713703399359
1106	22-23	-0.29974975296053685
1106	24-25	-0.4134911352145991
1106	26-27	-0.3401331690024776
1106	28-29	-0.39015254554940526
1106	30-31	-0.36133547872552896
1106	32-33	-0.3123180812800044
1106	34-35	-0.30392174609769995
1106	36-37	-0.39513985225956816
1106	38-39	-0.36615833691984534
1106	40-41	-0.4181306518500705
1106	42-43	-0.46418326075311
1106	44-45	-0.422485464996015
1106	46-47	-0.4229172794177174
1106	48-49	-0.5700750583795084
1106	50-51	-0.728797788047963
1106	52-53	-0.7378088035159536
1106	54-55	-0.6560732589610794
1106	56-57	-0.6224386484819604
1106	58-59	-0.6093151295207448
1106	60-61	-0.736413906622726
1106	62-63	-0.5664702418768854
1106	64-65	-0.5407541293709635
1106	66-67	-0.6755219272205579
1106	68-69	-0.7576849745291909
1106	70-71	-0.6452343936403011
1106	72-73	-0.7021651765063872
1106	74-75	-0.7965183561142766
1106	76-77	-0.8132280991611829
1106	78-79	-0.7367179337381486
1106	80-81	-0.7747317574151182
1106	82-83	-0.8325266653299046
1106	84-85	-0.8976382249146901
1106	86-87	-0.8090634687055918
1106	88-89	-0.8367369243087701
1106	90-91	-0.787511346603484
1106	92-93	-0.7861490175791985
1106	94-95	-0.783046805016145
1106	96-97	-0.7304396023421837
1106	98-99	-0.7528841429380044
1106	100-101	-0.9435428636870107
1107	1	-0.38633887858386373
1107	2	-0.4365898021269814
1107	3	-0.39425788409375073
1107	4	-0.3414707062797717
1107	5	-0.37049716266998445
1107	6	-0.37854969887435175
1107	7	-0.33237894707790616
1107	8	-0.30945153163182226
1107	9	-0.40761129199893986
1107	10-11	-0.4117041818804523
1107	12-13	-0.4664229149891881
1107	14-15	-0.7032044960440444
1107	16-17	-0.5376355996979854
1107	18-19	-0.4871521041035365
1107	20-21	-0.40533901061982647
1107	22-23	-0.4553965387477632
1107	24-25	-0.4631302166305531
1107	26-27	-0.39614138256492026
1107	28-29	-0.44182035445453494
1107	30-31	-0.4260478873322171
1107	32-33	-0.39670252153095475
1107	34-35	-0.49892762160783377
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2308	50-51	-2.4134362365582405
2308	52-53	-2.4172377537782666
2308	54-55	-2.1066909597636396
2308	56-57	-1.9815396548666016
2308	58-59	-1.872962408837214
2308	60-61	-1.8423885054751743
2308	62-63	-1.9495806950710701
2308	64-65	-1.9105230254600194
2308	66-67	-2.0212804765378216
2308	68-69	-1.8019274732847492
2308	70-71	-1.7756000013978124
2308	72-73	-1.742057660666351
2308	74-75	-1.5817391187364187
2308	76-77	-1.516405494043493
2308	78-79	-1.6311336710656974
2308	80-81	-2.1430990356602173
2308	82-83	-1.99259766635139
2308	84-85	-1.7428283593910372
2308	86-87	-1.6224176576895672
2308	88-89	-1.5564666153271638
2308	90-91	-1.7385020806622684
2308	92-93	-1.7608932938543447
2308	94-95	-1.846578520859378
2308	96-97	-1.7274431265855803
2308	98-99	-1.7444257809810573
2308	100-101	-1.8750736768702971
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67831.0
3	10501.0
4	9090.0
5	8646.0
6	9962.0
7	12429.0
8	15435.0
9	17165.0
10	20199.0
11	23919.0
12	24476.0
13	26944.0
14	30720.0
15	36140.0
16	46294.0
17	64209.0
18	100929.0
19	166697.0
20	240541.0
21	167687.0
22	85884.0
23	70228.0
24	70957.0
25	74900.0
26	79794.0
27	88367.0
28	100606.0
29	117646.0
30	137873.0
31	165143.0
32	203698.0
33	254765.0
34	336587.0
35	480585.0
36	773878.0
37	1148906.0
38	1230175.0
39	294278.0
40	433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.661504958798542	47.525181605315225	24.732488572769277	17.08082486311696
2	16.382863999302018	48.70562570714432	19.62234325613272	15.289167037420942
3	42.26089452000312	21.56218772195424	20.248130517462897	15.928787240579743
4	16.31056306263978	20.337783551654795	21.26086810971828	42.090785275987145
5	16.080260748441553	46.32821674831701	22.1315997102534	15.459922792988037
6	41.99132223442999	21.286734746736595	20.384311109356666	16.33763190947676
7	16.652124006190387	20.69680328549664	47.35435107303116	15.296721635281814
8	41.68404712064628	21.35998114516695	21.158048935688115	15.797922798498657
9	41.2465859775955	21.067038851978555	21.825865411568564	15.860509758857381
10-11	15.775000341219442	33.95665441750026	34.16953865810795	16.098806583172347
12-13	15.6193685727059	21.388100319873143	34.17193746490334	28.82059364251762
14-15	15.770302088257562	46.83392403302126	21.257468991385114	16.138304887336062
16-17	28.58325434633915	21.19813548136868	21.380124744420392	28.83848542787178
18-19	15.951944364324977	33.891767034489	21.356908340648033	28.799380260537987
20-21	28.71573718589554	33.934650497742865	21.362591968244242	15.987020348117346
22-23	28.6475866824047	21.112479682077385	34.20755644389157	16.032377191626342
24-25	15.893232093766443	33.703797811816	21.773012651692977	28.629957442724578
26-27	16.105519248129205	26.055800383759987	41.605541957340286	16.233138410770522
28-29	26.109401294243117	21.05626938218767	36.67910232841226	16.15522699515695
30-31	23.75316023100367	22.172433870412092	37.20542207771958	16.86898382086466
32-33	18.81380413872933	23.814745512771506	38.6964897093803	18.67496063911887
34-35	18.229519705714843	23.179886894451894	39.60364190649047	18.98695149334279
36-37	18.17243644006704	23.562660966658783	39.417324266286265	18.847578326987914
38-39	18.556733709158706	23.749008523942543	38.896898963728674	18.79735880317008
40-41	19.055368545929966	23.883856309689115	38.60858968522802	18.4521854591529
42-43	19.09942437596216	23.709993080126065	38.53500303510323	18.65557950880855
44-45	18.70206936841241	23.83807369919644	38.72859233890586	18.73126459348529
46-47	19.042031122593592	23.663057491824162	38.5067676522431	18.788143733339144
48-49	19.235323256011384	24.22300842333528	38.046614468437056	18.495053852216284
50-51	19.28336299931188	24.524827041401615	37.351066588418334	18.84074337086817
52-53	19.29843728348973	25.18180066896945	36.21782019054594	19.301941856994887
54-55	20.32272814812817	25.80457007899496	34.45053837622945	19.422163396647406
56-57	20.532453646857192	26.42029038852011	33.02824663753333	20.019009327089364
58-59	20.85913047438921	27.068060961781526	32.49856837299953	19.574240190829727
60-61	20.846419899052684	26.83150830251052	32.16823416533506	20.15383763310174
62-63	20.236113616850478	27.08336883810939	32.626058605505605	20.054458939534534
64-65	20.482518842297395	26.928770352909755	32.462208337309356	20.126502467483498
66-67	20.18027441553376	26.458574991369556	32.44571413710256	20.915436455994122
68-69	20.386428293636538	26.82576097513915	32.519414398650156	20.268396332574152
70-71	19.91881843509607	26.811662398650693	32.825498572515976	20.44402059373726
72-73	20.111823853336094	26.512105704923545	32.78774785977766	20.588322581962704
74-75	20.344470322589032	26.292353259276112	32.52658464495562	20.836591773179233
76-77	20.580159350478038	26.59641062467791	32.46039712569015	20.363032899153904
78-79	20.73602758732992	26.926694554003706	31.523718222772334	20.813559635894038
80-81	20.558857831679756	26.765350673362835	31.729232673716545	20.946558821240867
82-83	21.152536306834477	26.879082902760214	31.28855911584807	20.679821674557243
84-85	21.293854400239553	27.04080350763206	31.432418267579926	20.232923824548458
86-87	21.13631406392649	27.456798130276606	31.032218758166135	20.374669047630768
88-89	21.034885308920597	27.13800535087662	30.740021432010007	21.087087908192775
90-91	21.314645611718053	27.488604322048083	30.920157738723674	20.27659232751019
92-93	21.330149346912673	27.750011261329945	30.654192517818068	20.265646873939314
94-95	21.265884423817454	27.60777788344081	30.47465760401223	20.65168008872951
96-97	20.730822376171467	27.128343902094514	31.20935113053629	20.93148259119773
98-99	20.70188347567175	27.438885005562675	30.799729335469202	21.059502183296377
100-101	21.241524324792945	26.97690325699562	30.457159314407967	21.324413103803465
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4911.0
1	2914.0
2	717.0
3	442.5
4	356.0
5	355.5
6	456.0
7	576.5
8	584.5
9	666.5
10	1092.5
11	4904.0
12	76948.0
13	86161.5
14	24667.0
15	23790.5
16	27205.5
17	31580.0
18	35932.5
19	40191.0
20	44781.5
21	49801.5
22	54910.0
23	60518.0
24	67157.0
25	74583.5
26	85389.0
27	97279.0
28	106444.0
29	114256.0
30	121424.5
31	130665.0
32	142728.0
33	155060.0
34	170745.5
35	180932.0
36	186891.0
37	193846.0
38	196805.5
39	201354.5
40	206801.5
41	212942.0
42	215678.5
43	214530.5
44	216479.5
45	218292.5
46	215941.5
47	215065.0
48	212803.5
49	206474.0
50	198978.5
51	188665.0
52	180089.5
53	171859.0
54	164962.5
55	161925.0
56	157265.5
57	141498.5
58	137688.5
59	115888.5
60	73048.0
61	52684.0
62	38062.0
63	27210.5
64	20107.0
65	14529.0
66	9743.5
67	6608.0
68	4479.5
69	3054.0
70	2242.5
71	1754.0
72	1433.0
73	1219.0
74	1057.0
75	885.0
76	784.0
77	698.0
78	615.0
79	541.0
80	439.5
81	365.0
82	333.5
83	286.5
84	233.0
85	180.5
86	132.5
87	105.5
88	81.5
89	58.5
90	46.0
91	32.5
92	21.0
93	15.5
94	11.0
95	9.0
96	5.5
97	3.5
98	3.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052725673734470103
2	0.09280188163005537
3	0.08336614319107281
4	0.055249697080512084
5	0.08524448614626687
6	0.001320709890370807
7	0.001217988010008633
8	0.0051214194637712405
9	0.01037490991657956
10-11	0.010565679122966456
12-13	0.01747739421590701
14-15	0.012414672969485587
16-17	0.0015261536510951545
18-19	5.943194506668631E-4
20-21	6.603549451854035E-5
22-23	4.1822479861742217E-4
24-25	7.850886570537574E-4
26-27	0.001489467265251521
28-29	0.0016068636999511483
30-31	0.0049820111975654325
32-33	1.467455433745341E-4
34-35	0.0025533724547168934
36-37	3.4485202693015514E-4
38-39	0.0026707688894165205
40-41	0.003683313138700806
42-43	0.0012693489501897199
44-45	6.823667766915836E-4
46-47	0.0011519525154900928
48-49	0.0025460351775481666
50-51	0.004607810061960371
52-53	0.1338906337749249
54-55	0.1444343010663852
56-57	0.13075761642387862
58-59	0.12399264687431258
60-61	0.11152661296464592
62-63	0.11623714490696846
64-65	0.11869513275849192
66-67	0.1272357233828898
68-69	0.1405895678299724
70-71	0.1271036523938527
72-73	0.11883454102469772
74-75	0.12434483617841147
76-77	0.1359744204908433
78-79	0.1501793890894982
80-81	0.1398558401130997
82-83	0.11864377181831083
84-85	0.1255848360199263
86-87	0.12606175903589353
88-89	0.12242246956020507
90-91	0.13333300071010168
92-93	0.1505389156707658
94-95	0.16021678425631633
96-97	0.16106790840788862
98-99	0.14356850236047544
100-101	0.13224708368913013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6814517.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.94332131656885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.14607744559797	51.901603747295546
2	5.562349734445049	6.334773364072717
3	1.275066351419606	2.178195388464952
4	0.5581280210157646	1.2712665294592551
5	0.35309032320665945	1.0053067864063978
6	0.24214928164400273	0.8273270610738465
7	0.17878824834319731	0.7126557671123234
8	0.13819546831281482	0.6295447165304258
9	0.10306839086024866	0.5282150848503163
>10	0.41456978471872685	3.527022515031712
>50	0.011301443670673152	0.4858910839558017
>100	0.011874897696211632	1.4823125001338606
>500	0.0024687721168699947	1.035099764756727
>1k	0.00241838901241045	2.741139497295753
>5k	3.022986265513062E-4	1.3446628474708475
>10k+	1.511493132756531E-4	23.994983346089537
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	783054	11.490968472160242	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA	33451	0.49087851714215397	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	19854	0.2913486018158	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	9609	0.14100779262858984	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTGCAGAGGACTCTGAGA	9412	0.13811690542411148	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC	7398	0.10856235298848033	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA	7396	0.10853300387980543	No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT	7037	0.10326483887265964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.467455433745341E-5	0.0	0.0	0.0	0.0
2	1.467455433745341E-5	0.0	0.0	0.0	0.0
3	1.467455433745341E-5	0.0	0.0	0.0	0.0
4	1.467455433745341E-5	0.0	0.0	0.0	0.0
5	1.467455433745341E-5	0.0	0.0	1.467455433745341E-5	0.0
6	1.467455433745341E-5	1.467455433745341E-5	0.0	2.934910867490682E-5	0.0
7	1.467455433745341E-5	1.467455433745341E-5	0.0	4.402366301236023E-5	0.0
8	1.467455433745341E-5	1.467455433745341E-5	0.0	4.402366301236023E-5	0.0
9	1.467455433745341E-5	1.467455433745341E-5	0.0	4.402366301236023E-5	0.0
10-11	1.467455433745341E-5	1.467455433745341E-5	0.0	4.402366301236023E-5	0.0
12-13	1.467455433745341E-5	1.467455433745341E-5	0.0	8.071004885599376E-5	0.0
14-15	1.467455433745341E-5	1.467455433745341E-5	0.0	8.804732602472046E-5	0.0
16-17	1.467455433745341E-5	1.467455433745341E-5	0.0	9.538460319344717E-5	0.0
18-19	1.467455433745341E-5	2.2011831506180116E-5	0.0	1.320709890370807E-4	0.0
20-21	1.467455433745341E-5	3.668638584363353E-5	0.0	1.320709890370807E-4	0.0
22-23	1.467455433745341E-5	5.136094018108694E-5	0.0	1.467455433745341E-4	0.0
24-25	1.467455433745341E-5	5.869821734981364E-5	0.0	1.467455433745341E-4	0.0
26-27	1.467455433745341E-5	5.869821734981364E-5	0.0	1.687573748807142E-4	0.0
28-29	1.467455433745341E-5	5.869821734981364E-5	0.0	1.9076920638689434E-4	0.0
30-31	1.467455433745341E-5	5.869821734981364E-5	0.0	2.2011831506180116E-4	0.0
32-33	1.467455433745341E-5	5.869821734981364E-5	0.0	2.2745559223052785E-4	0.0
34-35	1.467455433745341E-5	7.337277168726705E-5	0.0	2.3479286939925457E-4	0.0
36-37	1.467455433745341E-5	7.337277168726705E-5	0.0	2.3479286939925457E-4	0.0
38-39	1.467455433745341E-5	7.337277168726705E-5	0.0	2.6414197807416134E-4	0.0
40-41	1.467455433745341E-5	7.337277168726705E-5	0.0	2.861538095803415E-4	7.337277168726705E-6
42-43	1.467455433745341E-5	7.337277168726705E-5	0.0	2.934910867490682E-4	1.467455433745341E-5
44-45	4.402366301236023E-5	7.337277168726705E-5	0.0	3.155029182552483E-4	1.467455433745341E-5
46-47	5.136094018108694E-5	7.337277168726705E-5	0.0	3.301774725927017E-4	1.467455433745341E-5
48-49	5.869821734981364E-5	7.337277168726705E-5	0.0	3.375147497614284E-4	2.2011831506180116E-5
50-51	6.603549451854035E-5	8.804732602472046E-5	0.0	3.375147497614284E-4	2.934910867490682E-5
52-53	9.538460319344717E-5	8.804732602472046E-5	0.0	3.7420113560506196E-4	2.934910867490682E-5
54-55	1.467455433745341E-4	8.804732602472046E-5	0.0	3.9621296711124206E-4	3.668638584363353E-5
56-57	1.687573748807142E-4	8.804732602472046E-5	0.0	3.9621296711124206E-4	5.869821734981364E-5
58-59	1.9810648355562103E-4	1.0272188036217388E-4	0.0	4.035502442799688E-4	5.869821734981364E-5
60-61	2.4213014656798126E-4	1.1005915753090058E-4	0.0	4.402366301236023E-4	5.869821734981364E-5
62-63	2.6414197807416134E-4	1.1739643469962729E-4	0.0	4.549111844610557E-4	5.869821734981364E-5
64-65	2.934910867490682E-4	1.1739643469962729E-4	0.0	4.549111844610557E-4	5.869821734981364E-5
66-67	3.301774725927017E-4	1.1739643469962729E-4	0.0	4.6958573879850914E-4	5.869821734981364E-5
68-69	3.9621296711124206E-4	1.24733711868354E-4	0.0	5.136094018108693E-4	5.869821734981364E-5
70-71	4.915975703046893E-4	1.394082662058074E-4	0.0	5.136094018108693E-4	5.869821734981364E-5
72-73	6.016567278355899E-4	1.467455433745341E-4	0.0	5.136094018108693E-4	5.869821734981364E-5
74-75	7.484022712101239E-4	1.467455433745341E-4	0.0	5.502957876545029E-4	5.869821734981364E-5
76-77	9.391714775970182E-4	1.7609465204944093E-4	0.0	5.576330648232296E-4	5.869821734981364E-5
78-79	0.001144615238321366	1.7609465204944093E-4	0.0	5.796448963294097E-4	7.337277168726705E-5
80-81	0.0014014199392268006	1.7609465204944093E-4	0.0	5.869821734981364E-4	7.337277168726705E-5
82-83	0.0017609465204944093	1.9076920638689434E-4	0.0	5.869821734981364E-4	7.337277168726705E-5
84-85	0.0022231949821241914	1.9076920638689434E-4	0.0	5.869821734981364E-4	7.337277168726705E-5
86-87	0.002854200818634688	1.9810648355562103E-4	0.0	6.089940050043165E-4	8.804732602472046E-5
88-89	0.0037420113560506195	2.0544376072434775E-4	0.0	6.236685593417699E-4	9.538460319344717E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGTA	177115	0.0	90.9314	5
TGGTATC	177570	0.0	90.78486	7
AAGCAGT	178135	0.0	90.74073	1
GTGGTAT	177825	0.0	90.6994	6
GTATCAA	177970	0.0	90.64816	9
GGTATCA	178125	0.0	90.55596	8
GCAGTGG	178920	0.0	90.15675	3
AGCAGTG	179375	0.0	90.06578	2
CAGTGGT	180270	0.0	89.35774	4
TCAACGC	176670	0.0	46.070377	12-13
CAACGCA	176710	0.0	46.020996	12-13
ATCAACG	176760	0.0	46.004623	10-11
AACGCAG	177545	0.0	45.873203	14-15
ACGCAGA	177570	0.0	45.84135	14-15
CGCAGAG	177785	0.0	45.82041	16-17
TATCAAC	178035	0.0	45.663162	10-11
AGAGTAC	173010	0.0	45.59466	18-19
CAGAGTA	177975	0.0	45.252003	18-19
GAGTACT	103085	0.0	45.211456	20-21
GCAGAGT	180700	0.0	44.936794	16-17
>>END_MODULE