FastQCFastQC Report
Sat 18 Jun 2016
SRR578580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10735971
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237790.22148904835901664No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107480.10011204389430634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4850.031.3402043
CGCGTAG2850.026.66660554-55
TCGCGTA3950.020.44384852-53
CAACGCA75100.020.27026612-13
CGTACGC3550.020.06953420-21
CTCGCGT3700.019.89943752-53
ATCAACG77450.019.65522210-11
ACTCGCG3750.019.63461350-51
ACGTACG4000.018.99915920-21
AGTGGTA98550.017.3029165
TCAACGC76000.016.65539610-11
GTATCAA104800.016.3159589
CGTAGCG5000.016.14947356-57
GGTATCA106000.015.9520138
AACGCAG80850.015.68565312-13
TGGTATC112350.015.388627
ATCGGAA20000.014.96252
CGCTAGT5100.014.90140690-91
AAGCAGT117600.014.6832591
ACGCAGA106650.014.38517114-15
Produced by FastQC (version 0.11.5)