FastQCFastQC Report
Sat 18 Jun 2016
SRR578580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10735971
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130563512.161312656302815No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT292850.2727745818240381No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG108690.10123909611901895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2874300.091.727665
AAGCAGT2897900.091.401091
GTATCAA2887400.091.274029
TGGTATC2888400.091.231247
GGTATCA2892050.091.179588
GTGGTAT2912250.090.626646
AGCAGTG2926400.090.4867862
GCAGTGG2926250.090.38873
CAGTGGT2921500.090.187644
TCAACGC2880400.046.3509412-13
AACGCAG2890350.046.27712214-15
CAACGCA2879950.046.20723712-13
ATCAACG2877900.046.17745210-11
CGCAGAG2904800.046.10283316-17
ACGCAGA2898550.045.97001314-15
TATCAAC2895550.045.9698210-11
CAGAGTA2886350.045.82576418-19
AGAGTAC2838900.045.6564218-19
GAGTACT1663450.045.5517720-21
GCAGAGT2916900.045.45805416-17
Produced by FastQC (version 0.11.5)