##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578580_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10735971 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47824067334012 31.0 31.0 34.0 30.0 34.0 2 31.792131144914606 31.0 31.0 34.0 30.0 34.0 3 31.863771334702747 31.0 31.0 34.0 30.0 34.0 4 35.537572987110345 37.0 35.0 37.0 33.0 37.0 5 35.35826428741285 37.0 35.0 37.0 33.0 37.0 6 35.35592448973642 37.0 35.0 37.0 32.0 37.0 7 35.334180764832546 37.0 35.0 37.0 32.0 37.0 8 35.319544175370815 37.0 35.0 37.0 32.0 37.0 9 36.885321690976994 39.0 37.0 39.0 33.0 39.0 10-11 36.851390060573 39.0 37.0 39.0 32.5 39.0 12-13 36.82912262896389 39.0 37.0 39.0 32.0 39.0 14-15 38.01062111661814 40.0 37.0 41.0 33.0 41.0 16-17 37.95950738875878 40.0 37.0 41.0 33.0 41.0 18-19 37.89749744107915 40.0 37.0 41.0 32.5 41.0 20-21 37.845556261282745 40.0 37.0 41.0 32.0 41.0 22-23 37.75540884937189 40.0 37.0 41.0 32.0 41.0 24-25 37.66666238200531 39.5 36.5 41.0 32.0 41.0 26-27 37.47889683196797 39.0 36.0 41.0 32.0 41.0 28-29 37.306464082289345 39.0 36.0 41.0 31.0 41.0 30-31 37.178883353913676 39.0 36.0 41.0 31.0 41.0 32-33 37.04923727905003 39.0 36.0 40.5 31.0 41.0 34-35 36.89274640365552 39.0 36.0 40.0 30.0 41.0 36-37 36.74432419759703 39.0 35.0 40.0 30.0 41.0 38-39 36.58044502914548 38.0 35.0 40.0 30.0 41.0 40-41 36.4134269736757 38.0 35.0 40.0 30.0 41.0 42-43 36.352289373732475 38.0 35.0 40.0 30.0 41.0 44-45 36.386603689596406 38.0 35.0 40.0 30.0 41.0 46-47 36.25765280103681 38.0 35.0 40.0 30.0 41.0 48-49 36.11165976510182 38.0 35.0 40.0 29.0 41.0 50-51 35.911719116976 38.0 34.5 40.0 28.5 41.0 52-53 35.9712272415788 38.0 35.0 40.0 28.5 41.0 54-55 35.95931094635036 38.0 35.0 40.0 28.5 41.0 56-57 35.79454718161962 38.0 34.0 40.0 28.0 41.0 58-59 35.58255820549441 38.0 34.0 40.0 28.0 41.0 60-61 35.31926981732719 38.0 34.0 40.0 28.0 41.0 62-63 35.03414944954676 37.0 34.0 40.0 27.5 41.0 64-65 34.70552398101671 37.0 33.0 40.0 26.5 41.0 66-67 34.35888211695058 36.0 33.0 39.0 26.0 41.0 68-69 33.988905102295824 36.0 33.0 39.0 26.0 41.0 70-71 33.60534137992735 35.5 32.0 39.0 26.0 40.0 72-73 33.21138106650996 35.0 32.0 38.0 26.0 40.0 74-75 32.80117746219695 35.0 31.5 37.0 25.0 39.5 76-77 31.447730531313844 34.0 29.5 36.0 23.0 39.0 78-79 32.09397072700737 34.5 31.0 36.0 25.0 39.0 80-81 31.974441249887878 35.0 31.0 36.0 24.5 38.5 82-83 31.669106781305576 34.5 31.0 36.0 24.0 37.0 84-85 31.34752762465547 34.0 31.0 35.0 23.5 37.0 86-87 31.002417992746068 34.0 31.0 35.0 22.0 36.5 88-89 30.682200566674407 34.0 31.0 35.0 20.0 36.0 90-91 30.356026343588297 34.0 30.0 35.0 19.5 36.0 92-93 29.99362908115158 34.0 30.0 35.0 18.0 35.5 94-95 29.624609222584525 34.0 30.0 35.0 13.0 35.0 96-97 29.236214591116166 34.0 29.0 35.0 4.5 35.0 98-99 28.754847558735023 34.0 29.0 35.0 2.0 35.0 100-101 27.630005613837817 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2983967336521971 1101 2 0.323049533060324 1101 3 0.30698314713027486 1101 4 0.21326040605779895 1101 5 0.27937610884971065 1101 6 0.24639047399913494 1101 7 0.29963056269516386 1101 8 0.23868153457815566 1101 9 0.2914300089010311 1101 10-11 0.29645459342358293 1101 12-13 0.311649339315494 1101 14-15 0.38999116025431846 1101 16-17 0.3956752204104177 1101 18-19 0.32231975237353794 1101 20-21 0.35347730270753175 1101 22-23 0.43726539533248854 1101 24-25 0.3819705259224051 1101 26-27 0.3591157604662101 1101 28-29 0.4686272601187227 1101 30-31 0.4903202220489291 1101 32-33 0.4221327606375027 1101 34-35 0.39174670230752184 1101 36-37 0.47261476131959057 1101 38-39 0.4497616553623942 1101 40-41 0.4931238610345403 1101 42-43 0.4990582409833735 1101 44-45 0.4518279734869708 1101 46-47 0.4061217558314212 1101 48-49 0.3821542605094592 1101 50-51 0.3770622849853993 1101 52-53 0.4726492945268319 1101 54-55 0.44500850721284735 1101 56-57 0.5147347003522853 1101 58-59 0.4813246776432223 1101 60-61 0.4249372162251319 1101 62-63 0.4091601401300764 1101 64-65 0.37642371849628375 1101 66-67 0.3898752427100405 1101 68-69 0.44518727553735715 1101 70-71 0.40951854874460736 1101 72-73 0.430998055331294 1101 74-75 0.3936519645465779 1101 76-77 0.27099316573766785 1101 78-79 0.3617197883931098 1101 80-81 0.35550378852883924 1101 82-83 0.3984960879423092 1101 84-85 0.4758492101356815 1101 86-87 0.4271903024101462 1101 88-89 0.3445417177579593 1101 90-91 0.4677678784016166 1101 92-93 0.47150722783448984 1101 94-95 0.4710773833264952 1101 96-97 0.38037590526998954 1101 98-99 0.4311788224763369 1101 100-101 0.6564976032569412 1102 1 0.2593307821830386 1102 2 0.2092017761132503 1102 3 0.2736362628864626 1102 4 0.19893270557280118 1102 5 0.22984094498524854 1102 6 0.13595191599870304 1102 7 0.17930280805639853 1102 8 0.14446334627581336 1102 9 0.3235204978687918 1102 10-11 0.22801019476717954 1102 12-13 0.15974524777449517 1102 14-15 0.32428365892514677 1102 16-17 0.3209123500423132 1102 18-19 0.3417418157842249 1102 20-21 0.35731514409192044 1102 22-23 0.26842074521184145 1102 24-25 0.24631677103027272 1102 26-27 0.3026047833635701 1102 28-29 0.23972828882035202 1102 30-31 0.2562428980564775 1102 32-33 0.29498747325617813 1102 34-35 0.3208709307976676 1102 36-37 0.2994060149917388 1102 38-39 0.30330630651654644 1102 40-41 0.30251502079341464 1102 42-43 0.2994721898881352 1102 44-45 0.25113514207450294 1102 46-47 0.1845930971522307 1102 48-49 0.23038765311108023 1102 50-51 0.1459918180841271 1102 52-53 0.2353258454860594 1102 54-55 0.2783370268786811 1102 56-57 0.3525373259865532 1102 58-59 0.3642292243470209 1102 60-61 0.293169358333202 1102 62-63 0.20441145402514138 1102 64-65 0.23778551625517963 1102 66-67 0.2492188308698573 1102 68-69 0.17029627400753355 1102 70-71 0.24074608960696509 1102 72-73 0.30445949582035325 1102 74-75 0.19521369723679527 1102 76-77 0.14599730092434626 1102 78-79 0.21371138174682613 1102 80-81 0.2973707286267846 1102 82-83 0.2062309470211794 1102 84-85 0.15125492958637565 1102 86-87 0.12573445949308493 1102 88-89 0.260141680851099 1102 90-91 0.35724379206742896 1102 92-93 0.2925778944219033 1102 94-95 0.16137815975952563 1102 96-97 0.11696690834773094 1102 98-99 0.1916592654634428 1102 100-101 0.3808475137980203 1103 1 0.19763741944014157 1103 2 0.24224678517341047 1103 3 0.28179899088554805 1103 4 0.1734568675708985 1103 5 0.23859002791914463 1103 6 0.17120380455776996 1103 7 0.143128312327228 1103 8 0.14670459773737576 1103 9 0.29412998066867146 1103 10-11 0.2700826346851031 1103 12-13 0.2699278090183128 1103 14-15 0.41978663302418795 1103 16-17 0.3703756450232092 1103 18-19 0.34452807241667927 1103 20-21 0.32226485679410644 1103 22-23 0.33504692385398016 1103 24-25 0.3522397620938378 1103 26-27 0.31882748037114794 1103 28-29 0.31565567521236915 1103 30-31 0.41687423487233843 1103 32-33 0.4001753609645462 1103 34-35 0.43714081146857353 1103 36-37 0.4713740642574962 1103 38-39 0.510851217704456 1103 40-41 0.4375517048104811 1103 42-43 0.4216526068193076 1103 44-45 0.3627786993310451 1103 46-47 0.34131733655131313 1103 48-49 0.31934346381673606 1103 50-51 0.36518912202644316 1103 52-53 0.3333392586045605 1103 54-55 0.33154784954452055 1103 56-57 0.32005027564178334 1103 58-59 0.42548055596316914 1103 60-61 0.46498918537341183 1103 62-63 0.34285846634112005 1103 64-65 0.38313519835570986 1103 66-67 0.37971899234303663 1103 68-69 0.3824093617912112 1103 70-71 0.4158732251458801 1103 72-73 0.44476349093873324 1103 74-75 0.4424212498075448 1103 76-77 0.3732057887492495 1103 78-79 0.4704809562638417 1103 80-81 0.5566893831676047 1103 82-83 0.4869596463562793 1103 84-85 0.5210402905894433 1103 86-87 0.5334665999594748 1103 88-89 0.4973104148839411 1103 90-91 0.5290515440797563 1103 92-93 0.46186500838591726 1103 94-95 0.47845192431579164 1103 96-97 0.48697729772185383 1103 98-99 0.5609053819853109 1103 100-101 0.6574423303120476 1104 1 0.17449928668741066 1104 2 0.15125243426151513 1104 3 0.21127433711321686 1104 4 0.15876123180108692 1104 5 0.1712061516681871 1104 6 0.20597790415131811 1104 7 0.08338122318377827 1104 8 0.1369300854504587 1104 9 0.2304871543785083 1104 10-11 0.23212961764055962 1104 12-13 0.19729541323310684 1104 14-15 0.19758326179162822 1104 16-17 0.21437670328376868 1104 18-19 0.2330006196563872 1104 20-21 0.26283226206862054 1104 22-23 0.25390885589631296 1104 24-25 0.2529307343844138 1104 26-27 0.24049822556649758 1104 28-29 0.26340211340374964 1104 30-31 0.18979241309283879 1104 32-33 0.19418739642740235 1104 34-35 0.22907787762742515 1104 36-37 0.29967797483394776 1104 38-39 0.3375283528056201 1104 40-41 0.34971077705106524 1104 42-43 0.20098642791059973 1104 44-45 0.2767952845727919 1104 46-47 0.2574452390105435 1104 48-49 0.21953837867522452 1104 50-51 0.27328602913900824 1104 52-53 0.2783629445557665 1104 54-55 0.21435104918491987 1104 56-57 0.2878867904511253 1104 58-59 0.34019548018250845 1104 60-61 0.3449434677755576 1104 62-63 0.3103352493596603 1104 64-65 0.31192954755503877 1104 66-67 0.22914935190357966 1104 68-69 0.26824899153066184 1104 70-71 0.19185420611670168 1104 72-73 0.31253575358332597 1104 74-75 0.3368537778841656 1104 76-77 0.2955529847251057 1104 78-79 0.3334586753239108 1104 80-81 0.42559286703234633 1104 82-83 0.3698299097729354 1104 84-85 0.27987686781037624 1104 86-87 0.4198246740912097 1104 88-89 0.3824018072654738 1104 90-91 0.579092288170763 1104 92-93 0.5658742957964371 1104 94-95 0.5586683354230075 1104 96-97 0.5341848466472037 1104 98-99 0.5763295650047162 1104 100-101 0.6560241040248975 1105 1 0.17660236223909465 1105 2 0.1451436047731285 1105 3 0.10550032027739675 1105 4 0.11313520605819605 1105 5 0.11655077593376717 1105 6 0.18559313013908252 1105 7 0.17672660740581136 1105 8 0.20029164779857211 1105 9 0.16322447441578447 1105 10-11 0.1420890477823562 1105 12-13 0.17844977669993511 1105 14-15 0.19959280378886746 1105 16-17 0.13006943927130266 1105 18-19 0.198631276141775 1105 20-21 0.1653111761992463 1105 22-23 0.23676884783226626 1105 24-25 0.14272618275324334 1105 26-27 0.19929411176414646 1105 28-29 0.15774383565765504 1105 30-31 0.1844088507432744 1105 32-33 0.2269552593911115 1105 34-35 0.23624022797984168 1105 36-37 0.2827238591832426 1105 38-39 0.32881157288005625 1105 40-41 0.3955583781635923 1105 42-43 0.2791584717833473 1105 44-45 0.21410710908179453 1105 46-47 0.25809279098627513 1105 48-49 0.2658786235242232 1105 50-51 0.28109812086388786 1105 52-53 0.2312269508348166 1105 54-55 0.24650304748015373 1105 56-57 0.1776842429600336 1105 58-59 0.26408564592943407 1105 60-61 0.22579913353093417 1105 62-63 0.21298987099847722 1105 64-65 0.2966946514746738 1105 66-67 0.2537482809483791 1105 68-69 0.21052963928288193 1105 70-71 0.22432601632861093 1105 72-73 0.22839499319203327 1105 74-75 0.3329486730412796 1105 76-77 0.27564943169294054 1105 78-79 0.24760673718487425 1105 80-81 0.2649811103458042 1105 82-83 0.29006246092520627 1105 84-85 0.41608253920008664 1105 86-87 0.4537493090265663 1105 88-89 0.42571998149655244 1105 90-91 0.42835057192590753 1105 92-93 0.35054130042736276 1105 94-95 0.3389527572573101 1105 96-97 0.4567031574562179 1105 98-99 0.5621679911976329 1105 100-101 0.637116347569954 1106 1 -0.09309427450936525 1106 2 -0.0342144021271551 1106 3 -0.11637195153178936 1106 4 0.03311467560674686 1106 5 0.005940492505004613 1106 6 0.03798944157958317 1106 7 0.07793286705383906 1106 8 0.02870525209475261 1106 9 -0.09310437706869834 1106 10-11 -0.0772608091731044 1106 12-13 -0.08265804956046452 1106 14-15 0.11764749549242737 1106 16-17 0.07141871205246275 1106 18-19 -0.025360093231519443 1106 20-21 0.008521215525540526 1106 22-23 -0.027878503656154408 1106 24-25 0.11295053481776307 1106 26-27 0.20204446550662425 1106 28-29 0.08910504564510546 1106 30-31 0.054008543519771024 1106 32-33 0.057698382761380174 1106 34-35 -0.030366518533554654 1106 36-37 -0.029060211391914947 1106 38-39 -0.03593830308687984 1106 40-41 -0.11719999440435913 1106 42-43 -0.08013948228536094 1106 44-45 -0.0631792044945314 1106 46-47 0.03970410571470495 1106 48-49 -0.014888725190807861 1106 50-51 -0.059158037415514286 1106 52-53 -0.19620702150854896 1106 54-55 -0.09536483909649718 1106 56-57 -0.10178603467910108 1106 58-59 -0.022847168696834785 1106 60-61 0.07918657366973036 1106 62-63 0.002873251105782515 1106 64-65 0.032430393994474116 1106 66-67 -0.014472832952471038 1106 68-69 0.09632085614682495 1106 70-71 -0.03687362688643958 1106 72-73 -0.03755181791593998 1106 74-75 -0.06944908989883913 1106 76-77 -0.19445517165372195 1106 78-79 -0.04679928353529306 1106 80-81 0.13029912981818015 1106 82-83 0.12664206589916915 1106 84-85 0.22068361591214014 1106 86-87 0.3689934824123213 1106 88-89 0.25346779177409573 1106 90-91 0.30606299918619584 1106 92-93 0.1364869909241868 1106 94-95 0.30702769980239353 1106 96-97 0.28256382615457554 1106 98-99 0.2744626706063862 1106 100-101 0.22177919636393284 1107 1 0.08777038348839739 1107 2 0.08891719394085129 1107 3 0.05510728486455818 1107 4 0.142577494408755 1107 5 0.02083358824917525 1107 6 -0.010794762300839977 1107 7 0.0633933481890736 1107 8 0.06145932554803579 1107 9 -0.03292762589749998 1107 10-11 0.0010418071905817783 1107 12-13 -0.04747384087369255 1107 14-15 -0.0033151862153104616 1107 16-17 0.12279946627200644 1107 18-19 0.00953178261178067 1107 20-21 -0.03281263732856843 1107 22-23 -0.1274161059641159 1107 24-25 -0.0832045340852332 1107 26-27 0.026850567491287336 1107 28-29 0.0429679776694698 1107 30-31 0.018855527123299964 1107 32-33 0.02291256150829213 1107 34-35 -0.014247730838405914 1107 36-37 0.05269638386184994 1107 38-39 0.05968182948312517 1107 40-41 -0.014309363457535085 1107 42-43 -0.02336321685285725 1107 44-45 -0.030749403151467902 1107 46-47 0.029822912638479693 1107 48-49 0.028477602359529897 1107 50-51 0.030964075481392683 1107 52-53 0.0520125621590779 1107 54-55 0.046431525504118554 1107 56-57 0.012061680391056484 1107 58-59 0.04074330094082512 1107 60-61 0.18226200096495404 1107 62-63 0.06132155502309189 1107 64-65 0.18496834001601314 1107 66-67 0.09585557491728736 1107 68-69 0.21398761275320766 1107 70-71 0.14737102318999717 1107 72-73 0.06688337556890644 1107 74-75 0.05935284045533962 1107 76-77 0.05255956713114429 1107 78-79 0.055060210694676925 1107 80-81 0.17538230239160768 1107 82-83 0.28318470521383077 1107 84-85 0.2528164860890243 1107 86-87 0.3301388724685168 1107 88-89 0.27353280378407163 1107 90-91 0.22324703471900875 1107 92-93 0.23753118848390287 1107 94-95 0.17464156364974315 1107 96-97 0.2323602827055744 1107 98-99 0.3739936465104563 1107 100-101 0.6258765099161856 1108 1 0.0038939536850044476 1108 2 0.005692781254946766 1108 3 -0.03213322804813146 1108 4 0.009188744681239314 1108 5 -0.06667663596847717 1108 6 -0.0581660733029139 1108 7 0.027857646605433217 1108 8 -0.0010184419891672292 1108 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-1.2963999289542123 2316 100-101 -1.6698108595420926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 155.0 3 109.0 4 294.0 5 817.0 6 1523.0 7 2681.0 8 4815.0 9 7416.0 10 10224.0 11 12825.0 12 16107.0 13 19170.0 14 22327.0 15 25170.0 16 29452.0 17 34086.0 18 39001.0 19 45041.0 20 48599.0 21 51413.0 22 57713.0 23 68733.0 24 84919.0 25 106684.0 26 136391.0 27 172557.0 28 217330.0 29 267780.0 30 327837.0 31 398535.0 32 484768.0 33 601280.0 34 770241.0 35 1067221.0 36 1387319.0 37 1953144.0 38 1839334.0 39 422007.0 40 953.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.905082602469626 31.02259787451193 28.759181718716814 22.31313780430163 2 22.54071848741022 29.22750070766771 28.21413172595194 20.01764907897013 3 21.17019503871611 28.439439711601306 28.5962303735731 21.79413487610948 4 21.639730259613383 28.70001609589577 28.398041069870345 21.2622125746205 5 22.15938129981696 28.612377859871813 28.407765812863666 20.82047502744756 6 21.974589096881257 28.55164707749651 27.95944508984093 21.514318735781302 7 22.155583318919174 28.781709637628495 28.08961574132419 20.973091302128143 8 21.4227691612974 28.692604874966364 28.2878899130594 21.596736050676835 9 21.17638960675332 28.512743140256188 28.74710563115474 21.563761621835756 10-11 21.289153161628498 28.95534052023747 28.178018505560164 21.57748781257386 12-13 21.287992608027036 29.000197467019746 28.054069636930805 21.657740288022413 14-15 21.445203435050743 28.978798794091208 27.911275269550647 21.664722501307406 16-17 21.59391380536243 28.800840948737793 27.882232713123862 21.72301253277592 18-19 21.596271375224724 28.80626331912844 27.86722837966466 21.730236925982183 20-21 21.674272644462697 28.742958189081026 27.89556335581804 21.687205810638236 22-23 21.646582973083376 28.742900545769196 27.88254340079966 21.727973080347766 24-25 21.655190760109168 28.72235776344776 27.8060922482 21.81635922824307 26-27 21.83042511154441 28.602868273529623 27.89360775534918 21.673098859576783 28-29 21.837836045321957 28.66114039475185 27.81875800423955 21.682265555686637 30-31 21.790049808058317 28.676864062367702 27.776354711096403 21.756731418477575 32-33 21.825602945994678 28.724291066472336 27.688470470543663 21.761635516989326 34-35 21.828593400153093 28.725055994134095 27.682465057108146 21.76388554860466 36-37 21.86008397960495 28.74302576206648 27.654811781734995 21.742078476593573 38-39 21.86453737353565 28.71550616663897 27.649845861969524 21.770110597855854 40-41 21.872398989810783 28.779545647721545 27.631311208373067 21.71674415409461 42-43 21.875710960361705 28.777975540704787 27.611615158963808 21.7346983399697 44-45 21.881101452204714 28.795242698167158 27.61507342059959 21.70858242902854 46-47 21.892298433097597 28.81565387768546 27.550779641325775 21.741268047891168 48-49 21.90696110999045 28.783601818436967 27.55914188785399 21.750295183718592 50-51 21.94313636777313 28.796806066606695 27.529576226008075 21.7304813396121 52-53 21.894628450712347 28.859003916683367 27.47894021742295 21.767427415181338 54-55 21.937170443698857 28.86832391019777 27.401940131659043 21.79256551444433 56-57 21.92351767716213 28.866098837263998 27.426555082907733 21.78382840266614 58-59 21.89882950920162 28.977414376246426 27.371070876571757 21.752685237980206 60-61 21.91944515765524 28.929896142174112 27.380670338249452 21.769988361921197 62-63 21.90621524071848 28.95980739000576 27.37818279925798 21.755794570017777 64-65 21.919003412981365 29.024182973021922 27.326252338935227 21.730561275061483 66-67 21.93012242238183 29.043784806394278 27.308733046200253 21.717359725023634 68-69 21.883853354188656 29.097555460702175 27.322710530844386 21.695880654264784 70-71 21.920404186784467 29.137765194367628 27.26537593256077 21.676454686287137 72-73 21.892813960824252 29.1389237505133 27.24184644343356 21.72641584522889 74-75 21.944393887204303 29.140307301498353 27.255068179620796 21.660230631676544 76-77 21.90016161556323 29.210534380169246 27.246548076554976 21.64275592771255 78-79 21.887577751467475 29.208745999779616 27.214361886782296 21.689314361970613 80-81 21.87136152841049 29.257624187264415 27.204375871603148 21.66663841272195 82-83 21.89877467760189 29.314615846757857 27.152641359171753 21.6339681164685 84-85 21.881254828109633 29.297641062535973 27.177149981932207 21.643954127422184 86-87 21.848489877021045 29.35438248382255 27.136003307097344 21.661124332059064 88-89 21.8483247991941 29.425266532241068 27.120832373732657 21.605576294832176 90-91 21.84599949720159 29.408113683484583 27.09938130087306 21.64650551844077 92-93 21.835054760386605 29.46051561449131 27.0670432781725 21.637386346949587 94-95 21.806789585227865 29.507449881207453 27.058908565367755 21.626851968196924 96-97 21.796194763112883 29.535288850802626 27.06469088999603 21.60382549608846 98-99 21.77802548088105 29.582647903948324 27.019647314621096 21.619679300549528 100-101 21.866723646455625 29.557020485479164 26.940643139807435 21.635612728257776 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 155512.0 1 88152.5 2 15615.0 3 9727.5 4 8645.5 5 7429.0 6 6549.5 7 6547.0 8 6503.5 9 5935.5 10 5470.5 11 6156.5 12 10392.0 13 10286.0 14 6623.0 15 7245.0 16 8393.5 17 10684.0 18 15545.5 19 18173.5 20 20618.5 21 24807.5 22 30632.0 23 39619.5 24 50121.5 25 61973.0 26 76766.5 27 93845.0 28 107805.5 29 123288.0 30 142933.0 31 162583.0 32 181158.5 33 199670.5 34 220033.0 35 240403.0 36 262803.0 37 287325.0 38 305796.5 39 315613.0 40 329468.5 41 346020.0 42 356748.0 43 365913.0 44 372975.5 45 380826.5 46 389020.0 47 394822.5 48 402681.0 49 408416.0 50 405686.0 51 396371.0 52 385027.5 53 366319.0 54 336349.0 55 311052.5 56 283938.5 57 250085.0 58 224202.5 59 195776.5 60 160654.5 61 125887.5 62 91650.0 63 62788.5 64 42418.0 65 27580.5 66 17363.5 67 11766.5 68 7250.0 69 3682.0 70 1695.0 71 774.0 72 375.5 73 209.5 74 162.5 75 112.0 76 70.5 77 51.0 78 38.5 79 30.5 80 19.0 81 14.5 82 14.0 83 12.0 84 7.5 85 5.5 86 6.0 87 4.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14802573516638598 2 0.0 3 0.0 4 0.0029340615767311593 5 4.0983717262276507E-4 6 9.966494879689969E-4 7 0.0 8 1.8628962391943867E-4 9 3.5395028544693346E-4 10-11 7.917309016576144E-5 12-13 9.314481195971935E-6 14-15 1.39717217939579E-5 16-17 1.39717217939579E-5 18-19 1.862896239194387E-5 20-21 6.98586089697895E-5 22-23 2.0957582690936852E-4 24-25 0.0 26-27 4.657240597985967E-6 28-29 0.006003183130803912 30-31 0.0014716880289635656 32-33 3.586075260449195E-4 34-35 2.3286202989929836E-4 36-37 2.8874891707512994E-4 38-39 0.0012947128862400988 40-41 0.0033392415087559386 42-43 4.890102627885265E-4 44-45 4.983247439844984E-4 46-47 0.006650539573923961 48-49 0.006589995446150144 50-51 0.006929974009803119 52-53 0.009272566030590061 54-55 0.004787643334729573 56-57 0.0 58-59 4.1915165381873704E-5 60-61 8.615895106274039E-4 62-63 7.405012550797688E-4 64-65 4.6106681920061077E-4 66-67 6.194129995321336E-4 68-69 9.640488037830952E-4 70-71 0.0013273135704260006 72-73 0.0025381961259023523 74-75 6.054412777381757E-4 76-77 0.0 78-79 0.0 80-81 6.520136837180353E-5 82-83 1.9560410511541062E-4 84-85 1.3505997734159303E-4 86-87 0.0025381961259023523 88-89 0.0015694900815212708 90-91 5.402399093663721E-4 92-93 5.821550747482459E-4 94-95 2.6080547348721414E-4 96-97 0.0010106212097629548 98-99 0.0 100-101 4.657240597985967E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0735971E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.28070139900355 #Duplication Level Percentage of deduplicated Percentage of total 1 88.46914734154643 66.60019464043405 2 6.994820624659657 10.531500055691904 3 1.8795229154587016 4.244754101136934 4 0.86649614131267 2.6092174911019157 5 0.45855958057185225 1.7260343429340959 6 0.27348791587962357 1.2353017278941845 7 0.17908938707086194 0.9437382270268487 8 0.11988070673300537 0.7219762949655113 9 0.08299264859175978 0.5622970317253829 >10 0.6694572394478375 9.173659667486762 >50 0.005558050422829828 0.24758862866094333 >100 7.194877672253827E-4 0.09748336233989924 >500 6.699014901913032E-5 0.03373810563846794 >1k 6.699012950529861E-5 0.11021616232060147 >5k 6.699012950529834E-5 0.2916908180564436 >10k+ 6.699012950529834E-5 0.8706093425859982 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23779 0.22148904835901664 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10748 0.10011204389430634 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.314481195971935E-6 0.0 0.0 0.0 0.0 2 9.314481195971935E-6 0.0 0.0 9.314481195971935E-6 0.0 3 9.314481195971935E-6 0.0 0.0 1.862896239194387E-5 0.0 4 9.314481195971935E-6 0.0 0.0 1.862896239194387E-5 3.725792478388774E-5 5 9.314481195971935E-6 0.0 0.0 1.862896239194387E-5 7.451584956777548E-5 6 9.314481195971935E-6 0.0 0.0 1.862896239194387E-5 9.314481195971934E-5 7 9.314481195971935E-6 9.314481195971935E-6 0.0 3.725792478388774E-5 1.1177377435166321E-4 8 9.314481195971935E-6 9.314481195971935E-6 0.0 3.725792478388774E-5 1.3040273674360707E-4 9 9.314481195971935E-6 1.862896239194387E-5 0.0 3.725792478388774E-5 1.5834618033152287E-4 10-11 9.314481195971935E-6 1.862896239194387E-5 0.0 3.725792478388774E-5 1.862896239194387E-4 12-13 9.314481195971935E-6 1.862896239194387E-5 0.0 3.725792478388774E-5 2.4683375169325626E-4 14-15 1.862896239194387E-5 1.862896239194387E-5 0.0 4.657240597985967E-5 2.9340615767311595E-4 16-17 1.862896239194387E-5 4.657240597985967E-5 0.0 4.657240597985967E-5 3.4463580425096155E-4 18-19 1.862896239194387E-5 8.383033076374741E-5 0.0 4.657240597985967E-5 3.6326476664290543E-4 20-21 1.862896239194387E-5 1.0711653375367725E-4 0.0 4.657240597985967E-5 4.0983717262276507E-4 22-23 1.862896239194387E-5 1.257454961456211E-4 0.0 4.657240597985967E-5 4.517523380046388E-4 24-25 1.862896239194387E-5 1.3040273674360707E-4 0.0 6.054412777381757E-5 4.796957815925546E-4 26-27 2.3286202989929838E-5 1.3040273674360707E-4 0.0 6.520136837180353E-5 4.936675033865125E-4 28-29 3.725792478388774E-5 1.3505997734159305E-4 0.0 7.451584956777548E-5 5.402399093663722E-4 30-31 4.657240597985967E-5 1.6300342092950886E-4 0.0 7.451584956777548E-5 5.495543905623441E-4 32-33 7.451584956777548E-5 1.8628962391943867E-4 0.0 7.451584956777548E-5 5.914695559442178E-4 34-35 7.451584956777548E-5 1.9560410511541062E-4 0.0 7.451584956777548E-5 6.194129995321337E-4 36-37 8.848757136173337E-5 2.0957582690936852E-4 0.0 7.451584956777548E-5 6.566709243160213E-4 38-39 1.0245929315569127E-4 2.2354754870332642E-4 0.0 7.917309016576145E-5 6.706426461099793E-4 40-41 1.3040273674360707E-4 2.3286202989929834E-4 0.0 8.383033076374741E-5 6.985860896978951E-4 42-43 1.5368893973353691E-4 2.3286202989929834E-4 0.0 8.383033076374741E-5 7.358440144817828E-4 44-45 1.5834618033152287E-4 2.4217651109527028E-4 0.0 8.383033076374741E-5 7.777591798636566E-4 46-47 1.7697514272346673E-4 2.701199546831861E-4 0.0 8.848757136173337E-5 8.150171046475442E-4 48-49 2.1423306750735448E-4 2.8874891707512994E-4 0.0 9.314481195971934E-5 8.289888264415022E-4 50-51 2.4683375169325626E-4 2.8874891707512994E-4 0.0 9.780205255770531E-5 8.895329542153197E-4 52-53 2.84091676477144E-4 2.8874891707512994E-4 0.0 1.1177377435166321E-4 9.314481195971933E-4 54-55 2.9340615767311595E-4 2.9806339827110186E-4 0.0 1.1643101494964917E-4 9.593915631851092E-4 56-57 3.260068418590177E-4 3.1669236066304574E-4 0.0 1.257454961456211E-4 0.0010013067285669828 58-59 3.6326476664290543E-4 3.213496012610317E-4 0.0 1.4437445853756497E-4 0.0010432218939488568 60-61 3.865509696328353E-4 3.3066408245700367E-4 0.0 1.583461803315229E-4 0.0010711653375367724 62-63 4.23808894416723E-4 3.4463580425096155E-4 0.0 1.6766066152749482E-4 0.0011037660217226742 64-65 4.890102627885265E-4 3.4463580425096155E-4 0.0 1.6766066152749482E-4 0.0011549956683005198 66-67 5.448971499643581E-4 3.586075260449195E-4 0.0 1.6766066152749482E-4 0.0012062253148783656 68-69 6.147557589341475E-4 3.7257924783887735E-4 0.0 1.7231790212548077E-4 0.0012853984050441268 70-71 7.311867738837969E-4 3.7257924783887735E-4 0.0 1.8163238332145272E-4 0.0013179990892300286 72-73 8.103598640495583E-4 3.772364884368633E-4 0.0 1.9560410511541062E-4 0.0013505997734159303 74-75 9.035046760092776E-4 3.818937290348493E-4 0.0 2.0026134571339658E-4 0.0013971721793957902 76-77 0.0010292501721548986 3.818937290348493E-4 0.0 2.0491858631138256E-4 0.001429772863581692 78-79 0.001257454961456211 3.9120821023082124E-4 0.0 2.0957582690936852E-4 0.0014623735477675935 80-81 0.0014530590665716215 4.098371726227651E-4 0.0 2.1423306750735448E-4 0.0014996314725514814 82-83 0.0018070093520185552 4.098371726227651E-4 0.0 2.1423306750735448E-4 0.001541546637933355 84-85 0.002254104449425208 4.098371726227651E-4 0.0 2.2354754870332642E-4 0.0015788045627172429 86-87 0.002850231245967412 4.1915165381873704E-4 0.0 2.2354754870332642E-4 0.0016207197280991167 88-89 0.003809622809152521 4.4243785680866683E-4 0.0 2.2354754870332642E-4 0.0016533204122850184 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 485 0.0 31.340204 3 CGCGTAG 285 0.0 26.666605 54-55 TCGCGTA 395 0.0 20.443848 52-53 CAACGCA 7510 0.0 20.270266 12-13 CGTACGC 355 0.0 20.069534 20-21 CTCGCGT 370 0.0 19.899437 52-53 ATCAACG 7745 0.0 19.655222 10-11 ACTCGCG 375 0.0 19.634613 50-51 ACGTACG 400 0.0 18.999159 20-21 AGTGGTA 9855 0.0 17.302916 5 TCAACGC 7600 0.0 16.655396 10-11 GTATCAA 10480 0.0 16.315958 9 CGTAGCG 500 0.0 16.149473 56-57 GGTATCA 10600 0.0 15.952013 8 AACGCAG 8085 0.0 15.685653 12-13 TGGTATC 11235 0.0 15.38862 7 ATCGGAA 2000 0.0 14.9625 2 CGCTAGT 510 0.0 14.901406 90-91 AAGCAGT 11760 0.0 14.683259 1 ACGCAGA 10665 0.0 14.385171 14-15 >>END_MODULE