FastQCFastQC Report
Sat 18 Jun 2016
SRR578581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6814517
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91020.13356779357950094No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72080.10577418766436418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG2900.027.7966443
CGCGTAG1500.026.86447154-55
ACTCGCG1900.022.4562950-51
CTCGCGT2100.020.31759552-53
CGTAGCG2100.019.1889856-57
GCGACGA2350.019.16496360-61
CAACGCA57550.018.16403412-13
ATCAACG58650.017.70211410-11
CGTACGC2300.017.52242920-21
ACGTACG2300.017.52242920-21
TCGCGTA2600.017.3220552-53
ACGCAGA63100.016.5288414-15
GTATCAA77200.015.9676199
GGTATCA77850.015.7743248
GTGGTAT76950.015.7739686
AGTGGTA77750.015.6117775
AAGCAGT84200.015.433331
TGGTATC80250.015.3025697
TCAACGC55900.014.71420310-11
AACGCAG61850.013.52863712-13
Produced by FastQC (version 0.11.5)