##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578581_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6814517 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0072360227438 31.0 31.0 34.0 30.0 34.0 2 32.27649487116989 34.0 31.0 34.0 30.0 34.0 3 32.34790565494224 34.0 31.0 34.0 30.0 34.0 4 35.914955674774895 37.0 35.0 37.0 35.0 37.0 5 35.796563424817926 37.0 35.0 37.0 33.0 37.0 6 35.774137477388344 37.0 35.0 37.0 33.0 37.0 7 35.770361567811776 37.0 35.0 37.0 33.0 37.0 8 35.68912587641942 37.0 35.0 37.0 33.0 37.0 9 37.33188441675323 39.0 37.0 39.0 34.0 39.0 10-11 37.32753326464663 39.0 37.0 39.0 34.0 39.0 12-13 37.322543549308044 39.0 37.0 39.0 34.0 39.0 14-15 38.64137825175284 40.0 38.0 41.0 34.0 41.0 16-17 38.60077126522687 40.0 38.0 41.0 34.0 41.0 18-19 38.56904084324685 40.0 38.0 41.0 34.0 41.0 20-21 38.51471586320791 40.0 38.0 41.0 34.0 41.0 22-23 38.400084334663774 40.0 38.0 41.0 34.0 41.0 24-25 38.31927545268432 40.0 38.0 41.0 33.5 41.0 26-27 38.15477692696342 40.0 38.0 41.0 33.0 41.0 28-29 38.05907572026015 40.0 37.5 41.0 33.0 41.0 30-31 37.963118956193085 40.0 37.0 41.0 33.0 41.0 32-33 37.833275271013335 40.0 37.0 41.0 33.0 41.0 34-35 37.702646497176545 40.0 37.0 41.0 32.0 41.0 36-37 37.57947635907284 40.0 37.0 41.0 32.0 41.0 38-39 37.451724751732215 40.0 37.0 41.0 31.0 41.0 40-41 37.30801163163875 40.0 36.0 41.0 31.0 41.0 42-43 37.15429229980643 39.5 36.0 41.0 31.0 41.0 44-45 37.00287900081547 39.0 36.0 41.0 30.5 41.0 46-47 37.08114192099015 39.0 36.0 41.0 31.0 41.0 48-49 37.06337521793547 39.0 36.0 41.0 31.0 41.0 50-51 37.047675352486465 39.5 36.0 41.0 31.0 41.0 52-53 37.068125297801735 40.0 36.0 41.0 31.0 41.0 54-55 36.95506365308062 39.0 36.0 41.0 31.0 41.0 56-57 36.82538300219957 39.0 35.5 41.0 31.0 41.0 58-59 36.627582262983566 39.0 35.0 41.0 30.0 41.0 60-61 36.42305889030727 39.0 35.0 41.0 30.0 41.0 62-63 36.17555147342064 38.5 35.0 40.5 29.5 41.0 64-65 35.88907445678102 38.0 35.0 40.0 29.0 41.0 66-67 35.59249555030826 37.5 34.0 40.0 29.0 41.0 68-69 35.27734643555809 37.0 34.0 40.0 28.5 41.0 70-71 34.92625933723549 36.5 34.0 39.0 28.0 41.0 72-73 34.51502212115693 36.0 34.0 39.0 28.0 41.0 74-75 34.129797827197436 36.0 34.0 39.0 28.0 40.0 76-77 32.67073220303068 34.5 31.5 37.0 26.0 39.0 78-79 33.26124221276431 35.0 33.0 37.0 26.0 39.0 80-81 33.189892255019686 35.0 33.0 37.0 27.0 39.0 82-83 32.8945112324175 35.0 33.0 36.0 26.0 38.5 84-85 32.583954959683865 35.0 33.0 36.0 26.0 37.0 86-87 32.27876106259622 35.0 33.0 35.5 26.0 37.0 88-89 32.008027494831985 35.0 32.5 35.0 25.5 36.5 90-91 31.75126652409848 35.0 32.0 35.0 25.0 36.0 92-93 31.502828153484685 35.0 32.0 35.0 25.0 36.0 94-95 31.245713232500556 35.0 32.0 35.0 24.0 36.0 96-97 30.648136617752954 35.0 32.0 35.0 20.5 35.5 98-99 30.627159415700334 34.0 32.0 35.0 19.5 35.0 100-101 30.054186085382135 34.0 31.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42858178123137236 1101 2 0.3994221774587885 1101 3 0.3875173896316326 1101 4 0.2852400482431676 1101 5 0.2970149009610594 1101 6 0.31250284794760574 1101 7 0.30383737097361063 1101 8 0.3832316553300359 1101 9 0.4738248686862008 1101 10-11 0.434485004329801 1101 12-13 0.4094344773504517 1101 14-15 0.5492327760775453 1101 16-17 0.5306248922686905 1101 18-19 0.5365943869856764 1101 20-21 0.6064599804404835 1101 22-23 0.6288727809137882 1101 24-25 0.709034450173796 1101 26-27 0.7035517082821912 1101 28-29 0.6988578903725866 1101 30-31 0.6606431084854805 1101 32-33 0.657403600747088 1101 34-35 0.6425237207000905 1101 36-37 0.6947224789957289 1101 38-39 0.707655513718521 1101 40-41 0.7083173927150668 1101 42-43 0.7402920730824434 1101 44-45 0.7814834042454422 1101 46-47 0.8239145532585894 1101 48-49 0.7547022019535348 1101 50-51 0.7698162091524026 1101 52-53 0.7359350936274609 1101 54-55 0.811444527925147 1101 56-57 0.7994467722853997 1101 58-59 0.8096626059567313 1101 60-61 0.7508402364989237 1101 62-63 0.7979268485508442 1101 64-65 0.7978380501509932 1101 66-67 0.7365601042487455 1101 68-69 0.7204922190831198 1101 70-71 0.7244323143027955 1101 72-73 0.706802496125043 1101 74-75 0.7312009592566753 1101 76-77 0.7069894900514839 1101 78-79 0.6257199201314663 1101 80-81 0.6017620165874078 1101 82-83 0.5823215557307364 1101 84-85 0.553582458538429 1101 86-87 0.5494633896289116 1101 88-89 0.5292642422484093 1101 90-91 0.5363339180584497 1101 92-93 0.564374645721287 1101 94-95 0.5911179182868231 1101 96-97 0.8957134370674993 1101 98-99 0.7005577634819602 1101 100-101 0.689360397317369 1102 1 0.4036613009158998 1102 2 0.3897348659047708 1102 3 0.37259944868888795 1102 4 0.26262772068192675 1102 5 0.3257565982915267 1102 6 0.312258969100931 1102 7 0.2730413174453332 1102 8 0.30920888112765965 1102 9 0.41803993529147476 1102 10-11 0.3906789447498582 1102 12-13 0.4034321128040972 1102 14-15 0.5230446723028379 1102 16-17 0.5538166769377639 1102 18-19 0.5559485695926085 1102 20-21 0.5855285330067517 1102 22-23 0.5947921248859984 1102 24-25 0.6306197206524544 1102 26-27 0.735093681090838 1102 28-29 0.6946349554549087 1102 30-31 0.7206222058708889 1102 32-33 0.7668855138305162 1102 34-35 0.8092038154879475 1102 36-37 0.807836151006093 1102 38-39 0.8202321940927746 1102 40-41 0.790022502561726 1102 42-43 0.79260656230759 1102 44-45 0.8411886416939538 1102 46-47 0.8726456112843053 1102 48-49 0.8780256974103722 1102 50-51 0.8411829424465296 1102 52-53 0.7763148152657138 1102 54-55 0.7733036757895491 1102 56-57 0.7483806958819414 1102 58-59 0.7740628010800634 1102 60-61 0.8434265373893552 1102 62-63 0.827517213647802 1102 64-65 0.790130109704414 1102 66-67 0.8042013725819785 1102 68-69 0.8410799182467841 1102 70-71 0.8058433826301084 1102 72-73 0.8102237654528608 1102 74-75 0.6674086797771892 1102 76-77 0.6390891511016434 1102 78-79 0.6382783579987503 1102 80-81 0.632355587294164 1102 82-83 0.6747200336606127 1102 84-85 0.6590843136482789 1102 86-87 0.6191669759082217 1102 88-89 0.5911265900095302 1102 90-91 0.565802481634389 1102 92-93 0.5642403767047597 1102 94-95 0.6203405232239447 1102 96-97 1.0625951366049655 1102 98-99 0.8107999799020895 1102 100-101 0.7858668108869331 1103 1 0.30299396317184346 1103 2 0.26702534072046546 1103 3 0.24006803160510515 1103 4 0.18160342781462901 1103 5 0.17841252597376211 1103 6 0.27901468784187955 1103 7 0.26809927334772254 1103 8 0.016652615303044627 1103 9 0.14347453390686127 1103 10-11 0.35214507229373737 1103 12-13 0.2999432777946467 1103 14-15 0.4192228701217502 1103 16-17 0.4187937354709632 1103 18-19 0.346853862621991 1103 20-21 0.3581591355087639 1103 22-23 0.3665591589247512 1103 24-25 0.18138179897704987 1103 26-27 0.21124640870224454 1103 28-29 0.42284880593967245 1103 30-31 0.26607353721433213 1103 32-33 0.47112695214150335 1103 34-35 0.4678361487773728 1103 36-37 0.4960152043204644 1103 38-39 0.5747416695432008 1103 40-41 0.40658873049149236 1103 42-43 0.5407873565006298 1103 44-45 0.5785056388541605 1103 46-47 0.4659163735302698 1103 48-49 0.5173401005676013 1103 50-51 0.47495772849615037 1103 52-53 0.5221808584290812 1103 54-55 0.6180639687347167 1103 56-57 0.5267317225881598 1103 58-59 0.2873239650573538 1103 60-61 0.2778467196048098 1103 62-63 0.13838563997316555 1103 64-65 0.3424190522215369 1103 66-67 0.3458987061287857 1103 68-69 0.30283599280083706 1103 70-71 0.30651818128276176 1103 72-73 0.47480255042817987 1103 74-75 0.37041972161863157 1103 76-77 0.18526261899124563 1103 78-79 0.3120367239836668 1103 80-81 0.32832589982599814 1103 82-83 0.3566241209509329 1103 84-85 0.35100717424997185 1103 86-87 0.17309197491771044 1103 88-89 0.22046354987013927 1103 90-91 0.30450395157400223 1103 92-93 0.26605306593467404 1103 94-95 0.32202454073078 1103 96-97 0.3739265445680253 1103 98-99 0.1698397571049135 1103 100-101 0.38067863400626933 1104 1 0.33612743916499 1104 2 0.3227334445642569 1104 3 0.2987094355397062 1104 4 0.18609508936975772 1104 5 0.2518959609758227 1104 6 0.2086015544046731 1104 7 0.20272155148651905 1104 8 0.2738809716721562 1104 9 0.27427754107601743 1104 10-11 0.3367112186697696 1104 12-13 0.3452433180800014 1104 14-15 0.38871359376815207 1104 16-17 0.4340085057932228 1104 18-19 0.42830422343941166 1104 20-21 0.3905831786398508 1104 22-23 0.5103085306444299 1104 24-25 0.4205947443368885 1104 26-27 0.506199463165494 1104 28-29 0.48513578187307616 1104 30-31 0.5042320679224161 1104 32-33 0.5600425491917775 1104 34-35 0.5123409679904896 1104 36-37 0.5667271406068792 1104 38-39 0.5284942364016558 1104 40-41 0.5323407221064471 1104 42-43 0.6508097569500535 1104 44-45 0.5797285969417558 1104 46-47 0.4434015164851175 1104 48-49 0.544089464637473 1104 50-51 0.449319554609275 1104 52-53 0.5426483135352527 1104 54-55 0.6158805727250893 1104 56-57 0.5880655817824803 1104 58-59 0.571008345251613 1104 60-61 0.4653140018901283 1104 62-63 0.32751951763474096 1104 64-65 0.5430858643780851 1104 66-67 0.5714920128544563 1104 68-69 0.5594958504261811 1104 70-71 0.5287227957936622 1104 72-73 0.5924870294233884 1104 74-75 0.532997112555897 1104 76-77 0.4277890003002156 1104 78-79 0.45263140120892587 1104 80-81 0.5563433827461992 1104 82-83 0.604841728056492 1104 84-85 0.5797126355723066 1104 86-87 0.5311181813048194 1104 88-89 0.530390710295201 1104 90-91 0.52955779109001 1104 92-93 0.5979620781223147 1104 94-95 0.6115872516996319 1104 96-97 0.9310620582096156 1104 98-99 0.6688485879922688 1104 100-101 0.7542274748082001 1105 1 0.12542797374178605 1105 2 0.1460546176850528 1105 3 0.12342451101984153 1105 4 0.043325570618129916 1105 5 0.09367562584388622 1105 6 0.050284891227448725 1105 7 0.08221612596233996 1105 8 0.17619615810315992 1105 9 0.1554966906872508 1105 10-11 0.13193782063834902 1105 12-13 0.14508594192444946 1105 14-15 0.16603321423330186 1105 16-17 0.18884888107715625 1105 18-19 0.07106135305343741 1105 20-21 0.16115030622381 1105 22-23 0.1914601388114221 1105 24-25 0.19699514772371884 1105 26-27 0.11995532447649992 1105 28-29 0.21177374588359754 1105 30-31 0.15396137642132857 1105 32-33 0.2219369085977121 1105 34-35 0.18045379484856028 1105 36-37 0.13275643046694796 1105 38-39 0.14821278884159028 1105 40-41 0.1889900422707953 1105 42-43 0.12574753173066 1105 44-45 0.2503050824149966 1105 46-47 0.23202630836248517 1105 48-49 0.2585599337649498 1105 50-51 0.14735923965078257 1105 52-53 0.2395798465088248 1105 54-55 0.2904124987453969 1105 56-57 0.25913964076964646 1105 58-59 0.24342692443423175 1105 60-61 0.23959001310379335 1105 62-63 0.26244045231785407 1105 64-65 0.17180470342741927 1105 66-67 0.2512158722870126 1105 68-69 0.2518651161737253 1105 70-71 0.2791104621930387 1105 72-73 0.2790511597131129 1105 74-75 0.32328117437531745 1105 76-77 0.33048134890628944 1105 78-79 0.2986527723815371 1105 80-81 0.2526858610804723 1105 82-83 0.2918591889700579 1105 84-85 0.31321026581315436 1105 86-87 0.21893237070644034 1105 88-89 0.19903170011728122 1105 90-91 0.24222892454812595 1105 92-93 0.24035344479883491 1105 94-95 0.37145631151718916 1105 96-97 0.6838860994247433 1105 98-99 0.5088651511229116 1105 100-101 0.544250236354408 1106 1 -0.038544479652479424 1106 2 -0.07447277570280164 1106 3 -0.035449117180995415 1106 4 -0.030386444255150025 1106 5 -0.03615116207594582 1106 6 -0.05556206821985654 1106 7 -0.05257075518103704 1106 8 -0.044331800793663945 1106 9 0.0028777319372395027 1106 10-11 -0.03171169208009417 1106 12-13 -0.07157581439508931 1106 14-15 -0.14978840933707716 1106 16-17 -0.13867554875859156 1106 18-19 -0.1403587239629971 1106 20-21 -0.1636875634051833 1106 22-23 -0.12646180144257357 1106 24-25 -0.05405461335971751 1106 26-27 -0.07983591110934185 1106 28-29 -0.07200838261487519 1106 30-31 -0.2757071816204757 1106 32-33 -0.34750220460045966 1106 34-35 -0.4184350183431391 1106 36-37 -0.3110991867170867 1106 38-39 -0.3964893237686056 1106 40-41 -0.32291974261553236 1106 42-43 -0.33921886490827546 1106 44-45 -0.3390305526426758 1106 46-47 -0.3457055592781515 1106 48-49 -0.352816612146718 1106 50-51 -0.3390577909781598 1106 52-53 -0.188789590202596 1106 54-55 -0.18239275193344895 1106 56-57 -0.20068466690261033 1106 58-59 -0.21696842812329464 1106 60-61 -0.3000555028776475 1106 62-63 -0.20281446606441023 1106 64-65 -0.19784939712044292 1106 66-67 -0.1987660204428252 1106 68-69 -0.16608661471907737 1106 70-71 -0.15232973030472152 1106 72-73 -0.12891731879803814 1106 74-75 -0.09522150480290037 1106 76-77 -0.13731746719179228 1106 78-79 -0.08217571536946622 1106 80-81 -0.1718936863864471 1106 82-83 -0.16115619671105463 1106 84-85 -0.15319255165911017 1106 86-87 -0.206312713054146 1106 88-89 -0.2843969006803704 1106 90-91 -0.17916644052070296 1106 92-93 -0.18948532443342003 1106 94-95 -0.16747231434746368 1106 96-97 0.12208859213875911 1106 98-99 -0.12184811651164651 1106 100-101 -0.08562660845679204 1107 1 -0.19878228128305508 1107 2 -0.23714163865129478 1107 3 -0.2019295010677169 1107 4 -0.14817650798338633 1107 5 -0.12335506090243342 1107 6 -0.1464585031571488 1107 7 -0.1646581072527269 1107 8 -0.14282013046298658 1107 9 -0.20218662166367807 1107 10-11 -0.24528996692787786 1107 12-13 -0.18055615166478134 1107 14-15 -0.20319674088184314 1107 16-17 -0.2252190698794223 1107 18-19 -0.2733967489211082 1107 20-21 -0.2764578361434147 1107 22-23 -0.21947949414136048 1107 24-25 -0.15537788251634765 1107 26-27 -0.12993555240843335 1107 28-29 -0.14491669743222957 1107 30-31 -0.24119471817822102 1107 32-33 -0.3354438848090098 1107 34-35 -0.3131958970072972 1107 36-37 -0.2633045311787825 1107 38-39 -0.2560764236494748 1107 40-41 -0.2518166009492546 1107 42-43 -0.31900596987399865 1107 44-45 -0.19680336335030546 1107 46-47 -0.20823705268581705 1107 48-49 -0.3487145764665982 1107 50-51 -0.2726316474232675 1107 52-53 -0.276582827065468 1107 54-55 -0.23940722476143605 1107 56-57 -0.29052690403480597 1107 58-59 -0.25215590079564265 1107 60-61 -0.3560535436476968 1107 62-63 -0.3980366198620402 1107 64-65 -0.4018964339768587 1107 66-67 -0.32567888598196504 1107 68-69 -0.3769220060186811 1107 70-71 -0.33110697283756707 1107 72-73 -0.3312583115226104 1107 74-75 -0.31554771208082144 1107 76-77 -0.3881365435474322 1107 78-79 -0.1925612724522736 1107 80-81 -0.24864598638840363 1107 82-83 -0.26063029224567913 1107 84-85 -0.21949708059510442 1107 86-87 -0.224296950260495 1107 88-89 -0.2719211809141626 1107 90-91 -0.27757757080781786 1107 92-93 -0.29064448533543796 1107 94-95 -0.26324428341592565 1107 96-97 0.03768510575535444 1107 98-99 -0.23938718517487345 1107 100-101 -0.3651132623929705 1108 1 -0.3123172123046771 1108 2 -0.3327820110689501 1108 3 -0.338850967464829 1108 4 -0.23172944893751435 1108 5 -0.24857434427658376 1108 6 -0.22368229434712816 1108 7 -0.2612431925370089 1108 8 -0.22095339875244235 1108 9 -0.3375045436465953 1108 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-0.18879653732414425 2305 20-21 -0.17665152783661853 2305 22-23 -0.18427343376392002 2305 24-25 -0.10395454524130088 2305 26-27 -0.19795099125058613 2305 28-29 -0.19556790470399932 2305 30-31 -0.23501523818300996 2305 32-33 -0.2393032249003113 2305 34-35 -0.2122526120765471 2305 36-37 -0.1397614554299551 2305 38-39 -0.19512652978635003 2305 40-41 -0.15805543630961694 2305 42-43 -0.22790012832437867 2305 44-45 -0.17189918200553933 2305 46-47 -0.09187275340183731 2305 48-49 -0.1551565917941673 2305 50-51 -0.15454134605467118 2305 52-53 -0.21467014276676366 2305 54-55 -0.15729403626893657 2305 56-57 -0.20701355174023917 2305 58-59 -0.13259033088261418 2305 60-61 -0.16123750556337058 2305 62-63 -0.1544574564359067 2305 64-65 -0.17297505544490122 2305 66-67 -0.17840267157728107 2305 68-69 -0.20674317747741355 2305 70-71 -0.15992716032977938 2305 72-73 -0.14367457783843918 2305 74-75 -0.2708840509574131 2305 76-77 -0.2551546476907234 2305 78-79 -0.25607867723274325 2305 80-81 -0.18564508477528818 2305 82-83 -0.12299739674610066 2305 84-85 -0.09742336047288802 2305 86-87 -0.08402771712390233 2305 88-89 -0.12763400880415432 2305 90-91 -0.1056193235590861 2305 92-93 -0.060741633684777696 2305 94-95 -0.18814961706727473 2305 96-97 0.10216827969280828 2305 98-99 -0.16201163303836452 2305 100-101 -0.1373323289811914 2306 1 -0.2186156963127992 2306 2 -0.284155020780851 2306 3 -0.24655388751880025 2306 4 -0.1811903102458743 2306 5 -0.21947746907134302 2306 6 -0.14693175292642735 2306 7 -0.22648825532566974 2306 8 -0.19069069839478203 2306 9 -0.3448537974294439 2306 10-11 -0.2596238577572123 2306 12-13 -0.2693583996966993 2306 14-15 -0.37184066307639085 2306 16-17 -0.41547528648084864 2306 18-19 -0.39881490377765516 2306 20-21 -0.4420519216235945 2306 22-23 -0.3683295453944595 2306 24-25 -0.368357939029778 2306 26-27 -0.39201079496843505 2306 28-29 -0.46919995185058383 2306 30-31 -0.4879401424505332 2306 32-33 -0.5549600148277349 2306 34-35 -0.5578652610253272 2306 36-37 -0.5221078005399491 2306 38-39 -0.67370935605733 2306 40-41 -0.5109353161226622 2306 42-43 -0.6313004126844675 2306 44-45 -0.6228644132539998 2306 46-47 -0.5664154877742149 2306 48-49 -0.5315559041168214 2306 50-51 -0.5182538899065605 2306 52-53 -0.4581038877492034 2306 54-55 -0.4807031573492111 2306 56-57 -0.4904665635939338 2306 58-59 -0.49936160668950436 2306 60-61 -0.5517824806901572 2306 62-63 -0.4670090935840676 2306 64-65 -0.5422266531380302 2306 66-67 -0.5088929033019056 2306 68-69 -0.4801790640951822 2306 70-71 -0.5087550042000188 2306 72-73 -0.506404638230812 2306 74-75 -0.45478751155049935 2306 76-77 -0.41596779643646187 2306 78-79 -0.4009654216567 2306 80-81 -0.39638841324861573 2306 82-83 -0.4046490425399796 2306 84-85 -0.4106341849174271 2306 86-87 -0.40782517791373607 2306 88-89 -0.3791080171016219 2306 90-91 -0.39251842177171525 2306 92-93 -0.4319243234004233 2306 94-95 -0.4741047969500194 2306 96-97 -0.16107024209409104 2306 98-99 -0.426989045375052 2306 100-101 -0.4026936226309452 2307 1 -0.4836292030100502 2307 2 -0.49396381333530925 2307 3 -0.506882384577132 2307 4 -0.4077862352884196 2307 5 -0.39910273745203284 2307 6 -0.41824477843167074 2307 7 -0.42677986175014837 2307 8 -0.42687156147207617 2307 9 -0.5058423333079176 2307 10-11 -0.6102036433751366 2307 12-13 -0.5847309636598439 2307 14-15 -0.8754506783562377 2307 16-17 -0.8151314350227139 2307 18-19 -0.7398713217686819 2307 20-21 -0.6151473366462668 2307 22-23 -0.6217125487842807 2307 24-25 -0.619017406925849 2307 26-27 -0.7319883688686915 2307 28-29 -0.7121854160057737 2307 30-31 -0.6904599803330669 2307 32-33 -0.7832406339070985 2307 34-35 -0.7488476216474211 2307 36-37 -0.7820913312780746 2307 38-39 -0.7940539256822916 2307 40-41 -0.7079126240077258 2307 42-43 -0.8435868090768679 2307 44-45 -0.6799961900847293 2307 46-47 -0.7045477617221039 2307 48-49 -0.6621133692180123 2307 50-51 -0.7017005166696038 2307 52-53 -0.8365301953499937 2307 54-55 -0.8492533725074694 2307 56-57 -0.8472289087010623 2307 58-59 -0.8238106911077807 2307 60-61 -0.8986492274852154 2307 62-63 -0.9102934925686696 2307 64-65 -0.8658118501716032 2307 66-67 -0.8155009901233896 2307 68-69 -0.7908821311937189 2307 70-71 -0.8766834729297202 2307 72-73 -1.1873922815449731 2307 74-75 -0.9531748112915039 2307 76-77 -0.8350082295993317 2307 78-79 -0.8555303061733497 2307 80-81 -0.7820909035198795 2307 82-83 -0.7271183739664835 2307 84-85 -0.6959742876729607 2307 86-87 -0.6450016330524448 2307 88-89 -0.7370789249700636 2307 90-91 -0.8435394659147555 2307 92-93 -0.7866485370541412 2307 94-95 -0.8304473020393388 2307 96-97 -0.5989909106686042 2307 98-99 -0.9882616366379828 2307 100-101 -1.0363346637967474 2308 1 -1.4572095442374504 2308 2 -0.894297242161386 2308 3 -0.8880894128429837 2308 4 -0.5411438396938166 2308 5 -0.6625711930160776 2308 6 -0.6615105506496448 2308 7 -0.6139217639655783 2308 8 -0.6143357516936163 2308 9 -0.8740066444869328 2308 10-11 -0.8352581538156798 2308 12-13 -0.7656141123738749 2308 14-15 -0.965577922435692 2308 16-17 -0.9318673339277268 2308 18-19 -0.9661284439357374 2308 20-21 -0.9380620138807885 2308 22-23 -1.0548407716643453 2308 24-25 -1.1994222961542746 2308 26-27 -1.1383895657110727 2308 28-29 -1.1625530630645997 2308 30-31 -1.0552561829049978 2308 32-33 -1.1063373441821156 2308 34-35 -1.1958014031600186 2308 36-37 -1.2880486858108 2308 38-39 -1.2540146145032764 2308 40-41 -1.2057232539415068 2308 42-43 -1.0724938548497178 2308 44-45 -1.1076773477750734 2308 46-47 -1.0919270031914152 2308 48-49 -1.1849659761698845 2308 50-51 -1.2962820993026583 2308 52-53 -1.22451820305308 2308 54-55 -1.177236128109712 2308 56-57 -1.093144604524845 2308 58-59 -1.0487468303361922 2308 60-61 -0.9790013967620155 2308 62-63 -1.022319410890546 2308 64-65 -1.0096089997132367 2308 66-67 -0.9671743014933938 2308 68-69 -1.0541864586065017 2308 70-71 -1.0156204397619106 2308 72-73 -1.0670515316086338 2308 74-75 -1.1735123781364223 2308 76-77 -1.1274308804778634 2308 78-79 -1.4767643111389717 2308 80-81 -1.152991678602639 2308 82-83 -1.0873896131439693 2308 84-85 -1.0109531201150297 2308 86-87 -1.0151036511324065 2308 88-89 -1.0046287005621188 2308 90-91 -1.0586438315246305 2308 92-93 -0.9603697300768061 2308 94-95 -1.0928752845959977 2308 96-97 -0.9424713426141089 2308 98-99 -1.3569650839259886 2308 100-101 -1.3847618187496344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31.0 3 68.0 4 122.0 5 319.0 6 600.0 7 1301.0 8 2805.0 9 4361.0 10 6078.0 11 7438.0 12 8921.0 13 9922.0 14 11133.0 15 12306.0 16 14232.0 17 16317.0 18 19043.0 19 21609.0 20 23694.0 21 24064.0 22 26177.0 23 30110.0 24 36694.0 25 45734.0 26 58701.0 27 75462.0 28 96215.0 29 120462.0 30 150442.0 31 185484.0 32 228643.0 33 286809.0 34 376087.0 35 534683.0 36 754131.0 37 1293217.0 38 1727159.0 39 601552.0 40 2391.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.600414579435522 31.50612916538737 29.05954105081974 21.83391520435737 2 22.081441375645085 29.905717798607395 28.552652185023074 19.460188640724446 3 21.09664609180322 28.737580971333138 28.895752068492868 21.27002086837077 4 21.18222545585603 29.027995797776118 28.98634922922298 20.80342951714487 5 21.624732260202286 29.01683070039968 28.960773368129967 20.397663671268063 6 21.46781642778204 28.972897125357527 28.499598724311642 21.05968772254879 7 21.648621611773805 28.99487960775503 28.843511579764204 20.512987200706963 8 21.078336627316446 28.96147147780454 28.789896710258194 21.17029518462082 9 20.78464482934582 28.791047709144767 29.337951797592215 21.0863556639172 10-11 20.825056273247245 29.26778229476865 28.811000104629574 21.09616132735453 12-13 20.842937217707433 29.22191697518694 28.736446031318142 21.198699775787485 14-15 21.00730690084125 29.291276256262915 28.54783398441885 21.153582858476984 16-17 21.15123492978299 29.043995341122493 28.55383587714287 21.25093385195165 18-19 21.165667353973877 29.07637474526808 28.49668582527566 21.261272075482385 20-21 21.233089593877306 28.98596481599503 28.57187824170077 21.209067348426895 22-23 21.219546322446245 28.949823901485498 28.576201999571648 21.254427776496605 24-25 21.2045941082268 28.92031119011324 28.518845859634258 21.356248842025707 26-27 21.389935160779444 28.84490091258325 28.625169751402566 21.13999417523474 28-29 21.372329102708232 28.850489330351657 28.54287398505279 21.234307581887315 30-31 21.326162954762605 28.88807086400988 28.51352487637789 21.272241304849633 32-33 21.412075133131225 28.851061637970822 28.448457902445618 21.288405326452338 34-35 21.406814305401248 28.87680814355588 28.461488906697273 21.25488864434559 36-37 21.447235365323763 28.8869629351574 28.396942879443987 21.268858820074847 38-39 21.45958400279873 28.84560270375729 28.392401104876548 21.302412188567434 40-41 21.46155773035712 28.884218793496295 28.371093651978562 21.28312982416802 42-43 21.50812009126986 28.885847669027754 28.317047268353722 21.288984971348665 44-45 21.478837018089468 28.88273666350821 28.345501229214044 21.292925089188273 46-47 21.498116777340957 28.88889166074976 28.328301549831387 21.284690012077895 48-49 21.511370505055606 28.898284353828746 28.26688230435114 21.323462836764513 50-51 21.51732103683944 28.920024706079683 28.251180531210064 21.311473725870815 52-53 21.52687417171312 28.929445769964328 28.21281390889479 21.330866149427756 54-55 21.54793215718737 28.94095062056489 28.13939711354451 21.37172010870323 56-57 21.536984939651628 28.962133339751006 28.108778655919416 21.39210306467795 58-59 21.559510380559622 28.996031560270524 28.088461735439207 21.355996323730647 60-61 21.525032614230515 28.97615529216334 28.13055524168282 21.368256851923324 62-63 21.52767459717375 28.984398358942297 28.09651321096939 21.39141383291456 64-65 21.530259110985952 28.999794995753902 28.121108936497553 21.348836956762593 66-67 21.56436275588818 29.02816787732042 28.05849291950987 21.34897644728153 68-69 21.533768484508744 29.056532477118612 28.067082056030607 21.34261698234204 70-71 21.59100114050787 29.06584745369732 28.006504651546376 21.336646754248438 72-73 21.544896672015053 29.04312031643049 28.03626935097646 21.375713660577997 74-75 21.592246310850477 29.088771006459595 28.028284054424446 21.290698628265474 76-77 21.598583743747366 29.10677102522609 27.995797133061128 21.298848097965415 78-79 21.61257233757422 29.08184488216389 27.980180315898608 21.32540246436329 80-81 21.590026282153815 29.120597078880213 27.96790231432634 21.32147432463963 82-83 21.545334030979205 29.165588976960443 27.94966148986722 21.33941550219313 84-85 21.57178164512214 29.194963570072037 27.920350868516103 21.31290391628972 86-87 21.570787789467836 29.22281099824435 27.903093374679315 21.3033078376085 88-89 21.597943598100834 29.27395523057006 27.864311860680758 21.263789310648352 90-91 21.56650058214335 29.294244143955332 27.86639097282459 21.272864301076723 92-93 21.5513828858457 29.31948876178142 27.844348800725893 21.284779551646984 94-95 21.550890205524485 29.382229180441477 27.822682745138454 21.244197868895583 96-97 21.543249905433296 29.38405342051469 27.800509520135897 21.272187153916125 98-99 21.585110138835205 29.396036905233252 27.731096818080886 21.287756137850657 100-101 21.597899895693782 29.416012935557024 27.692383149648364 21.29370401910083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 55258.0 1 30841.5 2 4864.5 3 2939.0 4 2407.0 5 2173.5 6 2159.0 7 2166.0 8 2047.5 9 1873.5 10 1903.5 11 2341.5 12 4374.0 13 4320.5 14 2656.5 15 3009.5 16 3628.0 17 4563.5 18 5910.5 19 7756.5 20 10654.5 21 14833.5 22 20047.5 23 27682.0 24 35975.0 25 45615.5 26 64632.0 27 84599.5 28 93057.0 29 98823.0 30 110073.5 31 125478.5 32 143363.0 33 161473.5 34 179800.5 35 194115.5 36 205593.0 37 216234.5 38 221281.5 39 222649.5 40 227438.5 41 235237.0 42 239970.0 43 243312.0 44 245634.5 45 247311.0 46 248640.0 47 248542.0 48 245377.5 49 239348.5 50 233730.5 51 223604.0 52 211734.5 53 200107.0 54 186062.0 55 169677.5 56 151942.5 57 134636.5 58 116775.0 59 97545.5 60 76091.0 61 56921.0 62 41718.0 63 29918.0 64 22333.5 65 16120.5 66 10349.5 67 6972.0 68 4890.0 69 3293.5 70 1898.0 71 858.5 72 392.5 73 205.5 74 117.5 75 84.0 76 53.5 77 38.0 78 24.0 79 18.0 80 16.0 81 9.0 82 4.5 83 3.0 84 5.0 85 4.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012693489501897201 2 0.018313843813141856 3 0.005532306985219936 4 4.402366301236023E-4 5 9.538460319344717E-4 6 0.0 7 0.0 8 0.008481892407048072 9 0.010389584470917014 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.1005915753090058E-4 24-25 0.005202129512627234 26-27 0.007462010880595059 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 2.2011831506180116E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 1.0272188036217388E-4 62-63 1.394082662058074E-4 64-65 3.5218930409888186E-4 66-67 2.4946742373670796E-4 68-69 3.962129671112421E-4 70-71 1.320709890370807E-4 72-73 4.402366301236023E-5 74-75 8.804732602472046E-5 76-77 0.12747051625228906 78-79 0.05743620567679264 80-81 1.0272188036217388E-4 82-83 0.0025166860688732597 84-85 0.010015383335311951 86-87 0.12051477749633613 88-89 0.13833702373917328 90-91 0.15189631194698025 92-93 0.14987856072558042 94-95 0.11531998526087762 96-97 1.2105993718997252 98-99 0.0776724161081409 100-101 0.06985087864627823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6814517.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.57135306985408 #Duplication Level Percentage of deduplicated Percentage of total 1 89.93985896405584 68.86816695789601 2 6.125300929347133 9.380451602402893 3 1.427214339013925 3.27851199176981 4 0.6402613280398711 1.9610270482525856 5 0.39640864562773037 1.5176773182151806 6 0.27663812392317033 1.2709533275701874 7 0.20980313836798783 1.1245437128197053 8 0.15985487596110812 0.9792243317724593 9 0.12765885630310092 0.8797510222630658 >10 0.6726935027387669 8.378400724675902 >50 0.022126200414038892 1.152228902085619 >100 0.0019291450690644895 0.218830987914372 >500 0.0 0.0 >1k 1.5117068295879017E-4 0.36091921777106006 >5k 1.0078045530586009E-4 0.6293128545911898 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9102 0.13356779357950094 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 7208 0.10577418766436418 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.934910867490682E-5 0.0 2 0.0 0.0 0.0 2.934910867490682E-5 0.0 3 0.0 2.934910867490682E-5 0.0 2.934910867490682E-5 0.0 4 0.0 2.934910867490682E-5 0.0 4.402366301236023E-5 0.0 5 0.0 2.934910867490682E-5 0.0 4.402366301236023E-5 0.0 6 0.0 2.934910867490682E-5 0.0 4.402366301236023E-5 0.0 7 0.0 2.934910867490682E-5 0.0 5.869821734981364E-5 0.0 8 0.0 2.934910867490682E-5 0.0 5.869821734981364E-5 0.0 9 0.0 4.402366301236023E-5 0.0 5.869821734981364E-5 0.0 10-11 0.0 5.136094018108694E-5 0.0 7.337277168726705E-5 0.0 12-13 0.0 5.869821734981364E-5 0.0 7.337277168726705E-5 1.467455433745341E-5 14-15 0.0 7.337277168726705E-5 0.0 7.337277168726705E-5 2.934910867490682E-5 16-17 0.0 7.337277168726705E-5 0.0 9.538460319344717E-5 4.402366301236023E-5 18-19 1.467455433745341E-5 7.337277168726705E-5 0.0 1.0272188036217388E-4 4.402366301236023E-5 20-21 1.467455433745341E-5 7.337277168726705E-5 0.0 1.0272188036217388E-4 4.402366301236023E-5 22-23 1.467455433745341E-5 7.337277168726705E-5 0.0 1.1005915753090058E-4 4.402366301236023E-5 24-25 2.2011831506180116E-5 7.337277168726705E-5 0.0 1.1739643469962729E-4 4.402366301236023E-5 26-27 4.402366301236023E-5 7.337277168726705E-5 0.0 1.1739643469962729E-4 4.402366301236023E-5 28-29 4.402366301236023E-5 8.071004885599376E-5 0.0 1.1739643469962729E-4 4.402366301236023E-5 30-31 4.402366301236023E-5 8.804732602472046E-5 0.0 1.1739643469962729E-4 4.402366301236023E-5 32-33 4.402366301236023E-5 9.538460319344717E-5 0.0 1.320709890370807E-4 4.402366301236023E-5 34-35 4.402366301236023E-5 1.0272188036217388E-4 0.0 1.320709890370807E-4 4.402366301236023E-5 36-37 4.402366301236023E-5 1.0272188036217388E-4 0.0 1.394082662058074E-4 4.402366301236023E-5 38-39 4.402366301236023E-5 1.0272188036217388E-4 0.0 1.7609465204944093E-4 4.402366301236023E-5 40-41 4.402366301236023E-5 1.0272188036217388E-4 0.0 1.9810648355562103E-4 4.402366301236023E-5 42-43 4.402366301236023E-5 1.1005915753090058E-4 0.0 2.2011831506180116E-4 4.402366301236023E-5 44-45 7.337277168726705E-5 1.1739643469962729E-4 0.0 2.4213014656798126E-4 4.402366301236023E-5 46-47 8.071004885599376E-5 1.1739643469962729E-4 0.0 2.788165324116148E-4 4.402366301236023E-5 48-49 8.804732602472046E-5 1.24733711868354E-4 0.0 2.788165324116148E-4 5.869821734981364E-5 50-51 9.538460319344717E-5 1.5408282054326083E-4 0.0 2.934910867490682E-4 5.869821734981364E-5 52-53 1.320709890370807E-4 1.6142009771198752E-4 0.0 2.934910867490682E-4 5.869821734981364E-5 54-55 1.6142009771198752E-4 1.6142009771198752E-4 0.0 3.081656410865216E-4 5.869821734981364E-5 56-57 1.8343192921816765E-4 1.6142009771198752E-4 0.0 3.081656410865216E-4 5.869821734981364E-5 58-59 2.1278103789307447E-4 1.6142009771198752E-4 0.0 3.081656410865216E-4 5.869821734981364E-5 60-61 2.6414197807416134E-4 1.6142009771198752E-4 0.0 3.081656410865216E-4 5.869821734981364E-5 62-63 3.0082836391779493E-4 1.7609465204944093E-4 0.0 3.081656410865216E-4 5.869821734981364E-5 64-65 3.375147497614284E-4 1.7609465204944093E-4 0.0 3.081656410865216E-4 5.869821734981364E-5 66-67 3.668638584363353E-4 1.7609465204944093E-4 0.0 3.081656410865216E-4 7.337277168726705E-5 68-69 4.255620757861489E-4 1.7609465204944093E-4 0.0 3.301774725927017E-4 7.337277168726705E-5 70-71 5.136094018108693E-4 1.7609465204944093E-4 0.0 3.375147497614284E-4 7.337277168726705E-5 72-73 6.310058365104966E-4 1.7609465204944093E-4 0.0 3.4485202693015514E-4 7.337277168726705E-5 74-75 7.777513798850307E-4 1.9810648355562103E-4 0.0 3.595265812676086E-4 7.337277168726705E-5 76-77 9.391714775970182E-4 2.1278103789307447E-4 0.0 3.6686385843633524E-4 8.071004885599376E-5 78-79 0.001122603406815186 2.2011831506180116E-4 0.0 3.6686385843633524E-4 8.804732602472046E-5 80-81 0.0013647335533831671 2.2745559223052785E-4 0.0 4.035502442799688E-4 1.0272188036217388E-4 82-83 0.0017536092433256827 2.3479286939925457E-4 0.0 4.108875214486955E-4 1.0272188036217388E-4 84-85 0.002230532259292918 2.3479286939925457E-4 0.0 4.328993529548756E-4 1.0272188036217388E-4 86-87 0.002861538095803415 2.6414197807416134E-4 0.0 4.402366301236023E-4 1.0272188036217388E-4 88-89 0.0037640231875568 2.788165324116148E-4 0.0 4.47573907292329E-4 1.0272188036217388E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 290 0.0 27.796644 3 CGCGTAG 150 0.0 26.864471 54-55 ACTCGCG 190 0.0 22.45629 50-51 CTCGCGT 210 0.0 20.317595 52-53 CGTAGCG 210 0.0 19.18898 56-57 GCGACGA 235 0.0 19.164963 60-61 CAACGCA 5755 0.0 18.164034 12-13 ATCAACG 5865 0.0 17.702114 10-11 CGTACGC 230 0.0 17.522429 20-21 ACGTACG 230 0.0 17.522429 20-21 TCGCGTA 260 0.0 17.32205 52-53 ACGCAGA 6310 0.0 16.52884 14-15 GTATCAA 7720 0.0 15.967619 9 GGTATCA 7785 0.0 15.774324 8 GTGGTAT 7695 0.0 15.773968 6 AGTGGTA 7775 0.0 15.611777 5 AAGCAGT 8420 0.0 15.43333 1 TGGTATC 8025 0.0 15.302569 7 TCAACGC 5590 0.0 14.714203 10-11 AACGCAG 6185 0.0 13.528637 12-13 >>END_MODULE